TWI714821B - Method and device for detecting biogenic amine of food materials - Google Patents

Abstract

A method includes: providing a biogenic amine sensor member, including a biogenic amine vapor absorbent substrate for absorbing biogenic amine moisture or vapor; disposing a biogenic amine sensitive material on the biogenic amine vapor absorbent substrate; combing the biogenic amine sensor member with a package or a food material; the biogenic amine sensitive material generating color change while the biogenic amine sensor member contacting with biogenic amine vapor or vapor.

Description

Method and device for detecting food biogenic amine

The present invention relates to a method and device for detecting biogenic amine in food materials; in particular, it relates to a non-contact method and device for detecting moisture or vapor in food materials; A method and device for detecting bio-amine vaporization of food materials with bio-amine sensitive materials.

For example, the conventional technology for detecting biological amines, such as the invention patent application of "A method for rapid detection of biological amines" in Chinese Patent Publication No. CN-102650628, discloses a method for rapid detection of biological amines. The method for rapid detection of biogenic amines includes the steps of: derivatizing a biogenic amine standard and a sample to be tested with a derivatizing agent, then carrying a chromophoric group, and spotting a sample on a chromatographic material polyamide film , And then use a spreading agent to expand, when the front of the spreading agent is 0.5 to 1 cm from the top of a film, stop the chromatography and produce color, and then observe the color development result.

In conclusion, the aforementioned No. CN-102650628 adopts a simple and rapid analysis method, which uses thin layer chromatography (TLC) for determination. When compared with other analysis methods, it does not require the use of expensive analytical instruments and reduces detection. Cost and rapid detection advantages, but its disadvantage is that the biological amine contained in the food still needs to be extracted and derivatized before detection and determination can be performed.

Another conventional technique for detecting biogenic amines, such as the invention patent application of "A method for rapid determination of biogenic amines in food" of Chinese Patent Publication No. CN-103149317, which discloses a method for rapid determination of biogenic amines in food law. The method for rapidly determining biogenic amines in food includes the steps: 1. Extract biogenic amines from a food sample; 2. Purify a biogenic amine extract; 3. Derivatize the biogenic amine extract; and 4. Use A developer is optimized and analyzed by thin layer chromatography.

In conclusion, the aforementioned No. CN-103149317 also adopts a simple and rapid analysis method, which also uses thin layer chromatography (TLC) for determination. When compared with other analysis methods, it does not require expensive analytical instruments, It has the advantages of reducing detection costs and rapid detection, but its disadvantage is that the biological amine contained in the food still needs to be extracted and derivatized before detection and determination can be performed.

Another conventional technique for detecting biogenic amines, such as the Chinese Patent Publication No. CN-104897897, "A method for detecting histamine using an immunological biosensor" invention patent application, which discloses a method for rapid determination of biogenic amines in food includes steps: S1 , Prepare an immunological biosensor, and detect the working curve of the sensor for use; S2, prepare a set of amine monoclonal antibodies; S3, prepare an enzyme-labeled antibody; S4, use the sensor to detect histamine. After mixing an equal volume of a sample solution to be tested and the enzyme-labeled antibody prepared in step S3, drip-coat an electrode working area, immerse the electrode in a test solution, and add hydrogen peroxide to pass the front and back peaks The change in the current value quantitatively determines the histamine concentration.

In conclusion, the aforementioned No. CN-104897897 uses a biosensor to detect biogenic amines, which has the advantages of low detection limit, high sensitivity, wide linear range, and suitable for on-site detection. However, the existing technology of the biosensor method for detecting biogenic amines is still not very mature, there are few reliable complete sets of equipment in the market, and its price is expensive.

Another conventional technique for detecting biogenic amines, such as the invention patent of "Portable Meat Freshness Detector" in the Republic of China Patent Publication No. TW-I467171, discloses a portable meat freshness detector. The portable meat freshness detector uses a sensing unit to measure an impedance value and a capacitive reactance value of the meat product. Then the measured impedance value and capacitive reactance value are calculated by an iterative algorithm set in a processing unit to obtain the index parameter value; then, the index parameter value and the memory are recorded The standard parameter values are compared to obtain the calculation result of the freshness of the meat, and the calculation result is displayed on a display unit.

In addition, the aforementioned No. TW-I467171 calculates the impedance value and capacitive reactance value of the meat surface by contacting the measurement, and obtains the freshness of the meat technology, although it has no need to damage the meat, rapid detection and convenient operation, etc. Advantages, however, contact measurement cannot be performed for packaged foods, so the freshness of packaged foods or ingredients cannot be confirmed.

