SU1656417A1 - Method for determining 2-(n-aminobenzenesulfamido)-5-ethyl-1,3,4- thiadiazol-sodium - Google Patents

Abstract

This invention relates to analytical chemistry, in particular, to methods for determining ethasol sodium used in medical practice. The purpose of the invention is to increase the sensitivity of the method. The invention consists in preparing a solution of the analyte in dioxane with a concentration of 1.6-4.8 µg / ml. introduction of benzoylhydrazone 2.6-dibromo-1,4-benzoquinone imine to the solution to a concentration of 0.012% and photometry of the solution at a wavelength of 503 nm 4 or 2 gab. (Ls

Description

The invention relates to analytical chemistry, and specifically to methods for the quantitative determination of 2- (p-amino-benzenesulfamido) -5-ethyl-1,3, 4-thiadiazole "-sodium (ethazol-gri) used in medical practice as an antibacterial facilities.

The purpose of the invention is to increase the sensitivity of the determination method.

FIG. 1 shows the dependence of the optical density D of an analytical solution on the wavelength I (curve 1); in fig.

2 - dependence of the optical density of the analytical solution on time using dioxane (curve 2), acetone (curve 3) and ethanol (curve 4); in fig. 3 - dependence of the optical density of the analytical solution on the time when using 2 ml of 0.1% reagent solution per 25 ml of analytical solution (curve 5),

3 ml / 25 ml (curve 6), 4 ml / 25 ml (curve 7) and 5 ml / 25 ml (curve 8) in FIG. 4 - the dependence of the coefficient E 1cm absorption from

concentrations are determined on the substance (curve 9).

The method is carried out as follows.

The quantitative determination of 2- (p-aminobenzenesulfamido) -5-ethyl-1,3,4-sodium diazole-sodium in a substance is carried out as follows. The exact weight of the analyte in the range of 0.020-0.030 g is dissolved in 1 ml of water in a 50 ml volumetric flask and diluted with dioxane to the mark. 2.5 ml of the resulting solution is placed in a 25 ml volumetric flask and diluted with dioxane to the mark, 2 ml of the resulting dilution is placed in a 25 ml volumetric flask. 3 ml of a 0.1% solution of benzoyl hydrazone 2,6-dibromo-1,4-benzoquinone imine in dioxane are added and adjusted to the mark with the same solvent. A parallel experiment was carried out with 2 ml of a 0.004% solution of etazol-natrich (standard) and control. The optical density of the colored solutions is measured by the background.

O (L

about

J

Vj

control using a spectrophotometer at 503 nm in cuvettes with a layer thickness of 1 cm. The concentration is calculated by the formula

P-1,42 D-p

where D is the optical density of the test solution;

Do is the optical density of the standard solution;

p - weight, g;

1.42 is a conversion factor taking into account the dilution and concentration of the standard solution.

The results of quantitative determination of 2- (p-aminobenzenesulfamido) -5-ethyl-1,3,4-thiodiazole-sodium in the substance are listed in the table. one.

The results of the analysis of injection solutions of factory manufacture are given in table 2.

Claims (1)

Invention Formula The method for determining 2- (p-aminobenzenesulfamido) -5-ethyl-1,3,4-thiadiazole-sodium-, which consists in dissolving the sample, injecting a color reagent into the solution and photometrically the resulting solution at room temperature, which differs by that. in order to increase the sensitivity of the method, the sample is dissolved in dioxane, the solution is diluted with dioxane until the concentration of the analyte is 1.6-4.8 µg / ml; color reagent benzoylhydrazone 2,6-dibromo-1,4-benzoquinonimine is added to it to a concentration of 0.012%, and photometric is measured at a wavelength of 503 nm. Note x average content; Sr-dispersion St-confidence interval. Table 1 Table 2 at, 08 500 & 0 Ln Fig 1 D 0.8 D 0.8 30vo tw FIG. 2 zo 59 "L / ag.J S1sn 11-Ю3 go w O0,10.20.30, C0.5, FIG. four