TWI312008B - Human analogs of murine deubiquitinating protease genes - Google Patents

Description

1312008

V. INSTRUCTIONS (1) MLt_S_ From the biochemical study of cell-free systems of reticulocytes, the role of ubiquitin in protein degradation and the major enzyme reactions of this system are described. In this system, proteins are induced to undergo degradation by covalent attachment to a pancreatic hormone (a protein having 76 5 amino acid residues). In short, ubiquitin-protein junctions require two enzymes to act in succession. In the step (step 丨) in which ATP is required to utilize specific activator (E1), the c-terminal Gly residue of the pancreatic hormone is activated. This step involves the formation of an intermediate product of pan-hormone adenosine, which releases PPi, which is then combined with the CyS residue of E1 by a thiol bond to release AMP. The activated pancreatic hormone is then transferred to the live recovery site of the ubiquitin-carrier protein (E2) Cys residue (step 2). In the third step of ubiquitin-protein ligase or E3 enzyme (in the third step, the pan-hormone is linked via its C-terminus to the amine group of the residue of the matrix protein via the indoleamine peptide (steps) 3) 15 Proteins that bind to the polyubiquitin chain are usually degraded by the 26S proteasome, the Ministry of Economic Affairs, the Intellectual Property Bureau, the Consumer Cooperative, and the ATP hydrolysis. The 26S proteasome is composed of ATP containing 2〇s proteasome. The dependent assembly is formed, and the 20S proteasome is a complex containing a protease containing catalytic site of 19S "cap" or a regulatory complex. The 19S complex contains several ATPase subunits and other subunits, 20 of which are presumed to be Involved in the specific role of the 26S proteasome on ubiquitinated proteins. The role of ATP in the 26S proteasome complex and its proteolysis is not known. The role of the 26S proteasome is presumed to produce several products. : the free peptide, the short peptide that is still linked to the pancreatic hormone via its LyS residue, and the polyubiquitin chain (step 4). The paper size applies to the Chinese National Standard (CNS) A4 specification (210). X 297 mm) 1312008 V. Description of the invention (2) The effect of the second product, the sputum hormone _C terminal water _ or the isozyme, is free and reproducible (steps 5 and 6). Decompose a specific pan-hormone egg from the f-combination (step 7), and thus it is proteolyzed by the 26S proteasome. The post-type heterozygous enzyme may have a regulatory function that may have the ability to rescue the erroneously ubiquitinated protein. Role. The short peptide formed by the above process can be further degraded into amino acid by cytosolic peptide (step 8). 'The Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed protein degradation induced by pancreatic hormone Multiple biological programs. Selective and programmed degradation of cell cycle regulatory proteins (eg, cyclin, cyclin-dependent kinase inhibition, and post-inhibition (4)) are essential for cell cycle progression. Cell growth and proliferation using tumors Inhibition of genes, proto-oncogenes, and signal transduction system components are controlled by ubiquitin-mediated protein degradation. The rapid degradation of many transcriptional regulators involves various signaling processes and Response to environmental causes. The ubiquitin system is clearly involved in the phagocytosis and downregulation of receptors and 15 transporters, as well as in the degradation of resident or abnormal proteins in the endoplasmic reticulum. There are strong indications of ubiquitin system development and cells. The role of apoptosis, but the target proteins involved have not yet been identified. Several dysfunctions of the ubiquitin-transfer procedure lead to pathological conditions, including malignant lesions. 20 My differences in the use of proteins for labeling ubiquitination The understanding of the message is also limited. Recent reports indicate that 'many proteins are guided to degrade by phosphorylation. Many rapidly degraded proteins have been previously observed to contain PEST elements' which are regions rich in Pro, Glu, Ser, and Thr residues. Recently, it has been reported that the PEST component is rich in s/TP sequences. The paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm).

, invention description 5 10 15 Ministry of Economic Affairs Intellectual Property Officer X Consumer Cooperatives printed 20 CCdks and some other eggs from the smallest kinase - _ acidification bit p = = if (of course not all) examples, it seems now ^ 3 has a scalar site required for degradation. Therefore, multiple phosphorylation in the PEST element is required for the yeast (7) cyclin (f) and the coffee, and the Gen4 ride (four) for the cyclization. Other proteins:, for example, the cyclin cyclin cyclin and cyclin 01, eutrophic; the specific I site of Weihuaxian (four) material to produce ubiquitination. In the case of a coffee inhibitor of the NF-kB transcriptional regulator, the two specific sites of (6) 2 and Sef_36 are required for acidification to be ubiquitin. The effect of Xiang Guhua is expected to produce a ubiquitin-mediated degradation of the f-chain (10) rhyme emln) ′ in their Weihua position, which has a similar (4) sequence structure. However, the homogeneity of the acidification pattern of this diprotein (IV) is not complete, because the degradation of the f-chain is still required for its mechanization at other sites. Other proteins that are degraded by the genus include the Cdk inhibitors Siclp and the STAT1 transcript. Although the (four) Wei-dip article has degraded from f, the common feature seems to be the initial project by protein kinase, and the role of pan-hormone synthase is to identify the phosphorylated form of the protein matrix. Into the ground, it seems that different ubiquitin ligases recognize different acidification patterns of the dish, as well as additional major structures in various protein matrices. However, in addition to the role of germination in the yeast cell cycle regulation _ ρυιχ type ubiquitin ligase, the similarity of these E3 is unknown. It has been observed that some protein-acidification does prevent its degradation, and it is believed that the message that induces the degradation of ubiquitin-mediated ubiquitin secretion (and the need to recognize such information - ς _ is applicable to this paper scale) The diversity of the Chinese National Standard (CNS) A4 specification (A7 B7 1312008 V, invention description (4) ligase) has attracted attention. Thus, the scalarization of CM〇s proto-oncogene on Ser3, and c-Fos and c- Multiple sites on the Jun proto-oncogene use multiple phosphorylation of MAP kinase to inhibit ubiquitination and degradation. 5 In addition to the family of enzymes involved in ubiquitin combination, there have recently been intellectual property from various bioeconomic sectors. The Bureau of Employees' Consumer Cooperatives has identified a large family of ubiquitinated enzymes. These enzymes have several possible functions. First, they have a peptide activity and a cleavable pancreatic hormone gene product. A different gene encoding, which is a polyubiquitin gene, which encodes a ubiquitin linear polymer linked via a peptide bond between the C-terminus Gly of the ubiquitin molecule and the N-terminal 10 Met. The release of ubiquitin must accurately cleave the peptide bond between successive ubiquitin groups. Another type of ubiquitin gene encodes a ubiquitin c-terminal extension protein, which is the peptide bond between the ubiquitin C-terminal Gly and the extended protein N-terminal Met. Fusions. To date, the extensions are ribosomal proteins consisting of 52 or 76-8 amino acids 15. Their pan-hormone fusion proteins are processed to obtain ubiquitin and corresponding C-terminal extension proteins. The ubiquitinating enzyme may have isokinase activity. When the target protein is degraded, the ubiquitinating enzyme cleaves the polyubiquitin chain from the target protein or its residue. During or after proteolysis by the 26 S proteasome, The polyubiquitin chain 20 must also be cleaved by the ubiquitinated enzyme to produce free ubiquinone monomers. In this way, the ubiquitinating enzyme promotes the ability of the 26 s proteasome to degrade the ubiquitinated protein. , a ubiquitinating enzyme hydrolysable glucosamine Gly-76 carboxyl ester, thiol ester, and guanamine linkage. These non-functional linkages may be composed of small intracellular compounds (eg Glutathione) -6- present paper and applies the Chinese national standard scale (CNS) A4 size - " --- 1312008 V. invention is described in (5

The reaction between the El-, E2-, or E3_ panthenol thiol ester intermediates was produced. Fourth, de- gonadotropin may rescue the hormonal competition from the protein matrix to protect it from degradation or any other function by ubiquitination. Therefore, the use of deubiquitinating enzymes to produce pan-hormone from bi-type polyubiquitin and pan-hormone fusion proteins and self-binding to the branching polyglycols of proteins is very important for maintaining a sufficient amount of free ubiquitin. Many deubiquitinating enzymes exist, suggesting that they are ubiquitinating enzymes to recognize different matrices' and thus involve specific cellular processes. Although there are recent evidences such as support for serotonin-like enzymes, there is still little research on the structural and functional association of enzymes such as 10 . The Department of Economic Intelligence's Intellectual Property Bureau employees' consumption cooperatives print ubiquitinated enzymes, which can be broadly classified into two types according to sequence homogeneity. UBP or USP, also known as type 2 ubiquitin c-terminal hydrolase (type 2) UCH)], and UCH, is also called type 1 uCH. The UCH (UUCH) enzyme mainly hydrolyzes the ubiquitin hormone and the guanamine, but also cuts the pancreatic hormone gene product and disassembles the polyubiquitin chain. It typically has a 210-amino acid catalytic functional moiety' with four highly conserved sequence inserts confirming these enzymes. UCH Enzyme contains two very conservative main structures, CYS and HIS E. Mutation formation studies have shown that this two plays a key role in the catalytic action of some UCH enzymes with significant C-terminal extensions. The function of the C-extension is still unknown, but it seems to involve proper positioning of the enzyme. The active sites of these UCH enzymes contain a catalytic three-reading consisting of cysteine, histidine, and aspartame, and the chemical mechanism of papaya-like enzymes. The crystal structure of one of the ' UCH_U' is resolved with a resolution of 1.8A. This enzyme contains a central anti-parallel stone-plane. This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm). 1312008

(cold-sheet) with side-by-side spiral chains on both sides. The point-plane and the helix chain are similar to those observed for the thiol protease cathepsin B, and the similarities include the three amino acid residues constituting the active site, Cys95, His169, and Asp184. This active site appears to be suitable for pan- 5 hormone binding that can also be fixed at other sites. The catalytic site in the free enzyme is masked by two different segments of the molecule' to limit non-specific hydrolysis, so after the matrix is combined, a configuration rearrangement must be performed. The UBP (type 2 UCH) enzyme cleaves the pancreatic hormone gene product and disassembles the polyubiquitin chain after hydrolysis. It appears to be present in the core region of about 40 amino acids limited by CYS and HIS. There are many N-terminal extensions and some C-terminal extensions. In addition, many of the core areas of the same type have various sequences. The functions of their divergent sequences have not been confirmed. Another interesting feature of specific UBP is the regulation of cell proliferation. Studies have observed that cytokines induce T-cell-specific deubiquitinating enzyme 15 (DUB), called DUB-1 and DUB-2. DUB-1 is induced by stimulating IL-3, IL-Ministry of Finance, Intellectual Property, and Consumer Cooperatives to print 5' and GM-CSF cytokine receptors, suggesting that it is a common third in the induction of interleukin receptors. The role in the unit (betac). A large number of JAK2 dominant negative mutants inhibit the cytokine induction of DUB-1, suggesting that regulation of this enzyme is part of the cellular response to the 20 JAK/STAT message transduction pathway. The continued performance of DUB-1 inhibits cells from G!; therefore, the enzyme appears to regulate cell growth by controlling Go-G! transfer. The catalytically conjugated Cys residue of this enzyme is required for its 'activity. DUB-2 is induced by IL-2 and is a very early (IE) gene that is regulated downwards shortly after the onset of stimulation. The function of this enzyme is not applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 mm). Α7 Β7 1312008 V. Description of the invention (7) Clear 'may stimulate or inhibit the degradation of important cell cycle regulators. Cytokines, such as interleukin-2 (TL-2), activate intracellular signaling via rapid lysine nucleolation of their receptors, resulting in the activation of many genes involved in cell growth and survival. Deubiquitinating enzyme DUB-2 is induced in response to IL-5 2 and exhibits 'express IL-2 JAK/STAT' in human T-cell lymphovirus_丨 (HTLV-1) transfected T cells. Activation of the composition of the message transducer and transcriptional activator pathways, DUB-2 significantly prolonged IL-2-induced STAT5 sclerotation when expressed in Ba/F3 cells. Although DUB-2 does not increase the proliferation of IL-2-mediated, it is a STAT5 phosphorylation of the cell branch of DUB-2 and enhances IL-2-induced genes when extracted from growth factors. The performance of DUB-2 significantly inhibits the apoptosis induced by the detachment of cytokines and allows the cells to survive. Therefore, DUB-2 plays a role in promoting the signaling through the JAK/STAT pathway and prolonging the survival of lymphocytes, and When constitutively expressed 15, it may contribute to the activation of the JAK/STAT pathway observed in some transformed cells (Migone, T.-S., et al., Blood. 2001; 98: 1935-1941) The Department of Economic Intelligence's Intellectual Property Bureau employees' consumption cooperatives prints protein ubiquitination as a signal transduction pathway for cytokine activation and an important regulator of hematopoietic cell growth. This effect is regulated by ubiquitin and cytokines. Controlled by the coordination role. Recently, a new gene encoding the ubiquitin-producing enzymes (DUB-1 and DUB-2) that regulate growth has been identified. It is a very early gene that is rapidly and transiently stimulated by cytokines. Ground induction Chain reaction, amplification of complementary DNA by degenerate primer, single out of the sequence containing the gene. The paper scale is applicable to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Inventive Note (8) A murine bacterial artificial chromosome (BAC) transgenic strain. A BAC contains a novel DUB gene (DUB-2/4) that has itch-ubiquity homology with DUB-2, and is (7)?-/ and, similarly, the /Χ The /β-Μ gene consists of two expression sequences. The expected DUB-2 protein is highly correlated with other DUBs in terms of the entire major amino acid sequence, and is highly variable at the C-terminus. Ι) ί/β-2/ has functional deubiquitination activity; mutations of its conserved amino acid residues completely destroy this activity. The 5' flanking sequence of the gene has a hematopoietic-specific functional promoter sequence. It has been suggested in the literature that at least three members of the subfamily 10 2 and D(10)-Μ)' and different hematopoietic cytokines induce specific genes to initiate cytokine-specific growth responses (Baek, K.-H., et al. , Blood. 2001; 98: 636-642). Protein ubiquitination also supplies regulatory functions in the cell that do not involve degradation by proteasome transfer. For example, 'Hicke and Riezman recently demonstrated that ligand-induced ubiquitination of the Ste2 receptor in yeast. The ubiquitination of the Ste2 receptor triggers receptor phagocytosis and directs the receptor to the vacuole, not the proteasome. At the same time, Chen et al. have demonstrated that the activation of the enzymes produced by the IB Ministry of Economics and Intellectual Property Office staff consumption cooperatives requires a rapid, inflammatory-induced ubiquitination project. This ubiquitination project is essential for IB specific phosphorylation without the subsequent proteolysis of the kinase 20 complex. The ubiquitination of Ste2 and ffi kinase appears to be reversible, perhaps due to the action of specific ubiquitinating enzymes. Recently, the gene encoding the ubiquitinated enzyme (or UBps), which has a super large family, has been set. UBPs are ubiquitin-specific thiol-proteases, which cleave linear ubiquitin precursor proteins or isopeptide hormonal complexes -10- °. This paper scale applies to Chinese National Standard (CNS) A4 specifications (210 X 297 mm). );12008

V. Description of the invention (Ο Modified protein after translation. A large number of UBPs suggest that protein ubiquitination, like protein scurification, is a highly reversible procedure that is regulated in cells. 5 Interestingly, UBPs There is a great difference in length and structural complexity, suggesting a diversity of functions. While the similarity of the amino acid sequence in the entire coding region is minimal, two conserved functional sites are shown by sequence comparison: Cys functional site contains cysteamine An acid residue that has the action of an active enzyme nucleophilic substance; His functional site contains a histidine residue that contributes to the active site of the enzyme. More recent evidence proves that all members of the ubp superfamily contain six homogeneities. The functional site. The mutations formed by the conserved residues of the Cys and His functional sites have identified several residues necessary for UBP activity. Recently, the growth-regulating ubiquitin-like enzymes that are stimulated by the cytokine receptor and are induced by the stimuli, , was identified. It was induced by Jiang-3 (granulocyte macrophage_colony_stimulating factor) and specific receptors of _5. The specific angle of the c-unit shared by the body 2. In the process of gene transfer, a cross of a related family and a cross (10) gene are identified. It can be seen that other genes may be induced by different growth factors. The (10) enzyme, which is induced by IL_2, and the closely related DUB-2a, were identified as being more related to each other than the other members of the 20 ubp superfamily, thereby defining a novel subfamily of ubiquitinated enzymes. DUBs with hematopoietic specificity induced by cytokines have been shown to prolong cytokine receptors, see Mig〇ne, T $ (2001). Deubiquitinating enzyme DUB_2 prolongs cytokine induction-11-1312008 A7 B7 V. INSTRUCTIONS (10) Reagents and activators of transcriptional activation and inhibition of apoptosis after cytokine withdrawal [Blood 98, 1935-41; Zhu, Y., et al (1997). DUB_2 A member of a new family of ubiquitin-producing enzymes that can be induced by cytokines, J Biol Chem 272, 51-7 and Zhu, Y., et 5 (1996). The murine DUB-丨 gene is composed of interleukin-3 receptor. Betac subunit-specific induction, Mol Cell Biol M , 4808_17). These effects may be due to the deubiquitination of receptors or other signaling intermediates by the DUB-1 or DUB-2, a murine analog of hDUBs. Inhibition of hDUBs may result in down-regulation of specific cytokine receptor signaling, thereby regulating specific plague responses. Cytokines regulate cell growth by inducing the expression of specific targets. A very early gene identified by cytokines, recently identified, encodes a ubiquitinated enzyme with growth-regulating activity. In addition, a highly related gene, D(10)_2, induced by interleukin-2 was also identified. 15 D(10)-2 mRNA is induced in very early genes in T cells and is rapidly regulated downward. Similarly, the £)t/5-2 protein has deubiquitination activity in vitro. When the pan-hormone-specific thiol protease activity is required by the Ministry of Economic Affairs, the Intellectual Property Office, the Consumer Cooperative, to print the D(10)-2 conserved cysteine residue to become a serine (C6〇s), the ubiquitination activity is completely damage. D05-/ has a high correlation with the entire major amino acid sequence except for the highly variable region of its c〇〇h 20 terminus. In addition, the genes are co-localized in the region of mouse smear 7, suggesting that they are produced by tandem replication of ancestral genes. Other public (10) genes are also co-localized in this region, indicating the presence of a large family of cytokines that are induced by cytokines. We propose (4) the auxin-induced specific -12- '1312008 5, the invention (11) gene, the induced iX/5 enzyme to regulate the degradation or ubiquitination of an unknown growth regulator' cytokines Specific growth response. According to these structural guidelines, other members of the subfamily can be identified in GenBankTM. The highest degree of homogeneity is in the cys and His function. At the same time, this putative human Dt/S protein contains a Lys functional site (amino acid 400-410) and a highly variable region (amino acid 413-442:). The murine DUB (mDUB) subfamily members are also different from other UBPs in terms of functional criteria. Subfamily members are very early genes that can be induced by cytokines and thus may play a regulatory role in cell growth or proliferation. At the same time, the Dt/J? protein is unstable and is rapidly degraded by proteolysis, which is mediated by ubiquitin, shortly after induction. The mDUB report printed by the Intellectual Property Office of the Intellectual Property Office of the Ministry of Economic Affairs proves that specific cytokines such as IL_2 and positive _3 induce specific ubiquitinated enzymes. (10) Proteins may modify the ubiquitin-proteolytic pathway to mediate specificity. Cell growth or 15 differentiation messages. Their modification is regulated transiently. The protein, for example, is rapidly and transiently induced by IL_2. Interfering with enzymes with specific isopeptide inhibitors can block specific cytokine signaling. In mammals, defensins constitute the main family of antimicrobial peptides. Depending on the distribution of 20 cysteine and the bond of disulfide bonds, human defensins can be divided into two categories: alpha-anti-purpurin found in granulocytes and small intestinal epithelial cells, and by epithelial cells and white blood cells ( Including macrophages) did not show _ anti-purple. Some defensins are expressed in a constitutive manner in granulocytes and epithelial cells. As for other defensins, they are exposed to microbial pathogens or primary inflammatory fines-13- This paper scale applies to China National Standard (CNS) A4 specifications (21〇 X 297 public interest) 1312008 A7 ---21___ _...··_ V. Description of the invention (u) Cytokines such as IL-lyS, TNF-α and interferon are induced. The gene encoding the human defensin is clustered in the 1 Mb fragment on chromosome 8Ρ23. In the recent gene amplification study, since the defensin could not be detected even in many mammals (including Zhabe), cold-defense 5 was proposed. The hormone may be earlier than the alpha-defensin family in the flood season. Dairy cows have at least 13 sputum-defensins, but no sputum: - defensins. Cold_defensins contribute to the early host defense against several bacterial and fungal pathogens and are an important mechanism for the innate immune response. In addition to this antimicrobial activity, both the immature dendritic cells and memory T cells, as well as the chemical attractant activity on single cells, have recently been described, demonstrating that stone defensins can be promoted as well as Adaptive two immune responses. SUMMARY OF THE INVENTION The Ministry of Economic Affairs, Intellectual Property Office, Staff Consumer Cooperatives, Printed in the present invention relates to murine DUBs analogs, which are found on chromosomes 4 and 8 and have hematopoietic specificity, which can be induced by cytokines. Protease, and its various regulatory regions. Using the murine DUb_1 and DUB-2 sequences, by searching the human genome database, eleven novel human DUBs previously described in the public database and four potential genes exhibiting truncated DUBs were identified. When gaps caused by deletions and N-terminal and/or 延伸-end extensions are not counted as mismatches, their genes share open reading frames (〇RFs) that are 20 88 to 99% amino acid identity. And exhibit approximately 50% identity to murine DUBs. Eight of the eleven RFs produced a protein of 530 amino acids. Two 〇RFs (hDUB83 and hDUB8.ll) have an internal in-frame (in_frame) deletion, allowing these genes to produce long-chain polypeptides with 497 and 417 amino acids, respectively. There is a Rf

A7 B7 1312008 V. INSTRUCTION DESCRIPTION (13) (hDUB4.5) has an extension on both the 5' and 3' sides, allowing the gene to be expressed as a long chain polypeptide having 574 amino acids. Surprisingly, this extension of the 5 produces a specific pr〇-polypeptide sequence that directs the polypeptide to the mitochondria. Furthermore, the various regulatory regions of their genes, which promote the identity of each other, also share nearly 90% identity, suggesting the coordination of their performance. In addition, we found that two of these genes can be expressed under the control of different promoters, each independently controlling and displaying potentially different protein products. Manipulating these gene products with small molecule compounds can (1) reduce inflammation by regulating the regulation of inflammatory cytokines; (2) regulate autoimmune diseases that are critical for lymphocyte proliferation by regulating cytokine receptor signaling. And (3) use the above mechanism to avoid overreaction during infection. The two gene clusters (hDUB4.1 and hDUB4.2) have two specific promoter functions before their ORFs, allowing them to independently regulate their potential for transcription. The longer transcripts of these ORFs (referred to as hDUB4.1a and liDIJB4.2a) have 12 and 14 expression sequences, respectively, and can produce long-chain polypeptides with 1016 and 1-21 amino acids, respectively. These peptides have a shorter C-terminal amino acid of the C-terminal printed form of the Intellectual Property Office of the Intellectual Property Office, which can be expressed separately from independent promoters (called liDUB4.1b and hDUB4_2b, respectively). In addition, there are two other 〇RFs that produce polypeptides longer than 530 amino acids (hDUB4.10 and hDUB4.ll). Obviously, the second derivative polypeptide shares significant homogeneity at the N-terminal portion (the inventor joins the sequence after the sequence file). Three of the ORFs (hDUB 4.5, 4.8 and 8.2) have N-terminal inserts, which are typical mitochondrial targeting sequences. The rows of the sequences are provided in the table below. -15- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention (Η) ratio. In addition to hDUB4.1a, promoter sequences defined as upstream of the ORF initiation ATG exhibit a significant degree of homogeneity with each other. In the 2000 base pair span of the starting ATG, except for: hDUB4.1a, the sequence identity between all promoter sequences is about 90%. The two promoter sequences 5_ (hDUB8.3 and hDUB8.11) have 334 nucleotides to enter at approximately 1 验 upstream of the starting ATG. Interestingly, hDUB8.3 and hDUB8.ll are the only ones with shorter 〇rfs due to internal deletions. In addition to their ORFs, there are 5 ORFs that can express peptides (hDUB4.4, hDUB4.9, hDUB8.2, hDUB8.9, and 10 hDUB8.10) 'Shared with other 530 amino acid long-chain peptides The Ministry of Economic Affairs, the Intellectual Property Office, and the Consumer Cooperative, printed codons, but terminated prematurely due to the termination sequence in the box. They also share a significant homogeneity upstream of the ATG start codon, suggesting that it can be expressed as a truncated protein with potential regulatory functions. All 11 hDUB8 genes are clustered together with the defensin group in the 2 Mb region of 8P23, suggesting that 15 acquisition and amplification is a fairly recent event 'may occur during the mammalian evolution. It is interesting to note that the 'hDUB4 gene group is also in the highly amplified cluster region of chromosome 4P16, and its position on the chromosome has not been specified. These data suggest that all hDUB4s and hDUB8s are located in the extremely dynamic region of human chromosomes (both 4p I6 and 8p23) undergoing volatile 20 amplifications. The data also suggests that the performance of hDUB8 is also coordinated to combine the defensins that are essential components of the immune response and inflammation. Search method for identifying mDUBs human analogues: In order to identify mDUBl, -2, -2A human analogues, use the -16-Nen-new scale to apply the Central Standards (CNS) A4 specification (210 X 297 public) ' ' 1312008 V. Description of invention (15)

Ensembl area comparison search engine (http://www.ensembl.org/perl/b1astview), with mDUBl (U41636), mDUB2 (NM-010089), and mDUB2A (AF3 9363 7) DNA sequences against Ensembl's entire "Golden Pathway" [Search in 5 contigs]. All three mDUBs have a significant alignment with AC083981 'AF252831, AF228730, AF252830, AC068974 on chromosome 8, including high scores above 2000 and a probability less than e-87. In order to find all the homologous genes in the genome, a detailed search was performed using the genomic alignment sequence to control the "Golden Pathway" contigs. Find out more of the two overlapping groups with a significant ratio, which is less than e-100: - AC074340 on chromosome 8, and AC022770 on chromosome 4. The DNA sequences of the contigs AC083981, AF252831, AF228730, AF252830, AC068974, AC074340, and 15 AC022770 were downloaded from Ensembl, and the GenScan gene annotation program was used to print the genetic annotations of each cascading group. The genes homologous to mDUBs are named sequentially according to their positions on the chromosome. For example, hDUB8.1 is derived from AF228730, 8.2, 8.3 is derived from AF252830, 8.5 is derived from AC074340, 8.6 is derived from AF252831, 8.7, 8.8 and 8.9 20 are derived from AC083981, and 8.10 and 8.11 are derived from AC068974. hDUB4.1, 4.2, 4.3, 4.4, 4.5 are derived from AC022770 on chromosome 4. Ensembl and NCBI area search were performed using their hDUB4s and hDUB8s'. Identification of further overlaps covering the chromosome 4 -17- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 1312008 V. Invention Description (16) Group NT_028165. This and the combined chromosome 4pl6.1 region were further annotated using the GenScan gene annotation program. Thus liDUB 4.6, 4.7, 4.8, 4.9, 4.1 〇, and 411 were identified. Analysis of the hDUB gene group in chromosome 4 revealed that nucleotide homology searches were performed with murine DUB 15 and 2 ' at least five ORFs were identified in the unmaPPed stack (AC022770). At least four of the five ORFs share a core 530 amino acid sequence. Two ORFs (hDUB4.1 and hDXJB4.2) are multiplexed expression sequences 〇RFs that extend the N-terminal portion of the polypeptide sharing the smallest sequence identity. However, a conserved putative promoter sequence containing more than 2,000 bases in the region of the insert sequence closest to the last expression sequence is conserved for all five genes. Three ORFs (hDUB 4.5, 4.8, and 8.2) have N-terminal inserts, which are typical mitochondrial targeting sequences. These hDUB genes are clustered on human chromosome 4P16, which is the portion of the human chromosome that is not mapped. 15 Analysis of hDUB gene group in chromosome 8 showed that the homology search of nucleotides was performed in two different contigs (AC068974, AC074340, AC083981, AF228730) with the mouse-based DUB1 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative. At least eleven 〇RFs were identified in AF252830, and AF252831). At least seven of the eleven ORFs share significant identity of the same length. All of the 20 genes have a conservative putative promoter sequence of more than 2000 testers. These hDUB genes are clustered on human chromosome 8ρ23·1 and clustered together with defensin molecules (at least 9 defensins clustered with hDUB8s), and the entire functional site belongs to the olfactory GPCR population. The analysis of the amino acid sequence of hDUBs showed that the peptide and mDUBs were -18-. The paper scale was applicable to the Chinese National Standard (CNS) A4 specification (210 X 297 public). 1312008 ---___________ — V. Description of invention (η) Highly conserved Cys and His functional sites containing active sites that may form enzymes. The putative active site nucleophilic substance of πι/Χ/β-2 is a cysteine residue of the cys functional site (Cys-^cmDUB- and m/X/D are rich in lysine-rich regions (Lys function) The site; mdi/5-2 amino acid 5 374-384) and the short high-variation region amino acid 385-451), wherein mDUB-1 and mDC/5-2 sequences are quite different. The high variation (HV) region contains a quinic acid sequence: a copy of pqEqNHqk. TaqMan simultaneous PCR analysis of hDUB4s and hDUB8s in human immune cells under various stimuli 10 Experimental procedure using random hexamer to total cellular RNA reverse transcription (RT) (using TaqMan reverse transcription reagent Cat#N808-0234) Ministry of Intellectual Property, Staff Consumer Cooperative, printed on 100 μl of lxTaqMan RT buffer mix, 5.5 mM MgC.h, 〇_5 mM dNTPs, 2.5 uM random hexamer, 40 IJ RNAase inhibitor, 125 U Multiscribe reverse transcriptase One microgram of 15 full RNA preparation was pipetted up and down to mix. At 25. (: 10 minutes incubation (hybridization step), 48 minutes at 48 ° C (reverse transcription), 95 ° C for 5 minutes (heat killing enzyme). The sample can be left in the machine (4 ° C), or stored in - 20 ° C. The amount of cDNA synthesis was determined by incorporating a small amount of radioactive dATP (or dCTP). The average efficiency of this experimental procedure was between 60 and 80% of the 20 RNA was converted to cDNA.

The experimental procedure for TaqMan simultaneous quantitative PCR contained 12.5 μl of all reactions except primers and probes. -19- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 I ________B7 , invention description () 1 χ main mixture (Applied Miosystems Cat #4304437), 0.9 uM pre-primer, 0.9 uM reverse primer, 1 μl of TaqManlRT product in 0.2 uM probe, pipet up and down to mix . A sample containing the GADPH primer pair and the probe was also prepared as a control group. Thermal cycling and simultaneous amplification were detected using the ABI PRISM 7900HT Sequence Detection System. The amount of the gene relative to the GADPH control group was obtained from the Ct value determined by the PCr exponential phase. The scorpion-probe group used and its specificity: primer 4.1 is only used for hDUB4.1 bow I sub-4.2 for hDUB4.2, 4.3, 4.5 and 8.1 bow I sub-8.3 for hDUB8.3 and 8.11 bow I 8.5 for hDUB8.5 only for 8.6 for hDUB8.6, 8.7 and 8, 8 15 Table h · hDUBs in PBMC with LPS (100 ng/ml) and PHA (5 μg/ml) for 7 hours Ministry of Performance Economy, Intellectual Property Bureau, Staff Consumer Cooperative, Printed Donor 1 After the stimulus, adjust the multiplier and then adjust the relative performance

-20- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 public) A7 B7 1312008 V. Invention description (19) Table 2. Stimulus 1.5, 7 with LPS (100 Ng/ml) The performance of hDUBs in 24 hours of PBMC (donor 3) 1.5 hours, 7 hours, 24 hours, 24 hours, stimulation, up regulation, relative expression, stimulation, up regulation, relative performance, stimulation, up regulation, relative performance, 4.2 2,4 64 336 35-2 1.8 12.5 8.3 0.4 I3.I 1 1.7 1 8.5 1.6 11 65.9 4.8 1.5 1-8 Table 3. Stimulation with LPS (100 Ng/ml) and/or PHA (5 μg/ml) 5 1.5, 7, 24 hours The performance of hDUBs in PBMC (donor 4) 1.5 hours, 7 hours, 24 hours, 24 hours, stimulation, up regulation, relative expression, stimulation, upward adjustment, multiple, relative performance, post-stimulation, life-adjustment, multiples, relative performance, LPS 42 0.4 39 2.5 54 1-6 48 8.3 0.5 5 1.6 6 1.1 7 83 0,9 1 1.5 1.7 1.4 2 8.6 0.6 26 1.7 57 1.0 21 PHA 4.2 3.5 367 4.4 94 0.9 26 83 L5 13 1.7 6 0.7 5 8*5 1.9 2 0.9 1 0.7 1 8.6 2.3 103 2,5 23 0.8 17 LPS + PHA 4.2 1.2 129 3.4 73 0.8 23 8.3 1.0 9 2.2 8 0.7 5 8.5 1.0 1 0.9 1.3 0.9 1.2 8.6 1.3 56 2.5 33 0.8 18 T-lymphocytes (donor 5) were printed with anti-CD4/CD28 and anti-CD40/IL-4, respectively, by the Intellectual Property Office of the Ministry of Economic Affairs. The amount of expression of B lymphocytes (donor 6) did not increase. -21- This paper scale applies to Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Inventive Note (20) Table 4. Using TaqMan analysis, using the introduction of 4.2 in different human organ groups hDUB4.2, 4.3, 4.5 and 8.1 performance organization type mean β2 浞α performance average Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed line gland muscle upper medullary brain hepatic gland glandular collateral kidney bone and brain Fetal heart, kidney, lung, breast, pancreas, pre-pregnancy, small intestine, spinal nerve, spleen, sputum, thymus, thyroid, tracheal, uterus, PBMC, control, PBMC, PMA, PBMC, PHA, PBMC, HMB, A549, THP-1, ovary (+ve), control group, 29.72 20.00 10.08 0.92 34.02 20.49 13.89 0.07 26.92 22.73 4.54 42.84 32.03 19.97 12.42 0.18 27.59 24.23 3.71 76.15 33.22 22.58 10.99 0.49 33.09 21.60 11.85 0.27 29.93 21.97 8.32 3.13 32.10 19.31 13.15 0.11 30.00 21.74 8.61 2.56 34.83 24.07 11.11 0.45 36.60 23.77 13.19 0.00 29.14 20.93 8.55 2.66 32.11 21.39 11.07 0.46 28.27 20.44 8.18 3.45 34.33 21.00 13.69 0.08 27.04 21.91 5.47 22.48 32.45 19.02 13.78 0.07 32 .15 21.66 10.84 0.55 28.57 23.07 5.87 17.16 31.01 20.68 10.69 0.61 28.84 20.80 8.39 2.97 31.39 19.63 12.11 0.23 30.37 21.09 9.64 1.25 33.98 18.82 15.52 0.02 33.62 18.81 15.17 0.03 34.20 18.77 15.78 0.02 34.23 17.81 16.77 0.01 31.98 21.57 10.77 0.57 35.48 20.75 15.09 0.00 31.84 21.55 10.65 0.62 29.61 21.86 8.11 3.62 -22- This paper scale applies to Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention (21) Table 5. Introduction to the Human Immune Cell Group 4.2 Measured hDUB4.2, 4.3, 4.5 and 8.1 performance average 89747613 13J16296 422814443 33 2 33333 B2 average ddCt performance 17.22 17.50 0.005 17.16 15.76 0.018 19.92 9.32 1.565 20.67 11.00 0.488 20.43 14.78 0.036 20.41 14.38 0.047 20.15 15.31 0.025 18.29 15.89 0.017 18.03 17.06 0.007 17.27 17.88 0.004 17.38 18.03 0.004 19.73 16.67 0.000 18.18 17.84 0.000 17.92 20.07 0.000 17.63 14.02 0.060 18.23 13.60 0.081 18.27 16.15 0.014 19.31 13.38 0.094 18.07 16.40 0.012 18.78 15.02 0.030 2 1.64 14.22 0.000 21.52 15.45 0.000 21.41 15.07 0.000 34.55 34.62 34.87 35.87 35.48 37.46 31.11 31.29 33.88 32.15 33.94 33.27 35.33 36.44 35.94 Cell type and stimulating conditions Granulocyte quiescent granulocyte TNF-ct 4/24 h 匸 019 (tonella - €0401〇€019(for peach-1^>5) FLS-REST FLS-IL1 4/24 hour FLS-TNF-a4/24 hour single cell quiescent state (collected at 1.5, 7, 24 hours) Single cell LPS (collected at 1.5, 7, 24 hours) Single cells 11^^ (collected at 1.5, 7, 24 hours) Single cell LPS & IFN-γ (collected at 1.5, 7, 24 hours) DCs blast cells (CD14+)

DCs immature DCs mature THO quiescent state THO activated Thl quiescent state Th1 CD28/CD3 Th2 quiescent state Th2 CD28/CD3 BSMC BSMCIL-4 + TNF-a 24 hours BSMC IL-13 + TNF-a 24 hours Ministry of Economic Affairs Intellectual Property Bureau Employee Consumption Cooperative Printed -23- This paper scale applies China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention Description (22) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 24- BSMC IL-4 + IL-I3 36.28 22.09 14.73 0.000 NHBEdO 36.63 22.24 14.92 0.000 NHBE IL-4 +TNF-a dO 35.72 21.42 14.83 0.000 NHBE IL-13 +TNF-adO 36.35. 21.37 15.52 0.000 NHBE quiescent state d7 + dl4 34.89 22.41 13.01 0.121 NHBE IL-4 + TNF-a d7 + dl4 38.59 22.02 17.11 0.000 NHBE IL-13 + TNF-a d7 + dl4 37.62 21.93 16.23 0.000 CD8T cells O hour 30.15 19.52 11.16 0.437 CD8 T cells a-CD3/CD28 4 Hours 32.08 19.6 13.01 0.121 CD8 T cells a-CD3/CD28 24 hours 30.94 18.64 12.84 0.137 HMVEC quiescent 35.09 20.25 15.38 0.000 HMVECTNF-a + IL-4 24 hours 35.91 20.86 15.59 0.000 HMVEC TNF-a 24 hours HMVEC TN Fa + CL-13. 24 hours 35.57 21.06 15.05 0.000 36.38 20.61 16.31 0.000 Normal joint synovial fluid collection 34.92 21.16 14.3 0.050 RA joint synovial fluid collection 33.65 20.88 13.3 0.099 Normal colon 32.5 21.68 11.36 0.381 Colitis colon 33.17 21.32 12.39 0.187 Collection Crohn's disease colon 32.91 22.06 11.39 0.374 normal lung 31.01 20.5 11.05 0.472 COPD lung 35.09 22.14 13.49 0.000 positive control group 28.4 22.29 6.64 9.992 This paper scale applies to China National Standard (CNS) A4 (210 X 297 mm) I3i2 〇〇8 ba V. Description of invention (23) Using PCR to transfer h DUB4, 8s The following primer set was used to transfer a single expression sequence hDUB4s from human genomic DNA and an amino acid of 530 amino acid in 8s: N Terminal primer. 5'-over ggaggacgactcactct-3' (19-mer) 5 C-terminal primer. 5'-ctggcacacaagcaga-3' (19-mer) In each primer, the three nucleotides of the underline represent the translation initiation and Stop the codon. This primer set amplifies most of hDUB4s and hDUB8s and possibly amplifies hDUBs that are sufficiently similar to hDUB4s and hDUB8s due to the high homogeneity of this partial ORF nucleotide sequence. 10 The 1593 base pair fragment was successfully amplified from the genome DNA from two healthy human subjects and propagated into the PCR2.1 vector and transformed into E. coli TOP10 strain. More than 300 independent transgenic plants with appropriately sized inserts were obtained and sequences were obtained using an ABI automated DNA sequencer. 15 Deubiquitination assays Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperatives Printed As previously described in the literature, the deubiquitination assay using the previously identified pancreatic hormone galactosidase fusion protein confirms that DUB is deubiquitinated. Hormone enzymes. Briefly, PCR is used to generate a DUB fragment containing about 1,500 20 nucleotides and a cDNA containing a mis-sense mutation based on wild-type cDNA (corresponding to 1 to about 500 amino acids), and the boxes are aligned. Embedding into pGEX (Pharmacia) downstream of the glutathione transferase (GST) coding element. Ub- using plastids based on pACYC184

Met--ga was co-transformed into E. coli MC1061 as indicated. -25- This paper size is applicable to China National Standard (CNS) A4 specification (210 X 297). A7 B7 1312008 V. Inventive Note (24) Dissolve plastid E. coli MC1061 cells, using rabbit anti-gal antiserum (Cappel), rabbit anti-GST antiserum (Santa Cruz), and ECL system (Amersham Corp.) were analyzed by immunoturbation. The purified ubiquitinated protein was used as a substrate, and the purified hDUB fusion egg 5 white was used to demonstrate in vitro ubiquitinated enzyme activity. UDVB4S and hDVB8s are potentially inflammatory cytokine-specific very early genes. The Ministry of Economic Affairs, the Intellectual Property Office, and the Consumers' Cooperatives, were originally transferred to the very early base 10 induced by IL_3. Similarly, Saiyuzhou-2 was transferred to a very early gene that can be induced by IL-2. We used a variety of TaqMan assays for human organ RNA samples and human immune cell RNA samples to detect the inducibility of their genes as well as cell type-specific expression. Our data suggest that the performance of hDUBs is not apparent in lymphocytes and granulocytes, but only from several donors. 15 Human PBMC t' has two manifestations. This result strongly suggests that performance may be limited to single-cell/maize cells and potentially cells. hDUB4s and hDUB8s are up-regulated in PBMC when stimulated by stimuli (Lps and/or pHA) known to upregulate a variety of inflammatory cytokines such as TNF-α, IL-1 stone. The increase in performance is as follows: after stimulation for 2 to 24 hours, almost complete loss of af is not an early gene. After 15 hours of stimulation, only weak performance is up-regulated. This fact is dark +, ώ g does not irritate itself may not regulate hDUB4s and hDUB8s, I ^ . However, the cytokine that regulates upwards after several hours of stimulation may be Qing B4s. The reason for the upward adjustment of the muscles. , -26- This paper scale applies Chinese National Standard (CNS) A4 A7 B7 1312008 V. Invention Description (25) The DUB subfamily of the ubp superfamily from their data, we propose that hDUB4s and hDUB8s are different from ubiquitinated enzymes. A member of the subfamily, which shows the greatest similarity to the mDUB subfamily (including 5 mDUBl, mDUB2, and mDUB2A), called the subfamily. Subfamily members contain different structural features that distinguish them from other ubps. First, the (10) subfamily members are slightly smaller enzymes containing about 500 to 550 amino acids; second, the subfamily members share the amino acid similarity', including not only the Cys and His functional parts, but also the entire main 10 Amino acid sequence. For example, the D05 protein contains an amino acid-rich region (Lys functional site) and an HV functional site near its carboxy terminus. TRANSFACFind, a dynamic programming method, is printed by the Intellectual Property Office of the Intellectual Property Office of the Ministry of Economic Affairs, see Heinemeyer, T., et al., “Expanding the TRANSFAC database towards an expert system of regulatory molecular 15 mechanisms” Nucleic Acids Res. 27, 318 -322, (1999), analysis of the main structure of each DUBs regulatory region (or promoter region) in combination with putative transcription factors. The Transfac database provides eukaryotic cis- and trans-acting regulatory elements. -27- This paper scale is applicable to China National Standard (CNS) A4 specification (210 X 297 mm). 1312008 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (26) Table 6. DUB regulation of hDUB4.1a ( The main structure of the putative transcription factor in the region or the activator region (position is expressed in nucleotides)

Transfac M00271 M00148

MQ024Q M00083mmi M00253 Position (score > 729..724( KX?) 296..302(100) 1016..1010(96) 958..964(94) 474..480(94) 1982..1988(92 ) 129..123(90) 857..863(90) 776..732(90) 1919.1913(90) 1227„1233(90) 276..282(90) 1741..1735(90) 193..199 (90) r〇S.. 111(90) 1600:.1606(100) 700,.694(100) 892.>899(100) 162.,156(100) 1008..1002(1001 423.. 429(100) 153,447^99) 359..353(98) 1388..1394(98) 1644..1650(97) 1702..1696(97) 250,,256(97) 231,.237(97) 617..611(94) 509..503(93) 432..426(92) 丨307313(92) 153..159(92) 1832>.1B38(92> 1366..1372(92) 494. &gt ;500(92) 1450..1456(91) 1456..1450(91) 722..716(90) 99L.9aS(90) 986..992(90) 1646^1640(90) 1142,.1149( 97) 1344..1351(96) 639..632(95) 1313>.1320(94) 1872..1879(93) 269..262(92) 257..250(91) 1103·· 1110(91 ) 745..752(91) 名糗AML-la

SRY ι2·5 MZF1

CdxA cap -28 Description ·!, livestock (runt)-factor AML~1 Sex-determined region Y gene product Same as #功统位因子Nkx-2.5/Csx, iron iron K(tinman) a mass MZF1

