SU1127545A1 - Nutrient medium for growing hyacinth in vitro - Google Patents
Nutrient medium for growing hyacinth in vitro Download PDFInfo
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- SU1127545A1 SU1127545A1 SU823402661A SU3402661A SU1127545A1 SU 1127545 A1 SU1127545 A1 SU 1127545A1 SU 823402661 A SU823402661 A SU 823402661A SU 3402661 A SU3402661 A SU 3402661A SU 1127545 A1 SU1127545 A1 SU 1127545A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- sodium
- potassium
- sulfate
- phosphate
- thiamine
- Prior art date
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- 235000015097 nutrients Nutrition 0.000 title claims description 6
- 238000000338 in vitro Methods 0.000 title claims description 3
- 241001632576 Hyacinthus Species 0.000 title claims 2
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims abstract description 12
- 239000002253 acid Substances 0.000 claims abstract description 8
- 229930006000 Sucrose Natural products 0.000 claims abstract description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000011702 manganese sulphate Substances 0.000 claims abstract description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims abstract description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 6
- 239000005720 sucrose Substances 0.000 claims abstract description 6
- 229960003495 thiamine Drugs 0.000 claims abstract description 6
- 239000011721 thiamine Substances 0.000 claims abstract description 6
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000019157 thiamine Nutrition 0.000 claims abstract description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims abstract description 6
- 229920001817 Agar Polymers 0.000 claims abstract description 5
- 239000008272 agar Substances 0.000 claims abstract description 5
- 229910000365 copper sulfate Inorganic materials 0.000 claims abstract description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 5
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 claims abstract description 5
- 239000005972 6-Benzyladenine Substances 0.000 claims abstract description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 4
- -1 Cobalt chloride Sodium molybdenum Chemical compound 0.000 claims abstract description 4
- 239000001110 calcium chloride Substances 0.000 claims abstract description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 4
- 229960000367 inositol Drugs 0.000 claims abstract description 4
- 229940099596 manganese sulfate Drugs 0.000 claims abstract description 4
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims abstract description 3
- 229960004839 potassium iodide Drugs 0.000 claims abstract description 3
- 229910000160 potassium phosphate Inorganic materials 0.000 claims abstract description 3
- 235000011009 potassium phosphates Nutrition 0.000 claims abstract description 3
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims abstract description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 3
- 102000002397 Kinins Human genes 0.000 claims abstract 3
- 108010093008 Kinins Proteins 0.000 claims abstract 3
- 229910000358 iron sulfate Inorganic materials 0.000 claims abstract 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract 2
- 229960001763 zinc sulfate Drugs 0.000 claims abstract 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
- 239000011591 potassium Substances 0.000 claims description 5
- 229910052700 potassium Inorganic materials 0.000 claims description 5
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 3
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 2
- 239000004327 boric acid Substances 0.000 claims description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims 2
- 229910021653 sulphate ion Inorganic materials 0.000 claims 2
- YJGNZVVNOURNJV-UHFFFAOYSA-K [Mg+2].[K+].OP(O)([O-])=O.[O-]S([O-])(=O)=O Chemical compound [Mg+2].[K+].OP(O)([O-])=O.[O-]S([O-])(=O)=O YJGNZVVNOURNJV-UHFFFAOYSA-K 0.000 claims 1
- FPTJTJNTUMJHNK-UHFFFAOYSA-N [Na].CC(=O)ON.CC(=O)ON.CC(=O)OCCOC(C)=O Chemical compound [Na].CC(=O)ON.CC(=O)ON.CC(=O)OCCOC(C)=O FPTJTJNTUMJHNK-UHFFFAOYSA-N 0.000 claims 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- MCXBMLBTPQEQJP-UHFFFAOYSA-N potassium;sodium;dinitrate Chemical compound [Na+].[K+].[O-][N+]([O-])=O.[O-][N+]([O-])=O MCXBMLBTPQEQJP-UHFFFAOYSA-N 0.000 claims 1
