TW201024278A - Uses of thaxtomin and thaxtomin compositions as herbicides - Google Patents

Abstract

There is a need for a selective, low-risk herbicide that can be used to control weeds in cereal cultures and turf. The present invention discloses that a bacterial secondary metabolite, thaxtomin and optionally another herbicide is an effective herbicide on broadleaved, sedge and grass weeds. Thaxtomin A and structurally similar compounds can be used as natural herbicides to control the germination and growth of weeds in cereal, turf grass, Timothy grass and pasture grass cultures with no phytotoxicity to these crops. As a natural, non-toxic compound, thaxtomin can be used as a safe alternative for weed control in both conventional and organic farming and gardening systems.

Description

201024278 VI. Description of the Invention: [Technical Field] The present invention relates to a composition and method for controlling the germination and growth of broad-leaved trees, sedges and weeds, which contains saxtamine (thaxt〇min, a plant toxin) , is a cyclic peptide Streptomyces (Sp) active ingredient. [Prior Art] Natural products are made of microorganisms, plants, and other organisms. Microbial natural products provide a rich source of chemical ingredients, and the use of medicinal natural products has a long history. However, secondary metabolites made from microorganisms can also be successfully used in agriculture to control weeds and pests. Saxedamine (thaxtomin, a phytotoxin) (4-nitroindol-3-yl-containing 2,5-monooxazinidine) is a plant pathogen of the genus Bacillus (Gago plus Sp) The second edition of the plant toxin product, which is a mixture of mold (β. scabies) and β. acidiscabies, which causes horses (9)/(10) veins/w&myww) (King, Lawrenceetal) 1992) Pests and diseases. Toxins are also produced in diseased tissues, which can be cultured in vitro on high quality oatmeal medium (L〇ria, Bukhalid et al. 1995; Beausdjour, Goyer et al. 1999). King et al. (King, Lawrence et al. 2001) pointed out that all of the plant pathogens, key mold, produce one or more herbicide-active thaxtomin (a plant toxin) component. Hiltunen et al. (Hiltunen, Laakso et al. 2006) compared the purification of four sax-tomin (thaxtomin, a phytotoxin) component from the culture broth by Streptomyces faecalis (S.) and IwWcfecW (including Saxedamine A (a phytotoxin), a saxedamine (thaxtomin, a phytotoxin) isomer, a sactomin b (thaxtomin B, a phytotoxin) (thaxtomin B, a plant toxin) And Sacradamine D (thaxtomin D, a phytotoxin)), and showed that all four components, in the in vitro propagation of potato, induced similar inhibition of germination growth, root growth (10-200 ppb) and necrosis Symptoms (200-1000 ppb). In addition, in the mixed ingredients of thaxtomin (a phytotoxin), it showed additive effect 201024278, but no synergy (Hiltunen, Laakso et al. 2006) According to the study of 〇1^(〇1^^奶〇11^1.2003), SacDomin is added to the 〇111 to A, a phytotoxin) (Fig. i) and Sac Damine D (thaxtominD, a plant). Toxin) has significant activity before and after use of non-systemic herbicides 'Of less than 1 uM and the concentration of Sakeduomin A (thaxtomin A, a plant toxin)' clothing and contribute to cell necrosis chamber, and inhibits monocotyledonous and dicotyledonous seedling length J (Healy, Wach et al, 2000). Sachdogin (thaxt〇min, a plant toxin) has been evaluated by Dow Agro Sciences as an active herbicide but lacks a systemic effect (King, Lawrence et al. 2001). In the ankle ring of the Diketopipemzine L, L-structure, the nitro group appears to provide the minimum required phytotoxicity. The nitro position in the anthracene ring is very local, and the phenyl group of alanine plays a necessary role in the phytotoxic structure _ Lawrence et al. 1989; King, Lawrence et al. 1992; King; Lawrence et al 2003). The role of herbicides is to disrupt cell wall synthesis (Fry and Loria 2002) to target inhibition of cellulose biosynthesis (King et al., 2001; Duval et al., 2005; Johnson et al. 2007). recent

