TW201836694A - Blood filter device capable of separating blood cell and platelet rich plasma and achieving mass production - Google Patents

Abstract

A blood filter device of this invention comprises: a hollow sleeve provided above with an injection tube and a connection tube and below with a first discharge tube and a second discharge tube; a hollow filter tube located in an interior of the hollow sleeve and having an upper end mounted to a lower end of the injection tube of the hollow sleeve and a lower end mounted to an upper end of the second discharge tube of the hollow sleeve, wherein the hollow filter tube has a plurality of apertures; and an air extraction device connected to the connection tube of the hollow sleeve, wherein the air extraction device is used to make a pressure outside the hollow filter tube smaller than a pressure inside the hollow filter tube. When the blood filter device is filtering blood, a first valve of the connection tube, a second valve of the first discharge tube, and a third valve of the second discharge tube are in a closed condition.

Description

 Blood filter device  

The invention relates to a blood filtering device, in particular to a blood filtering device capable of separating blood cells from blood cells and platelets.

Platelet Rich Plasma (PRP) is a platelet concentrate isolated from blood. Platelet thick plasma contains a variety of growth factors. These growth factors can provide autologous repair materials, allow tissue regeneration, and terminate chondrocytes. Necrotic wear and tear, prevent inflammation and slow down pain, so platelet thick plasma is used in clinical research, testing, surgery, medical cosmetic, orthopedic surgery, hair regeneration, sports injury treatment, accelerated healing of burn wounds, etc., if you can go deep Discussion, I believe will definitely bring great contribution to human health.

At present, the more common method of separating platelet rich plasma (PRP) is to use the centrifugal technique to pass the whole blood blood into the test tube through the characteristics of large blood cell specific gravity, and to stratify the blood by centrifugal force generated by high-speed rotation. The upper layer of platelet thick plasma (PRP) is then extracted. See the following patents or products for obtaining platelet-rich plasma by centrifugation: Taiwan Patent No. I531401 utilizes one of the upper and lower bodies of the corresponding joint, the top of which is closed and has a predetermined number of convection holes. The lower part of the upper bottle body is provided with a separating plate. By rotating the upper bottle body and the lower bottle body, the insulating plate is driven to change the relative relationship with the convection hole to determine whether the upper bottle body and the lower bottle body are conductive or not. a suspension plug and a screw cap are arranged on the lower part of the lower bottle body, and the height and the low level of the suspension plug are changed by the adjustment of the screw cap, and the sleeve is set at the top of the upper bottle body, and the sleeve is inside the sleeve. A pipe column and a one-way valve are arranged, and a rotating seat and a regulating valve are arranged on the top of the sleeve, and are sealed by an upper cover, and two independent nozzles are arranged on the upper cover, and the rotating seat is provided And the regulation of the regulating valve determines whether the upper two columns are turned on or not, and another cap is provided to cover the two nozzles at the same time, thereby forming the closed platelet concentrator of the present invention, so that the centrifuge is used to perform the second Secondary centrifugation, blood Separated into a plasma layer was concentrated, platelet rich plasma, blood three different compositions.

Mainland China Patent Nos. CN202478263U and CN201469752U, both of which propose a conventional syringe as a blood drawing device. After taking blood, the injection needle is removed, and a container with a piston is attached to the opening of the syringe ( That is, the original injection needle socket), and then the assembled syringe is centrifuged together with the container sleeved thereon, and the syringe is placed on the top and the container is placed under the centrifuge; therefore, the plasma collection method after centrifugation is The blood layer at the bottom is drained downward into the container, and the thick plasma of the platelets is lowered to the opening of the syringe, and then the injection needle is placed to directly inject the platelet-rich plasma back into the blood-derived patient.

US Patent Application Publication No. 20100025342A1 proposes a blood separation device which is also based on the principle of a conventional syringe, which is a two-syringe, one of which is a first syringe for mounting an injection needle and extracting whole blood. After obtaining whole blood, the injection needle is replaced with a cap to seal the opening, and then a weaker centrifugation procedure is applied to initially separate the blood with the injection opening facing downward, and thereafter, a second syringe with an injection needle is used. Inserting the reserved tube of the first syringe into the reserved tube, sucking most of the initially separated plasma (ie, preliminary centrifugation to obtain the supernatant); then replacing the injection needle with another cap to seal the opening of the second syringe, and then sealing Strong centrifugation is performed to more accurately stratify the plasma and to vent a specific blood layer down (ie, in the direction of its gravity) to obtain platelet-rich plasma.

