TWI577395B - Nano - silver colloidal wound dressing film and its preparation method - Google Patents

Description

Nano silver colloid wound dressing film and preparation method thereof

The invention provides a nano silver colloid wound dressing film, in particular to a nano silver colloid wound dressing film which has high tensile tension and water absorption and has a sustained release effect on antibacterial silver ions and a preparation method thereof.

The skin is the largest organ of the human body, and it has the function of blocking the invasion of harmful substances from the outside to prevent the loss of moisture, ions and regulating body temperature. Accidental disasters (such as fires and traffic accidents) often cause damage to a wide range of skin organs, causing infection, loss of water ions, or abnormal temperature regulation and life-threatening.

Usually secondary or tertiary grades of moderate to severe burns are admitted to hospital. Medical staff often apply antibiotics to the whole body via the digestive or non-digestive tract after debridement, or local administration. The wound is coated with an antibacterial agent and covered with a layer of sterile gauze, applied with an elastic bandage.

In the initial wound, a large amount of liquid (such as blood, necrotic tissue, immune cells, etc.) will infiltrate the contaminated gauze due to exudation, so it needs to be replaced frequently. However, when the gauze is covered by the reticular fibrous gauze, the gauze easily enters the wound and the tissue is intertwined. Therefore, when the gauze is changed, it is easy to pull off the patient's newborn skin tissue cells, causing great discomfort. With pain, it is more likely to cause secondary damage and infection to the wound of the patient.

Therefore, in order to meet the needs of the development of wound dressing technology, along with the need for terminal wound recovery, and to strive for the trust and potential market of the medical end, it is necessary to develop technologies for manufacturing wound dressings, which can achieve environmental protection purposes and save manufacturing costs. And can effectively form a protective wound antibacterial dressing.

In accordance with the shortcomings of the aforementioned wound dressings, it is currently necessary to provide a new wound dressing and a method of preparing the same to solve the above problems. Therefore, the main object of the present invention is to use a biocompatible polymer as a matrix, supplemented with a specific surfactant, a plasticizer, and an antibacterial nano silver to form an antibacterial colloidal wound dressing film, and the film It has the characteristics of high tensile tension and water absorption capacity, which can replace the current use of medical gauze.

Another object of the present invention is to provide an antibacterial nano silver colloid wound dressing as a film which is easy to adhere to a wound of a patient and has a high water absorption capacity, which can reduce the frequency of replacement of the patient's wound dressing, and avoids when changing the dressing. Pull and tear off the patient's newborn skin tissue cells to avoid discomfort and reduce pain.

A further object of the present invention is to form a film using a ratio of a biocompatible amphoteric polymer to a specific formulation as a carrier for the slow release of the antibacterial agent nano silver. The antibacterial nano silver colloid wound dressing replaces the antibacterial agent and the sterile gauze to accelerate the wound healing, the high water absorption capacity reduces the dressing frequency, and the high tensile elongation makes it strong without sticking to the patient. wound.

According to the above object, the present invention discloses an antibacterial nano silver colloid wound dressing The film is composed of a biocompatible polymer, a surfactant, a plasticizer, a plurality of nano silver particles and an aqueous solution, and is characterized by: a biocompatible polymer, a surfactant, a plasticizer, and a naphthalene. The composition ratio of the rice silver particles and the aqueous solution is 30-50 (W/V%): 3-6 (W/V%): 0.3-1.6 (W/V%): 0.1-2 (W/V%): 30 -60 (V/V%). The antibacterial nano silver colloid wound dressing film composed of the ratio has a high tensile tension and water absorption capacity, and an antibacterial effect is exerted on the wound of the patient by using the antibacterial nano silver to be slowly released from the film.

According to the foregoing antibacterial nano silver colloid wound dressing film, the present invention also discloses a preparation method of the antibacterial nano silver colloid wound dressing film, which comprises the steps of: providing an ethanol solution and dissolving poly(2-hydroxyethyl methacrylate) In an ethanol solution to obtain a poly(2-hydroxyethyl methacrylate) ethanol solution, a plasticizer, a surfactant, an aqueous solution containing a plurality of nano silver particles, and a poly(2-hydroxyethyl methacrylate) ethanol solution. Mixing uniformly to obtain a mixed viscous liquid, subjecting the mixed viscous liquid to a stretching step to obtain a wet film, and removing the ethanol solution remaining in the wet film to obtain a film, wherein the film has a thickness of 0.05-0.06 mm and can be attached thereto. The patient's wound replaces the existing medical dressing used.

