M354070 八、新型說明： 【新型所屬之技術領域】 本創作係關於一種基板，特別是一種有關於生化檢測與免疫檢測所使用之流 體檢測試片所使用的基板。 【先前技術】 以流體檢測試片進行生化檢測與免疫檢測的習知技術中，流體檢測試片在其 _基板或底材上設計有流道或微流道結構，而因流道周圍並非吸水材質，且待測 拳流體多為含有如蛋白質或是醣類等黏滯度高之組成物，所以當待測流體流過 後，會在流道上殘留，使得待測流體無法完全反應，如此一來，不僅造成待測 流體的浪費，更可能造成最終測試結果的誤差。 此外s知技術的抓體檢測試片在流體傳送方面，可設計有微流道結構，並 係利用微流道結構產生的毛細現象，將流體經過流道被動傳送至反應侧區 域；另-種方式則是在注入待測流體時即利用加麼等方式，給予流體一驅動力， 使得流體可絲通碰道，到達反應勤旭域。但是無論是上雜―種方式， 待測流體注入流道後常常產生大小不一的氣泡使得流道阻塞，造成實際測量上 之誤差，甚至致使測試失敗。 最後，習知技術_測試片’在製作上多使用模鑄或射出成型或壓印(imprint) 的方式在基板上做出流道或織道結構，所以必縱用聚乙稀（pE)、聚氣乙 烯（PVC)或聚丙烯（PP)等價格較高之塑膠聚合物製作，且模具耗損較快， 進而造成試片之總體成本的提高。 ' ' 【新型内容】 為克服上述之缺點’本創作提供一種流體檢測試片之基板，基板上表面向 下凹設至少-流道，此流道包含依序連接之第一流體區、第二流體區與第三流 5 M354070 體區，第一流體區係供流體之注入。此基板之特徵在於第二流體區與第三流體 區之底部形成有硝化纖維層，此硝化纖維層包含有中空網狀構型，而流道中第 二流體區是供流體傳送之用，第三流體區是供流體反應之用，且第二流體區的 硝化纖維層平均厚度不大於第三流體區硝化纖維層厚度。 因此，本創作之主要目的，係提供一種流體檢測試片之基板，因具有可吸 水的硝化纖維層，故可避免流道之待測流體殘留。 本創作之另一目的，係提供一種流體檢測試片之基板，其具有可吸水的硝 化纖維層，由於單位體積的硝化纖維吸水量係為定值，故可經由設定基板上硝 I 化纖維層的體積，而提供流體的定量檢測。 本創作之又-目的，係提供一種流體檢測試片之基板，具有中空網狀構型 的确’化纖維層，由於趙流財空網狀構型時，流體巾的氣泡會被破壞，故可 消彌較大的氣泡，避免微流道技術中氣泡阻塞流道的狀況發生，進而影響定量 分析結果。 /曰 【實施方式】 由於本創作係揭露-種流體檢職片之基板，其巾所糊物理、化學原理及 合液塗布技術’已為相關技術領域具有通常知識者所能明瞭，故以下文中之說 明’不再作完整描述。科’以下文中賴照之圖式，係表達與本創作特徵有 關之示意，並未亦不需要依據實際情形完整繪製，合先敘明。 如第1A圖所示，為本創作第一較佳實施例之流體檢測試片的基板示 流體檢測試片的基板i自上表面1〇向下凹設有流道u，流道u包含树序連 接的第-流體區⑴、第二流體區112與第三流體區113。第一流體區⑴ 流體^注入，流體在注入第一流體區ln後，經由第二流體區ιΐ2的傳送，到 達第三流舰II3 ’流體巾的制成份在第三顏區113進行反應。又 的實施狀態中，基板1為生物相容(bioc〇mpatibleh 又 請參考第1B圖’為第1A圖中沿Μ連線之剖面圖。基板1的特徵在於， 6 M354070 在第二流體區112與第三流體區in之底部均形成有硝化纖維層1121與113l， 而硝化纖維層1121與U31的構型為中空網狀，此種具有多孔性的中空網狀結 構，可以吸收由第-流體區U1流入的流體，使得流體中的待測成份與存在於 硝化纖維層1131中的試劑進行反應。由於硝化纖維層1121與U31係為可吸水 材質，故可避免流體殘留於流道u中，且由於流體流經中空網狀構型的硝化纖 維層1121與1131時’流體中的氣泡會被破壞，故可避免氣泡阻塞流道。。此 外，第二流體區112的硝化纖維層1121平均厚度！^不大於第三流體區113硝 化纖維層1131厚度Db ’亦即Da會小於或等於Db。此外，為降低所需生物檢 鲁體之體積’第二流體區112與第三流體區113的寬度(如第丨八圖所 示）較佳至少為〇.3mm。 在製作上，硝化纖維層1121與1131的形成方式，係將硝化纖維溶液澆注 (casting)於第二流體區U2與第三流體區113的底部，經乾燥後，於第二流體 區112底部則會形成石肖化纖維層lm，而於第三流體區113喊部則形成石肖化 纖維層1131。為達較佳的液注效果，流道n之表面粗經度（Ra值）以介於3 微米至50微米之間為佳。 本創作所提供之基板卜由料健積的辄麟吸水量係為紐，故可由 欲吸收之待測流體的體積推算出對應的硝化纖維溶液的體積，之後再行洗注。 Φ如此可以固定檢測所需液體之體積量，並適用於微量檢測。 1 上述之第一較佳實施例係為具有三個流體區域的檢測試片基板，而根據本創 作之精神’尚可在流道中加設第四流體區，以供儲存流道中多餘之流體。以下 將就本創作之第二實施例具有四做體區的檢測試片基板進行描述。 睛參考第2A ®，為本創作第二實施例之流體檢測試片的基板示意圖。基 板2自上表面2G向下凹設有流道21，流道21包含有依序連接的第—流體區 'V體區212苐二流體213區與第四流體區214。第一流體區211係 供机體之注入，流體在注入第一流體區211後，經由第二流體區212的傳送， 到達第三流體區Μ，流體中的待測成份在第三流體區213反應。 7 M354070 請參考第2B圖，為第2A圖沿AA連線之剖面圖。在第二流體區212與第 三流體區213之底部均形成有硝化纖維層2121與2131 ’且第二流體區212的硝 化纖維層21U平均厚度Dc等於第三流體區213硝化纖維層2131厚度Dd。第 四流體區214之底部與第二及第三流體區212、213相同，亦形成有硝化纖維層 2M1，且亦為中空網狀構型，以供多餘流體之貯存。而第四流體區214底部之 硝化纖維層2141，在製作上，與第二及第三流體區212、213底部之硝化纖維層 2121與2131相同，均以硝化纖維溶液經洗注於第二流體區212、第三流體區213 與第四流體區214之底部再經乾燥後所形成。M354070 VIII. New description: [New technical field] This creation is about a substrate, especially a substrate used for the fluid test strips used in biochemical detection and immunoassay. [Prior Art] In the conventional technique of performing biochemical detection and immunodetection using a fluid detecting test piece, the fluid detecting test piece is designed with a flow path or a micro flow path structure on the substrate or the substrate, and the water around the flow path is not water absorbing. Material, and the punching fluid to be tested is mostly composed of a highly viscous composition such as protein or sugar. Therefore, when the fluid to be tested flows, it will remain on the flow path, so that the fluid to be tested cannot be completely reacted. Not only causes waste of the fluid to be tested, but is more likely to cause errors in the final test results. In addition, the grasping test piece of the known technology can