US20100316751A1 - Swinglea glutinosa LEAVES DERIVED MATERIAL, ALONE OR IN COMBINATION WITH COPPER SULFATE, FOR CONTROL OF SOUR ROT GRAPES AND BUNCH ROT GRAPES - Google Patents
Swinglea glutinosa LEAVES DERIVED MATERIAL, ALONE OR IN COMBINATION WITH COPPER SULFATE, FOR CONTROL OF SOUR ROT GRAPES AND BUNCH ROT GRAPES Download PDFInfo
- Publication number
- US20100316751A1 US20100316751A1 US12/860,896 US86089610A US2010316751A1 US 20100316751 A1 US20100316751 A1 US 20100316751A1 US 86089610 A US86089610 A US 86089610A US 2010316751 A1 US2010316751 A1 US 2010316751A1
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- Prior art keywords
- leaves
- extract
- grapes
- solvent
- swinglea glutinosa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 241000059151 Swinglea glutinosa Species 0.000 title claims abstract description 44
- 241000219094 Vitaceae Species 0.000 title claims abstract description 32
- 235000021021 grapes Nutrition 0.000 title claims abstract description 32
- 239000000463 material Substances 0.000 title claims abstract description 17
- 229910000365 copper sulfate Inorganic materials 0.000 title claims abstract description 15
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 title claims abstract description 15
- 241000123650 Botrytis cinerea Species 0.000 claims abstract description 20
- 238000002360 preparation method Methods 0.000 claims abstract description 18
- 239000002904 solvent Substances 0.000 claims description 30
- 239000012634 fragment Substances 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 9
- 239000000575 pesticide Substances 0.000 abstract description 11
- 241001558165 Alternaria sp. Species 0.000 abstract description 5
- 241000228257 Aspergillus sp. Species 0.000 abstract description 5
- 241001207508 Cladosporium sp. Species 0.000 abstract description 5
- 241001558145 Mucor sp. Species 0.000 abstract description 5
- 241000228168 Penicillium sp. Species 0.000 abstract description 5
- 241000952054 Rhizopus sp. Species 0.000 abstract description 5
- 239000005445 natural material Substances 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 235000014787 Vitis vinifera Nutrition 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 6
- 241000219095 Vitis Species 0.000 description 6
- 235000009754 Vitis X bourquina Nutrition 0.000 description 6
- 235000012333 Vitis X labruscana Nutrition 0.000 description 6
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000011593 sulfur Substances 0.000 description 6
- 229910052717 sulfur Inorganic materials 0.000 description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 229910052802 copper Inorganic materials 0.000 description 4
- 239000010949 copper Substances 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- VDLGAVXLJYLFDH-UHFFFAOYSA-N fenhexamid Chemical compound C=1C=C(O)C(Cl)=C(Cl)C=1NC(=O)C1(C)CCCCC1 VDLGAVXLJYLFDH-UHFFFAOYSA-N 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 239000005758 Cyprodinil Substances 0.000 description 2
- 239000005776 Fenhexamid Substances 0.000 description 2
- 239000005781 Fludioxonil Substances 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- HAORKNGNJCEJBX-UHFFFAOYSA-N cyprodinil Chemical compound N=1C(C)=CC(C2CC2)=NC=1NC1=CC=CC=C1 HAORKNGNJCEJBX-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- MUJOIMFVNIBMKC-UHFFFAOYSA-N fludioxonil Chemical compound C=12OC(F)(F)OC2=CC=CC=1C1=CNC=C1C#N MUJOIMFVNIBMKC-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 1
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 229940093499 ethyl acetate Drugs 0.000 description 1
- 235000019439 ethyl acetate Nutrition 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- -1 nitrile-acetate Chemical compound 0.000 description 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/36—Rutaceae [Rue family], e.g. lime, orange, lemon, corktree or pricklyash
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/10—Anthelmintics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the present invention is related to how to treat Botrytis cinerea bunch rot grapes and sour rot grapes with an extract material derived from Swinglea glutinosa leaves and a preparation made of the material derived from Swinglea glutinosa leaves and copper sulfate.
