TW201444465A - Method of monitoring fertilizer absorptivity of plant - Google Patents

Abstract

A method of monitoring fertilizer absorptivity of plant, comprising the following steps: (A) inserting two needle electrodes respectively into the stem and leaf vein of a plant; (B) adding liquid fertilizer to the cultivation medium around the plant root; and (C) using the electrodes to perform redox potential scanning on the plant and obtaining an oxidation current data. By inserting the electrodes on the plant to perform redox potential scanning, after the addition of fertilizer, the absorption of fertilizer and nutrient by plant can be realized to greatly shorten the time required for fertilizer research and development.

Description

Method for monitoring plant absorption of applied fertilizer

The present invention relates to a method of monitoring plants, and more particularly to a method of monitoring the absorbance of a plant for the applied fertilizer.

In order to effectively boost agricultural development, the government and non-government units are all actively investing in scientific and technological agriculture in an attempt to increase agricultural production and quality through scientific methods to produce high-quality agricultural products such as rice, fruits or vegetables. At present, the most common one is the improvement of the variety or the improvement of the fertilizer. In the application of fertilizer, the main focus is on which fertilizer should be applied in different growth stages of the crop to effectively promote the growth of the crop, but in the end the crop is applied to the fertilizer. Whether there is real absorption, can only be judged by observing the growth of crops, it is quite time-consuming, and it is impossible to monitor the absorption of the applied fertilizer by the plant immediately, and adjust the type of fertilizer or the type of fertilizer applied immediately.

Accordingly, it is an object of the present invention to provide a method for instantly monitoring the absorbance of a plant for application of the fertilizer.

Thus, the method of the present invention for monitoring the absorbance of a plant for the applied fertilizer comprises the steps of: (A) inserting two needle-shaped electrodes separately a stem of a plant and a leaf vein; (B) applying a liquid fertilizer to a culture medium surrounding the root of the plant; and (C) performing a redox potential scan on the plant via the electrodes, and obtaining a redox Current data.

Thus, the method for monitoring the absorbance of a fertilizer applied by a plant comprises the steps of: (A) inserting two needle-shaped electrodes into a stem of a plant and a liquid culture medium for cultivating the plant; B) applying a liquid fertilizer to the liquid culture medium around the root of the plant; and (C) scanning the plant for oxidation-reduction potential via the electrodes, and obtaining a redox current data.

The effect of the invention: by inserting the electrodes on the plant and performing the oxidation-reduction potential scanning method, the change of the absorption of the fertilizer nutrients of the plant can be quickly evaluated by the change of the redox current immediately after the application of the fertilizer. Helps shorten the time course of fertilizer development research.

3‧‧‧Electrode

4‧‧‧ plants

6‧‧‧Electrochemical Analyzer

Other features and advantages of the present invention will be apparent from the embodiments of the present invention, wherein: FIG. 1 is a flow chart showing the steps of a preferred embodiment of the method for monitoring plant absorbance of applied fertilizer; Figure 2 is a schematic view showing the insertion of two electrodes when the preferred embodiment is applied to the plant "Burmese"; Figure 3 is a schematic view showing the insertion of two electrodes when the preferred embodiment is applied to the "pocket" of the plant; It is a graph showing the change of redox current measured when the micro-lipid nutrient solution and water are applied to the plant "Mulberry" respectively; Fig. 5 is a graph showing changes in redox current measured when a microlipid nutrient solution and water were applied to a plant "cup".

As shown in Figures 1, 2 and 3, the preferred embodiment of the method for monitoring the absorbance of a plant for application of the fertilizer is applied to plants 4 such as herbaceous plants and woody plants, and the redox reaction of the plant 4 is monitored. The magnitude of the change is used to assess and monitor the absorption of the applied liquid fertilizer by the plant 4. In the following examples, the succulent plant "shou" and the oleander family "Mulberry" are described as an example.

The method of the present invention for monitoring the absorbance of a plant for the applied fertilizer comprises the steps of: (a) inserting an electrode 3 into the plant 4.

