SU1318867A1 - Method of determining 1-hydrazinophthalazine hydrochloride - Google Patents
Method of determining 1-hydrazinophthalazine hydrochloride Download PDFInfo
- Publication number
- SU1318867A1 SU1318867A1 SU853948693A SU3948693A SU1318867A1 SU 1318867 A1 SU1318867 A1 SU 1318867A1 SU 853948693 A SU853948693 A SU 853948693A SU 3948693 A SU3948693 A SU 3948693A SU 1318867 A1 SU1318867 A1 SU 1318867A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- solution
- sensitivity
- color reagent
- simplification
- selectivity
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 13
- ZUXNZUWOTSUBMN-UHFFFAOYSA-N hydralazine hydrochloride Chemical compound Cl.C1=CC=C2C(NN)=NN=CC2=C1 ZUXNZUWOTSUBMN-UHFFFAOYSA-N 0.000 title claims abstract description 6
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims abstract description 12
- 230000035945 sensitivity Effects 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract 4
- 238000000605 extraction Methods 0.000 claims abstract 2
- 238000005375 photometry Methods 0.000 claims abstract 2
- FRASJONUBLZVQX-UHFFFAOYSA-N 1,4-naphthoquinone Chemical compound C1=CC=C2C(=O)C=CC(=O)C2=C1 FRASJONUBLZVQX-UHFFFAOYSA-N 0.000 claims description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims 2
- 238000007865 diluting Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- LOUPRKONTZGTKE-WZBLMQSHSA-N Quinine Chemical compound C([C@H]([C@H](C1)C=C)C2)C[N@@]1[C@@H]2[C@H](O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-WZBLMQSHSA-N 0.000 abstract description 4
- XQYZDYMELSJDRZ-UHFFFAOYSA-N papaverine Chemical compound C1=C(OC)C(OC)=CC=C1CC1=NC=CC2=CC(OC)=C(OC)C=C12 XQYZDYMELSJDRZ-UHFFFAOYSA-N 0.000 abstract description 4
- 229930008281 A03AD01 - Papaverine Natural products 0.000 abstract description 2
- 235000001258 Cinchona calisaya Nutrition 0.000 abstract description 2
- OGDVEMNWJVYAJL-LEPYJNQMSA-N Ethyl morphine Chemical compound C([C@H]1[C@H](N(CC[C@@]112)C)C3)=C[C@H](O)[C@@H]1OC1=C2C3=CC=C1OCC OGDVEMNWJVYAJL-LEPYJNQMSA-N 0.000 abstract description 2
- OGDVEMNWJVYAJL-UHFFFAOYSA-N Ethylmorphine Natural products C1C(N(CCC234)C)C2C=CC(O)C3OC2=C4C1=CC=C2OCC OGDVEMNWJVYAJL-UHFFFAOYSA-N 0.000 abstract description 2
- RPTUSVTUFVMDQK-UHFFFAOYSA-N Hidralazin Chemical compound C1=CC=C2C(NN)=NN=CC2=C1 RPTUSVTUFVMDQK-UHFFFAOYSA-N 0.000 abstract description 2
- XOZFCTKEUWXPAF-UHFFFAOYSA-N apressin Natural products CC1(O)C(OC(=O)C)CC2C(=C)C(=O)OC2C2C3(C)C=CC21OO3 XOZFCTKEUWXPAF-UHFFFAOYSA-N 0.000 abstract description 2
- 150000007514 bases Chemical class 0.000 abstract description 2
- LOUPRKONTZGTKE-UHFFFAOYSA-N cinchonine Natural products C1C(C(C2)C=C)CCN2C1C(O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-UHFFFAOYSA-N 0.000 abstract description 2
- 229960004578 ethylmorphine Drugs 0.000 abstract description 2
- 229960001789 papaverine Drugs 0.000 abstract description 2
- REQCZEXYDRLIBE-UHFFFAOYSA-N procainamide Chemical compound CCN(CC)CCNC(=O)C1=CC=C(N)C=C1 REQCZEXYDRLIBE-UHFFFAOYSA-N 0.000 abstract description 2
- 229960000244 procainamide Drugs 0.000 abstract description 2
- 229960000948 quinine Drugs 0.000 abstract description 2
- 230000002921 anti-spasmodic effect Effects 0.000 abstract 1
