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SI23754A - Protection of color, coloring and antioxidative and anti-microbial protection of meat and meat products - Google Patents

Protection of color, coloring and antioxidative and anti-microbial protection of meat and meat products Download PDF

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SI23754A
SI23754A SI201100247A SI201100247A SI23754A SI 23754 A SI23754 A SI 23754A SI 201100247 A SI201100247 A SI 201100247A SI 201100247 A SI201100247 A SI 201100247A SI 23754 A SI23754 A SI 23754A
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extract
meat
mixture
protection
acid
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SI201100247A
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Slovenian (sl)
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Kolar Majda Hadolin
Simona URBANČIČ
Duška DIMITRIJEVIĆ
NIDERL Maša HOJNIK
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Vitiva D.D.
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Priority claimed from SI201000260A external-priority patent/SI23469A/en
Priority claimed from SI201100037A external-priority patent/SI23629A/en
Application filed by Vitiva D.D. filed Critical Vitiva D.D.
Priority to SI201100247A priority Critical patent/SI23754A/en
Priority to PCT/IB2011/001985 priority patent/WO2012028928A2/en
Publication of SI23754A publication Critical patent/SI23754A/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B4/00Preservation of meat, sausages, fish or fish products
    • A23B4/14Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
    • A23B4/18Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
    • A23B4/20Organic compounds; Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00Preservation of foods or foodstuffs, in general
    • A23B2/70Preservation of foods or foodstuffs, in general by treatment with chemicals
    • A23B2/725Preservation of foods or foodstuffs, in general by treatment with chemicals in the form of liquids or solids
    • A23B2/729Organic compounds; Microorganisms; Enzymes
    • A23B2/733Compounds of undetermined constitution obtained from animals or plants

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita maščob in maščobo-vsebujočih živil, predvsem mesa in mesnih izdelkov po izumu je izvedena z dodatkom sinergistične mešanice aktivnih učinkovin na osnovi rastlin družine ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil, prednostno ekstrakta rdeče pese ter opcijsko za izboljšanje antimikrobiološke učinkovitosti z dodatkom ekstrakta zelenega čaja in/ali ekstrakta olivnih listov in/ali ekstrakta citrusov in/ali ekstraktahmelja in/ali ekstrakta korenja. Naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, je opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenegačaja in/ali EDTA. Stabilizirana mešanica barvil se lahko uporabi tudi samostojno za barvanje mesa kot nadomestek karminske kisline in karmina. Za izboljšanje porazdelitve po mesu ali mesnih izdelkih je sinergistični mešanici aktivnih učinkovin dodana sol, maltodekstrin, dekstroza ali drugi v prehrani dovoljen nosilec in/ali v prehrani dovoljen emulgator in/ali v prehrani dovoljeno sredstvo proti strjevanju. Sinergistična mešanica vsebuje 0-50 odstotkov karnozolne kisline in/ali 0-50 odstotkov rožmarinskekisline, 0-30 odstotkov askorbinske kisline, 0-20 odstotkov antocianov in/ali 0-5 odstotkov betaninov. Mesu in mesnim izdelkom se doda mešanica v takšnem doziranju, da je skupna koncentracija aktivnih učinkovin (karnozolne kisline in/ali rožmarinske kisline in/ali askorbinske kisline in/ali antocianov in/ali betanina) v mesu ali mesnem izdelku vsaj 1 ppm. Zaščiteno meso in mesni izdelki ohranijo svežo, rožnato barvo, teksturo, okus in svež vonj.Color protection, coloring and antioxidant and antimicrobial protection of fats and fat-containing foods, especially meat and meat products according to the invention is carried out by adding a synergistic mixture of active ingredients based on plants of the labiate family and/or acerola extract and/or natural red dyes, preferably beetroot extract, and optionally to improve antimicrobial efficacy by adding green tea extract and/or olive leaf extract and/or citrus extract and/or hop extract and/or carrot extract. The natural red dye, preferably beetroot extract, is optionally pre-added with paprika oleoresin and/or carrot extract and/or turmeric extract and/or safflower extract, after which the dye mixture is stabilized by adding rosemary extract and/or CITREM emulsifier and/or citric acid and/or ascorbic acid and/or acerola extract and/or rosehip extract and/or tocopherols (mixed or individual isomers) and/or lemon extract and/or green tea extract and/or EDTA. The stabilized dye mixture can also be used alone for dyeing meat as a substitute for carminic acid and carmine. To improve the distribution in meat or meat products, salt, maltodextrin, dextrose or another food-approved carrier and/or food-approved emulsifier and/or food-approved anti-caking agent is added to the synergistic active ingredient mixture. The synergistic mixture contains 0-50 percent carnosic acid and/or 0-50 percent rosmarinic acid, 0-30 percent ascorbic acid, 0-20 percent anthocyanins and/or 0-5 percent betanins. The mixture is added to meat and meat products in such a dosage that the total concentration of active ingredients (carnosic acid and/or rosmarinic acid and/or ascorbic acid and/or anthocyanins and/or betanin) in the meat or meat product is at least 1 ppm. The protected meat and meat products retain their fresh, pink color, texture, taste and fresh smell.

