SU481289A1 - The way organ conservation - Google Patents

Description

I oxygen into the cell, its consumption in the cell itself at an adequate level of oxidative processes, then through the membrane elimination of excess oxygen and carbon dioxide, and with them to the other. GIH products of medabolism in the extracellular space and their free exit from the body. Cell breathing is provided by the delivery of oxygen without the aid of liquid transport, unlike all means of liquid perfusion. Organ Perfu; It is continuously gassed for the duration of its conservation. The excess gas escapes through the capsular vessels to the surface I of the liquid in which the organ is submerged in the sup - buoy. This eliminates the possibility of drying the body. During preservation, the temperature in the refrigerator compartment is maintained; c at the level of 6-4 C. This mode ensures the prolongation of the periods of the life-support of an isolated organ up to 10-1 days. To preserve organs for longer periods of time, an isotonic solution is replaced by a red crystal protector for a long time, and then the temperature in the chamber is gradually reduced to minus 8 - minus and lower temperatures while simultaneously connecting the metal coil to the gas pipeline {for adequate cooling of gas injected into the organ ). In this mode, a uniform deviation of the temperature of the organ occurs both from the outside and from the inside. The preliminary cleaning of an organ from the blood by a cryoprotector, as well as the replacement of an isotonic solution in which the organ is immersed with a cryprotectant, reliably protects the organ, both outside and inside, from the formation of ice crystals. The viability of the preserved organ according to the method of transorgan gas, peroxides is controlled (by the example of the kidney) by heterotopic replantation of the same animal from which the organ was taken, followed by removal of the second intact kidney. The subject of the inventions. A method of preserving organs by organ oxygen perfusion at low and low temperatures, which means that, in order to prolong the periods of survival of isolated organs, moistened oxygen under pressure is forced into the organ through all communications of the organ.