SK129198A3 - Purification of pharmaceutical-grade plasmid dna - Google Patents
Purification of pharmaceutical-grade plasmid dna Download PDFInfo
- Publication number
- SK129198A3 SK129198A3 SK1291-98A SK129198A SK129198A3 SK 129198 A3 SK129198 A3 SK 129198A3 SK 129198 A SK129198 A SK 129198A SK 129198 A3 SK129198 A3 SK 129198A3
- Authority
- SK
- Slovakia
- Prior art keywords
- dna
- plasmid
- sequence
- buffer
- column
- Prior art date
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- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
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- 125000004432 carbon atom Chemical group C* 0.000 description 1
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- 229920000126 latex Polymers 0.000 description 1
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- 210000004962 mammalian cell Anatomy 0.000 description 1
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- 238000005374 membrane filtration Methods 0.000 description 1
- 230000011987 methylation Effects 0.000 description 1
- 238000007069 methylation reaction Methods 0.000 description 1
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical group CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
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- 230000020477 pH reduction Effects 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000004713 phosphodiesters Chemical class 0.000 description 1
- 150000008039 phosphoramides Chemical class 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
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- 230000000717 retained effect Effects 0.000 description 1
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- 239000000377 silicon dioxide Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- YZHUMGUJCQRKBT-UHFFFAOYSA-M sodium chlorate Chemical compound [Na+].[O-]Cl(=O)=O YZHUMGUJCQRKBT-UHFFFAOYSA-M 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 231100000378 teratogenic Toxicity 0.000 description 1
- 230000003390 teratogenic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 239000010891 toxic waste Substances 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- AVBGNFCMKJOFIN-UHFFFAOYSA-N triethylammonium acetate Chemical compound CC(O)=O.CCN(CC)CC AVBGNFCMKJOFIN-UHFFFAOYSA-N 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 231100000925 very toxic Toxicity 0.000 description 1
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- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9603519A FR2746412B1 (fr) | 1996-03-21 | 1996-03-21 | Purification d'adn plasmidique de qualite pharmaceutique |
PCT/FR1997/000472 WO1997035002A1 (fr) | 1996-03-21 | 1997-03-17 | Purification d'adn plasmidique de qualite pharmaceutique |
Publications (1)
Publication Number | Publication Date |
---|---|
SK129198A3 true SK129198A3 (en) | 1999-03-12 |
Family
ID=9490391
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SK1291-98A SK129198A3 (en) | 1996-03-21 | 1997-03-17 | Purification of pharmaceutical-grade plasmid dna |
Country Status (15)
Country | Link |
---|---|
US (1) | US6730781B1 (fr) |
EP (1) | EP0902835A1 (fr) |
JP (1) | JP2000506736A (fr) |
KR (1) | KR100502116B1 (fr) |
AU (1) | AU730755B2 (fr) |
BR (1) | BR9708227A (fr) |
CA (1) | CA2249465A1 (fr) |
CZ (1) | CZ295587B6 (fr) |
FR (1) | FR2746412B1 (fr) |
HU (1) | HU225426B1 (fr) |
IL (1) | IL126268A0 (fr) |
NO (1) | NO984342D0 (fr) |
SK (1) | SK129198A3 (fr) |
WO (1) | WO1997035002A1 (fr) |
ZA (1) | ZA972462B (fr) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7026468B2 (en) | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
US7807822B2 (en) | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
US6214586B1 (en) * | 1997-12-08 | 2001-04-10 | Genzyme Corporation | Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA |
SK287662B6 (sk) * | 2000-05-26 | 2011-05-06 | Centelion | Spôsob purifikácie dvojvláknovej DNA použitím imobilizovaného oligonukleotidu |
FR2822476B1 (fr) * | 2001-03-23 | 2004-04-02 | Aventis Pharma Sa | Procedes de purification et de detection de sequences cibles d'adn double brin par interaction triple helice |
MXPA03008642A (es) * | 2001-03-23 | 2004-06-30 | Gencell Sa | Metodos de purificacion y de deteccion de secuencias objetivo de adn de doble hebra mediante interaccion de triple helice. |