In the present invention, the needle electrode 3 is used. In the present embodiment, a stainless steel (SS316) acupuncture needle for acupuncture is used as the electrode 3, and the electrode 3 has a diameter of 0.24 mm.

Since the leaves of the plant 4 will carry out light and action, and the root system will also contact the soil and undergo redox reaction to take up nutrients, the inside of the plant 4 is originally a redox reaction tank, and the redox reaction continues. And depending on the amount of nutrients absorbed, different magnitudes of redox current changes occur. Therefore, the case is inserted into the plant 4 or inserted into the culture medium of the plant 4 and the cultured plant 4, and the plant 4 is monitored. The internal redox current trends tend to monitor the absorption of the applied fertilizer by the plant 4 to further assess the effect of the applied fertilizer on the growth of the plant 4.

For the Burmese flower, two electrodes 3 are inserted in the veins of one of the leaves and inserted into the stem, and the length of the electrode 3 is 0.1 cm. For the pocket, one of the electrodes 3 is inserted into the stem of the pocket, the insertion length is 0.1 cm, and the other electrode 3 is inserted into the culture medium of the planting pocket, and the distance between the two electrodes 3 is 10 cm or more. In the present embodiment, the distance between the two electrodes 3 is 10 cm. However, when implemented, the depth of insertion of the electrode needles into the plant 4 can be adjusted according to different plant species.

In the present embodiment, the culture medium of the pocket is in a liquid state, the root portion thereof is immersed in the liquid culture medium, and the culture medium of the Burmese flower is soil, but in practice, the suitable plant 4 species are not limited to the above species, and The culture medium is not limited to the above two.

After the electrode 3 is inserted, the electrodes 3 are electrically connected to a cyclic voltameter 6, respectively. For the Burmese flower, the electrode 3 inserted in the vein is used as a working electrode, and is inserted into the stem. The other electrode 3 of the portion serves as a counter electrode and a reference electrode. For the pocket, the electrode 3 inserted in the stem portion is used as the working electrode, and the other electrode 3 inserted in the culture medium is used as the reference electrode and the counter electrode. And the electrode 3 is left on the plant 4 throughout the experimental procedure.

Step (2) applies liquid fertilizer to the plant 4.

A predetermined amount of liquid fertilizer and water are separately poured into the culture medium of the plant 4 to be planted. In the present embodiment, the liquid fertilizer applied to the Burmese flower and the pocket is a lipotrophic nutrient solution (Huabao No. 5, Taihe Horticulture Enterprise Co., Ltd.).

In this embodiment, the above two plants 4 are divided into experiments. There were four experimental groups and four control groups in each plant 4, and the same variety of plants 4 were planted in pots under the same planting conditions and placed in an outdoor environment for cultivation. The group will apply the liposome nutrient solution, and the control group will only apply the general tap water. The lipid group nutrient solution was applied to the experimental group every 10 days, and the water was applied to the plant 4 of the control group, and applied once every 10:00 in the morning and 4:00 in the afternoon, each time 1000 ml, wherein the vesicles were The nutrient solution concentration is 100 ppm.

Step (3) performs a redox potential scan and obtains a redox current data.

The oxidation-reduction potential scan was started five minutes after each application of the fertilizer and water. The voltammetric apparatus 6 is used to scan the redox potential of the Burmese flower and the pocket, and analyze the obtained data of oxidation current and reduction current, and use the measured data to evaluate the application of water and microlipid nutrient solution in the plant 4. The subsequent redox reaction changes.

In this embodiment, the scanning potential of the pocket is -1.8~2.5 V, the scanning rate is 0.1 V/sec, the scanning potential range of the Burmese flower is between -2 and 2 V, and the scanning rate is 0.1 V/. Sec. In order to avoid damage to plants caused by excessive oxidation-reduction potential, in the present embodiment, the maximum oxidation-reduction potential applied is in the range of ±2.5 V.

