TWI659694B - A pesticidal composition - Google Patents

Abstract

The present invention provides an insecticidal composition comprising spirobudiclofen and diafenthiuron. The composition has a synergistic effect for controlling pests. A method of controlling pests at a location is also provided, the method comprising administering to the location a combination of spiramyl diester and filfenone.

Description

Insecticide composition

The invention relates to an insecticidal composition and a method for using the same, and also relates to a fast and effective insecticidal method.

Spirobudiclofen, common chemical name: 3- (2,4-dichlorophenyl) -2-oxo-1-oxospiro [4,5] -dec-3-ene-4- Dibutyl carbonate, its molecular structure is: Snail mite diester is a new special window acid insecticide, which has a wide insecticidal spectrum and a long holding period. It kills larvae by interfering with lipid biosynthesis in insects. There is no cross-resistance between spirotepidate and existing acaricides. Due to its unique mechanism of action, spirotecan diesters can effectively control harmful mites that are resistant to existing acaricides. Snail mite diesters are highly adaptable and can be used to control a range of agricultural crops, including citrus, grapefruit, apple, pear and other fruit trees, and eggplant, pepper, tomato and other solanaceous crops. yellow spider mites, yellow tea mite (Polyphagotarsonemus latus), carmine spider mite (Tetranychus cinnabarinus), psyllids (psyllids), whitefly (Trialeurodes vaporariorum) and so on; especially strong reproductive capacity pear psylla, spider mites have a good Killing effect.

Diafenthiuron, also known as acaricidal, has a common chemical name: 1-tert-butyl-3- (2,6-diisopropyl-4-phenoxyphenyl) thiourea. The molecular structural formula of Tiffenlon is: Tifenthron is a thiourea insecticide with acaricide activity. Teflon has systemic, contact and / or gastric toxicity and fumigation effects. Tifalone interferes with the energy metabolism of the target nervous system, destroys the basic function of the target nervous system, and inhibits chitin synthesis to achieve insecticidal and acaricidal effects. Tiffenlon has a high control effect on mites (tetranychus, orange rust mite), aphids, whitefly, leafhopper, various moth pests, etc., especially against drug-resistant Psylla chinensis , cabbage green worm ( Pierisrapae Linne) and leaf curl moth (Adoxophyes honmai) can be used in a series of crops such as trees (citrus, apple), cotton, vegetables, tea and ornamental plants.

There is a need to provide an improved pesticidal composition. In particular, it would be advantageous to provide an insecticidal composition that can reduce the application rate of the active ingredient and reduce or avoid adverse environmental or toxicological effects in the field while still effectively controlling pests in the target crop. In addition, the insecticidal composition should preferably have a rapid effect and / or a long-acting effect on pests.

In addition, worldwide experience with single active standard formulation insecticides has shown a high risk of developing subpopulations of resistant pests.

Therefore, the preferred insecticidal composition has a reduced application rate, a broad spectrum of activity, persistence, and delays the drug resistance of the target species.

After a large number of biological tests and screenings, the present invention unexpectedly found that the combination of spironite diester and fenfenlong can effectively prevent and / or control pests. The activity of the combination to prevent and / or control pests is much higher than the sum of the activities of the individual active ingredients. This unexpected effect is synergistic rather than additive.

Accordingly, one aspect of the present invention provides an insecticidal composition including a spirobirox diester and filfenone.

The present invention has surprisingly found that the combination of spirobirox diester and filfenone not only broadens the insecticidal spectrum, but also shows a synergistic effect.

The term "synergistic effect" as used herein means that the insecticidal effect achieved by the combination of spirotepidate and filfenone is greater than the sum of the effects of each active ingredient when applied separately from each other.

Therefore, another aspect of the present invention provides a combination of spirotepidate and filfenone with significant synergy, thereby reducing the required dose of the two active ingredients.

The insecticidal composition of the present invention has good plant compatibility and favorable constant temperature animal toxicity, and is suitable for preventing and / or controlling harmful organisms encountered in the fields of agriculture, forestry, storage products and materials, and sanitation, especially Are insects and arachnids. The pesticidal composition of the present invention is active against pest species (including all developmental stages) that are sensitive and / or resistant to existing treatments, and can be used to control these species.