be designed with a micro-channel structure in the fluid transfer, and utilizes the capillary phenomenon generated by the micro-channel structure to passively transfer the fluid through the flow path to the reaction side region; Then, when the fluid to be tested is injected, the driving force is given to the fluid, so that the fluid can pass through the tunnel and reach the reaction domain. However, no matter the hybrid mode, the fluid to be tested is often injected into the flow channel, which often causes bubbles of different sizes to block the flow channel, causing errors in actual measurement and even causing the test to fail. Finally, the prior art _ test piece 'uses molding or injection molding or imprinting on the substrate to make a flow path or a weave structure on the substrate, so it is necessary to use polyethylene (pE), Polyethylene (PVC) or polypropylene (PP) and other high-priced plastic polymers are produced, and the mold wears out quickly, which in turn leads to an increase in the overall cost of the test piece. ' ' [New content] In order to overcome the above disadvantages, the present invention provides a substrate for a fluid detecting test piece, wherein at least a flow path is recessed downwardly on the upper surface of the substrate, the flow path including the first fluid region sequentially connected, and the second The fluid zone and the third flow 5 M354070 body zone, the first fluid zone is for fluid injection. The substrate is characterized in that a nitrocellulose layer is formed at the bottom of the second fluid region and the third fluid region, the nitrocellulose layer comprises a hollow network configuration, and the second fluid region in the flow channel is for fluid transfer, and the third The fluid zone is for fluid reaction, and the average thickness of the nitrocellulose layer of the second fluid zone is no greater than the thickness of the nitrocellulose layer of the third fluid zone. Therefore, the main purpose of the present invention is to provide a substrate for a fluid detecting test piece which has a water-repellent nitrocellulose layer, thereby avoiding residual fluid to be tested in the flow path. Another object of the present invention is to provide a substrate for a fluid detecting test piece having a water absorbing nitrocellulose layer. Since the water volume per unit volume of nitrocellulose is constant, the nitriding fiber layer can be set on the substrate. The volume provides a quantitative detection of the fluid. The purpose of the present invention is to provide a substrate for a fluid detecting test piece, which has a hollow network configuration and a 'fibrous fiber layer. The air bubble of the fluid towel is destroyed due to the Zhaoliucai network configuration, so it can be eliminated. Larger bubbles prevent the occurrence of bubbles blocking the flow path in the microfluidic technology, which in turn affects the quantitative analysis results. /曰[Embodiment] As the author of this creation reveals the substrate of the fluid inspection film, the physics, chemical principle and liquid coating technology of the towel are well known to those of ordinary skill in the related art, so the following The description 'will not be fully described. The diagrams in the following texts are expressed in terms of the characteristics of this creation, and they are not required to be completely drawn according to the actual situation. As shown in FIG. 1A, the substrate i of the fluid detecting test piece of the fluid detecting test