Kang (Kang, Sem嶋 et al. 2008) studies that use saxedon (a phytotoxin) and saxedamine (a phytotoxin) as an algaecide. Vine used to control the environment in the water [Summary of the invention] ', the invention of a Qianxiangqing ^ (secret, - a variety of lion poison, used as a herbicide before and after 'in the common animal, pasture, Weicao and artificial grass J., the grass ship. The so-called "growth system" is an ecosystem for the growth of grains, pastures, yarrows and artificial turf. For example, "for growing grains, long culture rings may contain scorpions or seeds. Similarly, the "artificial turf growth system" can be an artificial turf growth system, a picking culture environment or a field, grassland or golf course, which includes the growth of human leather and weeds, including but not limited to scales. Tube numbness, pilgrimage to 201024278, Putian ίί: Fen, Western dandelion, Solanum nigrum, Lobelia mallow, Qiaotailtail, dry money, bluegrass, bluegrass, ryegrass, j, '稗草' and Especially the Anhui cuisine - Li, ragweed - anti-branches , H dishes - mediated wild, British public _ - West m, Solanum nigrum and Lin _ ^ containing her min, - female biotoxins) active ingredient. Another goal is that it does not harm grains, pastures, sedges, and humans: dry-skin growth and environmentally sound weeding methods. Inscriptions 生长 or human turf and grain growth systems. In particular, in the present invention, at least one of the components of the present invention, which may be further contained in the carrier and/or diluent embodiment, is a solution. One with 一 containing a _ thing #素诚份 can be dissolved in the base = release = alcohol 'or fat such as the network, methyl

^Z::Z ΙΙΙΤΛ%ΙΙι^ ϊ 除 = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = = 201024278 Medium: The composition of the fresh growth system cap can be; I; «t ^ί': 5 〇❹ = ΙΓ 3 length 4 髫 including but not limited to corn, wheat, t 1111^ growth system or j'ί Cereal roots: brome, ryegrass, bentgrass, knots: ii ^; tt., a phytotoxin) or salt, effective regulation of inhibition and / or growth of Timothy. Especially provided by the present invention The method, in addition to the sensitive (thaxt〇min, a phytotoxin), can provide at least a second type of herbicide (201024278) mm and a second herbicide component, which can be used for cereals, artificial turf, grazing and / or the growth system of the grass. [Embodiment] In the case where the relevant value is provided, unless otherwise stated, the interference unit value clock is 1/10 of the low limit value, and the interference limit is between the lower limit and the upper limit value; Read or interfere with the value. These smaller fine lower and upper limits can be individually listed in smaller ranges, with the exception of certain excluded values. Mark: The scope includes - two limits, the range of which excludes any one or two interference intervals that are within the relevant range. Unless otherwise indicated, the technical and scientific terms used herein have the same meaning as the ordinary terms used in the field of the invention. Although other methods and materials similar or equivalent to the present invention can also be used in the application and testing of the present invention, the most important thing to note in this document is the "one" used in the content of this document and the proposed patent statement. Or the meaning of "a", unless otherwise stated, may mean singular or plural. The saxedamine (thaxtomin, a phytotoxin) used in the present invention can be obtained from the following actinomycete culture Streptomyces sp. (S. foot 6 (four) - ATCC 49173, S. acz Vfcca Mes - ATCC 49003 And BL37-EQ-〇l〇- or purchased from the market. The saxedamine (thaxtomin, a phytotoxin) used in the present invention includes, but is not limited to, containing cyclo-(L-4-nitrotryptophyl-L-phenylalanyl) a cyclic dipeptide material of a substance. In a particular embodiment, a suitable diketopiperazine group can be N-methylated' and comprises a homologous element comprising a strepalanyl a and a cyclic carbyl group. The chemical composition of the example contains: ' 201024278

R2 R5 0 r4 wherein R! is methyl or H, & is hydroxy or h, R3 is fluorenyl or ’ ' R4 is hydroxy or hydrazine, Rs hydroxy or HH, and Rg is a mixture of hydroxy or η and the like. In accordance with the present invention, suitable unrestricted examples of thactomin (a phytotoxin) oxime component include, but are not limited to, the use of saxedin A (a phytotoxin), sacdo Min (a), thaxtomin A Isomers, viasacsin A (thaxtomin A, a phytotoxin) and des_N_decyl sacredamine C and any derivatives of such substances (cf. Figure 1). The ingredients of the present invention can be sprayed on plants or soils. Specifically, in the case of such cases, the wounds can be dusty, coarse powder, small granules, granules, wet powder, emulsifiable concentrate, liquid preparation, suspending agent, water. Shape or oil degrades the particulate suspended matter. The ingredients of the present invention comprise a carrier and/or a diluent, and the carrier herein is an inert, organic or inorganic material which is mixed with an active ingredient or formulation to aid in the handling, storage, transport and disposal of plants and the subject matter. / or deal with. The diluent or carrier component before and after the use of the herbicide includes, but is not limited to, a liquid, a powder, an alcohol, a mineral oil, and a glycerin component.

