TW202146340A - Processing method for wastewater containing tetramethylammonium chloride and processing device for wastewater containing tetramethylammonium chloride - Google Patents

Abstract

The present disclosure provides a processing method for wastewater containing tetramethylammonium chloride including the following steps. A microbial composition is provided, wherein the microbial composition includes a microorganism of the genusOchrobactrum sp. , a microorganism of the genusAchromobacter sp. and a microorganism of the genusMycobacterium sp . A mixing step is performed, wherein the microbial composition is added into a wastewater containing tetramethylammonium chloride and mixed evenly so as to obtain a mixture. A reaction step is performed, wherein the mixture is reacted for a reaction time so as to degrade a tetramethylammonium chloride of the wastewater containing tetramethylammonium chloride. Therefore, the processing method for wastewater containing tetramethylammonium chloride of the present disclosure can degrade the tetramethylammonium chloride of the wastewater containing tetramethylammonium chloride, so that the processing method for wastewater containing tetramethylammonium chloride of the present disclosure has application potential in the relevant market.

Description

Treatment method of wastewater containing tetramethyl ammonium chloride and wastewater treatment equipment containing tetramethyl ammonium chloride

The present invention relates to a wastewater treatment method, in particular to a wastewater treatment method containing tetramethylammonium chloride.

Tetramethylammonium chloride (TMAC) is a chemical reagent widely used in industrial preparation. In recent years, it is more commonly used as a developer, phase transfer catalyst, template, polarographic reagent or precious metal separation reagent for circuit boards. . When tetramethyl ammonium chloride is used to extract and separate the aqueous phase and the organic phase, the wastewater will contain high concentrations of tetramethyl ammonium chloride, organic matter and ammonia nitrogen components. If it is directly discharged into the water body, it will cause serious environmental problems. destroy. Furthermore, for the human body, tetramethylammonium chloride is not only irritating to the eyes and skin, but also can cause acute poisoning reaction and endanger human health and life if accidentally inhaled or swallowed.

Therefore, how to develop a method that can effectively remove tetramethylammonium chloride in industrial wastewater not only has relevant market application potential, but also is particularly important for sustainable environmental protection practices.

One aspect of the present invention is to provide a method for treating wastewater containing tetramethylammonium chloride, comprising the following steps. A microbial composition is provided, wherein the aforementioned microbial composition comprises a microorganism of the genus Ochrobactrum sp. , a microorganism of the genus Achromobacter sp. and a microorganism of the genus Mycobacterium sp . A mixing step is performed, which is to add the aforementioned microbial composition to a waste water containing tetramethyl ammonium chloride and mix thoroughly to obtain a mixture. A reaction step is performed, wherein the aforementioned mixture is reacted for a reaction time to degrade tetramethylammonium chloride in wastewater containing tetramethylammonium chloride, wherein an initial pH value of the aforementioned mixture is 1-7.

According to the aforementioned method for treating wastewater containing tetramethylammonium chloride, wherein the concentration based on the aforementioned microbial composition is 100 weight percent, the mixed concentration of one of the microorganisms belonging to the genus Paleobacter, the microorganisms belonging to the genus Achromobacter and the microorganisms belonging to the genus Mycobacterium can be 50 weight percent to 85 weight percent.

According to the aforementioned method for treating wastewater containing tetramethylammonium chloride, wherein the concentration of the aforementioned microorganism composition is 100% by weight, the concentration of the microorganism of the genus Paleobacter can be 20% by weight to 28% by weight, and the concentration of the microorganism of the genus Achromobacter is 100% by weight. The concentration may be 20 to 32 weight percent, and the concentration of the microorganism of the genus Mycobacterium may be 7 to 30 weight percent.

According to the above-mentioned treatment method of wastewater containing tetramethyl ammonium chloride, an initial concentration of tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride can be 0.1 mg/L to 200,000 mg/L.

According to the aforementioned method for treating wastewater containing tetramethyl ammonium chloride, wherein the volume percentage of the microbial composition can be 5% to 15% based on the volume percentage of the aforementioned mixture being 100%.

According to the above-mentioned treatment method of waste water containing tetramethyl ammonium chloride, the reaction temperature of the above-mentioned reaction step can be 20 ℃ to 40 ℃.

According to the aforementioned method for treating wastewater containing tetramethylammonium chloride, the aforementioned reaction time may be 5 days to 25 days.

According to the aforementioned method for treating wastewater containing tetramethylammonium chloride, the initial pH value of the aforementioned mixture can be 3 to 7.

