SU903823A1

SU903823A1 - System for micro-organism cultivation automatic control

Info

Publication number: SU903823A1
Application number: SU802919545A
Authority: SU
Inventors: Римвидас Юозович Симутис; Юргис-Казимерас Юргевич Станишкис; Ляонас-Ромуальдас Антанович Коцюс
Original assignee: Каунасский Политехнический Институт Им.Антанаса Снечкуса
Priority date: 1980-05-05
Filing date: 1980-05-05
Publication date: 1982-02-07

Description

Изобретение относится к медицинской технике, а именно к микробиологии, в частности к устройствам управления процессом ферментации.The invention relates to medical equipment, namely to microbiology, in particular to devices for controlling the fermentation process.

Известна система автоматического управления процессом культивирования микроорганизмов, включающая датчик pH, связанный через регулятор pH с исполнительным механизмом, установленным на линии подачи аммиачной воды и исполнительный механизм, установленный на линии подачи питательных солей[ί].A known system for automatic control of the process of cultivation of microorganisms, including a pH sensor connected through a pH regulator with an actuator mounted on the ammonia water supply line and an actuator mounted on the nutrient salt supply line [ί].

Однако известная система не обеспечивает необходимой точности регулирования подачи питательных солей изза отсутствия контроля скорости роста и концентрации микроорганизмов.However, the known system does not provide the necessary accuracy of regulating the supply of nutrient salts due to the lack of control of the growth rate and concentration of microorganisms.

Цель изобретения - повышение точности регулирования.The purpose of the invention is to improve the accuracy of regulation.

Указанная цель достигается тем, что система автоматического управления процесс.ом культивирования микроорганизмов, включающая датчик pH, связанный через регулятор pH с испол нительным механизмом, установленным на линии подачи аммиачной воды и исполнительный механизм, установленный на линии подачи питательных солей, снабжена последовательно установленными блоками определения параметров автоколебаний pH и определения скорое ти роста микроорганизмов, при этом вход блока определения параметров автоколебаний pH подключен к регулятору pH, а выход блока определения скорости роста микроорганизмов через дополнительный регулятор связан с ист полнительным механизмом, установленным на линии подачи питательных солей. .This goal is achieved by the fact that the system of automatic control of the process of cultivating microorganisms, including a pH sensor connected via a pH regulator with an actuator installed on the ammonia water supply line and an actuator installed on the nutrient salt supply line, is equipped with sequentially installed detection units pH auto-oscillation parameters and determining the growth rate of microorganisms, while the input of the pH auto-oscillation parameter determination unit is connected to the pH controller, and Exit determination unit microbial growth rate by an additional controller connected with ist additionally mechanism installed on the supply line of nutrient salts. .

На чертеже изображена структурная схема системы автоматического управления процессом культивирования микроорганизмов.The drawing shows a structural diagram of a system for automatic control of the cultivation of microorganisms.

Система автоматического управления процессом культивирования микроорганизмов содержит датчик pH 1, свя¹· занный через регулятор pH 2 с испол903823 нительным механизмом 3, установленным ria линии подачи.аммиачной воды, исполнительный механизм 4, установленный на линии подачи питательных солей, блок 5 определения параметров автоколебаний pH, блок 6 определения скорости роста микроорганизмов/ при этом вход блока 5 определения параметров автоколебаний pH подключен к регулятору pH 2, а выход блока 6 опре- ¹⁰деления скорости роста микроорганизмов, через дополнительный регулятор 7, связан с исполнительным механизмом 4, установленным на линии подачи питательных солей.System of automatic control of the process comprises culturing microorganisms pH sensor 1, ¹ · svya coupled through a regulator to pH 2 ispol903823 tional mechanism 3 installed ria podachi.ammiachnoy water line, the actuator 4 mounted on the supply line of nutrient salts determining unit 5 oscillations parameters pH block 6 determine the growth rate of microorganisms / wherein the input determination unit 5 oscillations parameters pH controller connected to the pH 2, and the output of block 6 determine ¹⁰ dividing the growth rate of microorganisms through d an additional regulator 7 is connected to the actuator 4 mounted on the feed line of nutrient salts.

Система работает зсм.The system runs zsm.

следующим обраСигнал с датчика pH 1 среды поступает на позиционный регулятор pH 2, который сравнивает заданное значение pH среды с текущим и формирует сигнал для исполнительного механизма 3, включающего или выключающего подачу аммиачной воды в ферментер. В зависимости от зоны нечувствительности регулятора в системе появляются автоколебания pH среды с постоянной амплитудой А = дрН. При этом скорость снижения pH среды пропорциональт на скорости роста микроорганизмов, а ³⁰бкорость увеличения pH среды пропор4The signal from the pH sensor 1 of the medium is fed to the position controller pH 2, which compares the set value of the pH of the medium with the current one and generates a signal for the actuator 3, which turns on or off the supply of ammonia water to the fermenter. Depending on the deadband of the regulator, self-oscillations of the pH of the medium with a constant amplitude A = drN appear in the system. The rate of decrease in pH of the medium is proportional to the growth rate of microorganisms, and ³⁰

Если поддерживать постоянным, то скорость роста микроорганизмов определяют уравнением й^рН('₂-Ч>/Ц ’ (₂)If kept constant, then the growth rate of microorganisms is determined by the equation th ^pH (' ₂ -H> / C' ( ₂ )

