TWI767323B - Biochip detection device and method thereof - Google Patents

Abstract

The invention discloses a biochip detection device and a method thereof, comprising a substrate, a reaction module and a rotation unit arranged from bottom to top. The rotating unit can rotate on the bonded substrate and the reaction module. The rotating unit has a plurality of dropping holes, which can respectively drop different liquids to be tested, which penetrate into the reaction module to form the area to be tested, and then drop the test droplets into the center around the area to be tested. It is adsorbed and flows to the area to be tested to form a reaction with each other, and can be dripped with cleaning solution to filter out impurities, so as to obtain more accurate final test results. The volume of the liquid to be tested can be detected by only a small amount, and multiple different liquids to be tested can be detected at the same time, and the detection liquid can be uniformly flowed to the liquid to be tested by peripheral diffusion for reaction, which can effectively improve the detection accuracy.

Description

Biochip detection device and method thereof

The present invention relates to a biochip testing device and method thereof, and more particularly to the technical field of simultaneously testing a variety of different testing data and improving the accuracy of testing results.

Press, immunochromatography technology has played a very important role in today's medical system. It can be used to qualitatively or quantitatively measure the changes of various biological indicators to provide information such as disease diagnosis or drug treatment for laboratory personnel or medical personnel.

At present, the vast majority of test strips on the market are qualitatively designed to allow the tester to know the test results by visual inspection. method”, but the test strip of the previous case can only detect a single item and cannot detect multiple different test items at the same time. Another example is the "multi-sample rapid detection test strip device (1)" of the Republic of China Patent No. M554567, although it can detect a variety of different detection items at the same time, but when the detection liquid needs to flow to the reaction areas of multiple liquids to be tested, the detection liquid is The lateral flow in a single direction, and then flows to each reaction zone in sequence to react with the liquid to be tested. This method will cause the capacity of the test liquid to flow to each reaction zone from more to less, because the distribution of the test liquid is different. Evenly, the test liquid received by the liquid to be tested is different, resulting in inspection error, and even the problem of inaccurate inspection. Therefore, how to simultaneously detect a variety of different detection items and improve the detection accuracy through innovative detection device design is an urgent problem to be solved.

In view of the problems of the above-mentioned conventional techniques, the object of the present invention is to provide a kind of simultaneous detection of multiple different detection data, and only a trace amount of the liquid to be detected can be reacted with the detection liquid, which can not only reduce the difficulty of collecting and detect and greatly increase. The detection sample uses the peripheral diffusion flow mode of the detection liquid to uniformly combine and react with all the liquids to be tested at the same time, which can effectively improve the accuracy of the detection results.

According to the purpose of the present invention, a biochip detection device is provided, which includes a substrate, a reaction module and a rotation unit. The reaction module is attached to the substrate, and the reaction module includes a reaction layer, a liquid absorption layer and a fixing layer stacked from bottom to top. The reaction layer is adjacent to the substrate, and the liquid-absorbing layer has a liquid-absorbing opening and a liquid-absorbing area located around the liquid-absorbing opening. The fixed layer has a reaction opening, and the reaction opening is in communication with the liquid absorption opening. The rotating unit is arranged on the reaction module, and the rotating unit has a main body, a pivot member located on the main body and a plurality of drip holes. The pivot element connects the reaction module and the base material, and the rotating unit uses the pivot element to rotate on the reaction module. The positions of the drop holes correspond to the reaction openings and are communicated with each other.

According to the above technical features, the sides of the dripping holes respectively have a different dripping mark.

According to the above technical features, the body has a drop-in area, and the drop-in holes are located in the drop-in area, and are arranged around the center of the drop-in area at intervals.

According to the above technical features, the body has a drop-in area, and the drop-in holes are located in the drop-in area and are staggered and spaced outward from the center of the drop-in area.

