SU739104A1 - Culture medium for p+virulence determinants - Google Patents

Description

This invention relates to the field of medicine, namely, "crobiology."

The known nutrient medium for detection of virulence determinants, containing agar, potassium phosphate monobasic, ammonium chloride, ammonium sulphate, galactose, B-phenyl-X-alanine, methionine, cysteine, valium, glycosine, sulfuric acid, magnesium ion, methionine, cysteine, valium, glycosine, sulfuric acid, methaline, methionine, cysteine, valium, sulfuric acid, galactose, B-phenyl-X-alanine, methionine, cysteine, valium, sulfate, ammonium chloride, galactose, ammonium chloride, ammonium chloride, ammonium chloride manganese, 4% solution of lactic acid, arginine, dye, growth factor and water 1.

However, the known medium is complex and costly.

The aim of the invention is to simplify the environment while maintaining its growth properties.

This goal is achieved by the fact that the medium contains ferrous sulphate ferrous acid as a growth factor, and Evans blue as a dye with a combined quantitative ratio of components (g / l of water):

Agar18.0-20.0

Potassium phosphate monoza-;

Metinous 4.0-4.2

Ammo chloride, 4-0.7%

Sulphate

0.4-0.7 ammonium 1.8-2.5 Galactose B-phenyl-A-ala0, 10-0.18 nin

Methionine 0.05-0.10 0.04-0.08 Cysteine

10 0.04-0.08 Valin 0.05-0., 10 Isoleucine 0.04-0.10 Glycine Sulfate

0.05-0.30 mg

15 Sulphate 0.0001-0,0005 zinc

Sulphate

0.0001-0,0005 manganese

Dairy

20

| o, 5-2.0 acid 0.01-0.16 Evans blue Ferrous sulphate gel, 3-1.0 oz Arginine

25 0.1-0.2.

The method is illustrated by the following example.

Example. To prepare 1 L of differential media with an advance

Claims (1)

30 pigeons 20 g agar agar soak in distilled water. The swollen agar-agar is washed, the water is changed for 2-3 days with at least 8-10 ra. The washed agar is melted by heating in 500 ml of water, the pH is adjusted to 7.0 with NaOH solution. Continuing to roar, add 4.12 g of KHjPO, dissolved in 300 ml of water at about 8.0 (by adding IN NaOH), 0.53 g dissolved in 100 ml of water and 0.53 g (NH jgSOq, also dissolved in 100 ml of water. The agar powder is brought to a boil, the pH is set to 7.4-7.5 and autoclaved at 120–20 minutes. After autoclaving, the agar is filtered and sterile for 1 liter of agar at a temperature the following medium levels are added: 2 g of galactose (10.0 ml of 20% solution) 120 mg / -fei-alanine (30 ml of 0.4% solution) 60 mg of methionine (5 ml of 1.2% solution) 48 mg of cysteine (5 ml of 0.96% solution ) 48 mg of the roller (5 ml of 0.96% solution ) 55 mg of isoleucine (15 ml of a 0.363% solution) 50 mg of glycine (5 ml of a 1% solution of 100 mg of MgSO (2.5 ml of a 4% solution of 0.1 mg of ZnSOij (1 ml of a 0.01% solution of 0.1 mg of MnSOq ( 1 ml of 0.01% solution) 40 mg of lactic acid (1 ml of 4% solution) 104 mg of arginine (10 ml of 1.04% solution) 30 mg of Evans blue (5 ml of 1% solution) 800 mg of FeSOii (16 ml 5 % solution The prepared medium is poured into Petri dishes, which are placed in a thermostat at 37 for 24 hours to check for sterility. Cups are sown at 200-500 m. the plague microbe strain studied and grown at 28 for 4-6 days. P colonies-those nosinie, P - bright. This medium can be modified by using growth as a growth stimulator at ri at a final concentration of 0.1% or Mora at a final concentration of 50-; 80 mg%, and as dyes - trypanog blue, Congo red or amide black at the end concentration 3-5 mg%. The use of the invention allows the P determinant of virulence of a smart microbe to be established using its accessible and cheap medium. Nutrient medium for detecting the virulence determinants of coagulant agar, potassium phosphate acid, sulfurous ammonium, ammonium sulphate, hashaktose, / nyl - / - alanine, methionine, cysteine, in, isoleucine, glycine, sulfuric acid, and alanine, alkydine, methionine, cysteine, in, isoleucine, glycine, sulfurousma, and alanine. % solution of molochislota, arginine, dye, product factor and water, which means that, in order to feel the medium while retaining its commercial properties, it contains, as a growth factor, sulfuric acid iron, as a filter, Evans blue with a slash of quantitative Nominal ratio of ponents (g / l of water): 18.0-20.0 Potassium phosphorus4, 0-4.2 monobasic acid mono-ammonium chloride 0.4-0.7 Ammonium sulphate 0.4-0.7 Galactose 1.8- 2,5-phenyl- / O, 10-0, 18 -alanine Methionine 0.05-0, 10 Cysteine 0.04-0.08 Valine 0.04-0.08 Isoleucine 0.05-0.10 Glycine 0 , 04-0.1 Sulphate 0.05-0.30 magnesium Sulphate 0,0001-0,0005 zinc Sulphate 0,0001-0,0005 manganese Milk sour0, 5-2,0 lots Evans blue 0.01-0, 10 Ferrous sulphate same0, 3-1.0 Lezo Arginine 0.1-0.2. Sources of information, ntye taken into account in the examination 1. J. Ehreg. Patho. (Brit.) 1956, VII, 6, 570-576.