SU1289500A1 - Method of microbiological decontamination of metal surface - Google Patents

Abstract

The invention relates to methods of microbiological decontamination of metal products and can be used in the treatment of surfaces of devices, equipment and technological premises. The purpose of the invention is to increase the reliability of decontamination treatment and reduce the corrosivity of oxidizers used, using perchlorates as oxidizers and surface treatment at an oxidizer consumption rate of 0.3–0.5 l / m, concentration on active chlorine 1.5–3 , 5% and exposure 3-6 hours. 6 table. (Lgo 00 o JV

Description

1i

The invention relates to methods I of microbiological decontamination of metal products and can be used in the treatment of surfaces of devices, equipment and technological premises.

The purpose of the invention is to increase the reliability of the decontamination treatment and reduce the corrosion activity of the used oxidizing agents.

The essence of the method is that when treating metal surfaces with oxidizing agents containing active chlorine, perchlorate solutions are used as oxidizing agents, and the treatment is carried out at an oxidizer consumption rate of 0.3 to 0.5 l / m, the concentration of active chlorine from 1.5 to 3.5% and exposure time from 3 to 6 h.

The embodiment of the method is illustrated by examples. In the quality of oxidizing agents, carbamide perchlorate, thiocarbamide perchlorate and divalent copper perchlorate were used.

An indicator of the oporocidal action is the value of the kinetics constant of decontamination of a stable form of spore microorganisms B. anthracoTdes, pcs. 250, titer of n-Yu dispute / m

The value of the kinetics constants of inactivation of spores in. anthracoides, pieces 250, titer n; 10 spores / ml of perchlorate and chloramine solutions, taken as a comparison decontaminate, are listed in Table. one,

To determine the constant, a spore suspension was taken from B. anthracoidee, pcs. 250, the titer of p. -10 spores / MP and mixed with a solution of a decontaminant in a 1: 1 ratio.

The solution of chloramine was taken in concentrations used in practice. At certain intervals, depending on the concentration of the decontaminant at 5% (1.69% active chlorine) after 60 min, at 10% {2.65% active chlorine) after 30 min, at 15% (5.69% active chlorine) after 10–15 min, a sample was taken (1 ml) in the same amount of sodium thiosulfate neutralizer of the corresponding concentration. Neutralized sample was rationed. Sowing was performed at 1 ml per solid nutrient medium. The results were taken into account after daily incubation at 28-37 ° C. The numbers of surviving microorganisms were counted.

From the data table. 1 it follows that all recommended formulations have a sporicidal effect, which proves the reliability of decontamination, and compared to 5 used chloramines, they do not have a sharp, specific smell of chlorine caused by irritation of the mucous membranes. 10 Examples of alloys of basic structural materials (alloyed and unalloyed steels, aluminum AMC) were treated with solutions of decontaminating recipes with the same content of active substance.

In tab. 2 shows data on the corrosivity of known formulations and those proposed on the basis of perchlorates.

20 The data given in table. 2, testify to the resistance of the tested materials to the proposed recipes, and alloyed and non-alloyed steels are much more resistant to the proposed recipes than to chloramine, taken in a lower concentration, with the exception of aluminum surfaces that are unstable with respect to chlorine-containing compounds.

Test surfaces and tools were contaminated with spore suspension. anthracoides with a concentration of 10 spores / ml according to Instruction J5 for the determination of the disinfecting activity of new bactericidal substances, VNIIDiS, 1968. The contamination control is common to all three treatment modes.

40 Decontamination was carried out with perchlorate solutions by irrigation with an air flow rate of 10 l / min, decontamination solution flow rate of 10 ml / min and irrigation density of 45 0.5 l / m, exposure for 6 hours at a chlorine concentration of 3.5%, respectively. After appropriate exposure, flushing and seeding was performed according to the specified instructions. The results were recorded after daily incubation at.

The optimal parameters of decontreatment treatment of the surfaces of objects (concentration on chlorine 2.5% 55 exposure 4 h, the consumption rate 0.4 l Ai are given in Table 3.

When deviating from the recommended method parameters, the effect of surface decontamination is not observed.

The parameters of the decontamination treatment of the surfaces of the objects (chlorine concentration 1, 2 hours are given in Table 4.

exposure

/

The consumption rate of 0.2 l / m). According to these tables, the conclusion is drawn about the reliability of the proposed tool processing modes, and the metal test surfaces used for manufacturing equipment and instruments, all of these modes result in complete decontamination of objects, since the processing time is specifically recommended more calculated in. avoidance of single growth of especially resistant populations of microorganisms.

The proposed decontamination agents have a sporicidal effect, which leads to a reliable decontaminating effect of objects contaminated by vegetative and

Test time About 12 3

The content of perchlorate ion,% 6,26

6.286.28 6.29 6.316.31 6.32 6.32 6.32 6.32

The sporicidal activity of the neutral - including their treatment with the help of the called and working solution of perchlo-JQ oxidants, containing active

 The carbamide resin is given in Table. 5. The neutralization verification data are given for carbamide perchlorate with a chlorine content of 2.5%. Neutralization was performed with 10% sodium thiosulfate solution in a 1: 1 ratio.

Claims (1)

Invention Formula The method of microbiological decontamination of metal surfaces. 35 40 chlorine, characterized in that, in order to increase the reliability of the decontamination treatment and decrease the corrosion activity of the oxidizers, perchlorate solutions are used as oxidizers, and the treatment is carried out at a consumption rate of 0.3 to 0.5 l / m% concentration The total chlorine content is from 1.5 to 3.5% and the expo positions are from 3 to 6 h. j Table 1 Carbamide perchlorate I Thiocarbamide perchlorate Bivalent copper perchlorate spore form of microorganisms. The main structural materials are resistant to the proposed decontaminating agents (perchlorates) in a greater degree than to a known decontaminating agent, since the value of the depth indicator of the corrosion rate is less. Perchlorates emit chemical industrial. Aqueous solutions of perchlorates are stable during long-term storage and are reliably neutralized with sodium thiosulfate solution. Stability of water solutions carbamide perchlorate, total concentration 10%, perchlorate-yo content, by 6.2%. 20 The solution was stored in a glass dish in the light under normal conditions. Gas release was not observed. . 7 eleven 13 15 18 35 40 chlorine, characterized in that, in order to increase the reliability of the decontamination treatment and reduce the corrosion activity of oxidizing agents, perchlorate solutions are used as oxidizing agents, and the treatment is carried out at a consumption rate of 0.3 to 0.5 l / m% concentration of active chlorine from 1 5 to 3.5% and exposure from 3 to 6 h. J Table 1 Note. + solid growth. - lack of growth. Table 2 Table 3 Scalpels Control + + + 200, 242 18, 21 2; five + + + + + Note. + - solid growth - - lack of growth.  I. Table 5 Type and titer of Decontamcian-Neutralized - Control of the culture of the hand, prescription solution of culture decontamination recipes Strain 250 10 SP / MT - ++ Note, - continuous growth. - - lack of growth. Compiled by E. Ilyin, Editor A. Dolinich Tehred V. Kadar Corrector B, But ha Order 7837/6 Circulation 617 Subscription VNIIGSh of the USSR State Committee for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5 Production and printing company, Uzhgorod, st. Project, 4 Table 4 Metal test surfaces Control - ++ - ++ - + - ++ - f- + 238, ---- 218 19, ---- 24 2; four---