SU1523045A3 - Method of producing preparation for curing wounds - Google Patents

Abstract

The topical pharmaceutical formulations contain, uniformly dispersed in a pharmacologically compatible vehicle, a combination of active substances with 0.2 to 10 per cent by weight of an ionisable mixture of pharmacologically compatible Ca<2+> and K<+> salts in the Ca<2+> : K<+> molar ratio of 1:3 to 4:1. Formulations in the form of dry gels or water-containing gels are preferred. In the case of water-containing formulations, the concentration based on the water content is 20 to 100 mM Ca<2+> ions and 25 to 60 mM K<+> ions. The formulations surprisingly promote wound granulation and epithelisation. Also described are processes for the preparation of the formulations according to the invention.

Description

The invention relates to the chemical and pharmaceutical industry and concerns a method for preparing a wound healing agent.

The aim of the invention is to improve wound healing due to the homogeneous distribution of the active substance.

The invention is illustrated by the following rules.

Example 1.94 g of purified water is heated to 70 ° C and mixed with 0.3 g of potassium chloride (40 mmol of potassium ions) and 0.44 g of calcium chloride (30 mmol of calcium ions). After adding 0.2 g of propyl paraben in the resulting solution, it is dispersed 5 g of methyloxystilcellulose. After stirring, cool to room temperature. After cooling, a highly viscous gel containing air bubbles with a viscosity of 90 is obtained, which can be used directly to treat wounds. The analysis shows that the active substance is evenly distributed in the gel.

Example 2. The procedure was carried out as in Example 1, but instead of propyl paraben, 0.2 g of methyl paraben was used. After cooling, a highly viscous gel containing 90 air-bubbles with a viscosity of 150 Pa-s is obtained, which can be used directly for the treatment of wounds. The analysis shows that the active substance is evenly distributed in the gel.

Example 3. 40 g of purified water are heated to 80 ° C and mixed with 0.3 g of potassium chloride (40 mmol of potassium ions) and 0.44 g of calcium chloride (30 mmol of calcium ions).

SL

to

:about

4: SL

cm

After adding 0.18 g of methylparaben-J1, 0.02 g of propyl paraben in the resulting solution is dispersed 4.5 g of methyloxyethylcellulose. Then 54.56 g of cold purified water are added and, with stirring, cooled to room temperature. After cooling, an air-containing gel with a viscosity of 50 Pa.s is obtained, which can be used directly to treat wounds. Analysis shows. that the active substance is evenly distributed in the gel.

Example 4. 55.06 g of purified water is heated to 60 ° C and mixed with 0.3 g of potassium chloride (40 mmol of potassium ions) and g of calcium chloride (40 mmol of calcium ions). After adding 0.2 g of ethyl paraben in the resulting solution is dispersed 4 g of methyloxyethylcellulose. Then, 40 g of cold purified water is added and, by stirring, 5 is cooled to room temperature. After cooling, a gel containing air bubbles with a viscosity of 40 Pa-s is obtained, which can be used directly to treat wounds. Analysis shows that the active substance is evenly distributed in the gel,

Example 5. 88 g of purified water are heated to BOC and mixed with 0.3 g of potassium chloride (40 mmol of potassium ions) and 0.44 g of calcium chloride (30 mmol of calcium ions). After adding 0, 2 g of propyl paraben, 1 , 8 g methylparabate and 2.8 g sorbitol in the form of a 70% aqueous solution in the resulting solution are dispersed 4 g of methyloxyethylcellulose. Then, stirring, cooled to room temperature. After cooling, a gel containing air bubbles with a viscosity of 35 Pa-s is obtained, which can be used directly to treat wounds. The analysis shows that the active substance is evenly distributed in the gel.

Example 6. The procedure is carried out in analogy to Example 5, but using 70% N's aqueous solution containing a) 2 g of ethyl paraben and 2.8 g of sorbitol, b). 2 g. methyl paraben and g sorbitol or c) 2 g propyl paraben and 2.8 g sorbitol

After removal, in all cases a) c) a gel containing air bubbles with a viscosity equal to

5 0 5

Q

five

50, which can be used directly to treat wounds. The analysis shows that the active substance is evenly distributed in the gel a) - c).

Example 7 Carried out as in Example 5, however, 40 mmol of potassium and 30 mmol of calcium ions are used as a mixture.

a) potassium and calcium acetates,

b) potassium and calcium gluconates,

c) potassium iodide and calcium iodide as hexahydrate,

d) potassium and calcium lactates,

e) potassium and calcium pantogenates,

e) primary potassium phosphate and drip

CII,

g) tertiary potassium and calcium phosphates,

h) potassium and calcium sugars,. i) salicylates of potassium and calcium,

a) in the form of the corresponding salts of penicillin.

In all cases, an agent with the active substance uniformly distributed in it is obtained.

Example 8 The skin of the back of a guinea pig is divided to the fascia and a ring of Teflon with a diameter of 21 mm is sewn into this wound in order to prevent epithelial wound closure. The fascia of the back muscles, which at the time of the operation is free of granulation tissue, are applied with the preparations of examples 1-5, 6a-6b, 7a-7k, as well as the prototype (gel based on polyacrylamide and gelatin in a 1: 1 ratio, on which 0.9% sodium chloride is applied as the active substance, the trade product is Gel).

The amount formed by using the means of examples 1-7 of granulation tissue is determined as a percentage of the amount of fabric formed by using the known means. The following results were obtained: the means of examples 1-5-180, 190 and 195%, respectively, a, examples 6a-6b, 7a-7k - 195, 192, 195., 193, 195, 190, 198, 188 ,, 190, 192, 195.195 and 205% respectively.,

Example 9, Example 8 is repeated. With the difference that the means of Example 1 is used, in the preparation of which calcium and potassium ions are used in the amount of 27 and 36 mmol

respectively (experiment A) or 33 and A4 mmol respectively (experiment B), and the following results were obtained; Experience A - 105%; Experience B - 160%.

Claims (1)

Comparison of the results of example 8 and comparative experiments A and B of example 9 show that when using calcium and potassium ions in a quantity less than the proposed lower limits, the goal is practically not achieved, and when the amount is greater than the upper limits, the positive effect begins to deteriorate. Invention Formula The method of preparing an agent for healing wounds by mixing heated water with a high molecular weight substance, adding an alkaline salt followed by cooling differs from and in order to improve wound healing due to the homogeneous distribution of the active substance to the potassium and calcium salts. the amount of 40 mmol of potassium ions and 30 mmol of calcium ions are added preservative-aikilparaben or its mixture with sorbitol, hydroxyl-containing alkylcellulose is dispersed as an amount of high molecular weight substance, providing viscosity of the agent 30- 90 Pa-s, cooling is carried out with stirring.