CdxA transcription start cap message

This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm). 1312008 A7 B7 V. Invention Description (27) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1589..1596(90) M00099 978.. 993(96) S8 S8 1637..1652(94) 995..980(93) MOOIOO 162..156(96) CdxA . CdxA 1008..1002(96) 423-429(96) 1774..1768(96 ) 415..421(92) 860-854(91) 1026..1020(91) 494..500(91) 94..100(91) M00285 725..713(95) TCF11 TCFl l/KCR-Fl /Nrfl Homogeneous Dimer 982..970(92) M00347 531..522(95) GATA-1 GATA-binding factor i MOOL35 1642..1660(95) Oct-1 Octamer Factor 1 M00075 217..226 (94) GATA-L GATA-binding factor 1 M00278 530..522(94) Lrao2 binds to Tal-1, E2A protein, and GATA-1 complex of Lmo2, half-site 2 900..908(90) M00157 990..978(94) RORa2 RAR-related orphan by M00I27 533..520(93) GATA-1 GATA-binding factor 1 M00109 1933..1920(93) ΟΕΒΡβ CCAAT/promoter binding protein call M00190 1656. .1643(93) C/EBP CCAAT/promoter binding factor M00137 1193.1205(93) Oct-1 octamer factor 1 248..260(90) 1652..1640(90) M00302 1501..1512(92) NF-AT nuclear factor M00077 for activated T cells 900.908(91) GATA-3 GATA-binding factor 3 530., 522(90) M00126 533..520( 91) GATA-1 GATA-binding factor 1 M00159 1231..1219(91) C/EBP CCAAT/promoter binding protein M00074 1280..1292(91) c-Ets-l(p54) c-Ets-l(p54 M00042 192..201(91) Sox-5 Sox-5 M00241 1650..1643(91) Nkx-2.5 Synergy functional part factor Nkx_2.5/C!ac, blacksmith homogenizer M00116 138..125(91 C/EBPa CCAAT/promoter binding protein a M00138 1640..1662(91) Oct-1 octamer factor i M00128 532..520(90) GATA-l GATA-binding factor 1 M00248 1645..1656(90 Oct-1 octamer factor 1 _ M00289 1009..1021(90) HFH-3 HNF-3/Fkh homology Table 7_ hDUB 4. The binding of the putative transcription factor in the DUB regulatory (or promoter) region of lb Structure (position is expressed in nucleotides) π Transfac Position (score) Name Description.... M00254 1831..1820(99) CCAAT Cellular and viral CCAAT ft: -29- This paper size applies to China Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention 28) Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed M00101 832..826(98) CdxA CdxA 727..721(92) 70..64(92) 570..564(92) 523..529(92) 425..431(92) 1682..1688(91) 1409..1415(91) 1415..1409(91) 1688..1682(91) M00054 470..461(97) NF-/CB NF-^ B 643..634(95) M00148 946..940(96) SRY Sex-determining region Y gene product 1564..1570(92) 1528..1534(92) 1092..1098(92) 1048..1054(90 ) 708..714(90) 655..661(90) 1360..1354(90) 1824..1818(90) 396.390(90) 749..743(90) 1016..1010(90) 302.. 308(90) M00053 470..461(95) c-Rel c-Rel 643..634(94) M00285 1734..1746(95) TCFII TCFl l/KCR-Fl/Nrf 1 Homogeneous Dimer 1111.. 1123(91) 17..5(90) M00052 470..461(95) NF-/CB NF-/cB(p65) 643..634(94) M00077 1933..1941(95) GATA-3 GATA- Binding factor 3 M00253 485..492(95) cap transcription initiation cap message 1893..1886(95) 749..756(94) 834..841(93) 1484..1477(92) 511..504 (92) 1194..1201(91) 163..170(91) 321.328(91) 340..347(91) 1815..1808(90) 563..570(90) M00096 652..660(95) Pbx-l Pbx-l M 00194 472..459(95) NF-/CB NF-zcB M00209 1818-1831(94) NF-Y NF-Y binding site -30- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 A7 B7 1312008 V. Inventive Note (29) M00116 1238..1225(94) C/EBPalpha CCAAT7 Promoter Binding Protein a M00203 1699..1689(94) GATA-X GATA Binding Site 1227..1217 ( 90) M00241 535..542(94) Nkx-2.5 homologous work site factor Nkx-2.5/Csx, foreign trader M00033 759..746(94) p300 p300 333..320(90) M00127 1703.1690( 93) GATA-l GATA-binding factor 1 M00I58 323-310 (93) COUP-TF COUP/HNF4 heterodimer M00075 1889..1898(93) GATA-l GATA·binding factor 1 142-133(90) 1736 ..1745(90) M00286 963..976(93) GKLF ^ wins the increment of Krueppel's factor M00278 1933..1941 (93) Lmo2 binds to Tal-1, E2A protein, and GATA-1's Lmo2 Complex, half site 2 M00076 1932..1941 (93) GATA-2 GATA-binding factor 2 983..992(92) M00208 471..460(93) NF-/CB NF-/cB binding site M00185 1829..1819(92) NF-Y nuclear factor Y (Y-匣 binding factor} M00302 232.243(92) NF-AT activation Nuclear factor M00348 98..107(92) GATA-2 GATA-binding factor 2 M00134 308..326(92) HNF-4 Hepatic nuclear factor 4 M00223 548..540(92) STATx message transducer and Transcriptional activation 刽M00039 1046..1039(92) CREB cAMP-inductive element binding|White M00271 1955..1960(92) AML-la Small livestock-factor AML-1 M00074 231..243(91) c-Ets-l (p54) c-Ets-l(p54) 252..264(91) M00289 385..397(91) HFH-3 HNF-3/Fkh homogenate 3 (= Freac-6) M00199 1722..1714(91 AP-1 ΑΡ·1 binding site M00032 254..263(91) c-Ets-l(p54) c-Ets-l(p54) M00147 782..773(91) HSF2 heat shock factor 2 M00100 1101. .1095(91) CdxA CdxA M00042 650..659(90) Sox-5 Sox-5 M00183 1026..1035(90) c-Myb c-Myb M00240 963..957(90) Nkx-2.5 homologous functional part Factor Nkx-2.5/Gsx, blacksmith homogenizer 1272..1266(90) M00190 1238..1225(90) C/EBP —CCAATV promoter combined with stem " M00083 49..42(90) MZFl MZF1 M00184 275 ..284(90) MyoD myocyte determinant M00087 980..991(90) Ik-2 Ikaros 2 M00221 1860..1850(90) SREBP-l sterol regulatory element-binding protein 1 - M00137 13 88..1376(90) Oct-1 Octa-Factor Factor 1 Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed Table 8. The main broadcast of the combined transcription factor in the DUB regulatory (or promoter) region of hDUB 4.2a ( The position is expressed in nucleotides. ' ' α -31- This paper scale applies to China National Standard (CNS) A4 specification (2W X 297 mm) A7 B7 1312008 V. Invention description (30) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative system

Transfac position (score > name description MOO 148 992..986(100) SRY sex determination area Y gene 物 942..948(100) 919..913(96) 1544..1550(92) 1505..1511 (92) 815..809(90) 1068..1074(90) 1196..1190(90) 1337..1331(90) 680..686(90) 1697..1691(90) 1802..1796( 90) 368..362(90) 721..715(90) 274..280(90) MOOIOO 1077..1071(10 〇) CdxA CdxA M00271 1933..1938(10 〇) AML-la Small livestock-factor AML-l 2204., 2209(92) M00101 1077..1071(99) CdxA CdxA 805..799(98) 699..693(92) 1384., 1390(92) 936..942(92) 495. .501(92) 1660..1666(91) 1666..1660(91) M00076 716..707(98) GATA-2 GATA-binding factor 2 1910..1919(95) 959..968(92) 1679 ..1670(91) M00285 1712..1724(96) TCF11 TCFl l/KCR-Fl/Nrf 1 Homogeneous Dimer 1087..1099(91) M00272 1242..1251(96) p53 Tumor suppressor p53 M00253 135 ..142(96) cap transcription start message 457..464(95) 1871..1864(95) 721..728(94) 1461..1454(92) 312..319(92) 1989. .1996(92) 1855..1848(91) 770..777(90) 1793..1786(90) 295..302(90) -32- This paper ruler Applicable to China National Standard (CNS) A4 Specification (210 X 297 mm) 1312008 V. Invention Description (31) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1274..1281(90) M00106 627..636(95) CDP-like cut-like homologous functional site protein 634..625(93) ΜΟΟΠ6 1215..1202(95) C/EBPa (: 匚八八17 Promoter binding protein "M00254 1809..1798( 95) CCAAT cellular and viral CCA AT匣M00249 1117..1105(95) CHOP-C/ΕΒΡα CHOP and C/EB Ρα heterodimer M00054 442.,433(95) NF-zcB NF-/CB M00147 2182..2173(94) HSF2 Heat shock factor 2 2173..2182(92) 754..745(91) 141..132(90) M00104 634..625(94) CDP-like homologous function Partial protein 2..11(92) M00I34 280..298(94) HNF-4 Hepatic nuclear factor 4 M00052 442..433(94) NF-zcB NF-/cB(p65) M00053 442..433(94) c-Rel c-Rel 785..794(90) M00033 731..718(94) p300 p300 M00158 295..2'82(93) COUP-TF COUP/HNF4 Heterogeneous Dimer M00032 225..216(93 c-Ets-l(p54) c-Ets-I(p54) 226..235(93) M00172 1851..1861(92) AP-l Activator Protein 1 M00223 520.512(92) STATx message transducer and transcriptional activator M00075 1679..1670(92) GATA-1 GATA-binding factor 1 1867..1876(91) 716..707(91) 316.307(90) M00184 1463.1472(91) MyoD muscle Embryonic cell determinant 1472..1463(91) 247..256(90) 2057..2048(90) M00289 357.369(91) HFH-3 HNF-3/Fkh homogenate 3 (= Freac-6) M00109 1202. .1215(91) C/EBP/S CCAAT/promoter binding protein/5 2036..2023(90) M00268 937..950(91) XFD-2 Xenopus and head functional part factor 2 M00208 443..432 (90) NF-/CB NF-«B binding site M00173 1851..1861 (90) AP-l activator protein 1 M00183 1002..1011(90) c-Myb c-Myb 2020-2011(90) M00240 217-211(90) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, foreign blacksmith homogenate M00188 1851..1861 (90) AP-l activator protein 1 M00099 1086..1101(90) S8 S8 M00302 813..802(90) NF-AT nuclear factor M00083 for activation of T cells 21..14(90) MZF1 MZF1 M00190 1215..1202(90) C/EBP CCAAT/promoter binding factor J M00221 1838..1828( 90) SREBP-1 sterol regulatory element-binding protein 1 -33- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. INSTRUCTIONS (32) M00294 949..937(90) HFH-8 HNF-3/Fkh Homogeneous-8-~~-__ MOO 137 1365..1353(90) Oct-1 Eight Elements Factor 1 -------- M00077 1911..1919(90) GATA-3 GATA-binding factor 3 -----__ M00194 444..431(90) NF-/CB NF-/CB ----- Table 9. The main lexicon of the putative transcription factor in the DUB regulation (or promoter) region of hDUB 4.2b (position is expressed in nucleotides) ''σ Ministry of Economic Affairs Intellectual Property Bureau employee consumption Cooperative printing

Transfac position (fraction) Name Description MOO 100 1102..1096(100) CdxA CdxA MOO 148 1017..1011(100) SRY Sex determination region Y gene product 967..973(100) 944..938(96) 1566. .1572(92) 1530..1536(92) 840..834(90) 1093..1099(90) 705..711(90) 1362..1356(90) 1719..1713(90) 1824.. 1818(90) 393..387(90) 746..740(90) 299..305(90) M00253 1120..1127(99) cap transcription start cap message 160..167(96) 482.. 489(95) 1893..1886(95) 746..753(94) I486.· 1479(92) 337..344(92) 1877..1870(91) 795..802(90) 1815..1808 (90) 320..327(90) 1299..1306(90) MOOiOl 1102..1096(99) CdxA CdxA 830..824(98) 1231..1225(98) 1409..1415(92) 724. .718(92) 520..526(92) 1682..1688(91) 1688..1682(91) M00076 741..732(98) GATA-2 GATA-binding factor 2 r 1932..1941(95) 984..993(92) 1701..1692(91) -34- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention Description (33) Ministry of Economics Intellectual Property Bureau employee consumption cooperative printed M00285 1734..1746(96) TCF11 TCTU/KCR-Fl/N Rfl Homogeneous dimer 1112..1124(91) M00272 1267..1276(96) p53 Tumor suppressor d53 M00106 652..661(95) CDP-like homologous functional site protein 659..650(93) M001I6 1240..1227(95) C/ΕΒΡα CCAAT/promoter binding protein α M00254 1831..1820(95) CCAAT &cytogenetic and viral CCAAT匣M00249 1142..1130(95) CHOP- C/EBPa CHOP舆Heterogeneous dimer of C/ΕΒΡα M00054 467..458(95) NF-KB NF-/CB M00104 659..650(94) CDP 'The homologous functional part of the cut is white.27..36(92) M00134 305..323(94) HNF-4 Hepatic nuclear factor 4 - M00052 467..458(94) NF-zcB NF-«B(p65) M00053 467..458(94) c-Rcl c-Rel M00033 756 ..743(94) p300 p300 ' M00158 320..307(93) COUP-TF fc〇UP/HNF4 heterodimer M00075 1889..1898(93) GATA-1 GATA-binding factor 1 1701..1692( 92) 741..732(91) 341..332(90) 64L.632(90) M00160 965-976(93) SRY sex determination region Y gene product M00032 250..241(93) c-Ets- Kp54) c-Ets-l(p54) 251..260(93) M00172 1873..1883(92) AP-1 Activator Protein 1 M00223 545..537(92) STATx Message Transducer and Transcription Chemical agent M00271 1955..1960(92) AML-la Small livestock-factor AML-1 M00184 1488..1497(91) MyoD Muscle cell determinant 1497..1488(91) 272..281(90) M00289 382..394(91) HFH-3 HNF-3/Fkh homogenate 3 i= FreacO M00109 1227..1240(91) C/EBPjS CCAAT/promoter binding 1 white β M00147 779..770(91) HSF2 heat Shock factor 2 166..157(90) M00208 468..457(90) NF-zcB NF-zcB binding site M00183 1027..1036(90) c-Myb c-Myb M00173 1873..1883(90) AP -1 Activator protein 1 M00240 242..236(90) Nkx-2.5 Homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate M00188 1873..1883(90) AP-1 Activator protein 1 *= M00302 838..827(90) NF-AT nuclear factor M00083 activated T cells 46..39(90) MZF1 MZF1 " M00190 1240..1227(90) C/EBP CCAAT/promoting factor binding early-35-ben Paper scale applies to China National Standard (CNS) A4 specification (210x297 mm) A7 B7 1312008 ι, invention description (34 M00096 1115..1123(90) Pbx-1 Pbx-1 —~ M0Q221 1860..1850(90) SREBP -l sterol regulatory element - knot M00194 469..456(90) NF-ajB NF-λΒ '-' M00077 1933..1941(90) GA TA-3 GATA-binding factor 3~~~-

Table 10. Master of combined putative transcription factors in the DUB regulatory (or promoter) region of hDUB 4.3 (location in nucleotides) Ministry of Economic Affairs Intellectual Property Bureau Bayer Consumer Cooperative Print

Transfac position (score > name ~~~~----- M00148 1015.. 1009(100) 965.. 971(100) 942.. 936(96) 1566.. 1572(92) 1528-1534(92 ) 838.. 832(90) 1091.. 1097(90) 1219.. 1213(90) 703.. 709(90) 1360.. 1354(90) 1719.. 1713(90) 1824.. 1818(90) 391.. 385(90) 744.. 738(90) 297.. 303(90) SRY Sex-determining region Y gene product-;--- M00100 1100..1094(100) CdxA CdxA MOOlOt 1100.. 1094(99 ) 828.. 822(98) 1407.. 1413(92) 722.. 716(92) 959.. 965(92) 518.. 524(92) 1682.. 1688(91) 1688.. 1682(91) CdxA CdxA M00076 739.. 730(98) 1932-1941(95) 982.. 991(92) 1701.. 1692(91) GATA-2 GATA·binding factor 2 M00285 1734..1746(96) TCF11 TCF11/KCR -Fl/Nrf 1 Homogeneous Polymer 1110..1122(91) M00272 1265..1274(96) p53 Limb suppressor p53 ------ M00253 158.. 165(96) 480„487(95) 1893.. 1886(95) 744.. 751(94) 1484.. 1477(92) 335.. 342(92) 1877.. 1870(91) 793.800(90) cap transcribes the cap message of 4 • 36- tj The standard of the paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention Description (3 5) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed 318..325(90) - 1297..1304(90) M00106 650..659(95) CDP similarly cut homologous palladium site protein 657..648 (93) M00116 1238..1225(95) C/EBPcr tCAAT/promoter binding protein α - m M00254 1831..1820(95) CCAAT Cellular and disease Qin CCAAT匣M00249 1140·· 1128(95) CHOP- Heterogeneous dimer of C/EBPa CHOP舆C/ΕΒΡβ~ M00054 465..456(95) NF-ΛΒ NF-/CB M00104 657..648(94) CDP-like homologous functional site protein. 34(92) M00134 303..321(94) HNF-4 Hepatic nuclear factor 4 M00052 465..456(94) NF-/CB NF-/cB(p65) M00053 465..456(94) c-Rel c -Rel M00033 754..741(94) p300 p300 M00158 318..305(93) COUP-TF COUP/HNF4 Heterodimer M00075 1889,.1898(93) GATA-1 GATA-binding factor 1 1701..1692 (92) 739..730(91) 339-330(90) M00160 963..974(93) SRY sex determining region Y gene product M00032 248..239(93) c-Ets- l(p54) c-Ets -l(p54) 249..258(93) M00172 1873,,1883(92) AP-l Activator Protein 1 M00223 543..535(92) STATx Message Transducer and Transcription Activator M 00271 1955..1960(92) AML-la Small Livestock-Factor AML-1 M00173 1873..1883(91) AP-l Activator Protein 1 M00184 1486..1495(91) MyoD Muscle Embryo Determining Factor 1495.. 1486(91) 270..279(90) M00289 380..392(91) HFH-3 HNF-3/Fkh homogenate 3 (= Freac»6) M00109 1225..1238(91) C/EBP/S CCAAT /promoter binding protein point M00147 777..768(91) HSF2 heat shock factor 2 164..155(90) M00208 466..455(90) NF-/CB NF-rtB binding site M00183 1025..1034( 90) c-Myb c-Myb M00240 240..234(90) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate M00099 1109..1124(90) S8 S8 _ M00302 836..825 (90) Nuclear factor of NF-AT-activated T cells. M00083 44.37(90) MZF1 MZF1 _ M00190 1238..1225(90) C/EBP CCAAT/promoter binding factor - M0022I 1860..1850(90) SREBP-1 Solid Fermentation Regulatory Element - M-1 White 1 M00174 1873..1883(90) AP-l Activator Protein 1 - -37- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention Description (36 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed Table ii. DUB of hDUB 4.4 The main construct of the putative transcription factor in the control (or promoter) region (position is expressed in nucleotides) Transfac position (score > Name Description M00100 1101..1095(100) CdxA CdxA M00148 1016..1010(100) SRY Sex Determination Region Y Gene Product 966..972(100) 944..938(96) 1566..1572(92) 1529..1535(92) 840..834(90) 1092..1098(90) 705 ..711(90) 1361..1355(90) 1719..1713(90) 1824..1818(90) 393..387(90) 746..740(90) 299..305(90) M00101 1101 ..1095(99) CdxA CdxA 830..824(98) 1230" 1224(98) 1408..1414(92) 724..718(92) 520..526(92) 1682..1688(91) 1688 ..1682(91) M00076 741..732(98) GATA-2 GATA-binding factor 2 1932..1941(95) 983..992(92) 1701..1692(91) M00350 876..867 (% GATA-3 GATA-binding factor 3 _ M00285 1734..1746(96) TCF11 TOF11/KCR-Fl/Nrfl Homogeneous dimer 1111..1123(91) M00272 1266..1275(96) p53 Sleeping tumor suppressor P53 - M00253 160..167(96) cap transcription initiation cap message 482..489(95) 1893..1886(95) 746..753(94) 1485..1478(92) 337..344( 92) 1877..1870(91) 795..802(9 0) 1815..1808(90) 320.327(90) -38- M00077 1933..1941(90) GATA-3 GATA-binding "3----- M00194 467..454(90) NF-ΛΒ NF- ΛΒ —------ -------! ^The paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 V. Invention description (37) A7 B7 Ministry of Economic Affairs Intellectual Property Bureau Begong Consumer Cooperative Printed 1298..1305(90) M00106 652..661(95) CDP Similar cut-off functional site protein 659..650(93) M00116 1239..1226(95) ΟΕΒΡα CCAAT/promotion Subbinding protein α M00254 1831-1820(95) CCAAT Cellular and viral CCAAT匣M00249 1141..1129(95) CHOP-C/EBPa CHOP and C/ΕΒΡα heterodimer M00349 876..867(95) GATA-2 GATA-binding factor 2 M00054 467,.458(95) NF-zcB NF-ΛΒ M00104 659..650(94) CDP cleavable homologous functional site protein 27..36(92) M00134 305- 323(94) HNF-4 Hepatic nuclear factor 4 M00052 467..458(94) NF-acB NF-/cB(p65) M00053 467..458(94) c-Rel c-Rel M00033 756..743(94 ) p300 p300 M00348 876..867(93) GATA-2 GATA·binding factor 2 M00158 320-307(93) COUP-TF C0UP/HNR4 heterodimerization M00075 1889..1898(93) GATA-1 GATA-binding factor 1 1701..1692(92) 741..732(91) 341-332(90) M00160 964..975(93) SRY sex determining region Y gene Product M00347 876..867 (93) GATA-1 GAT in-binding factor 1 M00032 250..241(93) c-Ets-l(p54) c-Ets-l(p54) 251..260(93) M00172 1873..1883(92) AP-1 Activator Protein 1 M00223 545..537(92) STATx Message Transducer and Transcription Activator M0027I 1955..1960(92) AML-la Small Livestock-Factor AML-1 M00184 1487..1496(91) MyoD myocyte determinant 1496..1487(91) 272..281(90) M00289 382..394(91) HFH-3 HNF-3/Fkh homogenate 3 (= FreacA) M00109 1226..1239(91) C/EBPiS CCAAT/promoter binding protein/3 M00208 468..457(90) NF-ΛΒ NF-/CB binding site M00183 1026..1035(90) c-Myb c- Myb M00173 1873..1883(90) AP-l Activator Protein 1 M00240 242..236(90) Nkx-2.5 Homologous Functional Site Factor Nkx-2.5/Csx, Blacksmith Homogeneous M00188 1873..1883(90) AP-l activator protein 1 M00099 1110..1125(90) S8 S8 M00302 838..827(90) NF-AT nuclear factor M00083 for activated T cells 46..39(90) MZFl MZF1 M00 190 1239-1226(90) OEBP CCAAT/promoter binding factor M00147 166..157(90) HSF2 heat shock factor 2 M00080 874..864(90) Evi-I ectopic viral integration site 1 coding factor M000S2 874..864(90) Evi-i Ectopic Viral Integration Site 1 Codification Factor M00221 1860..1850(90) SREBP-l Solid Fermentation Control Element-Binding Protein 1 -39- • Set · Paper Size Applicable to the National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention (38) M00I94 469..456(90) NF-ΛΒ NF-/CB ----- M00077 1933. .I94K90) GATA-3 GATA-binding factor 3 --- Table 12. The main structure of the putative transcription factor in the DUB regulatory (or promoter) region of hDUB4.5 (position expressed in nucleotides) Ministry of Economic Affairs Intellectual Property Office Printed by employee consumption cooperatives

Transfac position (score) Name ~~~ --- MOO 148 963..969(100) SRY Sex determination region Y gene product 1013..1007(100) 940..934(96) 1526,.1532(92) 1089. .1095(90) 836..830(90) 701..707(90) 1358..1352(90) 1720..1714(90) 1825..1819(90) 389..383(90) 742.. 736(90) 295.301(90) M00100 1098..1092(100) CdxA CdxA ' ~ MOOlOl 1098..1092(99) CdxA CdxA 826..820(98) 1405..1411(92) 720..714(92 C57-2. 92) 1702..1693(91) 1299..1308(90) M00285 1735..1747(96) TCF11 TCh l/KtR-Fl/NrfI with f dimer 1108..1120(91) M00272 1263..1272 (96) p53 tt痼 inhibitor p53 M00253 156..163(96) cap transcription initiation cap message 478..485(95) 742-749(94) 1482..1475(92) 333..340(92 791.-798(90) 1816..1809(90) 316..323(90) M00106 648..657(95) CDP-like cut-off functional site protein 655..646(93) M00116 1236. .1223(95) C/EBPa CCAAT/promoter binding protein α _ M00254 1832..1821(95) CCAAT Cytotoxic and viral CCAAT匣 -40- This paper size applies to Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention (39)

M00249 1138..1126(95) CHOP·C/EBPa CHOP舆C/EBPcr heterodimer M00054 463..454(95) NF-ΛΒ NF-ΛΒ M00183 1493..1484(94) c-Myb c- Myb 1023..1032(90) M00104 655..646(94) CDP-like homologous functional site protein - 23..32(92) M00134 301.319(94) HNF-4 leaf nuclear factor 4 ---- - M00052 463..454(94) NF-/cB NF-«B(p65) M00053 463..454(94) c-Rel c-Rel 806.815(90) M00033 752..739(94) p300 p300 M00I58 316 ..303(93) COUP-IT COUP/HNF4 Heterogeneous Dimer M00032 246..237(93) c-Ets- Kp54) c-Ets-l(p54) 247..256(93) M00278 1300..1308 (92) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Lmo2 complex, half site 2 M00223 541..533 (92) STATx message transducer and transcriptional activator M00075 17021.1693(92) GATA -l GATA-binding factor 1 737..728(91) 337.328(90) M00077 1300..1308(91) GATA-3 GATA-binding factor 3 M00289 378..390(91) HFH-3 HNF-3/Fkh Homogeneous 3 (= Freac «6) M00109 1223..1236(91) ΟΕΒΡβ CCAAT/promoter binding protein/8 M00268 958-971(91) XFD-2 Claw and shape functional part factor 2 M00147 775..7 66(91) HSF2 heat shock factor 2 162..153(90) M00208 464..453(90) NF-/CB NF-κΒ binding site M0024Q 238..232(90) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homomorphism M00099 1107" 1122(90) S8 S8 M00302 834..823(90) NF-AT Nuclear factor M00083 for activated T cells 42..35(90) MZF1 MZFI M00184 268.. 277(90) MyoD Muscle Embryo#»Running determinant M00190 1236..1223(90) C/EBP CCAAT/promoter binding factor M00221 1861..1851(90) SREBP-i Infertility regulatory element-binding protein 1 M00294 970 ..958(90) HFH-8 HNF-3/Fkh Homo -8 ~ M00137 1386..1374(90) Oct-1 Octamer Factor 1 ~' M00194 465..452(90) NF-«B NF -/CB Ministry of Economic Affairs Intellectual Property Bureau Bayer Consumers Cooperative Printed the main structure of the putative transcription factor in the DUB regulatory (or promoter) region of hDUB 8.1 (position is expressed in nucleotides)

Transfac Position (Score) Name Li ~ M00148 1015..1009(100) SRY Sex Determination Area Y Gene Product 965..971 (1001 942..936(96) 1544..1550(92) -41- This paper size applies China National Standard (CNS) A4 Specification (210 X 297 mm) 1312008 A7 B7 V. Description of Invention (40) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 838-832(90) 702..708(90) 1719- 1713(90) 1824..1818(90) 390.384(90) 1384..1390(90) 1356..1350(90) 296..302(90) 63..69(90) MOOIOO 1100..1094(100 CdxA CdxA 724..730(96) 1547..1541(91) MQ0349 93..102(100) GATA-2 GATA-binding factor 2 M00350 93..102(100) GATA-3 GATA-binding factor 3 M00241 1800.*1807(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, foreign blacksmith homogenate M00348 93..102(100) GATA-2 GATA-binding factor 2 M00101 1100..1094(99) CdxA CdxA 828..822(98) 1229..1223(98) 1203..1209(94) 721..715(92) 959..965(92) 1386..1380(92) 65..59(92) 517..523(92) 419..425(92) 1682..1688(91) 1688..1682(91) M00203 95..105(98) GATA-X GATA binding site M00347 9 3..102(97) GATA-1 GATA-binding factor 1 M00075 1838..1847(97) GATA-1 GATA-binding factor 1 1701..1692(95) 137..128(92) 1483..1492( 92) 1889..1898(91) 1736..1745(90) M00158 317..304(96) COUP- TF COUP/HNF-4 Heterodimer M00253 158..165(96) cap Transcription initiation cap Message 1794..1801(95) 479..486(95) 1297..1304(93) 337..344(93) 48..55(93) 1484..1477(92) 1324..1317(91) 557..564(91) 1893..1886(91) -42- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm). 1312008 A7 B7 Printed by the Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperative Description of the Invention (41) 1877..1870(91) 793..800(90) 1356..1363(90) 1815..1808(90) 317.324(90) M00285 1734" 1746(95) TCF11 TCFl 1/KCR- Fi/Nrfl Homogeneous Dimer 1110..1122(91) 13..1(90) M00134 302..320(95) HNF-4 Hepatic Nuclear Factor 4 ---- M00077 1933..1941(95) GATA- 3 GATA-binding factor 3 _____ M00096 1827..1819(95) Pbx-I Pbx-1 __- M00141 451..459(94) Lyf-1 LyF-1 ___ M00199 1797..1789(94) AP-I AP -1 binding site 1789..1797(91) M00174 1788..1798 (94) AP-1 activator protein 1 __- M00076 1483..1492 (93) GATA-2 GATA-binding factor 2 1932..1941(93) 1701..1692(92) 982..991(92) M00278 1933..1941(93) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Lm〇2 complex, half site 2 M00099 1813..1798(92) S8 S8 1109..1124(90) MQ0294 1551-1539(92) HFH-8 HNF-3/Fkh Homolog-8 M00223 542..534(92) STATx Message Transducer and Transcription Activator M00073 36..46(92) deitaEFl deitaEFl M00271 1955·· 1960(92) AML-1 a Small livestock-factor AML-1 132..127(92) M00137 335.323(91) Oct-1 Dv polymer factor 1 M00042 644..653(91) Sox-5 SoX'5 1829 ..1820(90) M00289 379.391(91) HFH-3 HNF-3/Fkh Homolog 3 (= Freac^6) M00183 1025..1034(90) c-Myb c-Myb M00240 239..233(90) Nkx-2.5 Source Power Factor Factor Nkx-2.5/Csx, Ironsmiths M00188 1788..1798(90) AP-1 / Right 1C Protein 1 -- M00302 836..825(90) NF-AT Purification Nuclear factor of tau cells"~~---- M00128 92..104(90) GATA-1 GATA-binding factor 1 ---~~~ M00184 269..278(90) MyoD myocyte determinant &quot ; —- M0 0147 M00087 164.. 155(90) 979.. 990(90) HSF2 Ik-2 Heat Rest Factor 2 --- Dcaros 2 —---- M00172 1788..1798(90) AP-1 Activator White I —------ M00221 1860..1850(90) SREBP- 1 Due to drunken control components - combined with white 1 ------- '--J Table 14 · DUB regulation of hDUB 8.2 (or The main junction of the putative transcription factor in the promoter region (position is expressed in nucleotides)

Transfac丨 position (score) Name -43- This paper size applies to China National Standard (CNS) A4 specification (210 X 297 mm)

A7 B7 1312008 V. Description of invention (42) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed M00241 1804..1811(100) Nkx-2.5 homologous work site factor Nkx-2.5/Csx. foreign blacksmith homogenate Μ0024Θ 556. .562(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, foreign blacksmith homogenate 1354..1360(90) 301..307(90) M00096 1342..1350(100) Pbx-1 Pbx- l 1831..1823(%) M00271 702..707(100) AML-la Small Livestock-Factor AML-1 1955..1960(92) 170..175(92) M00050 104..111(100) E2F E2F — M00I48 1271..1277(100) SRY Sex determination region Y gene product 1572..1578(92) 1345..1351(90) 1719..1713(90) 1828..1822(90) 1634..1628(90 M00272 472..463(97) P53 Tumor suppressor p53 463..472(97) 295..286(91) M00075 1842..1851(97) GATA-1 GATA·binding factor 1 1893..1902 ( 93) 1380..1371(92) 1740..1749(90) M00253 1072..1065(96) cap transcription start message 1798..1805(95) 1897..1890(95) 769..776( 92) 1701..1708(92) 1881..1874(91) 497..504(90) 1819..1812(90) 21-28(90) M00285 1738..1750(95) TCF11 TCF11/KCR-Fl /Nrf 1 with Dimer 366.378(93) M00077 1933..1941(95) GATA-3 GATA-binding factor 3 M00147 148..139(94) HSF2 Heat shock factor 2 139..148(92) M00199 1801..1793(94 AP-1 AP-1 binding site 1037..1029(94) 1793..1801(91) 1029..1037(90) M00174 1792..1802(94) AP-1 activated prion protein 1 1038..1028 (92) M00074 1247..1259(93) c-Ets-1 (P54) c-Ets-l(p54) M00211 702..710(93) Poly retrovirus polyadenylate downstream element 4 .. 1531. .1539(91) M00124 1339..1353(93) Pbxlb homologous functional site factor Pbx-l _ -44- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 V. Invention Description (43) Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed -45- M00278 1933..1941(93) Lmo2 binds to Ta丨-1, E2A protein, and GATA-1 Lmo2 complex, half site 2 M00052 1261..1252(93) NF-zcB NF-A:B(p65) M00076 1932.. 1941(93) GATA-2 GATA-binding factor 2 837..828(90) M00099 1817..1802(92) S8 S8 M00101 987..981(92) CdxA CdxA M00042 1635..1626(92) Sox-5 Sox-5 M00254 1835..1824(91) CCAAT Fine running Viral CCAAT匣M00008 252..243(91) Spl Excitation protein 1 1323..1314(91) M00227 933..941(90) v-Myb v-Myb M00141 1328..1320(90) Lyf-1 LyF- 1 M00183 1033..1042(90) c-Myb c-Myb M00001 654..665(90) MyoD myocyte determinant M00188 1792..1802(90) AP-l activator protein 1 M00184 23..14( 90) MyoD myocyte determinant M00172 1792..1802(90) AP-l activator protein 1 M00221 1864..1854(90) SREBP-1 sterol regulatory element-binding protein 1 M00037 871..861(90) NF-E2 NF-E2 p45 M00053 1261..1252(90) c-Rel c-Rel M00249 823..835(90) CHOP- C/EBPa CHOP舆C/ΕΒΡα heterodimer M00302 1248..1259( 90) Nuclear factor of NF-AT-activated T cells This paper scale applies to Chinese National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention (44) Table 15. DUB regulation of hDUB 8.3 (or Promoter) The main structure of the putative transcription factor in the region (position is expressed in nucleotides) Printed by the Intellectual Property Intelligence Bureau employee consumption cooperative

Transfac Position (Score) Name Description M00348 71..80(100) GATA-2 GATA-binding factor 2 N400349 71..80(100) GATA-2 GATA-binding factor 2 MOO 148 990..984(100) SRY Sex Determine the region Y gene product 1654..1648(100) 813..807(96) 917..911(96) 1848..1854(92) 1840..1846(92) 1826..1832(92) 1504.. 1498(92) 1391..1397(90) 680..686(90) 2094..2088(90) 368..362(90) 721.715(90) 1002..996(90) 1900..1894(90) 274..280(90) M00350 71..80(100) GATA-3 GATA-binding factor 3 MOO ΙΟΟ 595..589(100) CdxA CdxA 1400..1394(100) M00I01 595..589(99) CdxA CdxA 1400..1394(99) 803..797(98) 1527..1521(98) 1705..1711(92) 699..693(92) 934..940(92) 43..37(92) 495..501(92) 397..403(92) M00141 1274..1266(98) Lyf-1 LyF-1 M00347 71..80(97) GATA-1 GATA-binding factor 1 M00272 1563..1572( 96) P53 tumor suppressor p53 M00253 1128..1135(96) cap transcription initiation cap message 136..143(96) 2064..2071(95) 457..464(95) 2163..2156(95) 721..728(94) 660..653(94) 1782..1775(92) 312..319(92) 1622..1615(91) -46- Ben Zhang scale applies China National Standard (CNS) A4 specification (210 x 297 mm) A7 B7 1312008 V. Invention description (45) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative print 535..542(91) 2085..2078(91 ) 2147..2140(91) i350'.i357(9i) 768..775(90) 295..302(90) M00285 2004..2016(95) TCF11 TCFl l/KCR-Fl/Nrfl Homodimer 1410..1422(91) M00254 2101..2090(95) CCAAT Cellular and viral CCAAT匣M00130 1647..1658(95) HFH-2 HNF-3/Fkh homogen 2 M00054 442..433(95) NF-λΒ NF-/CB M00077 2203..2211(95) GATA-3 GATA-binding factor 3 M00052 442..433(94) NF-fcB NF-/cB(p65) M00203 73..83(94) GATA -X GATA binding site M00199 2067..2059(94) AP-i AP-1 binding site 2059..2067(91) 1984..1992(90) M00174 2058..2068(94) AP-1 activator Protein 1 M00075 895..886(94) GATA-1 GATA-binding factor 1 2159..2168(93) 1082..1073(93) 1971..1962(92) 2006..2015(90) M00053 442.. 433(94) c-Rel c-Rel M0024I 2070..2077(94) Nkx-2.5 Homologous Functional Site Factor Nkx-2.5/Csx. Ironsmith Homogeneous M00076 716..707(94) GATA-2 GATA- Factor 2 1599..1608(92) 957..966(92) 895..886(91) 1971..1962(91) M00106 627..636(94) CDP-like homologous functional site protein M00033 731..718(94) p300 p300 M00227 2139..2131(94) v-Myb v-Myb M00158 295..282(93) COUP-TF COUP/HNF»4 Heterodimer M00I62 2070..2083(93 Oct-I octamer-binding factor 1 M00134 280..298(93) HNF-4 hepatic nuclear factor 4 M00032 226,.235(93) c-Ets-l(p54) c-Ets-l(p54) M00117 1080..1067(92) C/EBPbeta CCAAT/promoter binding protein 泠M00223 520..512(92) STATx message transducer and transcriptional activator M00042 1901..1892(92) Sox-5 Sox-5 622 ..631(91) M00073 13-23(92) dEFl 5EF1 M00099 2083..2068(91) S8 S8 2066..2081(90) 1409..1424(90) M00289 1647..1659(91) HFH-3 HNF-3/Fkh homogenate 3 (= Freac-6) 357..369(91) M00147 752..743(91) HSF2 heat shock factor 2 -47- This paper scale applies to China National Standard (CNS) A4 specification ( 210x297 mm) 1312008 V. Inventions (46) 142..133(90) M00208 443..432(90) NF-/CB NF-/CB binding site M00217 115..108(90) USF USF binding site Point M00I83 1325..1334(90) c-Myb c-Myb M00240 217..211(90) Nkx-2.5 homologous functional site factor Nkx-2.5/dsx. Artichoke E homologue MOOI88 2058..2068(90) AP-1 activator protein i __ M00I84 1955..1964(90) MyoD myocyte determinant 247..256(90) M00087 954..965(90) Ik-2 Ikaros 2 M00128 70..82(90) GATA-1 δΑΤΑ-binding factor 1 " - M00172 2058..2068(90) AP-1 Activator protein I M00145 1339..1354(90) Bm-2 POU factor Brn-2 M00062 1217..1205(90) IRF-L Interferon Regulatory Factor 1 M00194 444., 431(90) NF-/CB NF-ΛΒ Table 16. The main structure of the putative transcription factor in the DUB regulatory (or promoter) region of hDUB 8.4 (position with nucleoside Acid said) Ministry of Economic Affairs, Intellectual Property Bureau, employee consumption cooperative, printing

Transfac position (score > name description M00100 1105..1099(100) CdxA CdxA M00241 1800..1807(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx. 浑铠 同 homomorphism M00148 1020..1014 (100) SRY sex determining region Y gene product 970..976(100) 947..941(96) 1550..1556(92) 843..837(90) 707..713(90) 1362..1356( 90) 1719..1713(90) 1824..1818(90) 395.389(90) 748..742(90) 300.306(90) M00101 1105..1099(99) CdxA CdxA 833..827(98) 1385. .1379(98) 1409..1415(92) 964..970(92) 726..720(92) 424..430(92) M00075 1838..1847(97) GATA-1 GATA-binding factor 1 1889 ..1898(93) 925..916(93) 1736..1745(90) M00272 1267..1276(96) P53 Tumor suppressor P53 M00253 161..168(96) cap Transcription initiation cap message 1794 ..1801(95) 484..491(95) -48- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention Description (47) Ministry of Economic Affairs Intellectual Property Bureau Employee Consumption Cooperative Printed 1893..1886(95) 1452..1459(94) 798..805(94) 748..755(94) 957..964(94) 1486..1479(92) 338.. 345 (9 2) 1697..1704(92) 562..569(91) 1877..I87CK91) 1815..1808(90) 321..328(90) 1326..1319(90) 1299..1306(90) MOOO % 1827..1819(96) Pbx-l Pbx-l M00285 1734..1746(95) TCF11 TCFl l/KCR-Fl/Nrf 1 Homogeneous dimer 1115..1127(91) M00077 1933..1941(95 GATA-3 GATA-binding factor 3 M00054 469..460(95) NF-λΒ NF-λΒ M00141 456..464(94) Lyf-I LyF-1 M00134 306..324(94) HNF-4 Hepatic nucleus Factor 4 M00052 469..460(94) NF-λΒ NF-/cB(p65) M00199 1797..1789(94) AP-1 AP-l binding site 1789..1797(91) M00174 1788..1798( 94) AP-i activator protein 1 M00053 469..460(94) c-Rel c-Rel M00033 758..745(94) p300 p300 808..795(92) M00158 321..308(93) COUP- TF COUP/HNF-4 heterodimer M00278 1933..1941(93) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Lmo2 complex, half site 2 M00076 1932..1941(93) GATA-2 GATA-binding factor 2 1303,.1312(92) 743..734(92) 987..996(92) 925..916(90) M00099 1813..1798(92) S8 S8 1114..1129 (90) M00104 28..37(92) CDP-like homologous functional site protein M00223 547..539 ( 92) STATx message transducer and compilation activator M00271 1955..1960(92) AML-la small livestock-factor AML-1 M00254 1831..1820(91) CCAAT cellular and viral CCAAT匣M00042 649..658 (91) Sox-5 Sox-5 M00289 384..396(91) HFH-3 HNF-3/Fkh Homolog 3 (= Freac*6) M00302 1384..1395(91) NF-AT Activates the nucleus of T cells Factor 841..830(90) M00155 367.382(91) ARP-1 Apolipoprotein AI regulatory protein 1 M00208 470..459(90) NF-/CB NF-/CB binding site-49- This paper size applies China National Standard (CNS) A4 Specification (210x297 mm) A7 B7 1312008 V. Description of Invention (48)

M00183 1030..1039(90) c-Myb c-Myb M00240 243..237(90) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate 1454..1448(90) M00188 1788. .1798(90) API Activation Pin Protein 1 M00083 47..40(90) MZF1 MZF1 M00184 273..282(90) MyoD Muscle Embryo Determining Factor M00147 167..158(90) HSF2 Heat Shock Factor 2 M00087 984 ..995(90) Ik-2 Ikaros 2 M00172 1788..1798(90) AP-i activator protein 1 M00221 1860..1850(90) SREBP-i sterol regulatory element-binding protein 1 M00194 471..458 (90) NF-zcB NF-/CB Table 17. The main structure of the putative transcription factor in the DUB silk control (or promoter) region of hDUB 8.5 (position is expressed in nucleotides) Ministry of Economic Affairs Intellectual Property Office employee consumption cooperative system

Transfac position (score > name description M00100 1105..1099(100) CdxA CdxA M00241 1800..1807(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homomorphism M00271 27.32(100) AML -la small livestock-factor AML-1 1955..1960(92) M00148 1020..1014(100) SRY sex-determining region Y gene product 970..976(100) 947-941(96) 1553..1559(92 100..106(92) 141..135(90) 843..837(90) 1365..1359(90) 1719..1713(90) 1824..1818(90) M00101 1105..1099(99 CdxA CdxA 833..827(98) 1234..1228(98) 1388..1382(98) 1300..1294(93) 1412..1418(92) 964..970(92) 1682..1688( 91) 1688..1682(91) M00278 618..610(98) Lmo2 binds to Tal-1, E2A protein, and GATA-丨 Lmo2 complex, half-site 2 1933..1941(93) M00075 1838 ..1847(97) GATA-1 GATA-binding factor 1 619..610(97) 1701..1692(95) 1889..1898(93) 903..894(91) 1736..1745(90) - 50- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention description (49) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed M0005 4 442..451(96) NF-/CB NF-zcB 451..442(91) M00253 175,.168(96) cap transcription start message 1794..1801(95) 1893..1886(95 ) 1455..1462(94) 798..805(94) 629..636(94) 1489..1482(92) 433..426(92) 1329-1322(91) 1877..1870(91) 921 ..928(91) 957..964(90) 1815..1808(90) 1302..1309(90) M00272 1270..1279(96) P53 Tumor suppressor p53 M00Q96 1827..1819(96) Pbx- 1 Pbx-i M00285 1734..1746(95) TCFil TCFll/KCR-Fl/Nrfl Homogeneous Dimer 1115·· 1127(91) 1490..1478(91) M00076 619..610(95) GATA-2 GATA - binding factor 2 1932..1941 (93) 1701.. 1692(92) 1306..1315(92) 987..996(92) M00077 1933..1941(95) GATA-3 GATA-binding factor 3 618. .610(92) M00L99 1797..1789(94) AP-1 AP-1 binding site 1789..1797(91) M00174 1788..1798(94) AP-1 Activator protein 1 M00083 566..559( 93) MZF1 M2F1 M00099 1813..1798(92) S8 S8 1114..1129(90) M00208 441..452(92) NF-/cB NF-/CB binding site M00033 808..795(92) p300 P300 M00227 1677..1669(91) v-Myb v-Myb M00254 1831..1820(91) CCAAT Cellular and viral CCAAT匣MO OI83 166..157(91) c-Myb c-Myb 1030..1039(90) M00267 102..89(91) XFD-1 M00240 1457..1451(90) Nkx-2.5 homologous functional site factor Nkx- 2.5/Csx, hip-caster homogenate 296..302(90) Xenopus fork-shaped head functional site factor 1 MOOI88 1788..1798(90) AP-1 activator protein 1 ^ M00302 841..830(90) NF- AT-activated T cell nuclear factor M00087 984..995(90) Ik-2 Ikaros 2 -51- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention ( 50) M00172 1788..1798(90) AP-i activator protein 1 M00003 965..956(90) v-Myb v-Myb M00221 1860..1850(90) SREBP-i sterol regulatory element-binding protein 1 M00053 441..450(90) c-Rei c-Rel Table 23. Main structure of the putative transcription factor in the DUB regulatory (or promoter) region of hDUB 8.11 (position expressed in nucleotides) Ministry of Economic Affairs Intellectual Property Office staff Printed by consumer cooperatives

