TW390906B - Apparatus for biomass production - Google Patents

Description

Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. 5. Description of the Invention (i) :: It is a device for biomass production. This device is a method for cultivating biomass. The self-operated use of dr and the generation, treatment, and accumulation of Qiongbaiguan == long-term potential has gradually become apparent. Self-supporting organisms-species that obtain two from the sun through photosynthesis (photo-self-supporting organisms) with heart-&gt; organic or inorganic reduced chemical compounds (chemical self-supporting organisms). Typical examples of this include plants, seaweed , Lotus culture ^ bath: spirulina, Dunaliella, etc.), sialomyces, photosynthetic bacteria and so on. Based on the requirements of breadth, self-supporting organisms are excellently suitable for producing biomass. Bath cultures are taken as an example. 'It has been cultivated for food for animals and humans. ("Water and calendar treatment, for radioactive waste Accumulation, for the production of enzymes and two industrial and research applications for the proliferation of products, and can produce oil and other nutritious products at high prices. The traditional step I of the cultivation of self-operating organisms involves the use of exposure to light < Shallow water in vast pools or channels. These rather light, self-cultivating organisms have proven impractical because they are contaminated by dust, microorganisms, insects, other animals, and environmental pollutants, and are being controlled. The degree of exposure, temperature, respiration, and other factors are limited.-An attempt to overcome these problems involves the use of a bioreactor system, which is wrapped in a relatively small diameter light transmitting tube, and is essentially horizontal. It is placed on the surface of a series of flat vertical plates. The difficulty associated with this type of bioreactor is the low-density biomass in the liquid in the tube, and the light penetration is low. Low speed causes self-supporting organisms to coat the pressure pipe wall and therefore reduce transparency, which will overheat in summer weather and a large number of this type when operating commercial production systems. -4- 'Paper size applies to Chinese National Standard (CNS) A4 specifications (210X297mm) (#Read the precautions on the back before filling in this page)

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5. Description of the invention (2) The unit will have high land use and process control issues. Furthermore, the bioreactor is designed to have a traditionally unstable structure that cannot withstand climatic conditions. Another different configuration includes an upright core structure around it, which is wrapped with a substantially transparent tube, which allows at least 90% of the light to pass through, so that the outside of the tube wrapped during use can be exposed to natural light. In order to stimulate the light to penetrate inside, the upright core structure is equipped with light reflecting components. Via the coiled tube 'there is passed a synthetic mixture containing live plants such as algae, phage and seaweed, and growing essential nutrients. Similarly, this kind of bioreactor has self-supporting organisms coating the wall of the tube during use, it will overheat in summer, the overall construction cost, the volume of liquid that can pass through the tube at any time is limited, and it is difficult to sterilize the device between culture batches Wait for the innate difficulties. T-shirts printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 丨 〈Read the ii meanings on the back of the ears first, the ears of the town '' a more complex structure is a bioreactor that can cultivate photosynthetic microorganisms, among which there are many adjustments The plate holder is on the barrel of the photobioreactor. The hollow tube cavity in the form of a regulating plate passes through the entire reactor, and the internal light source can be embedded through the opening in the barrel wall. Although this structure can maintain higher density of biomass in the culture liquid and reduce land use, the complexity of the bioreactor barrel, the cost of the overall construction, and overheating in the summer make this barrel more commercially available. Uneconomical. Furthermore, it is particularly difficult to clean and disinfect between operating cycles. The present invention proposes an improved bioreactor that can be manufactured at least in different sizes and lengths, and is suitable for commercial production of self-supporting organisms. Therefore, the present invention relates to a bioreactor for the production of biomass. The size of this paper applies to China National Standard (CNS) A4 (210X297 mm).