The composition of the present invention contains at least two herbicide components, one of which is thaxtomin (a phytotoxin) as described above. As shown in the specific examples, thactomin (a phytotoxin) is dosed from 〇〇1 to about 5.0 mg/mL. Another herbicide is a bioherbicidal and/or chemical herbicide ingredient. The biological herbicide is selected from the group consisting of clove, cinnamon, transcript, orange oil, orange peel oil, toxin, comexistin (a phytotoxin), AAL 201024278 toxin ^AAL-toxin 'a phytotoxin), Ingredients of fibrinone, scutellarin, rice huskone B, scorpion scorpion, ascole toxin (ascaulat〇xin, a phytotoxin) and eskeletinxin (a phytotoxin) glycoside . In a specific embodiment, the ingredient may contain a saturin, a phytotoxin, a citronella oil, a surfactant, and/or a vegetable oil formulation. In yet another embodiment, the composition comprises saxtomin (a phytotoxin), a ruthenium salt, and/or a vegetable oil. The content of the biological herbicide such as citronella oil or sulphate base is about mg/mL to 5 〇 mg/mL, and the optimum ingredient content is about 0.5 mg/mL to 10 mg/mL. Chemical herbicides may be selected from the group consisting of insecticides and salts, wheat straw and salt, topramezone (an insecticide), and tembrit trione (a herbicide). ), S-Metolachlor, Atrazine, mesotrione, fluorine gas, 2,4-dichlorophenoxyacetic acid, tobacco Nicosulfuron, thifensulfuron-methyl, asulam, metaribuzin, diclofop-methyl, fluazif〇p, fine σ 坐 禾 禾 oxa oxa ( fenoxaprop-p-ethyi), sylvain (asulam), oxyfluorfen, rimsulfuron (rimsulfuron), 2 曱 4 chloropropionic acid (mecoprop), and dichloroquine Acid (quinclorac), thiobencarb, clomazone, cyhalofop, propanil, bensulfuron-methyl, penoxsulam, green grass (triclopyr), taste grass smoke (丨11137技1^口 711), chlorine '» than 哺 隆 (11&1〇3111^11:011-1116111>4), pendimethalin, shuangcao ( Bispyrib Ac-sodium), carfentrazone ethyl, s〇dium bentazon/sodium acifluorfen and orthosulfamuron 〇 chemical herbicides such as pendimethalin or Clomazone is a formulation of the herbicide before use. The dosage range is from about 5 mg/mL to 15 mg/mL. Chemical herbicide ingredients such as cyhalofop and S-Metolachlor ), bispyribac-sodium, penoxsulam, after use, the content of ingredients from about 1 mg / mL to 40 201024278 mg / mL, in special cases 'about 15 mg / ml to 35 Mg/mL. The ingredients further comprise an adjuvant which is an ethyl oleate, a polyethoxylated alkyl ester and a sulfhydryl group. This ingredient further contains a surfactant for the formulation of emulsification, dispersion, wetting, spreading, integration and differentiation, stabilizing the active ingredient and improving fluidity and inhibiting rusting. The choice of emulsifier and dispersant, such as nonionic, anionic, amphoteric and cationic dispersants and emulsifiers, the amount of ingredients used will depend on the characteristics of the ingredients, and the dosage of the ingredients will contribute to the dispersion characteristics of the herbicides of the present invention. After use, the ingredients used include montmorillonite, attapulgite and similar expansive soils and thickeners such as xanthan gum, gum arabic and other polysaccharide thickeners and dispersion stabilizers. For example, a nonionic surfactant (e.g., polyoxyethylene (20) monolaurate or polysorbate 60 POE (20) sorbitan monostearate, ethyl alcohol monostearate). The difference in clay concentration is between 0 and 2.5% of the total amount of the component, the range of the polysaccharide thickener is about 〇_〇.5% w/w of the total component amount, and the composition of the surfactant is about the total component. The amount of 〇 _ 5% w / w. EXAMPLES The ingredients and methods of the present invention are as set forth in the following non-limiting examples. The embodiments provide different specific embodiments, but are not limited to the materials, conditions, weight ratios, process parameters, and related inventions herein. Example 1 In a greenhouse treatment study, a 6-inch corn plant (Zea may var. Sunglow) was sprayed with an increasing concentration of saxedamine A (thaxt〇min A, a plant toxin), mixed In 4% ethanol, 〇〇 2 〇 / 〇 polysorbate 6 〇 p 〇 E (20) sorbitol monostearic acid solution. This spray solution contained 〇 125, 〇 25, 〇 5 and 1.0 mg of saxtomin A (a phytotoxin) / mL, and sprinkled over the entire surface of the plant. Each treatment step was repeated 3 times, and the solution concentration control contained 4% 4% ethanol, 0.02 〇/〇 polysorbate 6〇p〇E (2〇) sorbitol monostearic acid as a surface active hydrazine. Before and after treatment, the plants were grown in a greenhouse of artificial light (12-h light/dark cycle) at a temperature of 25 〇c. Plants were evaluated at intervals of 1 week and started on the 7th day after treatment. The final assessment was completed within 3 weeks of treatment. At the time of the evaluation, observations were made on the plants tested using the highest concentration of thaxtomin A (a phytotoxin) without any toxicity. Example 2 In the toxicity test of corn (Zea wi% var. Early Sunglow) and wheat advww var· PR1404), in order to confirm the activity of broadleaf weeds and valerian, it was planted in the same pot, using 3 Corn seeds or 5 wheat seeds were sprayed simultaneously with these test plants. Under the growth light regulation, 'there is less than 3 inches of growth (each 12_h light/dark ϊ ))' /jbl degree is 28 C ' and the sark is made of Streptomyces sphaeroides (S. A solution of sympathetic A (thaxtomin A, a phytotoxin) containing 〇.5 and i.〇mg of saxdamine A (thaxt〇minA, a planting scorpion toxin) (4 ° / 0 ethanol) And 0.2% nonionic surfactant), with 4% ethanol + 0.2% nonionic surfactant but no Sachsin A (thaxt〇minA, one, toxin), the control group used The detection agent was carried out three times, and the plants treated with light growth at 18 ° C were observed on the 7th, 14th and 21st day after treatment, respectively, regarding the toxicity of corn and wheat and valerian. No toxic symptoms were found in grains treated with saxedin A (a phytotoxin) during each observation period. The highest dose of thaxtomin A (a phytotoxin) was used. (1. 〇mg/mL) treated yarrow 'formed with corn and wheat _ control tube growth results. ◎ Example 3 is able to be high In the alfalfa plants, the toxic '贞I〗 of Saccharin A (thaxt〇minA, a phytotoxin) was tested and planted in a 4" x 4" culture potted pot. The plants are grown in the optimal greenhouse conditions by spraying the solution containing 〇5 and [thaxtomin A (a phytotoxin)/mL. When the mouth is sprinkled, the treatment steps are repeated 3 times. And control treatment steps, = substance, 仃 carrier treatment (4% ethanol, 0.02% poly-alcohol vinegar 6 〇 〇Ε 〇Ε (10) Yamanashi monostearic acid). The detection of toxicity is measured at 7-day intervals after treatment. Period 12 201024278 仃The final evaluation was found to be toxic after treatment at any concentration for 3 weeks after treatment. ~ Example 4 Streptomyces solanacearum (Chengcheng (4) (ATCC_49〇〇3) in oat bran

