TWM578522U - Extraction system for lemon fermentation extract with antioxidation function - Google Patents

Abstract

This author is an extraction system for a lemon-fermented extract with antioxidant function. The lemon fermentation broth is formed by adding an extract system of lemon pure dew (including limonene) to the lemon fermentation broth, and is used to increase the antioxidant power. .

Description

Extraction system of lemon fermentation extract with antioxidant function

This creation is about an extraction system, especially an extraction system for lemon fermentation extracts with antioxidant properties.

Oxygen is the most abundant element on the earth, accounting for 53.8% of all elements and accounting for 21% of dry air. Most animals rely on inhaled oxygen to gain energy. The ground state triplet oxygen is the most stable oxygen molecule responsible for life. Although oxygen is absolutely required for the survival of almost all living things, oxygen is also fatal.

Stable oxygen molecules are converted into reactive oxygen species (ROS) by various physical, chemical and environmental factors, including enzyme systems, reducing metabolism, chemicals, pollutants and photochemical reactions. Highly reactive free radicals such as superoxide (O ₂ ^- ), hydroxy redical ( ‧ OH), peroxy radical (ROO‧) Hydrogen peroxide (H ₂ O ₂ ) and singlet oxygen (1O ₂ ).

Reactive oxygen species react very easily with cellular components (such as lipids, proteins, sugars, and nucleic acids, etc.) and cause damage to the human body, causing aging and brain diseases such as apoplexy and Parkinson's disease, and cardiovascular diseases. Such as heart disease, ischemia (ischemia) and arteriosclerosis, as well as various diseases such as dermal disease, inflammation, rheumatism and autoimmune diseases. Even cancer, for example, hydroxyl radicals destroy every part of the DNA, causing DNA strand breaks or base structures and causing permanent damage to the DNA. DNA is cut off, and during the repair process, DNA mutations may occur due to the inability to repair properly. This is currently a key factor in cell canceration.

Therefore, in the process of evolution, in order to use oxygen as a source of energy, and to prevent the active oxygen species from harming the cells, the organism develops an antioxidant defense mechanism to avoid or repair damage caused by free radicals. Can be broadly divided into enzyme and non-enzymatic antioxidant mechanisms.

The antioxidant mechanisms of enzymes are: (1) superoxide dismutase (SOD), the most important antioxidant enzyme system in the human body, and the superoxide (O ₂ ^- ) with higher activity. action into smaller active hydrogen peroxide _{(H 2 O 2); (} 2) catalase (catalase, CAT), CAT is the main role of H ₂ O ₂ produced SOD into harmless water and oxygen; (3) Valley Glutathione peroxidase (GPx), GPx mainly activates glutathione (GSH), directly reduces the lipid peroxide R-OOH to alcohol R-OH or decomposes H ₂ O ₂ .

However, the non-enzymatic antioxidant mechanisms are: (1) Vitamin C, which uses the molecular hydroxyl groups (OH) and free radicals, and OH releases an H ⁺ and an electron. When the free radicals get electrons, they become more stable compounds; (2) Vitamin E (Vitamin E), vitamin E has a phenolic structure, using the hydroxyl groups (OH) on it to release an H ⁺ and a Electrons, which make free radicals become more stable compounds; (3) β-carotene (β-carotene), β-carotene has two antioxidant capacities in the human body: First, it can break down with lipid peroxidation free radicals and interrupt lipids. Peroxidative chain reaction. Second, the absorption of oxygen due to light (for example, in the eyes) becomes excessive energy that excites oxygen to prevent oxidation.

In addition to the above, there are antioxidant substances such as glutathione, zinc, copper, selenium, iron, and the like. In the face of external factors such as aging, diet, living habits, diseases and environmental pollution, the imbalance between oxidation and antioxidant balance system will increase, and the antioxidant pressure will increase in the body, and the antioxidants will be increased by diet and health food. Ingestion to reinforce antioxidant defenses can reduce oxidative damage in living organisms.

To this end, the extraction system is improved to increase the extract (antioxidant) extracted from fruits and vegetables, and the extract extracted by the extraction system is further applied to increase the antioxidant function of the human body, which is desired by those skilled in the art. solved problem.

