SU785359A1 - Pseudomonas aeruginosa 1b strain - Google Patents

Description

The invention relates to the microbiological industry and relates to a new strain used in biochemical wastewater treatment of aniline-paint industries containing a mixture of synthetic organic compounds: aniline, cimethylaniline, naphthalene, o-nitrophenol, o-chitroanisole, d-nitrophenol.

Known strain PseudomontX S $ f> 1C for the treatment of industrial wastewater from alkyl sulfates £ 1J.

This strain destroys up to 7 g / l of dodecyl sulfate and up to 1 g / l of technical preparations of alkyl sulfates in the mineral medium.

Also known is the strain Pseudomonas putida K, used for the treatment of industrial wastewater from lithaupon and capable of destroying it up to 1 g / l in the mineral medium [2 ^.

However, known strains do not destroy a mixture of several compounds that are simultaneously in stock.

The aim of the invention is to expand the destructive capabilities of the strain of the destructor of synthetic organic compounds, for example, aniline, dimethylaniline, nitrobenzene, naphthalene, o-nit-rhenol, o-nitroanisole, p-nitroanisole., P-nitrophenol.

Strain Ps.aerugi notS0 (1B has the following morphological and physiological characteristics: direct motile rods, cells are located singly or in short chains, spores and capsules do not form.

On meat-peptone agar, the colonies are flat, round, translucent in transmitted light, with a diameter of 3-7 mm. Depending on the growth conditions and the composition of the medium, a greenish-blue pigment penetrates the substrate depending on the growth conditions. On meat-peptone broth, the culture causes a slight turbidity of the medium, forms a film and sediment. Emits pigment. Kovach oxidase test is positive. Glucose, mannitol, arabinose, xylose and '3 glycerin are fermented to form acids. Lactose, maltose, sucrose. sorbitol / salicin, dulcin, inositol does not ferment. It dilutes gelatin. Starch decomposes, restores nitrates, does not form indole and hydrogen sulfide. Absorbs mineral forms of nitrogen, does not decompose glucose under anaerobic conditions. Does not possess lecithinase. It does not have catalase activity.

The proposed strain is able to grow on a medium containing as a source of carbon and nitrogen a mixture of organic compounds - aniline, dimethylaniline, nitrobenzene, naphthalene, o-nitrophenol, P-phenyticin, o-nitroanisole, η-nitroanisole, I-nitrophenol.

The strain Ps.aeru ^ nosa 1B isolated from the drive of the cleaning shop Rubezhan production association Dye.

The strain is obtained as follows. To obtain an active destructor intended for wastewater treatment of aniline-paint industries in co *. the left medium of the following composition, g / l: MAnROd l; MH ₄ biO ₃ 1; KS t 0.5;

0.1; distilled water 1l introduced a sample from the drive of the purification station containing a mixture of organic compounds: aniline, dimethylMin, naphthalene, o-nitrophenol, p-phenytidine, o-nitroanisole, p-nitroanisole, p-nitrophenol.

The flasks with the medium were shaken on a rocking chair at 22 ° C for 7 days. COD - starting fluid decreased; by 63%. The resulting culture of accumulation was dispersed on meat-peptone agar and pure bacterial cultures were isolated. A culture capable of destroying the above components was sieved on oblique agar and the claimed strain was obtained.

Example. The use of the strain in the purification of water containing a mixture of synthetic organic compounds.

In an Erlenmeyer flask with a volume of 0.5 l was placed 200 ml of factory runoff containing the following components, mg / l: aniline 83.3; dimethylaniline 0.84; nitrobenzene 2.9; naphthalene 10.1; o- "itrophenol 0.23; p-phenicidine 3.3; o-nitroanisole 16.3; p-nitroanisole 0.1; p-nitrophenol 0.89; X ₍ -0.98; Chae-1.7. Cells ^{f of} strain Pz.aegi ^ xPuba 1B were added there in the amount of 10 ^ cells per 1 ml of culture medium. The flasks were incubated at 22 ° C on a shaker, pH 7.4.

After 7 days of incubation, there was a decrease in the concentration of organic compounds determined by gas-liquid chromatography. The change in initial concentrations of a mixture of organic compounds occurs as follows, mg / l: aniline 19.7; phenol 0.1; nitrobenzene 0.5; dimethylaniline 0.4; naphthalene 5.6; p-phenytidine 1.0; o-nitroanisole 3.6; p-nitroanisole 0.15; p-nitrophenol O; X ^ 0.24; X ^ 0.3.

COD decreased by 58%, the concentration of organic substances decreased by 28-100%.

As can be seen from the above example, the strain Pseudomonas aeruginasd-B is able to destroy a mixture of synthetic organic compounds. According to the authors, the treatment of the total runoff of the Rubezhansky Production Association Dye with activated sludge takes 9 days, while COD is reduced by 30%. The proposed strain reduces the COD of the factory runoff for 7 days by 58%, i.e., by reducing the concentration of synthetic organic compounds, the strain allows to intensify the purification by 2 times.