Another aspect of the present invention is to provide a tetramethyl ammonium chloride-containing wastewater treatment equipment, which is applied in the aforementioned treatment method of tetramethyl ammonium chloride-containing wastewater, the tetramethyl ammonium chloride-containing wastewater The wastewater treatment equipment includes a biological reaction tank and a solid-liquid phase separation tank. The biological reaction tank includes a biological aerobic reaction tank and a biological facultative oxygen reaction tank. The biological aerobic reaction tank includes a waste water input port, an adjustment liquid input port and a primary waste water output port, wherein the aforementioned microbial composition is accommodated in the biological aerobic reaction tank, and the waste water input port is used for inputting the aforementioned tetramethylmethane-containing The base ammonium chloride wastewater is sent to the biological aerobic reaction tank, and the adjustment liquid input port is used to input a pH adjustment liquid. The biological aerobic reaction tank is connected to the primary waste water output port and includes a treated water output port, wherein the biological aerobic reaction tank is used to receive a primary wastewater containing tetramethylammonium chloride output from the biological aerobic reaction tank. The solid-liquid phase separation tank is connected to the above-mentioned treated water output port and includes a discharge port, wherein the solid-liquid-phase separation tank is used to receive a treated water output from the above-mentioned biological facultative oxygen reaction tank and separate a solid component of the above-mentioned treated water, And the treated water is output from the discharge port.

According to the aforementioned tetramethylammonium chloride-containing wastewater treatment equipment, it further comprises a storage tank for pH adjustment liquid. The pH adjustment liquid storage tank is connected to the aforementioned adjustment liquid input port, and the pH adjustment liquid storage tank is used for storing the aforementioned pH adjustment liquid.

According to the aforementioned tetramethylammonium chloride-containing wastewater treatment equipment, a temperature control device is further included. The temperature control device is connected to the aforementioned biological reaction tank, and the temperature control device is used to control a reaction temperature of the aforementioned biological reaction tank.

Thereby, the method for treating wastewater containing tetramethyl ammonium chloride and the wastewater treatment equipment containing tetramethyl ammonium chloride of the present invention add a microbial composition comprising microorganisms belonging to the genus Paleobacter, microorganisms belonging to the genus Achromobacter and microorganisms belonging to the genus Mycobacterium In wastewater containing tetramethyl ammonium chloride, four of the wastewater containing tetramethyl ammonium chloride are degraded by utilizing the tetramethyl ammonium chloride degrading activity of microorganisms belonging to the genus Paleobacteria, microorganisms of the genus Achromobacter and microorganisms of the genus Mycobacterium Methyl ammonium chloride, supplemented by appropriate initial pH value and reaction temperature, can effectively achieve the purpose of wastewater treatment, and has application potential in related markets and industrial applications.

The following will illustrate specific test examples of the present invention with reference to the drawings, so that those skilled in the art to which the present invention pertains can fully utilize and practice the present invention without excessive interpretation and experimentation. However, the reader should understand that these practical details should not be used to limit the present invention, that is, in some experimental examples of the present invention, these practical details are not necessary, but are used to illustrate how to implement the present invention materials and methods.

[The treatment method of wastewater containing tetramethylammonium chloride of the present invention]

Please refer to FIG. 1 , which is a flow chart showing the steps of a method 100 for treating wastewater containing tetramethylammonium chloride according to the present invention. The method 100 for treating wastewater containing tetramethylammonium chloride includes step 110 , step 120 and step 130 .

Step 110 is to provide a microbial composition, wherein the microbial composition comprises a microorganism of the genus Ochrobactrum sp. , a microorganism of the genus Achromobacter sp. and a microorganism of the genus Mycobacterium sp . In detail, the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter, and the microorganisms of the genus Mycobacterium are all aerobic microorganisms, wherein the microorganisms of the genus Mycobacterium are aerobic acid-resistant microorganisms, which can treat tetramethyl-containing microorganisms in an acidic environment. The tetramethylammonium chloride in the ammonium chloride wastewater undergoes dehydrogenation and demethylation reactions to generate NH ₄ ⁺ -N, while the microorganisms of the genus Paleobacter and Achromobacter can further convert NH ₄ ⁺ -N Converted to ammonia to completely remove tetramethylammonium chloride from wastewater containing tetramethylammonium chloride. Furthermore, the microorganisms of the genus Paleobacter, the microorganism of the genus Achromobacter and the microorganism of the genus Mycobacterium of the microbial composition of the present invention can coexist in pollutants or wastewater containing high concentrations of tetramethylammonium chloride without affecting the activities of each other , so that the method 100 for treating tetramethylammonium chloride-containing wastewater of the present invention can be used to treat high-concentration tetramethylammonium chloride-containing wastewater, and has relevant market application potential. Furthermore, based on the concentration of the microbial composition being 100 wt %, the mixed concentration of one of the microorganisms of the genus Paleobacter, the microorganism of the genus Achromobacter and the microorganism of the genus Mycobacterium may be 50 wt % to 85 wt %. In addition, based on the concentration of the microbial composition being 100 weight percent, the concentration of the microorganism of the genus Paleobacter may be 20 to 28 percent by weight, the concentration of the microorganism of the genus Achromobacter may be 20 to 32 percent by weight, and the concentration of the microorganism of the genus Mycobacterium may be 20 to 32 percent by weight. The concentration can be from 7 weight percent to 30 weight percent.