- ZVFGWPAZJXRXNB-UHFFFAOYSA-L sodium molybdenum(4+) sulfate Chemical compound [Mo+4].S(=O)(=O)([O-])[O-].[Na+] ZVFGWPAZJXRXNB-UHFFFAOYSA-L 0.000 claims 1
- 239000011734 sodium Substances 0.000 abstract description 4
- 229910052708 sodium Inorganic materials 0.000 abstract description 4
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 abstract description 4
- 239000001488 sodium phosphate Substances 0.000 abstract description 3
- 229910000162 sodium phosphate Inorganic materials 0.000 abstract description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract description 2
- 239000011684 sodium molybdate Substances 0.000 abstract description 2
- 235000015393 sodium molybdate Nutrition 0.000 abstract description 2
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 abstract 1
- DCPVGCYTIBNGRQ-UHFFFAOYSA-L [Mg+2].[K+].[O-][N+]([O-])=O.[O-]S([O-])(=O)=O Chemical compound [Mg+2].[K+].[O-][N+]([O-])=O.[O-]S([O-])(=O)=O DCPVGCYTIBNGRQ-UHFFFAOYSA-L 0.000 abstract 1
- DVARTQFDIMZBAA-UHFFFAOYSA-O ammonium nitrate Chemical class [NH4+].[O-][N+]([O-])=O DVARTQFDIMZBAA-UHFFFAOYSA-O 0.000 abstract 1
- 235000007715 potassium iodide Nutrition 0.000 abstract 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 239000010941 cobalt Substances 0.000 description 5
- 229910017052 cobalt Inorganic materials 0.000 description 5
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
- 229910052742 iron Inorganic materials 0.000 description 3
- 239000011777 magnesium Substances 0.000 description 3
- 229910052749 magnesium Inorganic materials 0.000 description 3
- 229960004793 sucrose Drugs 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 2
- 235000011008 sodium phosphates Nutrition 0.000 description 2
- 239000011701 zinc Substances 0.000 description 2
- 229910052725 zinc Inorganic materials 0.000 description 2
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N Indole-3-acetic acid Natural products C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 150000008575 L-amino acids Chemical class 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 241001420622 Meris Species 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- PRORZGWHZXZQMV-UHFFFAOYSA-N azane;nitric acid Chemical compound N.O[N+]([O-])=O PRORZGWHZXZQMV-UHFFFAOYSA-N 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011708 vitamin B3 Substances 0.000 description 1
- 235000019160 vitamin B3 Nutrition 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
Classifications
-
- Y02P60/216—
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
ПИТАТЕЛЬНАЯ СРЕДА ДЛЯ ВЫРАЩИВАНИЯ ГИАЦИНТОВ IN HTRO, содержаща натрий фосфорнокислый двузамещенный , аммонТ1Й азотнокислый, кальций хлористый, этилендиамйнотетраацетат натри , калий азотнокислый, магний сернокислый, калий фосфорнокислый однозамещенный , железо сернокислое, цинк сернокислый , борную кислоту, марганец сернокислый , медь сернокислую, кобальт хлористый , натрий молибденовокислый, калий йодистый , тиамин, мезоинозит, кинин, сахарозу , агар и воду, отличающа с тем, что, с целью увеличени выхода луковиц, среда дополнительно содержит дестиобиотин, а в качестве кинина- 6-бензиладенин при следующем соотношении компонентов, мг/л: Натрий фосфорнокислый 169,0-172,0 двузамещенный Аммоний азотнокислый 1640,0-1660,0 Кальций хлористый 435,0-445,0 Этилендиамннотетрааце37 ,2-37,4 тат натри 1850,0-1910,0 Калий азотнокислый Магний сернокислый 365,0-375,0 Калий фосфорнокислый 169,0-171,0 однозамещенный 27,5-28,0 Железо сернокислое 8,5-8,7 Цинк сернокислый 6,0-6,4 Борна кислота 22-22,5 Марганец сернокислый 0,024-0,025 с Медь сернокисла 0,024-0,025 Кобальт хлористый Натрий молибденово (Л 0,24-0,25 кислый 0,8-0,85 Калий йодистый 0,9-1,1 Тиамин 1.00,0-150,0 Мезоинозит 0,1-2,0 6-Бензнладенин 0,01-0,1 Дестиобиотин Г 15000,0-20000,0 Сахароза 5000,07000 ,0 Агар До1.л Вода СП 4 слNUTRITING AMERICAN CASES sodium molybdate, potassium iodide, thiamine, meso-inositol, kinin, sucrose, agar and water, characterized in that, in order to increase the output of the bulbs, the medium additionally will contain destiobiotin, and as kinin- 6-benzyladenine in the following ratio of components, mg / l: Sodium phosphate 169.0-172.0 disubstituted Ammonium nitrate 1640.0-1660.0 Calcium chloride 435.0-445.0 Ethylene diamine tetraace37, 2-37.4 tat sodium 1850.0-1910.0 Potassium nitrate Magnesium sulfate 365.0-375.0 Potassium phosphate 169.0-171.0 monosubstituted 27.5-28.0 Iron sulfate 8.5-8, 7 Zinc sulfate 6.0-6.4 Borna acid 22-22.5 Manganese sulfate 0.024-0.025 with Copper sulfate 0.024-0.025 Cobalt chloride Sodium molybdenum (L 0.24-0.25 acid 0,8-0,85 Potassium iodide 0.9-1.1 Thiamine 1.00.0-150.0 Mezo-inosite 0.1-2.0 6-Benznladenin 0.01-0.1 Destobiotin G 15000.0-20000.0 Sucrose 5000.07000, 0 Agar Do1.l Water SP 4 cl