Description

ZAŠČITA BARVE, BARVANJE TER ANTIOKSIDATIVNA IN ANTIMIKROBIOLOŠKA ZAŠČITA MESA IN MESNIH IZDELKOVPAINT PROTECTION, PAINTING AND ANTIOXIDATIVE AND ANTIMICROBIOLOGICAL PROTECTION OF MEAT AND MEAT PRODUCTS

Predmet izuma je zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov z mešanico ekstrakta rastlin družine ustnatic in/ali ekstrakta acerole in/ali naravnega rdečega barvila, prednostno ekstrakta rdeče pese ter opcijsko za izboljšanje antimikrobiološke učinkovitosti z dodatkom ekstrakta zelenega čaja in/ali ekstrakta olivnih listov in/ali ekstrakta citrusov in/ali ekstrakta hmelja in/ali ekstrakta korenja. Kombinacija ekstraktov namreč sinergistično deluje in daje boljšo zaščito barve ter antioksidativno in antimikrobiološko zaščito izdelka. Naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, je opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenega čaja in/ali EDTA. Stabilizirana mešanica barvil se lahko uporabi tudi samostojno za barvanje mesa kot nadomestek karminske kisline in karmina.The subject of the invention is the protection of color, dyeing and antioxidant and antimicrobial protection of meat and meat products with a mixture of plant extract of the oral family and / or acerola extract and / or natural red colorant, preferably red beet extract, and optionally for improving the antimicrobial efficiency by adding green extract and / or olive leaf extract and / or citrus and / or hop extract and / or carrot extract. The combination of extracts works synergistically and gives better color protection and antioxidant and antimicrobial protection of the product. Natural red colorant, preferably red beet extract, is optionally pre-added with pepper oleoresin and / or carrot extract and / or turmeric extract and / or safflower extract, after which the colorant mixture is stabilized by the addition of CITREM citrus / rosemary / emulsifier or CITREM emulsifier and / or ascorbic acid and / or acerola extract and / or pomegranate and / or tocopherol extracts (mixed or single isomers) and / or lemon and / or green tea extract and / or EDTA. A stabilized dye mixture can also be used independently to dye meat as a substitute for carminic acid and carmine.

Ustnatice so rožmarin, žajbelj, melisa, origano, šetraj, meta, timijan in druge. Glavne aktivne učinkovine v ekstraktih rastlin družine ustnatic so kamozolna kislina, kamozol, rožmarinska kislina in njihovi derivati. Kamozolna kislina in kamozol sta oljetopni, rožmarinska kislina pa vodotopna aktivna učinkovina. Oljetopni ekstrakt rastlin družine ustnatic vsebuje od 0-100 ut.% kamozolne kisline in 0-20 ut.% kamozola. Vodotopni ekstrakt rastlin družine ustnatic vsebuje 0-100 ut.% rožmarinske kisline.Lips are rosemary, sage, lemon balm, oregano, walleye, mint, thyme and more. The main active substances in the extracts of the plants of the oral family are camosolic acid, camosole, rosemary acid and their derivatives. Camosoleic acid and camosole are oil-soluble, while rosemary acid is a water-soluble active substance. The lipid extract of the plants of the lipid family contains from 0-100% by weight of camosolic acid and 0-20% by weight of camosole. The water-soluble plant extract of the lipid family contains 0-100% by weight of rosemary acid.

Glavna antioksidativne učinkovina v ekstraktu acerole je askorbinska kislina. Na tržišču so ekstrakti z različno vsebnostjo askorbinske kisline, običajno od 17 do 30 %.The main antioxidant ingredient in acerola extract is ascorbic acid. Extracts with different ascorbic acid content, usually from 17 to 30%, are commercially available.

K naravnim rdečim barvilom spadajo antociani, izolirani iz različnih rastlinskih materialov in betanini, izolirani iz rdeče pese.Natural red dyes include anthocyanins isolated from various plant materials and betanins isolated from red beets.

V okviru izuma se kot meso štejejo vse vrste svežega mesa in kot izdelki vsi produkti mesa.In the context of the invention, all types of fresh meat are considered as meat and all meat products.