Including ·., (), A kind of room on the top of 'A', this room is at least suitable for containing a liquid phase I organism m and has a bottom, top and many side walls in between. Separated from the bottom to the top; and (u) — the contents of a circulation member circulation chamber, wherein the circulation member can generate a joint moving force within the phase to ensure that substantially all biomass can be continuously mixed, and at least Semi-continuous exposure of the biomass to the light source. There are many variables that can affect the production of biomass in a bioreactor according to the invention. One of them is the rate of light and biomass circulating through the cell. Better 'Small 1: The essence of being substantially transparent is transparent to light. When the biomass density in the bioreactor increases, the intensity of light penetrating the liquid phase is mostly reduced. Preferably, all biomass on the substrate can be exposed to light at least on a semi-continuous basis. Under certain conditions, biomass can be continuously exposed to light sources. The light source may be natural light such as sunlight, or it may be artificially generated. The circulation rate in the printing chamber of the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs should be set at an appropriate level to ensure that the liquid phase can be mixed in a continuous manner, and in this way, the biomass can be exposed to light during the cycle without being exposed to light. As a result, the biomass has an inappropriate pressure. It should be understood that certain changes in the configuration of the invention can still be achieved without departing from the invention. In general, the circulation system used in the present invention can keep substantially all of the biomass in the continuous release solution, and can avoid the biomass sink in the bioreactor. Preferably, all biomass can be maintained in a continuous suspension. The power required to generate a suitable circulation rate of the liquid phase in the bioreactor, _ -6- This paper size is applicable to the Chinese National Standard (CNS) A4 specification (2 丨 0X297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 ___—: _B7 ^ ____ 5. Description of the invention (4 {^ '~' 2) The size of the bioreactor, the biomass cultivated in the reactor and the density of the biomass are greatly related. The lowest biomass in the bioreactor The circulation rate is better than that of biomass but Shen Muxi Lenghui. Xi Jiu 'Li J Jiujue <The circulation rate of the lost hand is still lower than the rate of excessive foam development on the liquid surface in the bioreactor, and / Or the rate at which the biomass can receive excessive ring block pressure is still lower. The better circulation rate is set between the lowest circulation rate and the highest circulation rate. It is better to set the circulation rate close to the minimum circulation rate. For example, at an extended 35 The 0-liter trough-shaped prismatic plug bioreactor does not have an inverted, generally triangular cross-section, and can be circulated by releasing gas at or near the inverted apex of the bioreactor. Low gas feed is preferably 12 liters per minute per meter. Highest gas feed is preferably 20 liters per minute per meter, because the dynamics of the environment in the bioreactor will continuously change with increasing biomass concentration It is better to track the circulation rate and adjust it so that the circulation rate does not fall below the minimum circulation rate. It should be understood that the present invention can be used to produce various types and types of biomass or-products derived from them. So-called, "Biomass" as used herein includes all photosynthetic growth organisms, such as plant cells and microorganisms (including algae and euglena) that can grow in the liquid phase in a single-cell or multi-cell type. The term also includes artificial Modified or genetically manipulated organisms. The bioreactor of the present invention is particularly suitable for the cultivation of algae or photosynthetic bacteria. For example, the bioreactor can be used as a photobioreactor for producing algae biomass. In this aspect, it has special and various Various types of algae (such as chlorella, spirulina, Dunaliella, etc.) can be cultivated in bioreactors under such growth conditions. In order to make the biomass breathable, Phase needs to provide more than one member of the present paper scale applicable Chinese National Standard (CNS.) A4 size (210x 297 male thin) (read back all of the precautions again. Complete this page)

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mn-、 1T V. Description of the invention (5) A7 B7 = gas phase is introduced into the liquid phase. At this point, any life will not be removed (please read the precautions on the back before filling this page). Or use-more than one type can be opened into the bioreactor: guide. Through this delivery means, any gas required for photosynthetic activity can be introduced into the bioreactor. The preferred gas is a mixture of air carbon dioxide and oxygen. In order to prevent the biomass from being cold-stained in the reactor, the introduced krypton gas can be lightly sterilized to remove the doped pathogens before being released into the reactor. In a highly preferred form of the invention, the circulation of the liquid phase is achieved via a gas delivery system. It should be understood that the configuration of the circulation member can be in various types, and the restriction condition t can at least circulate the contents of the cell and provide ventilation for the liquid phase held in the cell. Furthermore, the circulation component can be used in the bioreactor-it is necessary to generate a suitable kinetic force in the liquid phase to ensure that the biomass remains substantially continuous and that the biomass is in contact with at least semi-continuous exposure of the light source. . ° Employees' Cooperatives Association of the Central Bureau of Standards, Ministry of Economic Affairs, 11 Better circulation components are located near the base part or near the top of the bioreactor above the base part. For example, when the circulation member is adjacent to the portion of the substrate within the bioreactor ', it can often cause upward movement from the substrate to the top of the bioreactor. The upward movement of the liquid phase and biomass can impact the upper surface of the container, and can temporarily ensure that light can be exposed to most of the biomass in the liquid phase. After coming into contact with the upper surface of the biological reaction, the liquid phase containing the biomass can be moved outward to the side of the bioreactor. This movement also ensures that biomass in the liquid phase can be exposed to light. The side of the liquid-phase bioreactor containing the bio-reactor moves down to the base part, whereupon it is captured again by the circulation component and driven up to the top of the bioreactor. This mixing process contributes to the rapid and consistent photo-synthetic growth of biomass, because 8-Paper scale is applicable to Chinese National Standard (CNS) A4 specification (210X 297 mm) A7 _____ __B7 ......... 