- 239000000812 cholinergic antagonist Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- JGOAIQNSOGZNBX-UHFFFAOYSA-N 2,2-diphenylacetic acid 2-(diethylamino)ethyl ester Chemical compound C=1C=CC=CC=1C(C(=O)OCCN(CC)CC)C1=CC=CC=C1 JGOAIQNSOGZNBX-UHFFFAOYSA-N 0.000 description 1
- PIJVFDBKTWXHHD-UHFFFAOYSA-N Physostigmine Natural products C12=CC(OC(=O)NC)=CC=C2N(C)C2C1(C)CCN2C PIJVFDBKTWXHHD-UHFFFAOYSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000002220 antihypertensive agent Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- PIJVFDBKTWXHHD-HIFRSBDPSA-N physostigmine Chemical compound C12=CC(OC(=O)NC)=CC=C2N(C)[C@@H]2[C@@]1(C)CCN2C PIJVFDBKTWXHHD-HIFRSBDPSA-N 0.000 description 1
- 229960001697 physostigmine Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
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- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Изобретение относитс к аналитической химии, в частности к определению 1-гидразинофталазина гидрохлорида (апрессина). Повышение чувствительности , селективности и упрощение способа достигаютс использованием другого цветореагента и среды. Определение ведут растворением анализируемой пробы в воде, обработкой полученного раствора цветореагентом Ы-толуолсульфонил-2-хлор-1,4-нафто- хинонимином в среде диметилформамида с последующим фотометрированием раствора . Чувствительность способа 8- 28 мкг/мл. Селективность способа обеспечиваетс тем, что N-толуолсуль- фонил-2-хлор-1,4-нафтохинонимин не взаимодействует с хинином, новокаин- амидом, папаверином, физостиглином, спазмолитиком, этилморфином и другими соединени ми основного характера . Упрощение способа достигаетс исключением стадии экстрагировани . 2 табл. ( (/) СдО 00 00 о This invention relates to analytical chemistry, in particular to the determination of 1-hydrazinophthalazine hydrochloride (apressin). Increasing the sensitivity, selectivity and simplification of the method is achieved by using a different color reagent and medium. The determination is carried out by dissolving the analyzed sample in water, by treating the resulting solution with the color reagent N-toluenesulfonyl-2-chloro-1,4-naphthoquinonimine in dimethylformamide medium, followed by photometry of the solution. The sensitivity of the method is 8-28 μg / ml. The selectivity of the method is ensured by the fact that N-toluenesulfonyl-2-chloro-1,4-naphthoquinone imine does not interact with quinine, novocaine amide, papaverine, physostigline, antispasmodic, ethyl morphine and other basic compounds. The simplification of the process is achieved with the exception of the extraction stage. 2 tab. ((/) SdO 00 00 o
Description
113113
Изобретение относитс к аналитической химии, а именно к способам количественного определени 1-гидрази- нофталазина гидрохлорида (апрессина) примен емого в медицинской практике как гипотензивное средство.The invention relates to analytical chemistry, and specifically to methods for the quantitative determination of 1-hydrazinophthalazine hydrochloride (apressin) used as a hypotensive agent in medical practice.
Целью изобретени вл етс повышение чувствительности, селективности и упрощение способа.The aim of the invention is to increase the sensitivity, selectivity and simplification of the method.
Способ осуществл етс следующим образом.The method is carried out as follows.
