SU1691391A1 - Method of fibroblast cultivation - Google Patents
Method of fibroblast cultivation Download PDFInfo
- Publication number
- SU1691391A1 SU1691391A1 SU843787515A SU3787515A SU1691391A1 SU 1691391 A1 SU1691391 A1 SU 1691391A1 SU 843787515 A SU843787515 A SU 843787515A SU 3787515 A SU3787515 A SU 3787515A SU 1691391 A1 SU1691391 A1 SU 1691391A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- substrate
- cells
- simplify
- yield
- fibroblast
- Prior art date
Links
- 210000002950 fibroblast Anatomy 0.000 title claims abstract description 8
- 238000000034 method Methods 0.000 title claims abstract description 6
- 210000004027 cell Anatomy 0.000 claims abstract description 9
- 239000000758 substrate Substances 0.000 claims abstract description 8
- 229920006173 natural rubber latex Polymers 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Изобретение относитс к биолоши v биотехнологии и может Ьыть использовано рп культивировани фибробластов. Целью изобретени вл етс упрощение способа культивировани фибробластов и повышение выхода жизнеспособных клеток. Это достигаетс использованием подложки из латекса натурального каучука, а также тем, что съем выросших клеток осуществл ют путем раст гивани подложки. 1 табл.The invention relates to bioloshes v biotechnology and can use rp fibroblast cultivation. The aim of the invention is to simplify the method of culturing fibroblasts and increasing the yield of viable cells. This is achieved by using a natural rubber latex substrate, as well as by removing grown cells by stretching the substrate. 1 tab.
Description
Изобретение относитс к биологии и биотехнологии и может быть использовано дл культивировани клеток, в частности, фибробластов .The invention relates to biology and biotechnology and can be used to cultivate cells, in particular fibroblasts.
Цель изобретени -упрощение способа культивировани фибробластов и повышение выхода жизнеспособных клеток.The purpose of the invention is to simplify the method of culturing fibroblasts and increasing the yield of viable cells.
Способ осуществл ют следующим образом .The method is carried out as follows.
Дл изготовлени подложки 2 мл жидкого латекса натурального каучука наливают на дно чашки Петри с площадью дна около 12 см2. После коагул ции латекса на воздухе и образовани пленки ее поверхность промывают бидистиллированной водой,стерилизуют 70%-ным раствором этанола в течение не менее 30 мин и ополаскивают питательной средой, например средой Игла . Затем на поверхность подложки нанос т суспензию клеток линии ВНК-21 в среде Игла без сыворотки в концентрации 10 тыс.To prepare the substrate, 2 ml of liquid natural rubber latex is poured onto the bottom of a petri dish with a bottom area of about 12 cm2. After coagulation of the latex in air and film formation, its surface is washed with bidistilled water, sterilized with 70% ethanol solution for at least 30 minutes, and rinsed with a nutrient medium, such as Eagle's medium. Then, a suspension of VNK-21 cells in serum needle at a concentration of 10 thousand was applied to the surface of the substrate.
клеток на 1 см подложки. После инкубации в известных услови х выросшие клетки снимают с подложки, слегка раст гива ее пинцетом и ополаскива в небольшом количестве среды Игла.cells per 1 cm substrate. After incubation under known conditions, grown cells are removed from the substrate, slightly stretching it with tweezers and rinsing in a small amount of Eagle's medium.
Результаты выращивани фибробластов в сравнении с известным приведены в таблице.The results of growing fibroblasts in comparison with the known are given in the table.
Таким образом, реализаци изобретени позвол ет повысить выход жизнеспособных клеток фибробластов на 30-35% и упростить способ культивировани ь-х.Thus, the implementation of the invention makes it possible to increase the yield of viable fibroblast cells by 30-35% and simplify the culture method.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU843787515A SU1691391A1 (en) | 1984-09-05 | 1984-09-05 | Method of fibroblast cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU843787515A SU1691391A1 (en) | 1984-09-05 | 1984-09-05 | Method of fibroblast cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1691391A1 true SU1691391A1 (en) | 1991-11-15 |
Family
ID=21137312
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU843787515A SU1691391A1 (en) | 1984-09-05 | 1984-09-05 | Method of fibroblast cultivation |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1691391A1 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007114726A1 (en) | 2006-04-06 | 2007-10-11 | Boris Karpovich Gavrilyuk | Monolayer wound coating and a method for the production thereof |
| RU2320720C2 (en) * | 2006-01-25 | 2008-03-27 | Елена Викторовна Парфенова | Method for culturing fibroblasts for substitution therapy |
| WO2012115658A1 (en) * | 2011-02-25 | 2012-08-30 | Empire Technology Development Llc | Dynamically alterable cell support |
-
1984
- 1984-09-05 SU SU843787515A patent/SU1691391A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Авторское свидетельство СССР Ns 1251528, кл. С 12 N 5/00, 1984. * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2320720C2 (en) * | 2006-01-25 | 2008-03-27 | Елена Викторовна Парфенова | Method for culturing fibroblasts for substitution therapy |
| WO2007114726A1 (en) | 2006-04-06 | 2007-10-11 | Boris Karpovich Gavrilyuk | Monolayer wound coating and a method for the production thereof |
| WO2012115658A1 (en) * | 2011-02-25 | 2012-08-30 | Empire Technology Development Llc | Dynamically alterable cell support |
| US9388379B2 (en) | 2011-02-25 | 2016-07-12 | Empire Technology Development Llc | Dynamically alterable cell support |
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