According to the above technical features, wherein the body has a dripping area, the dripping holes are located in the dripping area, and the dripping holes are arranged around at least two surrounding areas, which are divided into a first surrounding area and a first surrounding area. Two surrounding areas, the dripping holes located in the first surrounding area and the second surrounding area are staggered with each other.

According to the above technical features, the pore size of the reaction opening is larger than that of the liquid absorption opening, and the liquid absorption area is partially exposed to the reaction opening.

According to the above technical features, the size of the reaction layer is smaller than the size of the liquid absorption layer, and the reaction layer interferes with the liquid absorption opening and the local liquid absorption area.

According to the above technical features, the pivot member has a free rotating end and a fixed end, the fixed end is used for fixing the bottom surface of the base material, and the free rotating end is located on the body of the rotating unit.

According to the above technical features, wherein the base material has a first pivot hole, the fixed layer of the reaction module has a second pivot hole, the body of the rotating unit has a third pivot hole, and the pivot member is pivoted through. in the first pivot hole, the second pivot hole and the third pivot hole.

According to the above technical features, the detection solution contains reactive antibodies and colloidal gold.

According to the purpose of the present invention, a biochip detection device is provided, which includes a substrate, a reaction module and a rotation unit. The reaction module is attached to the substrate, and the reaction module includes a reaction layer, a liquid absorption layer and a fixing layer stacked from bottom to top. The reaction layer is adjacent to the substrate, and the liquid-absorbing layer has a liquid-absorbing opening and a liquid-absorbing area located around the liquid-absorbing opening. The fixed layer has a reaction opening, and the reaction opening is in communication with the liquid absorption opening. The rotating unit is arranged on the reaction module, and the rotating unit has a main body, a pivot member located on the main body and a plurality of drip holes. The pivot element connects the reaction module and the base material, and the rotating unit uses the pivot element to rotate on the reaction module. The positions of the drop holes correspond to the reaction openings and are communicated with each other. Wherein, each drop-in hole is for a drop to be tested and penetrated through the reaction opening, the liquid suction opening to the reaction layer to form an area to be measured. When the body is rotated away from the reaction module by the pivot member, the reaction opening and the suction opening are for a detection droplet to drop into the center around the area to be measured, and the detection solution is adsorbed and flowed to the area to be measured by the suction area. The test areas can form a reaction with each other and obtain the final test result.

According to the above technical features, the volume of the liquid to be tested is between 0.1 micrograms and 0.2 micrograms.

According to the above technical features, the rotating unit is a transparent substrate.

According to another object of the present invention, a biochip detection method is provided, which includes arranging a substrate, a reaction module, and a rotating unit from bottom to top, and the substrate and the reaction module are attached and fixed. A liquid to be tested is dropped into at least one of the plurality of dropping holes of the rotating unit, and the liquid to be tested penetrates the reaction module to form an area to be tested. The rotating rotating unit is away from the reaction module and the substrate. A detection liquid is dropped into the center around the area to be tested, so that the detection liquid and the liquid to be tested react with each other to generate an initial detection result. A cleaning solution is dropped into the area to be tested to filter out impurities in the initial test result, so as to obtain a final test result, such as a color test result.

According to the above technical features, after the step of dropping the liquid to be tested, a drying step is further included, and the drying time is between 5-15 minutes.

According to the above technical features, after the step of dropping the detection liquid, a drying step is further included, and the drying time is between 5-15 minutes.

According to the above technical features, the cleaning liquid is pure water.

According to the above technical features, the sides of the dripping holes respectively have a different dripping mark, and the dripping mark corresponds to the detection data of the liquid to be tested.

According to the above technical features, the testing data includes the data of the subject, the specimen and the testing items.

According to the above technical features, the rotating unit is a transparent substrate made of a transparent material.

