SU1409942A1 - Method of diagnostics of psoriasis - Google Patents

Abstract

The invention relates to dermatology, intended for the diagnosis of psoriasis, its stages, prediction, course and evaluation of the effectiveness of the therapy. The purpose of the invention is to improve the accuracy of diagnosis. For this and the patient's serum, add it to the culture of diploid human skin fibroplasts, assess the cytostimulating effect of serum on the inclusion of n-thymidine, calculate the stimulation index. When the index value is 30-105, an indication of the presence of a progressive stage of psoriasis is made, with an index of 2-11, a stationary or regressive stage of psoriasis is diagnosed. i (L

Description

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The invention relates to dermatology and can be used for the diagnosis of psoriasis, its stages, prediction, course and assessment of the effectiveness of therapy. I The purpose of the invention is to improve the accuracy of diagnosis by determining the stage of the disease.

Example 1. Patient R., / In the years, was admitted with complaints of exacerbation: itching, itching. Diagnosis: spread iibtft phoresis.

A patient from the ulnar vein is taken. ml of blood, by centrifuging I, 1 ml of serum was detected. The study was conducted on diploid fibroblasx of human skin. For cultivation, a nutrient medium is used with a set of amino acids, vitamins based on a balanced salt solution, necessary for cell growth, with

0% of cattle serum, 5% of human umbilical cord serum, cultivation of iodine t at 37 ° C in an atmosphere of 95% air and 5% CO.

A suspension of cells with a density of -10 cells per 1 ml of medium is introduced into 25 well plastic panels of 1O 2 ml per well. At the bottom of each moon there are 16-11 mm crocodile glasses of the same size. It is cultivated for 24 hours, then the medium is changed to Sessorothoid, cultivated for 48 hours, and the medium is changed for nutrient, containing 10% of the patient's serum. 10 hours after the medium change, midin is introduced at the rate of 1 µCi / ml and incubated for another 20 hours before fixation. Before fixation, cover glasses rtm the nutrient medium, fix cooled with a mixture of etarol and picosic acid (1: 3) 15 mi (the solvent solution components are washed with cold (4 ° C) 15 s for two minutes, then discarded E6% alcohol. Dried in the air are placed in vials of C with toluene syncitor and counted for reactivity using an A / C counter. Inclusion of H-thymidine in non-soluble in trichloacetic acid In Gmin on glass, serves DNA synthesis Telem.

The ratio of the values obtained 1 on the medium with the patient's serum, to

the value of a zero-short control is taken as the index from the feed. The latter is studied for the purpose of working out

diagnostic criteria in 56 patients with psoriasis and 12 healthy donors. As a result of the research, it was established that the progressive stage corresponds to an index value from 30 to 105,

from the stationary regressive stage - from 12 to 27, to healthy donors - to 12. In this example, the inclusion of midin - 29384 pulses / min; recurrent control - 320 pulses / min; stimulation index - 90.

Thus, the stimulation index is 90 units. shows that in a given patient the serum has a high cytostimulating effect, which

characteristic of the progressive stage of the disease. Specified diagnosis: pustular psoriasis. Progressive stage.

Example 2. Patient E.,

46 years old, number of case history 2606, was admitted to the clinic in April 1983, complaining of a rash, itching. Diagnosis: common exudative psoriasis. The patient underwent the study indicated in Example 1. The inclusion of H-thymidine is 32,433 cpm; serum-free control - i 320 imp / min; stimulation index - 105. The diagnosis is specified: a common

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exudative psoriasis. For patient 40

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one and a half months treatment has been administered. A repeat study was carried out to monitor the effectiveness of the treatment according to the procedure specified in Example 1. The result: the inclusion of H-thymidine - 2967 cpm, serum-free control - 320 cpm, the stimulation index - 9.2, which objectively records the effectiveness of the methods treatment.

Thus, the stimulation index for sera obtained from patients in the progressive stage of psoriasis is very high (higher than that of the strongest cell multiplication stimulator, human umbilical cord serum, whose stimulation index is 35-44 units). It should be noted that for more severe clinical forms of psoriasis (exudative, pustular, psoriatic erythroderma and drr), a higher stimulation index is characteristic.

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These persistent index differences aim to improve diagnostic accuracy.

sa stimulation depending on post-staging by determining the stages of the disease,

days of psoriasis increase the accuracy of the diag-serum is added to

nostica; which, in my own mind, is due to the diploid culture of diploid fibroplasts

It is correct to carry out the treatment and human skin, followed by assessment

to monitor its effectiveness. The cyto-stimulating effect of serum on the inclusion of H-thymidine with the calculation formula of the invention for the stimulation index and when its value is 30-105 is diagnosed by the method of diagnosing psoriasis through a progressive stage.

studies of the blood flow of patients, 2-11 - stationary or regressive, distinguished by the fact that it is similar to the stage of psoriasis.

Claims (1)

Claim A method for the diagnosis of psoriasis by examining the blood serum of patients, characterized in that, in order to improve the accuracy of diagnosis by determining the stages of the disease, the test serum is added to the culture of diploid fibroplasts of human skin, followed by assessment of the cytostimulating effect of serum on the inclusion of H ³ -thymidine with the calculation of the stimulation index and with a value of 30-105, a progressive stage is diagnosed, and with an index of 2-11, a stationary or regressing stage of psoriasis is diagnosed.