EP1554398A4 (fr) | 2002-09-13 | 2005-12-14 | Valentis Inc | Appareil et procede de purification a l'echelle de la chimie preparatoire d'acides nucleiques |
US7238522B2 (en) | 2003-05-30 | 2007-07-03 | Advisys, Inc. | Devices and methods for biomaterial production |
CN1882682B (zh) * | 2003-09-17 | 2010-06-16 | 森特利昂公司 | 药物级别质粒dna的制备方法 |
EP2246413A3 (fr) * | 2004-04-19 | 2011-10-26 | Centelion | Procédé de préparation d'ADN plasmide de qualité pharmaceutique |
CA2559368A1 (fr) * | 2004-04-19 | 2005-10-27 | Centelion | Procede destine a purifier de l'adn plasmidique |
MX2007001847A (es) | 2004-08-16 | 2007-04-13 | Nature Technology Corp | Cepas mejoradas de e.coli para la produccion de adn de plasmido. |
EP1817423B1 (fr) | 2004-11-30 | 2015-01-07 | Merial Ltd. | Melangeurs pour chimiolyse de cellules |
US20120258502A1 (en) * | 2011-04-08 | 2012-10-11 | Vinod Pandiripally | Method of producing recombinant plasmid dna using substantially solid growth medium |
US20120283503A1 (en) * | 2011-04-29 | 2012-11-08 | The Johns Hopkins University | Nanoparticle loaded stem cells and their use in mri guided hyperthermia |
CN116790578B (zh) * | 2023-08-22 | 2023-12-12 | 赛奥斯博生物科技(北京)有限公司 | 一种基于碱裂解法快速纯化质粒的生产工艺 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2691586B2 (ja) * | 1987-10-22 | 1997-12-17 | 旭光学工業株式会社 | 多孔質セラミックス及びその製法 |
JPH01135792A (ja) * | 1987-11-20 | 1989-05-29 | Mitsui Toatsu Chem Inc | 核酸の分離・精製方法 |
CA2006008C (fr) * | 1988-12-20 | 2000-02-15 | Donald J. Kessler | Methode pour obtenir des oligonucleotides synthetiques qui se lient specifiquement a des sites cibles sur des molecules d'adn double brin, en formant un triplex colineaire; methode d'utilisation de ces oligonucleotides synthetiques |
WO1994016075A2 (fr) * | 1993-01-13 | 1994-07-21 | Genetics Institute, Inc. | Procede de production de m-csf 223 |
US5503816A (en) * | 1993-09-27 | 1996-04-02 | Becton Dickinson And Company | Silicate compounds for DNA purification |
DE59505786D1 (de) * | 1994-02-07 | 1999-06-02 | Qiagen Gmbh | Verfahren zur abreicherung oder entfernung von endotoxinen |
US5576196A (en) * | 1995-01-13 | 1996-11-19 | Vical Incorporated | Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth |
EP0880536B2 (fr) | 1996-02-06 | 2015-08-05 | Roche Diagnostics GmbH | Procede pour la preparation d'acide nucleique purifie et utilisation de ce dernier |
-
1996
- 1996-03-21 FR FR9603519A patent/FR2746412B1/fr not_active Expired - Fee Related
-
1997
- 1997-03-17 IL IL12626897A patent/IL126268A0/xx unknown
- 1997-03-17 BR BR9708227A patent/BR9708227A/pt not_active Application Discontinuation
- 1997-03-17 HU HU9902152A patent/HU225426B1/hu not_active IP Right Cessation
- 1997-03-17 KR KR10-1998-0707410A patent/KR100502116B1/ko not_active IP Right Cessation
- 1997-03-17 SK SK1291-98A patent/SK129198A3/sk unknown
- 1997-03-17 CA CA002249465A patent/CA2249465A1/fr not_active Abandoned
- 1997-03-17 WO PCT/FR1997/000472 patent/WO1997035002A1/fr not_active Application Discontinuation
- 1997-03-17 JP JP9533199A patent/JP2000506736A/ja active Pending
- 1997-03-17 CZ CZ19982984A patent/CZ295587B6/cs not_active IP Right Cessation
- 1997-03-17 EP EP97914411A patent/EP0902835A1/fr not_active Ceased
- 1997-03-17 AU AU21661/97A patent/AU730755B2/en not_active Ceased
- 1997-03-20 ZA ZA9702462A patent/ZA972462B/xx unknown
-
1998
- 1998-09-15 US US09/153,838 patent/US6730781B1/en not_active Expired - Fee Related
- 1998-09-18 NO NO984342A patent/NO984342D0/no not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
BR9708227A (pt) | 1999-07-27 |
HUP9902152A2 (hu) | 1999-11-29 |
NO984342L (no) | 1998-09-18 |
CZ298498A3 (cs) | 1999-01-13 |
ZA972462B (en) | 1997-09-29 |
AU730755B2 (en) | 2001-03-15 |
FR2746412A1 (fr) | 1997-09-26 |
FR2746412B1 (fr) | 1998-06-12 |
WO1997035002A1 (fr) | 1997-09-25 |
EP0902835A1 (fr) | 1999-03-24 |
IL126268A0 (en) | 1999-05-09 |
CZ295587B6 (cs) | 2005-08-17 |
KR20000064693A (ko) | 2000-11-06 |
CA2249465A1 (fr) | 1997-09-25 |
KR100502116B1 (ko) | 2005-12-20 |
HUP9902152A3 (en) | 2001-04-28 |
JP2000506736A (ja) | 2000-06-06 |
HU225426B1 (en) | 2006-11-28 |
US6730781B1 (en) | 2004-05-04 |
NO984342D0 (no) | 1998-09-18 |
AU2166197A (en) | 1997-10-10 |
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