As used herein, the term "pest" means a plant can cause damage to any organism, including the bio-: Lepidoptera (Lepidoptera), for example, rice stem borer (Chilo suppressalis), yellow rice borer (Tryporyza incertulas), rice longitudinal Cnaphalocrocis medinalis Guenee, Hellulla undalis, Conogethes punctiferlis, Papilio xuthus, Pieris rapae crucivora, Parnara guttata ), Malacosoma Neustria testacea, Lymantria dispar, Hyphantria cunea, Euproctis subflava, Scopelodescontracus, Helicoverpa armigera, Red bell wheat Moth (Pink bollworm), Trichoplusia ni, Cabbage night moth (Mamestra brassicae), Spodoptera exigua, Spodoptera litura, Adoxophyes orana fasciata, Leaf Moth (Adoxophyes honmai), Apricot Yellow Moth (Archipsfuscocureanus), Tea Long Roll Moth (Homona magnanima), Diamondback Moth (Plutella xylotella), Cotton Red Bell (Pectinophora gossypiella); Coleoptera (Coleoptera), for example, rice weevil (Sitophilus oryzae linne), citrus leaf miner A (Podagricomela nigricollis Che), maize weevil (S.zeamails), Grain Weevil (S.granarius), Colaphellus Cabbage leaf beetle, Daikon leaf beetle, Flea beetle, Altica chalybea, Phyllotreta striolata, Epitrix cucumeris, Tobacco flea beetles (Ehirtipennis), eggplant flea beetle (E.fuscula), yellow melon (Aulacophora indica (Gemlin), mustard leaf beetle (Phaedon cochleariae) and so on; Diptera (Diptera), for example, rice leafminer (Agromyza oryzae) , Hydrellia griseola, Liriomyza trifolii, Chromatomyia horticola, Liriomyza bryoniae, Delia platura, Onion ground fly (Delia antiqua) Ceratitis capiata wiedgman, Rhagoletis pomonella, R.cingulata; Hemiptera pests, such as Megacopta punctatissimum, Eurydema rugosum ,Big Eysarcoris lewisi, Eysarcoris parvus, Nezara viridula, Plautia stali, Halymorphamista, Cletus punctiger, Chinese rice margin bug (Leptocorisachinensis), azalea lace bug (Stephantis pyrioides), Akasu bugs (Trigonotylus coelestialium); Homoptera (Homoptera), e.g., green rice leafhopper (Nephotettix cincticeps), small brown planthopper (Laodelphax striatellus), brown rice fly Nilaparvata lugens, Sogatella furcifera, Psylla pyrisuga, Diaphorinatabaci, Bemisia tabaci, Homoptera, Eriosoma lanigerum; Hymenoptera includes, for example, Athalia rosae ruficornis, Arge pagana, Formica japonica, Dryocsmus kuriphilus, etc .; Physanoptera), such as Haplothrips Chinensis Priesner; Arachnida, such as Panonychus citri, Tetranychus cinnabarinus, apple Claw mite (Panonychus ulmi), Tetranychus urticae, Tetranychus viennensis, Oligonychus ununguis, Eotetranychus kankitus, Brevipalpus phoenicis, Bryobiapraetiosa, Aceria tulipae, Colomerus vitis, Calacarus carinatus, Polyphagotarsonemus latus, Rhizoglyphus rostochiensis Etc .; Anoplura (Phthiraptera), for example, Damalinia spp., Haematopinus spp., Linognathus spp., Pediculus spp., Chewing lice (Trichodectes spp.); Isopoda, for example, Oniscus asellus, Armadilliudium vulgare, Porcellio scaber; Diplopoda, for example, millipede Blaniulus guttulatus); Chilopoda, for example, Geophilus carpophagus, Scutigera spp .; Symphyla, for example, Scutigerella immaculata; Thysa nura), for example, Lepisma saccharina; Collembola, for example, Onychiurus armatus; Orthoptera, for example, Acheta domesticus, Polygonum Gryllotalpa spp., Locusta migratoria migratorioides, Melanopluss spp., Schistocerca gregaria; Blattaria, for example, Blatt orientalis Periplaneta americana, Leucophaea maderae, Blattella germanica; Dermaptera, for example, Forficula auricularia; Isoptera, for example, Reticulitermes spp .; Nematoda, for example, Meloidogyne, Heterodera schachtii, Rhabditis strongyloides, and Aphelenchoides fragariae The following nematodes: soybean cyst nematodes (Heterodera), potato gold nematodes (Golbodera rostochiensis), Meloidogyne incognita, etc .; Dorylaimida , For example, Longidorus sp.

