TWI460428B - Side - flow detection device - Google Patents
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- TWI460428B TWI460428B TW101133341A TW101133341A TWI460428B TW I460428 B TWI460428 B TW I460428B TW 101133341 A TW101133341 A TW 101133341A TW 101133341 A TW101133341 A TW 101133341A TW I460428 B TWI460428 B TW I460428B
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- detecting device
- flow detecting
- lateral flow
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- 238000001514 detection method Methods 0.000 title description 5
- 238000012360 testing method Methods 0.000 claims description 21
- 238000010521 absorption reaction Methods 0.000 claims description 13
- 238000001179 sorption measurement Methods 0.000 claims description 11
- 238000003825 pressing Methods 0.000 claims description 5
- 238000000638 solvent extraction Methods 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims 2
- 239000012488 sample solution Substances 0.000 description 8
- 239000000523 sample Substances 0.000 description 7
- 239000011535 reaction buffer Substances 0.000 description 5
- 238000005192 partition Methods 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000000428 dust Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000012491 analyte Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000000984 immunochemical effect Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012125 lateral flow test Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000009597 pregnancy test Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Description
本發明係關於一種檢測裝置,特別是指一種側流式檢測裝置。The present invention relates to a detecting device, and more particularly to a side flow detecting device.
側流式分析(Lateral flow test)又稱作側流式免疫層析法(Lateral flow immunochromatographic assay),係運用毛細管與免疫化學原理,使被分析物在樣本溶液流動的過程中停留在特定的區域,並且呈現出肉眼可見的顏色,藉此快速得知樣本中是否存在有被分析物。此一技術自1988年被商品化成為驗孕棒之後,由於具有使用方便、可快速獲得檢驗結果、穩定性高可長期保存,以及製造成本低等優點,現已廣泛應用在臨床醫學、獸醫、農業、食品工業、環境檢測等各種不同領域。Lateral flow test, also known as Lateral flow immunochromatographic assay, uses capillary and immunochemical principles to allow analytes to stay in specific areas during sample solution flow. And presenting a color visible to the naked eye, thereby quickly knowing whether or not an analyte is present in the sample. This technology has been widely used in clinical medicine, veterinary medicine, since it was commercialized into a pregnancy test stick in 1988. It has the advantages of convenient use, quick test results, high stability, long-term preservation, and low manufacturing cost. Agriculture, food industry, environmental testing and other different fields.
如台灣公開第201013186號專利中所示,一般的側流式檢測裝置具有一個結果視窗以及一個樣本添加孔,使用時先將樣本與反應緩衝液混合,再將混合之後的樣本溶液滴入樣本添加孔中反應,便可從結果視窗中判讀檢驗結果。但是前述側流式檢測裝置需要搭配其他採檢和混合工具,例如棉花棒、滴管、試管等等,因此造成使用上的不便利。As shown in Taiwan Patent Publication No. 201013186, a general lateral flow detecting device has a result window and a sample adding hole. When used, the sample is mixed with the reaction buffer, and the mixed sample solution is dropped into the sample. The reaction in the well allows the test results to be interpreted from the results window. However, the aforementioned lateral flow detecting device needs to be combined with other inspection and mixing tools, such as cotton swabs, droppers, test tubes, etc., thus causing inconvenience in use.
本發明之主要目的在於提供一種側流式檢測裝置,其具有較佳的使用便利性。SUMMARY OF THE INVENTION A primary object of the present invention is to provide a sidestream type detecting device which has better usability.
