SK1452003A3 - Tartrate salts of thiazolidinedione derivative - Google Patents

Abstract

A novel pharmaceutical compound 5-[4-2-(N-methyl-N-(2-pyridyl)amino)ethoxy]benzyl]thiazolidine-2,4-dione Meso- Tartrate or a solvate thereof, a process for preparing such a compound, a pharmaceutical composition comprising such a compound and the use of such a compound in medicine.

Description

THIAZOLIDINIDIONE DERIVATIVE IN THE FORM OF TARTRIC ACID SALT

Technical field

The present invention relates to a novel medicament, to a method of manufacture of a medicament and to the use of a medicament in medicine.

BACKGROUND OF THE INVENTION

European Patent Application Publication No. 0,306,228 relates to certain thiazolidinedione derivatives in which hypoglycemic and hypolipidemic activity has been described. The compound of Example 30 of EP 0 306 228 is 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione (hereinafter also referred to as compound ( I)).

International Patent Application Publication No. WO94 / 05659 discloses certain salts of the compounds of EP 0 306 228, one of which is a tartaric acid salt, tartrate. A preferred salt of WO94 / 05659 is a maleic acid salt.

SUMMARY OF THE INVENTION

It has now been found tartrate (listed below in mesotartrate).

The novel mesotartrate is a high melting point, free-flowing material and accessible to pharmaceuticals especially in the production processes that Compound (I) forms a novel herein also referred to as a stable crystalline substance and is therefore suitable for preparing its transport. Mesotartrate is a large-scale processing that requires or produces heat, such as grinding, fluidized bed drying, spray drying, hot grinding, and autoclave sterilization. The mesotartrate may also be prepared by an efficient, economical and reproducible process particularly suitable for large-scale preparation.

The novel mesotartrate also has useful pharmaceutical properties and has been shown to be useful in the treatment and / or prophylaxis of diabetes mellitus, disease states associated with diabetes mellitus and certain complications thereof.

Accordingly, the present invention provides 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidine-2,4-dione in the form of mesotartrate or a solvate thereof.

Preferably, the mesotartrate is a monotartrate.

The monotartrates also optionally include salts of other monovalent ions such as an alkali metal ion or an ammonium cation.

In one preferred aspect, the mesotartrate infrared spectrum substantially according to Figure 1

In one preferred aspect, the mesotartrate is a Raman spectrum substantially according to Figure 2.

In one preferred aspect, the mesotartrate powder diffractogram (XRPD) substantially according to or Figure 3.

Providers Provides Tables 1

In one preferred aspect, the mesotartrate provides the solid phase C NMR spectrum of substantially Figure 4.

In one preferred aspect, the mesotartrate provides a melting point in the range of 147-157 ° C, such as

148-155 ° C, e.g. 148 ° C, 153 ° C and 155 ° C.

In a preferred aspect, the invention provides 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione in the form of a mesotartrate characterized by providing:

(i) substantially infrared spectrum of Figure 1; and (ii) essentially Raman spectrum of Figure 2; and (iii) a powder diffractogram (XRPD) substantially of Table 1 or Figure 3; and (iv) a solid state substantially ¹³ C NMR spectrum. as shown.

The present invention encompasses the mesotartrate or solvate thereof isolated in pure form or mixed with other substances. Thus, in one aspect, the mesotartrate or solvate thereof is provided in isolated form.

In another aspect, the mesotartrate or solvate thereof is provided in purified form.

In yet another aspect, the mesotartrate or solvate thereof is provided in crystalline form.

The invention also provides the mesotartrate or solvate thereof in a solid pharmaceutically acceptable form, such as a solid dosage form, especially when adapted for oral administration.

In addition, the invention also provides the mesotartrate or solvate thereof in a pharmaceutically acceptable form, particularly in free-flowing form, such form being particularly suitable for pharmaceutical processing, especially in production processes that require or produce heat, for example grinding, e.g. whirling or spray drying, for example hot milling treatment, for example heat sterilization, such as autoclave sterilization.

