Λ 6 Π 6 201687 五、發明説明（3) 發明範畴 本發明係關於抗微生物组成物及控制微生物之方法。 先前技術之敘述 在各種水性糸统，如乳液，漆料，塗裝，冷卻水糸統 ，裝飾池等裡面存在之撖生物可造成此等条統之品質惡化 或變得難看。例如，經上漆之表面可能由於徹生物無法査 覺之生長而變難看，因此損壊經上漆物件之整體美；冷卻 塔亦可能由於表面微生物的成長而喪失其效率，因此降低 冷卻塔的熱傳能力。一般實用的方法僳藉由加入各種添加 劑或結合具有抗徹生物活性持性之添加劑，以抑制此条統 微生物之惡化。 在不同之環境中營經用於控制黴生物之各種不同之物 質，其中包括：氣/溴化合物，戊二薛（glutaraldehyde), 異I*唑酮，有機錫配方，銅塩，四级銨化合物（SD，Λ 6 Π 6 201687 V. Description of the invention (3) Scope of the invention The present invention relates to antimicrobial compositions and methods for controlling microorganisms. Description of the prior art The living organisms present in various water-based systems, such as emulsions, paints, coatings, cooling water systems, decorative pools, etc., can cause the quality of these systems to deteriorate or become unsightly. For example, the painted surface may become unsightly due to growth that cannot be detected by the organism, so the overall beauty of the painted object may be impaired; the cooling tower may also lose its efficiency due to the growth of surface microorganisms, thus reducing the heat of the cooling tower Transmission ability. The general practical method is to suppress the deterioration of microorganisms by adding various additives or combining additives with anti-biological activity. Various substances used to control mold organisms in different environments, including: gas / bromine compounds, glutaraldehyde, iso I * oxazolone, organotin formulations, copper bases, quaternary ammonium compounds (SD,
Strauss and PR Puckorius in J . Power, SI, June 1984)，及三畊。每一種均具有關於毒性，PH及溫度敏感 性，有限之效率，化學安定性，及/或相容性之缺點。 二甲基二硫代胺基甲酸鐵為已知之農業用殺真菌劑。 基於上述一般抗徹生物化合物效能之缺乏，需要更有效的 抗微生物劑，此抗撤生物劑可在較低的配藥率下使用，因 此對最终使用而言可以有最有效的成本，降低對環境条统 污染的負載，且降低對附近非目標之有機髏之副作用，如 魚，有用的穀物，等。 發明概要____ 本紙張尺度逍用中圉國家標準（CNS)甲4規格（210x297公没） 81. 2. 20,000 3 (請先閱讀背而之注意事項洱蜞寫本頁) 裝. 經濟部屮央櫺準局员工消赀合作杜印製 Λ 6 η 6 201687 五、發明説明（4) (請先閲讀背而之注意事項#艰寫本頁； 本發明之一目的偽提供一種在相當低量之活性成份下 控制微生物之方法。本發明之另一目的偽使用與容易受到 微生物損壊之各種条統相容之組成物。本發明之另一目的 係提供一種方法用於控制冷卻塔，漆料，海中之防污塗料 ，噴佈洗液，游泳池，塗料，裝飾池，織物，皮革，紙， 木材，金羼加工液，燃料糸統等中之微生物，且對經處理 (及經控制）之条統沒有討厭之副産物臭味，變色，或其他 的有害影逛。此等目的，及自下列之掲示而變成顯而易見 之其他目的可藉本發明而完成，本發明傺一種用於控制撤 生物之組成物，包括（Α)4,5 -二氛-2 -正辛基-3-異1¾唑酮 或2 -甲基-3-異速唑酮，及（B)二甲基二硫代胺基甲酸鐵， 其相互間之比例傺可顯示協合者。 本發明之其他目標包括一種用於抑制選自包含細菌， 真菌，藻類及其混合物之菌類於易受該菌類污染之場所之 生長之方法，包括在場所中加人有效量之上述組成物，以 有效的抑制該菌類之成長。 本發明之其他目標偽一種控制冷卻塔撤生物之方法， 經濟部屮央標準局A工消伢合作社印奴 包括維持上述組成物在水中之濃度。 本發明亦包括抗徹生物塗料或浸漬組成物，及包括此 抗徹生物組成物之海上防污組成物。 發明之詳細説明及較佳具體例 吾人曾經發現一種用於控制撤生物待別有效的組成物 ，包括（A ) 2 -甲基-3 -異I*唑酮，或4，5 -二氛-2 -正辛基-3-異1®唑酮，及（B)二甲基二硫代胺基甲酸鐵，其相互間之 81. 2. 20,000 本紙?尺度边用中國困家標準（〔奶）肀<1規格（210x297公没） Λ 6 Β 6 201687 五、發明説明（5) 比例偽可顯現協合者。 (請先閲請背而之注意事項再填寫本頁〕 依據本發明，提供一種控制微生物之方法包括使用有 效量之上述組成物。 其他重要之用途係對塗料或浸漬組成物賦與對微生物 之抗性，包括在塗料或浸漬组成物中加入本發明之组成物 ,較好濃度約為O.lppm至約2百分比，且濃度約為lppm至 約1百分比更好，且最好濃度約為10至4000ppm。 本發明所提供之抗藻類塗料或浸漬組成物較好包括約 O.lppia至約2百分比之抗微生物組成物，且更好約10至 4000ppm〇 另一方面，在海上防污組成物中，本發明之抗微生物 組成物包括防污組成物之約1至10百分比。 經濟部屮央櫺準局β工消枰合作杜印奴 在金羼加工液之處理中；及在織物，皮革、紙或木材 物質之保護中，殺微生物劑組成物之添加濃度約為Ο.Ιρρπ 至約2重量百分比。在水性介質中，殺撤生物劑組成物包 括水性糸統之約O.lppin至約1百分比，傜依持定之最终用 途而定；例如，在冷卻水塔用途及紙漿或製紙程序中，殺 微生物劑組成物之添加濃度自約0.1至約ΙΟΟΟρρπ(重量）。 