Referring to Table 1 and Figure 4, according to the redox current data measured by the above method, for the Burmese flower, in the case of only adding water, no significant redox current appears, indicating that the plant 4 is applied to water, and the inside thereof The redox reaction is rather weak. On the contrary, the redox current measured after applying the liposome nutrient solution will change significantly, showing the microlipotrophic nutrient solution. The application will drive a vigorous redox reaction inside the plant 4, and it is estimated that the absorption of the microlipid nutrient solution by the plant is relatively fast and the absorption is improved.

With the increase of planting days, the oxidation current and reduction current of the microlipid nutrient solution will increase significantly, and the outer diameter of the stem of the Burmese flower will also change from 3.2 cm on the first day to 3.8 cm on the 41st day. . The oxidation current and the reduction current of the applied water only changed slightly, and the diameter of the Burmese flower only increased by 0.1 cm.

Referring to Table 2 and Figure 5, the same situation also appears in the pocket. Moreover, the increase in height and diameter of the Burmese flower and the pocket in the case of adding the lipotrophic nutrient solution is significantly greater than that of the application of only water, and is proportional to the tendency of the redox current to become larger.

Therefore, according to the change pattern of the redox current measured by cyclic voltammetry, the absorption of the applied fertilizer by the plant 4 can be estimated, and the fertilizer formula can be adjusted immediately for the absorption state of the plant 4 or The application method and the like to effectively promote the growth of the plant 4 can greatly shorten the study time, so it is confirmed that the method can be applied to the immediate monitoring of the fertilizer absorption of the plant 4.

In summary, by inserting the electrode 3 on the plant 4 and applying a liquid fertilizer, the method of performing a redox potential scan can quickly confirm the application of the plant 4 by the change of the redox current. The absorption of nutrients in liquid fertilizers can significantly shorten the time course of research on fertilizer components, and therefore, the object of the present invention can be achieved.

The above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto, that is, the simple equivalent changes and modifications made by the patent application scope and patent specification content of the present invention, All remain within the scope of the invention patent.

Claims (10)

A method for monitoring the absorbance of a plant for application of fertilizer comprises the steps of: (A) inserting two needle-shaped electrodes into a stem of a plant and a leaf vein; (B) a culture medium surrounding the root of the plant Applying a liquid fertilizer; and (C) performing a redox potential scan on the plant via the electrodes, and obtaining a redox current data. The method for monitoring the absorbance of a fertilizer applied to a plant as described in claim 1, wherein the step (C) is to start the redox potential scan after the application of the liquid fertilizer for a while. A method for monitoring the absorbance of a fertilizer applied to a plant as described in claim 2, wherein the step (C) is to start the redox potential scan five minutes after the application of the liquid fertilizer. The method for monitoring the absorbance of a fertilizer applied to a plant according to claim 1, wherein the distance between the two electrodes of the step (A) inserted on the plant is 10 cm or more. A method for increasing the absorbance of a plant for application of the fertilizer by illumination according to claim 1, wherein the oxidation-reduction potential of the step (C) has a scanning range of -2.5 V to 2.5 V. A method for monitoring the absorbance of a plant for the applied fertilizer comprises the steps of: (A) inserting two needle-shaped electrodes into the stem of a plant and a liquid culture medium for cultivating the plant; (B) applying a liquid fertilizer to the liquid culture medium around the root of the plant; and (C) performing a redox potential scan on the plant via the electrodes, and obtaining a Redox current data. A method of monitoring the absorbance of a fertilizer applied to a plant as described in claim 6, wherein the step (C) is to start the redox potential scan after the application of the liquid fertilizer for a period of time. A method for monitoring the absorbance of a fertilizer applied to a plant as described in claim 7, wherein the step (C) is to start the redox potential scan five minutes after the application of the liquid fertilizer. The method for monitoring the absorbance of a fertilizer applied to a plant according to claim 6, wherein the distance between the two electrodes of the step (A) inserted on the plant is 10 cm or more. A method for increasing the absorbance of a plant for application of the fertilizer by illumination according to claim 6, wherein the oxidation-reduction potential of the step (C) has a scanning range of -2.5 V to 2.5 V.