Hауково-Дослідний Інститут Тваринництва Лісостепу І Полісся Урср
Научно-исследовательский институт животноводства Лесостепи и Полесья УССР
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Micro-Organisms Or Cultivation Processes Thereof
(AREA)
Abstract
A method for the cryopreservation of embryos or ova comprises the filling of the cylindrical container with the following components: the medium for the elimination of cryoprotector, the first air bubble, the medium with cryoprotector containing embryo or ovum, the second first air bubble, the medium for the elimination of cryoprotector. Then the freezing at -30°C follows, and the container is placed into the liquid nitrogen. The ratio between the diameter of the first air bubble and the internal diameter of the container amounts to 2:1. The container is frozen in a vertical position, with the temperature of the cryoprotector containing embryo or ovum exceeding that of the medium for the elimination of cryoprotector at least by 15°C.
UA4855540A1990-07-301990-07-30Method for cryopreservation of embryos or ova
UA18452A
(en)