10‧‧‧ Providing ethanol solution

12‧‧‧ Dissolve poly(2-hydroxyethyl methacrylate) (pHEMA) in ethanol solution to obtain poly(2-hydroxyethyl methacrylate) ethanol solution (pHEMA ethanol solution)

14‧‧‧Where a plasticizer, a surfactant, a plurality of nano silver particles and an aqueous solution and a poly(2-hydroxyethyl methacrylate) solution are uniformly mixed to obtain a mixed viscous liquid.

16‧‧‧Draw a mixed viscous liquid for the stretching step to obtain a wet film

18‧‧‧Removing the ethanol solution remaining in the wet film to form a film by fixed molding

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a flow chart showing the steps of making an antibacterial nano silver colloidal wound dressing film in accordance with the teachings of the present invention.

Fig. 2 is a schematic view showing the test of the tensile length of the tensile strength test of the antibacterial nano silver colloid wound dressing film according to the technique disclosed in the present invention.

Figure 3 is a graphical representation of the test for the release of nano silver ions in an antibacterial nanosilver colloidal wound dressing film in accordance with the teachings of the present invention.

The invention discloses an antibacterial nano silver colloid wound dressing film, which is composed of a biocompatible polymer, a surfactant, a plasticizer, a plurality of nano silver particles and an aqueous solution, wherein the biocompatible polymer, The composition ratio of the surfactant, the plasticizer, the plurality of nano silver particles and the aqueous solution is 30-50 (W/V%): 3-6 (W/V%): 0.3-1.6 (W/V%): 0.1 -2 (W/V%): 30-60 (V/V%).

In the present invention, the biocompatible polymer used may be a hydrogel having a highly water-containing polymer substance, soft in texture such as rubber, low interfacial tension to make it highly adhesive, and easy to adhere. On the surface of other substances. In the present invention, the main component of the biocompatible polymer is poly(2-hydroxyethyl methacrylate), which means 2-hydroxyethyl methacrylate. The polymer of the repeating unit has a chemical formula of (C ₆ H ₁₀ O ₃ ) _n , a molecular weight of 25,000 to 100,000 Da, and a dispersibility ranging from 2 to 3.8. Usually in the aqueous phase, which uses its asymmetric carbon at the center as a fulcrum, the polar hydroxyethyl group turns to the outside and thus becomes flexible; in the air, the non-polar methyl group turns to the outside, thus making it easier Brittle. Poly(2-hydroxyethyl methacrylate) can form an aqueous gel by containing water, but does not dissolve in water, and is therefore a material for making artificial crystals. Crosslinked prepolymers are utilized to make a wide variety of articles, including hydrophilic coatings, soft contact lenses, and high precision molded articles. Another device can be used as a drug carrier molecule, such as IUD (NuvaRing ^TM), the ring means disposed in the cervix, sustained release of etonogestrel and ethinyl estradiol interference pregnancy, medical polymer materials are also useful.

In addition, a surfactant (Surfactant), or surfactant, is a kind of energy The substance which causes the surface tension of the target solution to be significantly lowered can reduce the surface tension between the two liquids or the liquid and the solid, and prevent the small droplets of the dispersed phase from agglomerating and coagulating. The surfactant is generally an organic amphiphilic molecule having hydrophilic and hydrophobic groups, and is soluble in water or an organic solvent. It is generally divided into four categories: anionic, cationic, nonionic and zwitterionic, which can be used as detergents, wetting agents, emulsifiers, foaming agents and dispersing agents, and has a wide range of applications, including cosmetics and household products. , food industry, pharmaceutical and medical industry, etc. Both Span and Tween are nonionic surfactants of sorbitan esterified derivatives, used as emulsifiers, co-emulsifiers, solubilizers, dispersants in the pharmaceutical and food industries, and are stable over a wide range of pH values. good.

The surfactant used in the present invention may be Span or Tween, wherein Span is a series of sorbitan esterified fatty acid derivatives (Sorbitan esters), which are commonly classified as: Span 20 (Sorbitan monolaurate), Span 40 ( Sorbitanmonopalmitate), Span 60 (Sorbitanmonostearate), Span 80 (Sorbitanmonooleate), Span 83 (Sorbitansesoleoleate), Span 85 (Sorbitantrioleate), Span 120 (Sorbitanisostearate), wherein the number next to Span represents different fatty acids, such as monolaurate It is represented by 20 that monopalmitate is represented by 40, monostearate is represented by 60, monooleate is represented by 80, sesquioleate is represented by 83, and trioleate is represented by It is represented by 85, and isostearate is represented by 120. The Span series has a hydrophilic-lipophilic balance (HLB Value) ranging from 1.8 to 8.6 and is suitable for water-in-oil (W/O) interfaces with high hydrophobicity values.