Description
Изобретение относитс к получению безвирусного посадочного материала методом выращивани меристем и тканевых культур на искусственных питательных средах. Известна питательна среда дл выращивани растений из меристем, содержаща L-аминокислоты и витамины, в том числе биотин 1. Однако данна среда предназначена дл тканевых культур л действие биотина как активатора корнеобразовани нейтрализуетс цитокининами. Известна также среда Мурасиге-Скуга, обеспечивающа нормальное развитие ме% ристем разных культур и содержаща макро и микроэлементы, биологически активные вещества при следующем соотношении компонентов , мг/л: Натрий фосфорнокислый двузамещенный170,0 Аммоний азотнокислый 1650,0 Кальций хлористый440,0 Калий азотнокислый1900,0 Магний сернокислый370,0 Калий фосфорнокислый однозамещенный170,0 Железо сернокислое27,8 Натрий ЭДТА37,3 Цинк сернокислый8,6 Борна кислота6,2 Марганец сернокислый22,3 Медь сернокисла .0,025 Кобальт хлористый0,025 Натрий молибденовокислый 0,25 Калий йодистый0,83 Тиамин0,4 Пиридоксин0,5. Витамин РР 0,5 Глицин.2,0 Аденин80,0 Гидролизат казеина.1.000,0 Мезоинозит100,0 Jb -Индолилуксусна кислота2,0 Кинетин.0.2 Гиббереллова кислота . 0,1 Сахароза.30-000,0 АгарГО.000,0 Вода До 1л Однако дл известной питательной ереды характерно сравнительно длительное развитие корневой системы меристемы, увеличивающее сроки роста саженца (около 27 недель), что уменьшает выход саженцев и снижает производительность установки по культивированию, увеличивает себестоимость продукции. Кроме того, многокомпонентность биологически активных сеодинений усложн ет приготовление среды и удорожает последнюю . Цель изобретени - увеличение выхода луковиц. Поставленна цель достигаетс тем, что ательна среда дл выращивани гиатов in vitro содержаща натрий фосфорислый двузамещенный, аммоний азотнолый , кальций хлористый, этилендиаминораацетат натри , калий азотнокислый, нитй сернокислый, калий фосфорнокисоднозамещенный , железо сернокислое, к сернокислый, борную кислоту, маргасернокислый , медь сернокислую, коьт хлористый, натрий молибденовокис , калий йодистый, тиамин, мезоинозит, ин, сахарозу, агар и воду, дополнительсодержит дестиобиотин, а в качестве киа - 6-бензиладенин при следующем тношении компонентов, мг/л: Натрий фосфорнокислый двузамещенньш.169,0-172,0 Аммоний азотнокислый 1.640,0-1660,0 Кальций хлористый435,0-445,0 Этилендиаминотетраацетат натри 37,2-37,4 Калий азотнокислый1850,0-1910,0 Магний сернокислый365,0-375,0 Калий фосфорнокислый однозамещенный169,0-171,0 Железо сернокислое27,5-28,0 Цинк сернокислый..8,,7 Борна кислота..6,0-6,4 Марганец сернокислый 22,0-22,5 Медь сернокисла .0,024-0,025 Кобальт хлористый.0,024-0,025 Натрий молибденовокислый0 ,24-0,25 Калий йодистый0,8-0,85 Тиамин .0,9-1,1 Мезоинозит .100,0-150,0 6-Бензиладенин .0,1-2,0 Дестиобиотин 0,01-0,1 Сахароза 15000,0-20000,0 Агар 5000,0-7000,0 Вода До 1 л Пример 1. Питательную среду готов т ем ра.створени в бидистиллированной е при следующем соотношении компотов , мг/л: Натрий фосфорнокислый двузамещенный169,0 Аммоний азотнокислый 1640,0 Кальций хлористый.435,0 Этилендиаминотетраацетат натри 37,2 Калий азотнокислый1850,0 Магний сернокислый365,0 Калий фосфорнокислый однозамещенный169,0 Железо сернокислое27,5 Цинк сернокислый8,5 Борна кислота6,0 Марганец сернокислый 22,0 Медь сернокисла 0,024 Кобальт хлористый.0,024 Натрий молибденовокислый 6,24The invention relates to the production of virus-free planting material by growing meristem and tissue cultures on artificial nutrient media. The known nutrient medium for growing plants from meristem, containing L-amino acids and