TW200422609A - Structure of disposable electro-chemical sensor strip and fabrication method thereof - Google Patents

Abstract

The present invention relates to a structure of disposable electro-chemical sensor strip and fabrication method thereof, whose structure comprises an insulation sheet having at least a through hole, at least a conductive raw material installed in the through hole; a metal film covering the conductive raw material to combine into an electrode, and the electrode has an electrode-acting surface and an electrode-connection surface, so that the electrode-acting surface can be used to proceed the electrode action; and at least a printed conduction strip disposed on the insulation sheet, and having a connection terminal of conductive strip and a signal output terminal, the connection terminal of conductive strip is electrically connected to the electrode connection surface, and the signal output terminal outputs a measurement signal generated by the electrode action.

Description

200422609 V. Description of the invention (l) Technical field to which the invention belongs The present invention is a continuation of the previously applied patent No. 92102 279. The previous patent is an electrode for forming a sensing test piece by plastic injection molding of a plastic conductive composite material, and the present invention continues the patent structure (but the manufacturing method has been changed) to manufacture a metal electrode (Metal Electrode) = Test strips of disposable sensors. The invention is a manufacturing method and structure of a metal electrode (Metal Elec-trode) of a disposable electrochemical sensor strip, in particular a sheet type strip, and is suitable for testing. A component of an analyte in the fluid to be measured, such as the concentration of blood glucose and uric acid in human blood. In general, noble metals are used as electrode materials for electrochemical sensors to obtain a highly stable and reproducible detection quality, which is already a technology in the field of electrochemistry, but it is only the electrodes that really need the characteristics of precious metals on the sensor. Only the surface area, the precious metal materials of other areas are redundant, especially for the throw-away test piece, the precious metals outside the electrode area are wasted. The purpose of the present invention is to provide a method and structure for manufacturing a metal of a low-cost disposable electrochemical test piece. According to the method and structure of the metal electrode provided according to the purpose of the present invention, the precious metal can be greatly used. The amount decreases while ', this. -The metal electrode provided by the objective of the present invention is not only an inert metal electrode that can be used without interference, but also can be used for various metal catalytic electrodes with a straightforward function (not limited to inertness) metal>. Disposable electrodes and sensors made with & can be applied to all kinds of electrical circuits.

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Detection electrodes, biosensors, fluid biochemical composition sensors (such as test strips for sewage, pesticide content, heavy metal components, etc.), test strips for various home medical applications (such as blood glucose, uric acid, cholesterol, etc. test strips) . ^ The principle of the prior art Electrochemical Sensor has been maturely applied to the detection of biochemical components of various fluids for decades, and it is used for electrochemical detection in a laboratory or professional inspection room. , = Different inspection function requirements can be differently equipped. Please refer to the first figure. The most basic structure of an electrochemical detection device 10 is shown in the following table: 1. A container holding a fluid 11 to be measured 11 and a measurement area 11 1 3. Pre 2 · —Reagent 14 is used to produce a chemical interaction with the analyte u in the fluid to be measured, and to generate an output parameter of the electrical parameter. The output value of the electrical parameter is related to the fluid to be measured. The analyte is biochemically related. For example, the fluid to be measured u is human blood, and the analyte is ice of blood glucose =, then the chemical reagent 14 is basically a glucose-oxygen complex. T. The other 3. 2 to 2 test electrodes, such as the number 5 refers to a pair of electrodes (Counter Electrode), 16 is a working electrode (ff〇rking tr〇d6), 17 is a reference electrode (Reference Electr〇de ) For: a working voltage required for a chemical reaction is connected from the -meter 18 to the chemical reactor valley device 12, and the electrical property of the analyte of the fluid to be measured after an electrochemical action The parameter output signal is electrically connected to the numerical analysis and display. i π 1 δ #

I) 200422609

+1 electrochemical meter 18 is used to provide the working voltage (or current) required for the electrochemical action, and measure the output signal current (or voltage) of the electrical parameter after the electrochemical action to make a Record and display the numerical analysis and test data. The test electrodes 15, 16, and 17 can use only two electrodes, which are a counter electrode (Working Electrode) and a working electrode (Working Electrode). Or three electrodes can be used. In addition to the working electrode and the counter electrode, the other is the reference electrode (Reference Electrode). Or you can add more than a fourth Detecting Electrode, which can be configured with different numbers of electrodes due to different electrochemical needs. In addition to the difference in the number of electrodes, due to the different electrode functions (such as the difference between counter electrode and working electrode) and the different chemical reaction components of the sensor, the selection of the material of each electrode varies greatly. The electrode materials commonly used in the laboratory are as follows, where Counter Electrode 5 is only required to be conductive, but the lower the electrical resistance, the better, such as copper rod, silver rod, nickel rod, graphite rod, or The conductive film electrode formed by printing with carbon glue or silver glue, of course, precious metals such as gold and platinum can also be used as the counter electrode material, but the material cost is high. Among them, the material of the reference electrode 17 (Reference Electrode) is most commonly a silver ^ modified silver electrode (Modified Eiectrode) 171. Because the electrochemical working potential of silver gas is very stable, it is widely used as a reference electrode. Among them, the selection of electrode materials for the working electrode 16 (WorkinS Electrode) is more complicated. There are two major directions. The first type is the modified working electrode (Eiectron-Transfer Mediator) using an electron transfer medium.

200422609 V. Description of Invention (4)

Modified Working Electrode), the second type is Metal Catalyzed Electrode. It is immobilized on the electrodes of the first type of electron transfer media—a layer of chemical reagent. The chemical reagent contains an enzyme (such as glucose oxidation enzyme Glu-cose oxidase) and a redox reaction electronic media (Red 〇xMe-di at or, such as those widely used in blood glucose test strips, is one of the secondary blood salts (potassium ferricyanide). After a chemical reaction between an enzyme and a test analyte, a new chemical component is derived (or consumed) ( If H 2 〇 2 is generated, then again: use the electronic medium and the electrons released in the redox process of H202 to generate an electrical signal, and output its electrical measurement parameters through the electrode. The purpose of the working electrode is only for conducting function and not for chemical catalysis function. However, the electrode material used must not produce any self-chemical interaction with the fluid to be measured or chemical reagents and interfere with the measurement accuracy. The electrode material without chemical interference needs to be an inert conductive material, generally-noble metal (such as gold, platinum, palladium, rhodium and other metals) or carbon-containing electrodes (such as carbon film printed electrodes Car—- bon Base Screen Printing Electrode, Graphite

Bar). Because carbon and inert metals do not react chemically at low temperatures, no chemical interference occurs. Because of the high cost of metal, carbon-based electrodes are generally used as the working electrode of the Electron-Transfer Mediator Modi-fied Working Electrode-as for the first type of metal catalytic electrode (Metai-cataiyze ( j Electrode), the working electrode material selected will be

Page 7 200422609 V. Description of the invention (5) The analyte or its derivative directly generates electrification or has a single-selective function on the analyte. 2 It has a direct catalytic effect and then adds an electron transfer medium (Mediat〇 彳 is not required Electrode materials acting as chemical reagents generally contain # Γ. As long as the electrode with direct catalysis type is a metal electrode with a chemical composition, the first and the second are conductive conductive thunders with thermochemical interference, but this type has a direct catalysis effect. g = Analyte reagents must have matching materials with each other in order to have catalysts and chemicals. Different metal electrode materials must be used. The materials of these different analytical metals such as copper, a, zinc, and m poles are not limited to inert materials. (For example, the rh rhodium electrode has excellent and excellent direct contact with H2O2, and the electrode used for electrochemical detection on gold, etc., can contain metal electrodes in the first type, and each electrode can be used in the second type. Or metal electrode. 禋 Metal electrode is not limited to the two types of metal electrodes used in traditional manufacturing methods, the material and manufacturing costs are two, especially the cost of inert precious metal electrodes is wide. Although it has good quality stability, it still cannot become the mainstream of medical testing. The most used amount of electrochemical sensors today is the disposable testing of blood glucose, uric acid, cholesterol and other medical applications at home. Sheet, most of which belong to the aforementioned first category are modified working electrodes (Electron-Transfer Mediatai ·,

Modified Working Electrode), so the electrode can be printed on the electrode with a thin film to obtain a lower cost. Such a typical embodiment is, for example, US Patent No. 985, 116. ,, brother ’As shown in the second figure is US Patent No. 5,99 7,8 1 7

200422609 < f

The product has two metal strips of the same size and both covered with <C 〇ating> a layer of precious metal palladium <Pal ladium> 2 0 1 and 2 0 2 are glued to the insulating sheet 2 0 3 as the metal of the sensor Electrodes, working electrode 204 <Working Electrode> of the sensor, counter electrode 2 05 <Counter Electrode>, electrode lead wires 20 6 and 2 07 <Lead>, signal output terminals 2 08 and 209 <Output Terminal>, etc. All are made of the same palladium metal strip. The consumables of the precious metal handle are relatively large. However, those who really need to use the precious metal palladium material characteristics are the working electrode 204 and the counter electrode 20 5. The rest only need to be conductive. No need for the properties of the precious metal palladium. The third picture is the European Patent No. EP1, 0 98, 00, which is another manufacturing method of metal electrodes. It has plastic injection molding of an insulating sheet 3 (n, which has a recess <Recess> and a partition on the insulating sheet. The electrode pattern <pattern> 302 surrounded by the island <Island> 306 and the electrode lead wires <Lead> 30 3 and the positions of the output terminals 304 and 30 5 are deposited using a metal deposition method <Metallic Deposits &gt; Due to the deposited metal layer on the insulation sheet, the entire surface of the insulation sheet will be covered, and the metal layer on the separation island needs to be processed and removed again, leaving only the metal electrode pattern, the covered lead wire and the output end. The processing cost is high, and it is only suitable for all electrodes made of the same metal. Σ is the reason, in order to reduce the production cost of the metal electrode of the disposable sensor test piece, in order to overcome the problem of wasting precious metal materials, After experiments, tests, and researches, we finally obtained a structure and manufacturing method of disposable electrochemical sensing test strips, in addition to effectively solving the waste metal of the previous metal electrodes. Disadvantage outer material, also such various sensors on gold