The Tween used in the present invention is a series of polyethoxylated sorbitan esterified fatty acid derivatives (Polyoxylatedsorbentan esters), which are commonly classified as: Tween 20 (Polyoxyethylene-20 sorbitan monolaurate), Tween 21 (Polyoxyethylene- 4 sorbitanmonolaurate), Tween 40 (Polyoxyethylene-20 sorbitan monopalmitate), Tween 60 (Polyoxyethylene-20 sorbitan monostearate), Tween 61 (Polyoxyethylene-4 sorbitan monostearate), Tween 65 (Polyoxyethylene-20 sorbitantristearate), Tween 80 (Polyoxyethylene-20 sorbitan monooleate), 4 Or 20 refers to the total number of oxyethylene (-(CH ₂ CH ₂ O)-) monomers, wherein the Tween side number represents a different fatty acid, such as monolaurate is represented by 20 or 21, single palm The acid ester is represented by 40, the monostearate is represented by 60 or 61, the tristearate is represented by 65, and the monooleate is represented by 80. The Tween series has a hydrophilic-lipophilic balance (HLB Value) ranging from 9.6-16.7, which is suitable for the oil-in-water (O/W) interface and is higher in hydrophilicity than the Span series.

The plasticizer used in the present invention, or plasticizer or plasticizer, is an additive for increasing the softness of a material or liquefying a material. The added object areas include plastic materials, building materials, the food industry, the pharmaceutical industry, and so on. The same plasticizer is used on different objects and often has different effects. There are more than one hundred kinds of plasticizer seed materials. In the present invention, since the antibacterial nano silver colloid wound dressing film is attached to the wound of the human body and will contact the skin of the human body, the plastic which is safe for the human body is used. Types of agents, such as Acetylated monoglyceride, 1,2-Cyclohexane dicarboxylic acid diisononyl ester (BASF is a registered trademark of DINCH). Or Alkyl citrates, in which Acetylated monoglyceride has biodegradability and is not easily caused by biological reactions, and can be used as a food additive. In addition, 1,2-cyclohexane dicarboxylic acid diisononyl ester can be used as food packaging, medical equipment and children's toys, including plasticizers and PVC. Most of the polymers are compatible. Alkyl citrates can be used as food packaging, medical equipment, drug controlled release, cosmetics and toys, Alkyl citrates such as Triethyl citrate (TEC), citrate B Acetyl triethyl citrate (ATEC), Tributyl citrate (TBC), Acetyl tributyl citrate (ATBC), Trioctyl citrate (Trioctyl citrate, TOC), Acetyl trioctyl citrate (ATOC), Trihexyl citrate (THC), Acetyl trihexyl citrate (ATHC), Dingzhi lemon Trihexyl o- butyryl citrate (BTHC), Trimethyl citrate (TMC).

Furthermore, in the present invention, in order to impart an antibacterial function to the dressing, nano silver having an antibacterial ability is used, and it is known that nano silver has a bactericidal effect, since the activated silver of nanocrystallization can penetrate the cell membrane and The thiol group of the bacterial enzyme protein binds to block the biochemical activity of the bacterial protein to achieve a bactericidal effect, and the silver ion can also block the ability of the DNA to translate the protein and is toxic. Here, the nanometer refers to a billionth of a meter, that is, 10 ^-9 nm (nanometer), which is equivalent to the size of the nucleotide to the molecule. When the particle size of the material is continuously miniaturized, the total surface area is greatly increased, and the interface energy is large. It is unstable, so the particles tend to aggregate and form large particles. The current scientific and technological progress, the use of a protective agent to adsorb the surface of the particles can inhibit the silver particles from becoming large, as long as the particle size is less than 100 nm, it can be called silver.

According to the antibacterial nano silver wound dressing film disclosed by the present invention, the invention also discloses a preparation method of the antibacterial nano silver colloid wound dressing film, wherein the steps 10-18 are preparation of the antibacterial nano silver wound dressing film. The step flow of the method is shown in Figure 1.

In the first diagram of the embodiment of the present invention, first in step 10, an ethanol solution is provided.