在燃料条統之處理中，例如，噴射燃料，汽油，加熱油， 等等，殺微生物劑組成物之添加濃度自約O.lppm至約1重 量百分比。 微生物抗性組成物亦可用於建築産物，如粉飾用灰泥 、屋頂灰泥、牆壁灰泥、及用於藻類保護之石造建築塗料 ；及用於保護基礎基材之透明修飾及塗裝免受藻類污染； 本紙法尺度边用中國國家標準（CNS)肀4規格（210x297公犮） 81. 2. 20,000 201687_ 五、發明説明<g ) 經濟部屮央捣準局ex工济设合作杜印5i 池及 蝦聞 小休 ，之 所水 卵於 孵用 類 ； 魚育 、 培 魚之 養物 含動 包類 , 毅 制甲 控與 類物 藻動 之髏 中軟 耕， 水池 於魚 用， .， 或 池降 飾沈 裝如 及 ， 池制 水控 噴類 .0 湖之 , 中 池體 泳水 游之 如用 ，使 制上 控區 之地 類或 藻用 中業 體工 飾於 裝用 控 ； 類制 藻控 之之 業類 農藻 耕中 水程 於過 用造 ; 製 槽及 水工 儲加 及品 , 産 池 類 理紙 處及 水漿 廢紙 ,於 池用 離 .， 分制 游 於 用 及 染 污 之 類 藻 受 免 。 品料 料塗 塑及 之料 料塑 塗之 或中 料膠 塑 塑 含裡 包襯 於池 用泳 約 ο 比10。 量5/定 重0.而 之自酮 B)傺唑 圍DS 範異 U率之 中 比 用 其之使 ，好依 中別 ， 物待10 成 。1/ frr{ 1 绍 . 努 物/0至 生0010 二1/Μ ' 之 ο , 佳10/1 較1/10 ο 約 為至 (价先閲讀背而之注意事項界填寫本叉- 對 〇 化 物變 合 ， 化良 及改 途的 用種 種各 多 C 些明 某發 明本 發制 本限 表不 代但 僅明 僅說 例於 實用 之傜 列例 下實 等 此 明 發 本 屬 都 應 且 的 楚 清 是 該 應 者 術 技 。 本内 習之 熟圍 對 範 善及 改神 及精例 , 之實 驟 步 般 壓 /IV 制 抑 全 完 〇 需物 像 合 義化 定之 此量 〇 低 度 最 濃之 制需 抑所 之長 小成 最體 示機 表有 值之 1C定 Μ給 \—/ 抑 之 獨 單 C 份F. 成以 二 ， 於究 大研 傜之 , 合 鎏結 影物 之合 同化 不種 種二 二 自 指係 係用 應作 效合 合 協 協 。 合 總 ο Γ 2_, er來 ay法 Μ方 Κ.算 R 計 及之 CZ述 W1敘 裝· 訂_ 線 量 測 本紙張尺度边用中a 8家楳準(CNS)甲4規格（210X297公;a：) 81. 2. 20,000 9.01687 Λ 6 Π 6 經濟部屮央櫺準局員工清伢合作社印奴 五、發明説明（7 ) Q a Q b + = 協合作用指數 （SI) Q a Q B 其中： Qa =化合物A單獨作用之量，所得之終點（MIC) Qa=化合物A混合作用之量，所得之終點（MIC) Qb =化合物B單獨作用之量，所得之終點（MIC) Qb =化合物B混合作用之量，所得之終點（MIC) 下列之S I值可達到： S I > 1 表示對抗效應 S I = 1 表示加成效應 SI< 1 表示協合作用 效率之研究僳在各種微生物有機體中，以二甲基二硫 代胺基甲酸鐵及異1«唑酮混合物進行。MIC之研究係使用 微滴定板分析進行。在此方法中，廣範圍濃度係藉著於96 -洞塑膠微滴定板中製備化合物之二倍連鑛稀釋而測試， 所有液體介質之轉移均以已校正之單一或多槽數位吸量管 進行。化合物之儲存溶液係在適當之溶劑中製備，且施於 生長介質中。在板中所有隨後之稀釋均使用所需之生長介 質製成；在每一洞中之液體總體積為ΙΟΟ/ul。每一板均包 含在徹滴定板中以二方向連缅滴定等體積之液體所製成之 二化合物之濃度。每一板均包含用於每一組合之對照列（ 僅單一成份），因此，亦可測量艏別化合物之Μ I C值。 Β .異噬唑結權_ (請先閲讀背而之注意事項#蜞寫本孓 裝. 訂 線_ 本紙张尺度逍用中S困家標準(CNS)甲4規格（210x29/公Λ) 81. 2. 20,000Strauss and PR Puckorius in J. Power, SI, June 1984), and three tillage. Each has disadvantages with regard to toxicity, pH and temperature sensitivity, limited efficiency, chemical stability, and / or compatibility. Ferric dimethyldithiocarbamate is a known agricultural fungicide. Based on the above lack of effectiveness of general anti-biological compounds, a more effective anti-microbial agent is required. This anti-biotic agent can be used at a lower dispensing rate, so it can have the most effective cost for the final use and reduce the environmental impact Loads of conventional pollution, and reduces side effects on nearby non-target organic skeletons, such as fish, useful grains, etc. Summary of the invention____ This paper scale is used in the Chinese National Standard (CNS) A4 specification (210x297 public) 81. 2. 