Another conventional technique for detecting biological amines, such as the Chinese Patent Publication No. CN-104148626, "Aromatic Hydrocarbon Functionalized Gold Nanoparticles and Its Preparation Method" invention patent application, which discloses a cycloaromatic functionalized gold nanoparticle and its preparation method . A gold nanoparticle modifies an aromatic hydrocarbon CEC4 to the surface of the gold nanoparticle with a gold-thiol bond, and the concentration ratio of the aromatic hydrocarbon CEC4 to the gold nanoparticle is 2000000:23, and the particle diameter of the gold nanoparticle is 13nm And CEC4 functionalized gold nanoparticles have specificity in the detection of biogenic amines.

In addition, the aforementioned No. CN-104148626 utilizes the color change produced by the reaction of a compound with a biogenic amine to detect the freshness of food. Although this method has the advantages of simplicity and speed in discrimination, it still needs to be contacted and reacted with the extraction liquid, which is a contact or semi-contact measurement method. For the packaged food, contact measurement cannot be performed, so the freshness of the packaged food or ingredients cannot be confirmed.

Another conventional technique for detecting biogenic amines, such as the invention patent of "Polymeric food spoilage sensor" in US Patent Publication No. US-6593142, discloses a spoilage detector for food. The food spoilage (freshness) detector contains a polymer (polymer), and the polymer contains a composite material (polyaza macrocyclic transition metal) Complex], it comes into contact with spoiled food microorganisms [microorganisms] to release biogenic amines and react with biogenic amines to produce color changes [color change].

In addition, the aforementioned US-6593142 also uses the color change produced by the reaction of the compound with the biogenic amine to detect the freshness of the food. Although this method has the advantages of simplicity and speed in discrimination, it still needs to be contacted and reacted with the extraction liquid, which is a contact or semi-contact measurement method. For the packaged food, contact measurement cannot be performed, so the freshness of the packaged food or ingredients cannot be confirmed.

However, the aforementioned Chinese Patent Publication No. CN-102650628, Chinese Patent Publication No. CN-103149317, Chinese Patent Publication No. CN-104897897, Republic of China Patent Publication No. TW-I467171, Chinese Patent Publication No. CN-104148626 and the United States The detection device and method of Patent Publication No. US-6593142 must be further improved.

The aforementioned Chinese Patent Publication No. CN-102650628, China Patent Publication No. CN-103149317, China Patent Publication No. CN-104897897, Republic of China Patent Publication No. TW-I467171, China Patent Publication No. CN-104148626 and U.S. Patent Publication No. US-6593142 is only a reference for the technical background of the present invention and an explanation of the current state of technology development, and it is not intended to limit the scope of the present invention.

In view of this, in order to meet the above-mentioned technical problems and needs, the present invention provides a method and device for detecting biogenic amines in foodstuffs, which disposes a bioamine sensitive material on a bioamine gas adsorption substrate to form a bioamine detection Element, and use the bio-amine detection element to be combined with a package of food to be tested or a food to be tested, once the bio-amine detection element contacts a food bio-amine vaporized gas or a bio-amine water vapor, the bio-amine is sensitive The production of materials shows a bio-amine index to improve the technical shortcomings of the conventional methods and devices for detecting bio-amines in food materials.

The main purpose of the present invention is to provide a method and device for detecting biogenic amines of foodstuffs, which dispose a bio-amine sensitive material on a bio-amine gas adsorption substrate to form a bio-amine detection element, and use the bio-amine detection element Combined with a package of food to be tested or a food to be tested, once the bio-amine detection element contacts a bio-amine vaporized gas of a food material or a bio-amine water vapor, the bio-amine sensitive material is immediately used to generate a bio-amine index to display a bio-amine index. Achieve the purpose of simplifying the detection of food biogenic amines, accelerating the detection efficiency and reducing the cost of detection.

In order to achieve the above objective, the device for detecting bio-amine for food materials in a preferred embodiment of the present invention includes: a bio-amine gas adsorption substrate having a predetermined adsorption zone, and the predetermined adsorption zone of the bio-amine gas adsorption substrate is used to adsorb a Bio-amine vaporization gas or a bio-amine water vapor; a bio-amine sensitive material arranged on the bio-amine gas adsorption substrate to form a bio-amine detection element; and a device coupling part arranged on the bio-amine detection On the element, and the element joint is combined with a package of food to be tested or a food to be tested; wherein once the bio-amine detection element contacts a food-material bio-amine vaporized gas or bio-amine water vapor, the bio-amine sensitive material is immediately used The production shows a biogenic amine index.