• ·• ·

-2Meso in mesni izdelki so podvrženi spremembi barve, mikrobiološkemu kvarjenju in oksidaciji maščob, kar se kaže v spremembi okusa, vonja in barve. Hkrati izgubijo hranilno vrednost. Da bi preprečili oz. upočasnili proces kvarjenja se razen termičnih postopkov kot antioksidativna in/ali mikrobiološka sredstva uporabljajo različni sintetični aditivi: askorbinska kislina in njene soli, izoaskorbinska kislina in njene soli, natrijev acetat in drugi. Alternativo sintetičnim predstavljajo naravna antioksidativna in/ali antimikrobiološka sredstva, ki imajo naslednje prednosti: uporaba po principu quantum satis, 100 % naravni, GRAS status. V okviru izuma je predstavljena zaščita barve ter barvanje mesa in mesnih izdelkov s sinergistično mešanico na osnovi ekstrakta rastlin družine ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil, prednostno ekstrakta rdeče pese. Naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, je opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenega čaja in/ali EDTA. Stabilizirana mešanica barvil se lahko uporabi tudi samostojno za barvanje mesa kot nadomestek karminske kisline in karmina.-2 Meat and meat products are subject to discoloration, microbial deterioration and fat oxidation, which results in a change in taste, odor and color. At the same time, they lose their nutritional value. In order to prevent or slow down the deterioration process, apart from thermal processes, various synthetic additives are used as antioxidant and / or microbiological agents: ascorbic acid and its salts, iso-ascorbic acid and its salts, sodium acetate and others. An alternative to synthetic is natural antioxidant and / or antimicrobial agents that have the following advantages: quantum satis principle, 100% natural, GRAS status. The present invention provides for the protection of color and the coloring of meat and meat products with a synergistic mixture based on plant extract of the lipid family and / or acerola extract and / or natural red dyes, preferably red beet extract. Natural red colorant, preferably red beet extract, is optionally pre-added with pepper oleoresin and / or carrot extract and / or turmeric extract and / or safflower extract, after which the colorant mixture is stabilized by the addition of CITREM citrus / rosemary / emulsifier or CITREM emulsifier and / or ascorbic acid and / or acerola extract and / or pomegranate and / or tocopherol extracts (mixed or single isomers) and / or lemon and / or green tea extract and / or EDTA. A stabilized dye mixture can also be used independently to dye meat as a substitute for carminic acid and carmine.

Glavne aktivne učinkovine v ekstraktih rastlin družine ustnatic so kamozolna kislina, kamozol, rožmarinska kislina in njihovi derivati. Antioksidativno delujejo kot donorji vodikovega atoma. Glavna antioksidativna učinkovina v ekstraktu acerole je askorbinska kislina, glavne aktivne učinkovine rdečih barvil pa so antocianini in betanin v ekstraktu rdeče pese.The main active substances in the extracts of the plants of the oral family are camosolic acid, camosole, rosemary acid and their derivatives. Antioxidants act as donors to the hydrogen atom. The main antioxidant ingredient in acerola extract is ascorbic acid, and the main active ingredients in red dyes are anthocyanins and betanin in red beet extract.

Naloga in cilj izuma je zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov s sinergistično mešanico aktivnih učinkovin na osnovi ekstraktov rastlin družine ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil, prednostno ekstrakta rdeče pese ter opcijsko za izboljšanje antimikrobiološke učinkovitosti z dodatkom ekstrakta zelenega čaja in/ali ekstrakta olivnih listov in/ali ekstrakta citrusov in/ali ekstrakta hmelja in/ali ekstrakta korenja. Naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, je opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenega čaja in/ali EDTA. Stabilizirana mešanica barvil se lahko uporabi tudi samostojno za barvanje mesa kot nadomestek karminske kisline in karmina.The object and object of the invention is the protection of color, dyeing and antioxidant and antimicrobial protection of meat and meat products with a synergistic mixture of active ingredients based on plant extracts of the oral family and / or acerola extract and / or natural red dyes, preferably red beet extract and optionally for improving antimicrobial efficacy by the addition of green tea extract and / or olive leaf extract and / or citrus extract and / or hops extract and / or carrot extract. Natural red colorant, preferably red beet extract, is optionally pre-added with pepper oleoresin and / or carrot extract and / or turmeric extract and / or safflower extract, after which the colorant mixture is stabilized by the addition of CITREM citrus / rosemary / emulsifier or CITREM emulsifier and / or ascorbic acid and / or acerola extract and / or pomegranate and / or tocopherol extracts (mixed or single isomers) and / or lemon and / or green tea extract and / or EDTA. A stabilized dye mixture can also be used independently to dye meat as a substitute for carminic acid and carmine.

• ·• ·

-3Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov se lahko uporabi v kombinaciji z vsemi možnimi pakiranji, kot so: normalno pakiranje, pakiranje v dvojno folijo (vacuum skin packaging), aktivno pakiranje, aktivno pakiranje z dodatki za uravnavanje kisline, pakiranje v folijo z dodatkom zaščitnih novi, pakiranje z vsemi oblikami modificirane atmosfere, pakiranje v kontrolirano atmosfero in drugi.-3 Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products can be used in combination with all possible packages, such as: normal packaging, vacuum skin packaging, active packaging, active packaging with acid regulation additives , packaging in foil with the addition of protective new, packaging with all forms of modified atmosphere, packaging in controlled atmosphere and others.

Po izumu je naloga rešena z zaščito po neodvisnem patentnem zahtevku.According to the invention, the problem is solved by protection according to an independent patent claim.

Izum bo opisan z izvedbenimi primeri.The invention will be described by way of example examples.