The insecticidal composition of the present invention has outstanding effects on prevention and / or control of Helicoverpa armigera, aphids, red spiders, psyllids, moths, and leaf miners.

By combining two active ingredients with different modes of action, the combination has the effect of killing target pests through a series of effects, such as through contact effects, gastric toxicity, egg-killing effects, and reduction of spawning rate and hatchability.

The active ingredients spirobirox diester and filfenone can be present in a wide range of weight ratios or used together. More specifically, the weight ratio of the spiramite diester and filfenone is about 100: 1 to about 1: 100, preferably about 50: 1 to about 1:50, and more preferably about 25: 1 to about 1 : 25. A more preferred ratio is about 20: 1 to about 1:20, more preferably about 15: 1 to about 1:15, more preferably about 10: 1 to about 1:10, and even more preferably about 5: 1 to About 1: 5.

The insecticidal composition of the present invention may include a wide range of spirobirox diesters and filfenone. The amount of active ingredient depends, for example, on the type of formulation used. In various embodiments, the composition comprises spirobirox diester and filfenone in an amount of at least 5% by weight, preferably at least 10% by weight, more preferably at least 15% by weight, more preferably at least 20% by weight %, More preferably at least 25% by weight. It can also be formulated to contain at least 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85% or 90% Composition by weight. The composition comprises spirobirox diester and filfenone in an amount of about 5% to about 90% by weight of the composition, preferably about 10% to about 80% by weight of the composition, and more preferably about 15%. % To about 60%, more preferably about 20% to about 60% by weight of the composition.

In addition to the above-mentioned active compounds spirobirox diester and fenfenlon, the pesticidal composition may contain one or more other active ingredients.

The pesticidal composition can be prepared in a conventional manner, for example, by mixing the active ingredient with a suitable adjuvant. Suitable adjuvants that can be included in the composition of the invention are all conventional formulation adjuvants or components, such as fillers, vehicles, solvents, surfactants, stabilizers, defoamers, antifreeze agents, preservatives, anti- Oxidants, colorants, thickeners, solid adhesives, anti-aggregation agents and inert fillers. Such auxiliaries are known in the art and are commercially available. The formulations can be prepared in a known manner by mixing the active ingredients with suitable fillers and / or surfactants suitable for use in agrochemical formulations.

The insecticidal composition of the present invention can be converted into conventional formulations, such as suspending agents (SC), seed coating agents, wettable powders (WP), water-dispersible granules (WG), microcapsule suspension agents (CS), Coated granules, extruded granules, emulsifiable concentrates (EC), microemulsions (ME), emulsions, water emulsions (EW), effervescent tablets (PP), smoke candles (FK), ultra-low volume liquids (LDL) The use form depends on the particular purpose; each formulation should ensure uniform distribution of the compounds of the composition of the invention. Techniques for formulating compositions in these ways are known in the art.

According to the present invention, the term "bulking agent" refers to a natural or synthetic organic or inorganic compound that can be combined or combined with an active ingredient to make it easier to apply to a subject (e.g., plant, crop, or grass, etc.) Thing. Therefore, the filler is preferably inert, at least agriculturally acceptable. The filler can be solid or liquid. Suitable solid fillers include plant-based powders (e.g. soybean flour, starch, cereal flour, wood flour, bark flour, sawdust, walnut shell flour, bran, cellulose powder, coconut shell, corn cobs, and pellets of tobacco stalks) , Residues after extracting plant extracts, etc.), paper, clay (such as kaolin, bentonite, acid porcelain, etc.), talc, silica (such as diatomite, silica sand, mica, hydrous silicic acid, calcium silicate ), Activated carbon, natural minerals (pumice, attapulgite, zeolite, etc.), fired diatomaceous earth, sand, plastic media (such as polyethylene, polypropylene), inorganic mineral powders (such as potassium chloride, Calcium carbonate, calcium phosphate, etc.), chemical fertilizers (such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, etc.), and soil fertilizer. These can be used alone or in combination of two or more.

Suitable liquid carriers are selected from water, alcohols (e.g. methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (e.g. acetone, methyl ethyl ketone, diisobutyl ketone, cyclohexanone) Etc.), ethers (such as diethyl ether, dioxane, methyl cellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (such as kerosene, mineral oil, etc.), aromatic hydrocarbons (such as benzene, toluene, xylene, and solvent oil) , Alkyl naphthalene, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amidines, amidines, mineral oils, vegetable oils, animal oils, etc.

