SU1262376A1 - Method of determining half-period of erythrocyte aggregation - Google Patents

Abstract

The invention relates to the field of medicine, namely physiology, and may find application in hematology. The purpose of the invention is to increase the sensitivity of the method. The investigated blood sample is drawn through the fitting with a syringe into a micro cuvette washed with heparin and dried. The fitting is closed with a microtube and is connected via an electrical connector to a standard power source of a piezoelectric element and a standard measuring device. round. The micro cuvette is installed horizontally, the light is fed vertically. From the microscope mirror through a typical condenser, a blood sample, the optical system of the microscope to the photocell. The amplitude and frequency of the mechanical oscillations of the piezoelectric element necessary for complete disaggregation are set by the output voltage and frequency of the power supply. The amplitude is 0.1–1.0 µm, (f. Frequency 1.0–20 kHz. Mechanical action perpendicular to the surface of the test sample.

Description

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u: The invention relates to medi1 (nei, namely physiology (hematology). The purpose of the invention is to increase the sensitivity of the method due to the fact that the disaggregation is carried out by the piez element, operating in the audio frequency range. The method is carried out as follows. The test (capill plenary, venous, arterial) blood is drawn by a syringe through a squirt into a special cuvette, previously washed with heparin (prepared at the rate of 500 units per 10 ml of blood) and dried. The plugs are connected to a standard piezo cell power source and standard measuring equipment. The micro cuvette is mounted horizontally on a biological microscope stage. and the frequency of the power supply set the amplitude and frequency of the mechanical oscillations of the piezoelectric element and the rigidly connected wall of the cell. imye for full disaggregation ,. The power source is applied and the destruction is performed by mechanical oscillations with an amplitude of 0.1-1.0 µm and a frequency of 1.0–20.0 kHz, while the mechanical power is directed perpendicular to the surface of the test sample. Changes in the optical density of the blood sample under study, which are associated with reversible aggregation of red blood cells, are converted into an electrical signal by a photocell, amplified by an amplifier, and recorded onto a paper tape by a self-detector. The degree of disaggregation is monitored during mixing by photometric and microscopic examination. After reaching complete disaggregation, which corresponds to the maximum optical density of the investigated strain, turn off the power supply of the piezoelectric element and (by the change in optical density) record the process of spontaneous aggregation of red blood cells. The erythrocyte aggregation half-life is calculated based on the measurement results of the photometric curve recorded

Claims (1)

destruction of the erythrocyte aggregates with subsequent determination of the optical density of the blood sample being examined, by an am-recorder, the duration of aggregation of the rem, during which the amplitude of the isometric signal changes and 50% the yield of the photometric curve on the plateau corresponding to the complete aggregation of red blood cells - the optical density of the blood sample under investigation is minimal). The method allows to increase the accuracy of the study, since the magnitude of the half-cycle of erythrocyte aggregation, which characterizes the kinetics of erythrocyte aggregation, depends on the degree of their disaggregation. An example. A mechanical effect on the blood samples of a human, dog, scab, rat, mouse is performed with an amplitude of the order of 0.1 µm and, respectively, with frequencies of 1.0 and 20.0 kHz. The incomplete disaggregation of erythrocytes is indicated by the small amplitude of the photometric signal and the Hsshichie characteristic peak on the fluorimetric curve at the moment of stopping the mixing. Next, mechanical action is carried out at optimal values of the frequency and amplitude of oscillations for mouse erythrocytes (ttg 13.0 kHz, Ag 0.5 µm). The complete disaggregation of erythrocytes is evidenced by the absence of a characteristic peak at the time of stopping the mixing, as well as a higher amplitude of the signal of the same blood sample. The numerical values of the half-period of α-aggregation, determined on the basis of the calculation of photometric curves, also differ. 40.0; 105.0 and 70., 0 s, respectively. The application of the proposed method, based on the analysis of a lower blood dose (0.005 ml instead of 0.01 ml according to a known method), allows to increase the sensitivity of measurements by 4 - 5 times. The invention The method for determining the half-period, aggregation of red blood cells by mechanical, 12623764 .. characterized in that c1.0 tiKM and a frequency of 1.0-20.0 kHz, In order to increase the sensitivity of the mechanical impact of the soba, the destruction is carried out mechanically-directed perpendicular to the surface oscillations with amplitude O, 1-of the sample under study.