為達成前述目的,本發明所提供之側流式檢測裝置,包含有一本體、一試條、一固定座、一擦拭件以及一蓋件。其中該本體具有一連接部以及一表面,該連接部開設有一開口,該表面開設有一視窗,並且該本體內設有一第一腔室,該第一腔室連通該開口與該視窗;該試條設置於該第一腔室,且該試條具有一吸收區以及一結果顯示區,該吸收區伸出該開口,該結果顯示區對應設置於該視窗開設處;該固定座具有一第二腔室以及一第三腔室,該連接部可分離地設於該第二腔室,該第二腔室與該第三腔室之間具有一分隔件,該分隔件開設有一穿孔,該吸收區穿過該穿孔而伸入該第三腔室;該擦拭件具有一接合部以及一吸附部,該接合部可分離地設於該第三腔室並與該吸收區相互接合,該吸附部凸伸出該第三腔室;該蓋件可分離地蓋合於該固定座,且該蓋件具有一第四腔室,該吸附部設於該第四腔室。To achieve the foregoing objective, the lateral flow detecting device provided by the present invention comprises a body, a test strip, a fixing seat, a wiping member and a cover member. The main body has a connecting portion and a surface, the connecting portion defines an opening, the surface defines a window, and the body is provided with a first chamber, the first chamber communicates with the opening and the window; the test strip Provided in the first chamber, the test strip has an absorption area and a result display area, the absorption area extends out of the opening, and the result display area is correspondingly disposed at the opening of the window; the fixing seat has a second cavity And a third chamber, the connecting portion is detachably disposed in the second chamber, and a partition is disposed between the second chamber and the third chamber, the partition is provided with a perforation, the absorption region And extending into the third chamber through the through hole; the wiping member has a joint portion and a suction portion detachably provided in the third chamber and engaged with the absorption region, the adsorption portion is convex Extending the third chamber; the cover member is detachably attached to the fixing seat, and the cover member has a fourth chamber, and the adsorption portion is disposed in the fourth chamber.
藉由上述技術特徵,本發明所提供之側流式檢測裝置可利用該擦拭件擦拭欲檢測的地方,使樣本附著在該吸附部後,與滴入該第四腔室中之反應緩衝液混合,以進行後續反應,因此可簡化檢測的流程與步驟,而達到較佳的使用便利性。According to the above technical feature, the lateral flow detecting device provided by the present invention can wipe the place to be detected by the wiping member, and after the sample is attached to the adsorption portion, mix with the reaction buffer dropped into the fourth chamber. In order to carry out the subsequent reaction, the flow and the steps of the detection can be simplified, and the convenience of use is achieved.
有關本發明之詳細構造、特點、組裝方式或使用方式,將於後續之詳細說明中予以描述。然而,在本發明領域中 具有通常知識者應能瞭解,該等詳細說明以及實施本發明所列舉之特定實施例,僅用於說明本發明,並非用以限制本發明之專利申請範圍。Detailed construction, features, assembly or use of the present invention will be described in the detailed description that follows. However, in the field of the invention The detailed description and specific examples of the invention are intended to be illustrative of the invention and are not intended to limit the scope of the invention.
以下將藉由所列舉之實施例,配合隨附之圖式,詳細說明本發明之技術內容及特徵。The technical contents and features of the present invention will be described in detail below with reference to the accompanying drawings.
如第一圖至第三圖所示,本發明一較佳實施例所揭露之側流式檢測裝置10主要包含有一本體20、一試條30、一固定座40、一擦拭件50以及一蓋件60。As shown in the first to third embodiments, a lateral flow detecting device 10 according to a preferred embodiment of the present invention mainly includes a body 20, a test strip 30, a fixing base 40, a wiping member 50, and a cover. Item 60.
該本體20概呈長矩形,其一端具有一連接部202,一側具有一表面204,該表面204則開設有一視窗206。如第三圖所示,該本體20內設有一第一腔室208,用以設置該試條30,該第一腔室208連通該視窗206。The body 20 has a long rectangular shape with a connecting portion 202 at one end and a surface 204 on one side. The surface 204 defines a window 206. As shown in the third figure, a first chamber 208 is disposed in the body 20 for setting the test strip 30, and the first chamber 208 is connected to the window 206.
進一步言之,該本體20係由一上蓋22、一下蓋24以及一結合手段26所組成。該視窗206設置於該上蓋22,用以觀察該試條30的檢測結果。該下蓋24具有一承載台242,該承載台242係由至少一個邊框單元244以及至少一個第一支撐肋246所組成,如本實施例第三圖所示,可由五個邊框單元244形成邊框,並且由六條第一支撐肋246相互垂直交錯組成底部,而組成一個突出於該下蓋24底面的長矩形區域。該結合手段26則包含有複數個環繞設置於該上蓋22邊緣的凸柱222,以及複數個對應於該凸柱222而設置於該下蓋24的插孔248,藉此結合該上蓋22與該 下蓋24。該上蓋22更設置有至少一個抵壓單元224,該抵壓單元224朝該等第一支撐肋246方向延伸而突出於該上蓋22底面,如本實施例第三圖所示,可設置有三個抵壓單元224。Further, the body 20 is composed of an upper cover 22, a lower cover 24 and a coupling means 26. The window 206 is disposed on the upper cover 22 for observing the detection result of the test strip 30. The lower cover 24 has a loading platform 242 which is composed of at least one frame unit 244 and at least one first supporting rib 246. As shown in the third embodiment of the embodiment, the frame can be formed by five frame units 244. And the six first support ribs 246 are vertically staggered to form a bottom portion, and constitute a long rectangular area protruding from the bottom surface of the lower cover 24. The bonding means 26 includes a plurality of protrusions 222 disposed around the edge of the upper cover 22, and a plurality of insertion holes 248 corresponding to the protrusions 222 and disposed on the lower cover 24, thereby combining the upper cover 22 and the Lower cover 24. The upper cover 22 is further provided with at least one pressing unit 224 extending toward the first supporting ribs 246 to protrude from the bottom surface of the upper cover 22, as shown in the third embodiment of the embodiment, three The unit 224 is pressed.