piece according to the first preferred embodiment of the present invention is provided with a flow path u from the upper surface 1〇, and the flow path u includes a tree. The first fluid region (1), the second fluid region 112, and the third fluid region 113 are sequentially connected. The first fluid zone (1) is fluidly injected, and after the fluid is injected into the first fluid zone ln, the second fluid zone 113 is reacted via the second fluid zone ι2 to the third zone 113. In another embodiment, the substrate 1 is biocompatible (see also FIG. 1B' is a cross-sectional view along the Μ line in FIG. 1A. The substrate 1 is characterized by 6 M354070 in the second fluid region 112. The nitrocellulose layers 1121 and 113l are formed on the bottom of the third fluid zone in, and the nitrocellulose layers 1121 and U31 are in the form of a hollow mesh. The porous hollow mesh structure can absorb the first fluid. The fluid flowing in the zone U1 causes the component to be tested in the fluid to react with the reagent present in the nitrocellulose layer 1131. Since the nitrocellulose layers 1121 and U31 are water-absorbent, fluid can be prevented from remaining in the flow channel u. Moreover, since the bubbles in the fluid are destroyed when the fluid flows through the nitrocellulose layers 1121 and 1131 of the hollow network configuration, the bubbles can be prevented from blocking the flow path. Further, the average thickness of the nitrocellulose layer 1121 of the second fluid region 112 ^^ is not greater than the third fluid region 113 nitrocellulose layer 1131 thickness Db 'i. That is, Da will be less than or equal to Db. In addition, in order to reduce the volume of the desired biopsy body 'the second fluid region 112 and the third fluid region 113 Width (such as Preferably, the figure 8 is at least 〇3 mm. In production, the nitrocellulose layers 1121 and 1131 are formed by casting a nitrocellulose solution to the bottom of the second fluid zone U2 and the third fluid zone 113. After drying, a stone-shadowed fiber layer lm is formed at the bottom of the second fluid region 112, and a stone-sharpened fiber layer 1131 is formed at the third fluid region 113. For better liquid injection effect, flow The surface roughness (Ra value) of the road n is preferably between 3 micrometers and 50 micrometers. The substrate provided by the creation of the substrate is a nucleus water absorption system, so it can be absorbed by the sample to be absorbed. The volume of the fluid is calculated by the volume of the corresponding nitrocellulose solution, and then washed. Φ can fix the volume of the liquid required for detection and is suitable for micro-detection. 1 The first preferred embodiment described above has three The test piece substrate is detected in the fluid area, and according to the spirit of the present invention, a fourth fluid area may be added to the flow path for storing excess fluid in the flow path. The second embodiment of the present invention has four Body test strip substrate The eye is referred to as the second substrate, and is a schematic view of the substrate of the fluid detecting test piece according to the second embodiment of the present invention. The substrate 2 is recessed from the upper surface 2G with a flow path 21, and the flow path 21 includes the sequentially connected first- The fluid region 'V body region 212 苐 two fluid 213 region and the fourth fluid region 214. The first fluid region 211 is for the injection of the body, and after the fluid is injected into the first fluid region 211, the second fluid region 212 is transferred. Upon reaching the third fluid zone, the component to be tested in the fluid reacts in the third fluid zone 213. 