1 —_ V. Explanation of the invention (6) It can ensure that substantially all biomass and light in the liquid phase are effectively exposed, and it also causes a regular “flash” light pattern, which has been shown to strengthen certain photo-synthetic organisms. Grow. Che Hao Bioreactor has a surface area to volume ratio. To achieve this, almost the entire surface of the biological reaction device is used as an active light absorbing / transmitting surface. The absorption and transmission of light can be further enhanced by using transparent structural substances in the structure of the bioreactor and by slightly suspending the surface of the bioreactor on some reflective substrates. Preferably, the formed bioreactor is an elongated trough-shaped prism, and the prism has an inverted triangular cross section (generally speaking). The sides that are generally triangular in cross section are preferably approximately the same, and the inverted apex can define the base portion of the cell. Furthermore, the circulation member is a capping member located at or near the inverted apex, and the side of the prism body can define side walls and cells. Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs. When the bioreactor is in the form of an elongated trough-shaped prism, the liquid phase can be introduced into the circulation when the liquid phase is moved from one end of the bioreactor. The other end is part of the biomass treatment process. Such movement can also avoid the local capture of Biological I. If this happens, it will affect the exposure process of biological response. Transferring the liquid phase in this way can also achieve complete mixing, as well as chemical and physical operations to transfer the biomass liquid phase environment. The circulation component can also extend in the form of a duct through the length of the chamber, which is located at or near the base of the chamber. The duct can be perforated and connected to the source of the gas. In this way, the gas can come out of the duct and enter the chamber under pressure, resulting in a small chamber. A content cycle. Gas is passed through the conduit. It also provides a method for diffusing gas into the liquid phase through the liquid phase, thereby venting the phase. Better here -9-This paper size applies Chinese National Standard (CNS) A4 specification '(2i0x297 Gongchu) A7 B7 V. Description of the invention (7) The gas in the type is preferably sterile air. In the better type, the holes are arranged below the duct, so that the gas passing upwards helps the particulate matter in the small chamber not to deposit under the duct. Therefore, in a preferred form, the present invention relates to a bioreactor capable of producing biomass, including: (i) a substantially transparent cell, the cell is at least suitable for containing organisms in a liquid phase, and has one A narrow base portion, one of which can be an upper portion sealed by the addition member and a plurality of side walls separated upward from the base to the upper portion; and (ii) a circulation member that can circulate the contents of the chamber, in which the circulation member is produced. Generate a suitable kinetic force to ensure continuous mixing of the liquid phase and expose the biomass and light, where the circulation component is located at the bottom or near the base portion and includes a conduit along the length of the bioreactor. The conduit has Perforate and connect to the gas source body, where the perforation faces the bottom end of the duct. In this way, the gas will exit the duct and enter the liquid under operation. Circulation; and (c) allowing the gas to diffuse into the liquid phase. Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page). The small room can be divided into transparent material sheets and connected together to form the bottom, side walls and cover members. However, in the preferred type, the bottom, side walls and capping members are in the form of an integral unit, and the intersections of the parts are preferably pure edges. In a highly preferred configuration, the bioreactor configuration can be specifically designed as an extended triangular structure, which can accommodate a large amount of liquid phase. The capping member is preferably slightly arched, so that the capping member has a generally convex upper surface. In this type, it is better to have an exit and it is convex toward the middle of the upper surface, so -10- This paper size applies the Chinese National Standard (CNS) A4 specification (210X 297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs B7 V. Description of the invention (8) The body can come out of the chamber. There is also a filter at the outlet, which should at least prevent particulate matter from entering the cell. Furthermore, the outlet can also be used as a sampling point ′ to sample the biomass from the bioreactor chamber. Although the outlet can be directly connected to the atmosphere, it can also be connected to a manifold and then to an oxygen separation and ventilation system. The purged gas containing unused carbon dioxide can be collected from the outlet and its oxygen content can be eliminated before returning to the bioreactor to help the retention and effective use of carbon dioxide. The car is good, and the biological reaction benefit also includes a temperature control component that can control the temperature of the liquid phase. In one type, the temperature control member also includes more than one heat exchange duct, which forms a fluid path or a path that can be used for circulating the heat exchange medium in a small room. In order to further regulate the circulation of the chamber contents, the circulation member may further include a blocking plate. The blocking plate is positioned in the chamber and positioned in a manner to further enhance the circulation of the contents of the chamber. In a particularly preferred type, the temperature control member may be of a suitable type so that the resistance plate (if any) is integrally formed with the temperature control member, or the temperature control member may have a function of regulating the temperature in the small A and providing the resistance plate member. Dual role. The bioreactor may also include a pH control member that controls the pH of the liquid phase. The pH control means preferably comprises a pH probe in a liquid phase, the probe being operatively connected to a source of CO2. The pH of the liquid phase can be adjusted by controlling the delivery of CO2 or adding other chemicals. Under certain conditions, the light that diffuses naturally into a small room may not be optimal and it would be beneficial to enhance the distribution of light in the small room under these conditions. In this regard, the biological response is better located at the bottom of the light reflection, and the substrate can ensure (please read the precautions on the back before filling in this page)