- the extract is described by preceding pending unpublished U.S. patent application Ser. No. 12/466,801.
- Botrytis cinerea affects grape cultivation causing bunch rot to grapes resulting in great losses for wineries.
- Another winery loss known malady to grape cultivation is commonly called sour rot which is caused by a complex of microorganisms that include Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp.
- Botrytis cinerea bunch rot and sour rot are hard to control and usually require rotation or mixes of multiple pesticides, e.g., a mix of fenhexamid and sulfur, or a mix of cyprodinil, fludioxonil, and sulfur, or a mix of copper oxichloride, copper sulfate, copper, and sulfur.
- multiple pesticides e.g., a mix of fenhexamid and sulfur, or a mix of cyprodinil, fludioxonil, and sulfur, or a mix of copper oxichloride, copper sulfate, copper, and sulfur.
- the invention of the present application provides a natural material derived from Swinglea glutinosa leaves that alone or in combination with copper sulfate is as good or better than pesticide combinations for controlling Botrytis cinerea bunch rot of grapes, and sour rot of grapes caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp.
- the material derived from Swinglea glutinosa leaves, by itself, has better results than any of the pesticide combinations for controlling both, sour rot and bunch rot.
- a preparation of the material derived from Swinglea glutinosa leaves in combination with copper sulfate is better than pesticide combinations for sour rot, and a very good alternative, instead of pesticide combinations, for Botrytis cinerea bunch rot.
- the invention of the present application provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat sour rot grapes, wherein the sour rot grapes are caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp.
- the extract used to treat sour rot grapes is obtained by a method comprising.
- the invention of the present application also provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat Botrytis cinerea bunch rot grapes.
- the extract used to treat Botrytis cinerea bunch rot grapes is obtained by a method comprising:
- the invention of the present application provides a preparation that comprises a material derived from Swinglea glutinosa leaves and copper sulfate.
- the preparation is used to treat sour rot grapes.
- the preparation is used to treat Botrytis cinerea bunch rot grapes.
- the material derived from Swinglea glutinosa leaves is an extract obtained by a method comprising:
- the invention of the present application provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat sour rot grapes.
- sour rot grapes are caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., Penicilium sp., and Acetobacter , sp.
- the extract used to treat sour rot grapes is obtained by a method comprising.
- the invention of the present application also provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat Botrytis cinerea bunch rot grapes.
- the extract used to treat Botrytis cinerea bunch rot grapes is obtained by a method comprising:
- the invention of the present application provides a preparation that comprises a material derived from Swinglea glutinosa leaves and copper sulfate.
- the preparation is used to treat sour rot grapes.
- the preparation is used to treat Botrytis cinerea bunch rot grapes.
- the material derived from Swinglea glutinosa leaves is an extract obtained by a method comprising:
- the leaves in the method, must not be broken into leaf fragments that are less than 0.5 mm, since smaller fragments would tend to become a single mass which will become a limitation for the optimal extraction with a solvent.
- the solvent that can be used comprises ethanol, methanol, hexane, propanol, isopropanol, CO 2 , acetone, water, ethyl-acetate, nitrile-acetate, toluene, tetrahydrofurane, Chloroform, dichloromethane, and others.
- 25 grape bunches from each of the four grape vines were chosen randomly. There were two factors evaluated: incidence of diseases and degree of disease. Incidence was measured according to the percentage of grape bunches affected in relation of total of evaluated grape bunches, and degree of disease was measured according to percentage of grapes in each bunch affected for the 25 grape bunches. There was no phytotoxicity observed for treatments I. and II.