7 M354070 Please refer to Figure 2B for a cross-sectional view along line AA of Figure 2A. In the second fluid zone 212 and the third The bottom of the fluid zone 213 is formed with nitrocellulose layers 2121 and 2131 ' and the average thickness Dc of the nitrocellulose layer 21U of the second fluid zone 212 is equal to the thickness Dd of the third fluid zone 213 nitrocellulose layer 2131. The bottom of the fourth fluid zone 214 is identical to the second and third fluid zones 212, 213, and is also formed with a nitrocellulose layer 2M1 and also in a hollow mesh configuration for storage of excess fluid. The nitrocellulose layer 2141 at the bottom of the fourth fluid zone 214 is identical in fabrication to the nitrocellulose layers 2121 and 2131 at the bottom of the second and third fluid zones 212, 213, and is immersed in the second fluid with the nitrocellulose solution. The bottom of the zone 212, the third fluid zone 213 and the fourth fluid zone 214 are formed after drying.
此外’本實補巾第_流艇、第二流舰、第三流_之構造 '尺寸與相 互連接關係、基板之較佳材料、表面粗糙度、基板中硝化纖維層之構型、形成 方式，均與第-實施例相同，故在此不再重複贅述。 以上所述僅為本創作較佳實施例而已，並非用以限定本創作申請專利權利； 同時以上的描述對於熟之本技術領域之專門人士應可日膽與實施，因此其他未 請 脫離^創作所揭示之精神下所完等效改變或修飾，均應包含於下述之申 專利範圍。 τ 【圖式簡單說明】 第1Α圖’為本創作第一實施例流體檢測試片的基板之示意圖。 第1B圖，為本創作第一實施例流體檢測試片的基板剖面之示意圖 f 2A圖，為本創作第二實施例流體檢測試片的基板之示意圖。 第2B圖’為本創作第二實施例流體檢測試片的基板剖面之示意圖In addition, the size and interconnection relationship of the structure of the shovel, the second stream, the third stream, the preferred material of the substrate, the surface roughness, the configuration of the nitrocellulose layer in the substrate, and the formation method The same as the first embodiment, so the details are not repeated here. The above description is only for the preferred embodiment of the present invention, and is not intended to limit the patent right of the present invention; at the same time, the above description should be daring and implemented for those skilled in the art, so the others are not invited to leave the creation. Equivalent changes or modifications in the spirit of the disclosure are intended to be included in the scope of the claims below. τ [Simplified description of the drawings] Fig. 1 is a schematic view showing the substrate of the fluid detecting test piece of the first embodiment of the present invention. Fig. 1B is a schematic view showing the substrate cross section of the fluid detecting test piece of the first embodiment of the present invention, which is a schematic view of the substrate of the fluid detecting test piece of the second embodiment. 2B is a schematic view of a substrate cross section of the fluid detecting test piece of the second embodiment of the present invention.
10、20 11 ' 21 【主要元件符號說明】 基板 上表面 流道 8 M35407010,20 11 ' 21 [Description of main component symbols] Substrate Upper surface Flow path 8 M354070
第一流體區 第二流體區 第三流體區 第四流體區 硝化纖維層 硝化纖維層平均厚度 硝化纖維層厚度 硝化纖維層平均厚度 硝化纖維層厚度 第二流體區的寬度 第三流體區的寬度 111 ' 211 112 、 212 113 、 213 214 1121 > 13 > 2121 > 2131 ' 2141First fluid zone second fluid zone third fluid zone fourth fluid zone nitrocellulose layer nitrocellulose layer average thickness nitrocellulose layer thickness nitrocellulose layer average thickness nitrocellulose layer thickness second fluid zone width third fluid zone width 111 '211 112 , 212 113 , 213 214 1121 > 13 > 2121 > 2131 ' 2141
Da Db Dc Dd WaDa Db Dc Dd Wa
WbWb