In order to facilitate the examiners to understand the technical features, content and advantages of the present invention and the effects that can be achieved, the present invention is hereby described in detail with the accompanying drawings and in the form of embodiments as follows. The subject matter is only for illustration and auxiliary description, and is not necessarily the real scale and precise configuration after the implementation of the present invention. Therefore, the ratio and configuration relationship of the attached drawings should not be interpreted or limited to the scope of rights of the present invention in actual implementation. Together first to describe.

Please refer to Figure 1, Figure 2, Figure 3 and Figure 4 at the same time, Figure 1 is a schematic diagram of the structure of the biochip detection device of the present invention; Figure 2 is an exploded view of the structure of Figure 1; Figure 3 is FIG. 1 is a cross-sectional view of the structure along the section line AA'; FIG. 4 is a schematic view of the structure of the rotating unit according to the first embodiment of the present invention. The biochip detection device 100 of the present invention is applied to immunochromatography technology. First, the innovative structural design of the present invention is described. The biochip detection device 100 includes a substrate 10 , a reaction module 20 and a rotation unit 30 . The reaction module 20 is attached to the substrate 10 , and the rotating unit 30 is disposed on the reaction module 20 . The reaction module 20 includes a reaction layer 21 , a liquid absorption layer 22 and a fixed layer 23 stacked from bottom to top. The reaction layer 21 is adjacent to the substrate 10 , and the fixed layer 23 is adjacent to the rotating unit 30 . The liquid-absorbing layer 22 has a liquid-absorbing opening 221 and a liquid-absorbing area 222 located around the liquid-absorbing opening 221 .

The diameter of the reaction opening 231 is larger than that of the liquid suction opening 221 , and the liquid suction area 222 is partially exposed to the reaction opening 231 , which is helpful for the inspector to directly observe the reaction process and state of the detection. The size of the reaction layer 21 is smaller than the size of the liquid absorption layer 22, and the reaction layer 21 interferes with the liquid absorption opening 221 and the partial liquid absorption area 222. As long as the reaction layer 21 can cover the range of the liquid absorption opening 221, the liquid ( For example, the liquid to be tested, detection liquid, cleaning liquid, etc. can fall into the reaction layer 21. The reaction layer 21 can be a nitro-cellulose membrane, a PVDF membrane (polyvinylidone (PVDF) membrane), a nylon membrane (nylon) membrane), etc., are used to adsorb the protein to be detected in the liquid 40 to be detected.

The rotating unit 30 has a main body 31 , a pivot member 32 located on the main body 31 , and a plurality of drip holes 33 . The rotating unit 30 is a transparent substrate. The pivot member 32 connects the reaction module 20 and the substrate 10 . The rotation unit 30 uses the pivot member 32 to rotate on the reaction module 20 . The positions of the drop holes 33 correspond to the reaction openings 231 and communicate with each other. The pivot member 32 has a free rotating end 321 and a fixed end 322. The fixed end 322 is used to fix the bottom surface of the substrate 10, and the free rotating end 321 is located on the body 31 of the rotating unit 30, so that the body 31 can be assembled The material 10 and the reaction module 20 are rotated horizontally, and the rotation angle is not limited, and can be operated according to the needs of the inspector. Specifically, the substrate 10 has a first pivot hole 11 , the fixed layer 23 of the reaction module 20 has a second pivot hole 232 , and the body 31 of the rotating unit 30 has a third pivot hole 35 . The pivot member 32 is pivotally connected through the first pivot hole 11 , the second pivot hole 232 and the third pivot hole 35 , so that one side of the substrate 10 , the reaction module 20 and the rotating unit 30 can face each other. connect.