- Table 1 shows that the best results for both, sour rot and Botrytis cinerea bunch rot was achieved when vines were treated with I. (an extract obtained from Swinglea glutinosa leaves diluted in water at a concentration of 200 cc of extract/100 liters of water). It also shows that II. (Swinglea glutinosa extract 100 cc/100Liters of water+Copper Sulfate) is better than III. (pesticide mixes) for sour rot, and that II. is a good alternative instead of III. for Botrytis cinerea bunch rot when compared to IV. (No treatment).
- Swinglea glutinosa derived extract alone or in combination with copper sulfate decreased incidence and degree of disease for sour rot and Botrytis cinerea bunch rot.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Peptides Or Proteins (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
The invention of the present application provides a natural material derived from Swinglea glutinosa leaves that alone or in combination with copper sulfate is as good or better than pesticide combinations for controlling Botrytis cinerea bunch rot of grapes, and sour rot of grapes caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp. The material derived from Swinglea glutinosa leaves, by itself, has better results than any of the pesticide combinations for controlling both, sour rot and bunch rot. A preparation of the material derived from Swinglea glutinosa leaves in combination with copper sulfate is better than pesticide combinations for sour rot of grapes, and a very good alternative, instead of pesticide combinations, for Botrytis cinerea bunch rot of grapes.
Description
- This application is a continuation in part of U.S. patent application Ser. No. 12/466,801, which has one inventor in common. U.S. patent application Ser. No. 12/466,801 is incorporated herein in its entirety by reference.
- 1. Area of the Invention
- The present invention is related to how to treat Botrytis cinerea bunch rot grapes and sour rot grapes with an extract material derived from Swinglea glutinosa leaves and a preparation made of the material derived from Swinglea glutinosa leaves and copper sulfate. The extract is described by preceding pending unpublished U.S. patent application Ser. No. 12/466,801.
- 2. Description of Prior Art
- Botrytis cinerea affects grape cultivation causing bunch rot to grapes resulting in great losses for wineries. Another winery loss known malady to grape cultivation is commonly called sour rot which is caused by a complex of microorganisms that include Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp.
- Both, Botrytis cinerea bunch rot and sour rot are hard to control and usually require rotation or mixes of multiple pesticides, e.g., a mix of fenhexamid and sulfur, or a mix of cyprodinil, fludioxonil, and sulfur, or a mix of copper oxichloride, copper sulfate, copper, and sulfur.
- Unfortunately, there is no description in the prior art of a natural derived compound that alone or in a mix can diminish the use of the mentioned pesticides for control of Botrytis cinerea bunch rot and sour rot. The Invention of the present Application overcomes these prior art limitations.
- The invention of the present application provides a natural material derived from Swinglea glutinosa leaves that alone or in combination with copper sulfate is as good or better than pesticide combinations for controlling Botrytis cinerea bunch rot of grapes, and sour rot of grapes caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp. The material derived from Swinglea glutinosa leaves, by itself, has better results than any of the pesticide combinations for controlling both, sour rot and bunch rot. A preparation of the material derived from Swinglea glutinosa leaves in combination with copper sulfate is better than pesticide combinations for sour rot, and a very good alternative, instead of pesticide combinations, for Botrytis cinerea bunch rot.
- More specifically, the invention of the present application provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat sour rot grapes, wherein the sour rot grapes are caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., and Penicilium sp.
- In one aspect of the present invention, the extract used to treat sour rot grapes is obtained by a method comprising.
-
- A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
- B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
- C. breaking up the leaves into small fragments;
- D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
- E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
- A. retiring the solvent to release an extract.
- The invention of the present application also provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat Botrytis cinerea bunch rot grapes.
- In an aspect of the present invention the extract used to treat Botrytis cinerea bunch rot grapes is obtained by a method comprising:
-
- A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
- B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
- C. breaking up the leaves into small fragments;
- D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
- E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
- F. retiring the solvent to release an extract.
- In addition, the invention of the present application provides a preparation that comprises a material derived from Swinglea glutinosa leaves and copper sulfate.
- In another aspect of the present invention, the preparation is used to treat sour rot grapes.