Step 120 is a mixing step, which is to add the microbial composition of the present invention to a waste water containing tetramethylammonium chloride and mix thoroughly to obtain a mixture. Wherein, an initial concentration of tetramethylammonium chloride in wastewater containing tetramethylammonium chloride can be 0.1 mg/L to 200000 mg/L, and the volume percentage based on the mixture is 100%, the microbial composition of the present invention The volume percentage can be 5% to 15%. Alternatively, one of the initial concentrations of tetramethylammonium chloride in the wastewater containing tetramethylammonium chloride may be 250 mg/L to 2000 mg/L.

Step 130 is a reaction step of reacting the mixture for a reaction time to degrade tetramethylammonium chloride in the wastewater containing tetramethylammonium chloride, wherein an initial pH value of the mixture is 1-7. Wherein, a reaction temperature of the aforementioned reaction step can be 20 ℃ to 40 ℃, and the aforementioned reaction time can be 5 days to 25 days, to improve the microbial composition of the present invention to containing tetramethyl ammonium chloride in waste water The removal efficiency of tetramethylammonium chloride. Alternatively, the initial pH of the mixture may be from 3 to 7.

Thereby, the method 100 for treating wastewater containing tetramethyl ammonium chloride of the present invention permeates the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium to proliferate in the wastewater containing tetramethyl ammonium chloride, It degrades tetramethylammonium chloride in wastewater containing tetramethylammonium chloride through its growth and metabolism, thereby effectively achieving the purpose of wastewater treatment.

[The tetramethylammonium chloride-containing wastewater treatment equipment of the present invention]

Please refer to FIG. 2 , which is a schematic structural diagram of the tetramethylammonium chloride-containing wastewater treatment equipment 200 of the present invention. The tetramethylammonium chloride-containing wastewater treatment equipment 200 includes a biological reaction tank 210 and a solid-liquid phase separation tank 240 .

The biological reaction tank 210 includes a biological aerobic reaction tank 220 and a biological aerobic reaction tank 230 . The biological aerobic reaction tank 220 includes a waste water input port 221, an adjustment liquid input port 222 and a primary waste water output port 223, wherein the microbial composition of the present invention is accommodated in the biological aerobic reaction tank 220, and the wastewater input port 221 is used for The wastewater containing tetramethylammonium chloride is input into the biological aerobic reaction tank 220, and the adjustment liquid input port 222 is used for inputting a pH adjustment liquid. The biological aerobic reaction tank 230 is connected to the primary waste water output port 223 and includes a treated water output port 231 , wherein the biological aerobic reaction tank 230 is used for receiving a primary wastewater containing tetramethylammonium chloride output from the biological aerobic reaction tank 220 .

The solid-liquid phase separation tank 240 is connected to the treated water output port 231 and includes a discharge port 241, wherein the solid-liquid phase separation tank 240 is used to receive a treated water output from the biological facultative oxygen reaction tank 230 and separate a solid component of the treated water, and The treated water is output from the discharge port 241 .

Preferably, as shown in FIG. 2 , the tetramethylammonium chloride-containing wastewater treatment equipment 200 of the present invention may further include a pH adjusting solution storage tank 250 and a temperature control device (not shown). The pH adjustment solution storage tank 250 is connected to the adjustment solution input port 222, and the pH adjustment solution storage tank 250 is used for storing the pH adjustment solution, and the temperature control device is connected to the biological reaction tank 210, and the temperature control device is used for A reaction temperature of the biological reaction tank 210 is controlled.