Transfac position (fraction) Name Description M00100 1105..1099(100) CdxA CdxA M00241 1800..1807(100) Nkx-2.5 Homologous functional site factor Nkx-2.5/Csx, blacksmith homogenizer M00148 1020..1014(100 SRY Sex Determination Region Y Gene Product 970..976(100) 947..941(96) 1550..1556(92) 843..837(90) 707..713(90) 1362..1356(90) 1719..1713(90) 1824..1818(90) 395..389(90) 748..742(90) 300..306(90) MOO 101 1105..1099(99) CdxA CdxA 833..827 (98) 1385..1379(98) 1409..1415(92) 964..970(92) 726..720(92) 424..430(92) M00075 1838..1847(97) GATA-1 GATA -binding factor 1 1889..1898(93) 925^916(93) 1736..1745(90) M00272 1267..1276(96) p53 tumor suppressor p53 M00253 161..168(96) cap transcription initiation Cap message 1794..1801(95) 484..491(95) 1893..1886(95) 1452..1459(94) 798..805(94) 748..755(94) 957..964(94 ) 1486..1479(92) 338..345(92) 1697..1704(92) 562..569(91) -52- 'Installation — • Order · Line · This paper scale applies to China National Standard (CNS) )A4 size (210x297 mm)

1312008 I V. Description of invention (51) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed 1877..1870(91) 1815..1808(90) 321..328(90) 1326.· 1319(90) 1299.. 1306(90) M00096 1827..1819(96) Pbx-i Pbx-1 M00285 1734..1746(95) TCFI1 TCF11/KCR-Fl/Nrf I Homogeneous Dimer 1115..1127(91) M00077 1933.. 1941(95) GATA-3 GAT binding factor 3 M00054 469..460(95) NF-/CB NF-/CB MOOI41 456..464(94) Lyf-i LyF-1 M00134 306..324(94) HNF -4 Hepatic nuclear factor 4 M00052 469..460(94) NF-zcB NF-/cB(p65) M00199 1797..1789(94) AP-1 AP-1 binding site 1789..1797(91) M00174 1788 ..1798(94) AP-1 Activator Protein 1 M00053 469..460(94) c-Rel c-Rel M00033 758,.745(94) p300 p300 808..795(92) M00158 321..308( 93) COUP-TF C0UP/HNF4 heterodimer M00278 1933..1941(93) Lmo2 binds to Tal-1, E2 A protein, and G ΑΤΑ-1 Lmo2 complex, half site 2 M00076 1932-1941 (93) GATA-2 GATA-binding factor 2 1303..1312(92) 743..734(92) |987..996(92) 9^..916(90) M00099 1813..1798(92) S8 S8 1114..1129(90) M00104 2S..37(92) CDP Excised homologous functional site protein MQQ223 547..539(92) STATx message transducer and transcriptional activator M00271 1955..1960(92) AML-la small livestock-factor AML-1 M00254 1831..1820(91 CCAAT Cellular and viral CCAAT匣M00042 649..658(91) Sox-5 Sox-5 M00289 384.396(91) HFH-3 HNF-3/Fkh homogenate 3 (= FreacA) M00302 1384..1395 (91 NF-AT activates T cell nuclear factor 841..830(90) M00155 367..382(91) ARP-1 alipoprotein AI regulatory protein 1 M00208 470..459(90) NF-/CB NF- /CB binding site M00183 1030,.1039(90) c-Myb c-Myb M00240 243..237(90) Nkx-2,5 homologous functional site factor Nkx-2.5/Csx, blacksmith homomorphism 1454.. 1448(90) M00188 1788..1798(90) AP-1 Activator Protein 1 M00083 47..40(90) MZF1 MZF1 M00184 273..282(90) MyoD Muscle Embryo Determining Factor M00147 167..158(90 HSF2 heat shock factor 2 . -53- This paper size is applicable to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 V. Invention description (52) M00087 984..995(90) Ik-2 Ikaros 2 M00172 1788..1798(90 AP-L activator protein i M00221 1860..1850(90) SREBP-i sterol regulatory element-binding protein 1 M00194 471..458(90) NF-zcB ΝΡ-ΛΒ Table 丨 9. hDUB 8.7 DUB regulation The main structure of the putative transcription factor in the (or promoter) region (position is expressed in nucleotides) Printed by the Intellectual Property Intelligence Bureau employee consumption cooperative

Transfac position (score > name description M00349 94..103(100) GATA-2 GATA-binding factor 2 M00348 94..103(100) GATA-2 GAT A-binding factor 2 M00241 1800..1807(100) Nkx -2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate M00100 1102..1096(100) CdxA CdxA M00148 1017..1011(100) SRY sex determining region Y gene product 967..973(100) 704 ..710(100) 944..938(96) 1550..1556(92) 840..834(90) 1362.,1356(90) 1719..1713(90) 1824..1818(90) 392. .386(90) 745..739(90) 298..304(90) M00350 94..103(100) GATA-3 GATA·binding factor 3 M00101 1102..1096(99) CdxA CdxA 830..824( 98) 1231..1225(98) 1385..1379(98) 1297..1291(93) 1409..1415(92) 723..717(92) 961..967(92) 66..60(92 421..427(92) 1682..1688(91) 1688..1682(91) M00203 96..106(98) GATA-X GATA binding site M00347 94..103(97) GATA-1 GATA- Binding factor 1 M00075 1838..1847(97) GATA-1 GATA-binding factor 1 1701.. 1692(95) 1889..1898(93) 900..891(91) 138..129(90) 1736.. 1745(90) M00158 319..306(96) COUP-TF COUP/HNF4 Heterodimer M002 72 1267..1276(96) p53 Tumor suppressor p53, -54- This paper scale applies to Chinese National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. Description of invention (53) Ministry of Economic Affairs Property Bureau Staff Consumer Cooperative Printed M00I34 304..322(96) HNF-4 Liver Factor 4 M00096 1827..1819(96) Pbx-I Pbx-ί M00285 1734..1746(95) TCF11 TCFI 1/KCR- Fi/Nrfl Homogeneous Dimer 1112..1124(91) 1487.1475(91) 13..1(90) M00077 1933..1941(95) GATA-3 GATA-binding factor 3 M00253 1794..1801(95) cap The transcription start cap message 481.488 (95) 1893..1886 (95) 1452..1459 (94) 795..802 (94) 656..649 (93) I486.· 1479(92) 1326..1319( 91) 559..566(91) 1877..1870(91) 745..752(91) 918..925(91) 954..961(90) 1815..1808(90) 1299..1306(90 M00054 466..457(95) NF-/CB NF-/CB M00146 165..156(94) HSF1 heat shock factor 1 M00147 165..156(94) HSF2 heat shock factor 2 156..165(94) M00141 453,.461(94) Lyf-1 LyF-1 M00052 466..457(94) NF-/CB NF-/cB(p65) MOO 199 1797,.1789(94) AP-1 AP-1 Binding Site Point 1789..1797(91) M00174 1788'·1 798(94) AP-1 Activator Protein 1 M00053 466..457(94) c-Rel c-Rel M00278 1933..1941(93) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Lmo2 Complex, half site 2 M00076 1932..1941 (93) GATA-2 GATA-binding factor 2 1701..1692(92) 1303..1312(92) 984..993(92) M00099 1813..1798( 92) S8 S8 1111..1126(90) M00184 271..280(92) MyoD myocyte determinant M00223 544..536(92) STATx message transducer and transcriptional activator M00073 36..46(92) i5EFl 5EF1 M00033 805..792(92) p300 p300 M00271 1955..1960(92) AML-Ia Small Livestock-Factor AML-1 M00254 1831..1820(91) CCAAT Cellular and Viral CCAAT匣 M00277 281. .270(91) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Lmo2 complex, half site 2 This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Description of invention (54)

M00289 381..393(91) HFH-3 HNF-3/Fkh Homolog 3 f:= Freac-6) '-1 M00208 467..456(90) NF-/CB NF-/CB Binding Site-- -- M00183 1027..1036(90) c-Myb c-Myb M00240 241..235(90) Nkx-2.5 Homologous functional site factor Nkx-2.5/Csx, foreigner homogenizer ~ 1454..1448(90) M00188 1788..1798(90) AP-1 Activator Protein 1 _ M00302 838..827(90) NF-AT Nuclear Factor for Activated T Cells MOQ128 93·· 105(90) GATA-1 GATA-binding Factor 1 M00087 981..992(90) Ik-2 Ikaros 2 M00172 1788..1798(90) AP-1 Activator Protein 1 --- M00042 69..78(90) Sox-5 Sox-5 M00003 962..953( 90) v-Myb v-Myb M00221 1860..1850(90) SREBP-1 sterol regulatory element··binding 蚤白1 — M00194 468..455(90) NF-/CB NF-/CB Table 20. hDUB The main structure of the putative transcription factor in the UD regulatory (or promoter) region of 8.8 (position is expressed in nucleotides)

Transfac Position (Score) Name Description - M00148 1020..1014(100) SRY Sex Determination Region Y Gene Product 970..976(100) 947..941(96) 1550..1556(92) 843..837(90 ) 707..713(90) 1362..1356(90) 1719..1713(90) 1824..1818(90) 395.389(90) 748..742(90) 300..306(90) M00241 1800. .1807(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate M00100 1105..1099(100) CdxA CdxA MOO 101 1105..1099(99) CdxA CdxA 833..827(98 ) 1385..1379(98) 1409..1415(92) 964..970(92) 726..720(92) 424..430(92) M00075 1838..1847(97) GATA-1 GATA-Combination Factor 1 1889..1898(93) 925..916(93) 1736..1745(90) M00272 1267..1276(96) P53 Limb suppressor p53 M00253 161..168(96) cap transcription initiation Cap message 1794..1801(95) 484..491(95) -56- Ministry of Economic Affairs Intellectual Property Bureau Employees Consumption Co., Ltd. Printed on this paper scale Applicable to China National Standard (CNS) A4 specification (210x297 mm) 1312008 A7 B7 V. Description of invention (55) Printed by the Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperatives 1893..1886(95) 1452..1459(94) 7 98..805(94) 748..755(94) 957..964(94) 1486..1479(92) 338..345(92) 1697..1704(92)^62..569(91) 1877..1870(91) 1815..1808(90) 321.328(90) 1326..1319(90) 1299..1306(90) Μ00096 1827..1819(96) Pbx-I Pbx-1 Μ00285 1734.. 1746(95) TCF1I TCFU/KCR-Fl/Nrfl homodimer 1115..1127(91) Μ00077 1933..1941(95) GATA-3 0 octagonal _binding factor 3 Μ00054 469..460(95) NF -/CB NF-/CB Μ00141 456..464(94) Lyf-1 LyF-1 Μ00134 306..324(94) HNF4 Hepatic nuclear factor 4 Μ00052 469..460(94) NF-/cB NF-«B (p65) Μ00199 1797..1789(94) AP-1 AP-1 binding site 1789..1797(91) Μ00174 1788..1798(94) AP-1 Activator protein 1 Μ00053 469..460(94) c-Rel c-Rel Μ00033 758,.745(94) p300 p300 808..795(92) Μ00158 321.308(93) COUP-TF COUP/HNF4 Heterodimer Μ00278 1933..1941(93) Lmo2 binds to Tal -1, E2 A protein, and G ΑΤΑ-i Lmo2 complex, half site 2 Μ 00076 1932..1941 (93) GATA-2 GATA-binding factor 2 1303..1312(92) 743..734( 92) 925..916(90) Μ00099 1813..1798(92) S8 S8 1114..1129(90) Μ00104 28..37(92) CDP-like homologous functional site protein Μ00223 547..539(92) STATx message transducer and transcriptional activator Μ00271 1955..1960(92) AML-la small livestock-factor AML -1 Μ00254 1831-1820(91) CCAAT cellular and viral CCAAT匣Μ00042 649..658(91) Sox-5 Sox-5 Μ00289 384..396(91) HFH-3 HNF-3/Fkh homogenate 3 (= Freac*6) Μ00302 1384..1395(91) Nuclear factor of NF-AT-activated T cells 841..830(90) Μ00155 367..382(91) ARP-1 apolipoprotein AI regulatory protein 1 . Μ00208 470..459(90) NF-/CB NF-/CB binding site Μ00183 1030..1039(90) c-Myb c-Myb -57- This paper scale applies to China National Standard (CNS) A4 specification (2 ] 0χ297 mm) A7 B7 1312008 V. Inventive Note (56) M00240 243..237(90) Nkx-2.5 Synergy functional part factor Nkx-2_ VCsx, foreign suspicion® clear version 1454..1448(90) M00188 1788..1798(90) AP-1 Activator $白1 —- M00083 47-40(90) MZF1 MZF1 _ ' ----- M00184 273..282(90) MyoD Muscle Embryo Determinant --- -M00147 167..158(90) HSF2 Heat shock factor 2 ------ M00172 1788..1798(90) AP-1 Activator protein 1 --- M00221 1860..1850(90) SREBP-1 Sterol regulatory element-binding 孓白1 ' -- M00194 471..458(90) NF-/CB NF-/CB ---- Table 21. Binding putative transcription in the DUB regulatory (or promoter) region of hDUB 8.9 The main surname of the factor (position is expressed in nucleotides) ~ Printed by the Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperative

Transfac Location (score) Name Description ___ M00148 991..997(100) SRY Sex Determination Region Y Gene Product -- 1040..1034(100) 967.961(96) 1576..1582(92) 1550..1556(92 ) 1385..1379(90) 1824..1818(90) 13..19(90) 415..409(90) 727..733(90) MOO HU 1125..1119(100) CdxA CdxA . 1254. .1248(98) 1167..1161(97) 542..548(97) 884..878(94) 1432..1438(93) 743..737(92) 89..83(92) 592.. 586(92) 1280..1286(92) 444..450(92) 1682..1688(91) 1688..1682(91) M00083 779..772(100) MZF1 MZF1 68..61(90) M00241 1800..1807(100) Nkx-2.5 homology and functional site factor Nkx-2.5/Csx. Ironsmith homogenate M00272 1290..1299(96) P53 tumor suppressor P53 M00253 182.189(96) cap transcription initiation cap Message 1794..1801(95) 1893..1886(95) 1475..1482(94) 768..775(94) 1506..1499(93) 1349..1342(91) -58- This paper size applies China National Standard (CNS) A4 Specification (210 X 297 mm) 1312008 A7 B7 V. Description of Invention (57) Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 582..589(91) 351..358(90) 1815 .-1808(90) 342..3 49(90) 1877..1870(90) 504.511(90) 1322..1329(90) M00100 1125..1119(96) CdxA CdxA M00042 742..751(96) Sox-5 Sox-5 669..678 (92) 726..735(92) M00096 1827..1819(96) Pbx-1 Pbx-1 M00077 1933,.1941(95) GATA-3 GATA-binding factor 3 M00045 1877..1888(95) E4BP4 E4BP4 M00054 489..480(95) NF-/CB NF-λΒ M00141 476..484(94) Lyf-1 LyF-1 M00052 489..480(94) NF-/CB NF-/cB(p65) M00I99 1797 ..1789(94) AP-I AP-1 binding site 1789., 1797(91) M00174 1788..1798(94) AP-1 Activator protein 1 M00053 489..480(94) c-Rel c- Rel M00278 1933..1941(93) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Lm〇2 complex, half site 2 M00076 1932..1941 (93) GATA-2 GATA·binding factor 2 1007..1016(92) 1701..1692(91) M00285 1734..1746(92) TCF11 TCFU/KCR-Fl/Nrfl Homogeneous one "Material 1135..1147(91) M00099 1813-1798(92) S8 S8 1134..1149(90) M00104 49-58(92) CDP-like homologous functional site protein _____ M00223 567..559(92) STATx message transducer and transcriptional activation 剤______—M00075 1701. .1692(92) GATA-1 GATA-Combination Factor 1 -------- 161..152(90) 1736..1745(90) M00348 316.325(92) GATA-2 GATA-binding factor 2 ----- M00I09 873"886(91) C /EBPiS 0: 八八17促子结会荇6/9 ------- M00254 1831..1820(91) CCAAT Cellular and viral CCAAT» ----- M00203 318.328(91) GATA- X GATA binding site I·'1·M00350 316.325(91) GATA-3 GATA-binding factor 3 ______^" M00289 404..416(91) HFH-3 HNF-3/Fkh homogenate 3 (= Rreac- 6)________一" M00113 833..822(90) CREB cAMP-inductive element combined with Fanbaiyi^" M00249 1165..1153(90) CHOP-C/EBPa CHOP and C/ΕΒΡα 4 dimerization ―--&C; --Factory M00190 1263.. 1250(90) C/EBP (ΧΛΑΤ/promoter combination cause + ___—^--^ M00349 316..325(90) GATA-2 GATA-binding factor 2 ___-一-- ΜΟΟΠ6 1263..1250(90) C/EBPa CCAAT/promoting sub-conclusion white" ^' -59- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm)

1312008 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (58) M00183 1050..1059(90) c-Myb c-Myb M00240 263..257(90) Nkx-2.5 homologous functional site factor Nkx- 2.5/Csx, foreign iron S homogenate 1477..1471(90) M00188 1788..1798(90) AP-1 activated prion protein l M00302 1407..1418(90) NF-AT activation · τ fine running nuclear factor M00184 293..302(90) MyoD myocyte determinant M00147 188..179(90) HSF2 heat shock factor 2 M00087 1004..1015(90) Ik-2 Ikaros 2 M00172 1788.1798(90) AP-1 activated protein 1 M00221 1860..1850(90) SREBP-1 sterol regulatory element-binding protein 1 M00033 778..765(90) p300 p300 M00194 491..478(90) NF-/CB NF-ftB Table 22. hDUB 8.10 The main structure of the putative transcription factor in the DUB regulatory (or promoter) region (position is expressed in nucleotides) Transfac position (fraction > name description M00I48 1038..1032(100) SRY sex determining region Y gene product 965- 959(96) 1550..1556(92) 725..731(90) 1382..1376(90) 310..316(90) 1715..1709(90) 1824..1818(90) 12..18 (90) 413..407(90) 1630..1 624(90) 319..325(90) M00241 1800..1807(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate M00100 1123..1117(100) CdxA CdxA M00240 1240. .1234(100) Nkx-2.5 homologous functional site factor Nkx-2.5/Csx, blacksmith homogenate 262..256(90) M0014! 474..482(100) Lyf-1 LyF-1 M00101 1123-1117( 99) CdxA CdxA 540..546(97) 1429..1435(94) 982..988(92) 88..82(92) 741..735(92) 442..448(92) M00075 1838.. 1847(97) GATA-1 GATA-binding factor 1 1889..1898(93) 1736..1745(90) M00253 766..773(96) cap transcription initiation cap message V 181..188(96) 1794 ..1801(95) 1893..1886(95) -60- This paper scale applies to China National Standard (CNS) A4 specification (210 x 297 mm). 1312008 A7 B7 V. Description of invention (59) Ministry of Economic Affairs Intellectual Property Bureau Employee Consumption Cooperatives Printed 1210..1217(94) 816..823(94) 1506..1499(92) 357..364(92) 1697..1704(92) 1090.. i 097(91) 1346. .1339(91) 1877..1870(91) 71..78(90) 580..587(90) 1815..1808(90) 340..347(90) 502..509(90) 1319.. 1326(90) M00096 1827..1819(96) Pbx-1 Pbx-1 M002S5 1734..1746(95) TCF11 TCFl I/KCR-Fl/Nrf 1 Homogeneous Dimer 1133..1145(91) 35..23(91) 1089..1101(90) M00134 325..343(94 HNF-4 Hepatic nuclear factor 4 M00199 1797..1789(94) AP-1 AP-1 binding site 1789..1797(91) M00174 1788..1798(94) AP-1 Activator protein 1 M00042 740. .749(94) Sox-5 Sox-5 1631..1622(92) 667..676(91) M00106 672..681(94) CDP-like homologous functional site protein M00158 340..327 (93 COUP-TF COUP/HNF4 Heterodimer M00076 761.752(93) GATA-2 GATA-binding factor 2 1323..1332(92) 1005..1014(92) M00099 1813..1798(92) S8 S8 1132. .1147(90) M00104 48..57(92) CDP-like homologous functional site protein M00223 565..557(92) STATx message transducer and transcriptional activator M00033 826..813(92) p300 P300 M00271 1955..1960(92) AML-la Small Livestock-Factor AML-1 M00254 1831..1820(91) CCAAT Cellular and Viral CCAAT匣M00289 402..414(91) HFH-3 HNF-3/Fkh Homogenous 3 (= Freac*6) M00217 160..153(90) USF USF binding position 黠M00183 1048..1057(90) c-Myb c-Myb M00188 1788..1798(90) AP-1 activator egg 1 MOOOB3 67..60(90) MZF1 MZF1 M00184 292..301(90) MyoD Myocyte determinant M00147 187..178(90) HSF2 Heat shock factor 2 M00087 1002..1013(90) Ik-2 Ikaros 2 M00172 1788..1798(90) AP-1 activator protein 1 M00221 1860..1850(90) SREBP-1 sterol regulatory element-binding protein 1 -61- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. INSTRUCTIONS (60) Table 23. The main structure of the putative transcription factor in the DUB regulatory (or promoter) region of hDUB 8.11 (position is expressed in nucleotides) Property Bureau employee consumption cooperative printing

Transfac position (score > name description M00348 71..80(100) GATA-2 GATA-binding factor 2 M00350 71..80(100) GATA-3 GAT A-binding factor 3 M00349 71..80(100) GATA -2 GATA-binding factor 2 MOO 148 990..984(100) SRY sex determining region Y gene product 1664..1658(100) 813..807(96) _917..911(96) 1858..1864(92 ) 1850..1856(92) 1836..1842(92) 1399..1405(90) 1514..1508(90) 680..686(90) 2104..2098(90) 368.362(90) 721.. 715(90) 1002..996(90) 1910..1904(90) 274..280(90) M00100 595..589(100) CdxA CdxA 1408..1402(100) 1474..1480(96) MOOIOl 595..589(99) CdxA CdxA 1408..1402(99) 803..797(98) 1537..1531(98) 1715..1721(92) 699..693(92) 934..940(92 43..37(92) 495..501(92) M00141 1282..1274(98) Lyf-1 LyF-1 M00347 71..80(97) GATA-1 GAT A-binding factor 1 M00272 1573.. 1582(96) P53 tumor suppressor p53 M00253 1137..1144(96) cap transcription initiation cap message P 136..143(96) 2074..2081(95) 457..464(95) 2173..2166 (95) 721..728(94) 1444..1437(94) 660..653(94) 1792..1785(92) 312..319(92) -62- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention Description (6i) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 1632..1625(91) 535..542 (91) 2095..2088(91) 1358..1365(91) 768..775(90) 295..302(90) 2157..2150(90) M00096 2107..2099(96) Pbx-1 Pbx -I M00285 2014..2026(95) TCF11 TCFI l/KCR-Fi/Nrfl homogenous one 1418..1430(91) M00130 1657..1668(95) HFH-2 HNF-3/Fkh homogen 2 M00077 2213..2221(95) GATA-3 G AT Α·binding factor 3 M00054 442..433(95) NF-/CB NF-«B ... M00052 442.,433(94) NF-/CB NF- /cB(p6S)____ M00203 73.-83(94) GATA-X GATA binding site M00199 2077..2069(94) AP-1 AP-1 binding site 2069..2077(91) 1994..2002( 90) M00174 2068-2078(94) AP-1 activated prion protein 1 1436..1446(90) M00075 895..886(94) GATA-1 GATA-binding factor 1 2169..2178(93) 1091..1082 (93) 1981..1972(92) 2016.^025(90) M00053 442..433(94) c-Rei c-Rel M00241 2080..2087(94) Nlcx-2.5 Homologous Function Clamp Factor Nkx- 2.5/Cs; c. Blacksmith homogenizer M00076 716 ..707(94) GATA-2 GATA-binding factor 2 2212..2221(93) 1609..1618(92) 957..966(92) 895..886(91) 1981..1972(91) M00106 627..636(94) CDP-like homologous functional site protein M00033 731..718(94) p300 P300 M00227 2149..2141(94) v-Myb v-Myb M00158 295..282(93) COUP -TF COUP/HNF4 heterodimer M00162 2080..2093(93) Oct-1 octamer-binding factor 1 M00278 2213., 2221(93) Lmo2 binds to Tal-1, E2A protein, and GATA-1 Complex of Lmo2, half site 2 M00134 280..298(93) HNF-4 Hepatic nuclear factor 4 M00032 226..235(93) c-Ets-l(p54) c-Ets-l(p54) M00172 1436 ..1446(92) AP-1 Activator Protein I 2068..2078(90) M00117 1089..1076(92) C/EBP0 CCA AT/Promoter Binding Protein 泠M00223 520..512(92) STATx Message Transfer Derivation and transcription activator p M00042 1911..1902(92) Sox-5 Sox-5 622..631(91) -63· This paper size is applicable to China National Standard (CNS) A4 specification (210 x 297 mm) 1312008 V. Description of invention (62)

M00073 13..23(92) 5EFI 0EF1 M00I88 1436..1446(91) AP-1 Activation»1 Protein i 2068..2078(90) 1 M00254 2111..2100(91) CCAAT Cellular and diseased CCAAT a M00099 2093.-2078(91) S8 S8 2076..2091(90) 1417..1432(90) M00289 1657..1669(91) HFH-3 HNFO/Fkh Homogenous 3 (= 357..369(91 M00147 752..743(91) HSF2 Heat gram factor 2 142..133(90) M00208 443..432(90) NF-λΒ NF-/CB binding site M00217 115..108(90) USF USF Binding site M00183 1333·. 1342(90) c-Myb c-Myb M00173 1436..1446(90) AP-1 activated prion protein 1 M00240 217..211(90) Nkx-2.5 诼 诼 觝 觝 觝 Nkx -2.5/Csx, blacksmith homogenate M00184 1965..1974(90) MyoD myocyte determinant 247..256(90) M00087 954..965(90) Ik-2 Ikaros 2 M00128 70..82(90 GATA-I GATA-binding factor 1 M00145 1347..1362(90) Bm-2 POU factor Bm-2 M00194 444..431(90) NF-ΛΒ NF-/CB References: 1. Baek, KH, Mondoux , MA, Jaster, R., Fire-Levin, E., and D'Andrea, AD (2001). 5 DUB-2A, a new member of the DUB subfamily of hematopoietic deubiquitinati Ng enzymes,

Blood 98. 636-42. 2. Jaster, R., Baek, KH, and D'Andrea, AD (1999). Analysis of cis-acting sequences and trans-acting factors regulating the interleukin-3 response element of the DUB- 1 gene, Biochim Biophys Acta 1446, 308-16. 10 3. Jaster, R., Zhu, Y., Pless, M., Bhattacharya, S., Mathey-Frevot, B., and D'Andrea, A. D - (1997). JAK2 is required for induction of the murine DUB-1 gene, Mol Cell Biol i7, 3364-72· 4. Migone, TS* Humbert» M., Rascle, A., Sanden* D., DrAndrea, A., Johnston, JA, Baek, KH, Mondoux, MA, Jaster, R., Firc-Levint E., et al. (2001). The deubiquitinating enzyme DUB-2 prolongs cytokine-induced signal transducers and activators of 15 and suppresses apoptosis following cytokine withdrawal. Blood 98^ 1935-41. 5. Zhu, Y., Carroil, M., Papa, FR, Hochstrasser, M., and D'Andrea, AD (1996a). DUB-l, a deubiquitinating enzyme with growth-suppressing activity, Proc Natl Acad Sci USA 9J, 3275-9. Cooperatives printed 6. Zhu, Y., Lambert, K., Corless, C., Copeland, NG, Gilbert, DJ, Jenkins, NA, and 20 D'Andrca, AD (1997). DUB-2 is a member of a novel family of cytokine-induciWe deubiquitinating enzymes, J Biol Chem 272* 51-7- 7. Zhu, Y., Pless, M., Inhom, R., Mathey-Prevot, B., and D*Andrea, AD ( 1996b). The murine DUB-1 gene is specifically induced by the betac subunit of interlcukin-3 receptor, Mol Cell Biol 76,4808-17. -64- This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297) mm) 1312008 A7 B7 V. invention is described in (63) hDUB4_la wipe nucleotide sequence acgaccctgcaacagagcatgcccttctgcattgagcatgcaatcatgaatcacaggcggaggaactgcgagagt gcctacgtcagcccaaggccCgacccgacgatcccagggaccctcgacccaactggccccgccccccgggcccca 5 aacccggactcggcccccccgaagctccggatcctggggcccgcccctggccccgcgtcggaagaccatgggctc gctcctgggccttcctcaaaccctccgcagtccaggcccggcttcccccaggcctccaggcaacgctgcggctcc gcccacgccacggcgcccgaggagaacgcggggacagaactctggctgcagggtCtcgagcgccgcttcctggcg gcgcgcccactgcgctccctcccccggca gagcttagaggcaaagttaagagactcatcagattctgagctgctg cgggatattttgcagaagactgtgaagcatcccgtgtgtgtgaagcacccgccaccagtcaagcatgcccggtgc 10 cttctcCcagaactcatcaaaaagggtgcatctgtggtcaccagcagcacgagggctgtccacacggagcctttg gacgagctgtacgaggtgctggcggagactctgatggccaaggagtccacccagggccaccggagctatttgctg ccctogggaggctcgttcacactttccgagatcacagccatcatctcccatggtactacaggcctggtcacatgg gacgccaccctctaccttgcagaacgggccatcgagaacccagcagccttcactaacaggggtgtcctagagctt ggcagtggcgctggcctcacaggcccggccatccgcaagatgtgtcgcccccaggcatacatcttcagcgactgt 15 cacagccgggtcctcgagcagctccgagggaatgtccttctcaatggcctctcattagaggcagacatcactgcc aecttagacgccccaggagaccacaggagaa in aacaaceactitcrtgggdcg in ggacagggcccttgagaetaaggt ggtgtttggctgggccaccgaaaaccccticacccctgccagcacactcagtcccctccctggtggaacagagctc tgcctgtggccctgggtcccagccctgaaacccacaggtccagcggtggccagggacacaggcccacccctgcaa gccagcagaccaaacggcagacacctgaaacaagaagttcacgacgtgctgtattgcccagaagccatcgtgtca 20 ctggtcggggtcctgcggaggctggctgcctgccgggagcacaagcaggctcctgaggtctacctggcctttacc gtccgcaacccagagacgtgcc agctgttcaccaccgagctagagatagcgtctttctgcaacctgcggtcccag cagaaaaaccttgtgatccttgttccagtcgacatggaggacgactcactctacttgggaggtgagtggcagttc aaccacttttcaaaactcacatcttctcggccagatgcagcttttgctgaaatccagcgtacttctctccctgag aagtcaccactctcatgtgagacccgtgtcgacctctgtgatgatttggctcctgtggcaagacagcttgctccc 25 agggagaagcctcctctgagtagcaggagacctgctgcggtgggggctgggctccagaatatgggaaatacctgc tacgtgaacgcttccctgcagtgcctgacatacaaaccgccacttgccaactacatgctgttccgggagcactct caaacgtgtcatcgtcacaagggctgcatgctctgtactatgcaagctcacatcacaagggccctccacattcct ggocatgtcatccagccctcacaggcatcggctgctggcttccatagaggcaagcaggaagatgcccatgaattt ctcatgttcactgtggatgccatgagaaaggcatgccttcccgggcacaagcaggtagatcgtcactctaaggac 30 accaccctcatccaccaaatatttggaggctactggagatctcaaatcaagtgtctccactgccacggcatttca gacacttttgacccttacctggacatcgccctggatatccaggcagctcacragtgtccagcaagcttitggaacag ttggtgaagcccgaagaactcaatggagagaatgcctatcattgtggtgtttgtctccagagggcgccggcctco aagacgttiaacttt this cacaactictgccaaggtcctcatccttgtattgaagagattccccgatgtcacaggcaac aaaattgccaagaatgtgca atatcctgagtgccttgacatgcagccatacatgtctcagcagaacacaggacct 35 ctcgtctatgtcctctatgctgtgctggtccacgctgggtggagttgtcacaacggacattactcctcttatgtc aaagctcaagaaggccagtggtataaaatggatigatgccgaggtcaccgcctctagcatcacttictgtcctgagt caacaggcctacgtccCcttttacatccagaagagtgaatgggaaagacacagtgagagtgtgtcaagacrgcagg gaacca ^ gagcccttggcgtagaagacacagacaggcgagcaacgcaagcragagctcaagagagaccacccctgc ctccaggcccccgagttggacgagcacttggtggaaagagccactcaggaaagcaccttagaccactggaaattc 40 cttcaagagcaaaacaaaacgaagcctgagttcaacgtcagaagagtcgaaggtacggtgcctcccgacgtactt gtgattcatcaatcaaaatacaagtgtcggatgaag & accatcatcctgaacagcaaagctccccgctaaacctc tcttcgacgaccccgacagatcaggagtccatgaacactggcacactcgcttccctacgagggaggaccaggaga tccaaagggaagaacaaacacagcaagagggctctgcttgtgtgccagtga 45 set bDUB4,1 & interpretation of the polypeptide sequence of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 505,560

MTLQQSMPFCIEHA MNHRRRNCESAYVSPRPDPTIPGTLDLTGPASRAPNPDSAPPKLRILGPAPGPASEDHGL APGPSSKPPQSRPGFLQVSRQRCGSAHVMAPEENAGTELWLQGFERRFLAARSLRSFPWQSLEAKLRDSSDSEUi rdilqktvkhpvcvkhppsvkyarcflselikkgaswtsstravhtepldelyevlaetlmakestqghrsyll PSGGSFTLSEITA work station working SHGTTGLVTWDATLYLAEWAIENPAAFTNRGVLELGSGAGLTGUaCKMCRPQAYIFSDC HSRVLEQLRGNVLUiGLSLEADITANLDAPGDHRRKTrTSGTRTGPLRKGGVWLGHRKPLTPASTLSPLSGGTEL CLWPWVPALKPTGPAVAf © TGPPLQASRPNGRHLKQEVHDVLYCPEAIVSLVGVLRRLAACREHKQAPEVYLAFT VRNPETCQLFTrELEXASFCNliRSiMKNLVILVPVDMEDDSt.YIiGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPE KSPLSCETRVDLCDDLAPVARQLAPREKPPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYKPPLAliYMLFREHS QTCHRHKGCMLCTMQAHITRAIiHIPGHVIQPSQALAAGFHKGKQEDAHEFLMFTVDAMRKACLPGHKQVDRHSKD τΤΕΙΗ〇ΙΡΟΟΥΐ «15〇ΙΪ« ΧΗ (: ΗΟΙ50ΤΗ) ΡΥυ > ENGINEERING ALDIQAAQSVQQALEQIiVKPEELNGENA'YHCGVCIiQRAPAS KTLTLHNSAKVLILVLKRFPDVTGNKIAKNVQYPECLDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYSSYV KAQEGQWYKMDDAEVTASSITSVLSQQAYVIiFYXQKSEWERHSESVSRGREPRALGVEDTDRRATQGEIiKRDHPC Ι ^ ΑΡΕΙ ^ ΕΗΙίνΕΚΑ ΟΕβΤΙΛΗΙΫΚΡΙ ^ ΕΟΝΚΤΚΡΕΡΝνΚΚνΕατνΡΡΟνίΛ / work HQSKYKCRMKNHHPEQQSSLLNL 2ESMNTGT

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This paper 3TLASLRGRTRRSKGKNKHSKRALLVCQ -65- applies China National Standard Scale (CNS) A4 size (210 X 297 mm) A7 B7 1312008 V. invention is described in (64) a nucleotide sequence atggaggacgactcactctact: dagger gggaggtgagcggcagttcaaccactcttcaaaactcacatcttctcggcca 5 gacgcagcttttgctgaaatccagcgtacttctctccctgagaagtcaccactctcatgtgagacccgtgtcgac ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaagcctcctctgagtagcaggagacct gctgcggtgggggctgggctccagaatatgggaaatacctgctiacgtgaacgcttccctgcagtgcctgacatac aaaccgccacttgccaactacatgctgttccgggagcactctcaaacgtgtcatcgtcacaagggctgcacgctc tgtactatgcaagctcacatcacaagggccctccacattcctggccatgtcatccagccctcacaggcattggct iO gctggcttccacagaggcaagcaggaagatgcccatgaacctctcatgttcactgtggatgccatgagaaaggca tgccttcccgggcacaagcaggtagatcgtcactictaaggacaccacccticatccaccaaacatttggaggctac Tggagatctcaaatcaagtgtctccactgccacggcatttcagacacttttgacccttacctggacatcgccctg gatatccaggcagctcagagtgfcccagcaagcttCggaacagCtggtgaagcccgaagaactcaatggagagaat gcccatcattgtggtgtttgtctccagagggcgccggcctccaagacgttaacttCacacaact ctgccaaggtc 15 ctcatccttgtattgaagagattccccgatgtcacaggcaacaaaattgccaagaaCgtgcaatatcctgagtgc cttgacatgcagccatacatgcctcagcagaacacaggacctctcgtctatgtcctctatgctgtgctggtccac gctgggtggagttgtcacaacggacattiacCcctcttatsrtcaaagctcaagaagrgccagtggtataaaatggat gatgccgaggccaccgcctctagcatcacttctgtcctgagtcaacaggcctacgtcctcttttacatccagaag agtgaatgggaaagacacagtgagagtgtgtcaagaggcagggaaccaagagcccttggcgtagaagacacagac 20 aggcgagcaacgcaaggagagc tcaagagagaccacccc tgcc tccaggcccccgagt tggacgagcact tggtg gaaagagccactcaggaaagcaccCtagaccaccggaaattccttcaagagcaaaacaaaacgaagcctgagttc aacgtcagaagagtcgaaggtacggtgcctcccgacgtacttgtgattcatcaatcaaaatacaagtgtcggatg aagaaccatcatcctgaacagcaaagctccctgctaaacctctcttcgacgaccccgacagatcaggagtccatg aacactggcacactcgcttccctacgagggaggaccaggagatccaaagggaagaacaaacacagcaagagggct 25 ctgcttgtgtgccagtga hOTBA. lb whoof polypeptide sequence MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVARQIAPREKPPLSSRRP 30 ΑΑνσΑΟΙ ^ ΝΜαΝΤΟΥνΝΑδΕΟζΧΤΥΚΡΡΙΛΝΥΜΙ ^ ΚΕΗβΟΤζΤ speech ϋΗΚααΜΙ ^ ΤΜΟΑΗΙΤΚΑωΐΡΟΗνίΟΡδΟΑΙΑ ΑαΡΗΜΚΟΕΟΑΗΕΡΙ ^ ΜΡΤνΒΑΜΚΚΑα ^ ΡΟΗΚΟνΒΗΜΚΟΤΤΙ ^ ΙΗΟΙΓσΟΥΝΙ ^ ΙΚΟΙιΗαίΟΐεΟΤΡϋΡΥΙ ^ ΙΑΙ ^ ΠΙΟΑΑζ ^ νΟςΑΙ ^ Εζ ^ νΚΡΕΕΧΝΟΕΝΑΥΗΟσνα ^ ΟΚΑΡΜΚΤΙ ^ ΗΝεΑΚνίαΐΛ ^ ΚΚΡΡΟνΤσΝΚΙΑΚΝνΟΥΡΕΟ

sewerhsesvsrgrepralgvedtdrratqgelkrdhpclqapeldehlveratqestldhwkflqeqnktkpef 35 ΝνΚΙΐνΕΟΤνΡΡΠνίΛ / ΙΗ ^ ΚΥΚαΟΙΚΝΗΗΡΕΟ ^ βΙΛΝΧ ^ ΤΤΡΤΟΟΕεΜΝΤΟΤυ ^ ΚσΚΤΚΚεκαΚΝΚΗεΚΚΑ LLVCQ nucleotide sequence of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 40 atgggaaatacctgctacgtgaacgcttccttgcagtgcctgacatacacaccgccccttgccaactacatgctg tcccgggagcactctcaaacgtgtcatcgtcacaagggctgcatgctctgtactatgcaagctcacatcacacgg gccctccacaatcctggccacgtcatccagccctcacaggcattggctgctggcttccatagaggcaagcaggM gatgcccatgaatttctcatgttcactgtggatgccatgaaaaaggcatgccttcccaggcacaagcaggtagat catcactctaaggacaccaccctcatccaccaaatatttggaggctactggagatctcaaatcaagtgtctccac 45 tgccacggcatttcagacacttttgacccttacctggacatcgccctggatatccaggcagctcagagtgtccag caagctttggaacagttggtgaagcccgaagaacCcaatggagagaatgcctatcattgtggtgtttgtctccag agggcgccggcctccaagacgttaactttacacacctctgccaaggtcctcatccttgtatkgaagagattctcc gatgtcacaggcaacaagattgccaagaatgtgcaatatcctgagtgccttgacatgcagccatacatgtctcag cagaacacaggacctctt gtctatgtcctctatgctgtgctggtccacgctgagtggagttgtcacaacggacat 50 tacttctcttatgtcaaagctcaagaaggccagtggtataaaacggatgatgccgaggtcaccgccgctagcatc acttctgtcctgagtcaacaggcctacgtcctctCttacatccagaagagtgaatgggaaLagacatagtgagagt gtgtcaagaggcagggaaccaagagcccttggcgcagaagacacagacaggcgagcaacgcaaggagagctcaag agagaccacccctgcctccaggcccccgagttggacgagcacttggtggaaagagccactcaggaaagcacctta gaccactggaaattccttcaagagcaaaacaaaacgaagcctgagttcaacgtcagaaaagCcaaaggtacagtg 55 atcaaagttgaccagccccagaggaaagctgcccagggcacaactcagggctccgtagaaccacagaatcttggg cgcaaccctgctcaagcacccaaatgtgcatacgaacagggtctccgtgtgacggaacaCgtccactttcggcag cactacaattttggcaccaaatgtgctaactgcaattccaccatacaatgcgtaactggaaatggaggcaacatc gccgatcctgaacgatcgatgcgagaatccaggatatgcacggcttattttggccttttcccactgaaacaaggg ccagtattaaaaatgcagaaaaaccttgtgatcctcgttccagtcgacatggaggacgactcactctactCggga -66- This paper scales applicable Chinese National Standard (CNS) A4 size (210 X 297 male Chu) 1,312,008 V. description of the invention (65) ggtgagtggcagtC.caaccacttttcaaaactcacai: cl: tctcggcccgatgcagcttt tgctgaaatccagcgg acttctctccctgagaagtcaccaccctcatgcgagacccgtgccgacccctgtgatgatttggctcccgtggca agacagcttgctcccagggagaagccticctccgagcagcaggagacctgctgcggtgggggccgggctccagaat atgggaaatacctgctacgcgaacgcctcctcgcagtgcctgacaCacacaacgccccttgccaactacatgctg tcccgggagcactctcaaacgtgtcatcgtcacaagggctgcatgctctgCactatgcaagctcacatcacacgg gccctccacaatcctggccacgtcatccagccctcacaggcattggccgctggcttccatagaggcaagcaggaa gatgcccatgaatttctcatgttcactgtggatgccatgaaaaaggcacgccttcccgggcacaagcaggtagat catcactctaaggacaccaccctcatccaccaaatacttggaggctactggagatctcaaatcaagtgtctccac tgccacggcatttcagacacttttgacccttacctggacatcgccctagatatccaggcagctcagagtgtccag caagctttggaacagttggtgaagcccgaagaactcaacggagagaatgcctatcattctggtgtttgcctccag agggcgccggcctccaagacgttaactttacacacccccgccaaggtcctcatccttgtattgaagagattctcc gatgtcacaggcaacaagactgccaagaatgtgcaatatcctgagtgccttgacatgcagccatacacgtctcag cagaacacaggacctcttgtctatgtcctctatgctgtgctggtccacgccgggtggagttgtcacaacggacat tacttccctcatgtcaaagctcaagaaggccagtggtaCaaaatggacgatgccgaggccaccgccgcca gcatc 15 20 acttctgccctgagtcaacaggcctacgtcctcttttacatccagaagagtgaaitgggaaagacacagtgagagt gtgtcaagaggcagggaaccaagagcccttggcacagaagacacagacaggcgagcaacgcaaggagagcCcaag agagaccacccctgccCccaggcccccgagttggacgagcacttggtggaaagagccactcaggaaagcacctta gaccactggaaattccttcaagagcaaaacaaaacgaagcctgagttcaacgccagaaaagtcgaaggtaccctg cctcccgacgtactcgtgattcatcaatcaaaatacaagtgtgggatgaagaaccaticatcctgaacagcaaagc tocctgctaaacctctcttcgtcgaccccgacacatcaggagtccatgaacactggcacactcgcCtcccCgcga gggagggccaggagatccaaagggaagaacaaacacagcaagagggctctgcttgtgtgccagtga hDUB4 · 2a interpretation polypeptide sequence valves