-11 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Description of the invention (9) The light delivered from outside sources to the 'small room is the largest. In addition or in another version, the bioreactor may include more than one light-transmitting member, which promotes the effective distribution of light in the bioreactor. The light conducting member can be located inside or outside the bioreactor. If the light source is in the reactor, it can float on or be immersed in the liquid phase. If the light source is outside the bioreactor, it can be anywhere around it. Preferably, the light source is located below the side wall of the bioreactor. In a preferred aspect of the invention, the bioreactor can be suspended or positioned in a light box, where the angle of the light source is substantially perpendicular to the side wall of the container. In a preferred embodiment of the present invention, the bioreactor may include more than one type of jacket member, which may at least partially cover the outer surface of the bioreactor. When present, the jacketed component may provide an additional component to isolate the bioreactor. Although any component known in the art can be used to support a jacket layer surrounding the outer surface of the bioreactor, the jacket layer is preferably supported on the outer wall by a skeleton 戋 grid arrangement fixed to the cell. The skeleton or lattice can be made of any material: such as wood, metal or plastic, and preferably has a large caliber, which can be used in a bioreactor chamber. Although the jacket member can be formed of any material suitable for the compartment, the cooker is preferably made of a transparent material that allows all light to enter the bioreactor. It should be understood that the jacket member can provide a component for heating or cooling the bioreactor on the outside. In this example, the jacket member preferably extends to the skeleton or grid row, to the outer wall of the bioreactor, and to the bottom of the bioreactor. It is better to add… to the jacket member so that the heat exchange medium can be maintained. For example, the sheathing member may include a transparent flexible polyethylene or polyacetylene film, and the skeleton structure outside the reactor is closely connected. Hot or cold air recirculation = -12 sheet paper rule relies on financial records) A4 size (21〇χ297 公 楚 (Please read the precautions on the back before filling in this page)

5. Description of the invention (10) pieces and the appearance of the bioreactor. The pump component is preferably connected to a kind of internal temperature and regulation cycle of the bioreactor; = 2 traceable internal constant temperature. This control component can also be connected to the heat exchange 2 quasi-holding chamber. A = ::: sleeve: component can also be provided on the outer surface of the bioreactor. Recording adjusts the amount of light entering the chamber. For this purpose, although Λ ,, τ, any of the known strains in the art can be used, but the light control member is better: it is composed of-series curtains, which can be opened or closed to change into a chamber-like shape ί 量。 Amount. Preferably, the light control member is connected to more than one kind of light measuring member. When the amount of light measured by the component exceeds a preset reference value, the control member can turn off the light amount control member for a certain time, and then turn on the light control member. . i Bioreactors can also be fluidly connected to separate containers containing liquid phases. Separate containers can act as storage tanks, increasing the volume of the system. It can function. Such as dark phase area, in the high optical density and growth conditions. In addition, it can also function as a deposition system, whereby biomass is removed from the bioreactor. When used in this way, the bioreactor can be connected to existing technologies to control the flow rate from the liquid phase to the deposition vessel and enhance the deposition of biomass in the sedimentation vessel. Biomass can be removed continuously or intermittently, plus deposited material that has been collected and processed. This process can be used as a harvesting system to remove biomass from the system and maintain optimal cell density in the liquid phase. Bioreactors can also be used in a variety of locations, where they experience a variety of climatic conditions. The environment in the container and this mesozoic. The material experience is better to maintain a fixed for cell growth, survival, or specific biochemical processes in culture. -13 This paper size applies to Chinese National Standard (CNS) Α4 specifications (210 × 297 (Mm) # Jt Read the notes on this page and write this page. Printed by the Consumers 'Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. Printed by A7 B7. This can be achieved using a closed system bioreactor, where non-desired organisms can be eliminated and the physical environment of the liquid phase in the bioreactor can be controlled manually. The use of standard computer control and electronic sensors can achieve the control of physical and chemical conditions in the bioreactor. Chemicals used to maintain nutrition levels and control pH and other factors can be automatically added directly to the liquid phase in the bioreactor. Computer control can also control the temperature of the liquid phase in the container, which is to control the heat exchange system in the bioreactor or remove the culture in the main container and pass a simple heat exchanger in a temperature-controlled water bath. The removal and passage of the liquid phase containing biomass from the bioreactor to another container containing the gas delivery system and back to the bioreactor can be used to increase the dissolved carbon dioxide concentration in the liquid phase. In this version, the biomass can be removed via a taken-out bubble mixer, or other components that can dissolve carbon dioxide or other gases into the liquid phase without losing biomass in the environment or threatening the bioreactor. The removal and passage of the liquid phase can also be used to control the temperature of the liquid phase. In this type, before the liquid phase returns to the bioreactor, the temperature of the liquid phase can be changed by a container containing at least a temperature control member. The present invention also includes a single bioreactor tank, or both Many slots. If multi-tank type is provided, more than one small chamber can cover the stool care department to help the operation and control of dark phase growth and breathing. In this example, the liquid phase flow between [] and $ is preferably controlled by a computer component, which can track the cell density in each chamber, and can reflect the change in cell density between chambers, including night eyes (including Biomass). This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) ——.