5 5 days (25t>c/ 2(8)), whole cell cultures treated with sacDomin a (^ctomm A, a phytotoxin) were extracted using Sa A (thaXtomin A, a phytotoxin) XAD resin. The dried extract is suspended in 4% ethanol and 0.00 mg/mL in a sub-surfactant. This solution has two different saxedin A axtomin A, a phytotoxin) (〇5 and j concentration components) The following broadleaf weed species were tested: 徽菜-# 冏麻-^ Sunflower-to-no turkey grass--grass-grass, three yellow-and #克旋在-田旋花野芬菜- The public collection - Western dandelion Solanum nigrum L., black - malaria nectar ~ Lobelia mallow a is also used in the following weed species: foxtail - 芑 芑 名 草 ~ ~ ~ ~ ~ ~ ~ ~ ~ 遵 遵 遵 遵 遵 遵 遵 遵 遵 遵Grass - Grassland Swift Ryegrass / Perennial - (, Black Grass) Tall Fescue Gaofeng Table Ansheng 2, LS1100) All plants of 稗萆-稗草 were tested 3 times 4 4" in plastic pots, untreated Control group 13 201024278 The group of plants 'is sprayed with carrier solution components (4% ethanol and 〇〇 2% glyc〇sperse)' and the positive control group of plants, at a rate of 1 fl 〇z / acre Repeat the test. The treated plants were placed in a greenhouse and cultured under a 12 h light/dark cycle. Weekly test evaluation data for broadleaf varieties, as shown in Table j. Table 1 · For different weed species 'Lianke Domin A (tha; xtomin A, a phytotoxin) dry dish disease 4 s. iic / his caW kiss extract control treatment: evaluation level: 0 - not Control, 1 _ 10% control processing, 2 _ 25% control processing, 3-5 〇% control processing, 4_75% control processing, 5 - 100%