The bio-amine gas adsorption substrate in a preferred embodiment of the present invention is selected from a water-absorbing substrate or a biocompatible substrate.

The bio-amine gas adsorption substrate of the preferred embodiment of the present invention is made of a pulp material, a cotton material, a sponge material, a textile or any combination thereof.

The bio-amine sensitive material of the preferred embodiment of the present invention includes a shaping layer and a color changing layer.

In a preferred embodiment of the present invention, the bio-amine sensitive material is selected from mononitrated benzene ring molecular materials.

In a preferred embodiment of the present invention, the nitrolated benzene ring molecular material has one nitrated functional group, two nitroated functional groups or three nitroated functional groups.

In order to achieve the above objective, another preferred embodiment of the present invention provides a method for detecting biogenic amines in food materials, including: providing a bioamine detection element, and the bioamine detection element includes a bioamine gas adsorption substrate, and the bioamine gas adsorption base The material is used to adsorb a bio-amine vaporization gas or a bio-amine water vapor; a bio-amine sensitive material is arranged on the bio-amine gas adsorption substrate; the bio-amine detection element is combined with a package of the food to be tested or a package of the food to be tested Food; and once the bio-amine detection element contacts a bio-amine vaporized gas or bio-amine water vapor of a food material, the bio-amine sensitive material is immediately used to generate a bio-amine index.

The bio-amine index in a preferred embodiment of the present invention is a bio-amine concentration, such as 50 ppm, 100 ppm, 500 ppm, 1000 ppm or other concentrations.

The biogenic amine concentration in the preferred embodiment of the present invention includes a biogenic amine safety concentration, a biogenic amine warning concentration or a biogenic amine hazardous concentration.

In a preferred embodiment of the present invention, the safe concentration of the bio-amine is lower than 20 ppm, the warning concentration of the bio-amine is 20-50 ppm, and the hazardous concentration of the bio-amine is higher than 50 ppm.

The bio-amine index in the preferred embodiment of the present invention is a visible light color indicator, for example, the original color is green, and the color is dark red or orange-brown when it exceeds 50 ppm.

The biological amine detection element contact of the preferred embodiment of the present invention When bio-amine vaporizing gas or bio-amine water vapor in food materials, it produces a bio-amine index within at least 1 hour, or preferably produces the bio-amine index within 30 minutes, or more preferably produces the bio-amine index within 10 minutes The biogenic amine index, or more preferably, the biogenic amine index is produced at least within 5 minutes, or more preferably, the biogenic amine index is produced at least within 1 minute.

The bio-amine detection element of the preferred embodiment of the present invention includes an element coupling portion, and the element coupling portion is coupled to a package of a food to be tested or a food to be tested.

The element coupling part of the preferred embodiment of the present invention is an adhesive part, a label patch, a coupling buckle, a label buckle or a coupling part of other structures.

1. ‧ amine gas adsorption substrate

10‧‧‧Detection of food bio-amine gas device

11‧‧‧Component joint

11a‧‧‧Combination buckle

2‧‧‧Bio-amine sensitive materials

3‧‧‧Adhesive layer

S1‧‧‧Step

S2‧‧‧Step

S3‧‧‧Step

S4‧‧‧Step

Figure 1: A schematic side view of the device for detecting biogenic amines in foods according to the first preferred embodiment of the present invention.

Figure 2: A schematic flow diagram of a method for detecting biogenic amines in foods according to a preferred embodiment of the present invention.

Figure 3: A schematic side view of the device for detecting biogenic amines in foods according to the second preferred embodiment of the present invention.

Figure 4: A schematic side view of the device for detecting biogenic amines in foods according to the third preferred embodiment of the present invention.

In order to fully understand the present invention, preferred embodiments are exemplified below and described in detail with the accompanying drawings, and they are not intended to limit the present invention.

The method and device for detecting biogenic amines of foodstuffs of the preferred embodiment of the present invention can be appropriately selected for automated, semi-automated or non-automated processing or implemented on the surface of various agricultural, forestry, fishery, animal husbandry products, their processed products or their by-products ; Furthermore, the device for detecting biogenic amines for foodstuffs of the preferred embodiment of the present invention can be suitably applied to various related agriculture, forestry, fishery, Animal husbandry products, its processed products, its by-products or related industries, but they are not intended to limit the scope of application of the present invention.