25 mgntcyvnaslqcltytpplanymlsrehsqtchrhkgcmlctmqahitralhnpghviqpsqalaagfhrgkqe DAHEFLMFTVDAMKKACLPRHKQVDHHSKDTTL station HQIFGGYWRSQIKCLHCHGISDTFDPYLDIALDIQAAQSVQ QALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPECLDMQPYMSQ QNTGPLVYVLYAVLVHAEWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASITSVLSQQAyVLFYIQKSEWERHSES DHWKFLQEQNKTKPEFNVRKVKGTV 30 35 VSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDF IKVDQPQRKAAQGTTQGSVEPQNLGRNPAQAPKCAYEQGLRVTEHVHFRQimiFGTKCANCNSTIQCVTGNGGNI ΑΟΡΕΪ ^ ΜΚΕβίΙΙζΤΤΑΥΡσί ^ ΡΙ ^ ΚΟσΡνί ^ ΚΜΟΚΝΙΛΠΧνΡνΟΜΕϋΟεί ^ ΙΛΟΕΜςΡΝΪ ^ ΚΙ / ^ ΚΡΟΑΑΡΑΕΙΟΐΙ TSLPEKSPLSCETRVDLCDDLAFVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVliASLQCLTYTTPLANYML δΚΕΗΒΟΤΟΗΗΗΚσΟΜΙΧΤΜΟΑΗΙΤΚΑ ^ ΗΝΡαΗνίΟΡβΟΑΙΑΑαΡΗΙΜΚΟΕΟΑΗΕΡΧΜΡτνΌΑΜΚΚΑίΧΡαΗΚΟνΟ HHSKmTLIHQIFGGYWRSQIKCXiHCHGlSDTroPYLDIALDIQAAQSVQQALEQLVKPEELNGENAYHSGVCLQ RAPASKTLTLHTSAKVIilLVLKRFSDVTGNKIAKNVQYPECLDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGH YFSYVKAQEGQWYKMDDAEVTAASITSALS () QAYVLFYIQKSEWERHSESVSRGREPRALGTEDTDRRATQGELK ΐωΗΡΙ ^ ΟΑΡΕωΕΗΙΛ / ΕΗΑΤΟΕδίΊ ^ ΟΗνίΚί'ΐΧΪΕΟΝΚΤΚΡΕΡΝνΚΚνΕσΤΙ ^ ΡΕΌνΐ ^ νΐΗΟεκνΚεΟΜΚΝΗΗΡΕΟΟε sllnlssstpthqesmntgtlaslrgrarrskgknkhskrallvcq member of the Intellectual Property Office of Economic Affairs H 40 nucleotide sequence consumer cooperatives printed atggaggacgactcactctacttgggaggtgagtggcagttcaaccacttttcaaaactcacatcttctcggccc gatgcagcttttgctgaaatccagcggacttctctccctgagaagtcaccactctcatgtgagacccgtgtcgac ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaagccCcctctgagtagcaggagacct 45 gctgcggtgggggctgggctccagaatatgggaaatacctgctacgtgaacgcttccttgcagcgcctgacatac acaacgccccttgccaactacatgctgtcccgggagcactctcaaacgtgtcatcgtcacaagggctgcatgctc tgtactatgcaagctcacatcacacgggccctccacaatcctggccacgtcatccagccctcacaggcattggct gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgttcactgtggatgccatgaaaaaggca tgccttcccgggcacaagcaggtagatcatcactctaaggacaccaccctcatccaccaaatatttggaggctac 50 tggagatctcaaatcaagtgtctccactgccacgg catttcagacacttttgacccttacctggacatcgcccta gatatccaggcagctcagagtgtccagcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat gcctatcattctggtgcttgtctccagagggcgccggcctccaagacgttaactttacacacctctgccaaggtc ctcatccttgtattgaagagaCtctccgatgtcacaggcaacaagattgccaagaaCgtgcaatatcctgagtgc cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccac 55 gcCgggtggagttgtcacaacggacattacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat gatgccgaggtcaccgccgctagcatcacttctgccctgagtcaacaggcctacgtcctcttttacatccagaag agcgaatgggaaagacacagtgagagtgtgtca «Lgaggcagggaaccaagagcccttggcacagaagacacagac aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggcccccgagttggacgagcacttggtg gaaagagccactcaggaaagcaccttagaccactggaaattccttcaagagcaaaacaaaacgaagcctgagttc 60 aacgtcagaaaagtcgaaggtaccctgcctcccgacgtacGtgtgattcatcaaCcaaaatacaagtgtgggatg ^ -67- This paper scales applicable Chinese National Standard (CNS) A4 size (210 X 297 mm) 1312008 A7 B7 V. invention is described in (66) aagaaccatcatcctgaacagcaaagctccctgctaaacctctcctcgtcgaccccgacacatcaggagtc catg aacactggcacacCcgct & ccctgcgagggagggccaggagatccaaagggaagaacaaacacagcaagagggct ctgcttgtgtgccagtga 5 hDUB4-2b interpretation polypeptide sequence ΜΕ005Ιι (ΥΙ ^ αΕΜζ3ΡΝΗΣ: · 5ΚΙ ^ Τ55ΗΡΟΑΑΡΑΕΙζ3ΚΤ5Ι ^ ΕΚ5ΡΙ ^ ΕΤίΐνϋΙΧ: ϋΟΙΛΡνΑΙΙ (2ΙΑΡΚΕΚυΡΙ ^ 5ί ^ Ρ aavgaglqnmgntcyvnaslqcltyttplanymlsrehsqtchrhkgcmlctmqahitralhnpghviqpsqala ΑσΡΗί ^ ΚΟΕΒΑΗΕΡυ ^ ΤνϋΑΜΚΚΑΟΧΡσΗΚΟνϋΗΗΞΚΟΤΤΙ ^ ΙΗΟΙΡσαΥνι / ί ^ ΙΚςΤΙ ^ ΗαΐΒΟΤΡϋΡΥΙ ^ ΙΑΙι SAKVLIIA; 10 15 20 25 30 35 ΒΙΟΑΑΟΗνΟΟΑΙνΕζ ^ νΚΡΕΕΧ ^ ΕΝΑΥΗΒΟνίΤΜΚΑΡΑεΚΤΙ ^ ΗΤεΑΚνίιΙΙΑ ^ ΚΚΓΒΟντσΝΚΙΑΚΝνΟΥΡΕΟ LDMQPYMS〇QNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASIT; SAIiSQQAYVLFYIQK SEWERHSESVSRGREPRALGTEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNKTKPEF NVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRA LLVCQ 1U3UB4.3 nucleotide sequence atggaggacgactcactctacttgggaggtgagtggcagttcaaccact tttcaaaactcacatcttctcggccc gatgcagcttttgctgaaatccagcggacttctctccctgagaagtcaccactctcatgtgagacccgtgtcgac ctctgtgatgatttggctcctgtggcaagacagcttgctcccagggagaagcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaatatgggaaatacctgccacgtgaacgcttccttgcagtgcctgacatac acaccgccccttgccaactacatgctgtcccgggagcactctcaaacgtgtcatcgtcacaagggctgcatgctc tgtacgatgcaagctcacatcacacgggccctccacaatcctggccacgtcatccagccctcacaggcattggct gctggcttccatagaggcaagcaggaagatgcccatgaatttctcatgttcactgtggatgccatgaaaaaggca tgccttcccgggcacaagcaggtagatcatcactctaaggacaccaccctcatccaccaaatatttggaggctac tggagatctcaaatcaagtgtctccactgccacggcatttcagacacttttgacccttacotggacatcgccctg gatacccaggcagctcagagtgtccagcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat gcccatcattgtggtgtttgtctccagagggcgccggcctccaagacgttaactttacacacctctgccaaggtc ctcatccttgtattgaagagattctccgatgtgacaggcaacaagattgccaagaatgtgcaatatcctgagtgc cfctgacafcgcagccatacatgtctcagcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccac gctgggtggagttgtcacaacggacattacttctcttatgtcaaagctcaagaaggcca atggtaCaaaatggat gatgccgaggtcaccgccgctagcatcacttctgtcctgagtcaacaggcctacgtcctcttttacatccagaag agtgaat9ggd.aagacacagtgagagtgtgtcaaga9gcagggaaccaagagcccttggcgcagaagacacagac aggcgagcaacgcaaggagagcCcaagagagaccacccctgcctccaggcccccgagttggacgagcacttggtg gaaagagccactcaggaaagcaccttagaccgcCggaaattccttcaagagcaaaacaaaacga ^ Lgcctgagttc aacgtcagaaaagtcgaaggtaccctgcctcccgacgtacttgtgattcatcaatcaaaatacaagtgtgggatg aagaaccatcatcctgaacagcaaagctccctgctaaacctctcttcgtcgaccccgacacatcaggagtccatg aacactggcacactcgcttccctgcgagggagggccaggagatccaaagggaagaacaaacacagcaagagggct ctgctcgtgtgccagtga hl3UB4-3 polypeptide sequence interpretation 45

ΜΕϋΕ ^ ΥΙ ^ ΟαΕϊίΟΠαί ^ ΚΙιΤ ^ ΚΙΌΑΑΡΑΕίΟΚΤεΐιΡΕΚβΡΙ ^ ΕΤΚνΟίιαυΓΪΙΑΡνΑΚΟΙΑΡΚΕΚυΡΙ ^ ΚΚΡ AAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRHKGCMLCTMQAHITRAI.HNPGHVIQPSQALA AGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQXFGGYWRSQIKCLHCHGISDTFDPYLDXAL DIQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVLXIiVLKRFSDVTGNKIAKNVQyPEC

MCGVCLQRft «SCHNGHYF Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 50

Ι ^ ΜΟΡΥΜεΟΟΝΤσΡΙινΥνίιΥΑνίινΗΑσ ^ ίΓΗΝσΗΥΡεΥνΚΑΟΕΟΟνηΓΚΜΟϋΑΕνΤΑΑΒΙΤενί ^ ΟΟΑΥνί ^ ΎΙΟΚ SEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDRWKFLQEQNKTKPEF NVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSIXNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRA LLVCQ ω〇Β4 2.5 nucleotide sequence atgcgccagagagctcgtcatttgaagactctctcggaagggatagcgtctttctgcaacctgcggtcccagcag 55 aaaaaccttgtgatccttgttccagtcgacatggaggaagactcactctacttgggaggtgagtggcagttcaac cacttttcaaaacrcacaccccctcggcccgatgcagcttttgctgaaatccagcggacttctctccctgagaag tcaccactctcatgtgagacccgtgtcgacctctgtgatgatttggctcctgtggcaagacagcttgctcccagg gagaagcttcctctgagtaacaggagacctgctgcggtgggggctgggctccagaatatgggaaatacctgctac gtgaacgcttccttgcagtgcctgacatacacaccgccccttgccaactacatgctgtcccgggagcactctcaa -68- This paper scales applicable Chinese National Standard (CNS) A4 size (210 X 297 mm) 1312008 A7 B7 V. INSTRUCTIONS (67) 10 15 acgcgccatcgtcacaagggctgcacgccctgcacgatgcaagctcacatcacacgggccctccacaatcctggc cacgtcatccagccctcacaggcattggctgctggcttccatagaggcaagcaggaagatgcccatgaatttctc atgttcaccgtggatgccatgaaaaaggcatgcccccccgggcacaagcaggtggatcatcactctaaggacacc accctcatccaccaaatatttggaggctactggagatctcaaatcaagtgtctccactgccacggcatt dagger cagac acttttgacccctacctggacatcgccctggatiaticcaggcagctcagagtgtccagcaagctttggaacagttg gtgaagcccgaagaactcaatggagagaatgcctatcattgtggtgtttgtctccagagggcgccggcctccaag acgttaactttacracacctctgccaaggtccticatccttgtattgaagaga Jen tctccgatgtcacaggcaacaag attgacaagaatgtgcaatatcctgagcgccttgacatgaagctatacatgtctcagacgaactcaggacctctc gtctatgtcccctatgctgtgctggtccacgctgggtggagttgtcacaacggacattacttctcttatgtcaaa gctcaagaaggccagtggcataaaacggatgatgccgaggtcaccgcctctagcatcacctctgtcctgagtcaa caggcctacgccctctcctacatccagaagagtgaatgggaaagacacagtgagagtgtgtcaagaggcagggaa ccaagagccctcggcgcagaagacacagacaggcgagcaacgcaaggagagctcaagagagaccacccctgcctc caggcccccgagttggacgagcacctggtggaaagagccactcaggaaagcaccttagaccactggaaattcctt caagagcaaaacaaaacgaagcccgagttcaacgtcagaaaagtcgaaggtaccctgcctcccgacgtacttgtg at Tcatcaatcaaaatacaagtgtgggatgaagaaccatcatcctgaacagcaaagctccctgctaaacctctct tcgacgaccccgacacatcaggagtccatgaacactggcacactcgcctccctgcgagggagggccaggagatcc aaagggaagaacaaacacagcaagagggccctgcttgtgtgccagtggtctcagtggaagtaccgacccaca 20 25

hOTB4-5 deductive polypeptide sequence εΡΙ^ΕΤΚνΟίΤΟΟΙ^ΑΡνΑΚΟΙΛΡΙΙΕΚΧ^ΡΙ^ΝΗΚΡΑΑνσΜΙ^ΝΜσΝΤΟΥνΝΑΒΙ^Οα^ΤΥτΡΡΙΑΝΥΜΙ^ΗΕΗβ. TCHRHKGCMLCTMQAHITRALHNPGHVIQPSQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDT ΤΙίΙΗ〇ΙΓΟΟΥϊίΙ13ζ3Ι:Κ(:ΓιΗζ:Η(;ΐ5〇ΤΡΌΡ'ϊΧΕ)ΙΑΓ^Ι〇ΑΑ<35ν〇〇ΑΙίΕ〇Ι<1νΚΡΕΕΙΛΝ(5ΕΝΑΥϊΚ:σν(:Ι*〇Ι^ΑΡΑ3Κ ΊΧΊΧΗΤΒΑΚν^ΙΙΛ^Κί ^ ΟνΤΟΝΚΊΏΚΝνί ^ ΥΡΕα ^ ΜίαΥΜδΟΤΝεσΡΙ ^ νΥνί ^ Αν ^ νΗΑΟνίεαΗΝσΗΥΡεΥνΚ AQEGQWYKMDDAEVTASSITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCL QAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEOQSSLLNLS STTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQWSQWKYKPT 30 hBUB4 «$ 354,045 nucleotide sequence 醆 Ministry of economic Affairs intellectual property Office employees consumer cooperatives printed 50 55 60 atggaggacg acatcttctc tcaccactct cagcttgctc gggctccaga acaccgcccc aagggctgta cacgtcatcc gcccatgaat aagcaggtgg tggagatctc Ctggacatcg gtgaagcccg gcgccggcct aagagattct cttgacatgc gctgtgctgg gctcaagaag tctgtcctga cacacrtgaga aggcgagcaa gacgagcac t caagagcaaa cccgacgtac gaacagcaaa aacactggca cacagcaaga actcactcta ggcccgatgc catgtgagac ccaggcragaa atatgggaaa ctgccaacta Cgctctgtac agccctcaca ttctcatgtt atcatcactc aaatcaagtg ccctggacat aagaactcaa ccaagacgtt ccgatgtcac agccatacat tccacgctgg gccagtggta gtcaacaggc gtgtgtcaag cgcaaggaga tggtggaaag acaaaacgaa ttgtgattca gctccctgct cactcgcttc gggctctgct cttgggaggt agcttttgct ccgtgtcgac gcttcctctg tacctgctac catgctgtcc gatgcaagct ggcattggct cactgtggat taaggacacc tctccactgc ccaggcagct cggagagaat aactttacac aggcaac ^ ag gtctcagacg gcggagttgt taaaatggat ctacgtcctc aggcagggaa gctcaagaga agccactcag gcctgagttc tcaatcaaaa aaacctctct cctgcgaggg tgftgcgccag gagtggcagt gaaatccagc ctccgtgatg agtagcagga gtgaacgctt cgggagcact cacatcacac gctggcttcc gccatgaaaa accctcatcc cacggcattt cagagtgtcc gcctatcatt acctctgcca attgccaaga aacacaggac cacaacggac gatgccgagg ttttacatcc ccaagagccc gaccacccct gaaagcacct aacgtcagaa tacaagtgtg tcgacgaccc agggccagga tga tcaaccactt ggacttctct atttggctcc gacctgctgc ccttgcagtg ctcaaacgtg gggccctcca acagaggcaa aggcatgcct acca aatatt cagacacttt agcaagcttt gtggtgtttg aggtcctcat atgtgcaata ctctcgtcta attacttctc tcaccgcctc agaagagtga ttggcgcaga gcctccaggc tagaccactg aagtcgaagg ggatgaagaa cgacacatca gatccaaagg ttcaaaactc ccctgagaag tgtggcaaga ggtgggggct cctgacatac tcatcgtcac caatcctggc gcaggaagat tcccgggcac tggaggctac tgacccttac ggaacagttg tctccagagg ccttgtattg tcctgagtgc tgtcctctat ttatgtcaaa tagcatcact atgggaaaga agacacagac ccccgagttg gaaattcctt taccctgcct ccatcatcct ggagtccatg gaagaacaaa hDUB4.6 polypeptide sequence interpretation • 69- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 V. Invention description (68)

MEDDSLYLGG EWQFNHFSKL TSSRPDAAFA EIQRTSLPEK SPLSCETOVD LCDDLAPVAR QLAPREKLPL SSRRPAAVGA GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED AHEFLMFTVD AMKKACLPGH 5 KQVDHHSKDT TLIHQIFGGY WRSQXKCLHC HGXSDTFDPY LDXALDIQAA QSVQQALEQL VKPEELNGEN AYHCGVCLQR APASKTLTLH TSAKVLILVL · KRFSDVTGNK IAKNVQYPEC LDMOPYHSOT NTGPLVYVLY AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTASS work T SVLSQQAYVL FYIQKSEWER HSESVSRGRE PRALGAEDTD RRATQGELKR DHPCLQAPEL DEHLVERATQ ESTLDHWKFL QEQNKTKPEF NVRKVEGTLP PDVLVXHQSK YKCGMKNHHP 10 EQQSSLLNLS STTPTHQESM NTGTLASLRG RARRSKGKNK HSKRALLVCQ hDDB4,7 nucleotide sequence atggaggacg actcactcta cttgggtggt gagtggcagt tcaaccactt ttcaaaactc 15 acaccttctc ggcccgatgc agcttttgct gaaatccagc ggacttctct ccctgagaag tcaccactct catgtgagac ccgtgtcgac ctctgtgatg acttggctcc tgtggcaaga cagcttgctc ccagggaqaa gcttcctctg agtagcagga gacctgctgc ggtgg ^ ggct gggctccaga atatgggaaa tacctgctac gtgaacgctt ccttgcagtg cctgacatac acaccgcccc ttgccaacta Catgctgtcc cgggagcact ctcaaacg tg tcatcgtcac 20 aagggctgca tgctctgtac tatgcaagct cacatcacac gggccctcca caatcctggc cacgccatcc agccctcaca ggcattggct gctggcttcc atagaggcaa gcaggaagat gcccatgaat ttctcatgtt cactgtggat gccatgaaaa aggcatgcct tcccgggcac aagcaggtag atcatcactc caaggacacc accctcatcc accaaatatt cggaggctac tggagatctc aaatcaactg tctccactgc cacggcattt cagacacttt tgacccttac 25 ctggacatcg ccctggatat ccaggcagct cagagtgtcc agcaagcttt ggaacagtCg gtgaagcccg aagaactcaa tggagagaat gcctatcatt gtggtgtttg tctccagagg gcgccggcct ccaagacgtt aactttacac acctctgcca aggtcctcat ccttgtattg aagagattct ccgatgtcac aggcaacaag attgccaaga atgtgcaata tcccgagtgc cttgacatgc agccatacat gtctcagcag e ^ cacaggac ctcttgtcta tgtcctctat 30 gctgtgctgg tccacgctgg gtggagttgt cacaacggac attacttctc ttacgtcaaa gctcaagaag gccagtggta taaaatgaat gatgccgagg tcaccgccgc tagcatcact tctgtcctga gtcaacaggc ctacgtcctc tttCacatcc agaagagtga atgggaaaga cacagtgaga gtgtgtcaag aggcagggaa ccaagagccc ttggcgcaga agacacagac aggcgagcaa cgrcaaggaga gctcaagaga gaccacccct gcctccag gc ccccgagttg 35 gacgagcact cggtggaaag agccactcag gaaagcacct tagaccact? gaaattcctt caagagcaaa acaaaacgaa gcctgagttc aacgtcagaa aagtcgaagg taccctgcct cccgacgtac ttgtgactca tcaatcaaaa tacaagtgtg ggatgaagaa ccatcatcct gaacagcaaa gctccctgct aaaccCctct tcgtcgaccc cgacacatca ggaggccatg aacactggca cactcgcCtc cctgcgaggg aggaccagga gatccaaagg gaagaacaaa 40 cacagcaaga gggctctgct tgtgtgccag tga ωϋΒ4 · 7 interpretation of the polypeptide sequence of Economic Affairs Intellectual Property Office Printed by employee consumption cooperatives

MEDDSLYLCG EWQFNHFSKL TSSRPDAAFA EXQRTSLPEK SPLSCETRVD LCDDLAPVAR 45 QLAPREKLPL SSRRPAAVGA GLQNMGNTCY VNASLQCIiTY TPPLANYMLS REHSQTCHRH KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED AHEFX * MFTVD AMKKACLPGH KQVDHHSKDT TLIHQIFGGY WRSQINCLHC HGISDTFDPY LDIALDXQAA QSVQQALEQL VKPEELNGEM AYHCGVCLQR APASKTLTLH TSAKVLILVI, KRFSDVTGNK IAKNVQYPEC LDMOPYHSOQ NTGPLVYVLY AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTAASIT 50 SVLSQQAYVL FYIQKSEWER HSESVSRGRE PRALGAEDTD RRATQGELKR DHPCLQAPEL DEHLVERATQ BSTXDHMKFL QEQNKTKPEF NVRKVEGTLP PDVLVXHQSK YKCOiKNHHP EQQSSLLNLS SSTPTHQEAH NTGTLASLRG RTRRSKGKNK HSKRALLVCQ hD0B4e8 nucleotide sequence 55 atgcgccaga gagctcgtca tttgaagact ctctcggaag ggatagcgtc ttgctgcaaa ctgcggtccc agcagaaaaa ccttgtgatc cctgttccag tcgacatgga ggacgactca ctctacttgg gaggtgagtg gcagttcaac cacttttcaa aactcacatc ttotcggccc gatgcagctt ttgctgaaat ccagcggact tctctccctg agaagtcacc actctcatgt 60 gagacccgtg tcgacctctg tgatgatttg gctcctgtgg caag & cagct tgctcccagg -70- This paper scale applies to Chinese national standards ( CNS) A4 size (210 X 297 mm) 1312008 A7 B7 V. invention is described in (69) 10 15 20 25 gagaagcttc ctcCgagtag caggagacct gctgcggcgg gggctgggcc ccagaatacg ggaaatacct gctacgtgaa cgcttccttg cagtgcctga catacacacc gcccctcgcc aaccacatgc tgtcccggga gcaccctcaa acgtgtcatc gtcacaaggg ctgcatgctc tgtacgatgc aagcccacat cacacgggcc ctccacaatc ctggccacgt catccagccc ccacaggcac cggctgctgg ctcccataga ggcaagcagg aagatgccca tgaacttccc atgttcactg tggatgccat gaaaaaggca tgccttcccg ggcacaagca ggtagatcaC cactctaagg acaccacccc catccaccaa atatttggag gctactggag atctcaaatc aagcgtctcc actgccacgg catttcagac acttttgacc ctcacctgga catcgccctg gatacccagg cagctcagag tgtccagcaa gctttggaac agttggtgaa gcccgaagaa cccaacggag agaatgccca tcattgtggt gtttgtctcc agagggcgcc ggcctccaag acgtcaactc tacacacctc tgccajaggtc ctcatccttg tattgaagag attccccgat gcgacaggca acaagattgc caagaatgtg caatatcctg agtgccttga catgcagcca tacatgtctc agcagaacac aggacccctc Gtctatgtcc tctatgcCgt gctggtccac gctgggtgga gttgtcacaa cggacaCtac ttctcttatg tcaaagctca a gaaggccaa tggtataaaa tggatgatgc cgaggtcacc gccgccagca tcacttctgt cctgagtcaa caggcctacg tcctctttta catccagaag agtgaatggg aaagacacag tgagagtgtg ccaagaggca gggaaccaa? agcccttggc gcagaagaca cagacaggcg agcaacgcaa ggagagctca agagagacca cccctgcctc caggcccccg agtcggacga gcacttggtg gaaagagcca ctcaggaaag caccttagac cactggaaat tccttcaaga gcaaaacaaa acgaagcctg agttcaacgt cagaaaagtc gaaggtaccc tgcctcccga cgtacttgtg actcatcaat caaaatacaa gtgtgggatg aagaaccatc atcctgaaca gcaaagctcc ctgctaaacc tctcttcgtc gaccccgaca catcaggagt ccatgaacac tggcacactc Gcttccctgc gagggagggc caggagaccc aaagggaaga acaaacacag caagagggct ctgcttgtgc gccagcga hDUB4.8 deductive peptide sequence

MRQRARHLKT LSEGIASCCK LRSQQKNLVI LVPVDMEDDS LYLGGEWQFN HFSKLTSSRP DAAFAEXQRT SLPEKSPLSC ETRVDLCDDL APVARQIAPR EKLPLSSRRP AAVGAGLQNM 30 GNTCYVNASL QCLTYTPPLA NYMLSHEHSQ TCHRHKGCML CTMQAHITRA LHNPGHVIQP SQALAAGFHR GKQEDAHEFL MFTVDAMKKA CLPGHKQVDH HSKDTTLIHQ IPGGYWRSQ station KCLHCHGISD TFDPYLDIAL DIQAAQSVQQ ALEQLVKPEE LNGENAYHCG VCLQRAPASK TLTLHTSAKV LILVLKRPSD VTGNKIAKNV QYPECLDMQP YMSQWTGPL VYVLYAVLVH AGWSCHNGHY FSYVKAQEGQ WYKMDDAEVT AASITSVLSQ QAYVLPYXQK SEWERHSESV 35 SRGREPRALG AEDTDRRATQ GELKRDHPCL QAPELDEHXiV ERATQESTLD HWKFLQEQNK TKPEFNVRKV EGTLPPDVLV IHQSKYKCGM KNHHPEQQSS LLNLSSSTPT HQESMNTGTL ASLRGRARRS KGKNKHSKRA LLVCQ 40 nucleotide sequence atgtgcatac gaacagggtc actggcagac agcttatcga ccgatgccca gggaacatgt aactgcaatt ccaccataca booked 45 Ministry of economic Affairs intellectual property Office employees consumer cooperatives printed 50 55 60 cgaccgatgc gggccagtat aggctatcgc ctcaggtcag gctgccaaaa cccgttccag gagaatccag taaaaatggt Cggaagggaa aaagcgcact ggatagcgtc tcgacatgga Cacttttcaa aactcacatc tctctccctg agaagtcacc gctcctgtgg caagacag ct gctgcggtgg gggctgggct cagtgcctga catacacaac acgtgtcatc gtcacaaggg ctccacaatc ctggccacgt ggcaagcagg aagacgccca tgccttcccg ggcacaagca atatttggag gctactggag tccgtgtgac ttgggctccc ccactttcgg atgcgtaact gatatgcacg aatttcactc gaaaatacgc ttcgacttct tttctgcaac ggacgactca ttctcggccc actctcatgt tgctcccagg ccagaatatggccccttgcc ctgcatgctccatccagccc tgaatttctc ggtagatcat atctcaaatc gtgtgtgaaa ctcaaaatcg cagcattaca ggaaatggag gcttattttg ggacagagaa tgtgctaaat tgtccttccg ctgcggtccc ctctacttgg gatgcagctt gagacccgtg gagaagcttc ggaaatacct aactacatgc tgtactatgc tcacaggcat atgttcactg cactctaagg aagtgtctcc actacagtgt gttatgagca attttggcac gcaacatcgc gccttttccc tcaataggct actatacttc tcgctgagag agcagaaaaa gaggtgagtg ttgctgaaat tcgacctctg ctctgagtag gctacgtgaatgtcccggga aagctcacat tggctgctgg tggatgccat acaccaccct actgccacgg gatgagcatg ttcaagcaca caaatgtgct cgatcctgaa ectgaaacaa caacgtggaa attgactatt gatgatggca ccttgtgatc gcagttcaac ccagcggact tgatgatttg caggagacct cgcttccttg gcactctcaa cacacgggcc cttccataga Gaaaaaggca catccaccaa catttcagac -71- This paper size applies to China National Standard (CNS) A4 specification (210x297 mm) 1312008 A7 B7 V. Invention description (7〇actcttgacc gctttggaac gtccgtctcc ctcatcctcg 5 caatatcctg gtctatgtcc tcctcttatg gccgctagca agtgaatggg 10 acagaagaca caggcccccg cactggaaat gaaggtaccc aagaaccatc 15 catcaggagt aaagggaaga cctacctgga agttggtgaa agagggcgcc tattgaagag agtgccttga tctatgctgt ccaaagctca Ccacttctgc aaagacacag cagacaggcg agttggacga tcctccaaga tgcctcccga atcccgaaca ccatgaacac acaaacacag catcgcccta gcccgaagaa ggcctccaag accccccgac cacgcagcca gccggtccac agaaggccag cctgagtcaa tgagagtgtg agcaacgcaa gcacctggtg gcaaaacaaa cgtacttgtg gcaaagcCcc tggcacactc caagagggct gatatccagg ctcaatggag acgctsieictt: gtcacaggca cacatgcctc gccgggtgga cggtacaaaa caggcctacg tcaagaggca ggagagctca gaaagagcca acgaagcctg attcatcaat ccgccaaacc gcttccctgc Ctgcttgtgt cagctcagag agaacgccta cacacacctc acaagatcgc agcagaacac gttgtcacaa tggacgatgc tcctctttta gggaaccaag agagagacca ctcaggaaag agttcaacgt ca aaatacaa tccccccgtc gagggagggc gccagtga tgtccagcaa Ccattctggt tgccaaggtc caagaatgtg aggacctctt cggacattac cgaggtcacc cacccagaag agcccttggc cccctgcctc caccttagac cagaaaagtc gtgtgggatg gaccccgaca caggagatcc 20 25 30 hDOB4 · 10 play whoof polypeptide sequence MCXRTGSPCD VCENYSVMSM NCNSTIQCVT GNGGNIADPE RLSLEGKKIR CAKYYTStiTI LVPVDMEDDS LYLGGEWQFN APVARQLAPR EKLPLSSRRP TCHHHKGCML CTMQAHXTRA CLPGHKQVDH HSKDTTLXHQ ALEQLVKPEE LNGENAYHSG QYPECLDMQP YMSQQNTGPL TSALSQ QAYVLFYIQK ^ DEHLV ERATQEi KNHHPEQQSS LLNLSS; AASIT: QAPEL1

ESTLD SSTPT

TGRQLIDWAP RSMRESRICT bRSESALSTS HFSKLTSSRP AAVGAGMNM LHNPGHVXQP IFGGWRSQI VCLQRAPASK VYVLYAVLVH SEWERHSESV HWKFLQEQNK HQESMNTGTL

LK work GYEHSST AYFGLFPLKQ CPSVAERMMA DAAFAEIQRT GNTCYVNASL SQALAAGFHR KCLHCHGISD TLTLHTSAKV AGWSCHNGHY SRGREPRALG TKPEFNVRKV ASLRGRARRS

PMPREHVHFR GPVLKMVISL AAKRIASFCN SLPEKSPLSC QCLTYTTPLA GKQEDAHEFL TFDPYLDIAL LILVLKRFSD FSYVKAQEGQ TEDTDRRATQ EGTLPPDVLV KGKNKHSICRA

QHYNFGTKCA GQRINRLNVE LRSQQKNLVI ETRVDLCDDL .SREHSQ rDAMKKA DIQAAQSVQQ VTGNKIAKNV WYKMDDAEVT GELKRDHPCL IHQSKYKCGM LLVCQ booked 354,045 Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 50 55 60 hDOBUl nucleotide sequence atgtgcatac gaacagggtc tccgtgtigac gtgtgtgaaa actacagtgt gatgagcatg actggcagac agcttatcga ttgggccccc ctcaaaatcg gttatgagca ttcaagcaca ccgatgccca ggacacttta catccggcac aggaagcctt ctgatggagc acacctggcc catgaaaaga caagggaaag aaacggggcc aaagggaaga aaatacgctg tgctaaatac tatacttcac tgactattct caggtcagaa agcgcacttt cgtcttcttg tccttccgtc gcggagagga tgatggcagc tgccaaaatc gacatggagg acgactcact ctacttggga ggtgagtggc agttcaacca cttttcaaaa ctcacatctt ctcggccaga tgcagctttt gctgaaaccc agcggacttc tctccctgag aagtcaccac tctcatatga cttggctcct gtggcaagac agcctgctcc cagggagaag cttcctctga gtagcaggag acctgctgcg gtgggggctg ggctccagaa tatgggaaat acctgctacg tgaacgcttc cttgcagtgc ctgacataca caccgcccct tgccaactac atgctgtccc gggagcactc Ccaaacgtgt catcgtcaca agggctgcat gctctgtact atgcaagctc acatcacacg g gccctccac aatcctggcc acgtcatcca gccctcacag gcattggctg ctggcttcca tagaggcaag caggaagatg cccatgaatt tctcatgttc actgtggatg ccatgaaaaa ggcatgcctt cccaggcaca agcaggtaga tcatcactct aaggacacca ccctcatcca ccaaatattt ggaggctact ggagatctca aatcaagtgt ctccactgcc acggcatttc agacactttt gacccttacc tggacatcgc cctggatatc caggcagctc agagtgtcca gcaagctttg gaacagttgg tgaagcccga agaactcaat ggagagaatg cctatcattg tggtgtttgt ctccagaggg cgccggcctc caagacgtta actttacaca cctctgccaa ggtcctcatc cttgtattga agagattctc cgatgtcaca ggcaacaaga ttgccaagaa tgtgcaatat cctgagtgcc ttgacatgca gccatacatg tctcagcaga acacaggacc tcttgtctat gtcctctatg ctgtgctggt ccacgctgag tggagttgtc acaacggaca ttacttctct tacgtcaaag ctcaagaagg ccagtggtat aaaatggatg atgccgaggt caccgccgct agcatcactt ctgtcctgag tcaacaggcc tacgtcctct tttacatcca gaagagtgaa tgggaaagac atagtgagag tgtgtcaaga ggcag ^ gaac caagagccct tggcgcagaa -72- this paper scales applicable Chinese national standard (CNS) A4 size (210 X 297 MM)

I 1312008 A7B7 V. invention is described in (71 gacacagaca cccgagttgg aaattccttc acccCgcctc caccatcctg gagtccatga aagaacaaac ggcgagcaac acgagcactt aagagcaaaa ccgacgtact aacagcaaag acactggcac acagcaagag gcaaggagag ggtggaaaga caaaacgaag cgcgattcat ctccctgcta actcgcttcc ggctctgctt ctcaagagag gccactcagg cctgagttca caatcaaaat aacctctctt ctgcgaggga gtgtgccagc accacccctg aaagcacctt acgtcagaaa acaagtgtgg cgtcgacccc gggccaggag ga cctccaggcc agaccactgg agtcaaaggt gatgaagaac gacacatcag atccaaaggg 10 15 20

hDUB4 · 11 deduced polypeptide sequence MCIRTGSPCD VCENYSVMSM HEKTRERNGA KGKKIRCAKY GEWQFNHFSK LTSSRPDAAF VGAGLQNMGN TCYVNASLQC NPGHVIQPSQ ALAAGFHRGK GGYWRSQIKC LHCHGISDTF ^QRAPASKTL· TLHTSAKVLI ifAVLVHAE WSCKNGHYF 3ESVSR GREPRALGA KFLQEQKKTK PEFNVRKVKG ESMNTGTLAS LRGRARRSKG

VLYAVI WERH5E

TGRQLIDWAP YTSLTiLRSE AEXQRTSLPE LTYTPPLANY Q£DAKEFLMF DPYLDIALDI LVLKRFSDVT S YVKAQEGQWY E DTDRRATQGE TLPPDVLVIH KNKHSKRALL

LKIGYEKSST SALSSSCPSV KSPLSYDLAP MLSREHSQTC TVDAMKKACL QAAQSVQQAL· GNKIAKNVQY KMDDAEVTAA LKRDHPCLQA QSKYKCGMKN VCQ

PMPRTLYIRH RKPSDGAHLA AERMMAAAKI DMEDDSLYLG VARQLAPREK LPLSSRRPAA HRHKGCMLCT MQAHITRALH PRHKQVDHHS KDTTLIHQIF EQLVKPEELN GENAYHCGVC PECLDMQPYM SQQNTGPLVY SXTSVLSQQA YVLFYXQKSE PELDEHLVER ATQESTLDHW HHPEQQSSLL NLSSSTPTHQ bDQB8 * l nucleotide sequence loading 2530354045 Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 50 55 60 atgggggacgactcactctactrgggaggtgagtggcagttcaaccacttttcaaaactcacaccttctcggcca gatgcagcttctgctgaaatccagcggacttctctccctgagaagtcaccactctcatctgagacccgtgtcgac ctctgtgatgatttggctcctgtggcaagacagctcgctcccagggagaagcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaatatgcrgaaatacctgctacgagaacgcttccctgcagCgcctgacatac acactgccccttgccaactacatgctgtcccgggagcactctoaaacatgtcagcgtcccaagtgctgcatgctc tgtactatgcaagctcacatcacatgggccctccacagtcctggccatgtcatccagccctcacaggcattggct gctggcttccatagaggcaagcaggaagatgtccatgaatttctcatgttcactgtggatgccatgaaaaaggca tgccttcccggccacaagcaggtagatcatcactgcaaggacaccaccctcatccaccaaatatttggaggctgc tggagatctcaaatcaagtgtctccactgccacgggatttcagacacttttgacccttacctggacat cgccctg gatatccaggcagctcagcLgtgtcaagcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat gcctatcattgcggtctttgtctccagagggcgccggcctccaacacgttaactttacacacttctgccaaggtc ctcatccttgtcttgaagagattctccgatgtcgcaggcaacaaacttgccaagaatgtgcaatatcctgagtgc cttgacatgcagccatacatgtctcagcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccac gctgggtggagttgtcacgacggacattacttctcctatgtcaaagctcaagaagtccagtggtataaaatggat gratgccgaggtcactgtctgtagcatcatttctgtcctgagtcaacaggcctatgtcctcttttacatccagaag acrtgaatgggaaagacacagtgagagtgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacagac aggcgagcaaagcaaggagagctcaagagagaccacccctgcctccaggcacccgagctggacgagcacttggtg gaaagagccactcaggaaagcaccttagaccactggaaattcctgcaagagcaaaacaaaacgaagcctgagttc aacgtcggaaaagtcgaaggtaccctgcctcccaacgcacttgtgattcatcaatcaaaatacaagtgtgggatg aaaaaccatcatcctgaacagcaaagctccctgctaaacctctcttcgacgacccggacagatcaggagtccatg aacactggcacactcgctitctctgcaagggaggacca ^ gagagccaaagggaagaacaaacacagcaagagggct ctgcttgtgtgccagtga hDDB8-l Yi Bin polypeptide sequence MGDDSLYLGGEWQFNHFSKLTSSRPDAAFAE IQRTSLPEKSPLSSETRVDLCDDLAPVARQLAPREKLPLSSRRP AAVGAGIiQNMGNTCYENASLQCLTYTLPLANYMLSREHSQTCQRPKCCMLCTMQAHITWALHSPGHVXQPSQALA AGFHRGKQEDVHEFLMFTVDAMKKACLPGHKQVDHHCKDTTLIHQIFGGCWRSQIKCLHCHGISDTFDPYLDIAL ΡΙΟΑΑΟΒνΚΟΑΙιΕΟΙίνΚΡΕΕΙιΝσΕΝΑΥΗαΜΧΙ^ΚΑΡΑΒΝΤΙ/ΠιΗΤΒΑΚνίιΙΙΛ/ΙιΚΚΡΒΟνΑΟΝΚΙΑΚΝνΟΥΡΕ. ldmqpymsqqntgplvyvlyavlvhagwschdghypsyvkaqevqwykmddaevtvcsiisvlsqqayvlfyiqk SEWERHSESVSRGREPRALGAEDTDRRAKQGELKRDHPCLQAPEliDEHLVERATQESTLDHWKFLQEQNKTKPEF NVGKVEGTLPPNALVIHQSKYKCGMKNHHPBQQSSLLNLSSTTRTOQESMNTGTLASLQGRTRRAKGKNKHSKRA hbVCQ HDUB8.3 -73- nucleotide sequence of the present paper is suitable China National Standard Scale (CNS) A4 size (210 X 297 mm) Order line A7 B7 1312008 V. invention is described in (72)

Atggaggacgactcactctacttgggaggtgagtggcagttcaaccact dagger ttcaaaactcacatcttctcggcca gaugcagccttcgctgaaatccagcGgacttctctccctgagaagtcacaactctcaactgagacccgcgtcgac ctctgcgatgacttggcgcctgtggcaagacagcttgctcccagggagaaGcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaatatgggaaatacctgctacgtgaacgcttcccagcagtgtctgacatac 5 Acaccgccccttgccaactacatgctgtcccgggagcactctcaaacatgtcatcgtcacaagtgctgcatgctc cgtaccatggaagctcacatcacatGgcccctccacattcctggccatgtcatccagccctcacaggcattggct gctggcttccatagaggcaagcaggaagctgcccttgaatttctcatgttCactgtggatgccatgaaaaaggca tgccttcccgggcacaagcaggtagatcatcactccaaggacaccaccctcatccaccaaatatttggagggtac Tggagatctcaaatcaagtgtctccactgccacggcatttcagacactttcggcccttacctggacatcgccctg 10 gatatccaggaagctcagagtgtcaAgcaagctttggaacagttggtgaagcccgaagaacccaatggagagaat gcctatcattgtggcaacaaaattgccaagaatgtgcaatatcctgagtgCcttgacatgcagccatacatgtct cagcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccacgccgggtggagttgtcacaacgga Cat tac t tc tc t Catgtcaaagt tcaagaaggccagtggtataaaatggatgatgccgaggtcactgcctctgg c atcacctctgccctgagtcaacag ^ ccatgtcctcttttacacccacaagagtgaatgggaaagacacagtgag 15 agtgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacagaCaggcgagcaacgcaaggagagctc aagagagactacccctgcctccaggtacccgagttggacgagcacttggtggaaagagocactcaggaaagcacc Ttagaccactggaaattcctccaagagcaaaacaaaacgaagcctgagttcaacgtcagaaaacttgaaggtacc ctgcctcccaacgtacttgtgatitcAtcaatcaaaatacaagtgtgggatgaaaaaccatcatcctgaacagcaa agctccctgctaaacctctcttcgacgaacccgacagatcaggagtccatGaacactggcacactcgcttctctg 20 caagggaggaccaggagagccaaagggaagaacaaacactgcaagagggctctgcttgtgtgocagtga Η〇〇Ββ 3 · 25 polypeptide sequence interpretation

3LSTET3 ZmKKO CMLCTMEAHITWPLHI GGYWRi

PGHVIQPSQ; SDTFGPYLD: VI.VHAGWSC

;QALA IAL 30

aavgaglqnmgntcywasqqcltytpplanymlsrehsqtci ΑΟΡΗΗσΚΟΕΑΑΙ ^ ΕΡΙιΜΓΓνϋΑΜΚΚΑα ^ ΗΚΟνΟΗΗεΚΟΤΤΙ ^ ΙΗΟΙΡσΟΥϊίΗβΟΙΚαιΗα ^ Ι ^ DXQEAQSVKQALEQLVKPEELNGENAYHCGNKIAKNVQYPECLDMQPYMSQQNPrGPLVYVLYAW HYFSYVKVQEGQWYKMDDAEVTASGITSVLSQQAYVLFYXHKSEWERHSESVSRGREPRALGAEDTDRRATQGEL KRDYPCLQVPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKLEGTLPPNVLVIHQSKyKCQlKNHHPEQQ SSLLNLSSTNPTDQESMNTGTIASLQGRTRRAKGKNXHCK31ALLVCQ booked 354,045 Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 50 55 60 ΗϋϋΒ8 · 5 nucleotide sequence Atggaggacgactcactctacttgggaggtgagtggcagttcaaccacttttcaaaactcacatcttctcggcca gatgcagcttttgctgaaacccagcGgacttctctccctgagaagtcaccactctcatctgag ^ cccgtgtcgac ctctgtgatgatttggctcctgtggcaagacagcttgctcccaggaagaaGcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaatatgggaaatacctgctacgagaacgcttccctgcagtgcctgacatac Acaccgccccttgccaactacatgctgtcccgggagcaccctcaaacatgtcagcgtcccaagtgctgcatgctc tgtactatgcaagctcacatcacatGggccctccacagtcctggtcatgtcatccagccctcacaggcattggct gctggc ttccatagaggcaagcaggaagatgcccatgaatttctcatgttCactgtggatgccatgaaaaaggca tgccttcccggccacaagcaggtagatcatcactctaaggacaccficcctcaticcaccaaatatttggaggctgc Tggagatctcaaatcaagtgtctccactgccacgggatttcagacacttttgacccttacctggacatcgccctg gatatccaggcagctcagagtgtcaAgcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat gcctatcattgcggtctttgtcttcagagggcgccagtctccaagacgttAactttacacacttttgccaaggaa cgcatacttgaaacgcagagaccatgggtggtcacacgccacaaactagccaagagtgtgcaatatgctgagagc Cctgacatgcagccatacatgtctcagcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccac gctgggtggagttgtcacgatggacAttacttctcttatgtcMagctcaagaaggccagtggtataaaatggat gatgccaaggtcactgcctgtagcatcacttctgtcctgagtcaacaggcCtatgtcctcttttacatccagaag agtgaatgggaaagacacagtgagagtgtgtcaagaggcagggaaccaagagccctcggcgctgMgacacagac Aggcgagcaacgcaaggagagctcaagagagaccacccctgcctccaggcacccgagttggacgagcgcttggtg gaaagagccactcaggaaagcacctTagaccactggagattcccccaagagcaaaacaaaacgaagcctgagttc aacgtcagaaaagtcgaaggtaccctgcctcccaacgtacttgtgattcaTcaatcgaaatacaagtgtgggatg aaaaaccatcatcctgaa Cagcaaagccccctgctaaacctctcttcgacgacccggacagatcaggagtccgtg aacactggcaccctcgcttctccaaggcracigaccagcragatccaaagggaagaacaaacacagcaagagggct ctgcttgtgtgccagtga ΚΟΟΒ8·5 deducting the peptide sequence to -74- green This paper scale applies to the Chinese National Standard (CNS) A4 specification (210 X 297 public) A7 B7 1312008 V. Invention description (73)