Page 9 9 200422609 V. Description of the invention (7) The metal electrode can be stripped in advance in different iron particles such as 1 J and can be modified in advance (Modify μ * Rize n edge sheet) 'It can be shortened—the invention has a metal electrode sense test strip. The main purpose of the present invention is to use—the winter phoenix. ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, π, a metal film, is formed on a conductive material to reduce the precious metal of the metal electrode Decorate Mingguang again: The purpose is to make a large number of metal electrodes in advance-Modlfy the electrode and then install the metal electrode in a through hole of an insulating sheet to greatly shorten the production Between the discardable test piece. This, and another purpose is to use a metal electrode installed in the insulation sheet = a through hole, the area of the through hole is the effective surface area of the electrode, which can be obtained at 2 $ production An accurate and stable electrode area. Because the size of the sensor's measuring side is proportional to the electrode area, the present invention can make the electrochemical formula = reproducibility of the measurement signal (Repr. Yet another object is to use the --- tenon on an insulating sheet to secure the insulating sheet on a measuring instrument in a radial direction. The present invention is a disposable electrochemical sensor strip Structure and manufacturing method thereof, the structure includes an insulating sheet having at least one through hole and at least one conductive material (G Conductive Raw Material), which is installed in the through hole, and a metal film covering the conductive material An electrode is synthesized on the electrode, and the electrode has page 10 0. V. Description of the invention (8) An electrode action surface and an electrode interface surface, an electrode action is performed, and at least one printed conductive electrode action surface is formed on the sheet. And has a conductive bar connection end and -2, which is installed on the insulation bar connection end to be electrically connected to the electrode connection surface, and the signal wheel out end, the conductive output produces a measurement number generated by the function of the electrode. The signal output end is used for the better, and the conductive metal sheet of the sensing test piece is combined into a metal electrode. ', Is a metal, and if the ^ is better, the sensing test piece further includes ## it &amp;于 于 两 乃 又 祜 乃 Chemical reagent, which is set on the% surface, is used to detect an analyte in a fluid sample, and the chemical reagent reacts with a component of the fluid sample to generate a measurement signal. 'f passes the signal output terminal to output the measurement signal. ^ ", 丨' The electrode system of the sensing test piece can form a pole area in the through hole to transmit the measurement signal. ， 车 乂 佳 者 ' The invention can form a through hole on the insulating sheet with a plastic injection device, a punching device or a computer drilling device. Preferably, the present invention can first complete the electrical installation and insulation of the through sheet with the plastic injection device. After passing through the hole, the hole is better. In the present invention, the electrode can be placed in a plastic injection mold // before injecting a plastic material into the plastic injection mold to form the insulation # "'... ... in the through hole ° The mechanical processing device places the electrode into the plastic injection mold piece, and at the same time, the electrode is "encapsulated in the plastic injection" in the through hole. Of course, the sensing test piece may further include an insulating layer To cover The conductive strip brush truck by post good 'material of the metal thin film based sensor strip of gold,

IBM Page 11 200422609 V. Description of the invention (9) Platinum, bond, absolute ruthenium, silver, steel, M, bucket. For the better, the tape of the impression / test piece, iron, or aluminum. It is a conductive material containing carbon or-conductive: cover ::: quality is-conductive gold. Of course: Jinlier plastic material of sensory test piece. Dip plating, metal deposition or spraying metal paint to use a plating, one on top, and the plating device contains a plating solution, which covers the conductive material to form the metal film. ^ The liquid has a metal ion (of course, the conductive element of the sensing test piece (or a ring)), and has a thickness of _0.2 to 3. The heart is considered to be a circle, a moment. The thickness is preferably 0.5mm. The metal of the sensing test sheet is printed with a metal paste device to cover the conductive :: 7 thin metal sheet, and from another viewpoint that can be implemented: And electrochemical test strips, which include a unique H-type discarded through hole, at least one conductive material, which is installed on the +1 by /, has at least one film, and covers the conductive material. Medium and a metal

A use surface and a signal output terminal: 俾 ;; use; electricity; J :: centroid. The signal output terminal is used to output the electrode action. The sensing test piece further includes a thin metal strip, which is installed in the ", The edge of the cymbal sheet" and the metal sheet has an electrode connector L to connect one of the riveted bumps of the electrode. From another point of view, the present invention is a disposable electrochemical sensing test strip, which includes an insulating sheet having at least one recess, page 12 200422609 I. Poor description (10) At least one metal electrode, which The metal electrode is installed in the recess, and the metal electrode has an electrode action surface and a signal output terminal. The electrode action surface is used to perform an electrode action, and the signal output terminal is used to output the electrode action to generate a measurement signal. Of course, the sensing strip may further include a conductive strip, and the conductive strip is integrally formed with the metal electrode to be placed in the recess of the insulating sheet. Of course, the sensing strip may further include a modified layer which is cured Talk on the metal electrode to form a modified electrode.

The electrode function is good for car lovers. The 4 sheet system includes a pair of electrodes, a working electrode, and each of which is provided on the insulating sheet. * Π — * 贝 孑 L Preferably, the sheet system includes a pair of electrodes and A metal electrode of a working electrode is respectively disposed in two through holes of the insulating sheet to perform the function of the electrode. The reference electrode of the metal electrode is used to perform the electrode function

There is &quot; IT-shaped wear

Of course, the working electrode system of the mysterious test piece can be J to be installed on one of the "U &quot; shaped recesses of the insulating sheet. Of course, the test piece system can include a pair of electrodes and a pair of electrodes. A printed electrode is formed by printing a conductive paste on the insulating sheet, and the working electrode is a metal electrode installed in the through hole of the insulating thin film. Of course, the sense test The sheet system can include a pair of electrodes, a pair of electrodes, and a working electrode, and the pair of electrodes and the reference &quot; can be printed on the insulating sheet by a printed two ° ρ brush-conductive 1 刎, and the Working electrode: page 13 200422609 V. Description of the invention (U) Installed in the insulation Of course, if the film and the metal are in accordance with another electrochemical sensing hole, at least one gold has an electrode as the through hole on the sheet The sensing test piece in the middle can further include the electrode as a whole. From an application point of view, it includes an insulated metal electrode. A metal thin film, and the metal is a thin insulating strip conductive strip used to transfer electricity to the electrochemical hole. The surface with the function of the electrode is connected to the gold electrode pad, and the electric connector is connected to the electrode, which is from another type of sensing and at least the test piece, which belongs to the electrode surface and function, and has an electrical connection electrode function. A phase test piece, which is installed on the electrode connection surface of the hair sheet, through holes, and at least one printed conductive and conductive strip connection end, and a metal electrical layer that generates a quantity of test piece to the electrode connection. Let ’s see that it includes a metal electrode, an electrode active surface, and a measuring electrode that performs an electrode. The insulation signal is installed. Enter the k number and the use number. The signal is for the integral molding surface. The pole is a "thin hair sheet" at the exit end, and the exit end is a retreat type which has at least a consistency, and the metal electrode uses the electrode to act as noodles, which is installed in the A signal output terminal, the signal output terminal is a copper electrode. It is a disposable type that has at least one through hole, and the metal puppet uses the electrode to output the electrode conductive strip. And the conductive strip is placed in a recess of the insulating sheet. Of course, the sensing test strip can include a pair of electrodes, a reference electrode, a working electrode, a fluid test recess and a cover layer, and the pair of electrodes and The reference electrodes are formed by printing a conductive paste on the cover layer.

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V. Invention Description G2)

A printed electrode, and the working electrode is a metal electrode in the hole. Of course, the insulating sheet of the sensing sheet can accommodate three electrodes, and the insulating sheet has a tenon and a measuring instrument, and the tenon is fixed to the measuring sheet. The best one is that each of the electrodes of the sensing test piece has a signal output contact point for connecting a signal connection point of the measuring instrument. If it is viewed from another technical type, the present invention is a method for manufacturing a disposable electrochemical sensing test piece. The method includes the following steps to provide an insulating f-piece: it has at least two recesses, and prepares a conductive material group, which has Evening-first-and-second conductive material, 1 little modification completes the first conductive material to form a modified electrode, and the modified electrode and the second electrical material are placed in the two recesses to form the disposable type Chemosensory test piece. Preferably, the sensing test piece further includes a plating process, and a metal thin film is formed on the first conductive material to obtain the modified electrode. Che Fu Jia Zhe ’the sensing test piece further includes a curing process of a chemical reagent to obtain an enzyme electrode. The car tester ’s sense test piece further includes a printing program for printing at least one conductive strip to form at least one signal output terminal, and electrically connecting the output signal of the electrode to the signal output terminal. The invention is a manufacturing method and structure of a disposable detection thin-film metal electrode, which comprises a non-chemical + interference h-type metal electrode suitable for a first type of electron transfer medium modified electrode and a second type of metal catalytic electrode (not Limited to