如第三圖與第四圖所示,該試條30為應用側流式分析技術或免疫層析法的檢測試條,其設置於該第一腔室208中,並且放置在該承載台242上。當該上蓋22與該下蓋24結合時,藉由該抵壓單元224的壓迫,可使該試條30穩固地定位於該承載台242上。再者,該試條30具有一結果顯示區32以及一吸收區34,該結果顯示區32則對應設置於該視窗206開設處,藉此,如第一圖所示,透過該視窗206即可從該本體20外部直接看到該結果顯示區32。As shown in the third and fourth figures, the test strip 30 is a test strip using a lateral flow analysis technique or an immunochromatography, which is disposed in the first chamber 208 and placed on the carrier 242. on. When the upper cover 22 is combined with the lower cover 24, the test strip 30 can be stably positioned on the support base 242 by the pressing of the pressing unit 224. Furthermore, the test strip 30 has a result display area 32 and an absorption area 34, and the result display area 32 is correspondingly disposed at the opening of the window 206, thereby being visible through the window 206 as shown in the first figure. The result display area 32 is directly seen from outside the body 20.
如第二圖與第四圖所示,該固定座40係可分離地設於該本體20,其具有一第二腔室402以及一第三腔室404,該連接部202可分離地設於該第二腔室402,該第二腔室402與該第三腔室404之間具有一分隔件42,該分隔件42開設有一穿孔422,該吸收區34穿過該穿孔422而伸入該第三腔室404。其中,該連接部202具有二相對設置的凸肋28,該第二腔室402之壁面亦相對設置有二長槽44,該等凸肋28與該等長槽44相互對應,藉此使該等凸肋28與該等長槽44相互嵌合,讓該連接部202更穩固地設於該第二腔室402而不易分離。再者,該固定座40更具有至少一第二支撐肋46,該第二支撐肋46由該分隔件42與該第三腔室404之壁面朝該第三腔室404延伸而出,以承置該 吸收區34。As shown in the second and fourth figures, the fixing base 40 is detachably disposed on the body 20, and has a second chamber 402 and a third chamber 404. The connecting portion 202 is detachably disposed on the second portion. The second chamber 402 has a partition 42 between the second chamber 402 and the third chamber 404. The partition 42 defines a through hole 422. The absorbing area 34 extends through the through hole 422. The third chamber 404. The connecting portion 202 has two opposite ribs 28, and the wall surface of the second chamber 402 is oppositely disposed with two long slots 44, and the ribs 28 and the long slots 44 correspond to each other, thereby The equal ribs 28 and the equal length grooves 44 are fitted to each other, so that the connecting portion 202 is more stably disposed in the second chamber 402 without being easily separated. Furthermore, the fixing base 40 further has at least one second supporting rib 46 extending from the wall of the partitioning member 42 and the third chamber 404 toward the third chamber 404 for receiving Set this Absorption zone 34.
