SU580216A1 - Bac.thuringiensis var. galleriae k-4-75 strain - Google Patents
Bac.thuringiensis var. galleriae k-4-75 strainInfo
- Publication number
- SU580216A1 SU580216A1 SU7502319249A SU2319249A SU580216A1 SU 580216 A1 SU580216 A1 SU 580216A1 SU 7502319249 A SU7502319249 A SU 7502319249A SU 2319249 A SU2319249 A SU 2319249A SU 580216 A1 SU580216 A1 SU 580216A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- strain
- galleriae
- bac
- thuringiensis var
- thuringiensis
- Prior art date
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- Agricultural Chemicals And Associated Chemicals (AREA)
Description
Изобретение относитс к микробиологической промышленности, в частности к производству энтобактерина, примен емого дл уничтожени насекомых-вредителей сельскохоз йственных культур.The invention relates to the microbiological industry, in particular to the production of entobacterin, used to kill insect pests of agricultural crops.
Производство энтобактерина основано на штаммах Вас. thuringiensis, синтезируюш,их равные количества спор и кристаллов. Эти свойства микроорганизмов, обеспечивающие высокую эффективность препарата, нестабильны и часто мен ютс в процессе производства энтобактерина 1.Production of entobacterin is based on strains of Vas. thuringiensis, synthesized, their equal amounts of spores and crystals. These properties of microorganisms, which provide high efficacy of the drug, are unstable and often change during the production of entobacterin 1.
В насто ш,ее врем в микробиологической промышленности дл производства энтобактерина-3 используют штамм Вас. thuringiensis var galleriae 69/6. Штамм 69/6 выделен из гусениц большой пчелиной огневки и хранитс в коллекции ВНИИзащиты растений 2.At present, her time in the microbiological industry for the production of entobacterin-3 is used strain You. thuringiensis var galleriae 69/6. Strain 69/6 is isolated from the caterpillars of the large bee fire and is stored in the VNII Plant Protection collection 2.
Однако штамм 69/6 вл етс чувствительным к вирулентным фагам и, кроме того, в процессе глубинного культивировани на жидких питательных средах быстро тер ет вирулентность и сохранность.However, strain 69/6 is susceptible to virulent phages and, moreover, in the process of deep cultivation on liquid nutrient media, it quickly loses its virulence and integrity.
Целью изобретени вл етс получение такого штамма Вас. thuringiensis, который отвечает всем требовани м промышленного способа производства энтомопатогенного бактериального препарата энтобактерина-3, штамма , обладающего фазоустойчивостью, хорошей сохранностью и высокой вирулентностью.The aim of the invention is to obtain such a strain of Bac. thuringiensis, which meets all the requirements of the industrial method for the production of the entomopathogenic bacterial preparation entobacterin-3, a strain with phase stability, good preservation and high virulence.
без утраты этих признаков в услови х глубинного культивировани на жидких питательных средах.without losing these traits under conditions of deep cultivation on liquid nutrient media.
Штамм Вас. thuringiensis К4-75, отвечающий указанным требовани м, выделен авторами из исходного дикого лизогенного штамма 69/6 методом направленной селекции с включением таких этапов, как глубинное культивирование в присутствии экзогенных вирулентных фагов , /FlA, /Р2А, VF3A, VF4A, XF5A, VF5B, пассажи на обогащенном аминокислотами и микроэлементами агаре и иропуск через насекомых-вредителей , вл ющихс тест-объектами к данному серотипу.Strain you. thuringiensis K4-75, which meets these requirements, was isolated by the authors from the original wild lysogenic strain 69/6 using the method of directional selection with the inclusion of such stages as deep cultivation in the presence of exogenous virulent phages, / FlA, / P2A, VF3A, VF4A, XF5A, VF5B , passages on agar and enriched with amino acids and microelements and iropus through insect pests, which are test objects for this serotype.
Штамм К4-75 обладает следуюпхими свойствами .Strain K4-75 has the following properties.
Клетки пр мые, палочковидные, размером 2,0X5,0 мкм. Малоподвижны, встречаютс в виде коротких и длинных цепей. Перитрихи грамположительны. В период спорообразовани образуют овальные споры, слабо прокрашивающиес фуксином, и крупные в виде тетраэдров , энтомоцидные белковые кристаллы, хорошо прокрашивающиес 1%-ным раствором амидо черным и кумаси в 7%-ной уксусной кислоте.The cells are straight, rod-shaped, 2.0X5.0 microns in size. Low mobility, found in the form of short and long chains. Peritrichs are gram positive. During the sporulation period, oval spores are formed, slightly staining with magenta, and large in the form of tetrahedrons, entomocidal protein crystals, well staining with 1% amido black solution and coomasi in 7% acetic acid.
Органическа среда дл размножени в услови х поверхностного роста, СПА 5%, дл глубинного культивировани дрожжеполисаOrganic medium for reproduction under surface growth conditions, SPA 5%, for deep cultivation of yeast polis
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7502319249A SU580216A1 (en) | 1975-11-21 | 1975-11-21 | Bac.thuringiensis var. galleriae k-4-75 strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU7502319249A SU580216A1 (en) | 1975-11-21 | 1975-11-21 | Bac.thuringiensis var. galleriae k-4-75 strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU580216A1 true SU580216A1 (en) | 1977-11-15 |
Family
ID=20647206
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU7502319249A SU580216A1 (en) | 1975-11-21 | 1975-11-21 | Bac.thuringiensis var. galleriae k-4-75 strain |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU580216A1 (en) |
-
1975
- 1975-11-21 SU SU7502319249A patent/SU580216A1/en active
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