For example, the method and device for detecting biogenic amines in foodstuffs of the preferred embodiment of the present invention are suitable for various vegetable and fruit products (such as sweet potato leaves, water spinach, green pepper, watermelon, strawberry, mango, pineapple, grape, papaya, sweet potato, radish). , Carrots, eggplants, red peppers or other fruit and vegetables products), agricultural products (for example: agricultural product peel surface, bean skin, nut shell, cheese block surface or other agricultural products) or life-time fresh products (for example: meat, seafood or other products) Fresh products], but they are not intended to limit the scope of application of the present invention.

The preferred embodiment of the present invention adopts the definition of the technical terms of "bio-amine" or "food-material bio-amine", including "histamine", "putrescine" or "cadaverine" and other amines that are emitted when the food is corrupted. In addition, "food material" is used. The technical term "bio amine gas" or "food bio amine vaporization gas" is defined as "food bio amine vapor", "food bio amine vapor" or "food bio amine vapor", but it is not used to limit the present invention range.

Figure 1 shows a schematic side view of the device for detecting biogenic amines in foods according to the first preferred embodiment of the present invention. Please refer to Fig. 1, the device 10 for detecting biogenic amine gas for food materials according to the first preferred embodiment of the present invention includes a biogenic amine gas adsorption substrate 1, a biogenic amine sensitive material 2 and a component bonding part 11, and The bio-amine gas adsorption substrate 1, the bio-amine sensitive material 2 and the element coupling part 11 are properly assembled to form the food-material bio-amine gas detection device 10.

Please refer to Figure 1 again. For example, the bio-amine gas adsorption substrate 1 includes a first surface (or upper surface, not marked) and a second surface (or lower surface, not marked). The bio-amine gas adsorption substrate 1 has a predetermined adsorption zone (for example: open gas contact zone), and the predetermined adsorption zone of the bio-amine gas adsorption substrate 1 is suitable for adsorbing a bio-amine vaporized gas (ie food material bio-amine) Vaporized gas] or a bio-amine water vapor [Instant biogenic amine steam].

Please refer to FIG. 1 again. For example, the bio-amine gas adsorption substrate 1 can be selected from a water-absorbing substrate or a biocompatible substrate. In addition, the bio-amine gas adsorption substrate 1 can be made of a pulp material, a cotton material, a sponge material, a textile, or any combination thereof (or other suitable material with water absorption function or water vapor absorption function), In order to assist the adsorption of the bio-amine vaporized gas.

Please refer to Figure 1 again. For example, the bio-amine sensitive material 2 is disposed on any suitable position on the first surface of the bio-amine gas adsorbing substrate 1 (for example: disposed in a suitable manner) to form a Biological amine detection element or element with biological amine detection function. The bioamine sensitive material 2 can optionally form a bioamine sensitive layer, and the bioamine sensitive layer includes a first surface (or upper surface, not marked) and a second surface (or lower surface, not marked).

Please refer to Figure 1 again. For example, the bio-amine sensitive material 2 includes a shaping layer and a color-changing layer (e.g., color-changing area, color-changing pattern, color-changing text or other color-changing designs), and the color-changing layer includes A bio-amine color-changing material. In addition, the biological amine sensitive material 2 can be selected from mononitroated benzene ring molecular materials, and the nitroated benzene ring molecular material has one nitrolated functional group, two nitroated functional groups, and three nitrolated functional groups. Functional groups or other materials with similar functional groups.

Please refer to Figure 1 again. For example, the element coupling portion 11 is disposed on the biogenic amine detection element, and the element coupling portion 11 is coupled to a package of a food to be tested or a food to be tested (for example, a suitable cover Set or set on the food to be tested]. In addition, the device bonding portion 11 can be optionally disposed on any suitable position of the first surface or the second surface of the bio-amine gas adsorption substrate 1.

Figure 2 shows a schematic flow diagram of the method for detecting biogenic amines in foodstuffs according to a preferred embodiment of the present invention, which includes four main steps S1 to S4, but It is not used to limit the sequence of steps of the present invention, and without departing from the scope of the present invention, the sequence of steps in the preferred embodiments of the present invention can be appropriately changed, divided, added, combined or reduced.