In addition, the invention provides the mesotartrate or solvate thereof in a pharmaceutically acceptable form, in particular in free-flowing form, and in particular in a form requiring or producing heat, for example in milled form, for example in heat-dried form, in particular spray-dried form, for example in the form of a heat-milled form, for example in a heat-sterilized form, such as autoclave sterilization.

A suitable solvate is a hydrate.

The invention also provides a process for the preparation of mesotartrate or a solvate thereof, characterized in that 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidine-2,4-dione (compound (I) l) or a salt thereof, preferably dispersed or dissolved in a suitable solvent, is reacted with a source of mesotartrate ion, and then, if desired, a solvate of the resulting mesotartrate is prepared and the mesotartrate or solvate thereof is obtained.

A suitable reaction solvent is a ketone such as acetone or an ether such as tetrahydrofuran, an alkanol such as propan-2-ol, a hydrocarbon such as toluene, an ester such as ethyl acetate, a nitrile such as acetonitrile, or a halogenated hydrocarbon , such as dichloromethane, or water or an organic acid, such as acetic acid, or mixtures thereof.

Typically, the mesotartrate ion source is mesotartaric acid. The mesotartaric acid is preferably added as a solid or in solution, for example in water or a lower alcohol such as methanol, ethanol or propan-2ol or in a solvent mixture. An alternative mesotartrate ion source is provided by a suitable soluble basic meso tartaric salt, such as ammonium mesotartrate, or a meso tartaric acid amine salt, such as ethylamine or diethylamine.

The concentration of compound (I) is preferably in the range of 2 to 25% w / v, more preferably in the range of 5 to 20%. The tartaric acid concentration of the solutions is preferably in the range of 5 to 125% w / v.

The reaction is usually carried out at room temperature or at elevated temperature, for example at the reflux temperature of the solvent, although any suitable temperature may be used to provide the desired product.

Mesotartrate solvates, such as hydrates, are prepared according to conventional procedures.

Recovery of the desired compound generally involves crystallization from a suitable solvent or mixture of solvents, usually a reaction solvent, usually in the presence of cooling. For example, the mesotartrate may be crystallized from a ketone such as acetone or an ether such as tetrahydrofuran or water or mixtures thereof. A better salt yield can be obtained by evaporating some or all of the solvents or by crystallization at elevated temperature followed by controlled cooling, stages. Careful temperature control can be used to improve preferably after precipitation of the reproducibility of the product form.

Crystallization can also be initiated by seeding with mesotartrate crystals or a solvate thereof, but this is not essential.

When the monotartrate contains salts of other monovalent ions, such as an alkali metal ion or an ammonium cation, the ion is usually formed by reacting the monotartrate with a solution of a selected monovalent salt ion, such as a metal ion or an ammonium ion. Alternatively, compound (I) may be treated with a monovalent salt of a monovalent ion.

Compound (I) is prepared according to known procedures, such as those described in EP 0 306 228 and WO94 / 05659. The descriptions of EP 0 306 228 and WO94 / 05659 are incorporated herein by reference.

The mesotartaric acid is a commercially available compound.

When used herein, the term T _initial is generally determined by differential scanning calorimetry and has the meaning commonly understood in the art, as exemplified in "Pharmaceutical Thermal Analysis, Techniques and Applications, Ford and Timmins, 1989 as the temperature corresponding to the intersection of the baseline before the transitional stage with the extrapolated front edge of the transitional stage.

When used herein, the term "good flow properties related to certain compounds" is appropriately characterized in that the compound has a Hausner coefficient of less than or equal to 1.5, in particular less than or equal to 1.25.

The Hausner coefficient is an accepted term in the field.

As used herein, the term "prophylaxis of conditions associated with diabetes mellitus" includes treatment of diseases such as insulin resistance, impaired glucose tolerance, hyperinsulinemia, and gestational diabetes.

