SU390442A1 - - Google Patents

Description

DEVICE FOR RECORDING PHOSPHORESCENCE OF BIOLOGICAL OBJECTS

one

The invention relates to a technique for studying the viability of biological objects in order to investigate their physiological state.

A known device for recording phosphorescence of biological objects, comprising a light source, a light filter system, a working camera, a time unit for controlling the operation of the gates, a photoelectric receiver and a recording device.

This device has the disadvantages of having to place the plant in a dark chamber, which violates the natural course of photophysiological processes, as well as in violation of the viability of the leaves of the plant.

In addition, the known device does not have devices for adjusting the irradiance spectra and recording the phosphorescence.

In order to register the phosphorescence of plant objects, for example, the leaves of plants, without disturbing their viability, as well as the possibility of research in various growing conditions of plants in the proposed device, the working chamber is made of two parts detachable in the plane of perpendicular optical axis light source, and in the other - a photoelectric receiver.

In order to ensure light insulation and to avoid damage to the objects under study, in the device described, the separating parts of the working chamber are equipped with inserts made of elastic materials of different hardness.

In order to reduce the time spent on analysis, the device is equipped with a connector mechanism for parts of the working chamber, which simultaneously fixes and evenly presses the object under investigation.

FIG. 1 is shown for a functional block scheme of the device proposed; FIG. 2 - kinematic scheme of the optomechanical unit.

The main elements of the device are the radiation source /, heat-shielding filter 2, condenser 3, neutral filter 4, system of replaceable excitation filters 5, photo shutter 6 of excitation, working chamber 7 for the object, photo lock 8 of phosphorescence, system of replaceable filters 9 of recording phosphorescence, photon receiver 10, unit 11 of the object irradiation time, trigger 12, unit 13 of phosphorescence recording time, photocurrent amplifier 14 with pointer 15, automatic recorder 16 of phosphorescence intensity and time, power supply unit 17

The optical-mechanical unit of the device contains a light source 1, a reflector 18, a heat-shielding light filter 2, which protects the lens optics from infrared radiation, mounted in a housing 19 rigidly connected to the movable part 20.

To attenuate the light flux, a replaceable neutral light filter is installed behind the lens, and for irradiating an object with different monochromatic parts of the spectrum there is a set of interference light filters 21 placed in cassette 22. A photo gateway 6, activated by an electromagnet 2, blocks the light flux after the end of the irradiation time.

To install the object of study, the unit has a working chamber formed by washers 24 and 25 of elastic material, which are detachable parts, driven by a pair of helical gears 26, 27, rails 28 and a handle 29. Shutter 8, actuated by an electromagnet 30 serves to cut off the photodetector 10 from the light flux of the light source at the time of irradiation. To isolate the spectrum of phosphorescence, there are interference light filters 31 installed in cassette 32.

The device works as follows.

By turning the knob upward with the aid of a gear pair 26, 27 and a slat 28, the upper part of the device rises up, forming a gap between the washers 24 and 25. The object to be examined is placed in this gap. By turning the handle down, the excitation unit is lowered, thereby making the object tightly clamping and strictly fixing it in the working chamber. Further operations are performed using the measuring and controlling unit: set the time of irradiation and recording by the setting devices 11 and 13 and start the device using the device 12, which

the shutter 6 opens the set time. Light from a source / through a system of light filters and a condenser hits the object. After a predetermined time, shutter 6 closes, and after a minimum pause of 0.1-0.2 seconds, shutter 8 opens. The object's phosphorescence through filter 9 acts on the photodetector W. The photocurrent is amplified by the recorder 16 during the time that shutter 8 is open, displaying its nature recession in time.

Subject invention

Claims (3)

1. An instrument for recording phosphorescence of biological objects, comprising a light source, a light filter system, a working chamber, a time unit for controlling the operation of the gates, a photoelectric receiver and a recording device, characterized in that, in order to record the phosphorescence of plant objects, for example plant leaves, without disturbing their livelihoods, as well as opportunities. studies in different conditions of plant growth, the working chamber is made of two parts detachable in the plane perpendicular to the optical axis, one of which contains a light source and the other a photoelectric receiver. 2. The apparatus according to claim 1, characterized in that, in order to provide light insulation and avoid damage to the objects under study, the separating parts of the working chamber are provided with inserts made of elastic materials of different hardness. 3. The device for 1pp. 1 and 2, characterized in that, in order to reduce the time spent on the analysis, it is equipped with a mechanism for separating the parts of the working chamber, which at the same time provides for the fi xing and uniform pressing of the object under study. I-sh : W I-0 nineteen 2