- In one additional aspect of the present invention, the preparation is used to treat Botrytis cinerea bunch rot grapes.
- In one more aspect of the preparation of the present invention, the material derived from Swinglea glutinosa leaves is an extract obtained by a method comprising:
-
- A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
- B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
- C. breaking up the leaves into small fragments;
- D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
- E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
- F. retiring the solvent to release an extract.
- Objectives and advantages of the present Application invention will be more evident in the detailed description of the invention and the claims.
- The invention of the present application provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat sour rot grapes.
- In all cases in the present application, sour rot grapes are caused by Alternaria sp., Cladosporium sp., Aspergillus sp., Mucor sp., Rhizopus sp., Penicilium sp., and Acetobacter, sp.
- In one aspect of the present invention, the extract used to treat sour rot grapes is obtained by a method comprising.
-
- A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
- B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
- C. breaking up the leaves into small fragments;
- D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
- E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
- B. retiring the solvent to release an extract.
- The invention of the present application also provides a material extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat Botrytis cinerea bunch rot grapes.
- In an aspect of the present invention the extract used to treat Botrytis cinerea bunch rot grapes is obtained by a method comprising:
-
- A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
- B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
- C. breaking up the leaves into small fragments;
- D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
- E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
- F. retiring the solvent to release an extract.
- In addition, the invention of the present application provides a preparation that comprises a material derived from Swinglea glutinosa leaves and copper sulfate.
- In another aspect of the present invention, the preparation is used to treat sour rot grapes.
- In one additional aspect of the present invention, the preparation is used to treat Botrytis cinerea bunch rot grapes.
- In one more aspect of the preparation of the present invention, the material derived from Swinglea glutinosa leaves is an extract obtained by a method comprising:
-
- A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
- B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
- C. breaking up the leaves into small fragments;
- D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
- E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
- F. retiring the solvent to release an extract.
- In one more aspect of the Swinglea glutinosa extract of the present invention, in the method, the leaves must not be broken into leaf fragments that are less than 0.5 mm, since smaller fragments would tend to become a single mass which will become a limitation for the optimal extraction with a solvent.
- In all cases where a solvent is mentioned in this application, the solvent that can be used comprises ethanol, methanol, hexane, propanol, isopropanol, CO2, acetone, water, ethyl-acetate, nitrile-acetate, toluene, tetrahydrofurane, Chloroform, dichloromethane, and others.
- Objectives and advantages of the present Application invention will be more evident in the detailed description of the invention and the claims.
- Four vines of Red Globe grapes were treated for each experiment 6 times every 7 days (each experiment was repeated four times) as follows:
- I. With an extract obtained from Swinglea glutinosa leaves, wherein the extract was diluted in water at a concentration of 200 cc of extract/100 liters of water.
- II. With a preparation of an extract obtained from Swinglea Glutinosa leaves in combination with copper sulfate, where the extract was used in a concentration of 100 cc of extract/100 liters of water in a mix that also deliver a 1 kg/ha of Copper sulfate.
- III. One of the following chosen randomly (according to traditional treatment): a mix of fenhexamid and sulfur (Teldor® 3.4%, 20 kg/ha); or a mix of cyprodinil, fludioxonil, and sulfur (Switch Dry® 18 kg/ha); or a mix of copper oxichloride, copper sulfate, copper, and sulfur (Custdust® 20 kg/ha).
- IV. No treatment.
- In all cases liquid treatments were made with a 200 liter piton pump equivalent to a water volume of 2000 liters/ha. Powder treatment were made with a dry powder pump. The last application out of six was made 10 days before harvesting.
- For evaluation, 25 grape bunches from each of the four grape vines were chosen randomly. There were two factors evaluated: incidence of diseases and degree of disease. Incidence was measured according to the percentage of grape bunches affected in relation of total of evaluated grape bunches, and degree of disease was measured according to percentage of grapes in each bunch affected for the 25 grape bunches. There was no phytotoxicity observed for treatments I. and II.