- I, ----_ 衣 丨 丨 (Please read the precautions on the back before filling this page) Order A7 B7 V. Description of the invention (12) It should be understood that the bioreactor described herein can be used on a batch basis, where a single culture of biomass can be grown in the bioreactor at any time 'or it can be used in a continuous production cycle The basic nutrients and liquid phase are stably filled into the bioreactor, and the liquid phase containing the cultivated biomass is continuously discharged out of the bioreactor. In a single batch operation, nutrients can be added to the bioreactor chamber, mixed with the biomass-containing solution, and set aside for a period of time until the required biomass density is reached. The resulting mixture is removed from the bioreactor for disposal. It should be noted that this type of operation can cause quite unstable environmental conditions in the bioreactor chamber, as nutrient concentrations and cell densities change over time. As for another different type, in the operation sequence which depends on the nature and intensity of light, the concentration of nutrient solution, and the biomass in the liquid phase, the basic nutrient is added to the liquid phase to maintain the growth rate of the biomass. The basic nutrients needed for better biomass growth and proliferation can be added to the bioreactor chamber when it is consumed by the biomass. In order to effectively track the change of this nutrient, it is better to have more than one component to be tested in the bioreactor chamber, which can detect the change of nutrient level. Better detection components can be connected to computer-controlled components, which can track nutrient levels in the liquid phase and introduce nutrients into the bioreactor chamber when needed. If a large volume of biomass is needed (such as the commercial production of biomass), the bioreactor chamber is preferably operated in a continuous production cycle, where new nutrients can be stably added to the chamber and the biomass can be stably discharged. If there are multiple organisms. Anti-15- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) ---- II.-Iφΐ- (Read the meaning of Vi on the back of the poem before filling this page) Order Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A 7 B7 V. Description of the invention (13) The reactor series is connected, and the first bioreactor can provide stable addition of new reactants. The continuous flow of reactant material passes from one bioreactor to the next. The cultivated biomass is continuously discharged from the last of the series of bioreactors. In some cases, additional nutrients or other compounds are introduced into each bioreactor in the series (ie, the bioreactor will empty this nutrient or other compound). Completely mixing the contents of the liquid phase in the bioreactor means that a fixed biomass concentration can be maintained in and out of each bioreactor. This can cause the biomass concentration to change from one bioreactor to the next. The concentration of better biomass will increase as it passes through a series of bioreactors. One of the advantages of using many bioreactors in a continuous series to produce biomass is that in addition to the simplicity of construction, it is easy to control temperature and pH 値. Materials entering specific containers can be immediately drilled into large-volume biomass, and local changes in temperature and pH 値 are minimized due to agitation. The present invention will be explained in more detail by examples with reference to the accompanying drawings, wherein: FIG. 1 is a perspective view from above the bioreactor in a preferred embodiment of the present invention; FIG. 2 is a longitudinal cross section of the bioreactor of FIG. 1; Printed by the Ministry of Standards and Staff ’s Consumer Cooperatives (please read the notes on the back before writing this page) Figure 3 is a simplified end view of the bioreactor in Figure 1, where the arrow indicates the liquid phase flow pattern in the small room; Figure 4 'It is a plot of the biomass versus time obtained from the average results of two different bioreactors of a similar type to the bioreactor of Fig. 1. The bioreactors each have a volume of 750 liters and a triangle size of 1200 mm is 1200 mm long. -This paper size applies to China National Standard (CNS) A4 (210X297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (14) Figure 5 is a similar version of the bioreactor shown in Figure 1. A plot of the time within the biomass obtained from the average results of different bioreactors. Each of the bioreactors has a volume of 450 liters, is 600 millimeters long, and has a size of 3000 millimeters. Figure 6 shows 1 similar to FIG bioreactor two different types of bioreactors is obtained from the biomass are plotted vs time, the bioreactor # 1 350-liter. Fig. 7 is a plot of biomass versus time obtained using a bioreactor similar to the bioreactor of Fig. 1. Each of the bioreactors has a volume of 3500 liters and has a triangle size of 900 mm and a length of 10 meters. The figure shows the curve of eight different processes. The first journey took place on September 29, 1995. The second journey took place on June 14, 1995. The third journey took place on June 29, 1995. The fourth course took place on July M, 1995. The fifth course took place on August 4, 1995. The sixth course was conducted on September 丨 1995. The 7th process is performed on ^ "year ^ month." The 8th process is performed on 1995.17. The bioreactor shown in Figs. 1 and 3 and generally numbered 10 includes three vertical supports 14 Supported cells 12, two of which are pressed at each end and the third is located in the middle of cell 12. Cell 12 is an inverted, generally triangular cross-section cell, and has a slightly convex cover member 126, The closing chamber 12 further includes a narrow base portion 18 and—opposite to the side wall 20 =. The small loop-shaped circulation member 22 is located in the chamber, 』facing the chamber 1? &Gt; I bottom 1S. Catheter 22 There are many perforations 24, along the dragon's earth gas in a substantially downward direction through the conduit g-, so that the perforations enter and exit again, so _-17- ΪThe paper size is suitable for Chinese national standards] CNS) E Grid (21〇χ297 Male Thin) ~~ · ——----- (Please read the notes on the back before filling this page)