Products that obtain platelet-rich plasma by centrifugation:

GLO PRP kit:

Product Action Video URL

Https://www.youtube.com/watch? v=XJrOrCm8h2w

T-LAB PRP Kit:

Product Action Video URL

Https://www.youtube.com/watch? v=C68Mcug01X8

Proteal PRP Kit:

Product Action Video URL

Https://www.youtube.com/watch? v=lQV2h4OWOwo

Dr.PRP Kit

Product Action Video URL

Https://www.youtube.com/watch? v=e1IgqdeBtYo

GENESIS PRP

Product Action Video URL

Https://www.youtube.com/watch? v=494YeLv6mZE

However, the above patents or products for obtaining platelet thick plasma (PRP) by centrifugation have the following disadvantages.

1. The operation procedure is complicated. At present, the method used to obtain thick plasma of platelets is still mainly based on centrifugal tube extraction. After centrifugation of blood, in order to extract the thick plasma of the upper layer of platelets, and to avoid the extraction of the lower layer, it is necessary to use multiple procedures and tools. (See the above products and videos) in order to successfully complete the extraction of platelet thick plasma.

2. Manual operation is required. After the blood is centrifuged, the subsequent extraction of thick plasma of platelets must be handled manually, which is quite labor intensive.

3. The working time is long. Since the operation procedure for extracting the upper platelet thick plasma is complicated and needs to be performed manually, it takes a lot of time to obtain platelet thick plasma by centrifugation.

4. It is impossible to mass-produce. Since the operation procedure of extracting the upper layer of platelet-rich plasma (Platelet Rich Plasma, PRP) is complicated and needs to be performed manually, it cannot be mass-produced.

In view of the above-mentioned technical deficiencies and needs, the present invention provides a blood filtering device with simplified structure and convenient operation, which not only simplifies the complicated process, but also greatly reduces the steps requiring manual operation, thereby greatly shortening the working time, and the present invention The blood filtration device separates blood by filtration through a hollow filter tube, which is different from the general centrifugal separation technology, and can also be applied to a large amount of platelet-rich plasma separated from blood to achieve mass production.

It is an object of the present invention to provide a blood filtering device.

It is still another object of the present invention to provide a blood filtering apparatus which can separate platelet-rich plasma.

Still another object of the present invention is to provide a blood filtering device for filtering blood using a hollow filter tube.

It is still another object of the present invention to provide a blood filtering device for filtering blood using a metal hollow filter mesh tube.

Still another object of the present invention is to provide a blood filtering device that filters blood using a pressure difference.

It is still another object of the present invention to provide a blood filtering device having an air extracting device.

Still another object of the present invention is to provide a blood filtering device that can filter a large amount of blood.

The blood filtering device of the present invention comprises: a hollow sleeve, an injection tube and a connecting tube are arranged above the hollow sleeve, and a first discharge tube and a second discharge tube are arranged under the hollow sleeve, wherein the injection tube The first discharge valve includes a second valve, the second discharge tube includes a third valve, wherein the injection tube is for injecting blood, and the first discharge tube is for discharging platelet thick plasma, the second The discharge tube is configured to discharge blood cells; a hollow filter tube has a first opening at an upper end and a second opening at a lower end thereof, the hollow filter tube is located inside the hollow sleeve, and the upper end of the hollow filter tube is installed in the hollow a lower end of the injection tube of the sleeve, the first opening of the hollow filter tube is at least partially overlapped with the lower end opening of the injection tube of the hollow sleeve, and the lower end of the hollow filter tube is mounted on the second discharge tube of the hollow sleeve The upper end of the hollow filter tube is at least partially overlapped with the upper end opening of the first discharge tube of the hollow sleeve, wherein the hollow filter tube has a plurality of holes a hole for filtering the blood; and a suction device, wherein the air suction device is coupled to the connecting tube of the hollow sleeve, wherein the air suction device is configured to make the pressure outside the hollow filter tube smaller than that in the hollow filter tube Pressure; wherein the blood filter device filters the blood, the first valve, the second valve and the third valve are in a closed state.