- Results are described in the Following table:
-
TABLE 1 INCIDENCE AND DISEASE DEGREE FOR SOUR ROT AND Botrytis cinerea BUNCH ROT IN RED GLOBE GRAPES INCIDENCE (%) DISEASE DEGREE (%) TREATMENT* Sour Rot Bunch Rot Sour Rot Bunch Rot I. 31 2 60 10 II. 29 6 56 28 III. 41 3 69 16 IV. 68 14 100 47 *I, II, III y IV explained in paragraphs 0028-0031 - Table 1. shows that the best results for both, sour rot and Botrytis cinerea bunch rot was achieved when vines were treated with I. (an extract obtained from Swinglea glutinosa leaves diluted in water at a concentration of 200 cc of extract/100 liters of water). It also shows that II. (Swinglea glutinosa extract 100 cc/100Liters of water+Copper Sulfate) is better than III. (pesticide mixes) for sour rot, and that II. is a good alternative instead of III. for Botrytis cinerea bunch rot when compared to IV. (No treatment).
- In conclusion, the use of Swinglea glutinosa derived extract alone or in combination with copper sulfate decreased incidence and degree of disease for sour rot and Botrytis cinerea bunch rot.
Claims (8)
1. An extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat sour rot grapes.
2. The extract of claim 1 , wherein the extract is obtained by a method comprising.
A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
C. breaking up the leaves into small fragments;
D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
C. retiring the solvent to release an extract.
3. An extract isolated from Swinglea glutinosa leaves, wherein the extract is used to treat Botrytis cinerea bunch rot grapes.
4. The extract of claim 3 , wherein the extract is obtained by a method comprising:
A. Exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
C. breaking up the leaves into small fragments;
D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
F. retiring the solvent to release an extract.
5. A preparation that comprises a material derived from Swinglea glutinosa leaves and copper sulfate.
6. The preparation of claim 5 , wherein the preparation is used to treat sour rot grapes.
7. The preparation of claim 5 , wherein the preparation is used to treat Botrytis cinerea bunch rot grapes.
8. The preparation of claim 5 , wherein the material is an extract obtained by a method comprising:
A. exposing bright dark green Swinglea glutinosa leaves in a dry environment, wherein said leaves are protected from direct sunlight, wherein the leaves are exposed to air flow;
B. leaving the leaves in the environment described in A. until the leaves turn opaque green and the leaves are brittle;
C. breaking up the leaves into small fragments;
D. putting the Swinglea glutinosa leaf fragments in contact with a solvent at least once;
E. repeat step D. until the solvent is enriched with substances derived from the leaf fragments; and,
F. retiring the solvent to release an extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/860,896 US20100316751A1 (en) | 2009-05-15 | 2010-08-22 | Swinglea glutinosa LEAVES DERIVED MATERIAL, ALONE OR IN COMBINATION WITH COPPER SULFATE, FOR CONTROL OF SOUR ROT GRAPES AND BUNCH ROT GRAPES |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/466,801 US20100291241A1 (en) | 2009-05-15 | 2009-05-15 | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US12/860,896 US20100316751A1 (en) | 2009-05-15 | 2010-08-22 | Swinglea glutinosa LEAVES DERIVED MATERIAL, ALONE OR IN COMBINATION WITH COPPER SULFATE, FOR CONTROL OF SOUR ROT GRAPES AND BUNCH ROT GRAPES |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US12/466,801 Continuation-In-Part US20100291241A1 (en) | 2009-05-15 | 2009-05-15 | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US20100316751A1 true US20100316751A1 (en) | 2010-12-16 |