To emulsify, disperse, and / or wet the active ingredient, a surfactant may be used in the formulation. The choice of one or more surfactants depends, for example, on the type of formulation used and the desired end-use mode of the composition. Suitable surfactants are known in the art and include compounds such as fatty alcohol polyoxyethylene ethers, polyoxyethylene alkylaryl ethers, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phosphate esters of phenols, polybasic Fatty acid esters of alcohols, alkyl aryl sulfonates, lignin sulfonates, polymer comb-shaped branched copolymers, butyl naphthalene sulfonates, alkyl aryl sulfonates, alkyl sulfosuccinates Acid salt.

To stabilize the dispersion of the active ingredient, a stabilizer may be included in the formulation. Suitable stabilizers are known in the art and include xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose ether, polyvinyl alcohol, polyvinyl acetate and natural phospholipids (such as brain phospholipids and lecithin), and synthetics Phospholipids, bentonite and lignosulfonates.

Suitable antifreeze agents are known in the art and include ethylene glycol, propylene glycol, glycerol and sorbitol.

Anti-aggregation agents are known in the art and include naphthalenesulfonic acid polymers, polymeric phosphates, and the like.

Defoaming agents are also known in the art. A suitable defoamer is a silicone defoamer.

Optionally, other additional components may be included in the composition, such as protective colloids, adhesives, thickeners, thixotropic agents, penetrants, stabilizers, masking agents, as needed.

The active ingredients of spirulina diester and filfenone of the present invention can also be applied in combination with other active ingredients, thereby expanding the spectrum of activity or preventing the formation of drug resistance. The other active ingredients are, for example, fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or chemical pheromones.

In another aspect, the present invention provides a method of preventing and / or controlling a pest in a location, the method comprising applying a combination of spirobirox and diefenol to the location.

In the method, the spirobirox diester and filfenone can be applied to the site simultaneously or at different times (ie, separately, sequentially, or simultaneously). When applied separately, the order of application generally does not affect the control effect.

Spiromite diesters and filfenone can be applied to the sites using the aforementioned weight ratios.

In one embodiment, the method comprises applying to the site a composition comprising a spiral mite diester and filfenone, such as the aforementioned insecticidal composition.

The location may be the target plant or its surroundings, such as the surrounding soil or space, the target pest or its environment, or the propagation material of the target plant.

The term "plant propagation material" as used herein refers to all growing parts of a plant, including seeds, roots, fruits, tubers, bulbs, rhizomes, shoots, shoots and other plant parts. Seedlings and young plants transplanted after germination or after emergence from the soil may also be included. Plant propagation material can be treated with plant protection compounds before planting or at or before transplantation.

A method for protecting crops from harmful organisms, said method comprising contacting the crop and / or its surroundings with an insecticidal composition comprising a spirulina diester and filfenone.

The insecticidal composition of the present invention is particularly important in preventing and / or controlling most harmful organisms on various cultivated plants such as cereals, fruits, vegetables, and cash crops.

Plants which can be treated according to the invention include all genetically engineered plants or transgenic plants.

As mentioned above, the combination of spirobirox and diefenuron, for example by means of insecticidal compositions, can be used to protect plant propagation material and subsequently grown plant organs.

In another aspect, the present invention provides the use of spirodeipyl diesters and filfenone to control pests at a certain location.

The pesticidal composition and method of the present invention are used to protect all parts of a target plant from pests, for example, by treating leaves and shoots, treating seeds and roots to prevent soil pests. Therefore, the insecticidal composition and method of the present invention are suitable for seed treatment to protect seeds from insects and pests, especially soil insects and pests, and to protect the roots and shoots of plants from soil pests and foliar insects. .

Preferably, the plant propagation material is a seed. The method of seed treatment includes soaking the seed with a composition of an active ingredient, such as a dilute liquid preparation or a dilute solid preparation or soaking the seed with an undiluted liquid preparation; mixing the solid or liquid preparation with the seed to coat the preparation with the surface of the seed; and Spray the formulation of the active ingredient at the location where the seeds are planted.