Diabetes mellitus preferably means Type II diabetes mellitus.

Diseases associated with diabetes include hyperglycemia and insulin resistance and obesity. Other conditions associated with diabetes include hypertension, cardiovascular disease, in particular atherosclerosis, some eating disorders, particularly the regulation of appetite and food intake in patients suffering from eating disorders such as anorexia nervosa and eating disorders such as obesity and anorexia bulimia. Other disease states associated with diabetes include polycystic ovarian syndrome and steroid-induced insulin resistance.

Complications of the disease states associated with diabetes mellitus included herein include kidney disease, particularly kidney disease associated including type II diabetes glomerulonephritis, diabetic disease developing nephropathy, glomerulosclerosis, nephrotic syndrome, hypertensive nephrosclerosis, and end stage renal disease.

As mentioned above, the compound of the invention has useful therapeutic properties, the present invention accordingly provides mesotartrate or a solvate thereof for use as an active therapeutic agent.

More specifically, the present invention provides mesotartrate or a solvate thereof for use in the treatment and / or prophylaxis of diabetes mellitus, disease states associated with diabetes mellitus and certain complications thereof.

The mesotartrate or solvate thereof may be administered per se or preferably as a pharmaceutical composition also comprising a pharmaceutically acceptable carrier. Suitable methods for formulating the mesotartrate or solvate thereof are generally those described for the compound publications.

’I) in the above

Accordingly, the present invention also provides a pharmaceutical composition comprising mesotartrate or a solvate thereof and a pharmaceutically acceptable carrier therefor.

The mesotartrate or solvate thereof is normally administered in unit dosage form.

The active compound may be administered by any suitable route, but usually orally or parenterally. For such use, the compound is normally used in the form of a pharmaceutical composition in association with a pharmaceutical carrier, diluent and / or excipient, although the exact form of the composition will naturally depend on the mode of administration.

The formulations are prepared by admixture and are suitably adapted for oral, parenteral or topical administration, and as such may be in the form of tablets, capsules, oral liquid preparations, powders, granules, lozenges, lozenges, reconstitutable powders, solutions or suspensions for injection and infusion. , suppositories, and transdermal devices. Formulations for oral administration are preferred, in particular shaped oral formulations, since they are more convenient for general use.

Tablets and capsules for oral administration are usually presented in a unit dose and contain conventional excipients such as binding agents, fillers, diluents, tabletting agents, lubricants, disintegrants, colorants, flavoring agents and wetting agents. The tablets may be coated according to methods well known in the art.

Fillers suitable for use include cellulose, mannitol, lactose and other similar agents. Suitable disintegrants include starch, polyvinylpyrrolidone and starch derivatives such as sodium starch glycolide. Suitable lubricants include, for example, magnesium stearate. Suitable pharmaceutically acceptable wetting agents include sodium lauryl sulfate.

Solid oral preparations may be prepared by conventional methods of mixing, filling, tabletting and the like. Repeated blending processes can be used to distribute the active ingredient everywhere in formulations that use large quantities of fillers. However, such processes are common in the art.

Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions, emulsions, syrups or elixirs, or may be presented as a dry product for reconstitution with water or other suitable vehicle before use. Such liquid preparations may contain conventional additives such as suspending agents, for example sorbitol, syrup, methylcellulose, gelatin, hydroxyethylcellulose, carboxymethylcellulose, aluminum gel stearate or hydrogenated edible fats, emulsifiers, for example lecithin, sorbitan monooleate or acacia, which may include edible oils) such as almond oil, fractionated coconut oil, oily esters such as glycerine esters, propylene glycol or ethyl alcohol, preservatives such as methyl or propyl p-hydroxybenzoate or sorbic acid and, if desired, conventional flavoring or coloring agents.