Claims (2)

7 glycerin ferments to form acid. Lactose, maltose, sucrose,. srbit, salicin, dulcin, inositol does not ferment. Gelatinu liquefies. Starch decomposes, restores nitrites, does not form indole and hydrogen sulfide. Absorbs mineral forms of nitrogen, in anaerobic conditions, glucose does not decompose. Leci does not possess ginase. It does not possess catalase activity. The proposed strain is able to grow on a medium containing as a source of carbon and nitrogen a mixture of organic compounds - aniline, aimethylaniline, nitrobenzene, naphthalene, o-nitrophenol, P-phenythidine, o-1 -troanisole, p-nitroanisole, p -nitrofenitol. The Ps.aeru inOSa 1B strain was isolated from the storage pool of the cleaning facility of the Rubezhansky Production Association Dye. Strain receive with; 1uchu way. In order to obtain an active destructor intended for sewage treatment of aniline dye industries in co. the left environment of the next co: tava ,, g / l: MANROD 11MNDMO 1; KCt 0.5; M5S. € n 0.1; distilled water of 1 l was added to the sample from the storage tank, containing a mixture of organic compounds: aniline, dimethylMine, naphthalene, o-itrophenol, p-phenytidine, o-nitroanisole, p-nitroanisole, p-nitrophenitol. Flasks with medium inlet 5 shvapi on a rocking chair at 22 C for 7 days. COD - source fluid decreased by 63%. The resulting accumulation culture was scattered on mono-peptone agar and pure bacterial cultures were isolated. A culture of rupture capable of destroying the above components was screened onto oblique agar and the claimed strain was obtained. Example. The use of strain in the purification of water containing a mixture of synthetic organic compounds. In an Erlenmeyer flask with a volume of 0.5 l of primvts1ali 200 ml of plant-wide stock containing the following components, mg / aniline 83.3; dimethylaniline 0.84; nitrobenzene 2.9; naphthalene 1O, 1; o-nitrophenol 0,23; p-fenitsilin 3,3; o-nitroanisole. 16.3; p-nitrbysynop 0,1; p-nitrophenitol 0.89; X) -0.98; Hae-1.7. Cells of the strain Rz.aoyi pa501 1B were added in the number of cells per 1 ml of the nutrient medium. Flasks were incubated at 22 ° C on a shaker pH 7.4. 94 After 7 days of incubation, there was a decrease in the concentration of organic compounds, as determined by gas-liquid chromatography. The change in the initial concentrations of the mixture of organic compounds is as follows, mg / l: aniline 19.7; phenol 0.1; nitrobenzene 0.5; dimethylaniline 0.4; naphthalene 5.6; p-phenitidine 1.0; o-nitroanisole 3.6; p-nitroanisole 0.15; p-nitrophenol O; X 0.24; X 0.3. COD decreased by 58%, the concentration of organic substances decreased by 28-1OO%. As can be seen from the above example, the strain PseueiotT onaS aepU iOasflilB can destroy a mixture of synthetic organic compounds. Purification of the total runoff of the Rubezhansky Production Association The dye, according to the authors, with the help of activated sludge occurs within 9 days, while the COD is reduced by 30%. The proposed strain produces a reduction in the COD of the plant-wide sewage within 7 days by 58%, i.e., by reducing the concentration of synthetic organic compounds, the strain makes it possible to intensify purification by 2 times. Claims of the invention: Pseudomonas aerug nasal B strain used for the treatment of wastewaters of aniline dye industries. Stored in the collection of the Academy of Sciences of the Ukrainian Academy of Sciences of Ukraine under No. 41. Morphological features: straight rods, cells arranged singly. long in short chains, gram-negative, spore and capsules do not form. Cultural characteristics: MPA colonies are flat, rounded, translucent in transmitted light, diameter 3-7 mm. Culture Depending on growth conditions and medium composition, forms a greenish-blue pigment that penetrates into the substrate. On the BCH culture causes clouding of the environment, forms a film and sediment. Pigment is excreted. Physical and biological characteristics: the culture is oxidase-positive. Glucose, mannitol, arabinose, xylose, and glycerin ferment to form acid. Lactose, maltose, sucrose, sorbitol, salicin, dulcite, innozit does not ferment. Gelatinu liquefies. Starch decomposes. Nitrates restores. Aerobe. 5785359 i Cultivar-1 optimal temperature, USSR author's testimony Neither 22 C. Strain is able to destroyNj 657063, cl. C 12 K 1/02, 1977. 28-100% mixture of organic synthetic compounds. 2. Copyright svktsvtelsgve USSR  Information, 5 per application Nj 2742215, taken into account during examination. C 12 K 1/02, 03/27/79,