It is worth noting that the body 31 has a dripping area F, and the dripping holes 33 are located in the dripping area F, and are arranged around the center of the dripping area F at intervals. Alternatively, the dripping holes 33 are arranged at a staggered interval from the center of the dripping region F outward. As shown in FIG. 4 , in this embodiment, the dripping area F has four dripping holes 33 , which are arranged around each other at intervals, and the optimal design is that the four dripping holes 33 are equidistant. In order to detect different detection data at the same time, the sides of the drip holes 33 respectively have a different drip mark 34, such as a number number, a pattern or an English letter, etc. Add the type of limited drip mark 34. The drip mark 34 is a test data corresponding to the liquid to be tested 40 , and the test data includes relevant data such as the subject's data, the specimen, and the test items. In this way, the tester can clearly identify each object by the drip mark 34 . Corresponding inspection data can effectively prevent inspectors from negligent work.

Please also refer to FIG. 5 , which is a structural state diagram of the present invention when the liquid to be tested is dropped. Each dropping hole 33 is for dropping a liquid to be tested 40. The liquid to be tested 40 may be body fluid or blood. Here, four dropping holes 33 are used as an example to illustrate. The test liquids 40 are all different and belong to different test data, wherein the test subject data and the specimen are different, but the test items can be the same or different, and can be adjusted appropriately according to the test requirements.

As shown in FIG. 6 , it is a structural state diagram of the present invention after the rotating unit is rotated. Since the positions of the dripping holes 33 correspond to the reaction openings 231 and are connected to each other, after the liquid to be tested 40 is dripped from the dripping holes 33 , the liquid to be tested 40 will penetrate through the reaction openings 231 and the liquid suction openings 221 to the reaction layer 21 to form an area to be tested. At this time, the rotating unit 30 can be operated. The main body 31 is rotated horizontally by an angle through the free rotating end 321 of the pivot member 32 to keep the rotating unit 30 away from the reaction module 20 . At this time, after dropping four liquids to be tested 40 (such as the numbers 1, 2, 3, and 4 represented by the drip marks 34 ), the inspector can directly view the four regions to be tested formed on the reaction layer 21 , such as regions A1, A2, A3, A4.

Next, please refer to FIG. 7 , which is a structural state diagram of the present invention when the liquid on the detection side is dripped. The reaction opening 231 and the suction opening 221 are for a detection solution 50 to drop into the center of the surrounding areas A1, A2, A3, and A4, and the detection solution 50 is adsorbed and diffused to the area A1 by the suction area 222. , A2, A3, A4 to form a reaction with each other. Among them, the liquid suction area 222 is located around the liquid suction opening 221, so the detection liquid 50 is subjected to the capillary action of the liquid suction area 222, and the detection liquid 50 flows uniformly and simultaneously toward the areas A1, A2, A3, and A4 at a free angle until the liquid is absorbed. The area 222 enables all areas A1 , A2 , A3 , and A4 to receive the detection liquid 50 uniformly, so that the detection liquid 50 and the to-be-tested liquid 40 of the detection liquid 50 are combined and reacted. It is worth noting that the capacity of the liquid to be tested 40 is between 0.1 micrograms and 0.2 micrograms, and the present invention only needs a small amount of the liquid to be tested 40 to perform subsequent detection reactions, which is compared with the conventional detection test pieces. Only when the liquid volume is as high as 1 mg or more can be detected, the present invention can indeed reduce the difficulty of collecting and testing and greatly increase the efficiency of testing samples.

The detection solution 50 includes a mixture of reactive antibody and colloidal gold, wherein the colloidal gold and the reactive antibody are combined in advance and the region where the reactive antibody can bind to the protein to be detected is reserved. Colloidal gold has been widely used in biomedical research fields, especially in medical testing. Colloidal gold is essentially a coating process in which proteins and other macromolecules are adsorbed to the surface of colloidal gold particles, that is, the negative charge on the surface of colloidal gold particles and proteins, such as Staphylococcus protein A, immunoglobulins, toxins, glycoproteins, enzymes, Non-covalent positively charged groups such as antibiotics and hormones form strong bonds due to electrostatic adsorption. Due to capillary action, the detection liquid 50 moves along the direction of the adsorbent liquid absorption area 222 , and when it moves to the areas A1 , A2 , A3 , and A4 , the protein to be detected in the detection liquid 50 will react with the liquid to be tested 40 . Antibodies produce specific binding reactions between antibodies and antigens.