Po izumu je naloga rešena z zaščito barve, barvanjem ter antioksidativno in antimikrobiološko zaščito mesa in mesnih izdelkov z dodatkom sinergistične mešanice aktivnih učinkovin na osnovi ekstrakta ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil, pri čemer mešanica vsebuje od 0-50 % kamozolne kisline in/ali 0-50 % rožmarinske kisline, 0-30 % askorbinske kisline, 0-20 % antocianov in/ali 0-5 % betaninov. Naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, je opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenega čaja in/ali EDTA. Stabilizirana mešanica barvil se lahko uporabi tudi samostojno za barvanje mesa kot nadomestek karminske kisline in karmina.According to the invention, the problem is solved by color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products with the addition of a synergistic mixture of active substances based on lipstick extract and / or acerola extract and / or natural red dyes, the mixture containing from 0-50% camosolic acids and / or 0-50% rosemary acid, 0-30% ascorbic acid, 0-20% anthocyanins and / or 0-5% betanins. Natural red colorant, preferably red beet extract, is optionally pre-added with pepper oleoresin and / or carrot extract and / or turmeric extract and / or safflower extract, after which the colorant mixture is stabilized by the addition of CITREM citrus / rosemary / emulsifier or CITREM emulsifier and / or ascorbic acid and / or acerola extract and / or pomegranate and / or tocopherol extracts (mixed or single isomers) and / or lemon and / or green tea extract and / or EDTA. A stabilized dye mixture can also be used independently to dye meat as a substitute for carminic acid and carmine.

Opcijsko se lahko za izboljšanje antimikrobiološke učinkovitosti mešanici doda ekstrakt zelenega čaja in/ali ekstrakt olivnih listov in/ali ekstrakt citrusov in/ali ekstrakt hmelja in/ali ekstrakt korenja, pri čemer ekstrakt zelenega čaja vsebuje 0-100 % epigalokatehin galata, ekstrakt olivnih listov vsebuje 0-100 % oleuropeina, ekstrakt citrusov vsebuje 0-100 % skupnih citrusovih bioflavonoidov, ekstrakt hmelja vsebuje 0-100 % alfa in beta kislin ter ekstrakt korenja vsebuje falcarindiol kot aktivno učinkovino. Mešanici se lahko doda jedilna sol, maltodextrin, dekstroza ali drugi v prehrani dovoljen nosilec in/ali v prehrani dovoljen emulgator in/ali v prehrani dovoljeno sredstvo proti strjevanju, kar je smiselno z vidika boljše porazdelitve v mesu in mesnih izdelkih.Optionally, green tea extract and / or olive leaf extract and / or citrus extract and / or hops extract and / or carrot extract may be added to the mixture to improve antimicrobial efficacy, the green tea extract containing 0-100% epigallocatechin gallate, olive leaf extract contains 0-100% oleuropein, citrus extract contains 0-100% total citrus bioflavonoids, hops extract contains 0-100% alpha and beta acids, and carrot extract contains falcarindiol as active ingredient. To the mixture may be added edible salt, maltodextrin, dextrose or other dietary acceptable carrier and / or diet-approved emulsifier and / or diet-approved anti-caking agent, which makes sense in terms of better distribution in meat and meat products.

Z dodatkom omenjene sinergistične mešanice aktivnih učinkovin zaščiteno meso in mesni izdelki ohranijo svežo, rožnato barvo, teksturo, okus in svež vonj.By adding the aforementioned synergistic mixture of active ingredients, protected meat and meat products retain a fresh, pink color, texture, taste and a fresh aroma.

-4Sinergistično mešanico aktivnih učinkovin na osnovi ekstrakta ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil v meso in mesne izdelke ter ribe in ribje izdelke dodamo v takšni koncentraciji, da je skupna koncentracija aktivnih učinkovin (kamozolne kisline in/ali rožmarinske kisline in/ali askorbinske kisline in/ali antocianov in/ali betanina) v končnem izdelku vsaj 1 ppm (mg/kg).-4 A synergistic mixture of active substances based on lipstick extract and / or acerola extract and / or natural red dyes in meat and meat products and fish and fish products is added at a concentration such that the total concentration of the active substances (camosolic acids and / or rosemary acids and / or ascorbic acid and / or anthocyanins and / or betanine) in the finished product at least 1 ppm (mg / kg).

Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov se lahko uporabi v kombinaciji z vsemi možnimi pakiranji, kot so: normalno pakiranje, pakiranje v dvojno folijo (vacuum skin packaging), aktivno pakiranje, aktivno pakiranje z dodatki za uravnavanje kisline, pakiranje v folijo z dodatkom zaščitnih novi, pakiranje z vsemi oblikami modificirane atmosfere, pakiranje v kontrolirano atmosfero in drugi.Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products can be used in combination with all possible packaging such as: normal packaging, vacuum skin packaging, active packaging, active packaging with acid regulation additives, packaging in foil with the addition of protective new, packaging with all forms of modified atmosphere, packaging in controlled atmosphere and others.

PRIMER 1EXAMPLE 1

Ekstrakt acerole, ki je vseboval 25 ut. % askorbinske kisline, smo v koncentraciji 0,2 ut.% vmešali v mleto goveje meso. Hkrati smo pripravili vzorec brez dodatkov. Oba vzorca sta bila pripravljena v treh paralelkah. Vzorci so bili hranjeni v plastičnih vrečkah v hladilniku na temperaturi 4°C. Vzorce smo fotografirali s fotoaparatom Canon 350 D digital po proizvodnji, ter po 2, 6, 8 in 10 dneh hranjenja.Acerol extract containing 25 wt. % ascorbic acid was mixed in minced beef at a concentration of 0.2% by weight. At the same time, we prepared a sample without additives. Both samples were prepared in triplicate. The samples were stored in plastic bags in a refrigerator at 4 ° C. Samples were taken with a Canon 350 D digital camera after production and after 2, 6, 8 and 10 days of storage.