如第二圖與第四圖所示,該擦拭件50具有一接合部52以及一吸附部54,該接合部52可分離地設於該第三腔室404並且與該吸收區34相互接合,使樣本溶液可由該擦拭件50流至該試條30,該吸附部54凸伸出該第三腔室404。其中,該擦拭件50係由多孔並具有相當硬度之材質所製成,例如泡綿、毛氈纖維等等,使得樣本溶液可透過毛細作用被該擦拭件50所吸入,並且可將如灰塵、皮屑、泥土等等粉塵狀,或是其他形態的微量樣本,透過擦拭的方式吸附在該擦拭件50上,並且擦拭的過程中不易因為擠壓而變形。進一步而言,該接合部52具有一接觸面522,該接觸面522與該吸收區34相互貼合,以增加該擦拭件50與該試條30之間的接觸面積,以增進樣本溶液在該擦拭件50與該試條30之間流動的效率。As shown in the second and fourth figures, the wiper 50 has a joint portion 52 and a suction portion 54 detachably provided to the third chamber 404 and engaged with the absorption region 34. The sample solution can be caused to flow from the wiper 50 to the test strip 30, and the adsorption portion 54 projects out of the third chamber 404. Wherein, the wiping member 50 is made of a material that is porous and has a relatively high hardness, such as foam, felt fiber, etc., so that the sample solution can be inhaled by the wiping member 50 through capillary action, and can be like dust, skin. Dust, dirt, and the like, or other forms of micro-samples, are adsorbed on the wiper 50 by wiping, and are not easily deformed by extrusion during wiping. Further, the joint portion 52 has a contact surface 522, and the contact surface 522 and the absorption region 34 are adhered to each other to increase the contact area between the wiper 50 and the test strip 30 to enhance the sample solution. The efficiency of flow between the wiper 50 and the test strip 30.
該蓋件60具有一第四腔室62,並且該蓋件60可分離地蓋合於該固定座40。當該蓋件60蓋合於該固定座40時,該第三腔室404與該第四腔室62呈密封狀態,且該吸附部54設於該第四腔室62內。The cover member 60 has a fourth chamber 62, and the cover member 60 is detachably attached to the mount 40. When the cover member 60 is closed to the fixing base 40 , the third chamber 404 and the fourth chamber 62 are sealed, and the adsorption portion 54 is disposed in the fourth chamber 62 .
使用本發明所提供的側流式檢測裝置10時,可利用該擦拭件50的該吸附部54在所欲檢測的地方來回擦拭,使樣本附著在該吸附部54。接著,如第四圖所示,將反應緩衝液滴入該蓋件60的該第四腔室62中後,把該蓋件60與該固定座40組合,使該吸附部54浸入反應緩衝液之中,讓樣本與反應緩衝液混合成為樣本溶液S。樣本溶液S透 過毛細作用,依序流過該接合部52、該吸收區34以及該結果顯示區42,以進行後續分析反應。因此,在使用的過程中可以簡化整個檢測的流程與步驟,不需要再搭配其他採檢和混合工具,例如棉花棒、滴管、試管等等,而可達到較佳的使用便利性。When the lateral flow type detecting device 10 according to the present invention is used, the suction portion 54 of the wiper 50 can be wiped back and forth at a place to be detected to adhere the sample to the adsorption portion 54. Next, as shown in the fourth figure, after the reaction buffer is dropped into the fourth chamber 62 of the cover member 60, the cover member 60 is combined with the fixing base 40, and the adsorption portion 54 is immersed in the reaction buffer. Among them, the sample is mixed with the reaction buffer to form a sample solution S. Sample solution S Through capillary action, the joint portion 52, the absorption region 34, and the result display region 42 are sequentially flowed for subsequent analysis reactions. Therefore, the process and steps of the entire inspection can be simplified in the process of use, and it is not necessary to cooperate with other inspection and mixing tools, such as cotton swabs, droppers, test tubes, etc., to achieve better usability.