As shown in FIG. 8 and FIG. 9, it is a structural state diagram of the present invention in which the cleaning solution is dripped and a structural state diagram of the present invention showing the reaction detection result. As described in the previous paragraph, the detection solution 50 and the test solution 40 in the regions A1, A2, A3, and A4 carry out the specific binding reaction of the antibody and the antigen, and the phenomenon of the binding reaction is called a positive reaction, that is, there will be more The antibody of the colloidal gold undergoes a binding reaction with the protein to be detected in the detection solution 50; otherwise, the phenomenon of no binding reaction is called a negative reaction, that is, there will be no specific binding reaction between the antibody and the antigen. In actual implementation, the detection liquid 50 can be adsorbed by the liquid suction region 222 and diffused and flowed to the regions A1, A2, A3, and A4 to form a reaction with each other, so that the liquid of the detection liquid 50 can be uniformly dispersed without being concentrated. At a certain point, the detection accuracy can be further greatly improved.

As shown in FIG. 9 and FIG. 10, it is a structural state diagram of the present invention in which the cleaning solution is dripped and another structural state diagram of the present invention showing the reaction detection result. The cleaning solution 60 is dropped into the center around the areas A1, A2, A3, and A4, and the cleaning solution 60 is adsorbed by the suction area 222 and diffused toward the surrounding area to flow to the areas A1, A2, A3, and A4. The detection solution 50 in A3 and A4 that has not undergone the specific binding reaction of antibody and antigen with the solution to be tested 40 is filtered out, which helps to improve the detection accuracy.

As shown in Fig. 9, the regions A1, A2 and A3 show more significant color performance than other regions due to the aggregation of a large amount of colloidal gold. As shown in Fig. 10, after dripping the cleaning solution 60, only Leaving the area A2 is where the color expression appears. It can be seen that, before the cleaning solution 60 is not used, the areas A1 and A3 belong to false positive phenomena. Therefore, the use of the cleaning liquid 60 in the present invention can indeed make the final detection result more accurate.

In addition to the above-mentioned first embodiment using the structure design of four dropping holes 33 , as shown in FIG. 11 , it is another structural state diagram showing the reaction detection result of the present invention. The body 31 has a dripping area F, and the dripping holes 33 are located in the dripping area F, and are arranged around the center of the dripping area F at intervals. Since the capacity of the liquid to be tested 40 is between 0.1 micrograms and 0.2 micrograms, only a very small amount can be detected, so the pore size of the dripping holes 33 can be designed to be smaller, and the number of dripping holes 33 can be limited in the detection space. to the maximum quantification. Also as shown in FIG. 12 , it is another structural state diagram showing the reaction detection result of the present invention. The dripping holes 33 are located in the dripping area F, and the dripping holes 33 are arranged around at least two surrounding areas, which are divided into a first surrounding area S1 and a second surrounding area S2. The first surrounding area S1 is adjacent to the center of the dripping area F, the second surrounding area S2 is far from the center of the dripping area F, and the dripping holes 33 located in the first surrounding area S1 and the second surrounding area S2 are staggered with each other. . The drop-in hole 33 is designed in a staggered arrangement, so that when the detection liquid 50 is dropped into the center around the multiple test areas, the test liquid 50 is absorbed by the liquid suction area 222 and diffused toward the surrounding area to flow to all the test areas to avoid each other. By forming the reaction, the detection liquid 50 can flow to all the regions to be tested to achieve uniformity, and the detection result will not be affected due to the large increase in the number of the dripping holes 33 .