ΜΕΟΟείΛΌΟΟΕν ^ ΝΗΓεΚΙ / ΓεΗίΙΡΟΑΑΡΑΕΙΟΕΠ ^ ΡΕΚεΡΙ ^ ΕΑΗνϋΓΧΙΟΟΙΑΡνΑΚΟίΑΡΚΚΚΙιΡΙ ^ ΚΚΡ ΑΑνσΑσί ^ ΝΜΟΝΤΟΥΕΝΑε ^ Οαί / ΓΥΤΡΡΙΑΝΥΜΧ ^ ΚΕ ^ ΟΤαΟΙΙΡίαταΜΙΛΤΓΜΟΑΗΓηίΑΙ ^ ΚίΗνίΟΡβΟΑΙΑ ΑΟΡΉΪ ^ ΚΟΕΟΑΗΕΡΙΛΡΤνΠΑΜΚΚΑΟΧΡΟΗΚΟνΟΗΗεΚΟΤΤΙ ^ ΗΟΙΓσσσίίΚεΟΙΚίΧΗεΗΟυΟΤΡΟΡΥωίΑΙ * DXQAAQSVKQALEOLVKPEELNGENAyHCGLCLQRAPVSKTLTLHTFAKERILETQRPWWTRHKIAKSVOYAES 5 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKMDDAKVTACSITSVLSQQAYVX.FYIQK SEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDERLVERATQESTLDHWRFPQEQNKTKPEF ΝνΚΚνΕ〇ΊΧί > ΡΝνΐΛ / ΙΗ〇5ΚΥ1 «: σΜΚΝΗΗΡΕ〇〇 53ΙΧΝΙ^5ΤΤΚΤΟ<5Ε3νΝΤ〇ΤΙΑ5Ι^ΚΤΚΚ5ϊ«3ΚΝΚΗ5ΚΚΑ LLVCQ HDUB8-6 nucleoside sequence 10 ACggaagacgactcactctatttgggaggtgactggcagttcaatcactCttcaaaactcacatcttctcggcta gacgcagctttcgctgaaatccagcGgacttctctctctgaaaagtccccctctcatctgaga cccgtttcgac ctctgtgatgatctggctcccgtggcaagacagcttgctcccagggagaaGcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaagataggaaataccttctatgtgaacgtccccctgcagtgcctgacatac 15 Acactgccgctttccaactacatgctgtcccgggaggactctcaaacgtgtcatcttcacaagtgctgcatgttc tgtactatgcaagctcacatcacacGggccctctaccgccctggccatgtcatccagccctcacaggtattggct gctggcttccatagaggtgagcaggaggatgcccatgaatttctcatgttTactgtggatgccatgaasuiaggca tgccttcccgggcacaagcagccagatcatcactccaaggacaccaccctcatccaccaaatatttggagcgtat Tggagatctcaaatcaagtatctccactgccacggcatttcagacacctttgacccttacctggacatcgccctg 20 gatatccaggcagctcagagtgtcaAgcaagctttggaacagttggtgaagcccaaagaactcaatggagagaat gcctatcattgtggtctttgtotccagaaggcgcctgcctccaagacgttAactttacccacCCctgocaaggtc ctcattcttgtattgaagagattctccgatgtcacaggcaacaaacttgccaagaatgtgcaatatcctaagtgc Cgtgacatgcagccatacatgtctcagcagaacacaggacctcttgtctatgtcctctatgctgtgctggtccac gctgggtggagttgtcacaacggacAtcacttctcttatgtcaaagctcaagaaggccagtggtataaaatggat 25 gatgccgaggtcactgcctctggcatcacctctgtcctgagtcaacaggcCtatgtcctcttttac atccagaag agtgaatgggaaagacacagtgagagtgtgtcaagaggcagggaaccaagagcccttggtgctgaagacacagac Aggccagcaacgcaaggagagctcaagagagaccacccttgcctccaggtacccgagttggacgagcacttggtg gaaagagccactcaggaaagcacctTagaccactggaaattcccccaaaagcaaaacaaaacgaagcctgagttc aacgtcagaaaagttgaaggtaccctgcctcccaacgtacttgtgattcaTcaatcaaaatacaagtgtggtatg 30 aaaaaccatcatcctgaacagcaaagctccctgctaaacctctcttcgacgaaaccgacagatcaggagtccatg Aacactggcacactcgcttctctgcaagggagcaccaggagatccaaagggaataacaaacacagcaagagatct ctgcttgtgtgccagtga 35 interpretation of the polypeptide sequence

ΜΕΟΟεί ^ ΥΙΛΟΙΛ ^ ΓΝΗΓεΚΙ ^^ ΚΙιΟΑΑΡΑΕΙΟΙΙΤδΙ ^ ΕΚεΡΙ ^ εΤΗΡΟΙίαΗίΙΑΡνΑΚΟΙΑΡΚΕΚυΡΙ ^ ΗβΡ ΑΑνΟΑΟΙ ^ ΚΙΟΝΤΕΎνΐίνεΐ ^ ΟΟΙ / ΓΥΤΙ ^ βΝΥΜΙ ^ ΚΕΟεΟΤΟΗυααταΜΡΟΓΜΟΑΗΙΤΜΑ ^ ΥΚΡΟΗνίΟΡεονίΑ 40 ΑΟΡΗΙ ^ ΕΟΕΟΑΗΕΡωίΡτνΟΑΜΚΚΑί ^ ΡσΗΚΟΙΛΗΪΚΚϋΤΤΙ ^ ΙΗΟΙΡΟΑΥνίΚεςίΙΚΥωαΜίεΟΤΡϋΡΥΧ ^ ΙΑΙ * DIQAAQSVKQALEQLVKPKEl ^ ENAyHCGI ^ LQKAPASKTLTLPTSAKVLIX / VIiKRFSDVTGNKXAKNVQYPKC RDMQPYMSQQNTGPLVYVXaYAVLVHAGWSCHNGHYFSYVKA.QEGQWYKMDDAEVTASGITSVLSQQAWLFYXQK βΕ ^ ΗΗβΕβνεΐ^ΗΕΡΚΑΙ^ΑΕϋτΟΚΡΑΤΟσΕΙιΚΚϋΗΡα^νΡΕΙιηΕΗ^νΕΚΑΤΟΕεΤΙΛΗνίΚΕΡΟΚΟΝΚτΚΡΕΡ NVRKVEGTLPPNVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTKPTDQESMNTGTLAStiQGSTRRSKGNNKHSKRS 45 LLVCQ HDOB8«7 Soylinic Acid Sequence Ministry Intellectual Property Bureau Staff Consumer Cooperative Printed

Atggaggacgactcactctacttgggaggtgagtggcagttcaaccacttttcaaaactcacatcttctcggcca 50 gaCgcagcttttgctgaaatccagcGgacttctccccctgagaagtcaccactctcatctgaggcccgtgtcgac cCctgtgatgatttggctccCgtggcaagacagcttgctcccaggaagaaGcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaatatgggaaataccCgctacgagaacgcttccctgcagtgcGtgacatac Acaccgccccttgccaactacatgctgtcccgggagcactctcaaacatgtcagcgtcccaagtgctgcatgctc tgtactatgcaagctcacatcacatGggccctccacagtcctggtcatgtcatccagccctcacaggcattggct 55 gctggcttccacagaggcaagcaggaagatgcccatgaaCttctcatgtCCactgtggatgccatgaaaaaggca tgccttcccggccacaagcaggtagatcatcactctaaggacaccaccctcatccaccaaatatttggaggctgc Tggagatctcaaatcaagtgtctccactgccacgggatttcagacacttttgacccttacctggacatcgccctg gatatccaggcagctcagagtgtcaAgcaagctttggaacagttggtgaagcccgaagaactcaatggagagaat gcctatcattgcggtctttgtctccagagggcgccagcctccaagacgttAactttacacacttctgccaaggtc 60 ctcatccttgtcttgaagagattctccgatgtcacaggcaacaaacttgccaagaatgtgcaatatcctgagtgc -75- This paper scales applicable Chinese National Standard (CNS) A4 size (210 X 297 mm) A7 B7 1312008 V. Description of invention (74

CttgacatgcagccatacatgCctcagcagaacacaggacctcttgtctatgtccCctatgctgtgctggtccac gctgggtggagCtgCcacgatggacAttacttctcttatgtcaaagctcacigaaggccagtggtataaaacggat gatgccaaggccactgcctgtagcatcacttctgtcctgagtcaacaggcCtatgtcctcttttacatccagaag agtgaacgggaaagacacagtgagagcgtgtcaagaggcagggaaccaagagccctcggcgctgaagacacagac

AggcgagcaacgcaaggagagctcaagagagaccacccccgccCccaggcacccgagttggacgagcgcttggtg gaaagagccactcaggaaagcacctTagaccaccggagattcccccaagagcaaaacaaaacgaagcctgagttc aacgtcagaaaagtcgaaggtaccctgcctcccaacgtactcgtgatccaTcaaCcgaaatacaagtgtgggacg aaaaaccatcaccctgaacagcaaagctccctgctaaacctctcttcgacgacccggacagatcaggagtccgtg aacactggcaccctcgcttctctgcaagggaggaccaggagatccaaagggaagaacaaacacagcaagagggct 10 ctgcttgcgtgccagtga HDOB8.7 polypeptide sequence interpretation 15 20

AAVGAGLQNMGNTCYENASLQCIiTYTPPIaANYMljSREHSQTCQRPKCCMLC, AGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGCWRSQIKCLHCHQISDTFDPYI ΟϊΟΑΑί ^ νΚΟΑΙ ^ Εζ ^ νΚΡΕΕΙ ^ ΝΟΕΝΑΥϊΚ ^ Ι ^ Ι ^ ΚΑΡΑΒΚΤΙ / Π ^ ΗΤΞΑΚνΐ ^ ENGINEERING LVLKRFSDVT < iKLAKKfVQY * PEC LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKiiDDAKVTACSXTSVLSQQAYVLFYIQK SEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDERLVERATQESTLDHWRFPQEQNKTKPEF NVRKVEGTLPPNVLV station ΗζβΚΥΚΟΟΜΚΝΗΗΡΕζΧ ^ Ι ^ ΝΤ ^ ΤΤΚΤϋΟΕδνΝΤΟΤΙ ^ Ι ^ ΟαίΙΤΚΙ ^ ΚΟΚΝΚΗεΚΚΑ LLVCQ:? TM 〇AHITWALHSPGHVlQPSi

SQALA LDIAL 25 30 35 45 HDDBSe8 nucleotide sequence AtggaagacgactcactctatttgggaggCgactggcagttcaatcacttttcaaaactcacatcttctcggcta gatgcagcctttgctgaaatccagcGgacttctctctctgaaaagtcaccactctcatctgagacccgtttcgac ctctgcgatgatttggctcctgtggcaagacagcttgctcccagggagaaGcttcctctgagtagcaggagacct gctgcggtgggggctgggctccagaagataggaaataccttctatgtgaacgtttccctgcagtgcctgacatac Acaccgccgctttccaactacatgctgtcccgggaggactctcaaacgtgtcatcttcacaasftgctgcatgttc tgtactatgcaagctcacatcacatGggccctctaccgtcctggccatgtcatccagccctcacaggtattggct gctggcttccatagaggt ^ agcaggaggatgcccatgaatttctcatgttTactgtggatgccatgaaaaaggca tgccttcccgggcacaagcagctagatcatcactccaaggacaccaccctcatccaccaaatatttggagcgtat TggagatctcaaaCcaagtatctccactgccacggcatttcagacacctttgacccttacctggacatcgccctg gatatccaggcagctcagagtgtcaAgcaagctttggaacacrC.t9gtgaagcccaMgaactcaatggagagaat gcctatcattgtggtctttgtctccagaaggcgcctgcctccaagacgttAactttacccacttictgccaaggtc ctcaCtcttgtattgaagagattctccgatgtcacaggcaacaaacttgccaagaatgtgcaatatcctaagCgc Cgtgacatgcagccaitacatgtctcagcagaacacag gacctcttgtctatgtcctctatgctgtgctggtccac gatgccgaggtcactgcctctggcatcacctctgtcctgagtcaacaggcCtatgtcctcttttacatccagaag agtgaacgggaaagacacagtgagagtgtgtcaagaggcagggaaccaagagcccCtggtgctgaagacacagac Aggccagcaacgcaaggagagctcaagagagaccacccttgcctccaggtacccgagttggacgagcacttggtg gaaagagccactcaggaaagcacctTagaccactgoaaattcccccaaaagcaaaacaaaacgaagcctgagttc aacgtcagaaaagttgaaggtaccctgcctcccaacgtacttgCgattcaTcaatcaaaacacaagtgtggtatg aaaaaccatcatcctgaacagcaaagotccgtgctaaacctctcttcgacgaaaccgacagatcaggagtccatg aacactggcacactcgcttctctgcaagggagcaccaggagatccaaagggcLataacaaacacagcaagagatct: ctgcttgtgtgccagtga booked 50 Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed BDOBLe play whoof polypeptide sequence

MEDDSLYLGGDWQFNHPSKLTSSRLDAAFAEIQRTSLSEKSPLSSETRFDLCDDLAPVARQLAPREKLPLSSRRP AAVGAGLQKIGNTFYVNVSLQCLTYTLPLSNYMLSRBDSQTCHLHKCCMPCTMQAHITWALYRPGHVIOPSQVIA 55 agfhrgeqedaheflmftvdamkkaclpghkqldhhskdttlihqifgaywrsqikylhchgisdtfdpyldial ΟΙΟΑΑΟδνΚΟΑ ^ ΕΟίνΚΡΚΕΙΛΙΟΕΝΑΥΙΚ ^ ΙΛΙ ^ Κί ^^ ΚΤυΓυ ^ ΑΚνίίΙΙ ^ ΚΙΙΡεΒντσΝΚΙΑΚΝνΟΥΡΚΟ RDMQFYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKAQEGOWYKMDDAEVTASGITSVLSQQAYVLFYXQK SEWERHSESVSRGREPRALGAEDTDRPATQGELKRDHPCLQVPELDEHXiVERATQESTLDHWKFPQKQNKTKPEF ΝνΚΚνΕσΤΙ ^ ΡΡΝνίΛ / ΤΗζ ^ ΚΥΚαΜΚΝΗΗΡΕΟί ^ ενί ^ δδΤΚΡΤΟςΕεΜΝΤΟΤΙΑδΙ ^ ΟΟδΤΚΚεΚΟίΝΚΗΞΚΚΒ 60 LLVCQ -76- This paper scales applicable Chinese National Standard (CNS) A4 size (210 .X 297 mm) Ministry of Economic Affairs Intellectual Property Bureau Staff Consumption Cooperative Printed 1312008 V. Inventions (75) HDOB8.il nucleoside sequence 5 AtggaggacgactcactctacttgggaggtgagtgggagttcaaccacttttcaaaactcacatcttcCcggcca gatgcagcctttgctgaaatccagcGgacctcCccccctgagaagtcaca actctcaactgagacccgcgccgac ttotgcgatgatttggccgctgtggcaagacagctcgctcccagggagaaGcttcctccgagtagcaggagacct gctgcggtgggggctgggctccagaatatgggaaatacctgctacgtgaacgcttcccagcagtgtctgacatac Ataccgccccttgccaaccacatgctgtcccgggagcactctcaaacatgtcatcgtcacaagtgctgcatgccc 10 tgtaccatggaagccoacatcacaCGgcccctccacattcctggccatgtcatccagccctcacaggcaccggct: gctggctCccatagaggcaagcaggaagccgcccttgaatttctcatgttCactgtggatgccacgaaaaaggca tgccttcccgggcacaagcagacccccacccccgtatggaagagattctccgatgtcacaggcaacaaaattgcc Aagaatgtgcaatatcctgagtgccctgacatgcagccatacatgtctcagcagaacacaggacctcttgtctat gtcctctatgcCgtgctggtccacgCcgggtggagttgtcacaacggacattacttctcttatgtcaaagttcaa 15 gaaggccagCggtacaaaatggat: gat; gccgagctagagtgaatgcrgaaagAcacagtgagagtgtgt: caagaggc agggaaccaagagccctcggcgctgaagacacagacaggcgagcaacgcaaggagagctcaagagagactacccc TgcctccaggtacccgagtCggacgagcacttggtgsraaagagccactcaggaaagcaccttagaccactggaaa ttcctccaagagcaaaacaaaacgaAgcccgagttcaacgtcagaaaacttgaaggtaccctgcctcccaacgta ctcgtgattcatcaatcaaaatacaacrtgtgggatgaaaaaccatcat ccTgaacagcaaagccccctgctaaac 20 ctctcttcgacgaacccgacagatcaggagtccatgaacactggcacactcgcttctctgcaagggaggaccagg agatccaaagggaagaacaaacactgcaagagggctctgcttgtgCgccagtga HOTB8,1:L deduced polypeptide sequence 25

MEDDSLYI,GGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSQLSTETRVDFCDDLAAVARQLAPREKLPLSSRRP aavgaglqnmgntcyvnasqqcltyipplanymlsrehsqtchrhkccmlctmeahitwplhipghviqpsqala

AGFHRGKQEAALEFLMFTVDAMKKACLPGHKQILILVWKRFSDVTGNKIAKNVQYPECIiDMQPYMSQQNTGPLVY VLYAVLVHAGWSCHNGHYFSYVKVQEGQWYKMDDAEKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDYP 30 CLQVPELDEHLVEHATQESTLDHWKFLQEQNKTKPEFNVRKLEGTLPPNVLVIHQSKYKCOiKNHHPEQQSSLLN LSSTNPTDQESMNTGTLASLQGRTKRSKGXNKHCKRALLVCQ HD0B4 * 4 nucleotide sequence 35 atggaggagg actcactcta cttgggtggt gagtggcagt tcaaccactt ttcaaaactc acatcttctc ggctcgatgc agcttttgct gaaatccagc ggacttctct ccctgagaag tcaccactct catgt ^ agac ccgtgtcgac ctctgtgatg atttggttcc rgaggcaaga cagcttgccc ccagggagaa gcttcctctg agtagcagga gacctgctgc ggtgggggct 40 gggctccaga atatgggaaa tacctgctac gtgaacgctt ccttgca9tg cctgacatac acaccgcccc ttgccaacta catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac aagggctgca Cgctctgtctc tatgcaagct cacatcacac gggccctcca caatcctggc cacgtcatcc agccctcaca ggcattggct gctggcttcc atagaggcaa gcaggaagat gcccatgaat ttctcatgtt cactgtggat gccatgaaaa aggcatgcct tcccgggcac 45 aagcaggtag atcatccctc taaggacacc accctcatcc accaaatatt tggaggctac tggagatctc aaatcaagtg tctccactgc cacggcattt cagacacttt tgacccttac ctggacatcg ccctggatat ccaggcagct cagagtgtcc agcaagcttt ggaacagttg gtgaagcccg aagaactcaa tggagagaat gcctatcatt gtggtgtttg tctccagagg gcgccggcct ccaagacgtt aactttacac acttctgcca aggtcctcat ccttgtattg 50 aagagattct ccgatgtcac aggcaacaag attgccaaga atgtgcaaCa tcctgagtac cttgacatgc agccatacat gtctcagcag aacacaggac ctcttgtcta tgtcctctat gctgtgctgg tccatgctgg gtggagctgt cacaacggac attacttctc ttatgCcaaa gcCcaagaag gccagtggta taaaatggat gatgccgagg tcaccgcctc tta ^ catcac atctgtcctg agtcaacagg cctacgtcct cttCtacatc cagaagagtg aatgggaaag 55 acacagtgag agtgtgtcaa gaggcaggga accaagagcc cttggcgcag aagacacaga caggcgagta acgcaaggag agctcaagag agaccacccc tgactccagg cccccgagtt ggacgagcac ttggcggaaa gagccactca ggaaa9〇acc ttagaccact ggaaattcct ccaagagcaa aacaaaacga agcctgagtt caacgtcaga aaagtcgaag gtaccctgcc tcccgacgta ctCgtgattc atcaatcaaa atacaagtgt gggatgaaga accatcatcc 60 tgaacagcaa agctccctgc taaacctctc ttcgacgacc ccgacacatc agcagtccat -77- scale sheet of the present Applicable National Standards (CNS) A4 size (210 X 297 mm)

1312008 A7 B7 V. Inventions (76) gaacaacggc acactcgctt ccctgcgagg gagggccagg agatccaaag ggaagaacaa acacagcaag agggctctgc ttgtgtgcca gtga

MEEDSLYLGG QLAPREKLPL KGCMLCTMQA KQVDHPSKDT VKPEELNGEN LDMQPYMSQQ

EWQFNHFSKL TSSRLDAAFA EIQRTSLPEK SPLSCETRVD LCDDLVPEAR SSRRPAAVGA GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH HITRALHNPG HVIQPSQMA AGFHRGKQED AHEFLMFTVD AMKKACLPGH TLIHQXFGGY WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL AYHCGVCLQR APASKTLTLH TSAKVLILVI * KRFSDVTGNK IAKNVQYPEC NTGPLVYVLV AVLVHAGWSC HNGHYFSYVK AQEGQWYKMD DAEVTAS 15 hDUBta Yi nucleotide sequence 20 25 30 35 atggaggacg actcactcta cttgggaggt gagtggcagt tcaaccactt ttcaaaactc acatctcctc ggcccgatgc agcttttgct gaaatccagc ggacttctct ccctgagaag tcaccactct catgtgagac ccgtgtcgac ctctgtgatt atttggctcc cgtggcaaga cagcttgctc ccagggagaa gcttcctctg agtagcagga gacctgctgc ggtgggggct gggctccaga atatgggaaa tacctgctac gtgaacgctt ccttgcagtg cctgacatac acaccgcccc ttgccaacta catgctgtcc cgggagcact ctcaaacgtg tcatcgtcac aagggctgca tgctctgtac tatgcaagct cacatcacac d gccctcca caatcctggc cacgtcat.cc agccctcaca ggcattggct gctggcttcc atagaygcaa gcaggaagat gcccatgaat ttctcatgtt cactgtggat gccatgaaaa aggcatgcct tcccgggcac aagcaggtgg atcatcactc Taaggacacc accctca tcc accaaacatt tggaggctac tggagatctc aaatcaagtg tctccactigc cacggcattt cagacacttt tgacccttac ctggacatcg ccctggatat ccaggcagct cagagtgtcc agcaagcttt ggaacagttg gtgaagcccg aagaactcaa tggagagaat gcctatcatt gtggtgtttg tctccagagg gcgccggcct ccaagacgtt aactttacac acctctgcca aggtcctcat ccttgtattg aagagattct ccgatgtcac aggcaacaag attgccaaga atgtgcaata tcctgagtgc cttgacatgc agccatacat gtctcagcag aacacaggac cccttgtcta tgtcctctat gctgtgctgg tccacgctgg gtggagttgt cacaacggac attacttctc ttatgtcaaa gctcaagaag gccagtggta taaaattgat gatgccgagg tcaccgcctc tagcatcact tctgtcctg this ctcaacaggc ctacgtcctc ttttacatcc agaagagtga atgggaa ancient ga cacagtgaga gtgtgtcaag aggcagggaa ccaagagccc ttggctctga agactaa booked 45 play whoof polypeptide sequence MEDDSLYLGG EWQFNHFSKL TSPRPDAAFA EIQRTSLPEK SPLSCETRVD LCDYLAPVAR QLAPREKLPL SSRRPAAVGA GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH KGCMLCTMQA HITRALHNPG HVIQPSQALA AGFHRGKQED AHEFLMFTVD AMKKACLPGH KQVDHHSKDT TLIHQIFGGY WRSQIKCLHC HGISDTFDPY LDIALDIQAA QSVQQALEQL VKPEELNGEN AYHCGVCIiQR ΑΡΑεΚΤΙιΤΙ^Η TSAKVLILVL KRFSDVTGNK IAKNVQYPEC LDMQPYMSQQ NTGPLVYVLY AVLVHAGWSC HNGHYFSYVK AQEGQWYKID DAEVTASSIT SVLTQQAYVL FYIQKSEWER HSESVSRGRE PRALGSED hDOB8.2 Nucleotide sequence

I Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 50 atgcggccag agagcccgtc atttgaagac ccggaagaga tagcgtcttt ctgcaacctg cggtcccagc cgaaaaacct tgtgatcctt gttccgggcg acatggagga cgactcactc tacttgggag gtgagtggca gttcaaccac ttttcaaaac tcacatcttc tcggccagat gcagcttttg ctgaaatcca gcggacttct ctctctgaga agtcatcact ctcatctgag acccgcgtcg acctctgtga tgatttggct cctgtggcaa gacagctcgc tcccagggag 55 aagcttcctc tgagtagcag gagacctgct gcggtggggg ctgggctcca gaatatggga aatacctgct acgtgaacgc ttccctgcag tgcctgacat acacaccgcc ccttgccaac tacatgctgt cccgggagca ctctcaaacg tgtcatcgtc acaagtgctg catgctctgt actatgcaag ctcacatcac atggcccctc cacagtcctg gccatgtcat ccagccctca caggtgttgg ctgctggctt ccatagaggc gagcaggaag atgcccatga atttctcatg -78- this paper scales applicable Chinese national standard (CNS) A4 size (210 X 297 mm) 1312008 economic Affairs intellectual property Bureau employee consumption cooperative printing 5, invention description (77) tccactgtgg atgccatgaa aaaggcattc cttcccgggc acaagcattt agataatcac cctaaggaca ccaccctcac ccaccaaata cttggagggt actgg agatc tcacaCcaac tgtttccacc gccacgggat ttcagacacc tctgaccctt acctggacat cgccctggat atccaggcag ctcagagtgt caagcaagct ttgtaacagc tggcgaagcc cgaagaactc 5 aacggataaa aCgcctatca Ctgtggtctt tgtctccaga aggcgcctgc ctccaggacg Ctaactttac acacttcCgc caaggtcctc atccttgtaC tgaagagatt ctctgaggtc acaggcaaca aacttgccaa gaatgcgcaa tatcctgagt gccctgacat gcagccatac atgtctcagc agaacacagg acctcctgtc tatgtcctct atgccgtgct ggtccacgct gggcggagtt gtcacaacgg acattactta tcttatgtca aactcaagaa ggccattggt 10 ataaaatgga tgatgccgag gtcactgcct ccggtatcac tCctgtcctg agtcaacagg cctatgtcct ctCttacatc cagaagaatg aattcggaag acccagttac agtgtgtcca taggcaggga accaagagct ctctgcgtga aggcaagtga attgtgtgtg aaataaaatg tcatgaaCaa atcttgcagt ggagtattta tttgtctcac tttgtaatca gtgaatgagc tttaaccaat atcaatgcct agtgcctacc ccccagagat aagaacttcc actctcttat 15 gtgtaaccat ggcctcCgga ttgcttatga ctctgaagat aattctcctt tcccccaacg ttccagaatc acttcaggtg gtggtaacag acaacacatc agtccctttc tctctctttt ctcttcactc aggaaaactc tcactgagac aaaggaaaat cctatgg ttt actggggagg aagaattccc tcaggagtga aattggtggc tccttcctcc ctgtcaagtc tcttcctcag gattgcccct ttgtctcttc aggact 20 * 2 Chán hD〇B8 Yi polypeptide sequence MRPESPSFED SEEXASFCNL RSQPKNLVIL VPGDMEDDSL YLGGEWQFNH FSKLTSSRPD 25 AAFAEIQRTS LSEKSSLSSE TRVDLCDDLA PVARQLAPRE KLPLSSRRPA AVGAGLQNMG NTCYVNASLQ CLTYTPPLAN YMLSREHSQT CHRHKCCMLC TMQAHITWPL HSPGHVIQPS QVLAAGFHRG EQEDAHEFLM FTVDAMKKAF LPGHKHLDNH SKDTTLIHQI FGGYWRSHIN CFHCHGISOT FDPYLDIALD IQAAQSVKQA L 30 hDQB 8 · 9 remove glycoside blind sequence atggaggaag actcactcta cttgggaggt gagtggcagt tcaaccactt ttcaaaactc 35 acatcttctc agccagatgc agcttttcct gaaatccagc ggacttctct ccctgagaag tcaccactct catcggagac ccgtgtcg-ac ctctgtgacg atttggctcc tgtgacaaga cagcttgctc ccagggagaa gcttcctccg agtagcagga gacctgctgc ggtgggagct ggtctccaga atatgggaaa tacctgccac ttgaacgctt ccctgcagtg cctgacatac acaccgcccc ttgccaacta catgctgtcc tgggagctct ctcaaatgtg tcatcgtccc 40 aagtgctgca Tgctctgtat tatggaagct cacagcacac gggcacctcc accgtcctgg ccatgtcacc cagccctcac aggcattggc tgctgacttc catagagaca agcag gaaga tgcccatgaa tttctcatat tcactgtgga tgccattaga aaggcatgcc ttcccgggca caagcagcta gatcatcact gcaaggacac catcctcatc caccaaatat ttggagggta ctagagatct caaatcaagt gtctctactt ccacggcatt tcagacacct tcgaccctta 45 cctggatatc gccctggata tccaggcagc tcagagtgtc aagcaagctt tggaacagtt ggtgaagccc gaagaactca atggagagaa tgcctatcat tgtggtcttt gtctccagaa ggcgcctgcc gccaagacgt taactttacc cacttctgcc aaggtcctca tccttgtctt gaagagattc tccgatgtca caggcaacaa acttgccaag aatctgcaat atcctgagtg cgttgacatg cagccataca tgcctcagca gaacacagga cctcttttct atgtcctcta 50 tgctgttctc gtcatcaccg ggtggagttg tcacaacgga cattacttct cttgtgtcaa actcaagaag gccagtggta taaaatggat gatgccgagg tcactgcctc tggtatcact gtcaacaggc ctatgtcctc ttttacatcc agaagaatga atttggaaga cccagttaca gggtgtccgc aggcagagaa ccaagagctc tttgtgctga agacaattga attgtggtga aataatatgt catgaataaa tcttgcagca gatttatttg tctcactttg 55 taatcagtga atgagcttta acgaatatca atgcctagtg cctacccccc agagataaga acttccagtt tctcatgtgt aatcatggca tctggattgc tcatgattct gaagat tctccttaga aaCt ctcctgtccc ccaaagtttc agaatcactt caggtggtag aaacagataa cacatcagtc cctttctctc tcttttctct tea 60 绎 polypeptide sequence -79-

This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention description (78)

MEDDSLYLGG EWQFNHFSKL TSSRPDAAFA EIQRTSLSEK SSLSSETRVD LCDDLAPVAR QLAPREKLPL SSRRPAAVGA GLQNMGNTCY VNASLQCLTY TPPLANYMLS REHSQTCHRH KCCMLCTMQA HITWPLHSPG HVIQPSQVLA AGFHRGEQED AHEFLMFTVD AMKKAFLPGH 5 KHLDNHSKDT TLIHQIFGGY WRSHINCFHC HGISDTFDPY LDIALDIQAA QSVKQALEQL VKPEELNG hDUB 8,10 nucleotide sequence atggaggacg actcactcta cttgggaggt gagtggcagt tcaaccactt ttcaaaactc 10 acatcttctc ggccagatgc agcttttgct gaaatccagc ggacttctct ctctgagaag ccatcactct catctgagac ccgcgtcgac ctctgtgatg atttggctcc tgtggcaaga cagctcgctc ccagggagaa gcttcctctg agtagcagga gacctgctgc ggtgggggct gggctccaga atatgggaaa cacctgctac gtgaacgctt ccctgcagtg cctgacatac acaccgcccc ttgccaacta catgctgtcc cgggagcact cccaaacgtg tcatcgtcac 15 aagtgctgca tgctctgtac catgcaagct cacatcacat ggcccctcca cagtcctggc catgtcatcc agccCtcaca ggtgttggct gctggcttcc atagaggcga gcaggaagat gcccatgaat ttctcatgtt cactgtggat gccatgaaaa aagcattcct tcccgggcac aagcatttag ataatcactc taaggacacc accctcatcc accaaatatt tggagggtac tggagatctc acatcaactg tttccactgc c atgggattt cagacacctt tgacccttac 20 ctggacatcg ccctggatac ccaggcagct cagagtgtca agcaagcttt ggaacagttg gcgaagcccg aagaactcaa tggataaaat gcctatcatt gtggtctttg cctccagaag gcgcctacct ccaggacgtt aactttacac acttctgcca aggtcctcat ccttgtattg aagagattct ctgatgtcac aggcaacaaa cttgccaaga atgtgcaata tcctgagtgc cttgacatgc agccatacat gtctcagcag aacacaggac ctcttgtcta tgtcctctat 25 gctgtgctgg tccacgctgg gtggagttgt cacaacggac attacttatc ttatgtcaaa ctcaagaagg ccattggtat aaaatggatg atgccgaggc cactgcctcc ggtatcactt ctgtcctgag tcaacaggcc tatgtcctct tttacatcca gaagaatgaa tttggaagac ccagttacag cgtgtccata ggcagggaac cgagagctcc ttgcgtgaag gcaagtgaat tccctttctc tctcttttct cttcactcag gaaaactctc actgagacaa aggaaaatcc tatggtctac tggggaggaa gaattccctc ag tgtgtgtgaa ataaaatgtc atgaataaAt cttgcagtgg agtatttatt tgtctcactt 30 tgtaatcagt gaatgagctc taaccaatat caatgcctag tgcctacccc ccagagacaa gaacttccac tctcttatgt gtaaccatgg cctctggatt gcttatgact ctgaagataa tcctcctttc ccccaacgtt tcagaatcac tccaggtggt ggtaacagat aacacaccag Gagtgaaa ttggtggctc cttcctccct 35 gtcaagtctc ttcctcagga ttgccccttt gtctcttcag gactctgctc atcaggcccg agatgccccc tggtcgtgca tacctggcct gt.gaagaaat a hD0B8 · 1G deduced polypeptide sequence 40

DSLYLGG PREKLPP

EWQFNHFSKL TSSQPDAAFP EIQRTSLPEK QLAPREKLPP SSRRPAAVGA GLQNMGNTCH LNASLQCLTy KCCMLCIMEA HSTRAPPPSW PCHPALTGXG C SPLSSETRV] TPPLANYML;

LCDDLAPVTR S WELSQMCHRP 45 Table 24. hDUB4.10舆hDUB4.11 漭绥Amino Acid Sorting by the Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 50 55 60 HDUB4 · 10 MCIRTGSPCDVCENYSVMSMTGRQLIDWAPUCIGYEHSSTPMPREHVHFRQHYNFGTKCA 60 hDUB4.11 MCXRTGSPCDTCENYSVMSOTGRQLIDWAPLKIGYEHSSTPMPRT-LYIRHRK----- --52 俞会*食***#食鸟喻贪"Fei Huo threatening threat ★Food Yu ★★食贪心鸟★贪青食食食 w#食喻·.·*·» hDUB4.10 NCNSTIQCVTGNGGWIADPERSMRESRXCTAYFGLFPLKQGPVLKMVISLGQRINRLNVE 120 hDUB4.ll---------PSDGAHLAHEK------------------------------------ -TRE 66 hDUB4.10 RLSLEGKKIRCAKYYTSLTILRSESALSTSCPSVAERMMAAAKRIASFCNLRSQQKNLVI 180 hDUB4.11 RNGAKGKKIRCAKYYTSLTXLRSESALSSSCPSVAERMMAAAK-----------------109 * ♦*#«* 会#♦«俞*«♦*««胁*« * Threat* Greedy • Will **** «♦ ** # 会♦♦食 hDUB4.10 LVPVDMEDDSLYI-GGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDL 240. This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7B7 V , invention description (79) hDUB4.11 - IDMEDDSLYLGGEWQFNHFSK LTSSRPDAAFAEIQRTSLPEKSPT.S-, the club will meet "*★*♦★*会'★♦«****★♦♦♦♦*#**** 喻★★会* * -YDL 158 hDUB4 · 10 ΑΡνΑΚΟΙΑΡΚΕΚΙ ^Ι^ΚΚΡΑΑνσΜΙ^ΝΜΟΝΤΟΥνΝΑΒΙ^ΟΟΙ/ΓΥΤΤΡΙΑΝΥΜΙ^ΕΗες} 300 hDUB4.11 APVARQLAPREKLPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYTPPLANYMLSREHSQ 218 ★ will greedy window * 黉 ★ ★ food ★ 亩 ★ 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 卖 ★ ★ ★ ★ ★胁 贪 胄 ★ # # # # # # ★ ★ ★ ★ ★ ★ 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 ★***会«*«★★★★★★★★★★*★★★★★★★** #**★★#*★ 15 hDUB4·ιο clpghkqvdhhskdttliHqifggywrsqikclhchgisdtfdpyldialdiqaaqsvqq 420 hDUB4 . η (ΓΙ^ΚΗΚανΌΗΗΏΚΟΤΤΙ/ ΙΗΟΙΡασΥίίΗβΟΙΚα^ΗΟΗσίβΒΤΡϋΡΥΙ^ΙΑίιΟΙΟΑΑΟδνςΚ) 333 hDUB4 · 10 ΑΙ^Ε<^ν ΚΡΕΕΙ ^ 3ΕΝΑΥΗ5σν (Χ〇ΗΑΡΑ3ΚΤΙΛ'Ι1ΗΤ5ΑΚνΐιΙΙΛ / ΙιΚΪ ^ 5Ι > νΤ < »ίΚΙΑΚΝν 480 ωϋΒ4 · 11 ALEQLVKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVL work LVLKRFSDVTGNKIAKNV 3 $ 8 20 hDUB4.10 ΟΥΡΕΟ ^ ΜΟΡΥΜεΟΟΝΤαΡΙΛ / Υνί ^ Ανί / νΗΑΟν ^ ΗΝσΗΪΤεΥνΚΑΟΕΟΟΜΥΚΜΟΟΑΕντ 540 hDUB4.11 QYPECLDMQPYMSQQNTGPLVYVLYAVLVHAEWSCHNGKYFSYVKAQEGQWYKMDDAEVT 458 ★ food The threat of threats *** 食 喻 喻 贪 * ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ 胁 胁 胁 胁 胁 胁 胁 胁 胁 胁 胁 胁 胁 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金 金AASITSVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCL· 518 ** Contribute to Greed #* threaten ★*★«**#*flank ★Dance*« Food ** gluttony ★★*★★★★***♦**★★★★*★** * Food*★★★★★★ 30 hDUB4 · 10 QAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGM 66. hDUB4.11 QAPELDEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVKGTLPPDVLVIHQSKyKCGM 578 Food for sale, food, food, responsibility, greed, ★ threat #★★««★★★★★★★*** 食喻禽«#♦**★«♦ hDUB4.10 ΚΝΗΗΡΕζΚ^Χ/^ ΝΧ^εΤΡΤΗΟΕεΜΝΤΟΤΧΜΙ^ΗΑΙίΙ^ΚσΚΝΚΜΚΗΑΙΛνΟΟ 715 hDUB4.11 KNHHPEQQSSLLNLSSSTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALL1 KNHHPEQQSSLLNLSSSTPTHQESMNTGTLASLRGRARR; SKGKNKHSKRALLVC- 632 Gold '★★★*«**★食言实霣贪35 Table 25. hDUB4.5, hDUB4.8舆hDUB8.2 nucleotides Sequence alignment (the in-frame stop codon in hDUB8.2 at the bottom line) hDUB4.5 hDOB4.S hDUB8.2 ATTTGAAGAC ATGCG-CCAGAGAGCTCGTCATTTGAAGACTCTCTCGGAAGGGATAGCGTCTTGCTGCAA 5 9 ATGCGGCCAGAGAGCCCGTCATTTGAAGA----CTCGGAAGAGATAGCGTCTTTCTGCAA 56 ** 贪曹·会**« r**«食* *★«胁* * * * #费# * 夤**»* 贪** #♦*«#»♦♦#*«会45 hDt7B4.5 hDUB4.8 hDUB8.2 CCTGCGGTCCCAGCAGAAAAACCTTGTGATCCTTGTTCCAGTCGACATGGAGGAAGACTC ACTGCGGTCCCAGCAGAAAAACCTTGTGATCCTTGTTCCAGTCGACATGGAGGACGACTC CCTGCGGTCCCAGCCGAAAAACCTTGT GATCCTTGTTCCGGGCGACATGGAGGACGACTC ★ extravagant ★ « ★ ♦ * « ★ «食* threat ♦«#*·******♦*»*★♦*#«★«★★'* -* ******** ***** * * * * * 1 1 1 11 1 50 Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 55 60 hDUB4.5 hDUB4.8 hDUBd.2 hDUB4.5 hDUB4.8 hDU68.2 hOUB4.5 hDUB4 .8 hDUB8.2

ACTCTACTTGGGAGGTGAGTGGCAGTTCAACCACTTTTCAAAACTCACATCTTCTCGGCC 17 9 ACTCTACTTGCSGAGGTGAGTGGCAGTTCAACCACTrTTCAAAACTCACATCTTCTCCSGCC 179 ACTCTACTTGGGAGGTGAGTGGCAGTTCAACCACTTTTCAAAACTCACATCTTCTCGGCC 17 S CGATGCAGCTTTTGCTGAAATCCAGCGGACTTCTCTCCCTGAOAAGTCACCACTCTCATG 239 CGATGCAGCTTTTGCTGAAATCCAGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATG 239 A < ^ T «CAGCTTTTGCT < 3AAATCCAGCCK5ACTTCTCTCTCTGAGAAGTCATCACTCTCATC 236 TGAGACCCGTGTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG 299 TCSAGACCCGTGTCGACCTCTGTGATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAG 299 SACCCGCGTCGACCTCTGTGATGATTTOGCTCCTGTGCSCAAeACAGCTCGCTCCCAG 296 TGAGACCCGCGTCGACC * ♦ ★ ♦« ♦ # ** ****** 4 # ***** ***«**«*«***»· * -81.

