SU884574A3 - Method of preparing protein from microorganism suspension - Google Patents

Abstract

METHOD OF RECOVERING PROTEIN OF LOW NUCLEIC ACID CONTENT FROM MICROORGANISMS Proteins of reduced nucleic acid content are obtained from micro-organisms by thermal treatment comprising heating an aqueous suspension of the micro-organism with the optional addition of a material having ribonuclease activity, at a pH value between 7 and 8.5 and at 63 to 67.degree.C, until the 5'-nucleotide content in the medium has reached the desired level, then briefly boiling the resulting mixture and separating the insoluble crude protein from the suspension.

Description

New and varies mainly from 20 minutes to 20 hours.

The method involves the use of all types of microorganisms, i.e. yeast, bacteria, fungi, etc. So, Saccharomyces cerevlsiae, Candida boldinii, Nocardia erytropolis, Aspergillus niger microbacteria for mold fungi are used as yeasts.

Microorganisms are used as treated, for example, dried under mild conditions or lyophilized single-cell or untreated. According to this method, the heating time is reduced by administering biological preparations containing ribonuclease, for example, malt sprouts of herbs and cereals, in particular malt sprouts or extracts from

of them.

one

The decrease in the RNA content in the unicellular protein reaches 0.2. weight. % protein loss is insignificant and its output practically reaches 90% and more. A particular advantage of the method is that ribonucleic acid is almost completely decomposed into 5-nucleotides, which themselves are quite valuable substances. In addition, the filtrate used to obtain nucleotides after protein separation can be reused for growing microorganisms to produce nucleic acids, i.e. in this way, a practically circulating nucleotide cycle is obtained. At the same time, microorganisms for cell growth utilize the cell components present in the solution, residual soluble protein, etc., which leads to a decrease in the biotin and lactic acid additives used in the cultivation of microorganisms.

The resulting protein according to the proposed method is cleaner, better digested, contains detergents of salts or RNA, has no odor and can be used as a food product.

Example. 200 l of an aqueous suspension of the microorganisms listed in Table 1, with a dry mass content of 15–20%, are heated and melted at this temperature for 15 hours while stirring. The pH is maintained between 7-8.5 by addition of NaOH. The mixture is then briefly boiled and the crude protein is separated and dried.

The yield is 25-32 kg of raw.

e protein. Table 1 shows the RNA content of the starting material and the RNA cleavage achieved therewith. PRI mme R 2. a) 3 kg of malt sprouts are ground, 30 l of HlO are added and the mixture is kept for 40 min. The resulting suspension, either directly or after the separation of insoluble elements of malt sprouts, is applied in a centrifuge.

5 in the following process.

b) 200 l of a suspension of the same microorganisms as in example 1 with the same solids content is heated to, a suitable suspension of malt sprouts or extract is added (example 2a) and then the mixture is heated for 2-15 h at 65 s. The treatment is then carried out as in Example 1. The output is 25-35 kg of sulfur. royal protein, the average pure protein content of about 70%.

Table 2 shows the achieved RNA cleavage. The output of 5-nucleotides with an approximate 10% RNA content in the dry product 7-800 g each of

0 of four different 5-nucleosides, with a lower RNA content in the starting material, they were respectively lower.

Biomass for example 2

Saccharomyces cerevisiae

 carlsbergensis

Candida boidihii (approximately age

Nocardia erytropolis

Bacillus cereus (age 1 year)

Streptococcus faccalis

Pseudomonas fluoreszens Formula of the Invention A method of obtaining protein from a suspension of microorganisms by heating it at certain pH values with subsequent injection of the target product and 5-nucleotides, characterized in that in order to reduce the content of nucleic acids in the protein with simultaneous increase in the yield of 5-nucleotides, heating

table 2

Decomposition of RNA extract of malt sprouts

100% 90%

26%

100%

thirty%

100%

70% of the suspension is carried out for 215 hours at a temperature of 63-67 ° C and a pH of 7-8.5, after which a suspension of malt sprouts or their extract is introduced into it and the mixture is boiled for 0.5-5 minutes. Sources of information taken into account in examination 1, US Patent 3,720,585, cl. 260-112, published 1973. 2. The UK patent 1372870, cl. C 6 F, publish 1974 (prototype).

Claims (1)

Claims jo A method of obtaining protein from sous. microorganism suspension by heating it at certain pH values with subsequent isolation of the target 25 product and 5 * nucleotides, characterized in that, in order to reduce the content of nucleic acids in the protein while increasing the yield of 5 ¹ nucleotides, the supernatant is heated for 215 hours at a temperature of 63-67 ° C and a pH of 7-8.5, after which a suspension of malt sprouts or their extract is introduced into it and the mixture is boiled for 0.5-5 minutes.