20,000 3 (please read the precautions written on this page first). Installed by the Ministry of Economic Affairs The quasi-bureau employees of the Central Bureau of Puritan Cooperation cooperated to produce Λ 6 η 6 201687. V. Description of the invention (4) (please read the back-end notes first # Difficult to write this page; one of the purposes of the present invention is to provide a very low volume The method of controlling microorganisms under the active ingredients. Another object of the present invention is to use a composition compatible with various systems that are easily damaged by microorganisms. Another object of the present invention is to provide a method for controlling cooling towers and paints. , Antifouling paints in the sea, spray lotion, swimming pools, paints, decorative ponds, fabrics, leather, paper, wood, gold processing fluids, fuel systems and other microorganisms, and the treated (and controlled) There are no unpleasant by-products such as odor, discoloration, or other harmful shadows. These purposes, as well as other objects that become obvious from the following description, can be accomplished by the present invention. group Products, including (Α) 4,5-dihydro-2-n-octyl-3-iso-1,2-oxazolone or 2-methyl-3-isodyzazolone, and (B) dimethyl dithioamine Ferrocarbamate, the ratio of which can show synergies. Other objects of the present invention include a method for inhibiting the growth of fungi selected from bacteria, fungi, algae and mixtures in places susceptible to contamination by the fungi The method includes adding an effective amount of the above-mentioned composition to the place to effectively inhibit the growth of the fungus. Other targets of the present invention are a method of controlling the withdrawal of the cooling tower, A Gongxiaoji Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Innu includes maintaining the concentration of the above composition in water. The present invention also includes anti-biological coating or impregnating composition, and marine anti-fouling composition including the anti-biological composition. Detailed description of the invention and preferred specific examples A composition that has been found to be effective for controlling withdrawal is included, including (A) 2 -methyl-3 -iso I * azolone, or 4,5-dihydro-2-n-octyl-3-iso 1 ® oxazolone, and (B) ferric dimethyldithiocarbamate, their mutual 81. 2. 20,0 00 This paper? The standard uses the Chinese standard for trapped families ([milk] 肀 < 1 specification (210x297 gong)) Λ 6 Β 6 201687 V. Description of the invention (5) The proportion of pseudo can show the collaborator. (Please read the back first Please pay attention to this page and fill out this page] According to the present invention, a method for controlling microorganisms is provided, which includes the use of an effective amount of the above-mentioned composition. Other important uses are to impart resistance to microorganisms to paints or impregnated compositions, including in paints. Or the composition of the present invention is added to the impregnation composition, preferably the concentration is about 0.1 ppm to about 2 percent, and the concentration is preferably about 1 ppm to about 1 percent, and the most preferable concentration is about 10 to 4000 ppm. The anti-algae coating or impregnating composition provided by the present invention