During the practice of the methods and uses of the present invention, the active ingredients can be applied by techniques known in the art, including canning, spraying, spraying, dusting, spreading or smoking. The amount of active ingredient applied can vary depending on factors such as weather conditions or the condition of the crop to be treated.

Generally for leaf treatment, the dosage rate of the active ingredient is usually 0.1-10000 grams of active ingredient per hectare (g A.I./ha), preferably 10-1000 g A.I./ha, more preferably 50-800 g / ha. For seed treatment, the dosage is usually 2-1000 g A.I./100 kg seeds, preferably 3-300 g A.I./100 kg seeds. For soil treatment, the dosage is usually 0.1-10000 g A.I./ha, preferably 1-5000 g A.I./ha.

The above-mentioned dosages are merely general exemplary dosages. Those skilled in the art will adjust the application rate according to the actual situation and needs, especially according to the nature of the plant or crop to be treated and the severity of the pest.

Pest control is preferably carried out in the early stages of pest occurrence. However, in many cases it is not possible to detect pests before they have caused severe damage to the crop or plant and can be observed. By using the combination of the spirobirox diester of the present invention and filfenone, the composition has a rapid effect, and the maximum pest mortality is reached within 12 hours after administration. Once applied, the effect of the combination of active ingredients can be maintained for up to 45 days.

On the other hand, the use of the combination of active ingredients of the present invention can delay the development of target plant resistance.

The embodiments of the present invention are described below in a descriptive manner only.

In the examples, all percentages are based on the weight of the composition, unless stated otherwise.

Examples Formulation examples Example 1: 5% spiromidate diester + 10% filfenone (oil suspension)

An oil suspension was prepared having the composition shown in the following table.

The components are mixed uniformly, and an oil suspension agent of 5% spiromidate and 10% filfenone is obtained after grinding and / or high-speed shearing.

Example 2 30% spirotepidate diester + 60% filfenone (wettable powder)

A wettable powder having the composition shown in the following table was prepared.

The components are mixed and pulverized by an ultrafine pulverizer to obtain a wettable powder of 30% spironite diester + 60% filfenone.

Example 3 20% Spirocarboximide + 60% Teflon (wettable powder)

A wettable powder having the composition shown in the following table was prepared.

The components are mixed and milled by an ultra-fine pulverizer to obtain 20% spirotepidate + 60% filfenone wettable powder (WP).

Example 4 10% spirotepidate + 50% fenfenlon (water-dispersible granules)

Water-dispersible granules having the composition shown in the following table were prepared.

The components are mixed and pulverized into a wettable powder by spraying. Water is added and mixed with the wettable powder to form a paste, which is then extruded and dried to obtain 10% spirotepidate + 50% filfenone water-dispersible granules (WG).

Example 5 2% Spiroxyl Diester + 3% Teflon (emulsion)

An emulsifiable concentrate preparation having the composition shown in the following table was prepared.

The components were stirred to obtain a homogeneous phase.

Example 6 2% Spirospiram diester + 20% Teflon (seed coating agent)

A seed coating agent having a composition shown in the following table was prepared.

The components are mixed uniformly, and then ground and / or high-speed shearing is performed to obtain 2% spiromidate diester + 20% fenfenlong seed coating agent.

Example 7 10% Spirocarboximide + 10% Teflon (wettable powder)

A wettable powder having the composition shown in the following table was prepared.

The components are mixed, ground, and pulverized to obtain 10% spiramyl diester + 10% filfenone wettable powder (WP).

Example 8 5% spiromidate diester + 35% filfenone (water-dispersible granules)

Water-dispersible granules having the composition shown in the following table were prepared.

The components are mixed uniformly, and then pulverized into a wettable powder by spraying. Thereafter, water was added and mixed, and the obtained mixture was extruded and dried to obtain 5% spiromidate diester + 35% filfenone water dispersible granules (WG).

Example 9 5% spiromidate diester + 10% filfenone (suspension emulsion)

A suspoemulsion having the composition shown in the following table was prepared.

The spiral mite diester was dissolved in methyl oleate, and then ethoxylated castor oil was added to obtain an oil phase of the spiral mite diester. Teflon, disodium methylene dinaphthalene sulfonate, modified calcium lignin sulfonate, xanthan gum, bentonite, glycerol and water are mixed uniformly, and sand-milled to prepare a Teflon suspension. The oil phase was added to the suspension phase and mixed to obtain 5% spiromidate diester + 10% filfenone suspension emulsion (SE).