Further, in step 12 of the first embodiment of the embodiment of the present invention, polymethacrylic acid is used. 2-Hydroxyethyl ester (pHEMA) was dissolved in an ethanol solution to obtain a poly(2-hydroxyethyl methacrylate) ethanol solution (pHEMA ethanol solution).

Further, in step 14 of the first embodiment of the present invention, the plasticizer, the surfactant, the plurality of nano silver particles and the aqueous solution are uniformly mixed with the poly(2-hydroxyethyl methacrylate) ethanol solution, A mixed viscous liquid is obtained.

In the step 16 of the first embodiment of the present invention, the mixed viscous liquid is subjected to a stretching step to obtain a wet film.

Finally, in step 18 of the first embodiment of the present invention, the ethanol solution remaining in the wet film is removed, whereby the film is fixedly formed.

According to the above procedure, the detailed preparation method of the antibacterial nano silver wound dressing film is as follows: First, in the step 10 shown in Fig. 1, 16 g of polyhydroxyethyl methacrylate was dissolved in a 100 ml ethanol solution. Then, in step 12 of FIG. 1, an appropriate amount of a plasticizer, a surfactant, a plurality of nano silver particles, and an aqueous solution of an aqueous solution of 2-hydroxyethyl methacrylate are uniformly mixed and uniformly mixed. Next, in step 14 of Fig. 1, an aqueous solution containing a plurality of nano silver particles having a volume of 100 ml and a concentration of 2000 ppm is added to the above mixed solution, and thoroughly mixed to form a mixed viscous liquid. It is to be noted that, in the present invention, mixing is carried out using an aqueous solution containing a plurality of nano silver particles, but in order to explain the ratio of the antibacterial nano silver colloid wound dressing film, a plurality of nano silver particles and an aqueous solution are herein. It is considered as two components, but does not affect the material properties of the resulting antibacterial nano silver colloidal wound dressing film and its efficacy.

Continued as in step 16 of Figure 1, using an adjustable wet film applicator (not shown) In the middle of the film, the mixed thick liquid is drawn into a transparent film (not shown) to form a film, and the thickness of the film to be stretched is set to 0.25-0.35 mm (mm) on the adjustable wet film coater. The preferred thickness is 0.3 mm (mm). The film which is stretched at this time is also a wet film, that is, the film contains an ethanol solution. Therefore, according to the step 18 of FIG. 1, the film which has completed the stretching step is placed in a laminar flow hood (not shown) for evaporation and drying. To remove the ethanol solution in the film and to fix the film, where the evaporation is dried for about 12 hours. The film after completely removing the ethanol solution is an antibacterial nano silver colloid wound dressing film having a thickness of 0.05-0.06 mm (mm), preferably 0.06 mm. Here, the ratio of each component in the film is: polyhydroxyethyl methacrylate: plasticizer: surfactant: a plurality of nano silver particles: the ratio of the aqueous solution is 30-50 (W/V%) ): 3-6 (W/V%): 0.3-1.6 (W/V%): 0.1-2 (W/V%): 30-60 (V/V%).

Finally, the antibacterial nano silver colloidal wound dressing film is cut to a specific shape or size according to the desired shape or size, and the antibacterial nano silver colloid wound dressing film is stored in a sterile manner in a sealed envelope.

In the present invention, the physicochemical properties of the antibacterial nano silver colloid wound dressing film can be evaluated, and the prepared antibacterial nano silver wound dressing film can be subjected to water absorption content and water absorption thickness according to the above preparation method and specific ratio. test. First, the water content test was performed by weighing and recording the antibacterial nano silver colloid wound dressing film. Then, the antibacterial nano silver colloid wound dressing film is placed in a vessel filled with water, and the aqueous solution completely covers the entire antibacterial nano silver colloid wound dressing film. After 24 hours, the water-impregnated antibacterial film is further impregnated. The nano silver colloidal wound dressing film was removed to remove surface water droplets and weighed and recorded. The test for water absorption thickness is to measure the thickness of the antibacterial nano silver colloidal wound dressing film at different positions using a thickness gauge and record it. Next, the antibacterial nano silver colloid wound dressing film is placed In a vessel containing water, the aqueous solution is completely covered with the entire antibacterial nano silver colloid wound dressing film, and after 24 hours, the water-impregnated antibacterial nano silver colloid wound dressing film is taken out, and the thickness meter is used. The water-impregnated antibacterial nano silver colloid wound dressing film was then taken out to a thickness of at least three different positions and recorded.