For parenteral administration, liquid unit dosage forms are prepared comprising a compound of the present invention and a sterile vehicle. The compound, depending on the vehicle and concentration, can be either suspended or dissolved. Parenteral solutions are normally prepared by dissolving the active compound in a vehicle and sterilizing by filtration prior to filling into suitable vials or ampoules and sealing. Preferably, they are also dissolved in the vehicle with adjuvants such as local anesthetics, preservatives, and buffering agents. To enhance stability, the formulation can be frozen after filling into vials and the water removed under vacuum.

Parenteral suspensions are prepared in substantially the same manner except that the active compound is suspended in the vehicle instead of being dissolved and sterilized by exposure to ethylene oxide prior to suspension in the sterile vehicle. Preferably, a surfactant or wetting agent is included in the formulation to facilitate uniform distribution of the active compound.

As is common practice, the compositions will usually be accompanied by written or printed instructions for use in the respective medicament treatment.

As used herein, the term "pharmaceutically acceptable includes compounds, compositions and ingredients for both human and veterinary use: for example, the term" pharmaceutically acceptable salt "includes a veterinarily acceptable salt.

The present invention further provides a method of treating and / or prophylaxis of diabetes mellitus, a disease condition associated with diabetes mellitus and certain complications thereof, in a human or non-human mammal which comprises administering to a human or non-human mammal an effective nontoxic amount of mesotartrate or solvate thereof. who needs this treatment e.

Usually, the active ingredient may be administered as a pharmaceutical composition as defined hereinabove and this constitutes a particular aspect of the present invention.

In another aspect, the present invention provides the use of mesotartrate or a solvate thereof for the manufacture of a medicament for the treatment and / or prophylaxis of diabetes mellitus, disease states associated with diabetes mellitus and certain complications thereof.

In the treatment and / or prophylaxis of diabetes mellitus, disease states associated with diabetes mellitus and certain complications thereof, the mesotartrate or solvate thereof may be taken in an amount to provide compound (I) at appropriate doses such as those described in EP 0 306 228, WO94 / 05659 or WO98 / 55122.

The compounds of the invention in the above treatment did not show any adverse toxicological effects.

The following examples illustrate the invention but do not limit it in any way.

DETAILED DESCRIPTION OF THE INVENTION

Example 1

5- [4- [2- (N-Methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione as mesotartrate

A mixture of 8.0 g of 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione and 160 ml of tetrahydrofuran was stirred and heated to 50 ° C. . A solution of 3.84 g of tartaric acid in 20 ml of water was added and the mixture was stirred at 50 ° C for 15 minutes, filtered and the clear filtrate was cooled to 21 ° C. The solvent was evaporated under reduced pressure at 40 ° C, 40 mL of acetone was added and the mixture was stirred at 21 ° C to give a white suspension. The product was collected by filtration, washed with acetone and dried under vacuum to give 10.6 g of 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidine-2,4-dione as mesotartrate as a white crystalline solid.

Example 2

5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione as mesotartrate

A mixture of 3.0 g (8.4 mmol) of 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione, 40 ml of acetone and 5 ml of tetrahydrofuran was heated to reflux for 2.5 hours with stirring. To this mixture was added a solution of 1.41 g (8.4 mmol) of tartaric acid monohydrate in 2 mL of water. The reaction mixture was heated to reflux with stirring for 2.5 hours, then cooled to 21 ° C and stirred for 16 hours at 21 ° C. The white solid was collected by filtration, washed with 40 mL in acetone, and then dried under reduced pressure for 2.5 hours at 21 ° C to give 3.25 g of 5- [4- [2- (N-methyl-N-) (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione as mesotartrate as a white crystalline solid.

Example 3

5- [4- [2- (N-Methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione as mesotartrate

A mixture of 10.0 g (28 mmol) of 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione, 120 mL of acetone and 15 mL of tetrahydrofuran was heated. to reflux for 50 minutes while stirring. To this mixture was added a solution of 4.7 g (28 mmol) of tartaric acid monohydrate in 6.0 mL of water. The reaction mixture was heated to reflux with stirring for 1 hour, then cooled to 21 ° C and stirred for 16 hours at 21 ° C. The white solid was collected by filtration, washed with 50 mL, and then dried under reduced pressure for 3 hours at 21 ° C to give 11.7 g of 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) (ethoxy) benzyl] thiazolidine-2,4-dione as mesotartrate as a white crystalline solid.