Пример. Количественное определение 1-гидразинофталазина гидрохлорида в субстанции. Точную навеску анализируемого вещества в пределах 0,010-0,020 г раствор ют в воде в мерной колбе вместимостью 50 мл и до вод т до метки этим же растворителем 1 мл полученного раствора помещают в мерную колбу вместимостью 25 мл, прибавл ют 3 мл 0,5%-ного раствора N-TO луолсульфонил-2-хлор-1,4-нафтохинон- имина в диметилформамиде и довод т диметилформамидом до метки. Параллел но провод т опыт со стандартным раст вором 1-гидразинофт-алазина гидрохлорида и контролем.Example. Quantitative determination of 1-hydrazinophthalazine hydrochloride in the substance. The exact weight of the analyte in the range of 0.010-0.020 g is dissolved in water in a 50 ml volumetric flask and to water up to the mark with the same solvent, 1 ml of the resulting solution is placed in a 25 ml volumetric flask, 3 ml of 0.5% is added. Ny-luosulfonyl-2-chloro-1,4-naphthoquinone-imine solution in dimethylformamide and bring it to the mark with dimethylformamide. A parallel experiment was carried out with a standard solution of 1-hydrazinoft-alazine hydrochloride and control.
Оптическую плотность анализируемы растворов измер ют на фоне контрол при помощи спектрофотометра при 537 нм в кюветах-с толщиной сло 1 сThe optical density of the analyzed solutions is measured against the control using a spectrophotometer at 537 nm in cuvettes with a layer thickness of 1 s
Расчет количественного содержани 1-гидразинофтапазина гидрохлорида (%) провод т по формулеThe calculation of the quantitative content of 1-hydrazinophapazine hydrochloride (%) is carried out according to the formula
Р.1250-Со D,.p . Е R.1250-Co D, .p. E
где D - оптическа плотность анализируемого раствора;where D is the optical density of the solution being analyzed;
j j
JO JO
t5 20 t5 20
2525
DQ - оптическа плотность стандартного раствора; . С - концентраци стандартного спектрофотометрируемого раствора (0,0012 г в 100 мл); Р - навеска, г; t - толщина сло , см. Результаты количественного определени 1-гидразинофталазина гидрохЛо- рида в субстанции приведены в ,табл.1. Сравнительна характеристика предлагаемого и известного способов приведена в табл.2.DQ is the optical density of the standard solution; . C is the concentration of a standard spectrophotometric solution (0.0012 g in 100 ml); P — weight, g; t is the layer thickness, see. The results of the quantitative determination of 1-hydrazinophthalazine hydrochloride in the substance are given in Table 1. Comparative characteristics of the proposed and known methods are given in table 2.
Как видно из табл.2, предлагаемый способ по величине чувствительности превосходит известный в 10-21 раз и вл етс более простым. Кроме того, способ вл етс более селективным, так как Ы-толуолсульфонил-2-хлор- . 1,4-нафто-хинонимин не взаимодействует с хинином, новокайнамидом, папаверином , физостигмином, спазмолити- ном, этилморфином и другими соединени ми основного характера.As can be seen from Table 2, the proposed method exceeds the known sensitivity by a factor of 10-21 and is simpler. In addition, the process is more selective, since L-toluenesulfonyl-2-chloro. 1,4-naphtho-quinone imine does not interact with quinine, Novocainamide, papaverine, physostigmine, spasmolytin, ethyl morphine and other basic compounds.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU853948693A SU1318867A1 (en) | 1985-07-02 | 1985-07-02 | Method of determining 1-hydrazinophthalazine hydrochloride |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU853948693A SU1318867A1 (en) | 1985-07-02 | 1985-07-02 | Method of determining 1-hydrazinophthalazine hydrochloride |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1318867A1 true SU1318867A1 (en) | 1987-06-23 |
Family
ID=21195721
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU853948693A SU1318867A1 (en) | 1985-07-02 | 1985-07-02 | Method of determining 1-hydrazinophthalazine hydrochloride |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU1318867A1 (en) |
-
1985
- 1985-07-02 SU SU853948693A patent/SU1318867A1/en active
Non-Patent Citations (1)
| Title |
|---|
| Danietson N.D., BartoЕо R.G. Determination of hydratarine in tab- Eetes by fEuorescence. - Anal . ZetP, 1983, vol. 16, № 15, p. 343. Государственна фармакопе СССР X издани . М.: Медицина, 1968, с. 104. Коренман И.М. Фотометрический анализ. Методы определени органических соединений. М.: Хими , 1975, с. 223. * |
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