Po 2 dneh hranjenja je meso pri vzorcu brez dodatkov že izgubilo sveže rdečo barvo, pri vzorcu z dodatkom 0,2 ut. % ekstrakta acerole pa se je barva mesa ohranila do 6. dneva hranjenja. Vzorec z dodatkom 0,2 ut. % ekstrakta acerole je podaljšal svežo barvo mesa za 3-krat v primerjavi s kontrolnim vzorcem brez antioksidantov.After 2 days of feeding, the meat had already lost a fresh red color to the sample with no additives, with 0.2 wt. % of acerola extract, however, remained the color of the meat until day 6 of feeding. Sample with addition of 0,2 wt. % of acerola extract prolonged the fresh meat color by 3-fold compared to the control without antioxidants.

PRIMER 2EXAMPLE 2

Rožmarinov ekstrakt, ki je vseboval 20 ut. % kamozolne kisline, smo zmešali z ekstraktom acerole, ki je vseboval 25 ut. % askorbinske kisline. Sinergistično mešanico (MEŠANICA 1) smo dobro zmešali in zmleli. Končna koncentracija aktivnih komponent v mešanici je bila 1 ut. % kamozolne kisline in 18,75 ut. % askorbinske kisline.Rosemary extract containing 20 wt. % of camosolic acid was mixed with acerola extract containing 25 wt. % ascorbic acid. The synergistic mixture (MIXTURE 1) was mixed well and ground. The final concentration of active components in the mixture was 1 wt. % of camosolic acid and 18.75 wt. % ascorbic acid.

Mešanico MEŠANICA 1 smo v koncentraciji 0,3 ut. % vmešali v mleto goveje meso. Hkrati smo pripravili vzorec z dodatkom 0,2 ut. % ekstrakta acerole in vzorec brez dodatkov. Vsi trije vzorci so bili pripravljeni v treh paralelkah. Hranili smo jih v plastičnih vrečkah v hladilniku na temperaturi 4°C. Vzorce smo fotografirali s fotoaparatom Canon 350 D digital in izmerili TBA število po proizvodnji, ter po 2, 6, 8 in 10 dneh hranjenja. Mikrobiološka ocena vzorcev je bila narejena po 10 dneh hranjenja.The mixture MIXTURE 1 is in a concentration of 0.3 wt. % mixed in ground beef. At the same time, a sample was prepared with the addition of 0.2 wt. % of acerola extract and sample without additives. All three samples were prepared in triplicate. They were stored in plastic bags in a refrigerator at 4 ° C. Samples were photographed using a Canon 350 D digital camera and TBA counts were measured after production and after 2, 6, 8, and 10 days of storage. Microbiological evaluation of the samples was made after 10 days of feeding.

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-5Ρο 2 dneh hranjenja je meso pri vzorcu brez dodatkov že izgubilo sveže rdečo barvo, pri vzorcu z dodatkom 0,2 ut, % ekstrakta acerole se je barva mesa ohranila do 6. dneva hranjenja, po 8 dneh hranjenja pa je bilo meso delno sivo.-5Ρο 2 days of feeding, the meat had already lost a fresh red color in the sample with no additives, the color of the meat was preserved with the addition of 0.2 wt% acerola extract until the 6th day of feeding, and after 8 days of feeding the meat was partially gray .

Pri vzorcu z dodatkom 0,3 ut. % mešanice MEŠANICA 1 seje barva mesa ohranila do 6. dneva hranjenja, po 8. dneh hranjenja je meso rahlo izgubilo svežino barve.For a sample with the addition of 0.3 wt. % of the mixture MIXTURE 1 session the color of the meat remained until the 6th day of feeding, after 8 days of feeding the meat slightly lost its freshness.

Vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 1 je podaljšal svežo barvo mesa za 15 % v primerjavi vzorcem z dodatkom 0,2 ut.% ekstrakta acerole in za 4-krat v primerjavi z vzorcem brez dodatkov.Sample with addition of 0,3 wt. % of MIXTURE 1 lengthened the fresh color of the meat by 15% compared to samples with the addition of 0.2% by weight of acerola extract and 4 times compared with the sample without additives.

Hkrati seje vzorec z dodatkom MEŠANICE 1 glede na merjenje oksidativnih sprememb (TBA število, ki meri koncentracijo malonaldehida kot sekundarnega produkta oksidacije) in skupnega števila mikroorganizmov pokazal kot oksidativno in mikrobiološko stabilnejši v primerjavi z vzorcem brez dodatkov.At the same time, the sample, with the addition of MIXTURE 1, proved to be oxidatively and microbiologically more stable compared to the sample without additives with respect to the measurement of oxidative changes (TBA number, which measures the concentration of malonaldehyde as a secondary oxidation product) and the total number of microorganisms.