There are three through holes to place the sheet in the '-^ slot of the meter

Page 15 200422609 V. Description of the invention (13) Inert metals). There are many abandonment with metal electrodes, the main demand is to obtain better = profit Γ: has not provided a low-cost method of making metal electrodes "π: 1 = metal catalytic electrode, but also because the metal of the present invention = The cost is extremely low, so it can be suitable for the production of reference electrode and counter electrode at the same time. In addition, the electrode structure of the present invention can obtain a very accurate electrode working area. Because the ratio of the measured current of the electrochemical sensor to the working surface of the electrode Therefore, the present invention can greatly improve the measurement accuracy of the electrochemical sensor = degree (Reprodueibility). Dan Jianfsheng Feng Huahua = explanation of the concept 'that can observe the use of discarded electrochemical sensor test The structure and manufacturing method of the sheet are indeed: a thin film: formed on a conductive material to reduce the metal cost of precious metal = amount of use, and has a metal electrode installed in a beaker of an insulating sheet to The 2 colors required for the production of the disposable test piece are greatly shortened, and it is easy to explain that the present invention can be implemented by the following preferred implementations; and the drawings can be better understood. Representative symbols of the main parts: 10 12 14 16 171 Counter electrode reference electrode gauge for fluid measurement area to be measured: Insulation sheet electrochemical detection device holding container 13 Chemical reagent J 5 Working electrode i 7 201, 202: Metal strip silver / vaporized silver modified electrode 18 203 200422609 V. Description of the invention (14) 2 04: Working electrode 2 0 5:: Counter electrode 2 0 6, 207 • Lead wire 2 08, 209 Signal output terminal 301 Insulation sheet 3 02:: Electrode pattern 303 Electrode lead wire 304, 305 Output terminal 306 Divided island 401 Insulation sheet 402 Through hole 403 Conductive material 404 Metal film 405 Conductive strip interface 406 Signal output terminal 407 Insulating layer 408 Electrode interface 409 Electrode active surface 410 Conductive strip / guide line 411 Electrode 412 Electrode area 413 Enzyme electrode 414 Chemical reagent 501 Insulation sheet 502 Through hole 503 Conductive material 504 Metal film 505 Conductive strip interface 506 Signal output terminal 507 Insulating layer 508 Electrode interface 509 Top surface 510 Conductive strip 603 Metal electrical Pole 604 Riveted bump 605 Electrode working surface 606 Metal thin strip 607 Conductor connection hole 608 Signal m Outlet 701 Insulation sheet 702 Through hole 703 Metal piece 704 Electrode working surface 705 Electrode lead wire 706 Lead wire 706 Lead wire 706 Lead wire

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200422609

8 0 2: Electrode 1001 1003 1005 Insulating sheet ring through hole chemical reagent 1 0 0 7: Conductive strip connection im: Insulating sheet 9 0 0: Test container 1 0 0 2: Rectangular through hole 1 0 0 4: Rectangular electrode 1 0 0 6: Ring electrode 1 0 0 8: Signal wheel outlet 1102 1104 1106 1108 1301 1303 1305 1307 1309 1311 1313 1315 1401 1501 1503 1506 1508 1510 1103:: Working electrode 1105:: Counter electrode 1107:: Vaporized silver modified layer 1109 :: Signal wheel output end 1 302 :: Through hole 1 304: ► a. Reference electrode 1 30 6 :: Place recess 1 30 8 :: Channel entry 〇 1310 :: Cover layer 1312 :: Signal output end 1314: : Capillary channel 1316, 1317: Abutting convex ridge 1402 Inserting π 1502 &quot; U "shaped recess 1504 Mating surface through hole 1507 Electrode connecting surface 1509 Conductive strip connecting end 1511 Third printed conductive strip

Through-hole reference electrode Chemical reagent Conductive strip Insulating sheet Working electrode Counter electrode Chemical reagent Convection air hole Conductive strip Conductive material Metal film Measuring instrument Insulating sheet Working surface through-hole Electrode surface First printed conductive strip Working electrode wheel out

Page 18 200422609 V. Description of the invention (16) 1512 Reference electrode 1513 Reference electrode output terminal 1514 Second printed conductive strip 1515 Second electrode terminal 1516 Counter electrode output terminal 1517 Chemical reagent 1518 Silver / silver chloride modified layer 1519 Insulation layer 1520 ， C π-shaped opening 1521 Covering layer 1522 Capillary convection air hole 1523 Flowing into D 1601 Insulation sheet 1602 First side 1603 Second side 1604 First recess 1605 Through hole 1606 First conductive strip 1607 First electrode terminal / working electrode 1608 No. One electrode output terminal 1609 Chemical reagent 1610 Second recessed section 1611 Second recessed section 1612 Third conductive strip 1613 Second electrode terminal / reference electrode 1614 Third signal output terminal 1615 Modified layer 1616 Second conductive strip 1617 Counter electrode 1618 Second signal output End 1619 Insulation layer 1620 ”c” Shape α 1621 Cover layer 1622 Capillary convection air hole 1623 Fluid into D 1701 Insulation sheet 1702 Fluid measuring recess 1703 Test Agent placement recess 1704 through hole 1705 through hole 1706 capillary convection pore 1707 working electrode 1708 chemical reagent 1709 second conductive strip 1710 second electrode terminal 1711: electrode output terminal 1712 silver / silver chloride modified layer

Page 19

4 200422609 V. Description of the invention (17) 1 7 1 3 ·· The third printed conductive strip 1 7 1 5 ·· Reference electrode output terminal 1717: n C π-shaped opening 1 7 1 9: Connection terminal 1 8 0 1: Insulation Sheet 1 803, 1 804, 1 80 5: Through holes 1808, 1809, 1810: Electrode 1 8 1 3: Signal output surface 1 8 1 5: Mesh sheet 1817: Opening 1 8 2 0: Measuring instrument 1 8 2 2: Input signal connection point Embodiment 1 71 4 · Third electrode terminal 1716: Cover layer 1718: First conductive strip 1720 · Output terminal 1 8 0 2: Fluid measuring recess 1 80 6, 1 80 7: Card Strip 1811 electrode active surface 1814 chemical reagent 1816 cover 1818 sheet bottom surface 1821 inserted into the card slot 凊 Refer to the fourth figure (a) ~ (c), it is a single electrode detection sheet 'shows a disposable electrochemical sense of the present invention The structure of the test piece includes an insulating sheet 40 (also referred to as an insulating substrate), which has at least one shell hole 402 'at least one conductive material 403 which is installed in the through hole 402' and a metal film 404 'Cover on the conductive material 403 to synthesize an electrode 411, and the electrode 4 11 has an electrode function 409 and an electrode interface 408 ', using the electrode active surface 409 to perform an electrode function (that is, as an electrode), and at least one printed conductive strip 4 10, which is mounted on the insulating sheet 401, It also has a conductive bar connecting end 405 and a signal output terminal 406. The conductive bar connecting end 405 is electrically connected to the electrode connecting surface 408, and the signal output terminal 406 is used to output a measurement signal generated by the electrode action.

Page 20 200422609 V. Description of the invention (18) The conductive material 4 0 3 of the sensing test piece may be a metal, and a metal electrode 4 1 1 is synthesized with the metal thin film 4 0 4. The sensing test piece further includes a chemical reagent 4 1 4 which is disposed on the electrode active surface 4 0 9 of the metal electrode 41 1 to form an enzyme electrode 4 1 3 for detecting an analyte in a fluid sample. The reagent 4 1 4 reacts with a component of the fluid sample to generate a measurement signal, and outputs the measurement signal through the signal output terminal 4 06. The electrode 411 of the test piece can form an electrode area 412 'in the through hole 402 to transmit the measurement signal. That is, the insulating sheet 4 〇1 has at least one through hole 402. A metal thin film 4 04 is covered on a conductive material 403 to form a metal electrode 411. The metal electrode 411 is placed in the through hole 402 to form a tight hole. In addition, the insulating sheet 402 tightly covers the side of the metal electrode 411 so that only one electrode active surface 409 and electrode connecting surface 408 are exposed, and the electrode active surface 40 g is used to perform an electrode function. The connection of the electric bar is connected to the back of the electrode, and the isolation is to be tested, so as not to affect the film, it can be gold, iron, etc. Combined as 10- printing a conductive strip 4 1 0 on the insulating sheet 4 0 1 A y π end 405 and a signal output end 406, the conductive strip connection end 405 electrode connection surface 408, and the signal output end 406 uses To output one of the measurement signals. An insulating layer 407 is coated on the insulating sheet, and only the signal output terminal 406 of the printed conductive strip 410 is exposed, which is used to detect the noise of any conductive part other than the fluid-electrode active surface 409. The final electrode material of this embodiment is the metal thin film 404. The selection of the material, platinum, rhodium, palladium, ruthenium, silver, copper, nickel, titanium, and conductive material 403 should be beneficial to the metal thin film. , The material can be any conductive metal or any carbon-containing conductive material

Page 21 200422609 V. Description of the invention (19) or plastic material treated with conductive coating. = The method of covering the thin film 404 on the conductive material can be: * plating, metal precipitation method, metal spraying, etc. The metal film 404 is covered after the exclamation hole, and the conductive film is conductive. The conductive film can be placed in the first place ^ 404 ^ # X ^ ^, u〇2, 0 VA, 1! I ΐ ^ ^ ^ ^ ^ A large number of electric shovel μ. For example, the procedure of Dangjiajia is to first place the guide in the same-mining trough: the top two middle. Conductive film 404 is then inserted into through-holes 402 oxygen copper, bronze, phosphor copper, white copper, etched # 80 gold such as two copper, Wu • Po Bao # copper, the copper metal is easy to form each belly 4 (U Road Hair / conductivity is suitable for modifying metal thin on electroplating and other procedures. The material of plastic / ΛΓ403 can be a carbon-containing plastic conductive composite material. The shape of the carbon-conducting conductive material 403 is formed by the lithography method, which contains ίΐϊί with conductivity suitable for Modification of the current plastic plating on electric power and other procedures "is a very mature and well-known technology. 1 You can shoot people in general plastic materials, and you can use 4", ", ...", in plastic type The plastic material Tu Miao I can be coated with a conductive layer to get the current plating of f 404. Generally, the original plastic material is first plated with a layer of nickel for two Γ ΐ = ΐ on the upper metal film 404. Printing The conductive conductive paste material of the conductive strip 41 〇 may be any one of carbon, silver, copper, nickel, aluminum, 3 gold and 3 stainless steel or a mixture of conductive adhesives. The material of the insulating sheet ^ 01 may be a polymer Polyvinyl chloride (PolyVinyl chloride), Polypropylene, Poly Carbonate PC (p〇icarb〇n-ate), PBT (Polybutylene)