Dandelion 0.0 Zhuangyuan 0.0 Microlore 0.0 Ragweed 0.0 Castor 0.0 Convolvulus 0.0 Mustard 0.0 Sunflower 0.0 Mallow 0.0 Herba 0.0 0.0 0.0 0.0 0.0 0.0 2.7 0.0 0.0 2.0 0.0 0.0 1.0 0.0 0.0 1.7 0.0 0.0 1.0 0.0 0.0 3.3 0.0 0.0 1.0 0,0 0.0 1.0 0.0 0.0 3.5 2.3 4.0 2.0 2.2 2.3 2.7 2.0 2.0 2.0 0.5 0.0 1.0 1.0 1.0 2.0 1.0 0.0 1.2 4.0 4.5 3.5 2.0 0.5 1.0 1.0 1.0 1.2 4.0 4.0 4.2 2.0 3.7 2.0 2.3 2.0 2.0 0.5 0.0 1.0 0.3 1.0 0.0 2.8 3.5 1.7 0.5 1.0 1.0 3.0 3.7

S. cerevisiae (S. broth, the most common weed species, in general broadleaf mixed with Saxedamine A (thaxtomin A phytotoxin) at a concentration of 0.5 mg/mL or higher In the artificial turf and grain growth systems of grass varieties (dandelion, mustard and valerian), there is a good effect 201024278 (>50%). Some weed control, such as Solanum nigrum and Huizhou, is not complete, but Sachsin A (a phytotoxin), even at the lowest concentration (〇5 mg/mL), causes poor development of these weeds. In this same study, 〇·5 or 1.0 mg was used. /mL Saxedamine A (thaxtominA, a phytotoxin) concentration treatment, it is seen that there is a counter-effect. Among all the tested weed species, even with a higher Sachsamine A (thaxtominA^ - The concentration of phytotoxin) did not show the toxic effect. Figure 4 shows the treatment effect of different weeds after 1 week of treatment with saxedin A (thaxt〇minA, a phytotoxin) (Downy Brome, Tall Fe_ and Kentucky bluegrass). Example 5 Saxedo A (thaxtomin A, one Plant toxin) and two commercially available herbicides (Bipyribac-sodium blended with Regimem) and lemongrass oil blended with green (GreenMatchEX, herbicide) Sedge and water plants are tested using small pots (1 square ounce). All single product treatments and tank mixes are sprayed at 57 gallons per acre. Percentage of control evaluation reagents are completed after 14 days of treatment. The results are shown in Table 2. The average values of the fields are the same, listed in Table 2, and there is no statistical difference between each other p < 0.05 〇, ❹ According to this result, 25% by weight of lemongrass oil failed to improve Sa The effect of ketomin A (thaxtomin A, a phytotoxin) (〇·25 mg/mL) on sedge, but significantly increased for some aquatic plants (field test) and thousand gold grass (greenhouse test) fruit table 2: ® 克多敏 A (thaxtomin A, a phytotoxin) single drug gradually mixed, double 苎 ft^bispyribac-sodiimO and lemon-like grass oil on the two rice straw treatment period, small chemical yarn early and the water army effect sedge control (%) Aquatic control (%) 95a

Saxedamine (thaxtomin, a plant toxin) 0.25 mg/mL 15 201024278 Saxedamine (thaxtomin A, a plant toxin) 0.5 mg/mL 100a 5d Bispyribac-sodium (12 g/acre) 87.5 a 32.5a bispyribac-sodium % (6g/inch) 47.5c 15c double-grass (bispyribac-sodium) V2 + fk first (thaxtomin Λ, a plant toxin) 0.5 mg/mL 67.5b 25ab Bispyribac-sodium XA + Sacradamine (thaxtomin A, a phytotoxin) 0.25 mg/mL 55bc 7.5c Lemongrass oil 5% 15d 10c Lemongrass oil 2.5% 12.5d 10c Lemongrass oil 1.25% 20d According to the test results, 1.25% of the lemongrass oil failed to improve the effect of saxedamine A (a phytotoxin) (〇25 mg/mL) on sedge, but improved significantly on some aquatic plants (field inspection The effect of _ leaf gold grass. Sachdogin A (thaxt〇min A, a plant toxin) (〇 5 tons) can improve the effect of the als inhibitor Wpyribac sodium, which has a labeled value for sedge and general grasses. Half effect rate. Example 6