The main purpose of the present invention is to provide an extraction system for a lemon fermentation extract having an antioxidant function, which is formed by further extracting a lemon pure dew (containing limonene) extraction system from a lemon fermentation broth to form a lemon fermentation extract and used for Increase antioxidant capacity.

In order to achieve the above object, the present invention also discloses an extraction system for a lemon fermentation extract having an antioxidant function, comprising: a carrying device having an upper opening, the carrying device is equipped with a lemon product, and a heating process to produce a lemon vapor; a condensing device that communicates with the upper opening of the carrier device, and a condensation process of the lemon vapor to form a lemon pure dew, the lemon pure dew containing a limonene; A fermentation device comprising a lemon fermentation broth, the fermentation device connecting the condensing device through a pump, and adding the lemon pure dew to the lemon fermentation broth through the pump to form a lemon fermentation extract.

The present invention provides an embodiment in which an extraction system of a lemon fermentation extract having an antioxidant function is used, wherein the temperature of one of the support devices in the heating process is between 90 and 110 °C.

The present invention provides an embodiment of an extraction system for a lemon fermentation extract having an antioxidant function, wherein the condensation device has a temperature between -5 and 15 ° C in one of the condensation processes.

The present invention provides an embodiment of an extraction system of a lemon fermentation extract having an antioxidant function, wherein the lemon fermentation broth is fermented at room temperature.

In order to give your reviewers a better understanding and understanding of the characteristics of the creation and the effectiveness achieved, please follow the examples and supporting instructions to explain the following:

In view of the fact that external factors such as aging, diet, living habits, diseases and environmental pollution generate a large amount of free radicals and thus criticize, the antioxidant power is increased. Accordingly, the present invention proposes an extraction system of a lemon fermentation extract having an antioxidant function to solve the problems caused by the prior art.

In the following, the characteristics and the structure of the creation are further explained:

Please refer to FIG. 1 and FIG. 2 , which are respectively a flow chart of steps of an embodiment of the present invention and a schematic structural diagram of an embodiment of the present invention. As shown in Fig. 1, an extraction method of an antioxidant lemon fermentation extract comprises the steps of:

S1: taking a lemon product for heating process to produce lemon vapor;

S2: taking a lemon vapor to carry out a condensation process to form a lemon pure dew, and the lemon pure dew contains limonene;

S3: Add lemon pure dew to the lemon fermentation broth to form a lemon fermentation extract.

As shown in FIG. 2, one of the loading systems 1 of the present extraction system 1 is connected to a condensing device 20, and the condensing device 20 is connected to a fermentation device 40 through a pump 30.

And in step S1, a lemon product is placed in the carrying device 10, and the lemon product is subjected to a heating process to produce a lemon vapor, wherein the carrying device 10 has an upper opening through which the lemon vapor passes. Open the opening. The lemon product is a lemon peel or a lemon pulp or a combination thereof. Moreover, the temperature of one of the carrying devices 10 in the heating process is between 90 and 110 °C.

Next, as shown in step S2, the condensing device 20 is connected to the upper opening of the carrying device 10, and the lemon vapor is subjected to a condensation process to form a lemon pure dew. The lemon pure dew contains a limonene. The temperature of the condensing device 20 in the condensation process is between -5 and 15 °C.

Finally, as shown in step S3, the fermentation device 40 is connected to the condensation device 20 through the pump 30, and the lemon puree is added to the lemon fermentation liquid through the pump 30 (the lemon fermentation liquid is at room temperature) In the fermentation), a lemon fermentation extract is formed.

The lemon fermentation extract liquid phase adds more lemon pure dew (the limonene) than the conventional fermentation liquid to increase its antioxidant function.

Continued, continue to test the antioxidant function of the lemon fermentation extract of this creation.

There are many methods currently used to analyze antioxidant activity, including the removal of superoxide anion radical (O ₂ ^- ) produced by xanthine / xanthine oxidase, the ability to capture hydrogen peroxide (H2O2), and the removal of DPPH (α). , α-diphenylβ-pricrylhydrazyl) free radical ability determination, thiobarbituric acid (TBA) method and biochemiluminescence method to determine the ability to scavenge oxygen free radicals.