PRIMER 3EXAMPLE 3

Rožmarinov ekstrakt, ki je vseboval 20 ut.% kamozolne kisline, smo zmešali z ekstraktom acerole, ki je vseboval 25 ut.% askorbinske kisline in ekstraktom rdeče pese, ki je vseboval 2,5 ut.% betanina. Sinergistično mešanico (MEŠANICA 2) smo dobro zmešali in zmleli. Končna koncentracija aktivnih komponent v mešanici je bila 1 ut. % kamozolne kisline, 17,12 ut. % askorbinske kisline in 0,16 ut. % betanina.Rosemary extract containing 20% by weight of camosolic acid was mixed with acerola extract containing 25% by weight ascorbic acid and beetroot extract containing 2.5% by weight betanin. The synergistic mixture (MIXTURE 2) was mixed well and ground. The final concentration of active components in the mixture was 1 wt. % of camosolic acid, 17.12 wt. % ascorbic acid and 0.16 wt. % betanine.

Mešanico MEŠANICA 2 smo v koncentraciji 0,2 ut. % vmešali v mleto goveje meso. Hkrati smo pripravili vzorec z dodatkom 0,2 ut. % ekstrakta acerole, vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 1 (glej Primer 2) in vzorec brez dodatkov. Vsi vzorci so bili pripravljeni v treh paralelkah. Hranili smo jih v plastičnih vrečkah v hladilniku na temperaturi 4°C. Vzorce smo fotografirali s fotoaparatom Canon 350 D digital in izmerili TBA število po proizvodnji, ter po 2, 6, 8 in 10 dneh hranjenja. Mikrobiološka ocena vzorcev je bila narejena po 10 dneh hranjenja.The mixture MIXTURE 2 is at a concentration of 0.2 wt. % mixed in ground beef. At the same time, a sample was prepared with the addition of 0.2 wt. % of acerola extract, sample with the addition of 0,3 wt. % of mixture MIXTURE 1 (see Example 2) and sample without additives. All samples were prepared in triplicate. They were stored in plastic bags in a refrigerator at 4 ° C. Samples were photographed using a Canon 350 D digital camera and TBA counts were measured after production and after 2, 6, 8, and 10 days of storage. Microbiological evaluation of the samples was made after 10 days of feeding.

Po 2 dneh hranjenja je meso pri vzorcu brez dodatkov že izgubilo sveže rdečo barvo, pri vzorcu z dodatkom 0,2 ut. % ekstrakta acerole se je barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,3 ut. % mešanice MEŠANICA 1 seje barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je meso rahlo izgubilo svežino barve. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 2 pa se je barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo.After 2 days of feeding, the meat had already lost a fresh red color to the sample with no additives, with 0.2 wt. % of acerola extract, the color of the meat remained until day 6 of feeding and after 8 days of feeding the meat was partially gray. For a sample with the addition of 0.3 wt. % of the mixture MIXTURE 1 session the color of the meat remained until the 6th day of feeding and after 8 days of feeding the meat slightly lost its freshness. In the sample with the addition of 0.2 wt. % of the MIXTURE 2 mixture, however, the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray.

-6Vzoreč z dodatkom 0,2 ut. % mešanice MEŠANICA 2 je podaljšal svežo barvo mesa za 10 % v primerjavi vzorcem z dodatkom 0,3 ut. % mešanice MEŠANICA 1, za 33 % v primerjavi z vzorcem z dodatkom 0,2 ut. % ekstrakta acerole in za 4,5-krat v primerjavi z vzorcem brez dodatkov.-6Sample with 0.2 wt. % of the mixture MIXTURE 2 prolonged the fresh color of the meat by 10% compared to the samples with the addition of 0.3% % of the mixture MIXTURE 1, by 33% compared to the sample with the addition of 0.2% by weight % acerola extract and 4.5-fold compared to the sample without additives.

Hkrati sta se vzorca z dodatkom mešanic MEŠANICA 1 in MEŠANICA 2 glede na merjenje oksidativnih sprememb (TBA število, ki meri koncentracijo malonaldehida kot sekundarnega produkta oksidacije) in skupnega števila mikroorganizmov pokazala kot oksidativno in mikrobiološko stabilnejša v primerjavi z vzorcem brez dodatkov.At the same time, the samples with the addition of MIXTURE 1 and MIXTURE 2, with respect to the measurement of oxidative changes (TBA number, which measures the concentration of malonaldehyde as a secondary product of oxidation) and the total number of microorganisms proved to be more oxidatively and microbiologically more stable compared to the sample without additives.

PRIMER 4EXAMPLE 4

Rožmarinov ekstrakt, ki je vseboval 80 ut. % rožmarinske kisline, smo zmešali z ekstraktom acerole, ki je vseboval 25 ut.% askorbinske kisline in ekstraktom rdeče pese, ki je vseboval 2,5 ut.% betanina. Sinergistično mešanico (MEŠANICA 3) smo dobro zmešali in zmleli. Končna koncentracija aktivnih komponent v mešanici je bila 1,50 ut. % rožmarinske kisline, 22,90 ut. % askorbinske kisline in 0,16 ut. % betanina.Rosemary extract containing 80 wt. of rosemary acid was mixed with acerola extract containing 25% by weight ascorbic acid and red beet extract containing 2.5% by weight betanin. The synergistic mixture (MIXTURE 3) was mixed well and ground. The final concentration of the active components in the mixture was 1.50 wt. % rosemary acid, 22,90 wt. % ascorbic acid and 0.16 wt. % betanine.