I 200422609 Five 'Explanation of invention (20)

Terephthalate), poly (ethylene terephthalate), pET (poly-ethylene terephthalate), poly (phenylene oxide), or propylene butadiene styrene resin Aβ3 (AcTylonitrile Butadiene Stytene) And so on plastic resin. The thickness of the insulating sheet 401 may be from 0.2 mm to 3.0 mm. The conductive material 40 may be circular, rectangular or ring-shaped, and its thickness may be greater than the thickness of the insulating sheet 40 i ^ 〇 · 00mm-0 · 15mm. The metal thin film 404 covering the electrode 411 may be FAW gold, and the metal thin film covering the 411 electrode may be changed to silver, and a silver / chlorine layer may be formed by vaporizing a silver layer. Reference electrode of silver. Also, the metal thin film 404 covering the electrode 411 may be platinum, or the metal thin film 404 covering the electrode 411 may be a germanium metal. The metal film 404 covering the electrode 4m may be a palladium metal, and the metal film 404 covering the electrode 411 is: ruthenium = metal. Of course, the conductive material 403 of the electrode 411 may be a copper metal and the covered metal film 404 is also a copper metal, which means a pure copper metal electrode. Electricity $ i i is the through hole 402 on the insulating sheet 401. The through hole 402 can be formed by a plastic injection device, a forming device or a computer drilling device. If the electrode 411 is placed in the through hole 402 / plastic injection device, the stamping device or the computer is read on the insulating sheet 401, and then a mechanical plus = 411 is placed into the Through holes and form a tight fit. In addition, you can change Bai Zhuang +1 J to "plastic injection dagger" method, put the electrode into a plastic injection mold in advance, and insert a plastic material into the plastic injection mold to form the ^, when the electrode Enclosed in the through hole. Ba Yuan Bodypiece ’Yatong

200422609 V. Description of the invention (21) First: The fifth picture (a)-(b) of Mai read is for a single electrode detection sheet, the edge sheet 501 has at least a through hole 502, a conductive material is placed on A tight fit is formed in the through hole 50 2, and a metal thin film 504 5. 3 is synthesized on the top surface 50 9 into a metal electrode (at this time; electricity: = material: a metal or a carbon-containing plastic conductive composite material), The metal electrode has a y-electrode active surface 504 and an electrode interface 508. The electrode active surface 504 is used to perform a% polar action 'and a conductive strip 5 10 is printed on the insulating sheet 501 and has a conductive property. The strip connection end 505 and a signal output end 506, the conductive strip connection end 505 are electrically connected to the electrode connection surface 508, and the signal output end 506 is used to output a measurement generated by the electrode action Signal, reference numeral 507 refers to an insulating layer. The method of coating the metal thin film 504 on the conductive material 503 may be electroplating, dip plating, metal precipitation method, spray paint or metal printing paste. According to another practical point of view, the printed conductive strip 4 1 0 in the fourth figure can be replaced. Please refer to the sixth figure (a) to (c), which is a single electrode detection sheet. The embodiment in the sixth figure replaces the printed metal strip 4 in the embodiment of the fourth figure with a thin metal strip 606. The thin metal strip 606 has a signal output terminal 608 and a conductive strip connecting hole 607, and the conductive The strip hole 607 is electrically connected to a riveting bump 604 on a metal electrode 603 to output the inside of the electrode, and the mark 5 tiger 6 05 refers to the electrode active surface. The electrode is a pure metal electrode. The electrode may also be a pure iron metal electrode. From another point of view, the conductive material 403 in the fourth figure can be changed to a pure metal conductive material to form a metal electrode, and has a recess to cover the through hole and the metal conductive strip, please Refer to the seventh diagram (a) ~ (b), which is a single electrode detection sheet. The embodiment of the seventh diagram 200422609 V. Description of the invention (22) The integrally formed metal strip 70 3 is used instead of the metal of the sixth embodiment. The thin strip 606 and the metal electrode 603, and the metal strip 703 have an electrode working surface 704, an electrode lead wire 705, and a signal output terminal 706 (this metal piece 703 can be a plastic conductive material injection molding device or a metal stamping device to the electrode working surface 7 0 4. The electrode lead wire 705 and the signal output terminal 706 are formed in a single body, which is different from the printed conductive strip 4 0 in the fourth figure). The insulating sheet 70 1 has a through hole 702 and a recess 70 7 (recess). The metal piece 703 is placed and formed into a tight fit. The procedure of covering a layer of metal film (not shown in the figure) on the metal strip 70 3 can be put into the through hole 702 of the insulating sheet 701 after covering the metal film on the metal strip 703 first. The metal strip 70 3 is inserted into the through hole 70 2 of the insulating sheet 701 before covering the metal film. Please refer to the eighth diagrams (a)-(b), which are schematic diagrams of a modified electrode. The metal electrodes completed according to the fourth to seventh diagrams, the appropriate selection of conductive materials and metal thin film materials can be applied to the working electrode (Working Electrode) or Counter Electrode, and in the electrochemical sensor application, a modified layer 801 can be cured on the electrode 802 through a specific manufacturing process to modify the pure electrode 802 ( Modify) Modified Electrode. For example, a silver electrode coated with a silver metal film 〇2 is immersed in a potassium gas solution by electrochemical action or printing to chemically combine the silver layer with the gas to form a silver / silver chloride reference electrode (Ag / AgCl Reference Electrode). Or immobilized (immobilized, coated) — layer of chemical reagent (

Reagent) 41 4 on the metal electrode 4 π in the fourth figure, the chemical reagent 41 4 series

Contains an enzyme catalyst (Enzyme), an acid test buffer (PH

Page 25 200422609 V. Description of the Invention (23)

Buffer), a surfactant (Surfactant / Surface Active Agent), a redox reaction electron mediator (Re (Jox Mediator)), a hydrophilic polymer (Hydrophilic Ploymer Compound) and other mixed compounds, An enzyme electrode (Enzyme Electrode) 413 is formed. The chemical reagent 41 4 on the enzyme electrode 4 1 3 is used to generate a chemical reaction with an analyte in a fluid to be measured and generate an electrical signal. The electrode 4 π outputs the The electrical measurement signal is sent to an electrochemical analysis instrument 18 (detailed ninth figure) to calculate the concentration of the analyte. The chemical reagent (Reagent) 414 of the enzyme electrode 413 above contains the redox reaction electronic medium Redox Mediator (Electron-Transfer Mediator Modified Working Electrode)

Electrode). As for the enzyme electrode 4i3 as above, if the redox reaction electron mediator (Redox Med-iator) in the chemical reagent (Re-agent) 414 is discarded, and a metal thin film electrode material is matched, it will be able to be A metal catalytic electrode (Metal-Catalyzed Eiectrode), such as a platinum metal catalytic electrode. The enzyme electrode 413 as described above, and the enzyme in the chemical reagent 414 may have a "# lucid oxidase (Glucose 〇xidase)", which is used to test the whole blood of a human body and obtain an analysis result of one of the fluid samples. The result of the analysis is a blood glucose concentration in the human blood. As above = the enzyme in the reagent 414 can be changed to have a urea oxidation = extremely thought to test a human whole blood, and an uric acid in the blood The above enzyme electrode 413 'The enzyme in the chemical reagent 414 can have two solid

Page 26 200422609

The alcohol oxidizing enzyme is used to test the whole blood and cholesterol level of a human body. The analysis of η in the human blood is: Ξ Ξ The ninth figure is an application example in which the three disposable test sheets of the present invention are combined to replace the traditional electrodes in the first figure.

Each conductive material can be modified with a metal film to form a specific electrode, such as a gold film counter electrode (Counter Electrode) 409, a silver film reference electrode 802, and a silver / silver chloride (Ag / AgC1) modified layer 8 〇1 also becomes a reference electrode (Ag / AgCl Reference Electrode) and a platinum thin film electrode modified into an enzyme working electrode (Enzyme Electrode) 413 containing a chemical reagent layer 41 4 three disposable test sheets to replace the first A picture of an application example of a conventional electrode. According to another application point of view, the recess 707 in the seventh figure can be changed to have only through holes without covering the metal conductive strips. Please refer to the tenth figures (a)-(b). The embodiment of the electrode detection sheet, the embodiment of the tenth figure is a rectangular through hole in the insulating sheet 1000 and a circular through hole 1 00 3, a ring counter electrode (Counter Electrode ) The metal electrode 1 0 06 and a rectangular working electrode (working electrode) are placed in the through holes 1003, 1 0 2

Form a tight fit, the two conductive strips connecting ends 1007 are placed under the insulating sheet, each of the two conductive strips has a signal output terminal 1008 and has an electrical connection with the metal electrode 1 0 0 4 1 0 6 The measurement signal applied by the electrode; a chemical reagent 1005 is solidified on the working electrode 1004, and is used for generating a chemical reaction with an analyte in a fluid to be measured and generating an electrical signal through the electrode 1004, 1006, conductive strip 1107, and output terminal 1008, output the electrical

