SU623525A3 - Method of obtaining fructose - Google Patents

Description

The invention relates to enzymatic processes for the production of fructose. A method of producing fructose is known, which involves preparing an enzyme, saccharifying starch with a glucose amylase enzyme preparation to glucose, and then isomerizing the latter with a glucose isozyme to fraud, separating the solution from the enzyme purification and syrup concentration | ll, according to a known method for satisfactory glucose conversion, and the concentration of syrup | The dose requires at least 8 hours, provided that a sufficient copy is added to the enzyme. For the production of a fructose-glucose mixture from starch, the time npt of the process ranges from 128 to 14 O owls The purpose of the invention is to accelerate the process for the production of fructose. To this end, in the proposed method, saccharification of liquefied KpaxMaha to glucose and isomerization of glucose are carried out simultaneously by administering an enzyme preparation having glucozo-amylase and glucose-isomeric activity. For 1 kg of carbohydrates in the raw material, an enzyme should be added to a preparation that has 1050-units of glucose-amylase activity and 2OOO200OO units of glucose-isomerase activity. The process is expedient to ration at a temperature C and a pH of 5, O-7.5, preferably at 6 ° C-7 ° C and a pH of 6.0-6.5. The proposed method is as follows. Starch, liquefied by heating, converted by acid or an enzyme of microbial origin, for example bacterial or fungal amylase or coopode enzymes, is used as a starting material. Such liquefied starches can have a DE (dextrose equivalent) from 0 to 67. They can contain (a piece of up to 45% glucose. Paste of dried starch containing from 15 to 5% solids, preferably 30-40%, is heated to a temperature 4O- 80 s, preferably up to 6 ° C, and the pH is set at 5, O-7.5, mostly 6 e ,, 5, Hypюкzo-amylase and glucoeoiso me |) for, are added simultaneously in the compositions corresponding to 5O-500 EGA, preferably 75 -150 EGA, and 2OOO - l200OO EGI, preferably L 350012OOO EFHt per 1 kg of carbohydrates in pasta. One unit of EGI represents the amount of glucamylase, which) catalysin-n-5 per hour of dextrose per pH of pH 4.2 and 6O from soluble starch. One unit of EGA represents the amount of glucose isomerase, catalyzed the formation of fructose q of glucose cakes for 1 hour at a pH of 7.2, for the action of enzymes on starch molecules to gelatinize starch particles is inevitable, gelatinization occurs under the influence of heat and heat, and in some industrial devices shear forces are applied simultaneously, reduced by viscosity oh starch paste previously, may be added Ot enzymes with amylase activity prior to gelation, during it or immediately after it. Many of the about-amylases exhibit activity within the process conditions indicated above, and the joint action of viral glucose and glucose isomer. Therefore, the viscosity reducing action of d-amypase on gelatinized starch can be combined with the process of the invention. For this action, C-amylase activity is required in 4OOOO-80OOOO MBE (Vopigut modified units) per 1 kg of starch. Commercially available enzymes, purchased from manufacturers, are standardized for activity by mixing the enzyme with suitable solvents or inert excipients. It is obvious that the use of separate enzymes is somewhat comprehensible. Therefore, another object of this invention is to provide enzymatic mixtures for the production of fructose from starch or a starch-containing raw material, which exhibit a combined activity of oC-amylase-glucose amylase-glucose omerase. Such preparations are standardized according to their (individual activity. Combined enzyme preparations of dd use in the process of the invention should be designed to contain relevant activities within the limits specified in the description of individual enzymes. The resulting solutions of carbohydrates containing fructose can be purified 54 seconds using operations and adventures to oHUoi and other well-known solutions of glevods, i.e., treatment with activated charcoal, ion exchangers with active lines, eeprodialia, and so on. The starch coking process is not harmful to gluco-amylase or ozo-eozyme glitches, for example, psychoactive processing raw material from arthofile starch (known process) .1OOO g potato starch (18%) was mixed with 16OO g water and 1.07 g Tenazy K {O, 13% on the ear starch). The mixture was stirred at a pH of 6.6 to 20 MN chickpeas, higher than 5O to 7 ° C, maintained at 10 minutes, up to BO C for S minutes. kept at this temperature for another 20 minutes. After that, the DE was equal to 18 & . The temperature was lowered to 6 ° C and diluted hydrochloric acid was adjusted to pH 4.2. Then, 1.83 ml of glucose laa (amyloglucosidase L 1OO Meiza) with 1OO EHA / ml activity and body was maintained at the indicated level for 48 hours with continuous mixing. After this period, the DE was equal to 96.7, PRI me R 2, Obtaining a glucose processed raw material with DE-96 from corn starch (a known process), 1OOO g of corn starch (14% depression) was mixed with 172O g of water and 1.12 g of Tenase T (0.13% for dry starch). The process conditions were the same as in example 1, except that the final temperature of 85 ° C was maintained for 35 minutes, DE was 12, Saccharification was carried out as in Example 1 with 1.91 ml of amyloglucosidase L 1OO Miles, At the end The work of DE was equal to 96.1, PRI me R 3, Conversion of processed raw materials with DE-96 examples. 1 and 2 in the fructological glucose syrup (known process), the glucose starting materials with DE equal to 96, of example 1 and 2 were converted by glucose co-isomerase. Conditions and results are shown in Table 1,

Table 1

In Examples 1-3, it can be seen that the production of unrefined (glucose) glucous syrup still requires improvement, and the resultant from liquefied starch, depending on the type of nptmecca, requires 138 hours.