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Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US12/466,801 Abandoned US20100291241A1 (en) | 2009-05-15 | 2009-05-15 | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US12/860,896 Abandoned US20100316751A1 (en) | 2009-05-15 | 2010-08-22 | Swinglea glutinosa LEAVES DERIVED MATERIAL, ALONE OR IN COMBINATION WITH COPPER SULFATE, FOR CONTROL OF SOUR ROT GRAPES AND BUNCH ROT GRAPES |
| US15/056,303 Active 2031-02-16 US10470467B2 (en) | 2009-05-15 | 2016-02-29 | Method of production of extract derived from Swinglea glutinosa leaves |
| US16/653,044 Abandoned US20200037615A1 (en) | 2009-05-15 | 2019-10-15 | Method of production of extract derived from swinglea glutinosa leaves |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US12/466,801 Abandoned US20100291241A1 (en) | 2009-05-15 | 2009-05-15 | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US15/056,303 Active 2031-02-16 US10470467B2 (en) | 2009-05-15 | 2016-02-29 | Method of production of extract derived from Swinglea glutinosa leaves |
| US16/653,044 Abandoned US20200037615A1 (en) | 2009-05-15 | 2019-10-15 | Method of production of extract derived from swinglea glutinosa leaves |
Country Status (16)
| Country | Link |
|---|---|
| US (4) | US20100291241A1 (en) |
| EP (1) | EP2429488B1 (en) |
| JP (1) | JP2012526794A (en) |
| CN (1) | CN102791252A (en) |
| AP (1) | AP2011006030A0 (en) |
| AR (1) | AR076874A1 (en) |
| AU (1) | AU2010247098A1 (en) |
| BR (1) | BRPI1007665A2 (en) |
| CA (1) | CA2761508A1 (en) |
| CL (1) | CL2011002841A1 (en) |
| CO (1) | CO6470891A2 (en) |
| EA (1) | EA201190250A1 (en) |
| ES (1) | ES2772049T3 (en) |
| MX (1) | MX2011012121A (en) |
| PE (1) | PE20120904A1 (en) |
| WO (1) | WO2010131109A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100291241A1 (en) * | 2009-05-15 | 2010-11-18 | Jaime Toro Restrepo | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US20110020481A1 (en) * | 2009-05-15 | 2011-01-27 | Jamez Alberto Jimenez Martinez | Swinglea glutinosa LEAVES DERIVED MATERIAL IN COMBINATION WITH AVERMECTINS FOR CONTROL OF ACARI |
| WO2012063121A1 (en) * | 2010-11-14 | 2012-05-18 | Jimenez James A | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8298590B2 (en) * | 2010-05-10 | 2012-10-30 | Jaime Toro Restrepo | Method for extraction of material from a Sapindacea family fruit |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7297349B2 (en) * | 2002-08-26 | 2007-11-20 | Riken | Copper-containing formulation for plant disease control |
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|---|---|---|---|---|
| HU206605B (en) * | 1989-07-31 | 1992-12-28 | Chinoin Gyogyszer Es Vegyeszet | Synergetic artropodicide compositions containing pyrethroides as active components |
| US5498624A (en) * | 1995-05-03 | 1996-03-12 | Monsanto Company | Selected pyrazolyl derivatives |
| HU229710B1 (en) * | 1995-07-12 | 2014-05-28 | Du Pont | Fungicidal mixtures |
| US20020031538A1 (en) * | 2000-09-11 | 2002-03-14 | Scarmoutzos Louis Michael | Light activateable natural pesticide formulations |
| US20100291241A1 (en) | 2009-05-15 | 2010-11-18 | Jaime Toro Restrepo | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US8993011B2 (en) * | 2009-05-15 | 2015-03-31 | Ecoflora Agro Sas | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