A7 B7 V. Description of the invention (15) Avoid particulate matter from depositing under the conduit 22. The conduit 22 in the specific example extends through the chamber and partially projects from either end, allowing many bioreactors to be connected in series. _ The temperature-controlling member 26 in the form of a duct pair is positioned facing the middle of the cell 12 and along the length of the cell 12. The conduit 26 is connected to an external supply of heat exchange medium (not shown). Therefore, the temperature of the liquid phase in the small room can be adjusted to any predetermined point through the use of the thermostat and the temperature control of the heat exchange medium. The capping member 16 provides a pair of vent holes 28. The vent holes 28 are located at each end of the capping member 16 and face the center of the capping member 16, so that the highest portion of the convex surface of the capping member 16 can be adjusted. During operation, there is positive pressure in the vent hole 28, so that excessive gas can flow out of the vent hole, and no fluid flows into the chamber through the vent hole. Therefore, it is avoided to bring air-borne pollutants into the chamber through the vent hole 28. Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page). During operation, according to the bioreactor of Figures 1 to 3, the cell 12 has a unique liquid flow pattern. The flow pattern is particularly shown in FIG. 3. The air entering the chamber through the duct 22 directly flows down to the lower end 18 of the base portion, and then runs vertically through the chamber until it hits the bottom end of the capping member 16. Once in contact with the capping member, air is pushed toward the side wall 20. Excess air leaves the cell through the vent hole 28. The passage of air and its interaction with the liquid phase and its interaction with the inner wall of the cell 12 enable the liquid phase to circulate according to the arrows in FIG. 3. Changing the flow direction in the liquid phase, its effect allows the liquid phase containing biomass to circulate vertically upward through the middle area of the cell 12, pass through the upper end of the cell directly under the capping member 16, and flow into the duct 22 adjacent to the side wall 20 and downward , And then repeat the cycle. Furthermore, the travel of the liquid phase in the small chamber 16 can also maintain the side walls 20 and -18 of the small chamber 12- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) A7 B7 V. Description of the invention (16) Component 16 certification. Liquid phase flow and the inclined surface of the cell wall can prevent biomass from depositing on the wall, and linear movement of the liquid phase can reduce the tendency of certain types of biomass, such as algae, to adhere to the cell wall. The flow of the liquid phase and the configuration of the cell 12 also cause the capping member 16 of the cell 12 to come into contact with the liquid phase, which prevents condensation from forming on the capping member 16. When the biomass contains light self-supporting substances, the capping member 16 represents an advantageous photo-synthetic surface, and the condensation effect on the capping member 16 may cause loss of photosynthetic active irradiation. It will be seen that this circulation pattern will cause the biomass in the small room to be regularly positioned adjacent to the capping member 16 and the side wall 20, so that the biomass can also face the light introduced into the small room. It can be noted here that the cell density of the biomass in the chamber is quite low, and the light penetration into the chamber is still quite high. As the cell density of the biomass increases, the light penetrating into the cell can be significantly reduced. Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (#Read the notes on the back before filling this page) In order to achieve the highest luminous efficiency, the bioreactor can be oriented in the north-south direction, and the tolerance of the position is appropriately corrected. Follow the guidelines established by solar recycling. In this basic north-south position, the bioreactors can be placed in parallel and at a distance equal to twice the size of the triangle of the container. This arrangement contributes to the maximum efficiency of light obtained by the bioreactor and the use of land. This is based on the maximum efficiency of direct light for 8 hours, rather than 12 hours during the day. There is no shadow effect in the adjacent tank. Bioreactors can be designed to be cleaned and disinfected in situ. The structure of a better bioreactor reflects the steps of an open food industry where food-grade ingredients are used and also helps to effectively clean and sanitize the container and all associated underlying structures. -19- This paper size applies to Chinese National Standards (CNS) A4 specifications (210'X: 297 mm) A7 B7 V. Description of the invention (17) It is better that all things added to the small room are disinfected. The air was filtered through 0.2 micrometers and also treated with ultraviolet rays at the point where it was supplied to the bioreactor. The water and nutrient medium added to the chamber can be filtered through 0 micron, and can also be treated with UV irradiation. The inoculum in the container is preferably grown under hygienic conditions and added to the bioreactor under hygienic conditions. The culture density in the chamber can be manipulated to maintain a culture density that can be used for continuous or semi-continuous harvesting processes, or to facilitate batch harvesting systems. The choice of harvesting system and process depends at least on the nature of the production system, the biomass used and the end use of the harvested product. Triangular bioreactors have been tested that are substantially longer than the cross-sectional dimensions. Biomass growth rates have been found to be relatively unaffected by the length of the bioreactor. Conversely, the size of the triangle in the cross section seems to be an important predictor of growth rate. Therefore, the present invention can be enlarged to a maximum volume without significantly changing the growth rate of the biomass. Figure 4 shows the increase in biomass over time in a triangle-shaped bioreactor with a volume of 750 liters and the same side length of 1200 mm. The 75-litre bioreactor produces 1,000 milligrams of biomass per litre in 6 days under natural daylight conditions. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read and read the precautions of vg before filling out this page). Figure 5 shows the increase of biomass over time, which is at a volume of 450 liters and a length of 600 mm. Triangular cross section and 3 meters in length. The 45 liter bioreactor can generate 1,200 milligrams of biomass per liter under natural daylight conditions within 4 days. Fig. 6 shows that the biomass increases with time in a triangle cross section with a volume of 350 liters and a length of 900 mm and a 1-meter-long bioreactor. 