В блоке 5 определения параметров· автоколебаний pH среды измеряется продолжительность увеличения и продолжительность, снижения pH среды длл текущих автоколебаний. В блоке 6 определяют скорость роста микроорганизмов по уравнению (2). Сигнал с блока 6 подается на регулятор 7, задание которого меняется согласно с величиной подаваемого сигнала. Регулятор 7 формирует сигнал в зависимости от которого исполнительный механизм увеличивает или уменьшает подачу питательных солей в ферментер.In block 5 for determining the parameters of · self-oscillations of the pH of the medium, the duration of the increase and the duration, decrease in pH of the medium for the current self-oscillations are measured. In block 6 determine the growth rate of microorganisms according to equation (2). The signal from block 6 is fed to controller 7, the task of which changes according to the magnitude of the supplied signal. The controller 7 generates a signal depending on which the actuator increases or decreases the supply of nutrient salts to the fermenter.

Таким образом, предложеннаясистема автоматического управления повышает точность регулирования процесса культивирования микроорганизмов, что позволяет лучше использовать пи²⁵ тательные соли и увеличить выход готовой продукции.Thus, automatic control predlozhennayasistema improves the accuracy of control microorganism cultivation process, which allows a better use of pi ²⁵ tatelnye salt and increase the output of finished products.

Claims

The invention relates to medical technology, namely to microbiology, in particular to devices for controlling the process of fermentation. A known system for automatically controlling the cultivation process of microorganisms, including a pH sensor connected through a pH regulator with an actuator installed on the ammonia water supply line and an actuator installed on the nutrient salt supply line l However, the known system does not provide the necessary accuracy of regulating the supply of nutrient salts because of lack of control of growth rate and concentration of microorganisms. The purpose of the invention is to improve the accuracy of regulation. This goal is achieved by the fact that the system for automatic control of the process of cultivation of microorganisms, including a pH sensor connected via a pH regulator with an actuator installed on the ammonia water supply line and an actuator mounted on the supply line of nutrient salts, is equipped with sequentially installed determination units parameters of self-oscillations of pH and determining the rate of growth of microorganisms, while the input of the unit for determining the parameters of self-oscillations of pH is connected to the regulator pH, and the output unit detecting microorganism growth rate through the additional controller associated with ISH fillers mechanism installed on the supply line of nutrient salts. The drawing shows a structural diagram of an automatic control system for the cultivation of microorganisms. The system for automatic control of the cultivation of microorganisms contains a pH 1 sensor connected through a pH regulator 2 to an executive mechanism 3 installed on the supply line of ammonia water, an executive mechanism C installed on the supply line of nutrient salts pH , block 6 for determining the growth rate of microorganisms, f, while the input of block 5 for determining pH self-oscillation parameters is connected to a pH 2 regulator, and output for block 6 for determining the growth rate of microorganisms of additional controller 7, associated with the actuator 4 mounted on the supply line of nutrient salts. The system works in the following way. The signal from the pH sensor 1 of the medium is delivered to the positional pH regulator 2, which compares the set value of the pH of the medium with the current one and generates a signal for the actuator 3, which turns on or off the supply of ammonia water to the fermenter. Depending on the dead zone of the regulator in the system, self-oscillations of the pH of the medium with a constant amplitude A drH appear. At the same time, the rate of decrease in the pH of the medium was proportional to the growth rate of microorganisms, and the tKOpocTb increase in pH of the medium is proportional to the difference between the rates of supply of ammonia and the growth of microorganisms. Therefore, the values of pH and pHj, the medium are reached for a certain time interval t, f and, accordingly, with the equations MX, pH / t | ApH / t2, the time for increasing the acidity of the medium from pH to. the time of acidification of the medium from the value of pI to pH; ammonia water feed rate for alkalization of the medium; k, k2. constant coefficients; the amplitude of pH fluctuations determined by the insensitivity zone of the on-off regulator 3 If QjsjH is kept constant, the growth rate of microorganisms is determined by equation (2) X CjJ / in block 5 for determining the parameters of self-oscillations of the medium pH and the duration of Reducing the ERP environment for current self-oscillations. In block 6, the growth rate of microorganisms is determined by equation (2). The signal from block 6 is supplied to the regulator 7, the setting of which varies according to the magnitude of the input signal. The regulator 7 generates a signal, depending on which the actuator increases or decreases the supply of feed salts to the fermenter. Thus, the proposed automatic control system improves the accuracy of regulating the cultivation process of microorganisms, which makes it possible to better use the nutrient salt and increase the yield of finished products. The invention The system for automatic control of the cultivation process of microorganisms, which includes a pH sensor connected via a pH regulator to an actuator installed on the ammonia water supply line and an actuator mounted on the nutrient salt supply line. characterized in that, in order to increase the regulation accuracy, it is equipped with consistently installed pH self-oscillation parameters determination units and microorganisms growth rate determination, the input of the pH self-oscillations parameter determination unit connected to the pH regulator, and the output of the microorganisms growth rate output unit through an additional the controller is connected to an actuator mounted on the supply line for nutrient salts. Sources of information taken into account in the examination 1. USSR Author's Certificate No. 527k72, cl. P. 12 В 1/08, 09/05/76.

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Nutritional Nutrition