Example 10 2% Spirocarid Diester + 8% Teflon Coated Granules

Coated granules having the composition shown in the following table were prepared.

In a mixer, the finely divided active ingredient is uniformly applied to a carrier wetted with polyethylene glycol to obtain a dust-free coated granule.

Example 11 20% Spirocarbox diester + 40% Teflon Extrusion Granules

Extruded granules having the composition shown in the following table were prepared.

The active ingredient is mixed with the auxiliary agent and ground, wet with water, extruded, and then dried in air to obtain granules.

Example 12 10% Spirospirin diester + 90% Teflon

A mixture formulation having the composition shown in the following table was prepared.

Mix the spiramite diester and filfenone evenly.

Biological test cases

After mixing different types of pesticide active ingredients, they usually show three types of action: additive action, synergistic action and antagonistic action. The type of effect is usually unpredictable and can only be known through extensive experimental screening. The inventors experimented with different formulations of spiramyl diester and filfenone. The insecticidal composition was found to have a synergistic effect, rather than an additive or antagonistic effect.

The indoor toxicity test was used to determine the toxicity index of each test preparation and the co-toxicity coefficient (CTC value) of the mixed preparation. When CTC At 80, the composition is considered to exhibit antagonism. When the CTC value obtained is 80 CTC At 120, the composition is considered to exhibit an additive effect. When CTC At 120 hours, the composition is considered to exhibit a synergistic effect.

Test 1: Determination of indoor toxicity to Psyllid

experiment method:

Pear leaves of the same size that had not been exposed to any insecticidal composition were put into a box, and the pear lice larvae were attached.

A liquid formulation is prepared containing a certain range of spiromidate diester and filfenone.

In each test, the leaves were immersed in the liquid formulation for 5 seconds. Let the leaves dry naturally and put them in the box.

Twenty larvae were reared in the box at 25 ° C.

Control experiments were performed under the same test conditions.

Each test was repeated 3 times.

After 72 hours, check the number of dead insects. Calculate the mortality rate (%), adjust the mortality rate (%) and the LC ₅₀ value, and get the following toxicity regression equation: mortality rate (%) = (number of live insects before application-number of live insects after application) / live before application Number of insects * 100 corrected mortality (%) = (treatment group mortality-control group mortality) / (100-control group mortality) * 100

The corrected mortality of the tested insects was converted into a probability value (y), and the concentration (μg / ml) was converted into a logarithmic value (x). Co-toxicity coefficient (CTC) was calculated according to the Sun Yunpei formula. The calculation formula is as follows (using Spirocarbox diester as standard drug, its toxicity index is 100): actual toxicity index (ATI) = (standard drug LC ₅₀ / test drug LC ₅₀ ) × 100 theoretical toxicity index (TTI) = A drug ATI × percentage of A in the mix + B agent ATI × percentage of B in the mix Co-toxicity coefficient (CTC) = (mix ATI / mix TTI) × 100

If the mortality rate in the control group is greater than 10%, the test is considered ineffective.

The results of the indoor toxicity test are as follows:

From the results in Table 1, it is clear that the co-toxicity coefficients of the compositions containing both spiramyl diester and filfenone are all greater than 120. The composition thus exhibits a synergistic effect.

Test 2: Laboratory Toxicity to C. citrus

experiment method:

The citrus leaves were cut off with handles and inserted into watered ampoules. The leaves were infested by a certain number of adult citrus mites. After the adult mites lay their eggs for 24 hours, the adult mites are removed.

A liquid formulation is prepared containing a certain range of spiromidate diester and filfenone.

In each test, leaves with enough eggs were immersed in the liquid formulation for 10 seconds. Remove excess liquid preparation with paper. Place the leaf back in the ampoule and let it air dry. Cultivate ampoules.

Each test was repeated 4 times.

Perform a control experiment.

After the incubation in the control experiment is complete, investigate the condition of the eggs and any larvae that appear. Juvenile mites that cannot hatch and / or crawl normally are considered dead.

The results of indoor toxicity tests are shown in Table 2:

From the results in Table 2, it is clear that the co-toxicity coefficients of the compositions of the present invention are all greater than 120, and therefore have a synergistic effect.