The method for analyzing the antioxidant activity of the present invention is: removing the DPPH (α, α-diphenylβ-pricrylhydrazyl) free radical ability, and the lipid generates free radicals during the self-oxidation process, resulting in lipid rancidity, free from antioxidants and DPPH. Based on the reaction formula (DPPH•+AH (antioxidant)→ DPPH+HA•), it is known that the antioxidant provides hydrogen to DPPH radicals when DPPH is removed, thereby inhibiting the oxidative chain reaction.

The lemon fermentation extract of the present invention is selected from a lemon fermentation broth having a concentration of 100%, 50%, 25%, 10%, and 1%, and 1%, 2%, and 3% of lemon pure dew is added. The reaction was carried out at room temperature (25 ° C) and three replicates were carried out using vitamin C as a standard (10000 ppm). The lemon fermentation extract was treated with four samples (1. Lemon fermentation extract (not sterilized), 2. Lemon fermentation extract supernatant (not sterilized), 3. Lemon fermentation extract lower layer (not sterilized), 4 . Lemon Fermentation Extract Browning (Sterilization)).

The experimental results are as follows:

Table 1. 100% of the lemon fermentation broth was added with DPPH antioxidant results of different concentrations (1%, 2%, 3%) of lemon pure dew.  Stock solution  1% lemon pure  2% lemon pure  3% lemon dew  Lemon Fermentation Extract (not sterilized)  85.1%  86.9%  88.1%  90.0%  Lemon fermentation extract supernatant (not sterilized)  89.2%  89.8%  91.5%  92.7%  Lemon fermentation extract lower layer (not sterilized)  83.7%  84.2%  86.0%  87.6%  Lemon Fermentation Extract Browning (Sterilization)  63.6%  63.8%  65.1%  66.8%  DPPH clearance - the ratio of free radicals in the sample to the DPPH reagent removal reagent (in %)  Table 1  

As can be seen from the above table, the upper layer of the lemon fermentation extract (not sterilized) has the highest antioxidant resistance in the case of adding 3% of lemon pure dew.

Therefore, this creation is a novelty, progressive and available for industrial use. It should be consistent with the patent application requirements of China's patent law. It is undoubtedly a new type of patent application, and the Prayer Council will grant patents as soon as possible.

However, the above descriptions are only preferred embodiments of the present invention, and are not intended to limit the scope of the present invention, and the variations, modifications, and modifications of the shapes, structures, features, and spirits described in the scope of the present patent application. , should be included in the scope of the patent application of this creation.

1‧‧‧ Extraction system

10‧‧‧ Carrying device

20‧‧‧Condensing device

30‧‧‧ pump

40‧‧‧ Fermentation unit

Figure 1 is a flow chart showing the steps of an embodiment of the present invention;

Fig. 2 is a schematic view showing the structure of an embodiment of the present invention.

Claims (4)

An extraction system for a lemon fermentation extract having an antioxidant function, comprising:  a carrier device having an upper opening, the carrier device being provided with a lemon product,  And performing a heating process to produce a lemon vapor;  a condensing device that communicates with the upper opening of the carrying device, and performs a condensation process on the lemon vapor to form a lemon pure dew, the lemon pure dew containing a limonene;  A fermentation device comprising a lemon fermentation broth, the fermentation device connecting the condensing device through a pump, and adding the lemon pure dew to the lemon fermentation broth through the pump to form a lemon fermentation extract.   The lemon fermentation extract having antioxidant function as described in claim 1 of the patent application scope  An extraction system, wherein the temperature of the carrier device in the heating process is between  Between 90~110 °C.   The lemon fermentation extract having antioxidant function as described in claim 1 of the patent application scope  An extraction system, wherein the temperature of the condensing device in the condensation process is between -5 and 15  Between °C.   The lemon fermentation extract having antioxidant function as described in claim 1 of the patent application scope  An extraction system wherein the lemon fermentation broth is fermented at room temperature.