In detail, when using the tetramethylammonium chloride-containing wastewater treatment equipment 200 of the present invention to process the tetramethylammonium chloride-containing wastewater, the tetramethylammonium chloride-containing wastewater will be input from the wastewater input port 221 to the wastewater containing tetramethylammonium chloride. The biological aerobic reaction tank 220 of the microbial composition of the invention, then, the pH adjustment liquid in the pH adjustment liquid storage tank 250 will be further input into the biological aerobic reaction tank 220 from the adjustment liquid input port 222 to adjust The initial pH value of the wastewater containing tetramethylammonium chloride is 1 to 7. Alternatively, the pH of the wastewater containing tetramethylammonium chloride can be adjusted to 3 to 7. Specifically, the pH adjustment solution can be H ₃ PO ₄ or NaOH solution. At the same time, the temperature control device will further adjust the reaction temperature of the biological reaction tank 210 to be 20°C to 40°C suitable for the growth of the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium, so as to facilitate the microorganisms of the genus Paleobacter , The proliferation of Achromobacter and Mycobacterium microorganisms and their activity of degrading tetramethylammonium chloride. Next, the tetramethylammonium chloride-containing primary waste water (including the microbial composition of the present invention and the tetramethylammonium chloride-containing waste water) that has completed the preliminary reaction in the biological aerobic reaction tank 220 will be input to the biological through the primary waste water output port 223 In the facultative reaction tank 230, the reaction is continued for a reaction time, so as to obtain a treated water, wherein the concentration of tetramethylammonium chloride in the above-mentioned treated water is very low or does not contain tetramethylammonium chloride. Finally, the aforementioned treated water will be further input into the solid-liquid phase separation tank 240 through the treated water output port 231 to further separate a solid component of the treated water, and the treated water will be output from the discharge port 241 . In detail, the solid content of the treated water can be the suspended matter of microorganisms after the reaction of the microorganism composition of the present invention with wastewater containing tetramethyl ammonium chloride or the solid impurities of wastewater containing tetramethyl ammonium chloride. After the static precipitation treatment, the treated water completed by the tetramethylammonium chloride-containing wastewater treatment equipment 200 of the present invention has met the national standard for discharge water (>10 mg/L) and can be discharged into the water body. The tetramethylammonium chloride-containing wastewater treatment equipment 200 of the present invention can effectively decompose the tetramethylammonium chloride in the tetramethylammonium chloride-containing wastewater, and has relevant market and industrial application potential.

[Examples and Comparative Examples]

Specific embodiments of the present invention will be proposed below to describe in detail the treatment method of the tetramethylammonium chloride-containing wastewater and the tetramethylammonium chloride-containing wastewater treatment equipment of the present invention for the treatment of tetramethylammonium chloride in the tetramethylammonium chloride-containing wastewater. The treatment capacity of tetramethyl ammonium chloride, wherein the treatment method of tetramethyl ammonium chloride-containing wastewater of the present invention will be operated with the tetramethyl ammonium chloride-containing wastewater treatment equipment of the present invention, and the relevant experimental operation flow and details please Refer to the previous paragraph, which will not be repeated here.

1. Tolerance test of microbial composition of the present invention to tetramethylammonium chloride

The tolerance test of the microorganism composition of the present invention to tetramethylammonium chloride is to cultivate the microorganism composition of the present invention in wastewater containing tetramethylammonium chloride, so as to observe the paleness of the microorganism composition of the present invention. Growth of microorganisms belonging to the genus Bacillus, Achromobacter and Mycobacterium.

In terms of experiments, firstly, the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium were cultivated in a fine-tuned M9 medium and acclimated to OD ₆₀₀ = 1 to obtain the microbial composition of the present invention, and then 10 The microbial composition of mL was added to the waste water containing tetramethyl ammonium chloride and was quantified to 100 mL with sterile water, and the concentration of tetramethyl ammonium chloride in the waste water was adjusted to 1000 mg/L. After culturing for 30 days under the conditions, the mixed growth of the microorganisms of the genus Paleobacter, the microorganism of the genus Achromobacter and the microorganism of the genus Mycobacterium in the microbial composition was observed, and the formula of the M9 medium is shown in Table 1. Table I M9 salt solution (10X) 100ml 20% glucose 20ml 1M MgSO ₄ 1 ml 1M CaCl ₂ 0.3 ml Biotin (1 mg/ml) 1 ml Thiamin 1 ml Trace elements solution (100X) 10 ml

In addition, in this experiment, samples of the mixture of microbial compositions and wastewater containing tetramethyl ammonium chloride were further taken for different incubation times for microbial overall genome sequencing (also known as 16s rRNA sequencing) to analyze the microbial composition of the present invention. The mixed concentration of Paleobacter, Achromobacter and Mycobacterium in wastewater containing tetramethylammonium chloride, and the individual concentrations of Paleobacter, Achromobacter and Mycobacterium concentration.