preferably includes about 0.1 to about 2 percent of antimicrobial composition, and more preferably about 10 to 4000 ppm. On the other hand, in the marine antifouling composition, the present The inventive antimicrobial composition includes about 1 to 10 percent of the antifouling composition. The Ministry of Economic Affairs, the Ministry of Economic Affairs, the Central Bureau of Labor and Social Security, and the cooperation of Du Yinnuo in the treatment of Jinyi processing fluid; and in the protection of fabrics, leather, paper or wood substances, the concentration of the microbicide composition is about Ο. Ιρρπ to about 2 weight percent. In aqueous media, the biocide composition includes about 0.1 lppin to about 1 percent of the water-based system, depending on the end use it holds; for example, in the use of cooling towers and pulp or paper making procedures, microbicides The added concentration of the composition is from about 0.1 to about 100 ρρπ (weight). In the treatment of fuel systems, for example, fuel injection, gasoline, heating oil, etc., the concentration of the microbicide composition is from about 0.1 ppm to about 1 weight percent. Microbial resistant compositions can also be used in construction products, such as stucco for plastering, roof stucco, wall stucco, and stone architectural coatings for algae protection; and for transparent modification and coating of basic substrates from Algae pollution; the Chinese standard (CNS) 4 specifications (210x297 gong) are used on the scale of this paper method 81. 2. 20,000 201687_ V. Description of the invention < g) Ex-industrial cooperation cooperation of the Ministry of Economic Affairs 5i Ponds and shrimps take a short break, and the eggs are used for hatching; the nutrients of fish breeding and fish culture include kinetics, soft-cultivation in the skeletal body of the control system and algae, and the ponds are used for fish. ., Or pool descending decoration as well as, pool water control spray type. 0 Lake, the swimming pool swimming pool is used as appropriate, so that the land or algae in the upper control area are decorated with Chinese industrial equipment. Use control; the industry of algae production and control, the agricultural process of algae cultivation is over-utilized; the trough and hydraulic storage and addition products, the paper-pool sorting place and the water-waste paper are used in the pool. Algae that are used separately and contaminated are exempted. The material is coated with plastic and the material is coated with plastic or the plastic is plastic with plastic lining. Amount of 5 / fixed weight 0. And from the ketone B) yezo around the DS range of the U rate is better than using it, according to the difference, it will be 10%. 