The weight and water absorption of the antibacterial nano silver colloidal wound dressing film before and after water absorption can be obtained from Table 1: Table 2 shows the thickness of the antibacterial nano silver colloid wound dressing film measured at least three different positions before and after water absorption.

It can be obtained from Table 1 and Table 2 that the antibacterial nano silver colloid wound dressing film disclosed in the present invention has good water absorption rate and water swelling rate, and is also attached to the patient. The wound has good water absorption for the liquid (blood or pus) on the wound.

Then, since the antibacterial nano silver colloid wound dressing film is attached to the skin of the human body, the injured part of the patient may be at the joint, and a film having a good tension may be attached to the human skin, so the present invention is directed to The tensile strength test was carried out on the antibacterial nano silver colloid wound dressing film, wherein the tension tension condition was that the film containing the antibacterial nano silver colloid wound dressing was 1 cm in length, and the length after the rupture was stretched from Fig. 2 It is 1.87 cm, so the stretchable length ratio is (1 + 1.87) / 1 = 2.87 times.

In addition, the antibacterial nano silver colloid wound dressing film disclosed in the present invention is also subjected to silver ion release test as shown in FIG. It can be seen from Fig. 3 that the nano silver ions in the antibacterial nano silver colloid wound dressing film are slowly released as the use time is increased to achieve the antibacterial effect.

The above is only the preferred embodiment of the present invention, and is not intended to limit the scope of the present invention; all other equivalent changes or modifications which are not departing from the spirit of the present invention should be included in the following. Within the scope of the patent application.

10‧‧‧ Providing ethanol solution

12‧‧‧ Dissolve 2-hydroxyethyl methacrylate (pHEMA, Poly(2-hydroxyethyl methacrylate)) in ethanol solution to obtain poly(2-hydroxyethyl methacrylate) ethanol solution (pHEMA ethanol solution) )

14‧‧‧Where a plasticizer, a surfactant, a plurality of nano-silver particles and an aqueous solution and a poly(2-hydroxyethyl methacrylate) solution are uniformly mixed to obtain a mixed viscous liquid.

16‧‧‧Draw a mixed viscous liquid for the stretching step to obtain a wet film

18‧‧‧Removing the ethanol solution remaining in the wet film to form a film by fixed molding

Claims (5)

An antibacterial nano silver colloid wound dressing film comprising a biocompatible polymer, a surfactant, a plasticizer, a plurality of nano silver particles and an aqueous solution, comprising: a biocompatible a polymer, wherein the biocompatible polymer comprises poly(2-hydroxyethyl methacrylate), a surfactant, wherein the surfactant is anionic. a surfactant, a cationic surfactant, a nonionic surfactant, and a zwitterionic surfactant group selected; a plasticizer, wherein the plasticizer is ethyl acetoacetate ( Selected from the group of Acetylated monoglyceride, 1,2-Cyclohexane dicarboxylic acid diisononyl ester and Alkyl citrates; a plurality of nano silver And an aqueous solution, wherein the biocompatible polymer, the surfactant, the plasticizer, the plurality of nano silver particles, and the composition ratio of the aqueous solution are 30-50 (W/V%) :3-6 (W/V%): 0.3-1.6 (W/V%) : 0.1-2 (W/V%): 30-60 (V/V%). A method for preparing an antibacterial nano silver colloid wound dressing film, comprising the steps of: providing an ethanol solution; dissolving a poly(2-hydroxyethyl methacrylate) in the ethanol solution to obtain a poly(methacrylic acid) 2-hydroxyl group Ethyl ester ethanol solution; adding a plasticizer, a surfactant, an aqueous solution containing a plurality of nano silver particles and the poly(2-hydroxyethyl methacrylate) ethanol solution are uniformly mixed to obtain a mixed viscous liquid The mixed viscous liquid is subjected to a stretching step to obtain a wet film; and the ethanol solution remaining in the wet film is removed to be fixedly formed to obtain a film. The preparation method of claim 2, wherein the stretching step further comprises: providing a transparent film; placing the mixed viscous liquid on the transparent film; and stretching by using an adjustable wet film coater The mixed viscous liquid on the transparency film is formed to form the wet film having a thickness of 0.25 to 0.35 millimeters (mm). The method of claim 2, wherein the removing the ethanol comprises: placing the wet film in a flow mask; and performing a volatile drying step on the wet film in the laminar flow hood To remove the ethanol solution in the wet film. The preparation method according to claim 2, wherein the film has a thickness of 0.05 to 0.06 mm (mm).