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SU604556A1 - Synthetic medium for diluting and freezing animal sperm - Google Patents

Synthetic medium for diluting and freezing animal sperm

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Publication number
SU604556A1
SU604556A1 SU762362962A SU2362962A SU604556A1 SU 604556 A1 SU604556 A1 SU 604556A1 SU 762362962 A SU762362962 A SU 762362962A SU 2362962 A SU2362962 A SU 2362962A SU 604556 A1 SU604556 A1 SU 604556A1
Authority
SU
USSR - Soviet Union
Prior art keywords
lactose
freezing
medium
yolk
semen
Prior art date
Application number
SU762362962A
Other languages
Russian (ru)
Inventor
Евгений Михайлович Платов
Original Assignee
Всесоюзный Ордена Трудового Красного Знамени Научно-Исследовательский Институт Животноводства
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Всесоюзный Ордена Трудового Красного Знамени Научно-Исследовательский Институт Животноводства filed Critical Всесоюзный Ордена Трудового Красного Знамени Научно-Исследовательский Институт Животноводства
Priority to SU762362962A priority Critical patent/SU604556A1/en
Application granted granted Critical
Publication of SU604556A1 publication Critical patent/SU604556A1/en

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Claims (1)

Изобретение относитс  к области искусственного осеменени  сельскохоз йственных животных, в частности к синтетическим средам дл  разбавлени  и замораживани  спермы л ивотных, пр-еимущественно бара ов. Известна синтетическа  лактозо-желточна  среда дл  спермы животных, например баранов , состо ща  из изотонического раствора лактозы, желтка куриного  йца и глицерина 1. Однако лактозо-желточна  среда не обладает достаточным холодозащитньгм действием , вследствие чего сперма, разбавленна  и замороженна  в этой среде, имеет низкие подвижность и оплодотвор ющую способность после оттаивани . Целью изобретени   вл етс  создание среды , содержащей в качестве основы дистиллированную воду, желток куриного  йца, лактозу , а также глицерин, котора  обладала бы высокими холодозащитными свойствами при глубоком замораживании опер.мы (сохранение биологической полноценности сперматозоидов , повышение подвижности, оплодотвор юи;ей способности).. Дл  достижени  указанной цели известна  ла-ктозо-желточна .. среда дополнительно содержит в качестве холодозащитного агента полисахарид - гуммиарабик при следующем соотнои ении ингредиентов, вес. %: Лактоза7,5-9,а Желток куриного  йца10,0-20,0 Глицерин2,5-5,0 Гуммиарабик0,5-5,0 Вода дистиллированна До 100 Оптимальный вариант -предлолсеиной среды содержит ингредиенты в следующих соотношени х , вес.- %: Лактоза8,67 Л елток куриного  йца14,46 Глицерин3,17 Гуммиарабик1,44 Иредложенна  среда дл  разбавлени  и замораживани  спермы (например, баранов) обладает высокими .холодозащитными свойства .ми, в результате чего оплодотвор юща  способность отта нной после замораживани  спермы повыщаетс  вдвое. Формула изобретени  Синтетическа  среда дл  разбавлени  и замораживани  спермы животных, преимущественно баранов, содержаща  лактозу, желток куриного  йца, глицерин и воду, отличающа с  тем, что, с целью повыщени  холодозащитных .свойств среды при глубоком за мораживании спермы, она дополнительно содержит гуммиарабик при следующем соотнощении исходных ингредиентов, вес. %:This invention relates to the field of artificial insemination of farm animals, in particular, synthetic media for diluting and freezing semen from animal husbandry, especially bars. The known synthetic lactose-yolk medium for sperm of animals, for example rams, consisting of an isotonic solution of lactose, egg yolk and glycerin 1. However, the lactose-yolk medium does not have sufficient cold-protective effect, as a result of which sperm diluted and frozen in this medium has low mobility and fertilizing ability after thawing. The aim of the invention is to create an environment containing distilled water as a base, chicken egg yolk, lactose, and also glycerol, which would have high cold-protective properties during deep freezing of the operm (preservation of the biological usefulness of spermatozoa, increased mobility, fertilizer; ) .. To achieve this goal, la-who-yolk is known .. the medium additionally contains as a cold-protective agent a polysaccharide - gum arabic at the following ratio of ingredients in weight %: Lactose 7.5–9, and Chicken Egg Yolk 10.0–20.0 Glycerin 2.5–5.0 Gum arabic 0.5–5.0 Distilled water Up to 100 The best option is predolol-ester medium containing ingredients in the following ratios, wt. -%: Lactose 8.67 L of chicken egg14.46 Glycerol3.17 Gum arabic1.44 An appropriate medium for diluting and freezing semen (for example, rams) has a high cold-protective properties, resulting in a fertilizing ability detected after freezing of sperm, which results in an increase in the semen content of the semen after it is frozen, so that the fertilizing capacity detected after freezing of semen will increase, and as a result, the fertilizing capacity of the semen becomes frozen. twice. Synthetic medium for diluting and freezing the sperm of animals, mainly sheep, containing lactose, chicken egg yolk, glycerin and water, which also contains gum arabic in the following to increase the cold-protective properties of the medium during the freezing of sperm the ratio of the original ingredients, weight. %: ЛактозаLactose Желток «у.риного  йцаYolk "u.rino ytsa ГлицеринGlycerol ГуммиарабикGum arabic Вода ди.стилли1р01ванна Water d.styli1r01vanna Источники информации, прйй тйе )во siiHMdkHe  ри эМЙёртизе ii. Платов Ё. М. Замораживание семени барана в лактозб-желточно-этиленглйколевой среде. - Овцёво1дство, 1965, № §, с. 11 - 12.Sources of information, see) in siiHMdkHe ri eMYortize ii. Platov Y.M. Freezing of sheep's seed in lactose-yolk-ethylene glycol medium. - Hermitage, 1965, No. §, p. 11-12.
SU762362962A 1976-05-25 1976-05-25 Synthetic medium for diluting and freezing animal sperm SU604556A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SU762362962A SU604556A1 (en) 1976-05-25 1976-05-25 Synthetic medium for diluting and freezing animal sperm

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU762362962A SU604556A1 (en) 1976-05-25 1976-05-25 Synthetic medium for diluting and freezing animal sperm

Publications (1)

Publication Number Publication Date
SU604556A1 true SU604556A1 (en) 1978-04-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
SU762362962A SU604556A1 (en) 1976-05-25 1976-05-25 Synthetic medium for diluting and freezing animal sperm

Country Status (1)

Country Link
SU (1) SU604556A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4454227A (en) * 1980-12-20 1984-06-12 Hoechst Aktiengesellschaft Method for cultivating insect cells
WO1997014785A3 (en) * 1995-10-19 1997-09-12 Advanced Reproduction Technolo Methods and compositions to improve germ cell and embryo survival and function

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4454227A (en) * 1980-12-20 1984-06-12 Hoechst Aktiengesellschaft Method for cultivating insect cells
WO1997014785A3 (en) * 1995-10-19 1997-09-12 Advanced Reproduction Technolo Methods and compositions to improve germ cell and embryo survival and function
US6140121A (en) * 1995-10-19 2000-10-31 Advanced Reproduction Technologies, Inc. Methods and compositions to improve germ cell and embryo survival and function
US6593309B2 (en) 1995-10-19 2003-07-15 Bio-Origyn Llc Methods and compositions to improve germ cell and embryo survival and function
US7838509B2 (en) 1995-10-19 2010-11-23 Bio-Origyn Llc Methods and compositions to improve germ cell and embryo survival and function

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