Please refer to Figures 1 and 2. The method for detecting biogenic amines in foodstuffs in a preferred embodiment of the present invention includes step S1: First, for example, one or more biogenic amine detection elements are provided in an appropriate manner, and the biogenic amine detection The device includes the bio-amine gas adsorption substrate 1, and the bio-amine gas adsorption substrate 1 is used for adsorbing a bio-amine vaporized gas.

Please refer to Figures 1 and 2 again. The method for detecting biogenic amines in foodstuffs in a preferred embodiment of the present invention includes step S2: Then, for example, the biogenic amine sensitive material 2 can be pre-configured in the biological The amine gas is adsorbed on the substrate 1 in order to detect the freshness of the food to be tested, its related reference index or other detection reference index.

Please refer to Figures 1 and 2, the method for detecting biogenic amines in foods according to a preferred embodiment of the present invention includes step S3: Then, for example, in food processing operations, food packaging operations, or other food operations, the biological The amine detection element can be optionally combined with any package of the food to be tested (for example, the inner surface of the package, not shown) or any food to be tested (for example, attached to any part of the food, not shown).

Please refer to Figures 1 and 2 again. The method for detecting biogenic amines in foods according to a preferred embodiment of the present invention includes step S4: Then, for example, once the bio-amine detection element contacts a bio-amine vaporized gas or a bio-amine from foods In the case of water vapor, the bio-amine sensitive material 2 is immediately used to generate a bio-amine index, and the bio-amine index is a change that can be recognized by the naked eye. The method for detecting biogenic amines of food materials in a preferred embodiment of the present invention does not need to touch the food materials of biogenic amines or their exuded liquid, and it only needs to absorb the biogenic amine vaporized gas emitted by the food to be tested to detect the biogenic amines of food materials.

Please refer to Figures 1 and 2. For example, the bioamine index can be selected as a change that can be clearly recognized by the naked eye, such as: color change, Transparency changes, display warning symbols, display warning text or other recognizable physical or chemical changes.

Please refer to Figures 1 and 2. For example, the bio-amine index can be set to a concentration of bio-amine, such as 50 ppm, 100 ppm, 500 ppm, 1000 ppm or other concentrations. For example, the US Food and Drug Administration [FDA] stipulates that the histamine content of aquatic products shall not exceed 50 ppm, and once the histamine content reaches 500 ppm, it may endanger human health.

Please refer to Figures 1 and 2, for example, the biogenic amine concentration includes a biogenic amine safety concentration, a biogenic amine warning concentration, or a biogenic amine hazardous concentration. The safety concentration of the biological amine is lower than 20 ppm, the warning concentration of the biological amine is 20-50 ppm, and the hazardous concentration of the biological amine is higher than 50 ppm. The biogenic amine index is a visible light color indicator, for example: the original color is green, and more than 50 ppm is dark red or orange-brown, but it is not intended to limit the scope of the present invention.

For example, if the biogenic amine concentration of the food to be tested is close to 50 ppm (i.e. the biogenic amine warning concentration), you can choose to use the food to be tested as the next shelf food and further process the off shelf food into another process Food materials or processed foods to reduce the excessive bio-amine concentration of the food materials to be tested and cause improper waste of food materials.

When the bio-amine detection element of the preferred embodiment of the present invention contacts the bio-amine vaporized gas or bio-amine water vapor of food materials, it will produce a bio-amine index within at least 1 hour, or preferably at least within 30 minutes. Index, or more preferably at least within 10 minutes to produce the biogenic amine index, or more preferably at least within 5 minutes, to produce the biogenic amine index, or more preferably at least within 1 minute.

Fig. 3 shows a schematic side view of the device for detecting biogenic amines in foods according to the second preferred embodiment of the present invention. Please refer to Figure 3, compared to the first embodiment, the second preferred embodiment of the present invention is a device for detecting biogenic amines in food Optionally, at least one adhesive layer 3 is disposed on the second surface (or lower surface) or other positions (first surface or upper surface) of the bio-amine gas adsorption substrate 1.

Please refer to Figure 3 again. For example, compared to the second embodiment, another preferred embodiment of the present invention can optionally provide the adhesive layer 3 on the first surface of the bioamine sensitive material 2 [or The upper surface], and the bio-amine gas adsorption substrate 1 is used to adsorb bio-amine gas, bio-amine vaporized gas or bio-amine water vapor.