Please refer to FIG. 3 , which is a graph showing a mixed growth curve of the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium in the microbial composition of the present invention. As shown in Figure 3, under the condition that the concentration of tetramethylammonium chloride is 1000 mg/L, the microorganism composition of the present invention grows rapidly after culturing for 1 day, and its OD ₆₀₀ can reach more than 0.8. And after culturing for 30 days, the OD ₆₀₀ of the microbial composition of the present invention remains above 0.8, indicating that the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium in the microbial composition of the present invention can be contained in the microbial composition of the present invention. Proliferate and survive in ammonium chloride wastewater. Furthermore, the results of the sequencing of the overall genome of the microorganisms show that the concentration of the microorganism composition based on the present invention is 100 weight percent, and the mixed concentration of one of the microorganisms of the genus Paleobacter, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium is about 50%. wt% to 85 wt%, and the concentration of Paleobacter genus microorganisms is 20 wt% to 28 wt%, the concentration of Achromobacter genus microorganisms is 20 wt% to 32 wt%, and the concentration of Mycobacterium genus microorganisms is 7 wt% percent to 30 percent by weight.

The above results show that the microorganism of the genus Paleobacter, the microorganism of the genus Achromobacter and the microorganism of the genus Mycobacterium can coexist in the high-concentration wastewater containing tetramethylammonium chloride, and can maintain excellent growth. The activity means that the microbial composition of the present invention can be applied to degrade tetramethylammonium chloride in industrial wastewater or industrial waste, so that it has application potential in the relevant market and industrial utilization.

Please refer to Table 2, which series shows the experimental conditions of each embodiment of the present invention, and the following will continue to use the examples listed in Table 2 to illustrate the treatment method of the tetramethylammonium chloride-containing wastewater of the present invention at the start of different mixtures. Concentration changes of tetramethyl ammonium chloride at initial pH value, different initial concentrations of wastewater containing tetramethyl ammonium chloride and different reaction temperatures. Table II starting pH Reaction temperature (°C) Microbial composition (100%) Initial concentration of tetramethylammonium chloride (mg/L) Microbial concentration of Paleobacter (%) Achromobacter microbial concentration (%) Mycobacterium microbial concentration (%) Example 1 3 37 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 2 5 37 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 3 6.47 37 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 4 7 37 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 5 6.5 37 21.2~26.7 22.6~30.4 8.9~28.4 250 Example 6 6.5 37 21.2~26.7 22.6~30.4 8.9~28.4 500 Example 7 6.5 37 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 8 6.5 37 21.2~26.7 22.6~30.4 8.9~28.4 2000 Example 9 7.1 20 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 10 7.1 30 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 11 7.1 37 21.2~26.7 22.6~30.4 8.9~28.4 1000 Example 12 7.1 40 21.2~26.7 22.6~30.4 8.9~28.4 1000

The aforementioned examples 1-12 were all reacted and treated for 25 days, and samples of the mixture of the microbial composition and the wastewater containing tetramethylammonium chloride were taken for 0, 1, 2, 5, 10, and 25 days of reaction and analyzed. The concentration of tetramethyl ammonium chloride and NH ₄ ⁺ -N concentration, and further calculate the removal rate of tetramethyl ammonium chloride and the removal rate of NH ₄ ⁺ -N, where the concentration of tetramethyl ammonium chloride is The ion chromatograph analyzes the concentration of anions and cations present in a sample of a mixture of microbial compositions and wastewater containing tetramethyl ammonium chloride, and then converts the obtained values to the concentration of tetramethyl ammonium chloride, while tetramethyl ammonium chloride The removal rate of ammonium chloride is calculated by the calculation formula I of the removal rate of tetramethylammonium chloride, and the removal rate of NH ₄ ⁺ -N is calculated by the calculation formula II of the removal rate of _{NH 4} ^{+ -N} . The calculation formula I of the removal rate of tetramethylammonium chloride is as follows: Tetramethylammonium chloride removal rate (%) = ( C _{TMAC in} – C _{TMAC out} ) × 100 % (Formula I). C _{TMAC in} where C _{TMAC in} represents the initial concentration of _{tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride, and C TMAC out} represents the concentration of tetramethyl ammonium chloride after reaction in wastewater containing tetramethyl ammonium chloride . The calculation formula II of the NH ₄ ⁺ -N removal rate is as follows: NH ₄ ⁺ -N removal rate (%) = ( C(NH ₄ ⁺ T) – C(NH ₄ ⁺ A) ) × 100 % (Formula II). C(NH ₄ ⁺ T) Wherein C (NH ₄ ⁺ T) representative of wastewater theoretical concentration of NH ₄ ⁺ -N (i.e., a mole of tetramethylammonium chloride to produce a mole of NH ₄ ⁺ -N), and C (NH ₄ ⁺ A) represents the actual concentration _{of NH 4} ^{+ -N in the wastewater.}