Characteristic data recorded for the product of Example 1

The infrared absorption spectrum of the product dispersion in mineral oil was obtained using a spectrometer

Nicolet 710 FT-IR with a resolution of 2 cm ¹ (Figure 1). Data were digitized at intervals of 1 „-1 cm. belts were observed at: 3405, 1738, 1694, 1629, 1556 1537, 1505 1459 1436 1418, 1330, 1268, 1249, 1223 , 1182, 1167, 1143, 1104 1057, 1033, 1000, 949, 916 887, 853, 818 771.

717, 668, 619, 568, 528,515 cm ^-1 .

The solid product infrared spectrum was recorded using a Perkin-Elmer Spectrum One FT-IR spectrometer equipped with universal ATR accessories. The bands were observed at: 3407, 2937, 2750, 1739, 1693, 1628, 1555, 1535, 1504, 1476, 1459, 1412, 1388, 1358, 1330, 1261, 1248, 1222, 1182, 1167, 1143, 1101, 1074 1056, 1033, 999, 948, 916, 887, 853, 818, 771, 744, 717, 667 cm ^first

The Raman spectrum of the product (Figure 2) was recorded with a sample in an NMR tube using a Nicolet 960 ESPFT-Raman spectrometer at 4 cm ¹ excitation from a Nd: V04 laser (1064 nm) with an output power of 400mW. The bands were obserbed in: 3101, 3059, 2952, 2922, 2877, 1739, 1604, 1544, 1458, 1438, 1394, 1331, 1270, 1222, 1203, 979, 887, 827, 739, 668, 638, 605, 472 , 344 cm ¹ .

The powder diffractogram of the product (Figure 3) was recorded using the following collection conditions: Tube anode: Cu, Generator voltage: 40 kV,

Generator current: 40 mA, starting angle: 2.0 ° 2D, final angle: 35.0 ° 2D, step size: 0.02 ° 2Q, step time: 2.5 seconds. The characteristic XRPD angles and relative intensities are shown in Table 1.

Table 1

angle Rel. intensity 20 ° ABOUT. about 4.9 11.4 9.3 12.4 9/9 3, 1 12.2 1, 9 14.3 3.3 14.9 7 15.2 19 15, 9 23.8 16, 2 13.9 17.0 WHAT 17.3 17.5 18.1 25.9 18.5 31.3 19.1 10.7 19, 9 18.4 20.4 100 21, 6 26 22.2 23.7 22.7 6.1

23, 6 23.1 24.5 5.7 25.2 23 25.8 9.2 26.2 17.8 26.8 11.5 27.4 14.7 28.1 6.8 28.4 8.7 29.7 5.7 30.2 9.7 30.6 9.3 31.1 23.6 31.4 13.4 32.2 14 32.6 19.7 32.9 15.2 33.6 11.4 33.8 12.4 34.4 8.7 34.7 9

The solid-state NMR spectrum of the product (Figure 4) was recorded on a Bruker AMX360 operating at 90.55 MHz: The solid was loaded into a 4 mm Kel-F zirconium MAS rotor and the rotor was rotated at approximately 10 kHz. ^{The 13} C MAS spectrum was obtained by cross-polarization of protons according to Hartmann-Hahn condition (CP contact time 3 ms, repetition time 15 s) and protons were decapped during harvesting using a modulated phase series of two composite pulses (TPPM). Chemical shifts were externally related to the carboxylate signal of glycine at 176.4 ppm relative to TMS and were observed at: 183.0,

177.2, 175.4, 173.0, 160.0, 159.0, 151.8, 144.2, 138.9,

134.3, 130.8, 128.2, 123.6, 113.7, 112.8, 77.4, 76.7,

74.5, 55.5, 53.5, 50.0, 42.4, 36.6, 33.8 ppm.