| US8932654B2 (en) * | 2009-05-15 | 2015-01-13 | Ecoflora Agro S.A.S. | Swinglea glutinosa leaves derived material in combination with avermectins for control of acari |
-
2009
- 2009-05-15 US US12/466,801 patent/US20100291241A1/en not_active Abandoned
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2010
- 2010-05-14 BR BRPI1007665A patent/BRPI1007665A2/en not_active IP Right Cessation
- 2010-05-14 ES ES10774608T patent/ES2772049T3/en active Active
- 2010-05-14 JP JP2012510382A patent/JP2012526794A/en active Pending
- 2010-05-14 WO PCT/IB2010/001129 patent/WO2010131109A1/en active Application Filing
- 2010-05-14 PE PE2011001943A patent/PE20120904A1/en not_active Application Discontinuation
- 2010-05-14 MX MX2011012121A patent/MX2011012121A/en active IP Right Grant
- 2010-05-14 AP AP2011006030A patent/AP2011006030A0/en unknown
- 2010-05-14 AU AU2010247098A patent/AU2010247098A1/en not_active Abandoned
- 2010-05-14 EA EA201190250A patent/EA201190250A1/en unknown
- 2010-05-14 EP EP10774608.3A patent/EP2429488B1/en active Active
- 2010-05-14 AR ARP100101695A patent/AR076874A1/en unknown
- 2010-05-14 CN CN2010800209591A patent/CN102791252A/en active Pending
- 2010-05-14 CA CA2761508A patent/CA2761508A1/en not_active Abandoned
- 2010-08-22 US US12/860,896 patent/US20100316751A1/en not_active Abandoned
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2016
- 2016-02-29 US US15/056,303 patent/US10470467B2/en active Active
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2019
- 2019-10-15 US US16/653,044 patent/US20200037615A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7297349B2 (en) * | 2002-08-26 | 2007-11-20 | Riken | Copper-containing formulation for plant disease control |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100291241A1 (en) * | 2009-05-15 | 2010-11-18 | Jaime Toro Restrepo | METHOD OF PRODUCTION OF EXTRACT DERIVED FROM Swinglea Glutinosa LEAVES |
| US20110020481A1 (en) * | 2009-05-15 | 2011-01-27 | Jamez Alberto Jimenez Martinez | Swinglea glutinosa LEAVES DERIVED MATERIAL IN COMBINATION WITH AVERMECTINS FOR CONTROL OF ACARI |
| US8932654B2 (en) | 2009-05-15 | 2015-01-13 | Ecoflora Agro S.A.S. | Swinglea glutinosa leaves derived material in combination with avermectins for control of acari |
| US8993011B2 (en) | 2009-05-15 | 2015-03-31 | Ecoflora Agro Sas | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
| US10470467B2 (en) | 2009-05-15 | 2019-11-12 | Gowan Crop Protection Limited | Method of production of extract derived from Swinglea glutinosa leaves |
| WO2012063121A1 (en) * | 2010-11-14 | 2012-05-18 | Jimenez James A | Swinglea glutinosa leaves derived material in combination with pyrethroids for control of acari |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2772049T3 (en) | 2020-07-07 |
| US10470467B2 (en) | 2019-11-12 |
| EP2429488B1 (en) | 2019-11-13 |
| AR076874A1 (en) | 2011-07-13 |
| US20200037615A1 (en) | 2020-02-06 |
| PE20120904A1 (en) | 2012-08-02 |
| WO2010131109A9 (en) | 2010-12-23 |
| EA201190250A1 (en) | 2013-02-28 |
| WO2010131109A1 (en) | 2010-11-18 |
| EP2429488A1 (en) | 2012-03-21 |
| MX2011012121A (en) | 2011-12-14 |
| AP2011006030A0 (en) | 2011-12-31 |
| CN102791252A (en) | 2012-11-21 |
| CL2011002841A1 (en) | 2012-05-04 |
| BRPI1007665A2 (en) | 2017-06-27 |
| US20160278389A1 (en) | 2016-09-29 |
| JP2012526794A (en) | 2012-11-01 |
| EP2429488A4 (en) | 2013-10-09 |
| US20100291241A1 (en) | 2010-11-18 |
| CO6470891A2 (en) | 2012-06-29 |
| CA2761508A1 (en) | 2010-11-18 |
| AU2010247098A1 (en) | 2012-01-19 |
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