2. The impact assessment of the initial pH value of different mixtures on the removal rate of tetramethylammonium chloride in the method for treating wastewater containing tetramethylammonium chloride of the present invention

This test is based on Example 1 to Example 4 to evaluate the effect of the microbial composition of the present invention and the wastewater containing tetramethyl ammonium chloride on the tetramethyl ammonium chloride-containing wastewater of the present invention at different initial pH values. Influence of tetramethylammonium chloride removal rate of ammonium wastewater treatment method. Please refer to FIG. 4, which shows the tetramethylammonium chloride in the tetramethylammonium chloride-containing wastewater at different initial pH values of the mixture according to the treatment method of the tetramethylammonium chloride-containing wastewater of the present invention. Analysis results of concentration change and removal rate of tetramethylammonium chloride.

As shown in Figure 4, after the second day of the reaction, the removal rates of tetramethylammonium chloride in Examples 1 to 4 all increased steadily with the increase of the reaction time, and after the 25th day of the reaction, The removal rate of tetramethylammonium chloride in Examples 1 to 4 can all reach more than 95%, wherein the molar fraction _{of NH 4} ^{+ -N in Examples 3 and 4 after the 25th day of reaction are both} Below 0.1, the _{removal rate of NH 4} ⁺ -N is 100% of Example 3 and 99.82% of Example 4, respectively, which shows that the treatment method of wastewater containing tetramethylammonium chloride of the present invention is effective in microbial composition and containing wastewater. Under acidic conditions where the initial pH of the mixture of tetramethylammonium chloride wastewater is 1 to 7, the microorganisms of the genus Paleobacteria, the microorganisms of the genus Achromobacter and the microorganisms of the genus Mycobacterium can effectively inhibit the tetramethyl chloride ammonium chloride undergoes dehydrogenation reaction and demethylation reaction to generate NH ₄ ⁺ _{-N, which can further decompose NH 4} ⁺ -N in the wastewater containing tetramethyl ammonium chloride, and then make the tetramethyl ammonium chloride-containing wastewater of the present invention. The treatment method of ammonium wastewater and the wastewater treatment equipment containing tetramethyl ammonium chloride can effectively decompose tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride, and have application potential in related markets and industrial applications.

3. Influence assessment of tetramethylammonium chloride-containing wastewater with different initial concentrations on the removal rate of tetramethylammonium chloride in the method for treating tetramethylammonium chloride-containing wastewater of the present invention

This test is based on Example 5 to Example 8 to evaluate the tetramethyl ammonium chloride containing wastewater with different initial concentrations for the treatment method of tetramethyl ammonium chloride wastewater of the present invention. Effect of ammonium removal rate. Please refer to FIG. 5, which shows the concentration change and the tetramethylammonium chloride concentration of the tetramethylammonium chloride-containing wastewater in the present invention for removing tetramethylammonium chloride-containing wastewater with different concentrations. The analysis results of the removal rate of tetramethylammonium chloride.

As shown in Figure 5, after the first day of the reaction, the removal rates of tetramethylammonium chloride in Examples 5 to 7 all increased with the increase of the reaction time, and after the 25th day of the reaction, the removal rate of tetramethylammonium chloride increased with the increase of the reaction time. The removal rate of tetramethylammonium chloride in Examples 5 to 7 can reach 100%, and _{the molar ratio of NH 4} ⁺ -N in Examples 5 to 7 after the 25th day of reaction are all lower than _{0.1, and the NH 4} ⁺ -N removal rate of Example 5, Example 6 and Example 7 can all reach 100%, and Example 8 is in tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride In the case of the initial concentration of 2000 mg/L, the removal rate of tetramethylammonium chloride is also higher than 50% after 25 days of reaction, showing that the microorganisms of the microbial composition of the present invention are of the genus Paleobacter and Achromobacter. and Mycobacterium microorganisms can also exert their activity of degrading tetramethylammonium chloride in high-concentration wastewater containing tetramethylammonium chloride, so the method for treating wastewater containing tetramethylammonium chloride of the present invention can Effectively remove tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride, so that the method for treating wastewater containing tetramethyl ammonium chloride and the wastewater treatment equipment containing tetramethyl ammonium chloride of the present invention have potential use In order to treat high-concentration wastewater containing tetramethyl ammonium chloride, it has application potential in related markets and industrial applications.