Properties of mesotartrate

Solid phase stability of mesotartrate recorded for the product of Example 1

The solid phase stability of the drug substance was determined by storing approximately 1.0 g of the substance in a glass vial (vi) 40 ° C / 75% relative humidity (RH), open for 1 month and b) at 50 ° C, closed for 1 month .

In both cases, the substance was tested by HPLC to determine the final content and degradation products.

a) 40 ° C / 75% RH: no significant degradation was observed (HPLC assay 97% starting).

b) 50 ° C: no significant degradation was observed (HPLC assay 98% starting).

Melting point of mesotartrate

The melting point of mesotartrate was determined according to the method described in the US Pharmacopoeia, USP 23, 1995, <741> Melting range or temperature, Procedure for Class 1a, using a Buchi melting temperature measuring apparatus.

545th

The product of Example 1,

The product of Example 2,

The product of Example 3,

Thirty-one mesotartrate

temperature mp: 149 ° C temperature mp: 153 ° C temperature mp: 155 ° C

The initial drug substance was determined by differential scanning calorimetry using a Perkin-Elmer DSC.

The product of Example 1, T eye _count (10 ° C / minute, closed drum): 146 ° C,

The product of example 2, T _POCl eye (10 ° C / minute, open drum): 153 ° C

The product of Example 3, T _initial (10 ° C / minute, open drum: 154 ° C)

Claims (12)

PATENT CLAIMS A compound 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy) benzyl] thiazolidin-2,4-dione in the form of mesotartrate or a solvate thereof. A compound according to claim 1 which provides: (i) an infrared spectrum substantially as in Figure 1; (ii) a Raman spectrum substantially as in Figure 2; (iii) a powder diffractogram (XRPD) substantially as in Table 1 or Figure 3; or (iv) a solid state ¹³ C NMR spectrum. according to the picture 4. The compound of claim 1, which provides two or more of the following: (i) an infrared spectrum substantially according to Figure 1; and (ii) a Raman spectrum substantially according to Figure 2; and (iii) a powder diffractogram (XRPD) substantially according to Table 1 or Figure 3; and (iv) a solid state ¹³ C NMR spectrum. according to the picture 4. A compound according to any one of claims 1 to 3 in purified form. A compound according to any one of claims 1 to 3 in solid dosage form. Compound according to any one of claims 1 to 3, in a form suitable for pharmaceutical processing in a production process that requires or produces heat, for example grinding, for example heat drying, in particular drying in a fluidized bed or spray drying, for example heat milling processing, for example sterilization heat, such as autoclave sterilization. A compound according to any one of claims 1 to 3 in a form suitable for pharmaceutical processing in a production process that requires or produces heat, for example in a milled form, for example in a heat-dried form, in particular in a fluidized bed or spray-dried form. for example in the form of a heat milling process, for example in a heat sterilized form such as autoclave sterilization. A compound according to any one of claims 1 to 3 in a pharmaceutically acceptable form having good flow properties. A process for the preparation of mesotartrate or a solvate thereof, characterized in that 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidine-2,4-dione (compound (1)) or a salt thereof is reacted with a source of mesotartrate ion and then, if desired, a solvate of the resulting mesotartrate is prepared and the mesotartrate or solvate thereof is obtained. A pharmaceutical composition comprising 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidine-2,4-dione in the form of mesotartrate or a solvate thereof; and a pharmaceutically acceptable carrier. The compound 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidine-2,4-dione in the form of mesotartrate or a solvate thereof for use as an active therapeutic substance. Use of 5- [4- [2- (N-methyl-N- (2-pyridyl) amino) ethoxy] benzyl] thiazolidin-2,4-dione in the form of mesotartrate or a solvate thereof for the manufacture of a medicament for the treatment and / or prophylaxis of diabetes mellitus, disease states associated with diabetes mellitus and certain complications thereof.