Page 27 1 · 200422609 &gt;# V. Description of the invention (25) The property measurement signal is sent to the electrochemical tester 18. Please refer to the eleventh figures (a)-(b), which are examples of the three-electrode detection sheet of the present invention. An insulating sheet 1101 has three through holes 1102, a pair of electrodes (Counter Electrode) 1105, A working electrode 1103 and a reference electrode 1104 are respectively placed in the through hole 1102 to form a tight fit. Three conductive strips 1 1 0 8 are placed below the insulating sheet 1 1 〇1, and each of them It has a signal output terminal 1109 and is electrically connected to each metal electrode 1103, 1104, 1105. In order to output the measurement signal that the electrode functions, a silver chloride modified layer 丨 7 is placed on the reference electrode 11 On 04, the modified electrode 1104 became an Ag / AgCl Reference Electrode, and a chemical reagent 1106 was cured on the working electrode 11 0 3 'to generate a chemical with an analyte in the fluid to be measured. An electrical signal is generated and reacted, and the electrical measurement signal is output to the electrochemical tester 18 through the electrode 1 103 and the conductive strip output terminal 1109. Please refer to Fig. 12 ', which is an application example of a three-electrode detection sheet according to the present invention. A disposable three-electrode detection sheet completed in the embodiment of Fig. 11 is used instead of the conventional electrode use example of the first drawing. Please refer to the thirteenth figure (a) — η ^, v _ _ _ ,, (c) '疋 is a three-electrode detection thin sheet with capillary flow channels of the present invention g &amp; Chutianxiang ^ β 杳 AU ^ 4 An example of the application of the film is shown in the fourth to twelfth embodiments. It is suitable for the detection of fluid called Θ fluid. The disposable blood glucose test structure is used to adsorb a small amount of a test sheet attached to a pole. If part of the electrode is not covered; the body and the body are completely covered by the entire body of the test k body, which will cause the test. wrong

Page 28 200422609 V. The description of the invention (26) is incorrect. This adsorption structure generally has a capillary channel inlet 1 308, a capillary flow channel 1 3 1 4 and a capillary convection pore 1 3 0 9. Capillary channel opening 1 3 1 4 is usually also a measurement area of the fluid. When the fluid to be measured hits the capillary channel inlet 1 3 0 8 and the capillary channel 1 31 4 uses a capillary phenomenon to automatically adsorb the fluid to the measurement area, it will automatically stop and no longer suck. The embodiments in FIGS. 13 to 18 are all embodiments of a detection sheet suitable for detecting a small amount of fluid, and FIG. 13 is an embodiment of a disposable three-electrode detection sheet for detecting a small amount of fluid. An insulating sheet 1 3 0 1 has a fluid measuring recess 1 31 4 (that is, a capillary flow channel 1 3 1 4), and a bottom face of the fluid measuring recess 1 3 1 4 has a chemical reagent for placing the recess 1 306. A chemical reagent 1 307 is placed and a uniform thickness coating is placed. The bottom surface of the reagent 1307 placement recess 1306 has three through holes 1302. The through holes 1302 are used to place a working electrode 1303 and a pair of electrodes. 1 30 5 and a reference electrode 1 304. The three electrodes 1 303, 1 304, 1 305 form a tight fit in the three through holes 1 302. Each of the electrodes 1303, 1304, and 1305 is covered by a conductive material 1313 and a metal thin film 1315 (see the thirteenth figure (d )), Each forming a metal electrode 1303, 1304, 1305, each electrode 1303, 1304, 1305 each has an electrode active surface and an electrode interface, 俾 use this electrode active surface to perform an electrode function, two conductive bars 1 3 1 1 is placed on the bottom surface of the insulating sheet 丨 3 〇 丨, each conductive strip 1311 has a conductive strip connection end and a signal output end 1312, each connection end of the conductive strip 1311 has an electrical connection with the electrode connection surface, It is thought that the signal output terminal 1312 is used to output an electrode measurement signal; the fluid measurement recess 1 3 1 4 has a fluid inlet 1 3 08 and a capillary convection air hole 3 0 09, one time

Page 29 200422609 V. Description of the invention (27) The tritium layer 1 3 1 0 is placed above the fluid measuring recess 1 3 1 4 to form a complete capillary adsorption structure. Above the insulating sheet 1 3 〇 there are two abutment bumps 1 3 1 6 and 1 3 1 7 to abut the cover layer 1 3 1 0, and the abutment bumps 1316, 1317 are used to isolate the fluid sample from the One of the adhesives on the cover layer 1310. The chemical reagent 1307 is placed on the top surface of the three electrodes 1303, 1304, and 1305, and is used to generate a chemical interaction with an analyte in the fluid to be measured and generate an electrical signal. The magnitude of the electrical signal is the same as that of the analyte. The concentration has a proportional relationship. The electrodes 1 3 0 3, 1 3 0 4, 1 3 0 5 output the electrical signal to the signal output terminal 1312 and connect to the meter 18 to calculate the parameters of the analyte. Each electrode The conductive materials 1315 of 1303, 1304, and 1305 can be any conductive material. The metal thin film 1315 covering the working electrode 1303 may be gold. The metal thin film 1315 covering the reference electrode 1304 may be silver, and after processing a vaporized silver layer, a silver / vaporized silver reference electrode is formed. The metal thin film 1315 covering the pair of electrodes 1305 may be a platinum. The metal thin film 1315 covering the working electrode 1 303 may be changed to a coin metal. The metal thin film 1315 covering the working electrode 1 3 0 3 may be a metal. The metal thin film 1315 covering the working electrode 1 303 may be Yishu metal ★, the conductive material 1313 of the working electrode 1 303 can be a copper metal, and the covered metal film 1315 can also be a copper metal, which means that this electrode can be a copper original material and the covered metal film 1315 each other One piece. 'Please refer to the fourteenth figure, which is a combined application of the embodiment of the detection sheet of the thirteenth to seventeenth figures of the present invention and an electrochemical meter. An electrochemical measuring meter 1 4 0 1 has an insertion port. 1 4 〇2, the insertion port 1 4 0 2 is used to introduce a sheet-type electrochemical detection sheet 1301, the fluid to be measured is entered from the capillary channel

Page 30 200422609 V. Description of the invention (28) Inhaled into the sheet. The measuring instrument 丨 4 〇 1 is used to provide a working voltage required for the electrochemical action to each electrode, and receive the measurement signal output from the electrode, after calculating the measurement signal to display an information. Please refer to the fifth figure (a)-(c), which is an application example of another three-electrode detection sheet with a capillary flow channel according to the present invention. This embodiment combines a metal electrode of the present invention with a working electrode 1 50. 5. Use the metal thin film of the metal electrode to obtain good working electrode performance. In addition, the present invention can form a working electrode 1506 in the through hole 1 50 3 to obtain a very accurate working area. Quality reproducibility. For the counter electrode 丨 5 丨 5 and the reference electrode 1512, the electrode material and the area of the electrode do not need to be as precise as the working electrode 1505, so the counter electrode 1515 and the reference electrode 1512 can be produced by conventional printed electrodes to reduce costs. An insulating sheet 1501 has an electrode active surface through-hole 1503 and an electrode interface surface through-hole 1 504, and the two through-holes are connected at the bottom 1 502 to form an n U &quot; shaped recessed portion 502 ,; A "U," shaped electrode sheet 1 505 (that is, having a ·, U ,, shaped cross section) is placed in the "U" shaped recess 1 5 0 2 and forms a tight fit, "υπ shaped electrode sheet 1505 It has an electrode active surface 1506 and an electrode connection surface 1507. Using the electrode active surface 1 506 to perform a working electrode function, a first printed conductive strip 1 5 0 8 is printed on the insulating sheet 1 5 0 1 and covered with The first printed conductive strip 1 5 0 8 on the electrode connection surface 1 500 has a conductive strip connection end 1 509 and a working electrode output end 51 1. The conductive strip connection end 1509 and the electrode connection surface 1507 are electrically charged. Connect, and output the electrode measurement signal to the working electrode output terminal 1510. A second printed conductive strip 1 5 1 4 and a third printed conductive strip 1 5 11 and the first

Page 31 200422609

A printed conductive strip 1 5 0 8 is simultaneously printed on the insulating sheet 丨 5 〇 丨, a second printed conductive strip 1514 has a pair of electrode output terminals 1516 and a second electrode terminal 1515 to be a pair of electrodes 1 5 1 5 and a third printing The conductive strip 1 5 has a reference electrode output terminal 1513 and a third electrode terminal 1512, which are regarded as a reference electrode 1512. The reference electrode 1512 has been modified by a silver / silver gas modified layer 1518 to become silver chloride. Reference electrode; an insulating layer 1519 has a "C" shape opening 1520 and covers the three conductive strips 1 5 08, 1 5 1 1, 1514. Only the electrode active surface 1506 and the electrode output end 1510 are exposed. A cover layer 1521 has a capillary convection air hole 1 5 2 2 and covers the n C11-shaped opening 1 5 2 0 to form a complete capillary adsorption structure. This structure uses the U, U-shaped recessed portion 15 2 and the &quot; U, the cooperation of the shaped electrode sheet 1505 enables the first printed conductive strip 1 5 08 which is connected to the working electrode to be printed on the insulating sheet at the same time as the second and third printed conductive strips 1511 and 1514. One side, thus eliminating the need for a printing process. The chemical reagent 1 5 1 7 is placed on the top surface of the working electrode 1 5 05 and is used to generate a chemical reaction with an analyte in the fluid to be measured and generate an electrical signal, and output the electrical signal through the electrode 1505 To the signal output terminal 1510. The material of the three printed conductive strips 15 0 8, 1 5 1 1, 1 14 may be a carbon-containing conductive paste or silver paste, and the reference number 15 2 3 refers to a fluid inlet. If it is viewed from another level, the printed conductive strip 10 in the tenth figure can be changed into a one-piece conductive strip or eliminated, please refer to the sixteenth figures (a)-(c) This is an application example of another three-electrode detection sheet with capillary flow channels according to the present invention. The electrode active end, electrode lead wire, and electrode output end of the three electrodes in this embodiment are all one-piece conductive sheets. An insulating sheet 1601 Has a first side 1602 and a second side