In the above blood filtering device, the hollow sleeve is evacuated by the air suction device, so that the pressure outside the hollow filter tube is smaller than the pressure in the hollow filter tube, and the blood injected into the hollow sleeve is filtered to the hollow by such a pressure difference. The tube flows in the direction of the outside, and the blood is separated and filtered by the pore diameter of the hole in the hollow filter tube, so that platelet rich plasma (PRP) having a diameter smaller than the pore diameter of the hollow filter tube can be filtered to the outside of the hollow filter tube. Flowing into the first discharge tube; blood cells (such as red blood cells, white blood cells) having a diameter larger than the pore diameter of the hollow filter tube are blocked inside the hollow filter tube and flow through the second opening of the hollow filter tube to enter the second discharge tube of the hollow sleeve To achieve the purpose of separating platelet rich plasma (PRP) from blood.

The blood filtering device can pass through a hollow filter tube for filtering blood of humans or different animals, such as human blood, pig blood, bovine blood and the like. The pore size of the hollow filter tube can be selected according to the size of the blood cell of different animals. For example, the diameter of human red blood cells is 6-8 μm, and the diameter of platelets is 2~4 μm, in order to effectively block red blood cells. A hollow filter tube of less than 6 μm, but greater than 4 μm, for example, a hollow filter tube with a pore size of 5 μm can be selected, so that platelet rich plasma (PRP) can smoothly flow into the collection tank through the hollow filter tube.

The above-mentioned so-called injection tube is provided above the hollow sleeve, which means that an injection tube is arranged at the upper end of the hollow sleeve, or an injection tube is arranged laterally near the upper end of the hollow sleeve.

The above-mentioned so-called hollow tube is provided with a connecting pipe, which means that a connecting pipe is arranged at the upper end of the hollow sleeve, or a connecting pipe is arranged laterally of the hollow sleeve near the upper end.

The above-mentioned so-called hollow tube is provided with a first discharge pipe, which means that a first discharge pipe is arranged at the lower end of the hollow sleeve, or a first discharge pipe is arranged laterally of the hollow sleeve near the lower end.

The above-mentioned so-called hollow tube is provided with a second discharge pipe, which means that a second discharge pipe is arranged at the lower end of the hollow sleeve, or a second discharge pipe is arranged laterally of the hollow sleeve near the lower end.

The inner space of the hollow filter tube is hollow, and the position of the first opening of the hollow filter tube is at least partially overlapped with the lower end opening of the injection tube of the hollow sleeve, so that the position of the first opening of the hollow filter tube completely overlaps the hollow sleeve The lower end opening of the injection tube of the cartridge is preferably such that when the blood is filtered, the blood can enter the hollow space inside the hollow filter tube through the injection tube of the hollow sleeve and the first opening of the hollow filter tube. a position of the second opening of the hollow filter tube at least partially overlapping an upper end opening of the second discharge tube of the hollow sleeve, the position of the second opening of the hollow filter tube completely overlapping the upper end of the second discharge tube of the hollow sleeve The opening is preferably such that when the blood is filtered, blood cells (such as red blood cells, white blood cells) having a diameter larger than the pore diameter of the hollow filter tube are blocked inside the hollow filter tube, flow through the second opening of the hollow filter tube, and enter the hollow sleeve. Second discharge pipe.

The hollow filter tube may be any hollow filter tube of any known shape, such as a cylindrical shape, a square column shape, a conical shape, and a fan-shaped column shape, and a cylindrical hollow filter tube is preferred.

The upper end of the full-tube hollow filter tube may further include a first combination portion, which may be coupled to the injection tube of the hollow sleeve in any conventional manner, such as bonding, screwing, locking, and snapping.

The lower end of the full-tube hollow filter tube may further comprise a second combination portion, which may be coupled to the second discharge tube of the hollow sleeve in any conventional manner, such as bonding, screwing, locking, and snapping.