This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 public Chu) 1312008; V. Invention description (80) 10 15 20 25 30 35 45 50 55 HDUB4.5 hDUB4, 8 hDUB8.2 hDUB4.5 hDUB4 .8 hDUB8.2 hD(JB4.5 hDUB4.8 hDUB8.2 hDUB4.5 hDUB4.a hDU88.2 hDUB4.5 hDUB4.8 hDUBB.2 GGAGAAGCTTCCTCTGAGTAACAGGAGACCTGCTGCGGTGGGGGCTGGGCTCCAGAATAT 359 GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGTGGGGGCTGGGCTCCAGAATAT 359 3CTCCAGAATAT 356 k«·**·**** * GGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGCTGCGGT * food "<< ##-threat ***** threat: TGGGC ****** GGGAAATACCTGCTACGTGAACGCTTCCTTGCAGTGCCTGACATACACACCGCCCCTTGC 419 GGGAAATACCTGCTACGTGAACGCTTCCTTGCAGTGCCTGACATACACACCGCCCCTTGC 419 GGGAAATACCTGCTACGTGAACGCTTCCCTGCAGTGCCTGACATACACACCGCCCCTTGC 416 CAACTACATGCTGTCCCGGGAGCACTCTCAAACGTGTCATCGTCACAAGCGCTGCATGCT 479 CAACTACATGCTGT.CCCGGGAGCACTCTCAAACGTGTCATCGTCACAAGGGCTGCATGCT 479 CAACTACATGCTGTCCCGGGAGCACTCTCAAACGTGTCATCGTCACAAGTGCTGCATGCT 476 CTGTACGATGCAAGCTCACATCACACGGGCCCTCCACAATCCTGGCCACGTCATCCAGCC 53 9 CTGTACGATGCAAGCTCACATCACACGGGCCCTCCACAA TCCTGGCCACeTCATCCAGCC 539 CTGTACTATGCAAGCTCJ «^ TCACATCGCCCCTCCACAGTCCTCGCCATGTCATCCAGCC 536 CTCACAGGCATTGGCTGCTGGCTTCCATAaAGGCAAGCAGGAAGATGCCCATGAATTTCT 599 CTCACAGGCATTGGCTGCTGGCTTCCATAGAGGCAAGCAGGAAGATGCCCATGAATTTCT 599 CTCACAGGTGTTGGCTGCTGGCTTCCATAGAGGCGAGCAGGAAGATGCCCATGAATTTCT 596 hDUB4.5 CATGTTC3 ^ CTGTGGATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAGCAGGTGGA, hDUB4.8 CATGTT ^ CTGTGGATGCCATGAAAAAiGGCATGCCrrCCCGGGCACAAGCAGGTAGA, hIXJB8.2 CATGTTCACTGTGGATGCCATGAAAAAGGCATTCCTTCCCGGGCACAAGCATTTAGA ^ TCA 659 659 656 hDUB4.5 hDUB4.8 hDUB8.2 hDUB4.5 hDUB4. 8 hDUB8.2 hDUB4.5 hDUB4.8 hDOB8.2 hOUB4.S h0UB4.8 hDUB8.2 TCACTCTAAGGACACCACCCTC ^ TCCACCAAATATTTGGAGGCTACTGGAGATCTCAAAT 719 TCACTCTAAGGACACCACCCTCATCCACCAAATATTTGGAGGCTACTGGAGATCTCAAAT 719 TCACTCTAAGGACACCACCCTCATCCACCAAATATTTGCSAGGGTACTGGAGATCTCACAT 716 CAAGTGTCTCCACTGCCACGGCATTTX ^ ACACTTTTGACCCTTACCTGGACATCGCCCT 779 CAAGTGTCrCCACTGCCACGGCATTTCAGACACTTTTGACCCTTACCTGGACATCGCCCT 779 CAACTGTTTCCACTCCC ^ CGGGATTTCAGACACCTTTGACCCTTACCTGGACATCGCCCT 776 * * * *食食甫*曹贪***The meeting will be held* will be window Cao will • food * «会会#«**«****食**★*俞*喻》#俞喻会食食»★ GGATATCCAGGCi ^TCAGAGTGTCX:AGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGA 839 GGATATCCAGGCAGCrCAGAGTGTCCAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGA 839 GGATATCCAGGCAtXrrCAGAGTGTCAAGCAAQCTTTGTAACAGTTQGTQAAGCCCGAAGA 836 *»*♦«# mu "♦«**♦··会**«*#«♦«# 俞会会食卖* 食胁**舍**« **«« **# * * * ******** ACTCAATCX^GAGAATGCCTATCATTGTOGTGTTTOTCTCCASAGGGCGCCGGCCTCCAA 899 ACTCAATGGAGAGAATXXTCTATCATTGTGGTGTTTGTCTCCAGAGGGCGCCCSGCCTCCAA 899 ACTCAATGGATAAAATGCCTATC^TTGTGGTCTTTGTCTCCAGAAGGCGCCTGCCTCCftG 896 ★ ♦ Avian # #* o *会«窗#«会«会*#« *♦俞************ ******* HDUB4.5 gacgttaactttacacacctctgccaaogtcctcatccttgtattgaagaoattctccga 959 WDUB4 8 GACGTTAACTTTACACACCTCTGCCAAGGTCCTCATCCTTGTATTGAAGAGATTCTCCGA 959 KDUB8.2 GACGTTAACTTTACACACTTCTC^CAAGGTCCPCATCCTTiyPATTGAAGAGATTCTCTGA 956 Threat*» Window*★***★**# Yu Yu "*★ threat "***«»食♦** will be greedy* * Ministry of Economic Affairs Hui Property Office Staff Consumer Cooperative Printed 60 65 hDUB4.S hDUB4.8 hDUB8.2 TGTCACAGGCAACAAGATTGACAAGAATGTOCAATATCCTGAGTGCCTTGACATGAAGCT 1019 TGTGACAGGCAACAAGATTGCCAAGAATCSTGCAATATCCTGAGTaCCTTGACATGCAGCC 1019 GGTCACAGGCAACAAACTTGCCAAGAATGTGCAATATCCTGAGTGCCTTGACATGCAGCC 1016 #* * Food Threat* Fee*♦ Avian* * * ★*★★******#★ «*★«*«★★*★★«***«**♦★«* *** hDUB4.S hDUB4.8 hDUB8.2 hDUB4.5 ATACATGTCTCAGACQAACTCAGGACCTCTCGTCTATGTCCTCTATGCTGTGCTGGTCCA 1079 ATACATGTCTCAGCAGAACACAGGACCTCTTGTCTATGTCCTCTATCCTGTGCTGGTCCA 1079 ATACATGTCTCAGCAGAACACAGGACCTCTTQTCTATGTCCTCTATGCTGTGCTGGTCCA 1076 ******* ****** * * * * * 食**★' Yu "*«* 啬##*喻胁♦会#*#会"1«会♦会♦♦♦ CGCTGGGTGOAGTTGTCACAACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCA 1139 -82- The paper scale Applicable to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 kl B7 V. Invention description (81) hDUB4.8 hDUB8.2 CGCTGGGTGGAGTTGTCACAACGGACATTACTTCTCTTATGTCAAAGCTCAAGAAGGCCA 1139 CGCTGGGTGGAGTTGTCACAACGGACATTACTTATCTTATG TCAAA-CTCAAGAAGGCCA 1135 hDUB4.5 hDUB4.8 HDUB8.2 GTGGTATAAAATGGATGATGCCGAGGTCACCGCCTCTAGCATCACTTCTGTCCTGAGTCA 1199 ATGGTATAAAArTGGATGATGCCGAGGTCACCGCCGCTAGCATCACTTCTGTCCTGAGTCA 1199 TTGGTATAAAATGGATGATGCCGAGGTCACTGCCTCCGGTATCACTTCTGTCCTGAGTCA 1195 Yu Yu by '| fresh food «I eat ** ★ ** *** bulimia will eat # * #« * »« # ψ ** * ♦ «舍"«♦«#♦« ♦***♦♦«*«#« hDUB4.5 hDUB4.8 hDUB8- 2 ACAGGCCTACGTCCTCTTTTACATCCAGAAGAOTGAATGGGAAAGACACAGTGAGAGTGT 1259 ACAGGCCTACGTCCTCTTTTACATCCAGAAGAGTC3AATGGGAAAGACACAGTGAGAGTGT 12 S 9 ACAGGCCTATGTCCTCTTTTACATCCAGAAGAATGAATTTGGAAGACCCAGTTACAGTGT 1255 * 胄«*,#**# * greedy ♦ mu "***** « « greedy * * * * * * * ** ** ♦ ♦ ♦ ♦ ♦ ♦ 15 h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h h * ♦ * 噙b***«***·· «身会«#胁* * * ** 20 h0UB4.5 hDUB4.8 hDUB8.2 25 h 0UB4.5 hDl7B4.8 hDUB8.2 AGGAGAGCTCAAGAGAGACCACCCCTGCCTCCAGGCCCCCGAGTTGGACGAGCACTTQGT 1179 AGGAGAGCTCAAGAGAGACC ACCCCTGCCTCCAGGCCCCCGAGrTGGACGAGCACTTGGT 1379 AAATG---TCATGA——ATAAATCTTGCAGTGGAGTATTT-ATTTGTCTCTTTTTATAAT 13ί8 * ♦ * * * * * * * * * «&gt;* * * ♦

CTCAGGAAAG 2TCAGGAAAG fcGCACCTTAGACCACTGGAAATTCCTTCAAGAGCAAAACAA 1439 CAGTGAATGAGCTTTAACCAATATCAATGCCTAGTGCCTACCCCCCAGAGATAAGAACTT 142 8 Fresh &lt; r * A * * * 4 * * * * ★★ i, 30 hDUB4.5 hDUB4.8 hDUB8.2 AACGAAGCCTGAGTTCAACGTCAGAAAAGTCOAAGGTACCCTGCCTCCCGACGTACTTGT 1499 AACGAAGCCTGAGTTCAACGTCAGAAAAGTCQAAGGTACCCTGCCTCCCGACGTACTTGT 1499 CCACTCTCTTATGTGTAAC - -CATGGCCTC * * * * STCTOGATTGCTTATGACTCTGAAGATAATTCT 14B6 YU ♦ ★* * * * * # # #* ♦ 35 hDUB4.5 hPUB4.8 hDUB8.2 GATTCATCAATCAAAATACAAGTGTGGGATGAAGAACCATCXTCCTG-AACAGCAAAGCT 1558 GATTCATCAATCAAAATACAAGTGTGGGATGAAQAACCATCATCCTG-AACAGCAAAGCT 1558 CCTT--TCCCCCAACGTTTCAGAATCACTTCAGGTGGTa3TAACAGATAACACATCAGTC 1544 • * * * * &lt;r * * **** ★ By hJ3UB4.5 hDUB4.8 hDUB8.2 «Food and poultry* greedy 45 h0CB4.5 hDUB4.8 hDUB8.2 CCCTGCTAAACCTCTCTTCGACGACCCCGACACATCAGGAGTCCATGAACACTGGCACAC 16X8 CCCTGCTAAACCTCTCTTCGTCGACCCCGACACATCAGGAGTCCATGAACACTQQCACAC 1618 CCTTTCTCrCTCTTTTCrCTTCACTCAGGAAAACTCK^CTGAGACAAAGGWUUVTCCTA 1604 will * will ♦ threatened * * * << << will TCGCTTCCCTGCGAGGGAGGGC ----- CAGGAG ^ TCCAAAGGGAAGAACAAACACAGCAA 1673 TCGCTTCCCTGCGAGGGAGGGC ----- CAGGAGATCCAAAGGGAAGAACAAACACAGCAA 1673 TACTGGGGAGGAAGAAirCCCCTCiMSGAGTGAAATTGGTGGCTCCTTCCTCCCTGT 1664 TGGTTTA * ★ # will be 50 hDUB4.5 hDUB4.8 hDUB8.2 GAGGGCTCTGCTTGTGTG ---- CCAGTGGTCTCAGTGGAAGTACCGACCCACA 1722 GAGGGCTCTGCTTGTGTG ---- CCAGTGA------------------------1698 CAAGTCTCTTCCTCAGGATTGCCCCTTTGTCTCTTCAGGACT-----------1706 * * ##*« «会《 * * ♦ I # Ministry of Economic Affairs Intellectual Property Bureau Employees Consumption Cooperatives Printed 55 60 65 Table 26. hDUB4_5, hDUB4.8 and KDUB8.2 derivatization amino acid ratio (N-terminal potential grain line «guide sequence) at the bottom line hDUB4, 5 hOOB4.8 hDOB8.2 hDUB4.5 hDCJB4-8 HDUB8.2 ΜΙ^ΡΑΚΗίΚΤΙ^ΒΟΙΑδΡΟΝΙ^βΟΟΚΝΙ^νη^νΡνΡΜΕΞ^ΥΙ^ΟΟΕν^ΓΝΗΕ^Τε^Ρ 60 MRQRAKHLKTLSEGXASCCKLRSQQKNLVILVPVPMEDD5LYLGGEWQFNHFSKLTSSRP 60 MRPESPSFED-SSEIASFCNLRSQPKNLVILVPGDMEDDSLYLGGEWQFKHFSKLTSSRP 59 *# , . * * * * * 食》 ****·*««* ♦«食*** will eat the word «flank* # DAAFAEIQRTSLPEKSPL SCETRVDLCDDtAPVARQLAPREKLPLSNHRPAAVGAGLQNM 12 0 ΟΑΑΚΑΕΙΟΚΤΒΙιΡΕΚΒΡΙ^ΕΤΗνΟΣΧΠΧΪΙΑΡνΑΚΟΕΑΡΚΕίαΡΙ^ΚΚΡΑΑνσΑΟΙίΟΝΜ 12 0 ΟΑΑΚΑΕΙΟΙΙΤΒΙϋεΕΚεΒΙ^ΕΤΙΐνΌΙ^ΟϋΟΙΑΡνΑΙΙΟΙΑΡΚΕΚΙ^ΙιεεΜΙΡΑΑνΟΑΟ^ςίΜ 119 »*胁俞**«««会《* *** Yu# 贪♦食* # » * * 舍♦ * ·* « 会★« « ♦« *#« Poultry and poultry #-*«*'**#**·*** This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. Description of the invention (82 hDUB4.5 h〇UB4.8 hDUB8.2 hDUB4.5 hOUB4.8 hDUB8.2 10 hDOB4.5 HDUB4.8 hDUB8.2 15 hOUB4.5 hDUB4.8 hDUB8.2 20 h〇UB4 .5 hDUB4.8 hOUBS.2 25 hDUB4.5 hDUB4.8 h £ &gt; 0 &amp;. 8.2 hOUB4.5 hOUB4.8 hDUBS.2 35 hDUB4.5 hDDB4.8 hDUB8.2 GNTCYVNASLQCLTYTPPLANYMLSRSHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQP 180 GNTCYVNASLQCLTYTPPLANYMLSREHSQTCHRHKGCMLCTMQAHITRALHNPGHVIQP 180 GNTCYVNASLQCLTYTPPLANYMLSRBHSQTCHRHKCCMLCTMQAHITWPLHSPGHVIQP 179 SQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKI &gt;TTLIHQrFGGYWRSQI 240 SQALAAGFHRGKQEDAHEFLMFTVDAMKKACLPGHKQVDHHSKDTTLIHQIFGGYWRSQI 240 SQVLAAGFHHGEQEDAHEFLMFTVDAMKKAPLPGHKHLDNHSKDTTLIHQIFGGYWRSHI 239 Threat* ♦**#*«*食=*夤'*««««亩》**«««««« λ#«**·:«·«** Α*食*♦ 贪食 “*·*«, greedy KCLHCHGISDTFDPYLDIALDIQAAQSVQOALEQLVKPEELNGENAYHCGVCLQRAPASK 3 0. KCLHCHGISDTFDPYLDIALDXQAAQSVQQALEQLVKPEELNGENAYHCGVCLQRAPASK 30Q NCFHCHGISDTFDPYLDIALDIQAAQSVKQAL----------------------------271 ·»·»*»#**·«»«★«·*# ** «« ** 黉 * threat: Cao · # TLTLHTSAKVLILVLKRFSDVTGNKIDKNVQYPECLDMKLYMSQTNSGPLVYVLYAVLVH 360 TLTLHTSAKVLILVLKRFSDVTGNKIAKNVQYPBCX, DMgPYMSQQPrrGPLVYVl.YAVLVH 360 AGWSCHNGHYFSΥνΚΑΟΕσΟν/ΥΚΜΌΟΑΒνΤΑεβΙΤενί^ΟΟΑΥνί^ΚΥΙΟΚΒΕνίΕΚΙ^ΕΒν 4 2. AGWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASITSVLSQQAYVLFYIQKSEWERHSESV 420 fiRGREPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQESTLDHWKFLQEQNK 4β0 SRGRBPRALGAEDTDRRATQGELKRDHPCLQAPELDEHLVERATQBSTUJHWKFLQEQNK 4 80 ΤΚΡΕΓΝνΚΚνΒσΤΙιΡΡΙϊνΐ / νΐΗΟδΚΥΙ ^ ΜΚΝΗΗΡΕΟΟββΙΛΜ ^ ΤΤΡΤΗζ ^ ΕΗΜΝΤΟΊΧ 540 TKPEFNVRKVBGTLPPDVLVIHQSK ^ KCGMKNHHPEQQSSLLNLSSSTPTHQESMNTGTL 540 'ASLRGKARRSKGKNKHSKRALLVCQWSQHKYRPT 574 ASLRGKARKSKGKKKHSKRALLVCQ --------- S65 40 45 Table 27. hDl) B4.5, hDUB4 ^ and hDUB8 .2 The initial iodine nucleotide sequence pair (presumed promoter region) is upstream (number starting from the starting ATG) hDOB4.5 hDUB4.8 hDUB8.2 50 lS 1.8 HDDB4. hDDB4. hDUB8.2 55 CACACGAACACAATCACACACACACTCACACGGTTTCCTACGTAAAGATTTCTTCCCT -276 CACACGAAjCAC^TCACACACACACACTCACACGGTTTCCTACGTAAAGATTTCTTCCCT -276 GGGAiSAAAAACACACACACACACACACACACACGGTTTCATAGGTAAAGArTTCTTCCCT -27 6 Meetings*# ♦ will ♦ «会***会会会会* * 亩亩会会会会会《禽《*« «俞食食会*« *♦*♦«*#«# will GCCATTGCTTTACCTAAAATAAGGCAA CTGTGTGGCCACTGTCCCAACCCGGTTACACTC -216 GCCATTGCTTTACCTAAAATAAGGACTGTGTGGCCACTGTCCXAACCCGGTTACACTC -216 GACATTGTTTTACCTiUUATAAGGaU^TGTCTrGGCCACrGTCCCAftCCCGGTTACACTC -216 Yu**#«♦«»*«♦***#♦##***会♦*会金》★***俞#*会*«»* «食仓仓*食" «俞《会**会喻*♦ Yushe Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 60 65 hOUB4.5 h〇UB4.8 hOUB8.2 hDUB4.5 hDUB4.8 hDDB8. 2 CTATTATATGTaCCTATCATCCTGAGGAGTAATTTGATTCAGGTGTTCTGGilAGTCATGC -156 CTATTATATGTGCCTATCATCCTGAGGAGTAATTTCMTCAGGTGTTCrGGAAGTCATGC -156 ATATTACATGTGTCTATCAGCCTGAGGAGTAGTTTGATTCAGGTGTTCTAGAAGTCATGA -156 · **** «# * * * * ******« threatened homes ♦ gold * * * * << << food "threat will * be * · * # TGTQGGCTOrGTCTGrrGAATTCCCAaCGATGCAAGQGGACACACCCTGTGACTCCTTCC - 96 TGTGGGCTGTGTCTGTTGAATACCCAGCGATGCAAGGGGACACACCCTGTGftCTCCTTCC -96 TGTGGGC^TQTCTGTTGAATTCCCAGCGATGCAAGOGGACACACCCTGTGACTCATTCC -96 ★ ♦♦♦*«**♦«**♦♦**»♦#♦« ♦«*♦«***♦♦****♦ ♦*★♦ ★***#*'**««««♦ ««♦ will hCOB4.S TGAA TTGAGTGCTGATXTTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGGTGTTC -36 •84- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (83 hOUB4.8 hOUB8.2 TGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGGTGTTC - 36 TTAATTGAGTGCTGATATTTGATTGGTTTATCGCGCACCTGATGGGTGGGTGGGGTGTTC -36 hDUB4.5 hDUB4.8 hDUB8.2 cscggttggtggggttgacttacagaagggctgatg GCGGTTGGTGGGGGTGACTTACAGAAGGGCTGATG gcggttggtgggggtgagttatataagggctgatg *mu ♦ * (·食肯* « 会会会资卖会* 賨会会«««背*会表 28. hDUBs core amine The CLUSTAL W (i.8丨) multiple sequence 棑 ratio of the base acid is 8. 5 8. 7 15 8. 1 4 . 2 4 · 4. i 4. 1 20 8. 3 β. 11 8. s 8. 6 25 8 5 8 1 8 1 4 2 30 4 3 4 4 5 1 35 8 8 u β 8 6 S 5 40 8 7 a 1 4 2 4 3 4 5 45 4.1 8.3 6 11 8 Θ β 6 50 8 5 SQ 7 1 55 P 4 X 2 4 3 4 5 4 1 8 3 60 8 11 S 8 8 6 MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSEARVDLCDDLAPVAR 60 MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSSEARVDLCDDLAPVAR 60 ΜΟΟΟεί / ΥΙΛαΕϊίΟΓΝΗΓΞΚΕ / ^ ΚΡΟΑΑΚΑΕΙΟΚΊ ^ ΡΕΚβΡΙ ^ ΕτκνΟΙ ^ ΟΙίϋΙΑΡνΑΚ 60 MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVD3XDDLAPVAJI 60 MEDDSLYLGGEWQFNHFSKLTSSRPDAAFAEIQRTSLPEKSPLSCETRVDLCDDLAPVAR 60 MEBDSLyLGGEWQFNHFSKLTSSRFDAAPAEIQRTSLFEXSPLSCETRVDLCDDLAFVAR 60 meddslylggewqfnhpskltssrpdaafaeiqrtslpeksplscetrvdlcddlapvar 60 MEDDSLYLGGEWQFNSPSKLTSSRPDAAFAEIQRTSLPEKSQLSTETRVDFCDDLAPVAR 60 MEDDSLYLGGEWQFNHFSKLTSSRPDAA7AEIQRTSLPEKSQLSTETRVDFCDDLAAVAR 60 MEDDSLYLGGDWQFNHFSKLTSSRLDAAFAEIQRTSLSEKSPLSSETRFDLC33DLAPVAR 60 MEDDSLYLGGDWQFNHFSKLTSSRLDAAPAEIQRTSLSEKSPLSSETRFDLCDDLAPVAR 60 *, # ♦ food • will * # * * # * << fee * ♦ * «*#* * Threat ♦*«**« 会# Threatened by Yu Wen* * * * . * Food *«*** ***

QLAPRKKLPLSSRKPAAVGAGLQNMGNTCYENASLQCIiTYTPPLANYHLSRSHSQTCQRF 120 QLAPRKKLPLSSRRPAAVGAGLQNMGNTCYENASLQCLTYTPPLANYMLSREHSQTCQRP

SREHSQTCQRP SREKSQTCHRH SR2HSQTCHRH SRSHSQTCHKH 120 120 120 120 120 120 billion £ * ΆΡΚ £ ΖαΡΙι55ΚΚΡΑΑναΑ01 «ςΝΗ5ΚΤΟΥεΝΑ £ Ι &lt; 〇ΟίΤΥΤΙ * ΡΙ &lt; ΑΝΥΜΖ ^: ΟΙΛΡΚΕΚΧίΡΙ ^ ΚΚΡΑΑνΟΑσί ^ ΝΜΟΝτΟΥνΝΑΒυοσνΤΥΤΤΡΓΑΝΥΜΙ ^: QLAPR £ IU &lt; PL5SRRPAAVGAGI ^ MMGNTCYVIIASLQCX« TYTPFLANYMLSRSHS &lt; QLAPREKLPLSNRKPAAVGAGLQNMGNTCyVNASLQCLTYTPPLANYMLSREHS &lt; QLAPREKPPLSSRRPAAVGAGLQNMGNTCYVNASLQCLTYKPPLANYMLPREHSQTCHRH QU ^R£KLPL5SRRPAAVGAGI^2WNTCYVNASQQCLTYTPPLANYHLSR£HSQTCHRH 120 QIAPRBKLPLS5RRPAAVGAGLQNHSHTCYVNASQQC1»TYIPPLANYMLSRSHSQTCHRH 120 〇£ιΑ£&gt;Κ£ΙΟ.ΡΙ«55ΚΚΡΑΑν〇Α〇Ιι〇ΚΙΟΝΤΡΥνΝν8Ιι〇α«ΤΥ,Π.ΡΙι5ΝΥΜΖ*5Κε〇8〇Ταΐυ{ 120 〇ΙΑΡΚΕΚΙ ····································食*俞" ♦*·**** ♦« ** Food · ΙΧΟΐΚΓΓΗΟ ΗΙΤΗΑυ ^ ΡΟΗνίςΡΣΟΑίΑΑαΡΗΗβΚΟΕηΑΗεΐΊιΜΡΤνΟΑΜΚΚΑΐΧΡΰΗ 180 KCCMLCTMQAHITWAl ^ SPGHVIQPSQJU ^ AAGFHRGICQEDAHEFLMFTVDAMKKACLPGH 180 KCCKLCTUQAHITWAZiHSFGHVIQPSOALAAGFHRGKQEDVHEFLMFTVDAMKKACLPGH 180 Κ6〇Η1ΧηΜ〇ΑΗΙΤΚΑ1 1ΝΡΟΗνΐ〇Ρ8〇ΑΙΑΑΟΡΗΚ6Κ〇εΐ2ΑΗΕΡΕΜΡΤνΏΑΜΚΚΑ ^ ^ ΡΟΗ 180 KGCHLCTNQAH station TRALHNPGHVIQPSOALAAGFHRGKQEDftHEFLHPTVDAMKKACLPGH ΙβΟ KGCHLCTMQAHITRAUINPGHVIQPSQALAAGFHRGKQBDAHEFLHFTVDAMKKACLFGK 180 ΚΟ € ^ ε ^ ΑΗΧΤΚΑ &amp; ^ υ ^ ΟΡΗΚΟΚ ΗΙΡ6Ηνΐ〇Ρ3〇ΑΙ 〇ε〇ΆΗΕΡΙ ^ (ΡΤ ^ ΏΑΗΚΧΑα.Ρ6Η 180 KCCH1XTH £ AHITWP1 £ IFGHVIQPSQALAAGFHRGKQEAALEFLMFTVI) SAMKICACLPGH 180 KCCMLCTMEAHITWPUIIFGHVIQPSQALAAGFHRGKQEAALEFLMFTVDAHKKACLPGH 180 KCCHFCTMQAH station TWALYRPGKVIQPSQVLAAGFKRGHQEDAHEFLMPTVDAMKKACLPGH 180 KCCHFCTMQAMITWALYRPGHVIQPSQVIAAGFHRGEQEDAHBPLHPTVDAKKKACLPGH 180 Κζ ^ νϋΗΗεΚϋΤΤΙ ^ ΗςίΓΟζκ ^ ΗβςίΚζ ^ ΗΟϊσίεΟΤΡΟΡΥΙΛΙΑ ^ ΟΙΟΑΑΟδνΚΟΑΙ ^ Ε ΟΙ * 240 KQVDHHSKDTTLIHQIFGGCWRSQIKCliHCHGISDTFDPYLDIALDIQAAQSVKQALEQL 240 KQVDHHCKDTTLIHQIFGGCWRSQIKCLHCHGISDTPDPYLDIALDIQAAQSVKQALEQL 240 ΚΟνΟΗΗεΚϋΤΤΙ ^ ΙΗςίΓΟσΥΗΚβΟΙΚα / ΙΚΗσίΒΟΤΡΟΡΥΙιΟΙΑωϊΙΟΑΑαδνΟΟΑυΕζ}! * 2Λ0 KQVDHHSKDTTLIH0IFGGYWRSQIKCLHCHGISDTPOPYLDIALDIQAAQSVQQALEQL 240 KQVDHHSKDTTLIHQIFGGYWKSQIKCIiHCHGISDTFDPYLDIALDIOAAQSVQQALEQL 240 KQVDRHSKDTTLIHQIFGGWRSQIKCLHCHGISDTPDPYLDIALDIQAAQSVQQALEQL 240 KQVDHHSKDTTLIHQIFGGYWRSQIKCLHCHGISDTFGPYLDIALDIQEAQSVKQALEQL · 240 K ---------------------- QILILVWKRFSDVTG- ---------------------196 Κςΐ^Η^ΚΟτΊΧΙΗςίΓΌΑΥνί^ΙΚΥΙίίΚίΗαΐΒΟΤΡΙΪΡΥυ)工ALDIQAAQSVKQALEQL· 240 KQLDHHSKDTTLIHQIFGAWRSQIKYLHCHGISDTFDPYLDIALDXQAAQSVKQAI.EQL 240 -85- This paper scale applies to Chinese national standards (CNS A4 size (210 X 297 mm) 1312008 A7 B7 V. Description of invention (84 10 15 20 25 30 35 40 45 50 1 2 3 5 1 3 11 8 6 8.5 8.7 8 4 4 4 4 β 8 β 8 2 3 5 1 3 XI 8 6 1 23 5 X 3 U 8 6 8.S 8 .7 8 4 4 4 4 Β β 3 118 6 νΚΡΕΕΙ ^ σΕΝΑΥΗΟσί ^ Ι ^ ΚΑΡνβΚΤί ^ ΗΊΤΑΚΕΚΙΙ ^ ΕΤΟΚΡνΛ / νΤΚΗίαΑ ^ νΟΥΆΕβ 300 VKPEELNGENAYHCGLCLQRAPASKTLTLHTSAKVl.ILVLKRFSDVTGiiKLAKNVQYPEC 300 VKPEELNGBNAYHCGLCLQRAPASNTLTLHTSAKVLILVLKRFSDVAGNKLAKNVQYPEC 300 VKPEELNGENAYHSGVCLQRAPASKTLTLHTSAKVLXLVLKRFSDVTGNKIAKNVQYPEC 300 VKFEELNGENAYHCGVCLQRAPASJCTlrTLHTSAXVLILVLltRPSDVTGNiaAKm / QYPEC 300 VKPEELNGENAYHCGVCLQRAPASKTLTLHTSAKVL station LVLKRFSDVTGMKIDiCNVQYPEC 300 VKPEEIJiGENAYHCGVCLQRAPASKTLTLHNSAK''7LILVLKKFPDVTGNKIAKWVQYPEC 300 VKPEELNGENAYHC ---------------------------------GNKIAKNVQYPEC 267 --------------- ---------------------------------NKIAKNVQYPEC 208 VKPKELNGENAYHCGLCLQKAPASKTLTLPTSAKVLWorks/νΐ^ΚΚΕ^ΟνταΝΚΙΑΚΝνΟΥΡΚ. 300 νΚΡΚΕυ ^ ΕΝΑΥΗΟαί ^ ΙΟΚΑΡΑεΚΤΙ ^ ΡΤεΑΚνί ^ νΐ ^ ΚΗΡεΟνΤΟΝΚΙΑΚΝνίίΥΡΚσ 3 00 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEGQWYKMDDAKVTACS IT 3 6 0 LDMQPYMSQQNTGPLVYVLYAVI / VKAGWSCHDGHYFSY ^ / KAQEGQWYKMDDAKVTACSIT 360 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHDGHYFSYVKAQEVQWYKMDDAEVTVCSII 360 ΙίυΜ〇ΡΥΜ3〇ςΝΤσΡΓΛ / Ύνΐ »ΥΑνΐ (νΗΑίΏί50ΗΜ3ΗΥΚ3ΥνΚΑ〇Εσ〇» ΥΚΗηΠΑΕνΤΑΑ5; ΓΊ '360 LDMQPYMSQQNTGPLVyVLYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTAASrr 3SO LDKKLYMSQTNSGPLVYVLYAVLVHAGWSCHNGHYFSYVXAQEGQWYKMDDAEVTASSIT 360 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCMJflGHYSSYVKAQEGQWYKMDnAEVTASSIT 360 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKVQEGQWYKMDDAEVTASGIT 3 2 7 LDMQPYMSQQNTGPLVYVLYAVLVHAGWSCHNGHYFSYVKVQEGQWYKjMDDAE ------- 261 rGPLVYVLVAVLVHAGWSCKNGHYFSYVKAQEGQWYXMDDAEVTASGIT 360 LYAVLVHAGWSCHNGHYFSYVKAQEGQWYKMDDAEVTASGIT 360 »*****« **** '*. *** # * ** # · «***. RDMQPYMSQQNTGPLVYVL'ii κι»ι〇ρΥΜ3〇〇Ντσρχ,νγ^ν * * . * * * * «♦ greedy birds threatened birds Cong Mu Yu fees SVLSQQAYVLFYIQKSEWERiiSESVSRGHEPRALGAEDTDRKATQGELKRDHPCLQAPEL 420 SVLSQQAYVLFYIQKSEWEHHSESVSRGREPRALGAEDTDRRATQGELKRDHPCI ^ QAPBL 420 SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRAKQGELKRDHPCLQAPEI, 420 SALSQQAYVLFYXQKSEWERHSESVSRGREPRALGTEDTDRRATQGELKRDHPCLQAPEL 420 SVLSQQAYVLFYIQKSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDHPCLQAPEL 420 βνΧ ^ ΟςΑΥνΐ ^ ΎΙΟΚΒΕΜΕΗΗβΒΗνεϊΙΟΚΕΡΚΑΙΛΑΕΟΤηΚΚΑΤΟσΕΙ / ΚΚΒΗΡΟΙ ^ ΑΡΕίι 420 SVLSQQArVLFYIQKSEWERHSESVSRGREPRALGVEDTDRRATQGELKRDHPCLQAPEL 42 0 SVtSQQAYVLFYIHKSEWERHSBSVSRGREPRALGAEDTDRRATQGELKRDYPCLQVPEL 387 --------- ----- KSEWERHSESVSRGREPRALGAEDTDRRATQGELKRDYPCLQVPEL 307 SVLSQQAYVLFYIQKSEWERHSESVSRGREPBALGAEDTDRPATQGELKRDHPCLQVPEL 420 SVLSQQAYVLFYIQKSEWERHSBSVSRGRSPRALGAEDTDRPATQGELKRDHPCLQVPEL 420 acres acres Lun ★ gold greed # * # Weng Yu Jin will hon greedy greedy * # Qian ★ poultry food * # * DERLVERATQESTIiDHWRFPQEQNKTKPEFNVRKVBGTLPPMVLVIHQSKYKCGMKNHHP 480 DERLVERATQESTLDHWRFPQEQNKTKPEraVRKVEGTLPPNVLVIHQSKYKCG MKNHHP 480 DEHZ, VERATQESTLDHWKFLQEQNKTKPEPNVGKVEGTLPPNALVIHQSKYKCt3MKNHHP 480 DEHLVEHATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHP 480 DEHLVERATgESTLDRWKFLQEQNKTKPEFHVRKVBGTLPPDVLVIHQSKYKCiSMKNHHP 480 DEHLVERATQESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHP 480 DEHLVERATQESTLDHWKFXQEQNKTKPEFNVRRVEGTVPPDVLVIHQSKYKCRMKNHHP 480 DEHLVERATQESTLDHWKFLQE ^ JKTKPEFNVRKLEGTLPPNVLVIHQSKYKCGMKNHHP 447 DEHLVERATQESTLDKWKFLQEQNKTKPEFNVRKLEGTLPPNVLVIHQSKYKCGMKNHHP 367 ΟΕΗΙ * νΕΗΑΤΌΕ5Τυ »η &lt; ΚΡΡςΚ (3ΝΚΤΚΡΕΕ · ΝνΗΚνΕΟΤΙίΡΡΝνΐΛ / ΙΗ (35ΚΥϊα: (3ΜΚΝΗΗΡ 480 PNVL 'DEHL, -VERATQBSTLDHWKFPQKQNKTKPEFlfVRKVEGTLPPN will eat food with a short-tailed" «#会负«•禽•费*.********** ·*贪***# VIHQSKYKCGMKNHHP 480 #·*贪***** 丨金«* threats Ministry of Economic Affairs Intellectual Property Bureau Employee consumption cooperatives print 6 8 paper scales applicable to China National Standard (CNS) A4 specifications (210 X 297 mm) 1312008 A7 B7 V. Invention description (85 10 8.5 8.7 8 4 4 4 4 8 8 8 2 3 5 1 3 11 3 8.6 EQQSSLLNLSST TRTDQESVNTGTLASLQGRTRRSKGKNKHSKRALLVCQ 530 EQQSSLLNLSSTTRTDQBSVm'GTLASLQGRTRRSKGKinCHSKRALLVCQ 530 ΕΟςβΞΙΧΝΙ^ΤΤΙΙΤΠΟΚΜΝτΟΤΙ^Ι^ΟσΚΤΚΚΑΐαϊΚΝΚΗδΚΚΑΙ^ν. . 5 3 0 EQQSSLLNLSSSTPTHQESMNTGTLASLRGRARHSKGKNKHSKRALLVCQ 530 EQQSSLLNLSSSTPTHQESMNTGTLASX, RGRARRSKGKNKHSKRALLVCQ 530 EQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ 530 EQQSSLLNLSSTTPTDQESMNTGTLASUIGRTRRSKGKNKHSKRALLVCQ 530 EQQSSLLNLSSTNPTDQESMNTGTLASLQGRTRRAKGKNKHCKRALLVCQ 497 ΕζΧ ^ Ι ^ υίΙ ^ ΤΝΡΤΟΟΕεΜΝΤσΤΙΑβΙ ^ ΟσΚΤΚΐ ^ ΚσΚΝΚΗΟΚΚΑΙΧνας 417 EQQSSVLNLSSTK ΡΤΓ) 〇Ε5ΜΝΤσΤΙ * Α5Ι ^ 03ΤΗΚ3ΚΟΝΝΚΗ3ΚΚ3 ^ greedy ν〇ς 530 EQQSSLLNLSSTKPTDQESMNTGTLASLQGSTRRSKGNNKHSKRSLLVCQ 530 * ** ★: *惫, *, ★★*·***·* Food **♦·# ·★♦«* threat-corruption ♦ ♦ Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing -87- This paper scale applies to Chinese national standards ( CNS) A4 size (210 x 297 mm) A7 B7 1312008 V. INSTRUCTIONS (86) Table 29. CLUSTAL W (L81) multiple sequence row tb (upstream of ATG start codon) for hDUBs putative initiation sequence 8 · 9 TGACGTGTGTGAAAACTACAGTGTGATGAGCATGACTTGC AG ACAGGTTATCGATTGGGC 6 0 5 8.10 -GACGTGTGTGAAAACTACAGTGTGACGAGCATGACTCGCAGACAGGT TATCGATTGGGC 59 g 2 — — · ~ - · ^ -a . . . — — — — — —------GATATC^^^TACGGC 14 Θ.11 -------------- ---------*----------------------GATATCAATACGGC 14 4.2_a TTATCGATTGGGC 13 4.5~ TGAGCATGACTGGCAGACAGCTTATCGATTGGGC 34 10 4.3 GATGAGCATGACTGGCAGACAGCTTATCGATTGGGC 36 4.2_b GTGATGAGCATGACTGGCAGACAGCTTATCGATTCSGGC 3 8 4.4_ GTGATGAGCATGACTGGCAGACAGCTTATCGATTGGGC 38 4, X_b AGTGCAATGAGCATGACACGCAGGCAGGATATCAATTCGGC 41 8.1 A CAATGAGCATGACACGAAGACAGAATATCAATTCGGC 37 15 8.7 CAATGAGCATGACATGCAGGCAGGACATCAATTCGGC 37 8.6 ---------------------TGTGATGAGCATGACTCGCAGACAGGTTATCGATTGGGC 39 8.8 ------- --------------TGTGATGAGCATGACTCGCAGACAGGTTATCGATTGGGC 39 8.5 -----------------TCTCTGTCAGAACCATGGTACTCTGTTGTGGTGTGAAAGTAGC 43 8.2 TCACATCTTCTCCXKXAGATGCAGCCTTTGCTGAAATCCAGCGGACTTCTC 51 2〇4.1一 a AGTATAC5CAGAGCAGAGAGCTG-GAAGGGACC 31 * * 3.9 T-CCCCTCAAAAT-TAGTTATGAGCATTAAAGGACACCGATGCCC——AGGTCCCGGCTG 115 8.10 T-CCCCTCAAAAT-CAGTTATGAGCATTAAAGCACACCGATGCCC —-AGGTCCCGGCTC 114 25 8.3 T-CACCTCA AAAG-CAGTTATGAGCATTAAAGGACACCCATGCCT- - - AGGTCCCGCTTA 6 9 8.11 T-CACCTCAAAAG-CAGTTATGAGCATTAAAGGACACCCATGCCT - AGGTCCCGGTTA 69 4.2 a a T-CCCCTCAAAAT-CGGTTATGAGCATTCAAGCACACCGATGCCC --- AGGTCCCGGCTG 68 4.5 T-CCCCTCAAAAT-CGGTTATC3AGCATTCAAGCACACCGATGCCC --- AGGTCCCGGCTG 89 4.3 T-CCCCTCAAAAT-CGGTTATGAGCATTCAAGCACACCGATGCCC- --AGGTCCCGGCTQ 91 30 4 · 2 a b T-CCCCTCAAAAT-CGGTTATGAOCATTCAAGCACACCGATGCCC-AGGTCCCGGCTG 93 4.4 T-CCCCTCAAAAT-CGGTTATGAGTATTCAAGCACACCGATGCCC --- AGGTCCCGGCTG 93 4 _ l_b T-CCCCTCAAAAG-CTGTTATGAGCATTAAAGGACACCAATGCCT-AGTTCCCGGTTA 96 8.1 T-CACCTCAAAT - CAGTTGTGAGCATTAAAGAAAACCAATTCCT-AGGTCCCGCTTA 91 8.7 T-CACCTCAAAAG-C ^ TTATGAGCATTAAAGGACAACAATTCCT --- AGGTCCCGCTTA 92 35 8.6 T-CCCCTCAAAAT-CiW5TTAGGAACATC3AAAGCACACCGAtGCCC --- AGGTCCTGGCTG 94 8.8 T-CCCCTCAAAAT-CAGTTAGGAACATGAAAGCACACCGAt &lt; 3CCC --- AGGTCCTGGCTG 94 8.5 CACAGATCATCTG-TAGAT-TAAGGGGTCTGGCTTTGTTCCAACA - AAGCTTTATTTA 98 8.2 TCCCTGAGAAGTCACAACTCTCAACTGAGACCCGCGTCGACTTCTGCGATGATTTGGCGC 111 4.1_a TGCATCCCTAAT---- GATATAAGAAAGTATCTGTACTAGCCCTGA-ATGGTATAACTA 85 40 * * * * 8.9 CAGGAATAAGAC -CCTCCGACGTCTTGTGTGAAGCCACGGC - ATCTTCGATTGCTCATGC 172 8.10 CAi3GAATAAGiW: -CCTCCAGCGTCTTGTCTGAAGCCACGGC- * ATGTGGATTGCTCATGC 171 8.3 AAGAGATAAGAC-TCTCCCACACCCTGTGTGAAGCCACGGC - ATGTGGATTGCTCATGC 126 45 8.11 AAGAGATAAGAC-TCTCCCACACCCTGTGTGAAGCCACGGC-ATGTCGATTCCTCATGC 126 4.2_a CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC - ATCTGCATTGCTCATGC 125 4.5 CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC - ATCTGCATTGCTCATGC 146 4.3 CAOTAATAAGAC-CCTCCAGCXm: TTGTGTGAAGCCTCGGC - ATCTGCATTGCTCATGC 148 4.2_b CAGGAATAAGAC-CCTCOM3GGTCTTGTGTGAAGCCTCGGC - ATCTGCATTGCTCATGC 150 50 4.4 CAGGAATAAGAC-CCTCCAGGGTCTTGTGTGAAGCCTCGGC - ATCTGCATTGCTCATGC 150 4. l_b AAACGATAAGAC-TCTCKCACACCCTGTGGGAAGCCACGGC - ATCTGGArTGCTCATGC 153 8-1 AAGAGATAAGAC-CATCCAACAACCTGTG*rGAAGCCACCGC--ATCTCCXrrTGCTCATGA 148 8.7 AAGAGATAAGAC -CATCCAACACCCTGTGTGAftGCCACGGC--ATCTGGATTGCTCATGT 149 Ministry of Economic Affairs Intellectual Property Office Staff Consumer Cooperative Printed 8.6 CA&lt;K3AATAAQAT-CCTCCGACGTC TTGTGTGAAGCCACGAC - ATCTGCATTGCTCATGC 151 55 8.8 CAGGAATAAGAT-CCTC05ACGTCTTGTGTGAAGCCACGAC - ATCTGCATTGCTCATGC 151 8.5 CAAACACAGGCTGTGGGCTGGATTTGGCCTGCAGCTCTAGT - TTGTG ---- ATCCTTGA 152 8.2 CTGTGGCAAGAC - AGCTTGCTCCXAGGGAGAAGCTTCCTCTGAGTAGCAGGAGACCTGC 169 4 · 1 a a CAG-GTTAAATT --- TACGTGAAAAAGAAATCAiWrrrCTGCCTTGTTTAAGCAAACTTA- 140 60

This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm). 1312008 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (87) 8 9 8 10 8 3 8 11 5 4 2_a 4 5 4 3 4 2_b 4 4 10 4 l_b 8 1 8 7 8 6 8 8 15 Θ 5 a 2 4 l_a 20 8 9 8 10 3 3 3 11 4 2_a 25 4 5 4 3 4 2_b 4 4 4 1-b 30 8 1 8 7 3 6 8 8 8 5 35 8 2 4 l_a 8 9 40 8 IQ 8 3 8 11 4 2 One a 4 5 45 4 3 4 2 One b 4 4 4 lb 8 1 50 8 7 8 6 8 3 8 5 8 2 55 4 l_a 228 227 182 182 181 202 204 tTCTG-G-GGATCATTCTCCTGAAAATG - GTGGCTCCTTCCTGCCTGTGGAGCACCTCT tTCTG-G-GGATCATTCTCCTGAAAACG- -GTGGCTCCTTTCTCCCTGTGGAGCACCTTT tTCTG-G-GGATCATTCTCCTGAAAATG - GTGGCTCCTTTCTCACTGTGGAGCATCTTT ttctg-g-ggatcattctcctgaaaatg - gtggctcctttctcactgtggagcatcttt tTCTG-G-GGATCATTCTCCTGAAAATG --GTGGCTCCTTTCTCCCTGTGGAGCATCTTT TTCTG -G -GGATCATrrCTCCTGAAAATG--GTGGCTCCTTTCTCCGTGTGGAGCATCTTT TTCTG -G-GGATCATTCTCCTGAAAATG - -GTGGCTCCTTTCTCCGTGTGGAGCATCTTT TTCTG-G-GGATCATT CTCCTGAAAATG-GTGGCTCCTTTCTCCCTGTGGAGCATCTTT 206 TTCTG-G-GGATCATTCTCCTGAAAATG - GTGGCTCCTTTCTCCCTGTGGAGCATCTTT 206 3-G-GGATCATTTTCCTGAAAATC-GTGGfCTGCTTTCTCCCTGTGTAGCATCTTT 209 -GGATCATTCTCCAGAAAATG - GTGGCTCCTTTCTCCCTGTGGAGCATCTTT 204 TTCTG-G-GGAACATTCTTCTGAAAATGGCGGCTCCTTTCTCCCTGTGGAGCATCTTT 205 TTCTG-G-GGATCATTCTCCTGAAAATG-GTGGCTTCTTTCTCCCTGTGGAGCATCTTT 207 TTCTG-G-GGATCATTCTCCTGAAAATG - GTGGCTTCTTTCTCCCTGTGGAGCATCTTT TTCAG -ACAGTTTAGCAAGGCTGAAAAG--AACACCCACACCCCCTTGTTACCCACAGAT TGCGGTGGGGGCTGGGCTCCAGAATATG- -GGAAATACCTGCTACGTG---AACC5CTTCC 3——GCATTAATTTTATAAATATGTAGAGAATACATACTCCTTAT-GAGCAGA---

TTCTG-GG TTCTG-GG rTCAG- * * * 207 209 224 193 SAAAGCCTTCT 285 cta-agca-gtgc-cctttcttcacccaggacactttacatcaggcacagaaagccttct 234 GTA-AGCA-GTGT-CCTTTCTTCCCCCAGGACACTTTACTTCAGGCACAGGAAGCCTTCT 239 CTA-AGCA-GTGT-CCTTTCTTCCCCCAGGACACTTTACTTCAGGCACAGGAAGCCTTCT 239 CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT 23 9 CTA- AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT 260 CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTrTACATCCGGCACAGGAAGCCTTCT 262 CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGOAAGCCTTCT 264 CTA-AGCA-GTGCTCTTTTCTTCCCCCAGGACACTTTACATCCGGCACAGGAAGCCTTCT 264 CTA-AGCA-GTGCTCCTTTCTTCCCACAGGAAACTTTACATCAC3GCACAGGAAGCTTTCT 2 67 CTA-AGCA-GTGC-CCTTTCTTCCCCCAGGACACTTTACATGAGGTGCAGGAAGCCTTCT 261 CTA-AGCA-GTGCTCCTTTCTTCCCCOW3GACACTTTACATCAGGCATAGGAAGCCTTCT 263 CTA-AGCA-GTGCTCCTTTCTTCCCCCAGGACACTTTACATCAGGCGCACGAAGCCTTCT 265 CTA -AGCA-GTGCTCCTTTCTTCCCCCAi^ACACTTTACATCAGGCGCACGAAGCCTTCT 26&amp; gggtggga-ctgtgttggccagagaccgagagacgggtoctcacaggggaacgtacagca 268 CAGCAGTGTCTGACATACA CACCGCCCCTTGCCAACTACATGCTGTCCCGGGAGCACTCT 284 -AACAATGrrTGCGCCATATGGTCCATGATGGGTGTTCAATAATGTGTGATGATGATAAT 252 CTA-AGCA-GTGC -CCTrTCTTCACCCAGGACACTTTACATCAGGCfl

GATGGAGCACACCTGGCCCATGAAAAGACAAGGGATAAGAAACGCX3GCXAAACATCACAG 345 gatggagcacacctggcccatgaaaaaacaaggga-aagaaacggggccaaaggtcacag 343 GATGGAOCACACCTGGCCCATGAAAAGACAAGGGA

CCTGGCC gatggagcacacctggcccatgaaaagacaaggga-λ GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-ii GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-Λ GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAACSGTCACAG 323 GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAATGGOGCCAAAOGTCACAC 326 SAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAAAAGOGCCAAAGGTCACAG 320 .GTACACCTGGCCCATGAAAAGACAAAGGA-AAGAAACAGGGCCAAAGGTCACAG 322 GATGGAGCACACCTGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG 324 GATGGAGCACACCrGGCCCATGAAAAGACAAGGGA-AAGAAACGGGGCCAAAGGTCACAG 324 TGTAGAGGCCGGAAGGTGCTCCAGGGCACAAGTGT-GGGAAAGTGGGACATACGGGGAAG 327 CAAACATGTCATC-MSTCACAAGTGCTGCATGCTCTGTACCATGGAAGCTCACATCACAT 342 AATGAAGACAATAGTGACAAATAAAAGAAAATAA ^ 'AAGCAGTGAAACAAAGTGGTTTAA 311 GATGGA &lt; GATGAA, -89- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. Invention description (88) 9 10 3 11 2 A a 2_b 4 b 15 20 25 30 35 45 50 6 8 5 2 1 a a 9 10 3 11 2_a 5 32Jb 4l_b 2 i_a 10 311 2_a 5 3 2 a b 4 1-b