200422609

V. Description of the invention (30) 1603 'There is a first recessed portion 1604 and a through hole 1605 on the second surface 1603 (see the sixteenth figure (c) for details), the through hole 1605 and the first The surface 1602 communicates with each other; a first conductive strip 1606 integrally formed is placed in the first recess 1604 and forms a tight fit. The first conductive strip 1606 has a first electrode output terminal 608 and a first The electrode terminal 1607 uses the first electrode terminal 1607 to perform the function of a working electrode 1607. There is a second recessed portion 1611 and a third recessed portion 1610 on the first surface 1602 of the insulating sheet 1601. A second conductive strip integrally formed is placed in the second recessed portion 1611 and forms a tight fit. The second conductive strip 1616 There is a second signal output terminal 1618 and a second electrode terminal 1617 on the top. A pair of electrodes 1617 are formed by the second electrode terminal 1617, and a third conductive strip 1 6 1 2 formed in one is placed in the third recess 1 6 1 0 and form a tight fit. The third conductive strip 1612 has a third signal output terminal 1614 and a third electrode terminal 1613. 俾 Use the third electrode terminal 1613 to perform the function of a reference electrode 1613. The reference electrode 16 1 3 After being modified by a silver / silverized silver modified layer 1615, it has become a silvered silver reference electrode. An insulating layer 1619 has a &quot; C ', opening 1 620 and covers the two conductive strips 1614, 1618. Only the electrode active surface 1607 and the electrode output terminal 1680 are exposed. A cover layer 1 6 2 1 has a capillary convection air hole 1 6 2 2 and covers the Cπ-shaped opening 16 2 0 to form a complete capillary adsorption structure. A chemical reagent 1 60 9 is placed on the working electrode. The top surface of 1 6 07 is used to produce a chemical reaction with an analyte in the fluid to be measured and generate an electrical signal, and the electrical signal is output to the signal output terminal 1608 through the electrode 1607, and the reference number 1 6 2 3 is Refers to a fluid inlet.

Page 33 200422609 l

V. Description of the invention (31) Please refer to the seventeenth drawings (a)-(b), which is an application example of another three-electrode detection sheet with a capillary flow channel according to the present invention. Modification of the embodiment. In the sixteenth figure, the electric &amp; and beam test electrodes 1613 are printed on the insulating sheet ΐ600 by a conventional printed electrode method: wherein the reference electrode 1613 needs to undergo a silver / silver chloride modification process. Since the thallium electrode 1613 and the working electrode 1660 are also attached to the same insulating sheet 16> 0, and are afraid that the working electrode 1 6 07 will be contaminated during the silver / silver chloride modification process, this implementation For example, the counter electrode 1617 and the reference θ electrode; 613 were printed on the cover layer 1 71 6. During the modification process of the silver / silver chloride, the cover layer 1716 and the insulating sheet 1701 attached to the working electrode 1 707 were used. It is separated, so the working electrode 1 707 will not be contaminated by the modification process of making the silver / vaporized silver. An insulating sheet 1 701 has a fluid measuring recess 丨 2 on it, and the bottom mask of the fluid 1 measuring recess 1 7 0 2 has a chemical reagent placing recess 丨 3 for placing a chemical reagent 1 708, a through hole 1704 is provided on the bottom surface of the reagent placement recess 丨 703. The through hole is used to place a first electrode 1707. The first electrode 1707 forms a tight fit in the through hole 1704. The first electrode 1707 is electrically conductive. The material is covered with a metal thin film to form a metal electrode 1707. The first electrode 1707 has a first electrode active surface and a first electrode interface. The first electrode active surface is used to perform a working electrode. A first conductive strip 1718 is placed on the bottom surface of the insulating sheet 1701. The first conductive strip 1718 has a conductive strip connecting end 1719 and a signal output end 1720. The connecting end 1719 of the first conductive strip 1718 is electrically connected to the first electrode connecting mask ’, so that a working electrode measurement is performed by using the signal output terminal 1720.

Page 34 200422609

The signal 'fluid measuring recess 1 7 02 has a fluid inlet 17 05 and a capillary convection air hole 1 7 06. A covering layer 1 71 6 is placed above the fluid measuring recess 1 7 0 2 to form a Adsorption structure with complete capillary phenomenon. A second conductive strip 丨 7 丨 9 and a third conductive strip 丨 7 丨 3 are printed on the cover layer 丨 7 丨 6. The second printed conductive strip 1709 has a pair of electrode output terminals 1711 and a second electrode terminal mo as a The counter electrode 1710 and the third printed conductive strip 7 13 have a reference electrode wheel outlet 1715 and a third electrode end 1714 as a reference electrode 1714, and the reference electrode 1 71 4 is subjected to a silver / silver chloride modified layer 7 1 After the modification of 2, the synthesis was made as a — gasified silver reference electrode. A chemical reagent 1 70 8 is placed on the top surface of the working electrode 17 07 and is used to generate a chemical reaction with an analyte in the fluid to be measured and generate an electrical signal, and output the electrical property through the electrode 1 70 7 The signal goes to the signal output terminal 72, and the reference numeral 1 71 7 refers to a "C &quot; shaped opening. Please refer to Fig. 18 (a)-(c), which is the application of another three-electrode detection sheet of the present invention. For example, in this embodiment, the back of a metal electrode is directly used as a signal output terminal, and the metal signal output terminal is directly connected to an input connection terminal of a measuring instrument. An insulating sheet 1 801 (that is, an insulating substrate) has a The bottom of the fluid measuring recess 1 802 has three through holes 1803, 1804, and 1805, and the three electrodes 1808, 1809, and 1810 are placed in the through holes 1803, 1804, and 1805 and formed. Closely matched, each electrode 1808, 1809, 1810 has an electrode signal output surface 1813 and an electrode active surface 1 8 11 '. Using the electrode active surface 1 8 11 performs an electrode function, and a chemical reagent 1814 is placed on the electrode active surface 1811. Above. A mesh sheet 1 8 1 5 placed chemical reagent丨 8 丨 4 above to protect chemistry

200422609 V. Description of the invention (33) Reagent 1 8 1 4 and filtering out impurities in the fluid to be measured, a covering layer 丨 8 丨 6 covers the mesh sheet 1 8 1 5 and the insulating sheet 1 8 0 1 Overlay! 8 i 6 has an opening 1 8 1 7 as the inlet for the fluid to be measured. There are clips 1 80 6 and 1 807 (ie, tenon) on both sides of the insulating sheet 1 8 〇1, and the clips 1 8 0 6 and 1 80 7 are used to slide the sheet 1801 of the embodiment of the present invention into One of the eighteenth figure (d) of the measuring instrument 1 820 is inserted into the slot 1821 to form a positioning function. After the sheet 1801 is inserted into the card slot 1821, the signal output contact 1813 of the test piece 1801 is directly contacted with the input signal connection point 1822 of the measuring instrument 1820, and the signal transmission can be completed 'without using a conductive strip 1 006, of which The reference number 1 81 8 refers to the bottom surface of a sheet. If the metal electrode of the disposable sensor made according to the present invention is used as a working electrode, it can be applied to "Electron-Transfer Mediator Modified Working Electrode" And "Metal-Catalyzed Electrode". As long as the first type of metal electrode is an inert metal without chemical interference (Nobie Metal), it is not limited to any precious metal electrode (such as gold, platinum, palladium, rhodium, etc.), the present invention In the structure of such an electrode, a noble metal film 404 is used to cover a conductive material 403 to synthesize a metal electrode 411, and the metal electrode 4 11 is placed in a through hole 402 of an insulating sheet σ 401, which can reduce the noble metal. The use amount is reduced, the manufacturing man-hours of the metal electrode are reduced, and the sensor can obtain a stable and accurate electrode area to obtain good quality detection reproducibility (R epr Oducibi 1 ity). As for the second type of metal catalytic electrode, the working electrode material used must be

200422609

V. Description of the invention (34) In order to participate in electrochemical catalysis (without the need to add an electron transfer medium to the chemical reagent), the material must be compatible with the chemical reagent and the analyte to be tested. Different metal materials can be used. The metal electrodes are not limited to inert noble metal materials. For example, copper electrodes can be used as working electrodes for detecting H202. When the material of the metal electrode used is not expensive, the structure of the present invention can directly select the same material as that of the conductive material 403 and the metal electrode 411, and the procedure of covering the metal film 404 can be omitted. The structure and method can still be reduced &quot; reducing the manufacturing man-hours of the metal electrode, and enabling the sensor to obtain a stable and accurate electric and area, and obtain good quality detection reproducibility, which is similar to the prior art in the structure and manufacturing process of the electrode The structure of the method of disposable metal electrodes is greatly improved. ^ If the thirteenth figure is viewed from another technical type, the present invention is a method for manufacturing a disposable electrochemical sensing test sheet, which includes the following steps to provide an insulating sheet 1301 having at least two recesses (through holes) 1032, preparing a conductive material group, which has at least a first and a second conductive material clothing (ie, the original material of the electrode 1 3 1 3), at least the first conductive material is modified to form a modified electrode 1315 〇315 can be electroplated with a platinum film as a working electrode, electroplated with a silver / silver chloride layer as a reference electrode,), and placing the modified electrode 1315 and the second conductive material in the two recesses 1 302, Rhenium forms the disposable chemosensory test piece. The sensing test piece further includes a plating process, which forms a metal thin film on the first conductive material to the modified electrode 1315. The sensory test piece further includes a chemical reagent fixing procedure to obtain an enzyme electrode.