Please refer to Fig. 2, Fig. 5, Fig. 7, Fig. 9 and Fig. 13 at the same time, and Fig. 13 is a flow chart of the steps of the present invention. In step B1 , a substrate 10 , a reaction module 20 and a rotating unit 30 are arranged from bottom to top, and the substrate 10 and the reaction module 20 are attached and fixed. In step B2, a liquid to be tested 40 is dropped into at least one of the plurality of instillation holes 33 of the rotating unit 30, and the liquid to be tested 40 penetrates the reaction module 20 to form an area to be tested. After the step of dropping the liquid to be tested 40, a drying step (not shown in the figure) is further included, and the drying time is 5-15 minutes, and the drying time is used as the reaction time for forming the area to be tested. In step B3 again, the rotating unit 30 is rotated away from the reaction module 20 and the substrate 10 . In step B4, a detection liquid 50 is dropped into the center around the area to be tested, so that the detection liquid 50 and the liquid to be tested 40 react with each other to generate an initial detection result. Wherein, after the step of dropping the detection liquid 50, a drying step (not shown in the figure) is further included, and the drying time is between 5-15 minutes. Reaction time. The detection solution 50 contains the reactive antibody and colloidal gold, wherein the colloidal gold and the reactive antibody are combined in advance and retain the region where the reactive antibody can bind to the protein to be detected. Since the above-mentioned embodiment has already described the composition and function of the detection solution 50 in detail. , so it is not repeated here. In step B5, a cleaning solution 60 is dropped into the area to be tested. The cleaning solution 60 is pure water. The cleaning solution 60 is used to filter out impurities in the initial detection result, thereby obtaining a final detection result.

Looking at the above, it can be seen that the present invention breaks through the previous technology, and the innovative improvement and detection method of the structure can detect a variety of different detection data at the same time. The difficulty of detection and greatly increase the detection sample, and the peripheral diffusion flow of the detection liquid is used to uniformly combine and react with all the liquids to be tested at the same time. In addition, the cleaning liquid is used to improve the accuracy of the detection results, which can effectively improve the overall detection. The accuracy of the results has indeed achieved the desired improvement effect, and it is not easy for those who are familiar with the technology to think. Furthermore, the present invention has not been disclosed before the application, and its progressiveness and practicability are obvious. If you meet the application requirements for a patent, you can file a patent application in accordance with the law. I urge your bureau to approve this patent application for invention, in order to encourage invention.

The above-mentioned embodiments are only to illustrate the technical ideas and characteristics of the present invention, and the purpose is to enable those who are familiar with the art to understand the content of the present invention and implement it accordingly. It should not be used to limit the patent scope of the present invention. That is, all equivalent changes or modifications made according to the spirit disclosed in the present invention should still be covered within the patent scope of the present invention.

100: Biochip detection device 10: Substrate 11: The first pivot hole 20: React Mods 21: Reactive Layer 22: Absorbent layer 221: suction opening 222: suction area 23: Fixed layer 231: reaction opening 232: Second pivot hole 30: Rotary unit 31: Ontology 32: Pivot piece 321: Rotating free end 322: Fixed end 33: Drip hole 34: Drip markers 35: The third pivot hole 40: liquid to be tested 50: detection liquid 60: Cleaning solution F: dripping area A1, A2, A3, A4: Area S1: First Surrounding Area S2: Second Surround Area B1, B2, B3, B4, B5: Steps

Fig. 1 is a schematic diagram of the structure of the biochip detection device of the present invention. Figure 2 is an exploded view of the structure of Figure 1. Fig. 3 is a sectional view of the structure along the line A-A' of Fig. 1. FIG. 4 is a schematic structural diagram of the first embodiment of the rotating unit of the present invention. Fig. 5 is a structural state diagram of the present invention when the liquid to be tested is dropped. Fig. 6 is a structural state diagram of the present invention after the rotating unit is rotated. Fig. 7 is a structural state diagram of the present invention when the liquid is dripped into the detection side. Fig. 8 is a structural state diagram showing the reaction detection result of the present invention. Fig. 9 is a structural state diagram of the present invention in which the cleaning solution is dripped. Fig. 10 is another structural state diagram showing the reaction detection result of the present invention. FIG. 11 is a schematic structural diagram of the second embodiment of the rotating unit of the present invention. Fig. 12 is a schematic structural diagram of the third embodiment of the rotating unit of the present invention. Fig. 13 is a flow chart of the steps of the present invention.