Mešanico MEŠANICA 3 smo v koncentraciji 0,2 ut.% vmešali v mleto goveje meso. Hkrati smo pripravili vzorec z dodatkom 0,2 ut. % ekstrakta acerole, vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 1, vzorec z dodatkom 0.2 ut. % mešanice MEŠANICA 2 in vzorec brez dodatkov. Vsi vzorci so bili pripravljeni v treh paralelkah. Vzorci so bili hranjeni v plastičnih vrečkah v hladilniku na temperaturi 4°C. Vzorce smo fotografirali s fotoaparatom Canon 350 D digital in izmerili TBA število po proizvodnji, ter po 2, 6, 8 in 10 dneh hranjenja. Mikrobiološka ocena vzorcev je bila narejena po 10 dneh hranjenja.The Mixture 3 mixture was mixed in minced beef at a concentration of 0.2% by weight. At the same time, a sample was prepared with the addition of 0.2 wt. % of acerola extract, sample with the addition of 0,3 wt. % of the mixture MIXTURE 1, sample with addition of 0.2 wt. % of mixture MIXTURE 2 and sample without additives. All samples were prepared in triplicate. The samples were stored in plastic bags in a refrigerator at 4 ° C. Samples were photographed using a Canon 350 D digital camera and TBA counts were measured after production and after 2, 6, 8, and 10 days of storage. Microbiological evaluation of the samples was made after 10 days of feeding.

Po 2. dneh hranjenja je meso pri vzorcu brez dodatkov že izgubilo sveže rdečo barvo, pri vzorcu z dodatkom 0,2 ut. % ekstrakta acerole se je barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,3 ut.% mešanice MEŠANICA 1 se je barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je meso rahlo izgubilo svežino barve. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 2 se je barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 3 se je barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo.After 2 days of feeding, the meat had already lost a fresh red color to the sample with no additives, with 0.2 wt. % of acerola extract, the color of the meat remained until day 6 of feeding and after 8 days of feeding the meat was partially gray. In the sample with the addition of 0.3% by weight of the MIXTURE 1 mixture, the color of the meat remained until the 6th day of feeding and after 8 days of feeding the meat slightly lost its freshness. In the sample with the addition of 0.2 wt. % of the MIXTURE 2 mixture, the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray. In the sample with the addition of 0.2 wt. % of the MIXTURE 3 mixture, the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray.

Vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 3 je deloval enako kot vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 2, kar pomeni, da vodotopna rožmarinska kislina in oljetopna kamozolna kislina v kombinaciji z ekstraktom acerole in ekstraktom rdeče pese v enaki meriSample with addition of 0,2 wt. % of the mixture MIXTURE 3 worked the same as the sample with the addition of 0.2 wt. % of the mixture MIXTURE 2, which means that the water-soluble rosemary acid and the oil-soluble camosolic acid in combination with acerola extract and red beet extract are equally

-7ohranjata barvo svežega mesa. Vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 3 je ohranil svežo barvo mesa za 10 % v primerjavi vzorcem z dodatkom 0,3 ut. % mešanice MEŠANICA 1, za 33 % v primerjavi z vzorcem z dodatkom 0,2 ut. % ekstrakta acerole in za 4,5 krat v primerjavi z vzorcem brez dodatkov.-7 preserve the color of fresh meat. Sample with addition of 0,2 wt. % of the mixture MIXTURE 3 kept the meat color fresh by 10% compared to the samples with the addition of 0.3 wt. % of the mixture MIXTURE 1, by 33% compared to the sample with the addition of 0.2% by weight % acerola extract and 4.5 times the sample without additives.

Hkrati so se vzorci z dodatkom mešanic MEŠANICA 1, MEŠANICA 2 in MEŠANICA 3 glede na merjenje oksidativnih sprememb (TBA število, ki meri koncentracijo malonaldehida kot sekundarnega produkta oksidacije) in skupnega števila mikroorganizmov pokazali kot oksidativno in mikrobiološko stabilnejši v primerjavi z vzorcem brez dodatkov.At the same time, with the addition of MIXTURE 1, MIXTURE 2 and MIXTURE 3, the samples showed oxidative and microbial stability as oxidative and microbiologically more stable compared to the non-additive sample with respect to the measurement of oxidative changes (TBA number, which measures the concentration of malonaldehyde as a secondary oxidation product).