Page 37 200422609 V. Description of the invention (35)-The inventor of the present invention designed the structure and manufacturing method of the present invention based on the principle of not wasting precious metal materials. The present invention uses a low-cost conductive material 40 3 (such as cylindrical copper particles, the diameter of which is 15 mm, and the thickness is 0 5 mm) to facilitate a layer of inert noble metal film (such as platinum of the electric mine, and the thickness of the coating is 0 · 0 25-0 · 075 / zm) to form a noble metal electrode 4n, put the electrode 4 丨 1 into the through hole 402 of an insulating sheet 401 and form a tight fit, so that the electrode 411 is covered by the insulating sheet 401 and only an electrode active surface 409 is exposed. And an electrode interface 4 0 8 ′, using the electrode interface 4 0 9 to perform an electrode function, and then printing a conductive strip 410 to interface with the interface 408 of the electrode 411, as the guide line 410 and output of the electrode 411 End 4 0 6. The precious metal material used in this structure is only the electroplated thin film layer 404, and it is limited to the electrode area 412 in the through hole 402. This can reduce the use of shell metal 1 to a minimum, and it can electroforge millions of grains at a time. After the electrode 411, it is then machined into the through-hole 4 0 2 of the insulating sheet 4 ο 1, so the material and manufacturing cost of the precious metal electrode can be greatly reduced. In addition, the electrode 411 of the present invention is placed in the through hole 40 of the insulating sheet 401 to form a tight fit. The insulating sheet 401 tightly covers the side of the electrode 4 11 and only an electrode active surface 4 9 and an electrode are exposed. The connecting surface 4 008, and the conductive strip 41 is printed. The connecting surface 408 is connected to the electrode 411. The guiding line 410 and the output terminal 406 of the electrode 411 are not in direct contact with the electrode active surface 409, so that the area of the electrode active surface 409 It is completely independent and determined by the area of the through hole 402, and a very accurate electrode area 4 1 2 can be obtained. Since the measurement current of the electrochemical sensor is proportional to the electrode area 41 2, the present invention can make electrochemical sensing The reproducibility of the measuring signal (Reproducibi 1 it ty) has been greatly improved. In the present invention, the sensor insulation sheet 4 can be formed by plastic injection.

200422609 5. The electrode through hole 402 of the description of the invention (36) can be used to form other mechanisms required by the sensor at the same time using the same plastic injection procedure, such as the inlet of the fluid to be measured, the groove of the capillary flow channel of the fluid. 1314, capillary convection pores 13〇9, chemical reagent 1307 placement recess 1306, abutment bump 1316, and other structures at one time ^, not only can reduce the number of component assembly to reduce costs, but also reduce multiple components Assembly error. / In summary, the present invention can indeed form a metal thin film on a conductive material in a brand-new way to reduce the amount of metal electrode metal used and the installation of the metal electrode used in = The consistent hole width shortens the two rooms needed to make the abandonment test piece, and the industrial production. Therefore, anyone who is familiar with this technique a thinks and modifies it in various ways.

200422609 Brief description of the diagram Brief description of the diagram The first diagram: a schematic perspective view of an electrochemical detection device of the prior art; the second diagram: a schematic front perspective view of another prior art discarding metal electrode sensor; The third figure: is a schematic perspective view of another type of disposable metal electrode sensor manufactured by the metal precipitation method in the prior art; the fourth figure (a)-(d): the single electrode of the electrochemical sheet sensor of the present invention A three-dimensional front view, a three-dimensional back view, a three-dimensional front view, and a three-dimensional view of a conductive material covering a metal film of a preferred embodiment of the structure. Fifth figures (a)-(b): the electrochemical formula of the present invention Three-dimensional front view and three-dimensional explosion front view of another preferred embodiment of the structure of the single electrode of the thin-film sensor; Figures 6 (a)-(c): The single-electrode of the electrochemical thin-film sensor of the present invention Another preferred embodiment of the structure is a three-dimensional front schematic diagram, a three-dimensional back schematic diagram, and a three-dimensional burst back schematic diagram; FIG. 7 (a)-(b): is an electrochemical sheet sensor of the present invention. Another preferred embodiment of the electrode structure is a three-dimensional front view of the three-dimensional explosion and a three-dimensional back view of the three-dimensional explosion; FIGS. 8 (a)-(b): a three-dimensional front view of the structure of a modified electrode of a single electrode of the present invention and Schematic illustration of a three-dimensional frontal explosion; Ninth diagram: A schematic illustration of a three-dimensional frontal surface of an application example after combining the single electrode and the modified electrode of the present invention;

200422609 Brief description of the drawings Tenth drawings (a)-(b): It is a three-dimensional front schematic diagram and a three-dimensional frontal explosion schematic diagram of another preferred embodiment of the structure of the double electrode of the electrochemical thin-film sensor of the present invention; A picture (a)-(b): It is a three-dimensional front view and a three-dimensional front explosion view of another preferred embodiment of the three-electrode structure of the electrochemical sheet sensor of the present invention. The twelfth view: the present invention Three-dimensional frontal schematic diagram of an application example of a three-electrode detection sheet; Figures 13 (a)-(d): It is a preferred implementation of a sheet sensor with a capillary channel (suitable for detecting trace fluid samples) of the present invention 3D front view, 3D back view, 3D front burst view, and 3D schematic view of a metal thin film modified by a conductive material; ® 0 means C-face a): the tenth example of the positive C figure Practical Mingfa should be combined with the meter to measure-with thin film test chart, the five sense film thin type of chemical electricity of Mingfa is shown on the right side, the example of the body image is intended to explode the real surface The back of the body is shown separately and the structure of the open pole is shown in the open pole. The three positive individuals are set up separately, and the diagram is intended: the other is the C) structure of the C junction. The display of the second sensor film is based on the example of physical education, and Shi Shijia is more willing to show the face and the back of the body and: Tulisi b) Show C On-Explode a) Face C is positive The figure body is tenth and tenth, and the figure is intended to show that the hair is bright and clear. It is compared by Tong Tongshi Shijia; the machine map is intended to show the thin and explosive surface. C case with micro-display surface examination

Page 41

200422609 Brief description of the drawings Eighteenth drawings (a)-(d): It is a three-dimensional front schematic diagram, a three-dimensional front bursting schematic diagram, a three-dimensional back schematic diagram and the implementation of the present invention of another preferred embodiment of the sheet sensor of the present invention. Example is a three-dimensional front view of a combination of a measuring instrument.

Page 42

Claims (1)