Field trial

When the activity of the composition exceeds the sum of the activities when each active ingredient is applied alone, there is a synergistic effect in the composition containing the two active ingredients. The expected effect of a particular combination of two active ingredients can be calculated using the so-called "Colby formula" (see SRColby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations", Weeds 1967, 15, 20-22) as follows: If X is the active Lethal rate (%) of the untreated control when ingredient A is used at an application rate of mg / ha or a concentration of m ppm, and Y is the lethality of the untreated control when the active ingredient B is used at an application rate of ng / ha or a concentration of n ppm (%), E is the lethality (%) of the untreated control when the active ingredients A and B are used at application rates m and ng / ha or concentrations m and n ppm, then

If the actual lethality observed exceeds the calculated value, the activity of the composition is super-additive, in other words, there is synergy.

Field trials for controlling Psylla:

The test time is late March. The test was performed during the incubation period of P. pylori eggs (hatching rate exceeded 60%). A formulation containing a range of spirulina diesters and filfenone was sprayed on the front and back of pear leaves. Each experiment treated 3 pear trees. The number of live insects on the leaves was investigated with binoculars 1 day, 7 days, 21 days, and 45 days after application.

Calculate the lethal rate of insects and the control effect of each composition, the formula is as follows: the lethal rate of insects (%) = (number of live insects before application-number of live insects after application) * 100 ) = (Pestlet mortality rate in the treatment group-Blank control group fatality rate) * 100 / (100- Blank control group fatality rate)

Field test results are shown in Table 3 below:

From the results of the tests in Table 3, it can be seen that the observed control effect of the composition containing spirotepidate and filfenone is significantly higher than the expected control effect. Compositions containing spirobirox diester and fenfenlon exhibited significant synergistic effects. And notice that the effect is fast and long-lasting.

Claims (19)

An insecticidal composition comprising a compound of formula (I) And a compound of formula (II), The pesticidal composition according to claim 1, wherein a weight ratio of the active ingredient of the formula (I) and the active ingredient of the formula (II) is 100: 1 to 1: 100. The pesticidal composition according to claim 2, wherein a weight ratio of the active ingredient of the formula (I) and the active ingredient of the formula (II) is 50: 1 to 1:50. The pesticidal composition according to claim 3, wherein the weight ratio of the active ingredient of the formula (I) and the active ingredient of the formula (II) is 25: 1 to 1:25. The pesticidal composition according to claim 4, wherein a weight ratio of the active ingredient of the formula (I) and the active ingredient of the formula (II) is 10: 1 to 1:10. The pesticidal composition according to claim 5, wherein a weight ratio of the active ingredient of the formula (I) and the active ingredient of the formula (II) is 5: 1 to 1: 5. The pesticidal composition according to any one of claims 1 to 6, wherein the active ingredient of the formula (I) and the active ingredient of the formula (II) together constitute 5% to 90% by weight of the composition. The pesticidal composition according to claim 7, wherein the active ingredient of the formula (I) and the active ingredient of the formula (II) together constitute 10% to 80% by weight of the composition. The pesticidal composition according to claim 8, wherein the active ingredient of the formula (I) and the active ingredient of the formula (II) together constitute 20% to 60% by weight of the composition. The pesticidal composition according to any one of claims 1 to 6, further comprising one or more surfactants and / or fillers. The insecticidal composition according to any one of claims 1 to 6, wherein the insecticidal composition is formulated as an emulsifiable concentrate (EC), a suspending agent (SC), a seed coating agent, a water-dispersible granule (WG), Wet powder (WP), suspoemulsion (SE), aerosol (FK), (oil-in-water) emulsion (EW), ultra-low-volume liquid (LDL). A method of treating a pest at a location, said method comprising applying a compound of formula (I) to said location And a compound of formula (II), A method according to claim 12, wherein said compounds of formula (I) and formula (II) are applied to a plant, a target pest or its environment, or a propagation material of a plant. The method according to claim 13, wherein the plant propagation material is a seedling, a rhizome, a nursery, a cutting, or a seed. The method according to claim 12 or 13, wherein the pest is selected from insects and mites. The method according to claim 15, wherein the pest is selected from the group consisting of red spider and psylla. The method according to any one of claims 12 to 14, wherein the active ingredient of formula (I), the active ingredient of formula (II) are administered separately, sequentially or simultaneously. The method of any one of claims 12 to 14, wherein the method comprises administering to the location a composition of any one of claims 1-11. Use of a compound of formula (I) and a composition of formula (II) for preventing and / or controlling pests