Figure 4 shows a schematic side view of the device for detecting biogenic amines in foods according to the third preferred embodiment of the present invention. Please refer to FIG. 4, compared to the first embodiment, the bio-amine detecting device for food materials in the third preferred embodiment of the present invention selects a coupling buckle 11a on one side of the bio-amine gas adsorption substrate 1.

Please refer to Figures 1 and 4. For example, in another preferred embodiment of the present invention, the component bonding portion 11 can be selected as an adhesive portion and a label patch (suitable for a variety of non-aqueous Ingredients), a label buckle (applicable to all kinds of aquatic products or all kinds of meat) or other structural joints.

The foregoing preferred embodiments only illustrate the present invention and its technical features. The technology of this embodiment can still be implemented with various substantially equivalent modifications and/or alternatives; therefore, the scope of rights of the present invention is subject to patent application. The scope defined by the scope shall prevail. The copyright in this case is restricted to the use of patent applications in the Republic of China.

1.‧‧Bio-amine gas adsorption substrate

10‧‧‧Detection of food bio-amine gas device

11‧‧‧Component joint

2‧‧‧Bio-amine sensitive materials

Claims (10)

A device for detecting biogenic amines for food materials, comprising: a bio-amine gas adsorption substrate with a predetermined adsorption zone, and the predetermined adsorption zone of the bio-amine gas adsorption substrate is used to openly adsorb a bio-amine vaporized gas or a bio-amine Water vapor; a bio-amine sensitive material which is arranged on the predetermined adsorption area of the bio-amine gas adsorption substrate to form a bio-amine detection element; and an element coupling part, which is arranged on the bio-amine detection element, and The component coupling part is combined with a package of a food material to be tested or a food material to be tested; wherein once the predetermined adsorption area of the bio-amine gas adsorption substrate of the bio-amine detection element is opened to contact a food material bio-amine vaporized gas or bio-amine water vapor The bio-amine vaporization gas or bio-amine water vapor of the food material can contact the bio-amine sensitive material via the bio-amine gas adsorption substrate, and immediately use the bio-amine sensitive material to generate a bio-amine index. According to the first item of the scope of patent application, the food bio-amine detection device, wherein the bio-amine gas adsorption substrate is selected from a water-absorbing substrate or a biocompatible substrate. According to the first item of the scope of patent application, the food bio-amine detection device, wherein the bio-amine gas adsorption substrate is made of a pulp material, a cotton material, a sponge material, a textile or any combination thereof. According to the first item of the scope of patent application, the bio-amine detection device for food materials, wherein the bio-amine sensitive material includes a shaping layer and a color changing layer. According to the first item of the scope of patent application, the biological amine detection device for food materials, wherein the biological amine sensitive material is selected from mononitrobenzene ring molecular materials. A method for detecting biogenic amines in foods, comprising: providing a biogenic amine detecting element, and the biogenic amine detecting element includes a biogenic amine gas adsorption substrate, and a predetermined adsorption area of the biogenic amine gas adsorption substrate is used to open and adsorb a Bio-amine vaporization gas or a bio-amine water vapor; disposing a bio-amine sensitive material on the predetermined adsorption area of the bio-amine gas adsorption substrate; The bio-amine detection element is bonded to a package of food to be tested or a food to be tested with an element coupling part; and once the predetermined adsorption area of the bio-amine gas adsorption substrate of the bio-amine detection element is opened to contact a food material, the bio-amine is vaporized In the case of gas or bio-amine water vapor, the bio-amine vaporized gas or bio-amine water vapor of the food material can contact the bio-amine sensitive material through the bio-amine gas adsorption substrate, and the bio-amine sensitive material is immediately used to generate a bio-amine index. According to the method for detecting biogenic amines in food materials according to item 6 of the scope of patent application, the biogenic amine index is a biogenic amine concentration. According to the method for detecting biogenic amines in food materials according to item 7 of the scope of patent application, the biogenic amine concentration includes a biogenic amine safety concentration, a biogenic amine warning concentration or a biogenic amine hazardous concentration. According to the method for detecting biogenic amines of food materials according to the sixth item of the scope of patent application, the biogenic amine detecting element includes an element coupling part, and the element coupling part is coupled to a packaging of a food material to be tested or a food material to be tested. According to the method for detecting biogenic amines of food materials according to the 9th item of the scope of patent application, the component coupling part is an adhesive part, a label patch, a coupling buckle, a label buckle or a coupling part of other structures.

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