4. The influence assessment of different reaction temperatures on the removal rate of tetramethylammonium chloride in the method for treating wastewater containing tetramethylammonium chloride of the present invention

This experiment is based on Example 9 to Example 12 to evaluate the effect of different reaction temperatures on the removal rate of tetramethylammonium chloride in the method for treating wastewater containing tetramethylammonium chloride of the present invention. Please refer to FIG. 6, which shows the concentration change of tetramethylammonium chloride in the tetramethylammonium chloride-containing wastewater and the Analysis results of methyl ammonium chloride removal rate.

As shown in Figure 6, after the second day of the reaction, the removal rates of tetramethylammonium chloride in Examples 9 to 12 all increased with the increase of the reaction time, and after the 25th day of the reaction, the removal rate of tetramethylammonium chloride increased with the increase of the reaction time. The removal rate of tetramethylammonium chloride in Example 11 and Example 12 can reach 100%, the _{removal rate of NH 4} ⁺ -N can also reach more than 98.3%, and the molar ratio of NH ₄ ⁺ -N is less than 0.1. The ear fraction shows that under the condition that the reaction temperature of the method for treating wastewater containing tetramethyl ammonium chloride of the present invention is 20° C. to 40° C., the microorganisms of the genus Paleobacteria, the microorganisms of the genus Achromobacter and the Mycobacterium of the microbial composition are Microorganisms can effectively dehydrogenate and demethylate tetramethylammonium chloride to generate NH ₄ ⁺ -N, and can further decompose NH ₄ ⁺ -N in wastewater containing tetramethyl ammonium chloride, and then The method for treating wastewater containing tetramethyl ammonium chloride and the wastewater treatment equipment containing tetramethyl ammonium chloride of the present invention can effectively decompose tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride, and have a relevant market. and application potential for industrial use.

To sum up, the method for treating wastewater containing tetramethyl ammonium chloride and the wastewater treatment equipment containing tetramethyl ammonium chloride of the present invention add microorganisms comprising microorganisms belonging to the genus Paleobacter, microorganisms belonging to the genus Achromobacter and microorganisms belonging to the genus Mycobacterium The composition is used in wastewater containing tetramethyl ammonium chloride to degrade the tetramethyl ammonium chloride in wastewater containing tetramethyl ammonium chloride by utilizing the tetramethyl ammonium chloride degrading activity of microorganisms of the genus Paleobacter, microorganisms of the genus Achromobacter and microorganisms of the genus Mycobacterium. Tetramethylammonium chloride, supplemented by appropriate initial pH value and reaction temperature, can effectively achieve the purpose of wastewater treatment, and has application potential in related markets and industrial applications.

However, the present invention has been disclosed as above in an embodiment, but it is not intended to limit the present invention. Anyone who is familiar with the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection of the present invention The scope shall be determined by the scope of the appended patent application.

100: The treatment method of wastewater containing tetramethyl ammonium chloride 110, 120, 130: Steps 200: Wastewater treatment equipment containing tetramethylammonium chloride 210: Bioreactor 220: Biological aerobic reaction tank 221: Wastewater input port 222: Adjustment fluid input port 223: Primary waste water outlet 230: Biological facultative oxygen reaction tank 231: Treated water outlet 240: solid-liquid phase separation tank 241: Discharge port 250: pH adjustment liquid storage tank

In order to make the above and other objects, features, advantages and embodiments of the present invention more clearly understood, the accompanying drawings are described as follows: Fig. 1 is a flow chart showing the steps of the method for treating wastewater containing tetramethylammonium chloride of the present invention; Fig. 2 is a schematic diagram showing the structure of the tetramethylammonium chloride-containing wastewater treatment equipment of the present invention; Fig. 3 is a graph showing a mixed growth curve of the microorganism of the genus Paleobacter, the microorganism of the genus Achromobacter and the microorganism of the genus Mycobacterium in the microbial composition of the present invention; Fig. 4 shows the concentration change of tetramethyl ammonium chloride in the wastewater containing tetramethyl ammonium chloride and the tetramethyl ammonium chloride concentration in the wastewater containing tetramethyl ammonium chloride when the initial pH value of the mixture is different. Analysis result diagram of methyl ammonium chloride removal rate; Fig. 5 shows the concentration change of tetramethyl ammonium chloride and tetramethyl ammonium chloride in the treatment method of tetramethyl ammonium chloride-containing wastewater of the present invention for removing tetramethyl ammonium chloride-containing wastewater with different concentrations A graph of the analytical results of the removal rate of ammonium chloride; and Fig. 6 shows the concentration change of tetramethylammonium chloride in the wastewater containing tetramethylammonium chloride and tetramethylammonium chloride at different reaction temperatures of the method for treating wastewater containing tetramethylammonium chloride of the present invention Graph of analytical results of ammonium removal rate.