A field test of rice was carried out from Streptomyces amyloliquefaciens (&s culture medium), and a culture inspection was carried out using a metal ring pot of 49 square inches of sigh. Sensitive A (thaxtomin A, a phytotoxin) or Sacramento A (thaxt〇min A, 201024278 a plant toxin) mixed with lemongrass oil (mixed to green pair (GreenMatch EX 'herbicide)) or cypress (cyhalofop) (mixed to ClincherCA), after completion of the blending, use a hand-held sprayer to spray 57 gallons per acre. Rice (variety name M209) grows until maturity' then manual harvesting for weed measurement The harvesting results (kg/ha), as well as the relevant detection values of each potted green stalk, sedge and sage, are shown in Table 3. Table 3 · · Effect of ax克多敏 A (thaxtomin A, a kind Phytotoxin) Effect of single agent and mixed with lemon-like grass oil and Cyhalofop on rice harvesting system

UTC ❹ 2 · Sachdogin A (thaxtomin A, a phytotoxin) (i8〇g/acre) 3. Wrestling grass 〇 il 1.25% + Sac Tacmine A (thaxtomin A, a plant toxin) (90 g / Acres) 4. Cyhalofop (-half-labeled value: 52 g/acre) + Saxdormin A (thaxtomin A, a plant toxin) (90 g/inch) + vegetable oil 2.5%

The average value of each block is indicated by the same letter, and there is no statistical difference p < 〇 〇 5. The results showed that the use of sacdomin was 180 g/ying, which significantly reduced the number of sedge, but did not affect the growth or harvest of the ginseng. Use half the ratio (thaxtomin A (a phytotoxin) 90 g/acre) mixed with lemongrass oil, which has a better effect on sedge than cyhai〇fop (using half of the mark) Value 52 g / acre). Sakdogin (9 gram/acre) has a good control of a thousand gold when mixed with half the ratio of cyhalofop - this mixture can significantly improve the yield. Example 7 Cyhalofop (butyl 2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propionate) may also be mixed with ethyl oleate, polyethylene dimer Ester, ethoxylated hydrazine 17 201024278 phenol (2.5% v / v) adjuvant 'and increase the concentration of sac Dominica a espresso, a phytotoxin) (purified from ATCC strain 49003), The concentrations were 〇1 0.2 and 0.4 m g/ml. The concentration of '2-[4-(4-carbo-2-cyclophenoxyphenoxy)propionate before dilution was 29.6% (2.38 lb/gal) and 21.7% (2 lb/gal). The effects of these mixtures on the growth of plantain, sorghum, sedge and sage, are tested in the greenhouse. Similarly, the effects of M104 rice plants on the drug are also related to growth detection, and all The plants were subjected to relevant evaluation tests after 7, 15^21 days of treatment. The results of the 21st day of treatment with Clincher CA were formulated using cyhal〇f〇p, as shown in Table 4 below. Control A Clincher CA (6·5 oz/acre) + 多克多敏 A (thaxtomin Λ a plant toxin) (m g / mL)

The team passed on Nalu, umcfter (JA (29.6 % Cyhal〇f0p) / weight) using half the marked ratio (6.5 〇z/acre), which has good control effect on weeds, but Broad-leaved species or sedges are not effective. 4 tails are combined with aincher ca. Table 4. Sakedomin A (thaxtomin A, a plant ♦ blame) single agent and mixed with cyhalofop, for rice harvesting Dong And the effects of weed control (cyhalofop) and saxedin a (thaxtomin A, a plant poisoning agent) have good control effect on rice weeds in this test. If mixed with Clincher , Saxedo A (thaxt〇minA, a plant has a good effect on weeds. Sac Domin A (th her minA, a planting) Age* - CA riding Yu Lai, the rice music Example 8 Penoxsulam (2-(2,2-difluoroethoxy)_N_(5,8-dimethoxycarbonyl 201024278-based [1,2,4]trisazo [1, 5 (;) gas pyridin-2-71)-6-trifluoromethyl) benzene) mixed with ethyl oleate, polyethylene dialkyl ester and ethoxylated nonyl phenol (2.5 〇 / 0 ν/ν) 'And increase Sackdormin A (thaxt The concentration of ominA, a phytotoxin) (purified from ATCC strain 49003) at a concentration of 0.1, 〇·2 and 〇.4 m g/ml. 2-[4-(4-Cyano-fluorophenoxy)phenoxy ]propionic acid, butyl butyrate or 2_(2,2-difluoroethoxy)-(5,8-dimethoxy[1,2,4]trisazo[1,5C]pyridinoline The concentration of _2_yl)_6_methyl 11 to benzophenone was 29.6% (2.38 lb/gal) and 2U% (2 lb/gal) before dilution. These mixtures were for plantain, sorghum, sedge The effects of growth and the effects of the seeds were measured in the greenhouse. Similarly, the M1〇4 稻 rice varieties were also tested for growth and phytotoxicity, and all plants were treated on days 7, 14 and 21 after treatment. Example 9 Streptomyces faecalis (& fldda & kiss was cultured in oatmeal fermentation broth for 5 days (25 ° C, 200 rpm) 'The whole cell fermentation broth was extracted with resin, and the dried crude extract was extracted. Resuspended in 4% ethanol and 0% nonionic surfactant at a concentration of 10 m/mL. Diluted extract contains 〇2 and 0.4 mg of saxedamine A (thaxtomin A, a plant toxin per ml) ), testing of three kinds of grasses (green fruit) (Amaranthus), small flower sedge (Cygnus) and Thousands of Gold (Thousands of Gold). Other treatments include the use of sputum base at doses of 25 and 5 〇m g/mL, mixed per ml 0.2 mg of sacredin A (thaxt〇minA, two plant toxins) and 2.5 mg of armentine. Each processing step was performed 3 times. The treated plants were placed in a greenhouse and conditioned for 12 h light/dark cycles. On the 25th day after the treatment, the test results are shown in Table 5. ' 口表 5 · Use tt ketomin A (thaxtomin A, a plant 畚 洲 2 and 0.4 m gram / mL) or false 筠 喊 (2.5 or 5.0 m gram such as B) single agent, or mixture ( The effect of 0.2 + 2.5 m g / 11 ^) on the control of overflow culture of broadleaf species, sedge and hybrid grass. Ya morning job processing green fruit ^ system Shakes control Qian Qian control (/ 〇) (%) (%)