The hollow filter tube may further be provided with a rotating device, so that the hollow filter tube can be rotated in the hollow sleeve to reduce the filter cake generated by the blood cell cells blocking the inside of the hollow filter tube when the blood is filtered.

The hollow filter tube includes a filter portion, and the filter portion can be a filter made of any conventional material, such as a metal mesh, a nylon (NYLON) mesh, or a polypropylene ( PP ) mesh. Polyethylene mesh, polyethylene terephthalate ( PET or PETE) mesh, the above various materials can be made into a single layer or a multi-layer filter. The filter may be a filter made by any conventional method, such as sintering (for metal), non-woven filter (such as PP, PE), plain weave (such as NYLON, PET). Among them, a hydrophilic filter or a metal mesh is preferred.

The hollow filter tube may further include a bracket installed on the inner side of the hollow filter tube for supporting the hollow filter tube, and the bracket may be any conventionally shaped bracket, such as a cylindrical shape, a square column shape, and a polygonal column shape. , tapered, fan-shaped column.

The hollow sleeve may be a hollow sleeve of any shape, such as a cylindrical shape, a square column shape, a polygonal column shape, a tapered shape, and a cylindrical hollow sleeve is preferred. The first valve of the injection tube of the hollow sleeve is used to adjust the flow of blood into the injection tube. The injection tube of the hollow sleeve can be further connected to a continuous blood supply device for continuously delivering blood to the hollow sleeve, and the continuous blood supply device can be a siphonic continuous blood supply device.

The second valve of the first discharge tube of the hollow sleeve is configured to adjust the flow rate of the platelet thick plasma out of the first discharge tube. The third valve of the second discharge tube of the hollow sleeve is configured to adjust the flow rate of blood cells out of the second discharge tube. The connecting tube of the hollow sleeve may further be provided with a fourth valve to adjust the flow rate of the air suction device.

When the blood filtering device filters the blood, the first valve, the second valve and the third valve are in a closed state, so that the platelet-rich plasma can be effectively separated and filtered by the hollow filter tube.

The hollow sleeve may be a detachable hollow sleeve, and the hollow filter tube, the second discharge tube or the injection tube located in the hollow sleeve may be detached or installed by the detachable hollow sleeve.

The blood filtering device may further include a first collecting groove connectable to the first discharge pipe of the hollow sleeve, and the first collecting groove may be a detachable first collecting groove. The first collecting tank may further include a third discharge pipe disposed below or near the side of the first collecting tank for discharging the filtrate in the first collecting tank.

The blood filtering device may further include a second collecting trough connected to the second exhaust pipe of the hollow sleeve, and the second collecting trough may be a detachable second collecting trough. The second collecting tank may further include a fourth discharge pipe disposed below or near the second collecting tank for discharging the filtrate in the second collecting tank.

The above-described air suction device may be any conventional air suction device such as a vacuum pumping device.

10‧‧‧Blood filter

100‧‧‧ hollow sleeve

110‧‧‧Injection tube

111‧‧‧First valve

120‧‧‧First discharge pipe

121‧‧‧Second valve

130‧‧‧Second discharge pipe

131‧‧‧ third valve

140‧‧‧Connecting tube

141‧‧‧fourth valve

210‧‧‧ hollow filter tube

211‧‧‧Filter Department

212‧‧‧ first opening

213‧‧‧ second opening

214‧‧‧First Combination Department

215‧‧‧Second Combination Department

220‧‧‧ hollow filter tube

221‧‧‧Filter Department

222‧‧‧First Combination Department

223‧‧‧Second Combination Department

300‧‧‧Exhaust device

410‧‧‧First collection trough

420‧‧‧Second collection trough

Fig. 1 is a perspective view showing a preferred embodiment of the blood filtering device of the present invention.

Fig. 2 is a schematic cross-sectional view showing a preferred embodiment of Fig. 1.

3 is a perspective view of a hollow filter tube of the preferred embodiment shown in FIG. 1.

4 is a schematic view of another perspective of FIG. 3.

Fig. 5 is a perspective view showing another preferred embodiment of the blood filtering device of the present invention.

Fig. 6 is a schematic cross-sectional view showing a preferred embodiment of Fig. 5.