35〇L-20- This paper size applies Chinese National Standard (CNS) A4 regulations; fgT ^ 70 × 297mm) V. Description of the invention (18) The bioreactor can generate per liter in about 6 days under natural daytime conditions About 1000-1200 mg of biomass. Figure 7 shows the increase in biomass over time, in a 3500-liter volume and a 9-meter-long measuring cross-section in a polygonal shape and a bioreactor with a length of 0 meters. The liter bioreactor can produce about 1 100 mg of biomass per liter under natural daytime conditions in about 14-15 days. The yield of this container averages 63 mg / L / day (more than 4 months) in winter and about 120 mg / L / day in summer. This is equivalent to 19.85 g / day or U.95 tons / ha for 4 months in winter, and 37.8 g / day or 45.53 tons / ha for 8 months in summer. These data are estimated from a 900-mm-diagonal bucket, which has a volume of 350 liters / meter and is arranged as in the previous container, the containers are arranged in parallel, and the distance from the base end to the base end is 180 mm. This arrangement results in a total theoretical volume of 1925 m3 per hectare. It should be understood that the present invention makes it automatically controllable, in particular, via a computer. Furthermore, the continuous connection of the bioreactors of the specific examples of Figures 1 to 3 will produce a continuous production cycle for the production of a large amount of biomass. It should be understood that there can still be further &amp; changes, and it is obvious to the artisan from the above indication, that such changes are determined to be within the scope of the invention disclosed herein. Printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs This paper is sized for China National Standard (CNS) A4 (210X; 297 mm)

Claims (1)

No. 85100275 Patent Application BS Earthwork Application Patent Model Amendment (89 vehicles February, D8 VI, Patent Application Model 5 years, amended in May. Α Supplement 1. A kind of bioreactor for biomass production. 〇). It includes: (i) a substantially transparent chamber (12), which is at least suitable for containing biomass in a liquid phase, and has a base portion (18), a top portion, and a large number of the two in between. The side wall (20), the side wall (20) is structured to be generally detachable from the base portion (18) to the top; and (Π) a circulation member (22) of the contents of the circulation chamber (12), wherein the circulation member (22) ) Can generate suitable moving force in the liquid phase to ensure that all biomass on the mass can be continuously mixed, and at least semi-continuously expose the biomass to the light source. 2. Bioreactor (丨 0) according to item 1 of the scope of patent application, wherein the circulation rate in the chamber (work 2) is at least slightly higher than the rate of biomass deposition in the bioreactor (丨 〇). 3. The bioreactor (10) according to item 1 of the patent application scope, wherein the bioreactor (10) is suspended above the surface of the reflective substrate. 4. The bioreactor (10) according to the scope of the patent application, wherein the circulation member (22) is located at or near the base portion (18) in the bioreactor (10). 5. The bioreactor (10) according to the scope of the patent application, wherein the circulating structure (22) is located near the base portion (18) in the bioreactor (10), and can generate biomass and liquid The upward movement of the phase from the base part (丨 8) to the top of the bioreactor (10) is caused by the biomass that can impact the upper surface of the container to ensure a short exposure of most of the biomass in the liquid phase, after which the biomass is outward Move to the side wall of the bioreactor and then down to the base part (18), so as to capture and drive the circulation component again to the top of the bioreactor (丨 0). 6. According to the bioreactor (1) of the scope of the patent application, the circulation structure ϋν tn I ^^^ 1 1 ^ 1 * m BB ^ i ^ nn i al m nn one * i (Please read first (Notes on the back, please fill out this page) Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs No. 85100275 Patent Application BS Earthwork Application Patent Model Amendment (89 vehicles February, D8 VI, Patent Application Model 5 years, amended in May. Α Supplement 1. A kind of bioreactor for biomass production. 〇). It includes: (i) a substantially transparent chamber (12), which is at least suitable for containing biomass in a liquid phase, and has a base portion (18), a top portion, and a large number of the two in between. The side wall (20), the side wall (20) is structured to be generally detachable from the base portion (18) to the top; and (Π) a circulation member (22) of the contents of the circulation chamber (12), wherein the circulation member (22) ) Can generate suitable moving force in the liquid phase to ensure that all biomass on the mass can be continuously mixed, and at least semi-continuously expose the biomass to the light source. 2. Bioreactor (丨 0) according to item 1 of the scope of patent application, wherein the circulation rate in the chamber (work 2) is at least slightly higher than the rate of biomass deposition in the bioreactor (丨 〇). 3. The bioreactor (10) according to item 1 of the patent application scope, wherein the bioreactor (10) is suspended above the surface of the reflective substrate. 4. The bioreactor (10) according to the scope of the patent application, wherein the circulation member (22) is located at or near the base portion (18) in the bioreactor (10). 5. The bioreactor (10) according to the scope of the patent application, wherein the circulating structure (22) is located near the base portion (18) in the bioreactor (10), and can generate biomass and liquid The upward movement of the phase from the base part (丨 8) to the top of the bioreactor (10) is caused by the biomass that can impact the upper surface of the container to ensure a short exposure of most of the biomass in the liquid phase, after which the biomass is outward Move to the side wall of the bioreactor and then down to the base part (18), so as to capture and drive the circulation component again to the top of the bioreactor (丨 0). 