TCCTCTCATTCCACCGTCCTCCTTAAAATCATCCTAATTTCATGQGCTCT-GCGGCCACG tcctctcattccatcatcctccttaaaatcatcctaatttcatgggccct-gaggccacg tcctctcattccatcatcctccttaaaatcatcctaatttcatgggccct-gaagccagg TCCTCTCATTCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAGGCCACG TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG TCCTCTCATCCCATCATCCTCCTTAAAATCATCCTAATTTCATGGGCCCT-GAAGCCAGG toctctcattccatcatactccttaaaatcatcctaatttcatgggtcct-gaagccagg tcctctcattacatcatcatccttaaaatcatcctaatttcatgagccct-gaagacagg cccactcatttcatcaccatccttaaaatcatcctaatttcatgggccat-gaagccagg tcctctcattcca'tcatcctccttaaaatcatccgaatttcatgagcccttgaagccagg tcctctcattccatcatcctccttaaaatcatccgaatttcatgagcccttgaagccagg tttccagaaagcatgatgtcaagttggag-gtggagcgctgctgggcttgtgaagggtct GGCCCC --- TCCA-CATTCCTGGCCATGTCATCCAGCCCTCACAGGCATT ---------G TAGCTATACATAGTTATT-TTGT TGAAAGATTCTGCTGCTAATATTATTCAATATTTTTG CTTGGCGCC crrGGCocc 404 402 357 357 357 378 380 382 382 385 379 381 384 384 386 389 370 464462 417 417 417 438 GCTGTTTCTTrACACCTCGAGACCrrGGCGCCGGGCCTCAATTCTGCTCCAGTGCTTACT gctgtttctttaaaactagaggccttggcgccgtgcctcaattctgccctgttccttact gctgtttctttaaaactagaggccttggcgccgggcctcaattttgccctgttccttact GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC C3CTGTTTCTTTACACCTAGAGGCCTTGGCGCCX3GGCCTCAATTCCGCCCTGTTCCTTACC GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGC3CCTCAATTCCGCCCTGTTCCTTACC 440 GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC 442 GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCCGCCCTGTTCCTTACC 442 GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTGCTTACT 445 GCTGTTTCTTTACACCTAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT 439 GCTGTTTCTTTACACCCAGAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT 441 GCTGTTTCTTTACACCCAGAGGCCTTGGCGCCX3GGCCTCAATTCTGCCCTGTTCCTTACT 444 GCTGTTTCTTTACACCCAaAGGCCTTGGCGCCGGGCCTCAATTCTGCCCTGTTCCTTACT 444 'CGAGTCCAAGTGAGGGCGGGT TGTGAAGGGTCTCCTCTCAAAGCTGACCGACTTCGGGAC 446 GCTGCTGGCTTCCATAGAGCfCAAGCAGGAAGCTGCCCTTGAATTTCTCATGTTCACTGTG 4 4 3 TATGCTGGC - GCAAATAAGGAAATTTACATCGTCTAATAAAAATTATTTATCAATTTAT 428 GTCTAAGACATTTTGGGAAAATCCCTAGAGC-CTGGATCTTCAATCCTGGTAAGCCAGAG 523 GTCTAAGACATTTTGGGAGAATCCCTAGAGC-CTAGATCTTCAATCCTGGTAAGCCAGiWS 521 GTCTAAGAAAGGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG 476 GTCTAAGAAAGGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG 476 GTCTAAGACATGTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGCTAAGCCAGAC GTCTAAGACATGTTGGGAAAATCCCTAGAGC - (: * CAGGJ GTCTAAGACATGTTGGGAAAATCCCTAGAGC -CAGGATCTTCATTCCTGCTAAGC (

3ATCTTCATTCCTGCTAAGO: TAAG is :ΤΑΑΟΛ

CAGAC CAGAC Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 5,568,521 a a GTCTAAGACATGTTGGGAAAATCCCTAGAGC -caggatcttcattcctgctaagccagac gtctaagacatgttgggaaaatccctagagc-caggatcttcattcctcctaagccagac CTCTAAGACATGTTGGGAAAGTCCCAAGAGC-CAGGATCTTCATTCCTGGTAAGGCAGAC GTCTAAGACATTTTGGC3AAAATCACTAGAGC -CAGGACCTTCATTCCTGGTAAGCCAGAG GTCTAAGACATTTTGGGAAAATCCCTiiGAGC-CAGGATCTTCATTCCTGGTAAGCCAGAG GTCTAAGACATTTTGGGAAAATCCCTAGAGC-CAGGATCTTCATTCCTGGTAAGCCAQAG GTCTAAGACATTTTGGGAAAATCCCTAGAGC - CAGGATCTTCATTCCTGGTAAGCCAGAG ttcccagggattgttgttgagtccacogctcacgtcgtccacactc-tgaggtcccatgg GATGCCATGAAAAAGGCATGCCTTCCCGGGCACAAOCAGGTAGATC-ATCACTCCAAGG AAAACAGTAAAAATTTCATAG- - Aatggggctaagaatctgcactgcaaactaactcttt 476 497 499 501 501 504 498 500 503 503 505 507 486 -90- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention Description (89) 9 10 3 11 2 a a 5 3 2_b 4 l_b 15 20 25 30 6 8 5 2 l_a

AGCCTGGAGACACACCCAAATTATGTCCCTCTTAGTTCAGGGAACATGTCCATTTTCGTC 583 AGCCTGAAGACACACCCAAATTATGTCCCTCTTAGTTCAGGGAACATGTCCATTTTCGTC 581 AGCCTGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGTC 536 AGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGTC 5 3 6 SACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC 536 AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC 557 AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC 559 AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC 561 AGCCGGAAGACACACCCAAATTCTGTCCCTCTTACTTCAGGGAACATGTCCACTTTCGGC AGAATGAAGACACACCAAAATTCTGTCCCTCTTAATTCAGGGAACGTGTCCACTTTCGTC agccgaaagacacacccaaattctgtccctcttagttcagggaacaggtctactttcgtc AGCeTGAAGACAO «XCAAATGCTGTCCCTCTTAGTTCAGGGAACATGTCCACTTTCGTC AGCCTGAAGACACACCCJ

CAAATGCTCTCCCTCTTAGTTCAGGGAACATGTCCACTTTCGTC rAAATGCTGTCCCTCTrAGTTCAGGGAACATGTCCACTTTCGTC CATCTACTCTCCTCTCTCTCTTCCTTACCTCCTTTG-ACACCACCCTCATCCACCAAATATTTGGAGGGTACTCGAGATCTCAAATCAAGTGTCTCC CAGTTGATTTTATGCACAGAAATTATTGAGJUTCCCCTTATCTAGATCCAACAGATCTGG ATTCGCCACCCAC. :ACCC :ATTC,

lATCCCCTC 561 564 558 560 563 563 565 567 546 8.98.10 8.3 8.11 4.2_a 4.5 4.3 4.2_b 4.4 4.1-b 8.1

\CAA1 SCAAT 8 7 8 6 8 S 8 5 35 8 2 4 1-a 8 9 40 8 10 8 3 8 11 4 2_a 4 5 45 4 3 4 2_b 4 4 4 lb 8 1 50 8 7 8 6 8 8 8 5 8 2 55 4 la 643 AGCACTAAAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATGCAATGCGTAACTG 541 aGCATTACAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCATAAATG 596 agcattacaatttttgcaccaaatgtgctaactgcaattccaccatacaatgcataaatg S56 AGCATTACAATTTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG 596 AGCATTACAATCTTGGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCGTAACTG 617 AGCATTACAArTTTGGCACCAAATGTGCrAACTGCAATTCCACCATACAATGCGTAACTG 619 AGCATTACAATTTTCGCACCAAATGTGCTAACTOCAATTCCACCATACAATGCGTAACTG 621 AGCATTACAATTTTGGCACCAAAtGTGCTAACTGCAATTCCACCATACAATGCGTAACTG 621 AGAATTAAAATTTTTGCACCAAATGTGCTAACTGGAATTCCACCATACAATGCATAACTG 624 AGCATTACAATTTTTGCACCAAATGTGCTAACTGCAATTCCACCATACAATGCATAACTG 618 AGCATTAC ^ TTTTTGCACCAAATGTACTAACTGCAATTCCACCATACAATGCATAACTG 620 AGCATTACAATTTTTGCACCAAATGTACTAACTGCAATTCCACCATACAATGCATAACTG 623 AGCATTACAATTTTTGCACCAAATGTACTAACTGCAATTCCACCATA CAATGCATAACTG 623 CCCA-TATGCTACCCCCCGCTACCAAACCTCTGCCAAGCATACCACCCTATCGGCAGCTA 624 ACTGCCACOTCATTTCAGACACTTTTGGCCCTTACCTQGACATCGCXXTCGATATCCAGG 627 GCTTACATAGGTGCTATCAAGACTTAAGGAAOftAAATTTTCCTGACTCTATCCATACCTC 606 GAAATAGAGGCAACATCTCATATCCTGAACAATTCATGTG ------ AGAATCTAGGAGAC 697 TAAATGGAGGCAACATCTCAGATCTTGAACAATCGATGCG ------ AGAA.TCCAGGAGAC 695 GAAATGGAGGGAACATCTCAGATCCTGAACAATCGATGCG ------ AGAATCCAGGAGA? 650 GAAATGGAGGGAACATCTCAGATCCTGAACAATCGATGCG ------ AGAATCCAGGAGAT 650 GAAATGGAGGCAACATCTCCGATCCTGAACGATCGATGCG ------ AGAATCCAGGATAT 650 GAAATGGAGGCAACATCTCCGATCCTGAACOATCGATGCG ------ AGAATCCAGGATAT 671 GAAATGGAGGCAACATCTCCGATCCTGAACGATCGATGCG ------ AGAATCCAGGATAT 673 GAAATGGAGGCAACATCGCCGATCCTGAACGATCGATGCG ------ AGAATCCAGGATAT 675 GAAATGGAC3GCAACATCTCCGATCCXGAACGATCGATGCG ------ AGAATCCAGGATAT 675 GAAATGGAGGGAAAATCCCAGATCATGAACAATCAAAGCG ----- -AGAATCTAGGAGAC 678 GAAATGGAGGGAACATCTCAGATCATGAACAATCGATGAG------AGAATCCAGGAGAT 672 GAAATGGAGGGAACATCTCAGAGCATGAACAACTGATGCG------AGAATCCAGGAGAT &amp;?4 GAAATGGAGGGAACATCTCAGACCATGAACAATCGATGAG ------ AGAATCCAGGAGAC 677 GAAATGGAGGGAACATCTCAGACCATGAACAATCGATQAG ------ AGAATCCAGGAGAC 677 CTCTTCACTCCCA-ACTACATCGGCCGCATCNNNNNNNNN ------ NNNNNNNNNNNNNN 677 AAGCTCAGAGTGTCAAGCAAGCTTTGGAACAGTTGGTGAAGCCCGAAGAACTCAATGGAG 687 CAATTAGTAATAGATCTAGAGATTTAAAACTGAAATCCAOACCTC -CTOCTTCCATGTGC 665 • 91-

This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. Invention description (90) 15 20 25 30 40 45 50 9 10 3U 2—a 5 3 2 a b 4 l_b 1 768 521 a a 9 10 3 11 2_a 5 3 2_b 4 Xb 68 5 2 l_a 9 10 3 11 2 a a 5 3 2-b 4 1-b ACACCGCTTATTTTTGCCTTTTCCCACTGAAACAATGGCTAGTATTAACAATGTrATGCT ACACGGCTTATTTTTGCCTTTTCCCACTGAAACAAGGGCCAGTATTAACAATCTTATGCT ACACGGCTGATTTTTGCGTTTTCCCTGTGAAACAAGGGCCAGTATTAAAAATGGTATGCT 710 ACACX ^ CTGATTTTTGCGTTTTCCCTGTGAAACAAGGGCCAGTATTAAAAATGGTATGCT 7X0 GCACGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT 710 σοΑοσσοϊτΑττττσσοοττττςοοΑοτσΑΑΑΟΑΑοοσαοΑστΑΤΤΑΑΑΑΑτσοοΑΟΟΟΤ 731 GCAC&lt;GCAO CCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT 735 ACACGGCTTATrTPGGCCTTTTCCCACTGAAACAAGGACCAGTATTAAAAATGGTATGCT 738 ACACGGCTTATTTTTGCCTTTTCCCTGTGAAACAAGGGCAAGTATTAAAAACTTTATGCT 732 ACACGGTTTATTTTTGCCATTTCCCAGTGAAACAAAAGCCAGTATTAAAAAGGTTATGCT 7 34 ACACGGCTTATTTTTGCCTrTTCCCTGTGAAACAAGGGCCAGC ATTAAAAAGGTTATGCT 737 ACACGGCTTATTTTTGCCTTTTCCCTGTGAAACAAGGGCCAGCATTAAAAAGGTTATGCT 737 NNNNNNNNNNMNNNNNMNNMNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN

TTTGGCCTTTTCO CGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGCACGCT 733 CGGCTTATTTTGGCCTTTTCCCACTGAAACAAGGGCCAGTATTAAAAATGGTACGCT 735 CGGCTTATTTTGGCCT

AGTGTCCTTTCACTGTCCTGTTTTGC GAAGAGGATTTGAGAATAAATGAC 725 ATCCTTGGTTTCACTCCCCACTTTTAAATCTCTCGGATGTTTACTTCT-TGAGACAGG-- ATCCTGGGTTTCACTCTCTCX ^ TTAAATCTCTCCAATGTTTTCTTCT- TGAGACAGG - ATCCTCTGTTT ^ CTCCCTGCTTTTAAGTCTCC - GATGTTT-CTTCT-TAAGACAGG - ATCCTCTGTTTCACTCCCTGCTTTTAAGTCTCC - GATGTTT-CTTCT-TAAGACAGG - ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC - TGAGACAGG --ATCCTCTCTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC-TGAGACAGG- -ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCC - TGAGACAGG - ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGT-TTCTCCCrTGAGACAGG - ATCCTCTGTTTCACTCCCTGCTTTTAAACGTCTCTGATGT-TTCTCCC-TGAGACAGG - ATACnxrreTTTCACTCCCTGCTTTT'AAACGTCTCCGATGT-TTCTTCT-TGAGACAGG-- gtcttctatttcactgcctgcttttaaacgtctccgatgtattcttot ACCCTCTGTTTCACTCACTGCTTTTAAACGTCTCCGATGTTTTCTTCT ATCTTCTCTrTCACTCCCMCTTTTAAACGTCTCCGATGTTTTCrrCT-TCAGACAGG - ATCTTCTGTTTCACTCCCTGCTTTTAAACGTCTCCGATGTTTTCTTCT-TCAGACAGG - NNNNNNNNNNNNNNNNNCTGCT ^ TTAAACGTCTCCGATGTTTTCTTCT-TCAGACAGG TACACACTTCTGCCAAGATCCTCATCCTCGTATTGAAGAGATTCTCCGATGTCACAGGCA CACATGATTCAACTCCTCCTCAGCTCTGAGCAAT-ATAGCCCTGTCCTGGCAAACAAGAA «Meeting * 814 764 764 764 787 789 791 791 794 789 791 794 807 784 Ministry of Economic Affairs Intellectual Property Bureau Staff Consumer Cooperative Printed 55 6 8 52 1-a GCGTC-ACTGCCGTCACCX!ACGCTTTTCTACGGT----- -GTAATTTTTGTTGTTCGCTT 867 GCCTC -ACTCCCGTCACCAGGGCTTTTCTACGGT------GCAATTTTCGGTGTTTGCTT 865 GCCTC - ACTTCCTTCCCCCTGACTTTTCTACGGT------ATAATrTTCGTTGTTTGCTT 81*7 GCCTC-ACTTCCTTCCCCCTGACTTTTCTACGGT------ATAATTTTCGTTGTTTGCTT 817 GCCTC -ACTTCCGTCAGCCGGGCTTTTCCACGGT------ATAATTTTCCTTGTTTGCTT 619 GCCTC -ACTTCCGTCAGCCGGGCTTTTCCACGCST------ATAATTTTCCTTGTTTGCTT 340 GCCTC - ACTTCCGTCAGCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT 842 GCCTC - ACTTCCGTCAGCCGGGCTTTTCTACGGX------ATAATTTTCCTTGTTTGCTT 844 GCCTC - ACTTCCGTCAGCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT 844 GCCTC-ACTGCCGTCCGCCGGGCTAT-CTAGAGT-- ----ATAATTTTCAGTGTTTGCTP 046 GCCrrC-ACTACTGTCACCTGGGCTTTTCTAAGGT------ATAATTTTCCTTGTTTGCTT 842 GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT------ATAATTTTCCTTGTTTGCTT 844 GCCT C - ACTCCCGTCACCCGGGCTTTTCTACGGT ------ ATAATTTTCCTTGTTTGCTT 847 GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT ------ ATAATTTTCCTTGTTTGCTT 847 GCCTC-ACTCCCGTCACCCGGGCTTTTCTACGGT ------ ATAATTTTCCTTGTTTGCTT 847 ACAAA-ATTGCCAAGAATGTGCAATATCCTGAGTGCCTTGACATGCAGCCATACATGTCT 866 GCTCCTGCAGTAGTAGAGGAGGCAAATATACGTT ---- CACTAATCTAACATACAAG --- 637 -92- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm). 1312008 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed five Ming Mingfa 91 152025303540455055 o la_ b_ba-9131253241176Θ521 88884444448888884

TTGTCAAATTTAGAAATTTTCATrrCA-TCTCTATCAAATGTTGCTCCATT ---- ATCA TTGTCAAATTTAGAACTTTTCATTTCA - TCTCTATCAAATGTTGATCCATT ---- ATCA TTGTCAAAATTAGAACTTTTTATTTCA - TCTCTATCAAATGTTGATCCATT ---- ATCA TTGTCAAAATTAGAACTTTTTATTTCA-TCTCTATGAAATGTTGATCCATT ---- ATCA TTGTCCAAATTAGAACTrrrTATTTCA- -CCTCTAGGAAACGTTCATCCATT ---- ATCA TTGTCCAAATTAGAACTTTTTATTTCA - CCTCTAGGAAACGTTGATCCATT --- - rAAATTAGAACTTTTTATTTCA - TCTCTAGGAAACGTTGATCCATT ---- ATCA ^ CAAATTAGAACTTTTTATTTCA - TCTCTAGGAAACGTTGATCCATT ---- ATCA TTGTCCAAATTAGAACTTTTTATTTTA - TCTCTAGGAAACGTTGATCCATT ---- ATCA 898 TTGTCAACCTTAGAACATTTTATTrCG - TCTCTATGAAATGTTGATCCATT ---- ATCA 900 TTGTCAAAATTAGAACATTTTATTTCA - TATCTATGAAATGTTGATCCATT ---- ATCA 896 TTGTCAAAATTAGAACTTTTTATTTCA - -TCTCTATGAAATGTTGATCCATT ---- ATCA 898 TTGTCAAAATTAGAACTTTTTATTTCA - -TCTCTATGAAATGTTGAGCCATT ---- ATCA 901 TTGTCAAAATTAGAACTTTTTATTTCA - - TCTCTATGAAATGTTGAGCCATT -: - - ATCA 901 TTCTCAAAATTAGAACTTTTTATTTCA-TCTCTATGAAATGTTGATCCATT ---- ATCA 901 ACACAGGACCTCTTGTCTATGTCCTCTATGCTGTGCTGGTCCACGCCGGGTGG 9 2 6 3CAA

TTGTCCA TTGTCCA

CAGCAGAAI —GCAGTA iTAGGCACTGTACCATAAACAAG-ACACTGTGGGGGTTCAGACCAGG- - _ 921 919 871 871 873 894 896 898 891 o 1 - 9131253241176852 88884444448888884 a. b- b- a.

CATACGTATGA- AAATATTATCACGCGTGCTGTGAGATACGTTGTTTTTATTTTCATCAA 9 8 0 CATACGTATGA-AAATATTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAA 978 CATACGTATGG-AAAGACTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAA 930 CATACGTATGG-AAAGACTATCACCCATGATGTGAGATACGTTGTTTTTATTTTCATCAA 930 CATACGTATGG - AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA 932 CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA 953 CATACGTATGG - AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA 955 CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGTTTTTATTTTCATCAA 957 CATACGTATGG-AAATATTATCACACATGCTGTGAGATACGTTGrTTTTATTTTCATCAA 957 CATACACATGG -AAATATTATCACCCACGGTGTCAGATACGTTGTTTTTATTTTCATCAC 959 CATACGTATGG-AAATAGTATCACCAATGCTGTGAGATAAGTTGTTTTTATTTTGGTCAA 955 CGTAAGTATGG-AAATAGTATCAGCCACGCTGTGAGATACGTTGTTTTTATTTTCATCAG 957 CATACGTATGG-AAACAGTATCACCCATGCTGTGAGATACGTTGTTTTTATTTTCATCAG 960 CATACGTATGG - AAACAGTATCACCCATGCTGTGAGATACGTrGTTTTTATTTTCATCAG 960 CGTAAGTATGG-AAATAGTATCAGCCACGCTGTGAGATACGTTGTTTTTATTTTCATCAG 960 AGTTGTCACAACGGA CATTACTTCTCPTATGTCAAAGTTCAAGAAGGCCAGTGGTATAAA 986 ^ TAGGGCT 3TGGGGATTG- rTAGTAAAGTCTAGGAAGTGTTCACTAACAAAATGTCTAA 948 888Θ4444448888884 ο X a 9 ^ 3 ^ 253 2-4 1176852 b- TTCTTTTGTAAAACAAAGGTTATAGTTGGGATACCTTCTGATTTCTCAAGTTTTTTGTTT TTCTTTAATAAACCAAAGGTTATAGTTGGGATACCTTCTGATTTCTCAAGTTTTTrOTTT TTCTlTAATAAACCAAA ^ TTATAGTTGGGATACCTTCCGATTTCrCTAGTTTTTTGTTT TTCrrTAATAAACCAAAGGTTATAGTTGGGATACCrKXXSATTTCTCTAGTTTTTTGTTT TTCTTTAATAAACAAACGGTTATACSCTGGGATACCTTCTGAGTTCTCAAGTTTTTTGTTT TTCTTTAATAAACAAACaSTTATAGCTGGGATACCrrCTGAGTTCTCAAGTTTTTTGTTT TTCTTTAATAAACAAAAGGGTATAGCTGGGArrACCTTCTGAGTTCTCAAGTTTTTTGTTT ttccttaataaacaaaaggttatagctgggataccttctgagttctcaagttttctgttt ttc-ttaataaacaaaaggttatagctgggataccttctgagttctcaagttttttgttt TTC --- AAGAAAAAAAMGGTATAGTTGGGATACCTTCTGATTTCTCAAGATTTTTCTTT TTCTTTAATAAACAAAAGCTTATAGTTGGGATACCTTCTGATTTCTCAAGGTTT, TTATTTAATAAACAAAAGCTTATAGTTGGGATACCTTTGGATTTCTCAAGTTTT * TTCTTTAATAAACAA ^ GCTrATAGTTGGGATACCTTTGGATTTCTCAAGTTTTTTGTTT TTCTTTAATAAACAAAAGCTTATAGTTTGGATACCTTTGGATTTCTCAAQT TTTTTGTTT ttatttaataaacaaaa^ttatagttgggatacctttggatttctcaagtttttttttt ATGGATGATGCCGAGGTC^CTGCCTCTGGCATCACCTCTG---TCCTGAGTCAACAGGCC TTATTAACTAAACTAAATGGTTTC-TCAACATGACCTAATTAATTGTAACTTACTATAAA * * Avian * *

TTTGTTT TTTG1TP 1040 1038 990 990 992 1013 1015 1017 1016 1016 1015 1017 1020 1020 1020 1043 1007 -93·

This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008; Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (92) 10 15 20 25 30 35 40 45 50 55 10 3112_a 5 3 2 a b 4 l_b 1 7 6 8 5 2l_a 10 3112_a 5 3 2_b 4 l_b 6 8 5 2l_a 9 10 3112_a 5 3 2—t&gt; 4l_b 1 7 6 8 5 2l_a CATGTTTTCTT--------- ----------------------------------------1051 CAGGTTTTCTT-------- -----------------------------------------1049 CATGTTTTCTTTCTTTTTTTTTTTTTTTTTTTT------- --GAGACGGGGTCTCGCTCT 1041 CATGTTTTCTTTCTTTTTTTTTTTTTTTTTTTTTTTTrrTGAGACGGTOTCTCGCTCT 1050 CGTGTTTTCTT------------------------------------------- ------1003 CGTGTTTTCTT------------;----------------------------- --------1024 CGTGTTTTCTT---------------------------------------- ----------1026 CGTGTTTTCTT-------------------------------------- -----------1028 CGTGTTTTCTT------------------------------------- ------------1027 CATATTTTCTT------------------------------------ -------------1027 CATGTTTTCTT---------------- ---------------------------------1026 CAAGTTTTCTT—.------------- ----------------------------------102B CATGTTTTCTT-------------- -----------------------------------1031 CATGTTTTCTT------------- ------------------------------------ 1031 CAAGTTTTCTT------------ ------------------------------------- 1031 TATGTCCTCTTTTACATCC----------- ------------------------------1062 TGGTTGTTTGTTCA------------------ ----- 1021 ★ * # GTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGCTCCGCT 1101 GTCGCCCAGGCCGGACTGCGGACTGCAGTGGCGCAATCTCGGCTCACTGCAAGCTCCGCT 1110 TCCCGGGTTCACGCCATTCTCCT (X: CTCAGCC * ICCCGAGTiy3CrGGC3ACTACftGGCGCGC 1161 TCCCGGGTTCACGCCATTCTCCTOICTCAGCCTCCCGAGTAGCTGGGACTACAGGCGCCC 1170 -94- this paper scales applicable Chinese national standard (CNS) A4 size (210 X 297 mm) A7 B7 1312008 V. DESCRIPTION OF THE INVENTION (93) 8 9 Θ 10 S 3 8 U 5 4 2_a 4 5 4 3 4 2_b 4 4 10 4 l_b 8 1 3 7 8 6 8 8 15 8 5 8 2 4 1 - a 20 8 9 8 10 8 3 8 11 4 2"a 25 4 5 4 3 4 2_b 4 4 4 30 8 1 8 7 8 6 8 8 8 5 35 8 2 4 1 one a 8 9 40 8 10 8 3 8 11 4 2 a a 4 5 45 4 3 4 2_b 4 4 4 l_b 8 1 50 8 7 8 6 8 3 8 5 8 2 55 4 l_a Printed by the Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperatives---A------------------------------ -------------------------- 1052 A---------------------- --------------------......-.......1050 GCCACCGCGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACTTTGTTAGC 1221 GC - ACCGCGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCTTGTTAGC 1229 CAGGATGGTCTCGATCTCGBGACCTCATGATCCACCCGCCTCGGCCTCCCAAAGTGCTGG 1281 CAGGATGGTCrCGATCTCCTGACCTCATGATCCACCCGCCTCGGCCTCCCAAAGTGCTGG 1289 ----- -----------------------------------------AACTGCCGTCGCAC 1066 ------- ---------------------------------------AACTGCCGTCGCAC 1064 GATTACAGGCGTGAGCCACCGCGCCCGGCCGTTTCATGTTTTCTTAAACTGCCATCGC AC 1341 GATTACAGGCGTGAGCCACCGCGCCCGGCCGTTTCATGTTTTCTTAAACTGCCATCGCAC 1349 ---- -----------------------------------------AAACTGCCGTCGCAC 1018 ------- --------------------------------------AAACTGCCGTC GCAC 1039 ---------------------------------------------AAACTGCCGTCGCAC 1041 - --------------------------------------------AAACTGCCGTCGCAC 1043 ---- ------------------------------------------AAACTGCCGTCGCAC 1042 ------ ---------------------------------------AAACTGCCGTCGGAC 1042 --------- ------------------------------------AAACTGCCGCCGCAC 1041 ------------ ------------------------------...AAACTGCCGCCGCAC 1043 ----------------- ----------------------------AAACTGCCGCCGCAC 1046 -------------------- -------------------------AAACTGCCGCCGCAC 1046 --------- AAACTGCCGCCGCAC 1046 ------------ -------------------------ACAAGAGTGAATGGGAAAGACAC 1085 ----------------------- ---------------------TAAACCTTAATCTTTT 1037 -95- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (94 8 9 8 10 8 3 8 11 5 4 2 a 4 5 4 3 4 2_b 4 4 10 4 l_b 8 1 8 7 8 6 8 8 i5 8 5 8 2 4 l_a 20 8 9 8 10 8 3 8 11 4 2_a 25 4 5 4 3 4 2_b 4 4 4 l_b 30 8 Q 1 τ 0 8 r 6 3 8 3 5 35 3 2 4 l_a 8 9 40 8 10 8. 3 8 11 4 2_a 4 5 45 4 3 4 2_b 4 4 4 l_b 8 1 50 a 7 8 6 8 8 8 5 8 2 55 4 GTCCAAAACCACTCGCTATGCAATGTCTTG-ACCATCTCTCTTTTCTGGCAAATATAAAT GTCCGAAACCATTCACTATACAATGTCATT-TTCATCTCTCTTTTCTGGCACACATAAAT atccgaaatcattcactatacaatgxcatg-accatctctctttcctggcaaacataaat ATCCGAAATCATTCACTATACAATGTCATG-ACCATCTCTCTTTTCTGGCAAACATAAAT GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT GTCCGAAACCGCTCACTATGCAGTGTCATG - ACCGTCTCTCTTTTCTGGCAAACATAAAT GTCCGAAACCGCTCACTATGCAGTGTCATG-ACCGTCTCTCTTTTCTGGCAAACATAAAT GTCCGAAACCGCTCACTATGCAGTGTCATG- ACCGTCTCTCTTTTCTGGCAAACATAAAT GTCAGAAACTACTCACTATACAATGTCGTG-ACAATCTACATTTTCGGGCAAACACAAAT GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGCACACATAAAT GTCCGAAACCACTCACTATACAATGTCAGG-ACCATCTCTCTTTTCTGGCACACATAAAT GTCCGAAACCACTCACTATACAATGTCAGG-ACCATC TCTCTTTTCTGGTACACATAAAT gtccgaaaccactcactatacaatgtcagg-accatctctcttttctggtacacataaat gtccgaaaccactcactatacaatgtcjw ^ -accatctctcttttctcgcacacataaat agtgagagtgtgtcaagaggcaggga-acc-aagagccctcggcgctga GCCAAAATATTTGTAGCTTATGTTCCCATTTAACAAGGTTTTCTGGTC. SA-AGACACAGAC CAAAACTGTGCAC * «1125 1123 1400 1408 1077 1098 1100 1102 1101 1101 1100 1102 1105 1105 1105 X142 1097

^ GGTGATTGCATTATTTCCCCAAAGTCTTTTACA TTGCGGAATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTACACA TTGCSGGATTGTCATCAATTAGTCTCTCAGTGACTGCATGATTTCCACAAAGTCTTTCACA TTGGC ^ ATTGTCATCAATTAGTCTCTCGGTGACTGAATGAriTCCACAAAGTCTrrCACA TT ^ GGGATTGTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA TTGGGGATTOTCATCAAOTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA TTGGGGATTGTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGGCTTTCACA TTGGGGATTGTCATCAATCAGTCTCTCOSGGATTGCATGATTTCCCCAAAGGCTTTCACA TTGGGGATTGTCATCAATTAGTCTCTCGGGGATTGCATGATTTCCCCAAAGQCTTTCACT: AATTAGTCTCI CAAATAGTCTCC CAATTAGTCTCl

TTGGGGAATGTCATCAATTAGTCTCTCCSGTGATTGCATGATTTCCCCAAAGTCTTTCACA TTGGGGAATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA GAAAGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA GAAAGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA TTGG (^ AATGTCATCAATTAGTCTCTCGGTGATTGCATGATTTCCCCAAAGTCTTTCACA A-CGCGAGCAACG-CAAGGAGAGCTCAAGAGAGACTACCCCTGCCTCCAGG-TACCCGA CCACATCATTCTAATGAACTTAGTGTCCAATAAAACATGGACTCTCAGTC (3TCCCACGGA ★ * * * * GTCTAGTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACGACTTAATGC CTCTACATTGTGCACTGAGTATCTCTTCAGACTTTAGTGCATGTTTCTACCACTHGATGC GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGT - GTTTCTACCATATGATGC gtctactttatccactgj ^ atctctptcaaacttcagtgtatgtttctaccatttgatgc G &gt; TCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTCATTC GTCTACTTTGTGCACTGAGTATCTCrrrCAAACTTCAGTGCATGTTTCTACCATTTCATCC GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTrrCTACCATTTGATTC GTCTACTTTGTGCACTGAGTATCTCnrrCAAACrrCAGTGCATGTTTCTACCATTTGATGC GTCTACTTTGTGCACTGAGTATCTCTTCAAACTTCAGTGCATGTTTCTACCATTTGATGC gtctacattgtgc Actgagtatctcttcaaacttcagtgcttctttctaccatatgatgc GTCTACTTTGTGCAATGJUSTATCTCTraVAACTTCAGTGCATATTTCTACCATTTGATGC GCCTACTTTGTGCACTGAGTATCTCTTCAAiCTrTAGTGCATGTTTCTACCATTTGATGC

TTGGGGA TTGGGGA 1185 1183 1460 1468 1137 1158 1160 1162 1161 1160 1160 1162 1165 1165 1165 119» 1157

: TTCAGTGCATGTTTCTA rrTTAQTGCATOTTTCTii GCCTACTTTGTGCACTGAGTATCTCTrCAAACTTTA GTTGGACGAGCACTTGGTGGAAAGAGCCACTCAGGAAAGCAC -CTTAGACCACTGGT ^ AI agttattttgtgtgcatagtacatctctgtgaatatgcctaatgaggtatggaaggacac * * will * 1245 1243 1518 1528 1X &amp; 7 1218 1220 1222 1221 1220 1220 1222 1225 1225 1225 1257 1217 • 96 · This paper scale applicable to Chinese National Standard (CNS) A4 size (210 X 297 mm) 1312008 V. Invention description A7 B7 10 15 20 25 30 35 β 9 8 10 8 3 8 11 4 A 5 4 3 4 2—b 4 4 4 1—b 9 1 9 7 3 6 d 8 3 5 8 2 4 1-a β 9 Β 10 β 3 β 11 4 4 5 4 3 4 2-t&gt; 4 4 4 8 1 8 7 β 6 8 β 8 5 8 2 4 1 a

-TTTTC -TGTTC rrTATTATTCAGCAATCTAGCTrCCACAAGAGCATTTAATGTAAAGACTTGTCT ··: TTTATTACTT -gccatctagcttccacaagagcatttcatgcaaagactt TTTATTATTTGGCAACCTAGCTTCCAAAAGAGCATTTCATGCAAAGACrTGTCT-TGTTA TTTATTATTTGGCAACCTAGCTTCCAAAAGAGCATTTCATGCAAAGACTTGTCT-TGTTA TTTCTTATTTGGCAATCTAGCTTCCACAAGAGCATTTCACGCAAACSACTTGTCr-TGrPC tttcttatttggcaatctagcttccacaagagcatttcatgcaaagacttgtct-tgttc tttcttatttggcaatctagcttgcacaagagcatttcatgcaaagacttgtct-tgttc TTTATTATTTGGCAATCTAGCTTCCACAAGftGCATTrCATGCAAAGACTTGTCT-TGTTC TTTATTATTTGGCAATCTAGCTTCCACAAGAGCATTTCATGCAAAGACTTGTCT-TCTrC TTTATCATTTGGCAATCTAGCTTCCACAAGAGCATrrCATGCAAACACTTGTCT-TGTTG tttattatttggc aacctagcttccacaagagcatgtcaggcaaagagttctct -TCTTC TTTATTATTTX ^ AGCCTAGCTTCCACAAGAGCArTTCATGCAAAGACTTGTCT-TGTTC TTTATT --- TGGCAGCCTAGCTTCCACAAGAGTATTTCATGCAAAGACTTGTCT- TGTTC TTTATT---TGGCAGCCTAGCTTCCACAAGAGTATTTCATGCAAAGACTTGTCT-TGTTC TTTATTATTTGGCAGCCTAGCTTCCACAAGAGCATTTCATCCAAAGACTTGTCT-TGTTC TCCTCCAAGAGCAAAACAAAACGAAGCCTGAGTTCAACGTCAGAAAACTTGAAGGTACCC TT-ATTATCCAAACA GAGACATTCCACTGGTGCTAGAGAGCCACAGAC - GGAAGTTTTC • ♦ · * Cong will «1304 1301 1S77 1587 1256 1277 1279 1281 1280 1279 1279 1281 1281 1281 1284 1317 1274 riUvrTTCACTCOGATAT ^ GAATCArrjUGGCTOAACATGGAAAGGTTAT 1364 TCCACTGGCAGGTAATTTCACTCGGATAGAGAATCAATAGGCTGAACGTGGAAAGGTTAT 1361 TCCACTGGGAGCTAATTTCATTCGGATAGAOAATCAATAGGCrGAACGTQGAAAGCTTAT tccactggcagctaatttcattcggatagagaatcaataggctgaacgtggaaagcttat tccactggcaggtaatttcactcggacagagaatcaataggctcaacgtggaaagcttat caggtaatttcactcagatagagaatcaataggctcaacgtggaaagcttat caogtjuitttcactcggacagagaatcaataggctcaacgtggaaaggttat TCCACTGGCAGGTAATTTCACTCGGACAGftGAATCAATAQGCTCAACGTGGAAAQGTTiiT TCCACTGGCAGGTAATTTCACTCGGACAGAGAATCAATAGGCTCAACGTGGAAAOGTTAT tccactggcaagtaattcaacacggatagagaatcaataggctcaacgtggaaaggttat TCCACTGGAAGGTAATTTCATTCGCACAGAGAATCAATAGGCTGAACGTAGAAAGGTTAT tccactggccagtaatttcactcggatagaoagtcaataggctgaacgtggaw ^ ggttat

CCACTC5GCA CCCCTGGCA TCCACTGGCAGGT. • AATTTCACTCI 'AATTTCACTCi

1637 1647 1316 1337 1339 1341 1340 1339 1339 134X ITCGGATAGAGAATCAATAGTCTGAACGTGGAAAGGTTAT 1341

:TGGCAG :TGGCCA TCCACTGGCCAGTAATTTCACTCGGATAQAGAGTCAATAGGCTGAACGTGGAAAeGTTM? 1344 TGCCTCCCAACGTACrTGTGATTCATCAATCAAAATACAAGTGTGGGATGAAAAA-CCAT 1376 TCTGCCTACTGGAAATAAAGC-----CAAGCTTTCTTCTrTCCTCAGCCGTGAQGATTGC 1329 * * * * * 40 45 o 1_4b_ 913125324 88884444

3TTTGAT 3TTTGAT 50 Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 55 -17 6 8 5 2. -888888 CGCTGGAAa3TCTGTTTGATTCCAC &lt;. ^ TCTCTCCTTTCTTATTAAGGAAAAAGATACAC cgctggaaggtttgtttgtttccacggatctctcctttcttattagggaaaaaaatacqc CGCTGGAAGGTTKSTrrcrTTCCACGGATCTCTCCTTTCTTATTAGGGAAAAAAATACGC CGCTGGAA &lt; »TCTGrrrGATTCCACGGATCTCTCCTTTCTCATTAGGGAAGAAAATACGC cgctggaaggtctgtttgattccacggatctctcctttctcattagggaagaaaatacgo cgctggaaggtctgtttgattccacggatctctcctttctcactagggaaoaaaatacgc CGCTGGAAGGTCTGTTTGATTCCACGGAtCrrCTCCTTTCTCACTAGGGAAGAAAATACGC CGCTGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTCTCACTAGGGAAfiAAAATACGC CGC TGGAAGGTCTGTTTGATTCCACGGATCTCTCCTTTC tcattagggaagaaaatacgc CGCTGGAAGCTCTGTTTCATTCOVCGGATCrerCCTTTCTTATTAAAGAAAAAAATACGC CCCTGGAACSGTCTGTTTGATTCCACGGATCTCTCCTTTATTATTAAGGAAGAAAATACGC CGCTGGAAGGTCTGTTTGATTCCACGGATCTC-rCCrrTATTATTAAGGAAAAATATACGC CGCTGGAAGGTCTGTrTGATTCCACtSGATCTCTCCTTTATTATTAAGGAAAAATATACGC CCCTGGAAGGTCTGTrTOATTCCACGGATCTCTCCTTTATTATTAAGGAAGAAAATACGC ACCCGAt CATCCTGAA - TGACCTCC— -CAGCA i TCTTTA· lAAAGCTCCCTGCTAAAGCl ►TCATTCTCTCTCTCTCT1

SACAGATCAGG 1424 1421 1697 1707 1376 1397 1395 1401 1400 1399 1399 1401 1401 X40X 1404 1434 1387 -97- National Standard (CNS) A4 size (210 x 297 mm)

1312008 A7 B7 V. INSTRUCTIONS (96) 8 9 3 10 8 3 8 11 5 4 2_a 4 5 4 3 4 2_b 4 4 !0 4 l_b 8 1 B 7 B 6 8 Q 15 B 5 8 2 4 1-a 20 β 9 e 10 8 3 8 11 4 2^a 25 4 5 4 3 4 2_b 4 4 4 1 a b 30 δ 1 8 7 S 6 8 8 8 5 35 8 2 4 1 a a 8 9 40 8 10 8 3 8 11 4 2_a 4 5 45 4 3 4 2 - b 4 4 4 1-b 8 1 50 a O 7 c 0 a 0 B 8 5 8 2 55 4 TGTGCTAATTACTGTACTTCATTGCCTATTCTCAGGTCAGAAAG-----CGCACTTCAGA tgcgctaattactatacttcattgactattctcaggtcagaaag----- CGCACTTCCGA TGTGCTAAATACTATACTTCATTGACrATTCTCAGGTCAGAAAG ----- CGCACTTCCGA TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG ----- CGCACTTCCGA TGTGCTAAATACTATACTTCATTGACrATTCTCAQGTCAGAAAG ----- CGCACTTTCGT TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG ----- CGCACTTTCGA TGTGCTAAATACTATACTTCATTGACtATTCTCAQGTCAGAAAG ----- CGCACTTTCGA TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG ----- CGCACTTTCGA TGTGCTAAATACTATACTTCATrGACTATTCTCAGGTCAGAAAG ----- CGCACTTTCGA Tgtgctaaatattatacttcattgactattctcaggtcagaaag----- CACACTTCCGA TGTGCTAAATACCATACrrCATTGACTAATCTCAGGTCAGAAAG ----- CACACTTCCGA TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG ----- CGCACTTCAGA TGTGCTAAATACTATACrrCATTGACTATTCTCACGTCAGAAAG ----- TGCACTTCAGA TGTGCTAAATACTATACTTCATTGACTATTCTCAGGTCAGAAAG ----- TGCACTTCAGA TGTGCTAAATACTATACTTCATTOACTATrCTCAGGTCAGAAAG ----- CGCACTTCAGA AGT-CCATGAACACTGGCACACTCGCTTCTCT --- GCAAGGGAG ----- GAC- CAGGAGA AATAATAAGATAAACTTTGTGCAAGACTTGGTAAGAGTAGAGTGTCTGACACCTTATGGT * * * * * *

r --- CCTTCTATCGCTGAAAGGAT-GATGGTATCTGCCAAAAGCACATAC-TCGGA rTGTCCTTCCATCGCTGAGAGGAT-GATCGTATCTGCCAAAAGCACATAT-TTGGA CTTCTTCTCTTTCCGTCGCTGAGAGGAT-GATGGTAGCTGCCAAAAGCACATAC-TTGGA cttcttctctttccgtcgctgagaggat-gatggtagctgccaaaagcacatac-ttcga cttcttgtccttccgtcgcggagaggat-gatggcagctgccaaaagtacatac-ttgga CTTCrrGTCTTTCCGTCGCTGAGAGGAT-GATGGCAGTTGCCAAAAGTACATAC-TTGGA CTTCTTGTCCTTCCGTCGCTGAGAGGAT -GATGGCAGCTGCCAAAAGTACCTAC - TTGGA CTTCTTGTCCTTCCGTCGCTGAGAGGAT-GATGGCAGCTGCCAAAAGTACCTAC-TTGGA CTTCTTGTCCTTCCGTCGCTGAGAGGAT -GATGGCAGCTGCCAAAAGTAACTAC -TTGGA ATTCrTGTCCTTCGGTCACTGAGAGGAT -GATGGTAGCTGCCAAAAGTACATAC- 'TTTCTTGTCCTTCTGTCGCTGAGAGGAT -GATGATAGCTGCCAAAAGTACATAC-' CTTCTTGTCCTTCCGTTGATGAGAGGAT -GACGGTAGCTGCCAAAAGTACATAC -1 cttcttgtgcttccatcgctgagaggat-gatggtagctgccaaaagtacatac-ttgga CTTCTTGTGCTTCCATCGCTGAGAGGAT-GATCGTAGCTGCCAAAAGTACATAC · CTTCTTGTCCTTCCGTTGATGAGAGGAT-OACGGTAGCTGCCAAAAGTACATAC -------------- GCCAAAGOSAAGA - ACAAACACTGCAAGAGGGCTCTGC * ^ AGCAAAA1:

GCTATAATACTCAAAGCAAAAG 1479 1476 1752 1762 1431 1452 1454 1456 1455 1454 1454 1456 1456 1456 1459 1484 1447

-TTGGA -TTGGA -TTGGA -TTGGA TTGGA :-TTGGA :-TTGTG

I^TCGCCTAGGACCAGAAAAGGGAGTCACATAGGA 1534 1534 1810 1820 1489 15X0 1512 1514 1513 1512 1512 1514 1514 1514 1517 1526 X507 AGT---ACATCCCAGCACAAACACACACACACA---------------CAC6CACACA AGT---ACATCCCGGCACAAACACACACACACA---- -----------CACACACACA GGT---TCATCCCAGCACAAACACACACACACAAA---------------CACACAAACA GGT---TCATCCCAGCACAAACACACACACACAAA------------ --- CACACAiSLACA AGT - TCATCCCAOCACAAACACACACACACACGC - CCCCCCCACACACACACACACA AGT --- TCATCCCAGCACAAACACACACACACACGCGCCX: CCCCCACACACACACACACG GGT --- TCATCCCAGCACAAACACACACACACACACGCCCCCCCC --- CACACACACACA GGT --- TCATCCCAGCACAAACACACACACACACACGCCCCCCCC ----- CACACACACA GGT --- TCATCCCAGCACAAACACACACACACACATGCCCCCCCC ---- ACACACACACA AGT- -TCATCCCAGCACAAACACACATACACACACGCCCCCCCC ----- CACACACACA AGT --- TCATCCCAGCACGAGCACACACACACATAAACACACACA ----- CACACACACA AGT --- TCATCCC AGCACAAGCACACACACACACA- -CACAAACA ----- CACACACACA AGT - TCATCCCAGCACAAGCACACACACACAjCACACAAACA ----- CACACACACA AGT - TCATCCCAGCACAAGCACACACACACACA-CACAAACA -----CACACACACA AGT- - -TCATCCCAGCACAAGCACACACACACACA—CACAAACA-----CACACACACA TGCCAGTGATCTCAGTGGAAGTGCCGACCCACACGTAGGGGAGAA------AAACACACA AATCTAGAAGACCTATTGGCTGAGAGACCTGCAGCCTCATAGTTCATTAGCTCTC-CATA * · * * * * * 1576 1576 1852 X8€2 1544 1567 1566 1566 1566 1564 1564 1564 1564 1564 1567 1530 1566 Ministry of Economics Property Bureau Staff Consumer Cooperative Printed-98- This paper scale applies China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 5, invention description (97) 8 9 8 10 8 3 8 11 5 4 2—a 4 5 4 3 4 2 a b 4 4 10 4 1-b 8 1 8 7 8 6 8 3 15 8 5 8 2 4 l_a 20 8 9 8 10 8 3 8 11 4 2.a 25 4 5 4 3 4 2_b 4 4 4 l_b 30 8 1 8 7 8 6 8 3 8 5 35 S 2 4 Ua 8 9 40 8 10 8 3 8 11 4 2.a 4 5 45 4 3 4 2_b 4 4 · 4 1-b 8 1 50 8 7 8 6 8 8 8 5 8 2 55 4 a AACACACATACTCACAC------------GGTTTCA- - TAGGTAAAGATTTCTTCCCTGA C AC AC AC ACAC AC ACAC- -----------GGTTTCA- - TAGGTAAAGATTTCTTCCCTGA CAC ACACA------CAC------------GGCTTCA- -TAGGTAAAGATTTCTTCCCTGA CACACACA------CAC------------GGCTTCA - - TAGGTAAAGATTTCTTCCCTGA AACACACTCACACACACACACGCACAC - -GGTTTCC - -TAGGTAAAGATrTCTTCCCrGC AACACAATCACACACACACACTCACAC --GGTTTCC --TACGTAAAGATTTCTTCCCTGC AACACACTCACACACACACACGCACAC - GGTTTCC- -TAGGTAAAGATTTCTTCCCTGC AACACACTCACACACACACACGCACAC - -GGTTTCC - -TAGGTAAAGATTTCTTCCCTGC AACACACTCACACACACACACGCACAC - -GGTTTCC - -TAGGTAAAGATTTCTTCCCTGC AACACACTCACACACACACACACACACACGGTTTCC - AAGGTAAAGATTTCTTCCCTGC CACACACACACACACACAGACACACACAGGGTTTOA-TAGGTAAAGATTTCTTCCCTGA CACACACACACAGAGAGAGATACACACACGGTTTCATAGGTAAAGATTTCTTCCCTGA CACACACACACACACACAGACACACACACGGTTTCATAGGTAAAGATTTCTTCCCTGA CACACACACACACACACAGACACACACACGGTTTCATAGGTAAAGATTTCTTCCCTGA CACACACACACAGAGAGAGATACACACACGGTTTCATAGGTAAAGATTTCTTCCCTGA CACACACACACACACAC --- ---------GGTTTCATAGGTAAAGATTTCTTCCCTGA GCAACTCTCACATGAAATGAAGTCAGTGGTGTTTCAAGTGCTTGAAACCCTCTTTACT-C * * * * ** · « * * ♦ Window ·· 1622 1622 1892 1902 1600 1623 1622 1622 1622 1622 1622 1622 1622 1622 1625 1626 1625 CATTCTTTTACCTAAAATAAG- CATTGTTTTACCTAAAATAAG- CATTGTTTTACCTAAAATAAG- CATTGTTTTACCTAAAATAAG- CATTGCTTTACCTAAAATAAG- CATTGCTTTACCTAAAATAAG-

-GCAACTGTGTGGCCACTGTCCCAACCCGGTTACCAT -GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT -GCAACTGTGTGGCCACTGTCCCAACCTGGTTACACT -GCAACTGTGTGGCCACTGTCCCAACCTQGTTACAjCT -GCAACTGTGAGGCCACTGTCCCAACCCGGTTACACT -GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT

MTTGTCC WTTGTCC CATTGCTTTACCTAAAATAAG --- GCAACTGTGAGTCCACTGTCCCAACCCGGTTACACT CATTGCTTTACCTAAAATAAG --- GCAACTGTGAGGCCACTGTCCCAACCCGGTTACACT CATTGCTTTACCTAAAATAAG - GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT CATTCTTTTACCTAAAATAAG - GCAACTGTGCGGCO \ CTGCCCAAACCCGGTTACACT CATTCTTTTACCTAAAATAAG --- GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT CATTCTTTTACCTAAAATAAG --- GCAACTCTGTCGCCACTGTCCCAACCCGGTTACACT CATTCTTTTACCTAAAATAAG --- GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT CATT-CTGTACCTAAAATAAG --- GCAACTGTGTG-CCACTGTCCCAACCCG-TTACACT CATTCTTTTACCTAAAATAAG- --GCAACTGTGTGGCCACTGTCCCAACCCGGTTACACT TACTTCTAAATGTGAATTAAr · ♦ * * # «· # avian * 1679 1679 1949 1959 1657 1680 1679 1679 1679 1679 X679 1679 1679 1679 1679 1633 X683 CATATTATATGTGCCTATCATCCTGAGGAGTAATTT ---- GATGAAGGTGTTTTAGAAGT 1735 CATATTACATGTGTCTATCAQCCTGl ^ QGAGTAGTTT ---- GATTCAGGTGTTCTAGAAGT 1735 CATATTACATCTGCCTATCATCCTGAGGAG.TAATGT ----GATTCAGGTGTTCTAGAAGT 2005 CATATTACATCTGCCTATCATCCTGAGGAGTAATGT----GATTCAGGTGTTCTAGAAGT 20X5 CCTATTATATGTGCCTATCATCCTGAGGAGTAATT T ---- GATTCAGGTGTTCTGGAAGT X713 CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTGGAAGT 1736 CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTGGAAGT 1735 CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTGGAAGT 1735 CCTATTATATGTGCCTATCATCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTGGAAGT 1735 CCTATTATATGTGCTTATCATCCTGAGGAGTAATCT ---- GATTCAGGTGTTCTGGAAGT 1735 aXTATTATATGTGCCTATCACCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTAGAAGT 1735 CATATTATATGTGCCTATCACCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTAGAAGT 1735 CATATTACATGTCnCTATCAGCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTAGAAGT 1735 CATATTACATGTGTCTATCAGCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTAGAAGT 1735 CATATTATATGTGCCTATCACCCTGAGGAGTAATTT ---- GATTCAGGTGTTCTAGAAGT 1735 C ^ TATTACATGTGTCTATCAGCCTGAGGAGTAGTTT ---- GATTCAGGTGTTCTAGAAGT 1733 AAAATAATO \ GGCATTATTCTACTAAGTATTGGTCTCaiTAACTCCTCTATTTTCTTTA0 X743 -99- this paper scales applicable Chinese national standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. Invention description (98) Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printing 15 20 2 5 30 35 40 45 50 55 53 24X176852 1-88884444448888884

Ola- b- b_ -13 12 &quot; o 1 Λ- b- 9131253241176852-88884444448888884 b- a- -1 CATGATGTGGACTGTGTCtGTT: GAATTCCCAGCGATGCAAGGGOACACACCCTOTGACTC CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC catgatgtgggctgtgtctgttgaattcccagcgatgcaaggggacacaccctgtgactc catgatgtgggctgtgtctgttgaattcccagcgatgcaaggggacacaccctgtgactc catgctgtgggctgtgtctgttgaattcccagcgatgccaggggacacaccctgtgactc catgctgtgggctgtgtctgttgaattcccagcgatgcaaggggacacaccctgtgactc CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCCACaSGGACACACCCTGTGACTC CATGCTGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCCAGGGGACACACCCTGTGACTC catgctgtgggctgtgtctgttgaattcccagcgatgccagggcsacacaccctgtgactc CATGATCTKSGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACTC CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAiGGGGACACACCCTGTGACrC CATGATGTGGGCTG ^ GTCTGTTGAATTCCCAGCGATGCAAiGGGGACACACCCTGTGACTC catgatgtgggctgtgtctgttgaattcccagcgatgcaaggggacacaccctgtgactc CATGATGTGGGCTGTGTCTGTTGAATTCCCAGCGATGCAAGGGGACACACCCTGTGACT C catgatgtcggctgtgtctgttgaattcccagcgatgcaaggggacacaccctgtgactc 3TCAGCC * * * * gaaaagttagtctaagacatttggcataaaggctatgcc • * * · * * * * * * * * * # * * * 1795 1795 2065 2075 1773 1796 1795 1795 1795 1795 1795 1795 1795 1795 X795 1799 1803

ArrCCTTAATTGftGTGCTGATATTTGATTGGTinrATTGCCCACCTTATGTGCGGGTGGGG 1855 ATTCCTTAATTCAGTGCTGATATTTGATTGGTTTATCGCGCACCTGATGGGTGGGTGGGG 1855 ATTCCTTAATTAAATGCTGACATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGAGG 2125 ATTCCrTAATTAAATGCTCATATTTGATTGGTTTATCGCGCACCTGATGAGTGGGTGAGG 2135 CTTCCTGAATTCAGTGCrGATATrrGATTGGCTTATCGCCX: ACCTGATGAGTAGGTGGGG 1833 CTTCCTGAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGG 1856 CTTCCTGAATTGAGTGCTGTTATTTGATTCGCTTATCGCGCACCTGATGAGTGGGTGGGG 1855 CTTCCTGAATTGAGT ^ rTGATATTTGATTGGCTTATCGCGCACCTGATGAGTGGGTGGGG 1855 CTTCCTCAATTGAGTGCTGATATTTGATTGGCTTATCGCGCACrTGATGAGTGGGTGGGG 1855 CTTCCTGAATTGAGTGCTGATATTTGATTGGCrAATCGCGCACCTGATGAGTGGGTGGGG 1855 ATTCCTTAATTGAGTGCTGATATTTGATTGTrTTATCGCGCACXrrGATCSGGTGGGTGGGG 1855 ATTCCTTAATTGAGTGCI ^ TATTTGATTGGTTTATCGCACACCTGATGGGTGGGTCGGG 1855 ATTCCTTAATTGAGTGCTGATATTTGArrGGTTTATCGCACACCTGATGGGTGGGTGGGG 1855 ATTCCTTAAT ^ AGTGCTGATATTTGATTGGTTTATCGCACACCTGATGGGTGGGTGGGG 1855 ATTCCTTAATTGAGTGCTGATATTTGATTGGTTTATCGCACACCTGATGGGTGGGTGGGG 1855 ATTCC TTAATTGAGTGCTGATATTTGATTGGTTTATCGCGCACCTGATGGGTGGGTGGGG 1859 AGGAAGGATTCGTGGGGGTCTCCTTGAAAATACTGCAATA-ATCTAAGAAATCTTCAACC 1862 6 8 8 8 4 1 a_ b- _ 12 5 3 2 4- 117 6 8 5 2 1 8 8 8 8 8 8.

TGTTCGCTCTTGGTGCGGGTGAGTTATGTAAGGGCTGATTTGGCCAGAGAACTCGTTATT tgttcgcggttcgtgggggtgagttatataagggctgatgccx ^ agagagctcgtcatt TGTTCGCCGTT &lt; 3GTGGGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT TGTTCGCCGTTCGTGCGGGTGAGTTATATAAGGGCTGATGCGGCCAGAGAGCTCGTCATT tgttcgcggttggtgggggtgacttacagaagggctgatgtggccagagagctcgtcatt tgttcgcggttsgtggggttgacttacagaagggctgatgcg-ccagagagctcgtcatt TGTTCGCGGTTGGTGGGGGTGACTTATAGAAGGGCTGATGCOGCCAGAGAGCTCGTCATT TGTTCGCTGTTGGTGGGGGTGACTTACAGAAGGGCTGATGCGGCCAGAGAeCTCGTCATT TGTTCGCGGTTCGTGGGGGTGACTTACAGAAGGGCTGATGCGGCCAGAGAGCTC CGGCC

TGTTCGCGGTTOGTGTGGGTGAGTTATAGAAGGGCTGATGCG

• CGTCAT CAGACAGCTCGTCAT .GCTCGT lGCTCGI iGCTGGT

CCSGCCAGAGAOCTCGTCATT tgttcgcggttqstgggggtgagttatataagggctgatgcggccagagagctcgtcatt TGTTCGCGGTTGGAGGC5GGTGAGTTATATAAGG (3CTGA.TGCGGCCAGAGAGCTGGTCATT TGTTCGCGGTTGGAGGGGGTGACTTATATAAGGCSCTGATGCGGCCAGAGAGCTGGTCATT TGTTCGCGGTTGGTGGGGGTGAGTTATATAAGGGCTGArrGCGGCCAGAGAGCTCGTCATT ^ TAAGGOCTGXTGCGGCCAGAGAGCCCGTCATT tgttcgcggttggtgggggtgagttatataagggctgxtgcggcc tattgcccctcagtactgttg «gtcccctgtacttgacttttccc 'Yu will eat" * will * * # * * << *

CTTAAGT -ATT **# 1915 1915 2185 2195 1893 19X5 1915 1915 1915 1915 1915 1915 1915 1915 1915 1919 1919 -100- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 Wu Ming DESCRIPTION hair (99), 5 o la_ b- b_ a-9X3125 3 2411768521 88884444448888Θ84 TGAAGACTCTCTCGGAAGAGATAGCGTTTTTCTGCAACCTACGGTCCCAGCAGAAAAACC tgaagactctctcggaagagatagcatctttctgcaacctgcggtcccagccgaaaaacc tgaagactctctcggaagagatagagtctttctgcaacataaggtcccagccgaaaaacc tgaagactctctcggaagagatagcgtctttctgcaacataaggtcccagccgaaaaacc TGAAGACTCTCTCGGAAGGGATAGCGTCTTTCTGCAACCTGTGGTCCCAGCAGACAAACC tgaagactctctcggaagggatagcgtctttctgcaacctgcggtcccagcagaaaaacc TGAAGACTCTCTCGG-TGAAGACTCTCTO TGAAGACTCTCTO TGAAGACTCTCTCGGAAGAGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGAAAAACC tgaagactctctcggaagagatagcgtcttgctgcaacctgcggtcccagcagaaaaacc tgaagactctcttggaagagatagcgtcttgctgcaacctgcggtcccagcagaaaaacc tGAAGACTCTCTCGGAAGAGATAGCGTCTTGCTGCAACCTGCGGTCCCAGCAGAAAAACC tgaagactctctcggaagagatagcgtcttgctgcaacctgcggtcccagcagaaaaacc tgaagact Ctcttggaagagatagcgtcttgctgcaacctgcggtcccagcagaaaaacc TGAAGACTC----GGAAGAGATAGCGTCTTTCTCCAACCTGCGGTCCCAGCCGAAAAACC ccatttcctaatattatccttccctcttcctcctcagcaactagtcttctaaattagaac ★ « * * * *会食 *会* * « *

CGGAAGGGATAGCGTCTTTCTGCAACCTGCGGTCCCAGCAGAAAAACC cgg ^ gggatagcgtctttctgcaacctgcggtcccagcagaaaaacc &quot; GGAAGGGATAGCGTCTTrCTGCAACCTGCGGTCCCAGCAGACAAACC 1975 1975 2245 2255 1953 1975 1975 1975 1975 1975 1975 1975 1975 1975 1975 1975 1979 20 2530 35 88884444448888884 o la_ b- b_ 9131253241176852 45 Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 5055 o la- b - b- a_ 91312532411768521 68884444448888884 TTGTGATCCTTGTTGCGGGCGACATG---------------------------------- 2001 CTGTGATCCTTGTTCCGGGCGACATG------- --------------------------- 2001 TTGTGATCCTTGTTCCGGGCGACATG--------------------- ------------- 2271 TTGTGATCCTTGTTCCGGGCGACATG--------------------- 2281 TTGTGATCCTTGTTCCAGTCGACATGGAGGACGACTCACTCTACTTGGGAGGTGAGTGGC 2013 TTGTGATCCTTGTTCCAGTCGACATG---------- ------------------------ 2001 TTGTGATCCTCGTTCCAGTCGACATG------------------------ ---------- 2001 TTGTGATCCTCGTTCCAGTCGACATG--------------------- 2001 TTGTGATCCTTGTTCCAGTCGACATG--------------- ------------------- 2001 TTGTGATCCTTGTTCCAGTCGACATG- --------------------------------- 2001 TTGTGATCCTTGTTGCGGGCGACATG-------:....... .................... 2001 TTGTGATCCTTGTTGCGGGCGACATG---------------------------- ------ 2001 TTGTGATCCTTGTTGCGGGCGACATG---------------------------------- 2001 TTGTGATCCTTGTTGCGGGCGACATG------ ---------------------------- 2001 TTGTGATCCTTGTTGCGGGCGACATG-------------------- -------------- 2001 TTGTGATCCTTOTTCCGGGCGACATG------------------ 2001 TTAAACACAATG----AGCAGATATG--------- ------------------------- 2001 k # * * * * *** AGTTOU^CACTTTTCAAAACTCACATCTTCTCGGCCAGATGCAGCrrrrGCTGAAATCC 2073 101. This paper scale applies to Chinese national standards ( CNS) A4 specification (210 X 297 mm) 1312008 A7 B7 V. Invention description (100 Ministry of Economic Affairs Intellectual Property Bureau employee consumption cooperative printed 6 9 8 10 Θ 3 8 11 5 4 2_a 4 5 4 3 4 2 a b 4 4 10 4 1-b 8 1 8 7 8 6 8 8 15 β 5 8 2 4 l_a 20 β 9 8 10 8 8 ύ 11 4 2 a a 25 4 5 4 3 4 2_b 4 4 4 30 8 1 8 7 θ 6 8 8 3 5 35 8 2 4 l^a 3 9 40 8 10 8 3 8 11 4 2—a 4 5 45 4 3 4 2_b 4 4 4 1—b δ 1 50 β 7 8 6 8 8 8 5 8 2 55 4 1-a 60 65 AGCGGACTTCTCTCCCTGAGAAGTCACCACTCTCATGTGAGACCCGTGTCGACCTCTGAG 2133 ATGATTTGGCTCCTGTGGCAAGACAGCTTGCTCCCAGGGAGAAGCTTCCTCTGAGTAGCA 2193 GGAQACCTGCTGCGGTGGaCSGCTGGGCTCCAGAATATG 2231 Starting ATG HIXJB4 .6 putative promoter sequence upstream of gcatgactgg gcacaccgat ctcggcatct ctccctgtgg ggcacaggaa gggccaaagg ggccctgaag cagacagctt gcccaggtcc gcattgctca agcatctttc gccttctgat tcacagtcct ccagggctgt atcgattggg cggctgcagg tgcttctggg taagcagtgc ggagcacacc ctcatcccat ttctttacac ctcccctcaa aataagaccc gatcattctc Ccttttcttc tggcccatga catcctcctt ctagaggcct aatcggttat tccagggtct ctgaaaatgg ccccaggaca aaagacaagg aaaatcatcc tggcgccggg gagcattcaa tgtgtgaagc tggctccttt ctttacatcc gaaagaaacg taatttcatg cctcaattcc - 102- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention description (101 10 15 20 25 gccctgttcc ctgctaagcc tgtccacttt acaatgcgca caggatatgc ggcacgctat cagg Gcctca tccaaattag ggaaatatta aacaaacggt taaactgccg ctggcaaaca ccaaaggctt tctaccattt gacttgtctt cgtggaaagg ggaagaaaat cactttcgac acttggaagt cacgaacaca gccattgctt ctattatatg tgtgggctgt tgaattgagc gcggttggag actctctcgg atccttgttc ttaccgtcta agacagccgg

cggcagcatC actggaaatg acggcctatc cctctgtttc cttccgtcag aactttttat tcacacatgc tatagctggg tcgcacgtcc taaatttggg tcacagtcta catgctttct gttctccact ttatcgctgg acgctgtgct ttcctgtctt tcatcgcaga atcacacaca taoctaaaat tgcctatcat gtctgttgaa gctgatattt ggggtgactt aagggatagc cagtcgacat agacatgttg aagacacacc acaattttgg gaggcaacat: tcggcctttt actccctgct ccgggcttCt ttcacctcta tgtgagatac ataccttctg gaaaccgctc gattgtcatc

CtttgtgcAC tacttggcaa ggcaggtaat aaggtctgtt aaatactata tccgtcgct aaaaacacac cacacactca aaggcaactg cctgaggagt

Ctcccagcga gattggctta acagaagggc gtcttcctgc g ggaaaatccc caaatcctgt caccaaatgt ctccgatcct cccactgaaa tc.taaacgtc ccacggtata ggaaacgttg gttgtttcta agttctcaag actatgcagt aattagtcCc tgagtatctc tctagcttcc ttcactcaga tgattccacg cttcattgac agaggatgat acacacacgc cacggtttcc tgtggccact aatttgattc tgcaagggga tcgcgcacct tgatgcggcc aacctgcggt tagagccagg ccctcttact gctaactgca gaacgatcga caagggccag tccgacgttt attttccttg atccattatc ttttcatcaa tttcttgttt gtcatgaccg tcggggattg ttcaaacttc acaagagcat tagagaatca gatctctcct tattctcagg ggcagctgcc gcccccccca tacgtaaaga gtcccaaccc aggtgttctg cacaccctgt gatgagtggg agagagctcg cccagcagaa atcttcattc tcagggaaca attccaccat tgcgagaatc tattaaaaat ctccctgaga tttgcttttg acatacgtat ttctttaata cgtgttttcc tctctctttc cacgatttcc agtgcatgtt tCcaCgcaaa ataggctcaa ttctcatcag tcagaaagcg aaaagtacat cacacacaca tttcttccct ggttacactc gaagtcatgc gactccttcc tgtggtgttc tcatttgaag aaaccttgtg 30 HDUB4.7 ATG initiation of putative promoter sequence upstream of the enemy tcctcagcgt Cggtttttag gcctggcata ccatcatctt t ggcctagtt aacacctccc cccactgatc ccaaacccag cctaatggag atttaatgcc attaaatcag cattcactta catacttgca gagtcggcca actttgggaa gccgaggtgg gtggatcacg catggtgaaa ccccgtctct actaaaaata tgtaatccca gctactcagg aggctgaggc gttgcagtga gccaagatta tgccattgca agaaaacaaa aaacccaaaa acttgcagag tgatccaatg ccttcctttt tctctttctc tgtcctgtga tatttacaat gttctgagat gtctgctgtg tgctggaaag caggtcttgg attggttggc cagaagccca tccctccctc cctccctccc ggctctgctg gctgccaaga agaagagata agaaattcca Order Ministry of Economic Affairs Intellectual Property Office staff ttttcctcat consumer cooperatives printed 35 40 45 50 55 60 tttcacgcct tccacaagag ggatagagaa acggatctct gactattctc gatggcagct cacgcccccc taggtaaaga gtcccaaccc aggtgttctg cacaccctgt gatgagtggg gtaatcccag catttcacgc tcaataggct cctttctcat aggtcagaaa gccaaaagta cacacacaca tttcttccct ggttacgctc gaagtcatgt gactccttcc tggggtgttc ctaggagaag tttgcaggta cgctatattt atcgtcactc tctccaagat tgggccacca cagggctgat cacttgggag aaagacttgt caacgtggaa tagggaagaa gcgcactttc catacCtgga cacacaaaca gccattgctt ctattatatg Tgtgggctgt tga attgagc gcggttggtg agctgtttga ctccgtgtgt gacaccctac aagaaattct ggcgtgttgg aggtcaagaa caaaaactaa aggcgatttg ctccagcctg tgaatttagg aaatattttg gcaggagaga ccttccctca ataccatggt tctctctctg atccagtaac gcaaaaaggg aagaaagacc gccaggatgg cttgttctcc aggttatcgc aatacgctgt gacttcttgt agttcatccc cactcacaca tacctaaaat tgcctatcat gtctgttgaa gctgacattt ggggtgactt aacccaggaa ggtggtttgg agctgctgac gattctcagg ctcacacctg ttcgagacca ctggcgtagc cttgaacctg ggcaacagag aaaccatgaa agccaggtac gaactaagaa atttccctac acttcccctc tctccctctc tgactgatca aaaattggtt atgggcttcc tcggatttgg actggcaggt tggaaggtct gctaaatact ccttccgtcg agcacaaaca cacacacgca aaggcaactg cctgaggagt ttcccagcga gattggctta acagaagggc cgtaccccac agaacctgct ca ^ gattaaa gactgacatC taatcccagc tcctggccaa tgtgcgtgcc ggaggtggag cgagactctc gtacacagtt tatcctagac cagagagcat tcactggcca cctatactca gctccctccc gctgggggtg gtgagtgaga aggcgcggtg caatctagct aattCcactc gtttaactcc atacttcatt ctgagaggat cacacacaca cacggtttcc tgaggccact aatttgattc tgccagggga tcgcgcacct tg Atgcggcc This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) 1312008 A7B7 V. Invention description (l〇2 agagagctcg tcatctgaag actctctcgg aagggatagc gtccttctgc aacctgcggt cccagcagac aaaccccgtg atccctgttc cagtcgacat g Start ATG HDUB4.S presumption start Subsequence upstream 10 15 20 25 30 35 40

CAGGGCTCCG TACGAACAGG GACAGCTTAT CCAGGTCCCG ATtGCTCATG CATCTTTCTA CTTCTGATGG ACAGTCCTCT AGGGCTGTTT ACCGTCTAAG ACAGCCGGAA GCAGCATTAC TGGAAATGGA GGCTTATTTT TCTGTTTCAC TCCGTCAGCC CTTTTTATTT ACACATGCTG TAGCTGGGAT GCACGTCCGA AATTTGGGGA ACAGTCTACT TGCTTTATTA TCTCCACTGG ATCGCTGGAA GCTGTGCTAA CTTGTCCTTC ATCCCAGCAC CACACACACA TAAAATAAGG TATCATCCTG GTTGAATACC ATATTTGATT TGACTTACAG

TAGAACCACA GAATCTTGGG CGCAACCCTG CTCAAGCACC CAAATGTGCA GTCTCCGTGT GACGTGTOTG AAAACTACAG TGTGATGAGC ATGACTGGCA CGATTGGGCT CCCCTCAAAA TCGGTTATGA GCATTCAAGC ACACCGATGC GCTGCAGGAA TAAGACCCTC CAGGGTCTTG TGTGAAGCCT CGGCATCTGC CTTCTGGGGA lOA ^ rTCTCCT GAAAATGGTG GCTCCTTTCT CCCTGTGGAG AGCAGTGCTC TTTTCTTCCC CCAGGACACT TTACATCCGG CACAGGAAGC AGCACACCTG GCCCATGAAA AGACAAGGGA AAGAAACGGG GCCAAAGGTC

CATCCCATCA TCCTCCTTAA AATCATCCTA CTTTACACCT AGAGGCCTTG GCGCCGGGCC ACATGTTGGG AAAATCCCTA GAGCCAGGAT GACACACCCA AATTCTGTCC CTCTTACTTC AATTTTGGCA CCAAATGTGC TAACTGCAAT GGCAACATCT CCGATCCTGA ACGATCGATG GGCCTTTTCC CACTGAAACA AGGGCCAGTA TCCCTGCTTT TAAACGTCTC CGATGTTTCT CCCTGAGACA GGGCTTTTCT ACGGTATAAT TTTCC CACCTCTAGG AAACGTTGAT CCATTATCAC ATACGTATGG AAATATTATC TGAGATACGT TGTTTTTATT TTCATCAATT CTTTAATAAA CAAACGGTTA ACCTTCTGAG TTCTCAAGTT TTTTGTTTCG TGTTTTCTTA AACTGCCGTC AACCGCTCAC TATOCAGTGT CATGACCGTC TCTCTTTTCT GGCAAACATA TTGTCATCAA TTAGTCTCTC GGGGATTGCA TGATTTCCCC AAAGGCTTTC TTGTGCACTG AGTATCTCTT CAAACTTCAG TGCA1X5TTTC TACCATTTGA TTTGGCAATC TAGCTTCCAC AAGAGCATTT CATGCAAAGA CTTGTCTTCT CAGGTAATTT CACTTGGACA GAGAATCAAT AGGCTCAACG TGGAAAGGTT GGTCTGTTTG ATTCCACGGA TCTCTCCTTT CTCATTAGGG AAGAAAATAC ATACTATACT TCATTGACTA TTCTCAGGTC AGAAAGCGCA CTTTCGACTT CGTCGCTGAG AGGATGATGG CAGCTGCCAA AAGTACATAC TTGGAGGTTC AAACACACAC ACACACGCGC CCCCCCCACA CACACACACA CGAACACAAT CACTCACACG GTTTCCTACG TAAAGATTTC TTC CCTGCCA TTGCrTTACC CAACTCTGTG GCCACTGTCC CAACCCGGTT ACACTCCTAT TATATC?TGCC AGGAGTAATT TGATTCAGGT GTTCTGGAAG TCATGCTGTG GGCTGTGTCT CAGCGATGCA AGGGGACACA CCCTGTGACT CCTTCCTGAA TTGAGTGCTG GGCTTATCGC GCACCTGATG AGTGGGTGGG GTGTTCGCGG TTGGTGGGGG AAGGGCTGAT G 45 Start ATG HDUB4,9 Presumptive Enemy Sequence

iAAGCC CGC CCTGTTCCTT: CT GCTAAGCCAG AGGGAACATG TCCACTTTCG AC AATGCGTAAC .TCCA GGATATGCAC .TGG CACGCTATCC Order Ministry of Economic Affairs Intellectual Property Office employees consumer cooperatives printed 50 55 gcatctttcc ccttctgatg cacagtcctc agggctgttt accgtctaag acagccggaa gcagcattac tggaaatgga ggcttatttt tctgtttcac tccgtcagcc ctctttattt acacatgctg agtcagcgct gagcacacct tcctcccatc ctttacacct acatgctggg gsLcacaccca aattttggca ggcaacatct ggccttttcc tccctgcttt gggcttttct cacctctagg tgagatacgt cttttctCcg ggcccatgaa atcctcctta agaggccttg jaaaatcccta aattctgtcc cca &amp; atgtgc ccgatcctga taaacgtctc acggtataat aaacgttgat tgtttttact cccaggacac aagccaaggg aaatcatcct gcgccgggcc gagccaggat ctcttacttc taactgcaat acgatcgatg agggccagta cgatgttgct tttccttgtc ccattatcac ttcaccaatt tttacatccg aaagaaacgg aatttcctgg tcaattccgc cttcattcct agggaacatg tccacqatac cgagaatcca tta atacgtatgg in aaatgg ccctgagaca tgcttttgtc ctttaataaa gcacacgaag ggccaaaggt ccctgaagcc Cctgttcctt gctaagccagtccactttcg aatgcctaac ggatatgcac cacgctatcc ggacctcact caaattaga a aaatattatc caaaaggtta -104- This paper size applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention description (i〇3 15 cagctgggat gcacgtccga aatttgggga acagcctact tgctttatta cctccaccgg ttcgctggaa actgtgctaa cttgcccttc atcccagcac acacacacaa aacaaggcaa catcctgagg Gaattcccag actcgattgg acttacagaa tagcgtcttt acatg accttctgag aaccgctcac ctgtcaccaa

Ctgtgcactg ctcggcaatc caggtacttt ggtctgtttg atactatact cgtcgctgag aaacacacac tcacacggtc ctgtgtgacc agtaatttga cgatgccagg cttatcgcgc gggctgatgc ctgcaacctg ccctcaagtt

Catgcagtgt tcagtctctc agtatctctt tagcttccac cactcggaca accccacgga tcattgacta aggatgatgg acacgcgccc tcccaggtaa actgtcccaa tccaggtgtt gggacacacc acctgatgag ggccagagag cggtcccagc ttttgtttcg catgaccgtc ggggattgca caaacctcag aagagcattt

gagaaCcaaC tctctccctt tcctcaggtc cagctgccaa ccccacacac agatttcttc cccggtCaca ctggaagtca ctgtgactcc tgggtggggt ctcgtcatcc agaaaaacct tctcttttct tgaCCtcccc tgcatgtttc catgcaaaga aggctcaacg ctcattaggg agaaagcgca aagtacatac acacacaaac cctgccatgg ctcctattat tgctgtggga ttcctgaatt gttcgcggtt gaasraccctc tgtgatcctt aactgccgtc ggcaaacata aaaggccccc tacaatttga cttgtcttgt tggaaaggtt aagaaaatac ctttcgactt ttggaagttc acaaccacac ctttacctaa atgtgcccat tgtgcctgtt gagtgctgat ggtgggggtg tcggaaggga gttccagtcg 20 of the start ATG HDUB4.10 putative promoter sequence upstream 25 agcaagcttt gtggtgtttg aggtcctcat atgtgcaata ctcttgtcta attacttctc tcaccgccgc agaagagtga ttggcgcaga gcctccaggc tagaccactg aagtcaaagg ggatgaagaa cgacacatca gatccaaagg ggaagtaccg aactacaccc aatcctacat agtgatggct agtcttccga gtgtgtattt gactcggttt gaaggtgaaa gaaactggaa tttttacact ccacataacg agtccaaacg aataaaggaa gaaatcaatc ccagctgcga ccgacgtggt ctgattagac gaggaaagct ccctgctcaa ggaacagttg tctccagagg ccttgtattg tcctgagtgc tgtcctctat ttatgtcaaa tagcatcact atgggaaaga agacacagac ccccgagttg gaaattcctt taccctgcct ccatcatcct ggagtccatg gaagaacaaa acccacacgt agaagcgcgc aaactaatga acatctcagg acctctggag ctgaatatga cgtattttct tgtggctcat tgcatttgga ggttcctgaa ccggaagcgg gaaaattttc agcaaacaca tcactgggtg aagctgcatc gtttccgtgg ttcatggtcc gcccagggca gcacccaaat gtgaagcccg gcgccggcct aagagattct cttgacatgc gctgtgctgg gctcaagaag tctgtcctga catagtgaga aggcgagcaa gacgagcact caagagcaaa cccgacgtac gaacagcaaa aacactggca cacagcaaga aggggtgcac acgcaaacac ggagcccaag atgagcccgc ggactgtctg cctaccgacg cttggggtgt gtgggacatc agagaagaac tggacctcag acagaccgac ccacgggcat ggagtgtgtg tgtttaaaaa tctgaagcac aaatgattgt ctttctcgtc caactcacrggg aagaactcaa ccaagacgtt ccgatgtcac agccatacat tccacgctga gccagtggta gtcaacaggc gtgtgtcaag cgcaaggaga tggtggaaag acaaaacgaa ttgtgattca gctccctgct cactcgcttc gggctctgct acacacacgc acacacaccc tttctgtctc atatgggaaa agt ^ tttgtg tgtaggtttg gtttcattcg cgtggatcat ggtgctcttc gcgccctggg ttgcctgttt gtaagtcatc tattcaactg tggcatttgg agtccctgtc gggaaat ggc aggtacagtg ctccgtagaa gcctatcatt acctctgcca attgccaaga aacacaggac cacaacggac gatgccgag? ttttacatcc ccaagagccc gaccacccct gaaagcacct aacgtcagaa tacaagtgtg tcgtcgaccc agggcca ^ ga tgatctcagt acacacacat gaacaccgtc acaactggat tggggtcgtg gtgacattca aattgcaggg gtcggcatga ttgaatagtg gggctcgccg ttgctggaac gcttcccatg ctgtattgat agaaagccct ggtcatttgt acttattgat tctctatttg accagcccca ttgggcgcaa tggagagaat aactttacac aggcaacaag gtctcagcag gtggagttgt taaaatggat ctacgtcctc aggcagggaa gctcaagaga agccactcag gcctgagttc tcaatcaaaa aaacctctct cctgcgaggg tgtgtgccag acacacacag acacaaacac tacaacaggg catcaagttt ttcatgata? cgtgtgaggt tcagttgttg tctcgccacc tttcttcccc acttgtgctc cacggtgccc tggaagtaag aaataaattc ggaatttctg ccgcagtgag cccttccttt atcaaagtt? ccacagaatc Order Ministry of economic Affairs intellectual property Office employees consumer cooperatives printed HDUB4.11 30 35 40 45 50 55 ATG initiation of the putative promoter Upstream subsequence 105- This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) A7 B7 1312008 V. Invention description (1〇4 10 15 25 30 cagcaagctt tgtggtgttt aaggtcctca aatgtgcaat cctctcgtct cattactcct gtcaccgcct cagaagagtg cttggcgtag tgcctccagg ttagaccact agagtcgaag cggatgaaga ccgacagatc agatccaaag tggaagtacc taactacacc caatcctaca tagagatggc gagtcttccg agtgtgtatt ggactcggtt agaaggtgaa ggaaactgga gtttttacac cccacataac gagtccaaac taataaagga tgaaatcaat tccagctgcg tccgacgtgg gctgactaga agaggaaagc accctgctca tggaacagtt gtctccagag tccttgtatt atcctgagtg atgtcctcta cttatgccaa ctagcatcac aatgggaaag aagacacaga cccccgagtt ggaaattcct gtacggtgcc accatcatcc aggagtccat ggaagaacaa gacccacacg cagaagcgcg taaactaatg tacatctcag aacctctgga tatgaatatg tcgtattttc atttggctca atgcatttgg tagttcctga gccggaagca ggaaaatttt aagcaaacac ctcactgggt aaagctgcat tgtttccgtg cttcatggtc tgcccagggc agcacccaaa ggtgaagccc ggcgccggcc gaagagattc ccttgacatg tgctgtgctg agctcaagaa ttctgtcctg acacagtgag caggcgagca ggacgagcac tcaagagcaa tcccgacgta tgaacagcaa gaacactggc acacagcaag taggggtgca cacgcaaaca asgagcccaa gatgagcccg gggactgtct acctaccgac tcttggggtg tgtg ggacat aagagaagaa atggacctca gacagaccga cccacgggca aggagtgtgt gtgtttaaaa ctctgaagca gaaatgattg cctttctcgt acaactcagg tg gaagaactca tccaagacgt cccgatgtca cagccataca gtccacgctg ggccagtggc agtcaacagg agtgtgtcaa acgcaaggag ttggtggaaa aacaaaacga cttgtgattc agctccctgc acactcgctt agggctctgc tacacacaca cacacacacc gtttctgtct catatgggaa gagtgtttgt gtgtaggttt tgtttcattc ccgtggatca cggtgctctt ggcgccctgg cttgcctgtt tgtaagtcat gtattcaaca atggcatttg cagtccctgt tgggaaatgg caggtacagt gctccataga atggagagaa taactttaca caggcaacaa tgtctcagca ggtggagttg ataaaatgga cctacgtcct gaggcaggga agctcaagag gagccactca agcctgagtt atcaatcaaa taaacctctc ccctacgagg ttgtgtgcca cacacacaca cacacaaaca gtacaacagg acatcaagtt gttcatgata gcgtgtgagg gacagttgtt ttctcgccac ctttcttccc gacttgtgct tcacggtgcc ctggaagtaa gaaataaatt gggaatttct cccgcagtga ccccttcctt gatcaaagtt accacagaat tgcctatcat caactctgcc aattgccaag gaacacagga tcacaacgga tgatgccgag cttttacatc accaagagcc agaccacccc ggaaagcacc caacgtcaga atacaagtgt ttcgacgacc gaggaccagg Gtgatctcag cacacacaca cgaacaccgt gacaactgga ttggggtcgt ggtgacattc taattgcagg ggtcggcacg cttgaatagt cgggctcgcc cttgctggaa cgcttcccat gctgtattga cagaaagccc gggtcatttg gacttattta ttctctattt gaccaacccc cttgggagca Simple illustration of the diagram Figure 1 shows the flow of the proposed DUB-enzyme reaction mechanism

Printed by the Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperatives 6 ο This paper scale applies to the Chinese National Standard (CNS) A4 specification (210x297 mm)

Claims (1)

τ *月尔Cv—η ROC Patent Appln. Νο.92103585 * Repair|e part unlined patent application scope Chinese replacement page-attachment (II) i Amended Claims in Chinese - Enel, ill) Benefit range (Republic of China 97 years) Submitted on September 19) (Submitted on September 19, 2008) 10 15 20 1 An isolated polynucleic acid whose coding is selected from the group consisting of sequence identification number: 3 (hDUB 4.1a), sequence identification number: 5 (hDUB 4 .lb), sequence identification number: 7 (hDUB4.2a), sequence identification number: 9 (hDUB4.2b), sequence identification number: (hDUB4.3), sequence identification number: 39 (hDUB4.4), sequence identification number :丨3 (hDUB4.5), sequence identification number: 15 (hDUB4.6), sequence identification number: 17 (hDUB4.7), sequence identification number: 19 (hDUB4·8), sequence identification number: 41 (hDUB4. 9), sequence identification number: 21 (hDUB4.10), sequence identification number: 23 (hDUB4.11), sequence identification number: 25 (hDUB8.1), sequence identification number: 43 (hDUB8.2), sequence identification number :27 (h〇UB8.3), sequence identification number: 29 (hDUB8.5), sequence identification number: 31 (hDUB8·6), sequence identification number: 33 (hDUB8.7 ), sequence identification number: 35 (hDUB8.8), sequence identification number: 45 (hDUB8.9), sequence identification number: 47 (hDUB8.1〇), and sequence identification number: 37 (hDUB8.ll) humans go Pan-hormone protease. 2. A polypeptide having a selectivity selected from the group consisting of a sequence identification number 4 4.1a), a sequence identifier number: 6 (hDUB 4.1b), a sequence identifier number: 8 (hDUB 4.2a), a sequence identifier number: 10 (hDUB 4 , 2b), sequence identification number: 12 (HDUB4.3), sequence identification number: 4〇 (hDUB4.4), sequence identification number: 14 (hDUB4.5), sequence identification number: 16 (hDUB4.6), sequence Identification number: 18 (hDUB4.7), sequence identification number: 20 (hDUB4.8), sequence identification number: 42 (hDUB4.9), sequence identification number: 22 (hDUB4.10), sequence identification paper size for China National Standard (CNS) A4 Specification (210x297 public) 92083-claims-Riding Ordering Line 1312008 cs _____ D8 VI. Patent Application No.: 24 (hDUB4.ll), Sequence Identification Number: 26 (hDUB8.1), Sequence Identification number: 44 (hDUB8.2), sequence identification number: 28 (hDUB8.3), sequence identification number: 30 (hDUB8.5), sequence identification number: 32 (hDUB8.6), sequence identification number: 34 5 ( hDUB8.7), sequence identification number: 36 (hDUB8.8), sequence identification number: 46 (hDUB8.9), sequence identification number: 48 (hDUB8.10), And the sequence identification number: 38 (hDUB8.11) of the human amino acid sequence of the ubiquitinated protease. 3. A method for identifying an inhibitor of hDUB of claim 1 in the method of assay, which uses a polynucleotide as claimed in claim 1. 4. A method for identifying an inhibitor of hDUB of claim 2 in the assay, which uses a polypeptide as claimed in claim 2. 15 5. A pharmaceutical composition for attenuating inflammation by signaling a primary inflammatory cytokine, comprising a compound which inhibits a polypeptide as claimed in claim 2 of the patent application. Printed by the Intellectual Property Office of the Ministry of Economic Affairs, the Consumers' Cooperatives. 6. A pharmaceutical composition that regulates autoimmune diseases by altering the cytokine receptors involved in lymphocyte proliferation, and contains the second component of the patent scope. a compound of a polypeptide. A pharmaceutical composition for modulating an immune response during infection, which comprises a compound which inhibits the polypeptide of claim 2 of the patent application. 8. A pharmaceutical composition for attenuating inflammation by regulating primary inflammatory cytokine signaling, which comprises a compound capable of altering the polynuclear buds of the patent range i. A pharmaceutical composition for modulating an autoimmune disease by altering a cytokine receptor signaling involved in lymphocyte proliferation, comprising a compound which modifies transcriptional regulation of a polynucleotide according to claim 1 of the patent application. A pharmaceutical composition for modulating an immune response during infection, which comprises a compound which modifies transcriptional regulation of a polynucleotide as in claim 1 of the patent application. 11. A method of identifying a human ubiquitin-promoting protease modulator, wherein the compound is added to the timidally linked to the reporter gene, comprising a sequence identification number: 3 (hDUB 4.1a), a sequence identification number :5 (hDUB 4.1b), sequence identification number: 7 (hDUB4.2a), sequence identification number: 9 (hDUB4.2b), sequence identification number: 11 (hDUB4.3), serial number ij identification number: 39 (hDUB4 , 4), sequence identification another ij number: 13 (hDUB4.5), sequence identification number: 15 (hDUB4.6), 15 sequence identification number: 17 (hDUB4.7), sequence identification number: 19 Ministry of Economic Affairs Intellectual Property Bureau Employee consumption cooperative printing (hDUB4.8), sequence identification number: 41 (hDUB4.9), sequence identification ij number: 21 (hDUB4.1〇), sequence identification number: 23 (hDUB4.ll), sequence identification number :25 (hDUB8.1), sequence identification number: 43 (hDUB8.2), sequence identification number: 27 (hDUB8.3), 20 sequence identification number: 29 (hDUB8.5), sequence identification number: 31 (hDUB8. 6), sequence identification number: 33 (hDUB8.7), serial number identification number: 35 (hDUB8.8), sequence identification number: 45 (hDUB8.9), Column identification number: 47 (hDUB8.10), and sequence identification number: 37 (hDUB8.11) Human deubiquitinated protease 5' end polynucleotide This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm) B8 C8 D8 1312008 6. In the report of the patent application scope, the effectiveness of the compound is determined. Ministry of Economic Affairs, Intellectual Property Bureau, Staff Consumer Cooperatives, Printing and Clothing 4 This paper scale applies to China National Standard (CNS) A4 specification (210 X 297 mm)