Figure 7 is a perspective view of the hollow filter tube of the preferred embodiment shown in Figure 5.

The present invention will be further described in detail with reference to the accompanying drawings in which: FIG. 1 is a perspective view of a preferred embodiment of the blood filtering device of the present invention. In the blood filtering device 10, the hollow sleeve 100 is evacuated by the air extracting device 300, so that the pressure outside the hollow filter tube 210 is smaller than the pressure in the hollow filter tube 210, and the pressure difference causes the injection tube 110 to be injected. The blood of the hollow sleeve 100 flows toward the outside of the hollow filter tube 210, and the blood is separated and filtered by the pore diameter of the hole in the hollow filter tube 210 to make the platelet thick plasma having a diameter smaller than the pore diameter of the hollow filter tube 210 (Platelet Rich) Plasma, PRP) may flow into the first collection tank 410 through the hollow filter tube 210 and the first discharge tube; and blood cells (such as red blood cells, white blood cells) having a diameter larger than the pore diameter of the hollow filter tube 210 are blocked in the hollow filter tube 210. Then, through the second discharge tube 130 of the hollow sleeve 100, the second discharge tank 420 flows into the second collection tank 420 to achieve the purpose of separating platelet rich plasma (PRP) from the blood. When the blood filtering device 10 filters blood, the first valve 111, the second valve 121 and the third valve 131 are in a closed state, so that the platelet-rich plasma can be effectively separated and filtered by the hollow filter tube 210.

The first valve 111 of the injection tube 110 of the hollow sleeve 100 is used to adjust the flow of blood into the injection tube. The second valve 121 of the first discharge tube 120 of the hollow sleeve 100 is used to adjust the flow rate of the platelet thick plasma out of the first discharge tube 120. The third valve 131 of the second discharge tube 130 of the hollow sleeve 100 is used to adjust the flow rate of blood cells out of the second discharge tube 131. The hollow sleeve 100 is connected to the fourth valve 141 of the tube 140 to adjust the flow rate of the air extracting device 300.

Fig. 2 is a schematic cross-sectional view showing a preferred embodiment of Fig. 1. The inner space of the hollow filter tube 210 is hollow, and the upper end has a first opening 212 (see FIG. 3). The position of the first opening 212 of the hollow filter tube 210 completely overlaps the lower end opening of the injection tube 110 of the hollow sleeve 100 to filter In the blood, blood can enter the hollow space inside the hollow filter tube 210 via the first opening 212 of the hollow filter tube 210. The position of the second opening 213 (see FIG. 4) of the hollow filter tube 210 completely overlaps the second discharge tube 130 of the hollow sleeve 100, so when the air extracting device 300 starts to operate, when the blood is filtered, the diameter is larger than the hollow filter tube 210. The blood cell (eg, red blood cells, white blood cells) of the upper hole aperture is blocked inside the hollow filter tube 210, flows through the second opening 213 of the hollow filter tube 210, and enters the second discharge tube 130 of the hollow sleeve 100.

3 is a perspective view of a hollow filter tube of the preferred embodiment shown in FIG. 1. The filter portion 211 of the hollow filter tube 210 is a metal mesh, and the size of the mesh is larger than the diameter of the platelet thick plasma (PRP) and smaller than the diameter of the blood cell (such as red blood cells, white blood cells). The position of the first opening 212 of the hollow filter tube 210 at least partially overlaps the injection tube 110 of the hollow sleeve 100. The first combination portion 214 of the hollow filter tube 210 is screwed to the top of the hollow sleeve 100; the second combination portion 215 of the hollow filter tube 210 is screwed to the bottom of the hollow sleeve 100.

4 is a schematic view of another perspective of FIG. 3. The position of the second opening 213 of the hollow filter tube 210 at least partially overlaps the second discharge tube 130 of the hollow sleeve 100.