在此必須說明者為,以上配合圖式所為之詳細描述,僅係為了說明本發明之技術內容及特徵而提供之一實施方式,凡在本發明領域中具有一般通常知識之人,在瞭解本發明之技術內容及特徵之後,於不違背本發明之精神下,所為之種種簡單之修飾、替換或構件之減省,皆應屬於以下所揭示之申請專利範圍之內。It is to be understood that the foregoing detailed description of the drawings is merely illustrative of the technical aspects and features of the present invention, and those who have ordinary knowledge in the field of the invention </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt; </ RTI> <RTIgt;
10‧‧‧側流式檢測裝置10‧‧‧Side flow detection device
20‧‧‧本體20‧‧‧ body
202‧‧‧連接部202‧‧‧Connecting Department
204‧‧‧表面204‧‧‧ surface
206‧‧‧視窗206‧‧‧Window
208‧‧‧第一腔室208‧‧‧First chamber
22‧‧‧上蓋22‧‧‧Upper cover
222‧‧‧凸柱222‧‧‧Bump
224‧‧‧抵壓單元224‧‧‧Compression unit
24‧‧‧下蓋24‧‧‧Under the cover
242‧‧‧承載台242‧‧‧Loading station
244‧‧‧邊框單元244‧‧‧Border unit
246‧‧‧第一支撐肋246‧‧‧First support rib
248‧‧‧插孔248‧‧‧ jack
26‧‧‧結合手段26‧‧‧Combined means
30‧‧‧試條30‧‧‧Test strips
32‧‧‧結果顯示區32‧‧‧ Results display area
34‧‧‧吸收區34‧‧‧Absorption zone
40‧‧‧固定座40‧‧‧ Fixed seat
402‧‧‧第二腔室402‧‧‧Second chamber
404‧‧‧第三腔室404‧‧‧ third chamber
42‧‧‧分隔件42‧‧‧Parts
422‧‧‧穿孔422‧‧‧Perforation
44‧‧‧長槽44‧‧‧Long slot
46‧‧‧第二支撐肋46‧‧‧second support rib
50‧‧‧擦拭件50‧‧‧ wipes
52‧‧‧接合部52‧‧‧ joints
54‧‧‧吸附部54‧‧‧Adsorption Department
60‧‧‧蓋件60‧‧‧Cleaning pieces
62‧‧‧第四腔室62‧‧‧fourth chamber
S‧‧‧樣本溶液S‧‧‧ sample solution
第一圖係本發明一較佳實施例之立體視圖;第二圖係本發明該較佳實施例之立體分解圖以及部份元件之部份剖示圖;第三圖係本發明該較佳實施例中部份元件之立體分解圖;以及第四圖係本發明該較佳實施例中第一圖之4-4剖面圖,並且顯示本發明於使用時之狀態。The first drawing is a perspective view of a preferred embodiment of the present invention; the second drawing is a perspective exploded view of the preferred embodiment of the present invention and a partial cross-sectional view of some of the components; An exploded perspective view of a portion of the components in the embodiment; and a fourth section is a cross-sectional view of the first embodiment of the preferred embodiment of the present invention, taken along line 4-4, and showing the state of the invention in use.
10‧‧‧側流式檢測裝置10‧‧‧Side flow detection device
20‧‧‧本體20‧‧‧ body
206‧‧‧視窗206‧‧‧Window
32‧‧‧結果顯示區32‧‧‧ Results display area
60‧‧‧蓋件60‧‧‧Cleaning pieces
Claims (9)
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TW101133341A TWI460428B (en) | 2012-09-12 | 2012-09-12 | Side - flow detection device |
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TW101133341A TWI460428B (en) | 2012-09-12 | 2012-09-12 | Side - flow detection device |
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TW201411129A TW201411129A (en) | 2014-03-16 |
TWI460428B true TWI460428B (en) | 2014-11-11 |
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TW101133341A TWI460428B (en) | 2012-09-12 | 2012-09-12 | Side - flow detection device |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI699534B (en) * | 2017-03-14 | 2020-07-21 | 日商電化生研股份有限公司 | Immunochromatography device for extracting and measuring sugar chain antigen, and method for measuring sugar chain antigen in sample |
TWI699533B (en) * | 2017-03-14 | 2020-07-21 | 日商電化生研股份有限公司 | Prevents non-specific reactions, immunochromatographic test strips for extracting and measuring sugar chain antigens, and methods for measuring sugar chain antigens in samples |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102460185A (en) * | 2009-06-19 | 2012-05-16 | Zbx公司 | Hinged cap for diagnostic device |
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CN102460185A (en) * | 2009-06-19 | 2012-05-16 | Zbx公司 | Hinged cap for diagnostic device |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI699534B (en) * | 2017-03-14 | 2020-07-21 | 日商電化生研股份有限公司 | Immunochromatography device for extracting and measuring sugar chain antigen, and method for measuring sugar chain antigen in sample |
TWI699533B (en) * | 2017-03-14 | 2020-07-21 | 日商電化生研股份有限公司 | Prevents non-specific reactions, immunochromatographic test strips for extracting and measuring sugar chain antigens, and methods for measuring sugar chain antigens in samples |
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TW201411129A (en) | 2014-03-16 |
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