SU460292A1 - Method for saccharification of starchy raw materials with enzyme preparations - Google Patents

Description

The invention relates to the field of processing starch raw materials, and in particular to methods for saccharifying it with enzyme preparations in the production of alcohol. There is a method of saccharification of starchy raw materials with enzyme preparations. The known method does not provide α-complete saccharification, namely a mixture of malt or microorganism culture, used in the process of saccharification of starchy raw materials as sources of amylolytic enzymes, hydrolyzes starch incompletely to form non-fermentable phosphodextrins, and the presence of phosphorus in dextrins increases their resistance to cleavage. In order to more fully saccharify the starchy raw material in accordance with the proposed method, the saccharification process is carried out with phosphatase in the amount of 200-500 U FA / g of starch. The proposed method is carried out as follows. The phosphatase preparation with the activity of ФА89000 u / g is set simultaneously with the malt at the rate of 200-500 u / g of starch and the saccharification of starchy raw material is carried out at 55-58 ° C, then the mash is clarified by precipitation and fermented. For the preparation of the active drug, Aspergillus awamori 22 is used and the medium is selected for the implementation of the phosphatase synthesis biosynthesis method using the deep-growing producer, soybean from the following components,%: Starch6 ZCl0.05 MgSO40.05 KH4NO30.43 10% StSO6,001 KCl0.05 MgSO40.05 KH4NO30,41 10% stretching 5. The medium provides the largest culture liquid, from which the apparatus is separated by precipitation with ethyl alcohol.

The drug has an activity on FOSfatase 70000-89000 IU / g.

The introduction of such a drug into the saccharifying mixture helps to increase the ethanol yield by 0.6-2.0% due to the role of phosphatase in the Starch hydrolysis process.

Phosphatase destroys the ester linkage between the glucose residue and phosphorus, which prevents the dextrins from being cleaved by amylolite-digesting enzymes, resulting in an increase in the amount of fermented substances in the mash and, consequently, an increase in ethanol output from starchy raw materials.

With the introduction of 200-500 edfa / g of starch, malt consumption decreases to 50-70%.

Indicators of -block congestion at 30-50% malt substitution with phosphatase enzyme preparation are given in the table.

Subject invention

The method of saccharification and starchy raw materials with enzyme preparations, characterized in that, for the purpose of more complete oxidation, the process is carried out with phosphotase in an amount of 250-500 g FA / g starch.