1 / frr {1 Shao. Nu Wu / 0 to Sheng 0010 II 1 / Μ ', ο, 10/1 is better than 1/10 ο about to (the price first reads the precautions to fill out this fork-yes 〇 There are many different kinds of chemical compounds, good ones and improved ways. It is clear that a certain invention does not replace the limitations of this invention, but it only states that the examples are only under the practical examples of the enlightenment. The Chu Qing is the skill of the responder. The familiarity of this internal practice is a step-by-step press of Fan Shan and the improvement of the gods and fine examples. IV suppression is complete. The amount of synonymization of the object is low. The strongest control system requires the length of the restraint to become the most representative machine. The value of 1C is given to the M — \ / Yizhi alone C part F. In two, in the research of the research, together with the photo The contractualization of things does not mean that the two-two self-referential system should be used as a synergy. The total ο Γ 2_, erlai ay method Μ 方 Κ. Calculate R CZ accounted for W1 narration · order _ quantity The paper size of the test paper is used in a 8 specifications (CNS) A 4 specifications (210X297; a :) 81. 2. 20,000 9.01687 Λ 6 Π 6 Employee of the Ministry of Economic Affairs Cooperative Indies V. Description of invention (7) Q a Q b + = Synergy index (SI) Q a QB where: Qa = the amount of compound A acting alone, and the end point (MIC) obtained Qa = compound A mixing Amount, the end point (MIC) obtained Qb = the amount of compound B acting alone, the end point (MIC) obtained Qb = the amount of compound B acting, the end point obtained (MIC) The following SI values can be achieved: SI > 1 Represents the antagonistic effect SI = 1 Represents the additive effect SI < 1 Represents the study of the synergistic effect. In various microbial organisms, a mixture of ferric dimethyldithiocarbamate and iso 1 oxazolone was used. MIC study It is analyzed using a microtiter plate. In this method, a wide range of concentrations is tested by preparing a double-concentration dilution of the compound in a 96-hole plastic microtiter plate, and the transfer of all liquid media is calibrated as a single Or a multi-channel digital pipette. The storage solution of the compound is prepared in a suitable solvent and applied to the growth medium. All subsequent dilutions in the plate are made using the required growth medium; in each hole Liquid The total volume is 100 / ul. Each plate contains the concentration of the two compounds made by titrating the same volume of liquid in two directions in a thorough titration plate. Each plate contains a control column for each combination ( Only a single component), so you can also measure the MIC value of the other compounds. Β. Isoprazole knot power _ (please read the back of the note first # 蜞 写 本 孓 装. Threading _ This paper standard is easy to use in the S home standard (CNS) A 4 specifications (210x29 / Gong Λ) 81. 2. 20,000
*? a c 42C 6 6 An 五、發明説明（g ) 包含於本實例中之異α唑酮命名如下：*? a c 42C 6 6 An V. Description of the invention (g) The iso-α-zazolones included in this example are named as follows:
經濟部屮央標準局员工濟#合作社印M 1 .異噬 唑 酮A : 4, 5 -二氣-2- 正辛基-3-異 «唑酮 2 .異睡 唑 酮B : 2 -甲基-3 -異 睡唑酮 實例1 • • 使 用 A s p e r g i 1 1 u s n i g e Γ純的培餐基 ，二甲基二硫 胺基甲 酸鐵及異β唑酮A之各種結合僳在 ycophil bro 中進行Μ I C測量 。化合物a為異α唑酮a, 且化合物B為 基二硫 代 胺基甲酸鐵。 £La_ Q b Q a / Q b Si 0 64 (Qb) - 1.0 0.16 32 1/205 0.56 0.16 16 1/99 0.31 0.16 8 2/98 0.19 0.31 • 32 1/99 0.62 0.31 16 2/98 0.37 0.31 8 4/96 0.25 0.31 4 7/93 0.19 0.31 2 14/86 0.16 0.62 32 2/98 0.75 0.62 16 4/96 0.5 0.62 8 7/93 0.37 0.62 4 14/86 0.31 0.62 2 24/76 0.28 0.62 1 ·? 8 / R ? 0-27 本紙張尺度遄用中困困家樣準（CNS)肀4規格（210x297公;«：) (ir先閲讀背而之注意事項#-堝寫本又 裝· 線. 81. 2. 20,000 8 201687 Λ 6 Π 6 經濟部中央標準局员工济t合作杜印t 五、發明説明（g ) 0.62 0 . 5 56/44 0.26 1.25 16 7/93 0.75 1.25 a 14/86 0.62 1.25 4 24/76 0.56 1.25 2 38/62 0.53 1.25 1 56/44 0.52 1.25 0.5 7/29 0.51 1.25 0.25 83/ 1 7 0.5 2.5 (Qa) 0 - 1.0 實例2 : 使用Ps eudomonas a eruginosa純的培養基 ,二甲基 硫代按基甲 酸鐵及異α唑_B2各種结合偽在B y c o p h i 1 broth中進行MIC之測量。 化合物A為異《唑酮B ,且化合 B為二甲基二硫代胺基甲 酸鐵。 QLa Q b Q Λ / Q h S_L 0 7 5 00 (Qb) - 1.0 16 125 11/89 <0.35 16 64 20/80 <0.63 16 32 33/67 <0.56 16 16 50/50 <0.53 16 8 67/33 <0.52 16 4 80/20 <0.51 16 2 89/11 <0.51 32 (Qft) 0 - 1 0 本紙5良尺度边用中a Η家樣準(CNS)肀4規格（210X297公龙） 81. 2. 20,000 9 (諫先閲讀背而之注意事項祚填寫本>· 裝· 訂Employee Ji # Cooperative Society of the Ministry of Economic Affairs Standards Bureau Co., Ltd. M 1. Isopazolone A: 4, 5 -Digas-2-n-octyl-3-iso «azolone 2. Isozonazolone B: 2-A -3-Isozoazolone Example 1 • • Use A spergi 1 1 usnige Γ pure meal base, various combinations of iron dimethyldithiocarbamate and isopazolone A in ycophil bro IC measurement. Compound a is isoalphazodone a, and compound B is iron dithiocarbamate. £ La_ Q b Q a / Q b Si 0 64 (Qb)-1.0 0.16 32 1/205 0.56 0.16 16 1/99 0.31 0.16 8 2/98 0.19 0.31 • 32 1/99 0.62 0.31 16 2/98 0.37 0.31 8 4/96 0.25 0.31 4 7/93 0.19 0.31 2 14/86 0.16 0.62 32 2/98 0.75 0.62 16 4/96 0.5 0.62 8 7/93 0.37 0.62 4 14/86 0.31 0.62 2 24/76 0.28 0.62 1 8 / R? 0-27 The size of this paper adopts the CNS standard 4 (210x297 g; «:) (ir first read the notes on the back # -cooker and reinstall the line. 81 . 2. 20,000 8 201687 Λ 6 Π 6 Employee cooperation of the Central Bureau of Standards of the Ministry of Economic Affairs Du Yint 5. Invention description (g) 0.62 0. 5 56/44 0.26 1.25 16 7/93 0.75 1.25 a 14/86 0.62 1.25 4 24/76 0.56 1.25 2 38/62 0.53 1.25 1 56/44 0.52 1.25 0.5 7/29 0.51 1.25 0.25 83/1 7 0.5 2.5 (Qa) 0-1.0 Example 2: Use Ps eudomonas a eruginosa pure medium, two The measurement of MIC in B ycophi 1 broth was carried out with various combinations of methylthiothiocarbamate and isoαazole_B2. Compound A is isooxazolone B, and compound B is dimethyl disulfide Iron carbamate QLa Q b Q Λ / Q h S_L 0 7 5 00 (Qb)-1.0 16 125 11/89 < 0.35 16 64 20/80 < 0.63 16 32 33/67 < 0.56 16 16 50 / 50 < 0.53 16 8 67/33 < 0.52 16 4 80/20 < 0.51 16 2 89/11 < 0.51 32 (Qft) 0-1 0 CNS) Yuan 4 specifications (210X297 male dragon) 81. 2. 20,000 9 (Remember to read the precautions first, fill in this book > · Install · Order