Fig. 5 is a perspective view showing another preferred embodiment of the blood filtering device of the present invention. In the blood filtering device 10, the hollow sleeve 100 is evacuated by the air extracting device 300, so that the pressure outside the hollow filter tube 220 is smaller than the pressure in the hollow filter tube 220, and the pressure difference causes the injection tube 110 to be injected. The blood of the hollow sleeve 100 flows toward the outside of the hollow filter tube 220, and the blood is separated and filtered by the pore diameter of the hole in the hollow filter tube 220 to make the platelet thick plasma having a diameter smaller than the pore diameter of the hollow filter tube 220 (Platelet Rich) Plasma, PRP) can flow into the first collection tank 410 through the hollow filter tube 220 and the first discharge tube 120; and blood cells (such as red blood cells and white blood cells) having a diameter larger than the pore diameter of the hollow filter tube 220 are blocked in the hollow filter tube. In 220, the second discharge tube 130 of the hollow sleeve 100 flows into the second collection tank 420 to achieve the purpose of separating platelet rich plasma (PRP) from blood.

Fig. 6 is a schematic cross-sectional view showing a preferred embodiment of Fig. 5. The inner space of the hollow filter tube 220 is hollow, the injection tube 110 passes through the top surface of the hollow sleeve 100 and is connected to the top of the hollow filter tube 220, and the second discharge tube 130 passes through the bottom surface of the hollow sleeve 100 and is connected to the hollow filter tube. The bottom of the 220.

Figure 7 is a perspective view of the hollow filter tube of the preferred embodiment shown in Figure 5. The filter portion 221 of the hollow filter tube 220 is a metal mesh. The injection pipe 110 is connected to the first combination portion 222 of the hollow filter tube 220, and the second discharge pipe 130 is connected to the second combination portion 223 of the hollow filter tube 220.

Claims (10)

 A blood filtering device comprises: a hollow sleeve, an injection tube and a connecting tube are arranged above the hollow sleeve, and a first discharge tube and a second discharge tube are arranged below the hollow sleeve, wherein the injection tube comprises a The first valve, the first discharge tube comprises a second valve, and the second discharge tube comprises a third valve, wherein the injection tube is for injecting blood, the first discharge tube is for discharging platelet thick plasma, and the second discharge tube is for discharging a hollow filter tube having a first opening at an upper end and a second opening at a lower end thereof, the hollow filter tube being located inside the hollow sleeve, the upper end of the hollow filter tube being mounted on the hollow sleeve The injection tube has a first opening at least partially overlapping the lower end of the injection tube of the hollow sleeve, and the lower end of the hollow filter tube is mounted on the second discharge tube of the hollow sleeve, the hollow filter a position of the second opening of the tube at least partially overlapping the upper end of the first discharge tube of the hollow sleeve, wherein the hollow filter tube has a plurality of holes for filtering blood; a gas device, wherein the air suction device is coupled to a connecting tube of the hollow sleeve, wherein the air suction device is configured to make a pressure outside the hollow filter tube smaller than a pressure in the hollow filter tube; wherein the blood filtering device filters the blood The first valve, the second valve and the third valve are in a closed state.    The blood filtering device according to claim 1, wherein the hollow filter tube has a pore diameter larger than a platelet diameter and smaller than a red blood cell diameter.    The blood filter device of claim 2, wherein the hollow filter tube comprises a filter portion made of a metal mesh, a nonwoven mesh or a filter cloth.    The blood filtering device according to claim 3, wherein the filter portion of the hollow filter tube is made of a single layer or a plurality of metal meshes, a nonwoven mesh or a filter cloth.    The blood filter device of claim 4, wherein the filter portion of the hollow filter tube is made of a multilayer metal mesh.    The blood filtering device of claim 3, wherein the hollow filter tube further comprises a bracket for supporting the hollow filter tube.    The blood filtering device of claim 1, further comprising a rotating device, wherein the rotating device is coupled to the hollow filter tube to rotate the hollow filter tube in the hollow sleeve.    The blood filtering device of claim 1, wherein the blood filtering device further comprises a continuous blood supply device, wherein the continuous blood supply device is coupled to an injection tube of the hollow sleeve, and the continuous blood supply device is configured to continuously deliver blood. To the hollow sleeve.    The blood filtering device of claim 1, wherein the hollow sleeve is detachable for replacing the hollow filter tube installed in the hollow sleeve.    The blood filter device of claim 1, wherein the connecting tube of the hollow sleeve further comprises a fourth valve.