100: The treatment method of wastewater containing tetramethyl ammonium chloride

110, 120, 130: Steps

Claims (11)

A method for treating wastewater containing tetramethyl ammonium chloride, comprising the steps of: providing a microbial composition, wherein the microbial composition comprises a genus Ochrobactrum sp. microorganism, a genus Achromobacter sp. Microorganism and a Mycobacterium sp. microorganism; performing a mixing step of adding the microbial composition to a wastewater containing tetramethylammonium chloride and mixing thoroughly to obtain a mixture; and performing a reaction step, which is to react the mixture for a reaction time to degrade the tetramethylammonium chloride in the waste water containing tetramethylammonium chloride, wherein an initial pH value of the mixture is 1 to 7. The method for treating tetramethylammonium chloride-containing wastewater according to claim 1, wherein the microorganism of the genus Paleobacter, the microorganism of the genus Achromobacter and the microorganism of the genus Mycobacterium are based on the concentration of the microbial composition being 100 weight percent. One of the mixing concentrations is 50 to 85 weight percent. The method for treating wastewater containing tetramethylammonium chloride as claimed in claim 2, wherein based on the concentration of the microbial composition being 100 weight percent, the concentration of the Paleobacter microorganism is 20 weight percent to 28 weight percent, and the colorless The concentration of the microorganism of the genus Bacillus is 20 to 32 percent by weight, and the concentration of the microorganism of the genus Mycobacterium is 7 to 30 percent by weight. The method for treating tetramethylammonium chloride-containing wastewater according to claim 1, wherein an initial concentration of the tetramethylammonium chloride in the tetramethylammonium chloride-containing wastewater is 0.1 mg/L to 200,000 mg /L. The method for treating tetramethylammonium chloride-containing wastewater according to claim 2, wherein the volume percentage of the microbial composition is 5% to 15% based on 100% by volume of the mixture. The method for treating waste water containing tetramethyl ammonium chloride as claimed in claim 1, wherein a reaction temperature of this reaction step is 20°C to 40°C. The method for treating wastewater containing tetramethylammonium chloride as claimed in claim 1, wherein the reaction time is 5 to 25 days. The method for treating tetramethylammonium chloride-containing wastewater according to claim 1, wherein the initial pH value of the mixture is 3 to 7. A tetramethyl ammonium chloride-containing wastewater treatment equipment, which is applied in the treatment method of tetramethyl ammonium chloride-containing wastewater described in any one of claim 1 to claim 8, the tetramethyl ammonium chloride-containing wastewater Wastewater treatment equipment includes: A biological reaction tank, containing: A biological aerobic reaction tank, comprising a waste water input port, an adjustment liquid input port and a primary waste water output port, wherein the microbial composition is accommodated in the biological aerobic reaction tank, and the waste water input port is used for inputting the The tetramethylammonium chloride waste water is put into the biological aerobic reaction tank, and the adjustment liquid input port is used to input a pH adjustment liquid; and a biological aerobic reaction tank, communicated with the primary waste water output port and comprising a treated water output port, wherein the biological aerobic reaction tank is used to receive a primary wastewater containing tetramethyl ammonium chloride output from the biological aerobic reaction tank; as well as a solid-liquid phase separation tank, connected to the treated water output port and comprising a discharge port, wherein the solid-liquid phase separation tank is used to receive a treated water output from the biological facultative oxygen reaction tank and separate a solid component of the treated water, And the treated water is output from the discharge port. The tetramethylammonium chloride-containing wastewater treatment equipment as described in claim 9, further comprising: A pH adjustment liquid storage tank is connected to the adjustment liquid input port, and the pH adjustment liquid storage tank is used for storing the pH adjustment liquid. The tetramethylammonium chloride-containing wastewater treatment equipment as described in claim 9, further comprising: A temperature control device is connected to the biological reaction tank, and the temperature control device is used for controlling a reaction temperature of the biological reaction tank.

Images