SU587398A1 - Method of determining specific antibodies - Google Patents

Description

one

This invention relates to immunopogy and can be used to study immunity in case of bacterial and viral infections, as well as in vaccination.

A method is known for determining specific antibodies belonging to different immunoglobulin clauses by sorbpy from a sample of immunoglobulins of the test class using an immunosorbent prepared on the basis of antiserum to this class with a sample of these immunoglobulins and definition of specific patterns that have been determined by the specific characteristics of the immunoglobulins that have been studied by specific immunoglobulins.

However, this method is time consuming and requires a large number of operations. In order to simplify the method, the associated immunoglobulins are removed from the test liquid by means of an immunosorbent and the target substance is determined directly in the liquid. 20

Example. Antibodies against antigens from SH "Sonnei" (oxygene) (LL) in the sera of patients with Sonne dysentery are determined. Dp of this from superhorst adsorbirullet all imm / but globulins of classes 3 J and 3 q, M. And dsrrbtsiyu ammu

Footblobulin classes ZdRAZ3E are not conducted because the content of these immunoglobulins in the serum is 10 10 O-O times less than 3 A, and also because the antibodies of the class ZEE are not detected in the reaction of haggaglutination used in this study to determine specific antibodies. Thus, the immunoglobulins ZaO and Zd can not have any significant effect on the determination results.

Claims (2)

For the adsorption of ZaO and ZaM from serum, the immunosorbent was previously prepared. For this purpose, the amounts of undiluted monospecific sera against ZoM (titer 1: 1 o) and against Za Q (titer l: 15j are mixed. To the mixture of whey, with stirring, add 5% glutaraldehyde solution (at the rate of 3 volume of this solution per 10 volumes of serum) is left for 3 hours at room temperature.After 3 hours the resulting gel is thoroughly homogenized 7 into homoteniters and washed three times with 0.15 M sodium chloride solution, buffered 0.01 M phosphate 1 st buffer, pH 7, 2. Then, in each of the studied sera, an immunosorbent, carefully precipitated with a homogenizer, previously precipitated with a chain of fugging, is added. 1.5 ml of serum is taken from immunosorbent prepared from 1 ml of a mixture of monospecific anti-Oq Q and ZDM sera taken in a 1: 1 ratio. Remain in a thermostat at 37 ° C for 1 hour and then overnight in a refrigerator, Mr. Morning immunosorbent s; are centrifuged and two more similar adsorption are carried out. In the second I and third adsorption of 1.5 m, syrups each time take immunosorbent in an amount with a 0.5 ml mixture of monospecific serums. After three adsorption, the immunoglobulins oQH 3 o M are absent in the test sites, as evidenced by the reaction of immunodiffusion in agar according to the method of Mancinl. Then, in the adsorption of the tested svbrbtkah determined using the reaction of nesnoy hemagglutination of antibodies (class G. A) against Sh. Sonnet. Lipopolysaccharide is used as an antigen. ZNVott x, and the concentration of detectable immunoglobulin in the sample subjected to adsorption, is almost equal to its concentration in the original one material. This leads to a higher sensitivity of the method compared to the existing one, all of which leads to an observation of the laboriousness of the method and the possibility of determining it immediately in a large number of samples. Claims; The method of determining specific antibodies belonging to different immunoglobulin kpacs by sorption, is different with the fact that, in order to simplify the method, co-immunoglobulins are removed from the test liquid using immunosorbent and the target product is determined directly in the liquid. Sources of information taken into account during the examination: 1. EMIS,. 1.1951, s. 125-126.