Example 4, Production of 4 uktoznogo yukoz syrup by the simultaneous action of glucose amylase and glucose isomerase.

- Initial DE

Added stabilizers, wt.% For the whole solution: .Mg-SO -TH O

t goce j

- Temperature, С I pH (continuously adjustable with OD NdOH)

Reaction time, hour

The liquefied potato and corn starch of examples 1 and 2 were subjected to the action of the combined nest: 1MY preparation having glucose amypase and glucose isomerase activity.

The enzyme preparation had a standardized content of 5 О ЕГА n 1ООО ЕГИ per gram.

For the conversion of 10OO g of burned out starch substances4 7.0 g of an enzyme preparation was used.

table 2

12

0.2

0.02

65

6.2 72 The total time of the rwakai dd psluchev and uktoevo glyukogyuoge from izgvv n0g, with the help of Eo b shtrovaYuinago saviMaytPi & cieiere preparation composition ao tGOibK & 72 hours Approx 5 53 and g of the offspring (VvazV1 C1 14%) was mixed & in iSOO g in “and O, in g Teyazy J Usveeg 1ts (the waves are the same as in measurement 1. After the SHE DE, bee jsaeeH 18 C) each time to adjust the pH to € ф fovkv standards and so @ in example 1 Vyv to @ srno 3 | 5 g combined with an AIZIMNGO preparation 5O EGA and I.OOO BSH,) d extracting a mixture of extract ari 65 C and pH 6.2 6O hours After this period, the content of fruit was 47, 0% of the weight (ea and h1eg; jyh id0v 6% of the weight of the dry substance) Example 6, Obtaining-1Heauktzno (crushed corn from glucose from shredded maize by glucose isomers {flow 1. 1OOO kg of wobbled maize, devoid of dajpo biiiteftt and zooo icr were heated in a cauldron of General 6 atm for 1 hour. After that, the tank was cooled in a vat, cooled immediately in 6S C, the pH was equal to 6.2 supported QQCTOjaHiEbiM at this level until the end of the process, as well as Teboi ai a. Stabilizers were added in the same amounts as in examples 3 and 4.: 6.5 kg of enzyme was added | ip carat with alpha -amipase activity of 1OOOOO MBE / g, amylog yukozidazuyu activity of 5O EGA and glucoeolio-mymervnuyu activity of 1OOO EGI / g, KbAd HHHpoBaHHaa clestyteriz in, saccharification in isomerization continued 5O hours, riiDcne which I. bsh solution OTF 1ltrovan ffii filterpress. The resulting clear solution contained 755 kg of substances for a total weight of 3551 kg, Frou &

Claims (1)

The continuation of the table 2 Toza contained 316 kg, glucose 344 kg, opigosaccharides 75 kg. The solution for decolorization, decontamination and removal of nitrogenous substances was treated with activated carbon and ion exchange resin, as usual for factory corn syrche, 1 and finally concentrated in a film evaporator to a solids content of 75%. The resulting syrup was red-transparent and contained 32% by weight, glucose 35% by weight, dnOs and gosakharyds 7.7% by weight and 35 psycho 1 zero. EXAMPLE 7 A suspension of corn starch with a content of 44% by weight of dry JBemscTB was kheysterized in Croeer's converter with hydrochloric acid to a DZ equal to 17.5. For conversion into fructose there were 10 kg of this product, but caustic sodium was found pH was maintained at this level, Stabilizers were added according to npss measure 4, and the temperature was maintained at 65 ° C, glucose / ylase (0.25 EGA per 1 g of solids) and glucose isomerase (8 EGI per 1 g of solids) were added After 4S hours, it was determined that the contents were ozg by weight d-Fruko oses 21.2% by weight, D-psicose have, di-and polysaccharides cocTaBjfaioT rest. Claim 1. The method of obtaining fructose, which envisages the saccharification of liquefied starch to glucose in the diet {goatsu goat to fructose, characterized in that, in order to accelerate the process, saccharification of liquefied starch to glucose and isomerization of glucose means the substance. administering an enzyme preparation, glucose glucose amylase and glucose isomerase activity, 2, the method according to claim 2, so that 1 kg of carbohydrates is added to the feedstock & n C pH 5, O-7.5, prvnambust test drugs that have th 5O-500 ydanitsPgddo -: vyon5o at 6O-7O С and pH 6.0-6.5, goat activity and 20OO-2OOOOSource nvformtvts accepted in units of glucose isomerase activity. attention during examination: 3, the Method according to claim 1, about t l and h and s -1, French Patent No. 2086752, y and with the fact that the process is carried out by cl. C 13 K 1/00, 1971, pate. ;;;;o Ш5.; .. and I. ten