100: Biochip detection device

10: Substrate

11: The first pivot hole

21: Reactive Layer

22: Absorbent layer

221: suction opening

222: suction area

23: Fixed layer

231: reaction opening

232: Second pivot hole

30: Rotary unit

31: Ontology

32: Pivot piece

321: Rotating free end

322: Fixed end

33: Drip hole

35: The third pivot hole

Claims (22)

A biological chip detection device, comprising: a substrate (10); a reaction module (20), which is attached to the substrate (10), the reaction module (20) including a reaction module stacked from bottom to top layer (21), a liquid absorption layer (22) and a fixed layer (23), the reaction layer (21) is adjacent to the substrate (10), the liquid absorption layer (22) has a liquid absorption opening (221) With a liquid suction area (222) around the liquid suction opening (221), the fixed layer (23) has a reaction opening (231), the reaction opening (231) and the liquid suction opening (221) ) to form a communication; and a rotating unit (30) arranged on the reaction module (20), the rotating unit (30) having a body (31), a pivot member (31) on the body (31) 32) and a plurality of dropping holes (33), the pivot member (32) connects the reaction module (20) and the base material (10), the rotation unit (30) uses the pivot member (32) to The reaction module (20) rotates upward, and the positions of the dripping holes (33) correspond to the reaction openings (231), and are connected to each other with the reaction layer (21). The biochip detection device according to claim 1, wherein the sides of the dripping holes (33) are respectively provided with a different dripping mark (34). The biochip detection device as claimed in claim 1, wherein the body (31) has a drop-in area (F), the drop-in holes (33) are located in the drop-in area (F), and the drop-in area (F) The center position of the area (F) is arranged around the center at intervals. The biochip detection device as claimed in claim 1, wherein the body (31) has a drop-in area (F), the drop-in holes (33) are located in the drop-in area (F), and the drop-in area (F) The central position of the area (F) is arranged around the outside at intervals, and the dripping holes (33) are staggered and arranged at intervals. The biochip detection device according to claim 1, wherein the body (31) has a drop-in area (F), the drop-in holes (33) are located in the drop-in area (F), and the drop-in holes (33) The system surround is set to At least two surrounding areas, divided into a first surrounding area (S1) and a second surrounding area (S2), the drip holes located in the first surrounding area (S1) and the second surrounding area (S2) (33) are staggered with each other. The biochip detection device according to claim 1, wherein the diameter of the reaction opening (231) is larger than the diameter of the liquid suction opening (221), and the liquid suction region (222) is partially exposed to the reaction opening ( 231). The biochip inspection device according to claim 1, wherein the size of the reaction layer (21) is smaller than the size of the liquid absorption layer (22), and the reaction layer (21) interferes with the liquid absorption opening (221) and partially the liquid absorption layer (221). Suction area (222). The biochip detection device according to claim 1, wherein the pivot member (32) has a free rotating end (321) and a fixed end (322), and the fixed end (322) is used to fix the substrate (10). ), the free rotating end (321) is located on the main body (31) of the rotating unit (30). The biochip detection device according to claim 1, wherein the substrate (10) has a first pivot hole (11), and the fixed layer (23) of the reaction module (20) has a second pivot hole A hole (232), the body (31) of the rotating unit (30) has a third pivot hole (35), the pivot member (32) is pivoted through the first pivot hole (11), The second pivot hole (232) and the third pivot hole (35). A biological chip detection device, comprising: a substrate (10); a reaction module (20), which is attached to the substrate (10), the reaction module (20) including a reaction module stacked from bottom to top layer (21), a liquid absorption layer (22) and a fixed layer (23), the reaction layer (21) is adjacent to the substrate (10), the liquid absorption layer (22) has a liquid absorption opening (221) With a liquid suction area (222) around the liquid suction opening (221), the fixed layer (23) has a reaction opening (231), the reaction opening (231) and the liquid suction opening (221) ) to form a communication; and a rotating unit (30) arranged on the reaction module (20), the rotating unit (30) having a body (31), a pivot member (31) on the body (31) 32) and a plurality of drip holes (33), the pivot (32) connecting the reaction module (20) and the base material (10), the rotating unit (30) uses the pivot member (32) to rotate on the reaction module (20), the dripping holes The positions of (33) correspond to the reaction openings (231), and are communicated with each other; wherein, the body (31) has a drop-in area (F), and the drop-in holes (33) are located in the drop-in area (F). ), and the center position of the drop region (F) is arranged in a spaced manner outward, and each drop hole (33) is for a liquid to be tested (40) to be dropped and penetrated through the reaction opening (33). 231), the liquid suction opening (221) to the reaction layer (21) to form an area to be measured, when the main body (31) is rotated away from the reaction module (20) by the pivot member (32), The reaction opening (231) and the liquid suction opening (221) are for dripping a detection solution (50) to a central position around the area to be measured, and the detection solution (50) is dripped through the suction area (222). 50) Adsorption diffuses toward the surrounding area and flows to the area to be tested to form a reaction with each other, and obtain the final detection result. The biochip detection device according to claim 10, wherein the detection solution (50) contains reactive antibodies and colloidal gold. The biochip testing device according to claim 10, wherein the volume of the liquid to be tested (40) ranges from 0.1 microgram to 0.2 microgram. A biological chip detection method, comprising: arranging a substrate (10), a reaction module (20) and a rotation unit (30) from bottom to top, and the substrate (10) and the reaction module (20) In order to fit and fix; in at least one of the plurality of dripping holes (33) of the rotating unit (30), a liquid to be tested (40) is dropped into the dripping hole (33), and the liquid to be tested (40) Infiltrate the reaction module (20) to form an area to be tested, wherein the rotating unit (30) has a dripping area (F), and the dripping holes (33) are located in the dripping area (F), And the center position of the drop-in area (F) is arranged in an outer circle at intervals; the rotating unit (30) is rotated away from the reaction module (20) and the base material (10); around the center of the area to be tested A detection liquid (50) is dropped into the position, so that the detection liquid (50) and the liquid to be tested (40) react with each other to generate an initial detection result; and A cleaning solution (60) is dropped into the area to be tested, and the impurities in the initial test result are filtered out, so as to obtain a final test result. The biochip detection method according to claim 13, further comprising a drying step after the step of dropping the liquid to be tested (40), and the drying time is between 5-15 minutes. The biochip detection method according to claim 13, further comprising a drying step after the step of dropping the detection liquid (50), and the drying time is between 5-15 minutes. The biochip detection method according to claim 13, wherein the detection solution (50) comprises reactive antibodies and colloidal gold. The biological wafer detection method according to claim 13, wherein the cleaning liquid (60) is pure water. The biochip detection method according to claim 13, wherein the volume of the liquid to be tested (40) is between 0.1 micrograms and 0.2 micrograms. The biochip detection method according to claim 13, wherein the sides of the dripping holes (33) are respectively provided with a different dripping mark (34), and the dripping mark (34) corresponds to the liquid to be tested ( 40) of a test data. The biochip detection method as claimed in claim 19, wherein the detection data includes subject information, specimens and detection items. The biochip detection method according to claim 13, wherein the rotating unit (30) uses a pivot member (32) to rotate away from the reaction module (20) and the substrate (10). The biochip detection method according to claim 13, wherein the rotating unit (30) is a transparent substrate made of a transparent material.

Images