UTC 0a

Oa 0a 19 201024278 Sacradamine A (thaxtomin A, a plant toxin) 0.2 mg/mL 5.0b 48.3b 8.3ab Saxedamine A (thaxtomin A, a plant toxin) 0.4 mg/mL 11.7c* 91.7d 10.0b Sa Keduomin 0.2 + False Pavilion 2.5 mg/mL 11.7c* 61.7c 73.3c False pavilion shouting 2.5 mg/mL 0a 8.3a 8〇.〇c False word pavilion 5.0 m/mL 2.5ab 6.7a 92.3 d * stunted There is no statistical difference between each other in the field, with the same alphabet $ sex p < 0.05. ‘Saxdormin A (a plant poison 〇·4 m g/mL provides a good control effect of sedge, the effect is not good. Mixed with fake vestibule reduction, for miscellaneous control

= returning _ effect, she is only in the symmetry of the mesophyll species (green fruit 峨 use all of its J, but the invention has been proposed a number of specific examples, but ♦ detailed description, = even using different equivalents, changes and modifications The scope of the present invention is still covered by the description of the present invention, and the contents of the different references have been proposed, that is, the complete contents in each reference material are cited as explanations. (4) * This article 201024278. References:

Beausejour, J., C. Goyer, et al. (1999). "Production of thaxtomin A by Streptomyces scabies strains in plant extract containing media.n Can J Microbiol 45: 764-768.

Duke, S. 0., S. R. Baerson, et al. (2003). "United States Department of Agriculture-Agricultural Research Service research on natural products for pest management." Pest Manas Sci 59: 708-717.

Duke, S. 0., F. E. Dayan, et al. (2000). "Natural products as sources of herbicides: current status and future trends." Weed O Research 40: 99-111.

Fry, B. A. and R. Loria (2002). 'THAXTOMIN A: Evidence for a plant cell wall target." Physiological and Molecular Plant

Pathology 60: 1-8.

Gerwick, B. C., P. R. Graupner, et al. (2005). Methylidene mevalonates and their use as herbicides. U. p. 7393812: 16.

Healy, F. G., M. J. Wach, et al. (2000). "The txtAB genes of the plant pathogen Streptomyces acidiscabies encode a peptidesynthetase required for phytotoxin THAXTOMIN A ❹ prodcution and pathogenicity." Molecular Microbiology 38: 794-804.

Hiltunen, L. Η., I. Laakso, et al. (2006). "Influence of Thaxtomins in different combinations and concentrations on growth of micropropagated potato shoot cultures." J Agric Food Chem 54: 3372-3379.

Hoagland, R. E. (2001). "Microbial allelochemicals and pathogens as bioherbicidal agents." Weed Technology 15: 835-857.

Kang, Y., S. Semones, et al. (2008). Methods of controlling algae with THAXTOMIN and THAXTOMIN compositions. USA, Novozymes Biologicals, Inc. 21 201024278

King, R. R., C. H. Lawrence, et al. (1992). "Chemistry of phytotoxins associated with Streptomyces scabies^ the causal organism of potato common scab." J. Aerie. Food Chem 40: 834-837.