vitamins, including biotin 1. However, this medium is intended for tissue cultures and the action of biotin as a root-activator is neutralized by cytokinins. The Murashige-Skooga medium is also known, which ensures the normal development of meris of various cultures and contains macro and microelements, biologically active substances in the following ratio of components, mg / l: Sodium phosphate disubstituted170.0 Ammonium nitric acid 1650.0 Calcium chloride440.0 Potassium nitric acid1900, 0 Magnesium sulphate370.0 Potassium monosubstituted phosphate170.0 Iron sulphate27.8 Sodium EDTA37.3 Zinc sulphate8.6 Boric acid6.2 Manganese sulphate22.3 Copper sulfate .0.025 Cobalt chloride0.025 Sodium molybdenum oxide ly 0.25 Potassium yodistyy0,83 Tiamin0,4 Piridoksin0,5. Vitamin PP 0.5 Glycine.2,0 Adenine 80,0 Casein hydrolyzate. 1.000,0 Mezo-inositol 100,0 Jb - Indoleacetic acid 2,0 Kinetin. 0.2 Gibberelova acid. 0.1 Saccharose. 30-000.0 AgarGO 000.0 Water Up to 1 l However, for a known nutrient medium, a relatively long development of the root system of the meristem is typical, which increases the growth time of the seedling (about 27 weeks), which reduces cultivation, increases the cost of production. In addition, the multicomponent nature of biologically active compounds complicates the preparation of the medium and increases the cost of the latter. The purpose of the invention is to increase the output of the bulbs. The goal is to ensure that the in vitro environment is used to grow the giats. Chloride, sodium molybdenum oxide, potassium iodide, thiamine, meso-inositol, in, sucrose, agar, and water, additionally contain destobiotin, and as kia, 6-benzyl-ydenine with in relation to components, mg / l: Sodium phosphate disubstituted.169.0-172.0 Ammonium nitrate 1.640.0-1660.0 Calcium chloride435.0-445.0 Ethylene diamine tetraacetate sodium 37.2-37.4 Potassium nitrate 1850.0-1910 , Magnesium sulphate365.0-3-3.0.0 Potassium phosphate monosubstituted169.0-171.0 Iron sulphate27.5-28.0 Zinc sulphate..8,, 7 Boric acid..6.0-6.4 Manganese sulphate 22, 0-22.5 Copper sulfate .0.024-0.025 Cobalt chloride.0.024-0.025 Sodium molybdate, 24-0.25 Potassium iodide0.8-0.85 Thiamine .0.9-1.1 Mezo-inosit .100.0-150, 0 6-Benzyladenine .0.1-2.0 Destobiotin 0.01-0.1 Sucrose 15000.0-20000.0 Aga 5000.0-7000.0 Water Up to 1 l Example 1. A nutrient medium is prepared by dissolving it in double-distilled e at the following ratio of compotes, mg / l: Sodium disodium phosphate 169.0 Ammonium nitrate 1640.0 Calcium chloride.435.0 Ethylene diamine tetraacetate sodium 37.2 Potassium nitrate 1850.0 Magnesium sulphate365.0 Potassium monosubstituted phosphate 169.0 Iron sulphate27.5 Zinc sulphate8.5 Borna acid6.0 Manganese sulphate 22.0 Copper sulfate 0.024 Cobalt acid Cobalt acid 5.0.0 Manganese sulfate 0.024 Cobalt acid 2.0.0 Cobalt acid 3.0.0 Manganese sulfate
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU823402661A SU1127545A1 (en) | 1982-02-18 | 1982-02-18 | Nutrient medium for growing hyacinth in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU823402661A SU1127545A1 (en) | 1982-02-18 | 1982-02-18 | Nutrient medium for growing hyacinth in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU1127545A1 true SU1127545A1 (en) | 1984-12-07 |
Family
ID=20999493
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU823402661A SU1127545A1 (en) | 1982-02-18 | 1982-02-18 | Nutrient medium for growing hyacinth in vitro |
Country Status (1)
| Country | Link |
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| SU (1) | SU1127545A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696464A (en) * | 2012-06-15 | 2012-10-03 | 江苏沿江地区农业科学研究所 | Osmanthus branch hydroponic rooting method by using nutrient solution |
-
1982
- 1982-02-18 SU SU823402661A patent/SU1127545A1/en active
Non-Patent Citations (1)
| Title |
|---|
| 1. Коблиц X. Культура изолированных органов, тканей и клеток растений. М., 1970, с. 192-197. 2. Tidsskrift for Plantearl. 1971, № 3, p. 391. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696464A (en) * | 2012-06-15 | 2012-10-03 | 江苏沿江地区农业科学研究所 | Osmanthus branch hydroponic rooting method by using nutrient solution |
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