200422609 VI. Application Patent Scope K—A kind of disposable chemical-sensing strip (Electrochemical Sen-sor Strip), which includes: an insulating sheet (Shee t), which has at least one through hole; at least one conductive material, which is installed in the In a through hole; a metal thin film covering the conductive material to synthesize an electrode, and the electrode having an electrode active surface and an electrode connection surface, and using the electrode active surface to perform an electrode function; and at least one printing A conductive strip is installed on the insulating sheet and has a conductive strip connection end and a signal output end, the conductive strip connection end is electrically connected to the electrode connection surface, and the signal output end is used to output the electrode function Generate a measurement signal. 2. The test strip as described in item 1 of the scope of patent application, wherein the conductive material is a metal, and a metal electrode is synthesized with the metal thin film. 3. The test strip as described in item 2 of the scope of patent application, wherein the material of the conductive material is a copper or an alloy thereof, such as brass, oxygen-free copper, bronze, phosphor copper, white copper, beryllium copper. 4 · The test strip as described in item 1 of the scope of patent application, further comprising a chemical reagent, which is arranged on the active surface of the electrode to detect a component of an analyte (A na 1 yte) in a fluid sample. The chemical reagent reacts with a component of the analyte, thereby generating a measurement signal, and outputting the measurement signal through the signal output terminal. 5. The test strip as described in item 4 of the scope of patent application, wherein the electrode forms an electrode area in the through hole, and the through hole area is a working surface area of the electrode (Working Surf ace Area) Active area Page 43 200422609 VI. Patent application scope and transfer the measurement signal. 6 · The test strip as described in item 1 of the scope of the patent application, wherein the printed conductive strip is printed on the insulating sheet with a conductive paste (Conductive Paste) to form the conductive strip connection end and the signal output end, The conductive strip is connected at the end and covered on the electrode interface to form an electrical connection with the electrode; wherein the conductive paste may be a carbon-containing, silver-containing, copper-containing, recording, chain-containing, gold-containing, non-recording steel. Any one or a mixture of conductive adhesives. 7 · The test strip as described in item 1 of the scope of patent application, further comprising an insulating layer for covering the printed conductive strip. 8 · The test strip as described in item 1 of the scope of patent application, wherein the material of the metal film is a gold, platinum, key :, silver, silver, copper, nickel, titanium, chromium, iron, or metal . 9 · The test strip as described in item 1 of the scope of the patent application, wherein the material of the conductive material is a carbon-containing plastic conductive composite material and a plastic material with a conductive coating treatment. 10 · The test strip as described in item 1 of the scope of the patent application, wherein the metal thin film is covered with conductive materials by means of electric recording, a dipping bell, a metal sink, printing or a metal spray paint. 11. The test piece described in item 10 of the scope of the patent application, the electroplating device contains a forging solution 'and the plating solution has a metal ion to form the metal film on the conductive material. 12. According to the test piece described in item 丨 丨 of the scope of patent application, the metal thin film is formed on the conductive material in advance by the electroplating device to synthesize the electrode, and then the electrode is placed on the insulating sheet. In the hole. Page 44 200422609 The scope of the patent application = the test piece described in item 11 of the scope, ★ The conductive material belongs to the through hole of the two pieces of ball belly and four edges of the edge, and then the gold iV is formed by the electroplating device such as: J conductive material to synthesize the electrode in the through hole. The test piece described in item 1 of the patent scope of M and π, wherein the thickness of the metal thin film may be 0.025 ~ 5. No. 2: The test piece described in item 1 of the scope of the patent application, wherein the through hole and the guide 1 fi are each a circle, a rectangle, a ring and form a tight fit. And the test piece described in item 1 of the right-shen patent range, wherein the insulating sheet has eight holes and the bottom is connected to form a "U &quot; shaped recess, which is used to form a tight surface of the conductive material to form a tight surface. Hey, the electrode surface is synthesized on the conductive material in one of the through holes in the state. The other side of the conductive material of the "U" -shaped carrier surface is the electrode and the surface is used to conduct electricity with the printing. The strip forms an electrical connection, 1 using this &quot;: ': two parts, structured so that the electrode active surface is formed with the printed seal strip: the same side of the thin edge of the edge, the edge, the through hole is a First, the printed conductive strip is a first metal thin film to synthesize the electrode, and then a working electrode is used. 1 · As described in the first patent application, the conductive material is a first conductive material and printed. Conductive strip, the metal film is a first electrode is a first electrode, and the function of the (Working Electrode) is used. Article 18: The test piece described in item 17 of the scope of patent application, further includes conductivity Material, which is set in the insulating sheet—hair 3 ^ ^ In the first one, a two metal thin film is placed on the second conductive material # ^, a second electrode is synthesized from the second electrode, the second electrode has a second electrode active surface and a _ Di Yi electrode balance interface, 面 200422609 VI. Scope of patent application '---- a &quot; ^-Spear J uses the first electrode active surface to perform the function of a pair of electrodes (Counter π tnode); and a second printed conductive strip, which The first printed conductive strip is provided with a second conductive strip connecting end, and the first output end is a first printed conductive strip. The second conductive strip connecting end is printed and covered on the second electrode connecting surface to form an electrical connection. . I! · The test strip as described in item 18 of the scope of patent application, further comprising a third conductive material and placed in a third through hole of the insulating sheet, a third metal, and a film placed on the third conductive A third electrode is synthesized on the material, the third electricity has a third electrode active surface and a third electrode interface, and the third electrode is used to perform the function of a reference electrode; and a third electrode The printed conductive strip is installed on the insulating sheet and has a second conductive strip connection end and a third signal output end. The third conductive strip balance end is printed and covered on the third electrode connection surface to form an electrical connection. . 2 0. According to the test piece described in item 9 of the patent application scope, the third metal thin chess material is a silver metal film, and the silver metal film is modified into a silver by immersion in a chemical solution or a printing device. Ag / AgCl Reference Electrode. 2 1 · The test strip as described in item 18 of the scope of patent application, has an inflow recess above the electrode of the insulating sheet for the inflow of the fluid sample, and a fluid inlet is provided on one side of the inflow recess. The fluid inlet, the inflow recess and the electrode placement through-hole are integrally formed. A cover layer is placed above the inflow recess of the insulating sheet to form a capillary flow channel for the fluid sample with the fluid inlet and inflow recess. And a measurement area, the inflow recess has another pair of capillary convection air holes to form the capillary flow with the fluid inlet 200422609 VI. Scope of patent application Convection stomata of the channel. 2 2 · The test strip as described in item 21 of the scope of patent application, which includes a pair of electrodes and a working electrode to form an electrode group, and has a recess in the bottom mask of the measurement area. It is the upper part of the electrode group to provide a place for the chemical reagent, and to form / form a uniform thickness coating. 2 3 · The test piece described in item 21 of the scope of patent application, has an abutment bump on the insulating sheet to abut the cover layer, and uses the abutment bump to isolate the fluid sample from the cover One layer of adhesive. 24. The test strip of the test strip described in item 21 of the scope of patent application, which includes a pair of electrodes' a reference electrode, a working electrode, a fluid test recess and a cover layer, and the pair of electrodes and the reference electrode are both A printed electrode formed by printing a conductive paste on the cover layer, and the working electrode is a metal electrode installed in the through hole on the insulating sheet. 2 5 · According to the test piece described in item 7 of the patent application scope, the first electrode further includes a modified layer, which is cured on the metal electrode to form a modified electrode. 2 6 · According to the test piece described in item 7 of the scope of patent application, there is a second printed conductive strip on the insulating sheet, the second printed conductive strip has a second electrode active surface and a second electrode signal output On the other hand, the second electrode uses the active surface of the second electrode to perform the function of a pair of electrodes (Counter Electrode). 2 7 · According to the test piece described in item 26 of the patent application scope, there is a third printed conductive strip on the insulating sheet, the third printed conductive strip has a third electrode active surface and a third electrode signal output Terminal, using the third electrode active surface to perform the function of a reference electrode (Reference Electrode), Page 47 200422609 VI. Application materials in &quot;-and use an electroplating or a printing device to modify the three-electrode active surface with a silver / silver chloride modified layer as a silver / gasification reference electrode (Ag / AgCl Reference Electrode) 〇 2 8-A type of disposable electrochemical sensing test strip, comprising: an insulating sheet, which has at least one through hole; at least one conductive material, which is installed in the through hole; and A metal film is covered on the conductive material to synthesize an electrode, and the electrode has an electrode active surface and a signal wheel exit end. The electrode active surface is used to perform an electrode function, and the signal wheel exit end is used to Outputting this electrode action generates a measurement signal. 29. The test strip as described in item 28 of the scope of patent application, further comprising a chemical reagent, which is arranged on the active surface of the electrode and is used to detect a component of an analyte in a fluid sample. A component of one of the analytes reacts to generate a measurement signal, and the measurement signal is output through the signal output terminal. 30. The test strip as described in item 29 of the scope of patent application, wherein the insulating sheet has a through hole to v to accommodate the electrode group, and the insulating sheet has two to place the test sheet in a measuring instrument On the other hand, the latch is fixed in one of the slots of the measuring instrument. 2 · The test strip described in item 30 of the patent scope on the right side, where each of the electrodes is an 8 尨 output contact to connect one of the measuring instruments to the signal connection. Above the electrode group of the insulating sheet is a measuring recess for a fluid sample.丨 The 1 measuring recess is integrally formed with the electrode placement through hole, and a mesh sheet is used to filter out impurities in the fluid sample in the measuring recess. The net is on the 48th page of the 200422609. The electrode group and the chemical reagent are provided to form a measurement area of the fluid sample, and a cover layer is placed above the mesh sheet and is in contact with the insulation sheet to protect the mesh sheet from falling. The cover layer has an opening for the drip point of the fluid sample. 3 2 · According to the test piece described in item 28 of the scope of patent application, the signal output end of the electrode has a riveted bump, and the test strip further includes a thin metal strip mounted on the insulating sheet, and The metal thin strip has a metal thin strip wheel exit end = an electrode connection hole, and the electrode connection hole is used to connect the riveted bumps for electrical connection. "啦 33 · —a kind of disposable electrochemical sensing test piece, comprising: an insulating sheet having at least one recess; at least one metal electrode installed in the recess, and the metal electrode having an electrode active surface and A signal output terminal, which uses the electrode as a surface to perform an electrode function, and the signal output terminal is used to output the electrode to generate a measurement signal. 34. The test piece described in item 33 of the scope of patent application It also includes a metal thin film, and the metal thin film is integrally formed with the metal electrode. 35. The test piece described in item 34 of the scope of patent application, further includes a conductive strip, and the conductive strip is connected to the metal electrode. It is integrally formed in the recess of the edge sheet. 3 6 · A disposable electrochemical sensing test sheet comprising: an insulating sheet having at least one through hole; at least one metal electrode installed in the through hole And the metal electrode has an electrode active surface and an electrode connection surface, and the electrode active surface is used 200422609 6. The scope of the patent application is to perform an electrode function; and at least one printed conductive strip is installed on the insulating sheet and has a conductive strip connection end and a signal output end. The conductive strip connection end is electrically connected to the The electrode interface, and the signal output end is used to output a measurement signal generated by the electrode action. 37. The test piece described in item 36 of the scope of patent application, wherein the metal electrode is a copper electrode. 38 · —a disposable electrochemical sensing test piece, comprising: an insulating sheet having at least one through hole; and at least one metal electrode installed in the through hole, and the metal electrode has an electrode active surface And a signal output terminal, the electrode action surface is used to perform an electrode action, and the signal output terminal is used to output a measurement signal generated by the electrode action. For example, the test piece described in the application for the β-month patent | &amp; item 38, wherein the insulating sheet HJ has two through holes to accommodate the electrode group, and the insulating sheet has: ::, α The piece is placed on a measuring instrument, and the tenon is quilted; it is set in the slot of the measuring instrument. A manufacturing method of a disposable electrochemical sensory test piece, which includes the following steps: an insulating sheet having at least two recesses, a material preparation, and a conductive material group having at least a first and a second conductive modification The first conductive material to form a modified electrode; 200422609 6. Scope of patent application The modified electrode and the second conductive material are placed in the two recesses to form the disposable chemical sensing test piece. 41. The method as described in item 40 of the scope of patent application, further comprising a plating process for modifying the first conductive material to form a metal thin film to obtain the modified electrode. 4 2. The method according to item 40 of the scope of patent application, further comprising using a printing program to print at least one conductive strip to form at least one signal output terminal, and electrically connecting the output signal of the electrode to the signal output end. 4 3. The method according to item 40 of the scope of patent application, further comprising a chemical reagent curing process for modifying the electrode to obtain an enzyme electrode. Page 51