King, R. R., C. H. Lawrence, et al. (1989). "Isolation and characterization of phytotoxin associated with Streptomyces scabies.” Journal of the Chemical Society. Chemical Communications 13: 849-850.

King, R. R., C. H. Lawrence, et al. (2003). "More chemistry of the thaxtomin phytotoxins." Phytochemistry 64: 1091-1096.

King, R. R., C. H. Lawrence, et al. (2001). "Herbicidal properties o of the THAXTOMIN group of phytotoxins." J Agric Food Chem 49: 2298-2301.

Loria, R., R. A. Bukhalid, et al. (1995). "Differential production of thaxtomins by pathogenic Streptomyces species in vitro " Phytopathology 85: 537-541.

22 201024278 [Simple description of the diagram] Figure 1 shows the structure of saxedin A (a plant toxin). [Main component symbol description]

twenty three

Claims (1)

201024278 VII. Patent application scope: 1.-A method for non-rice grain growth system, artificial turf ίίί's growth system for controlling the growth of broadleaf tree, Sha S weed hair 4, can be fresh and soil shot草草: No = herbicide is saxedamine, the amount of which is sufficient to regulate the sage of the sage, which does not affect the growth of the non-rice grain growth system, and/or the forage growth system, the phase = 2 The method according to item i, wherein the grain contains at least - wheat, wheat, barley, oats, rye, corn, sorghum, sorghum and sorghum, according to the method described in item 1, the artificial turf selection Self-contained, please specialize in the 1 lion, and shoot the singer. Η Wherein the heart is sulfhydryl or Η'% is hydroxyl or h, r3 is indomethacin or H, R5 is through the base or the book, and the heart is the base or H, etc. 5. The method according to claim 1, wherein the weeds are selected from the group consisting of Wei, 苘, 、, 猪草, 反枝1, bribe, wild fen, keupong, sunflower, and mallow, Golden foxtail dry brome, bluegrass, grass early maturing 24 201024278 Remaining No. 2, lsiigg, valerian varieties. From the method of water, biguanide, material and material growth, choose two miscellaneous = Fan Changwei first: a variety of weeding 4 ' * + (four) 7 dependent method, the towel (four) growth system is 9. The method of claim 6, wherein the second herbicidal treatment is a biological herbicide or a chemical herbicide component. 10. The method of claim 6, wherein the second herbicide is a chemical herbicide component selected from the group consisting of difluoropyrrolidin and sputum 叩^ is dicamba sodium (dicamba sodium) ), Topramezone, a killer, tembotrione (a herbicide), S-Metolachlor, Atrazine, A Mesotrione, primisulfbron-mrthyal, 2,4-dichlorophenoxyaeetie acid, nicosuiforon, thifensulfbrnn_methyl, sulforaphane ), metribuzin, didofop-methyl, fluazifop, fine*, fenoxapr〇p_p_ethyi, asuiam, complex Oxyfluorfen), rimsulfliron, mecoprop, quinclorac, thiobencarb, clomazone, cyhalofop ), propanil, bensulfiiron-methyl, penoxsulam, triclopyr, imazethapyr , halosulfUron-methyl, pendimethalin, bispyribac-sodium, carfentrazone ethyl, sodium bentazon/sodium acifluorfen Orthosulfamuron ° 11. The method of claim 6, wherein the second herbicide can be 25 201024278 ί ίίΐ, 'selected from clove, cinnamon, lemon, citrus oil, dial Peel oil, :, (_existin 'a kind of phytotoxin), stealing t-shirts, „ 巧 争 、 、 稻 稻 稻 稻 稻 、 、 、 、 、 、 、 、 、 、 艾 艾 艾 和 和 和 和 和 和 和 和- a plant toxin) component of a glycoside. The first and second methods, except 1Z, as described in claim 6, the herbicide can be used in rotation. The first and second types are used in cereal growth systems, artificial turf, pasture, and/or urinary ❹ ϋ ϋ ϋ ϋ 含有 含有 含有 含有 含有 含有 含有 含有 含有 含有 含有 含有 含有 含有 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少 少/ or the germination of sedge. The herbicide 'containing at least two types of herbicides' and the herbicide of the first type: and the second herbicide may be a biological herbicide or a chemical: 6 as described in the scope of claim 帛ls An ingredient that can be mixed with a nonionic surfactant and/or an organic solvent. For example, the ingredients described in the scope of claim 2, wherein the ingredients may further comprise a nonionic surfactant and/or a fatty alcohol component. 18. In the non-rice, artificial turf, forage and/or Timothy growth systems, at least one of the Sac Dominant herbicides is formulated for the regulation of growth of the leaves, dicots and sedges. Τ 26