6. According to the bioreactor (1) of the scope of the patent application, the circulation structure ϋν tn I ^^^ 1 1 ^ 1 * m BB ^ i ^ nn i al m nn one * i (Please read first (Notes on the back, please fill out this page) Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A8 B8 C8 D8 Scope of patent application Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs ~ 2 pieces-a kind of catheter, which extends along the length of the chamber (12) and is located near the chamber. &Lt; Base material (18) 'The catheter is The perforated money is connected to the gas source, such as 2 when the gas can run out of the shirt and enter the Mi2), resulting in the circulation of the contents of the room. 7. The bioreactor 根据 according to item i of the patented patent park, which includes ⑴ a substantially transparent chamber (12), Xuan ⑽ is at least suitable for containing the main Xin phase "biomass, and has a narrow base portion ( 18), which can be the sealed top of the capping member (16) and the many side walls (20) separated from the base part to the top; and Gi)-a circulation member (22) that can recirculate the contents of the chamber (12), The circulation member (22) can generate a suitable moving force to ensure continuous mixing of the liquid phase and expose the biomass to light. The circulation member (22) is located at the bottom or near the base portion (18). It also includes a duct extending along the bioreactor ⑽). The duct is perforated and connected to the gas source. The perforation (24) of the catheter foot faces the bottom of the duct. In this way, the gas runs out of the duct during operation and then the liquid The phase is upward, to (a) avoid the deposition of indoor particulate matter under the duct, (b) cause the circulation of the liquid phase; and (c) diffuse the gas to the liquid phase. 8. The bioreactor (ι0) according to the item of the patent scope, wherein the bioreactor chamber (12) is an elongated trough-shaped prism, and the prism has an inverted triangular cross section, which can accommodate a large amount of liquid phase. 9. The bioreactor (10) according to item 7 of the scope of the patent application, wherein the cap member (16) is slightly arched, and usually has a convex upper surface, and more than one vent hole (28) faces The middle of the convex upper surface allows the gas to be opened in the chamber (12) from the applicable national standard of t (c is called A4 · (2 敝 297) (please read the precautions on the back before filling this page) A8, B8, C8, D8 are printed on the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs, and the scope of patent application is transparent. 10 · According to the bioreactor (9) of the scope of the patent application, the ventilation hole (28) includes a filtering member, which can prevent particulate matter from entering the room. Bioreactor (10) 'wherein the bioreactor (10) includes a temperature control member (26) that can control the temperature of the liquid phase. 12. According to the bioreactor (10) of the patent application park No. 1 or 7, Wherein the bioreactor (10) contains at least one baffle, and its location way can further enhance the circulation of the contents of the chamber (12). 13. According to the bioreactor (10) of the scope of application for patents 1 or 7, where the biological The reactor (10) includes a pH control member capable of controlling the pH of the liquid phase. 14. The bioreactor according to the scope of the patent application] or item 7, wherein the bioreactor (10) includes more than one light guide member, It can promote the effective distribution of light in the room (12). 15_ The bioreactor according to item i or 7 of the scope of the patent application, wherein the bioreactor (10) contains more than one detection component, which can detect nutrition Change of level 16. According to application The bioreactor (10) in the fifth range, wherein the detection component is connected to the computer control component, which can track the nutrition level in the liquid phase and can introduce nutrients into the bioreactor chamber when necessary (12 ). 17_ Bioreactor (10) according to the scope of the patent application] or item 7, wherein the bioreactor (10) includes more than one jacket member, which can at least partially cover the bioreactor (10 18) The bioreactor (10) according to item i or 7 of the scope of patent application, wherein the bioreactor (10) is connected to a separate container in a fluid, whereby the liquid phase is in the back (read (Read the note $ on the back before filling out this page) 6. Scope of patent application Escape before the bioreactor (10). (Please read the precautions on the back before filling in this page) 19. According to the bioreactor (10) of the item i 8 of the scope of patent application, the separate container is a storage tank, which can increase the volume of the system. 20. According to the bioreactor (10) in the scope of the patent application, the separate reactors each have a dark phase area, which is used when the light intensity is high and the growth conditions. 21. According to the scope of the patent application, The bioreactor (10) of item 8, wherein the separate container is a deposition system whereby the biomass can be removed from the liquid phase. 22. The bioreactor (10) according to item 1 or 7 of the scope of patent application, wherein the internal environment of the bioreactor (10) is kept fixed and optimal for biomass growth and survival or can induce specific within the biomass In the biochemical process, more than one computer component can be used at this point, which can introduce nutrients or chemicals into the light or temperature of the liquid phase to reflect changes in more than one condition. 23. The bioreactor (10) according to the scope of application patent No. 丨 or 7, wherein the liquid phase is pumped in through a separate container containing a bubble contactor that is taken away, or other carbon dioxide or other gas can be dissolved into the liquid phase The components are returned to the bioreactor (10). 24. According to the bioreactor (3) of the scope of application for patent No. 23, which is printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Liquid Economics and also via a container pump containing a temperature-controlling member (26) that can change the liquid temperature And then back to the bioreactor (10). 25. The bioreactor (10) according to the scope of the patent application No. 7 or 7, wherein the bioreactor (10) includes a plurality of chambers, all of which are connected to each other. 26. According to the bioreactor (1-5) of the scope of application for patent, one or more of the chambers (12) are covered to make the darkness to help the biomass multiply. This paper size is applicable to the National Standards of Ten Countries (CNS) Eighty-four Secrets (21〇χ2 ^ 7 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Public Economy A8 B8 C8 D8 Patent Application Scope 27. According to the 25 The reactor (⑼, the liquid phase flow between the towel chamber (η) is controlled by a computer control component, which can track the biomass density in each chamber (I), and can reflect the biomass in each chamber (12). Density changes to transfer liquid phase between chambers (12). 28. A method of cultivating biomass, the method comprising cultivating biomass in a bioreactor (10) according to item 1 of the patent application, wherein the organism It grows in a continuous production and production cycle, where the necessary nutrients and liquid phase are stably added to the Z-reactor (10), and the liquid phase containing the cultivated biomass is continuously discharged from the bioreactor (10). This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page)