PRIMER 5EXAMPLE 5

Ekstrakt rdeče pese je bil stabiliziran z dodatkom rožmarinovega ekstrakta, kije vseboval 20 % kamozolne kisline, rožmarinovega ekstrakta, ki je vseboval 15 % rožmarinske kisline, z dodatkom emulgatorja CITREM (citratni estri mono- in digliceridov maščobnih kislin), oleorezina paprike in ekstraktom acerole. Sinergistično mešanico (MEŠANICA 4) smo dobro zmešali in zmleli. Končna koncentracija aktivnih komponent v mešanici je bila 0,15 ut. % rožmarinske kisline, 0,20 ut. % kamozolne kisline 0,75 ut. % askorbinske kisline, 1,00 ut. % emulgatorja CITREM, 2,50 ut % oleorezina paprike in 0,3 ut. % betanina.Beetroot extract was stabilized by the addition of rosemary extract containing 20% camosolic acid, rosemary extract containing 15% rosemary acid, with the addition of the CITREM emulsifier (citrate esters of mono- and diglycerides of fatty acid acrylic and extralerocyric acid). The synergistic mixture (MIXTURE 4) was mixed well and ground. The final concentration of the active components in the mixture was 0.15 wt. % rosemary acid, 0.20 wt. % of camosolic acid 0.75 wt. % ascorbic acid, 1.00 wt. % CITREM emulsifier, 2.50 wt% pepper oleoresin and 0.3 wt. % betanine.

Mešanico MEŠANICA 4 smo v koncentraciji 0,1 ut.% vmešali v mleto goveje meso. Hkrati smo pripravili vzorec z dodatkom 0,2 ut. % ekstrakta acerole, vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 1, vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 2, vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 3 in vzorec brez dodatkov. Vsi vzorci so bili pripravljeni v treh paralelkah. Vzorci so bili hranjeni v plastičnih vrečkah v hladilniku na temperaturi 4°C. Vzorce smo fotografirali s fotoaparatom Canon 350 D digital in izmerili TBA število po proizvodnji, ter po 2, 6, 8, 9 in 10 dneh hranjenja. Mikrobiološka ocena vzorcev je bila narejena po 10 dneh hranjenja.The Mixture 4 mixture was mixed in minced beef at a concentration of 0.1% by weight. At the same time, a sample was prepared with the addition of 0.2 wt. % of acerola extract, sample with the addition of 0,3 wt. % of the mixture MIXTURE 1, sample with 0.2 wt. % of the mixture MIXTURE 2, sample with 0.2 wt. % of MIXTURE 3 mixture and sample without additives. All samples were prepared in triplicate. The samples were stored in plastic bags in a refrigerator at 4 ° C. Samples were photographed using a Canon 350 D digital camera and TBA counts were measured after production and after 2, 6, 8, 9, and 10 days of storage. Microbiological evaluation of the samples was made after 10 days of feeding.

Po 2 dneh hranjenja je meso pri vzorcu brez dodatkov že izgubilo sveže rdečo barvo, pri vzorcu z dodatkom 0,2 ut. % ekstrakta acerole se je barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,3 ut.% mešanice MEŠANICA 1 se je barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je meso rahlo izgubilo svežino barve. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 2 se je barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 3 seje barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,1 ut. % mešaniceAfter 2 days of feeding, the meat had already lost a fresh red color to the sample with no additives, with 0.2 wt. % of acerola extract, the color of the meat remained until day 6 of feeding and after 8 days of feeding the meat was partially gray. In the sample with the addition of 0.3% by weight of the MIXTURE 1 mixture, the color of the meat remained until the 6th day of feeding and after 8 days of feeding the meat slightly lost its freshness. In the sample with the addition of 0.2 wt. % of the MIXTURE 2 mixture, the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray. In the sample with the addition of 0.2 wt. % of the mixture MIXTURE 3 sessions the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray. In the sample with the addition of 0,1 wt. % of the mixture

-8MEŠANICA 4 se je barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo.-8MIXTURE 4, the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray.

Vzorec z dodatkom 0,1 ut. % mešanice MEŠANICA 4 je deloval enako kot vzorca z dodatkom mešanic MEŠANICA 2 in MEŠANICA 3, kar pomeni, da stabiliziran ekstrakt rdeče pese, brez dodatka acerolinega ekstrakta in vodotopnega/oljetopnega rožmarinovega ekstrakta v enaki meri ohranja barvo svežega mesa kot mešanice acerolinega ekstrakta, vodotopnega/oljetopnega rožmarinovega ekstrakta in nestabiliziranih betaninov. , Vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 4 je ohranil svežo barvo mesa za 10 % v primerjavi z vzorcem z dodatkom 0,3 ut. % mešanice MEŠANICA 1, , za 33 % v primerjavi z vzorcem z dodatkom 0,2 ut. % ekstrakta acerole in za 4,5-krat v primerjavi z vzorcem brez dodatkov.Sample with addition of 0,1 wt. % of MIXTURE 4 mixture worked the same as the sample with the addition of MIXTURE 2 and MIXTURE 3, which means that the stabilized red beet extract, without the addition of aceroline extract and water-soluble / oil-derived rosemary extract, retains the color of fresh meat equally as the mixture of acero-mixtures / Rosemary oil extract and unstabilized betanines. , Sample with addition of 0.2 wt. % of the mixture MIXTURE 4 maintained a fresh meat color by 10% compared to the sample with the addition of 0.3 wt. % of the mixture MIXTURE 1,, by 33% compared to the sample with the addition of 0.2% by weight. % acerola extract and 4.5-fold compared to the sample without additives.

Hkrati so se vzorci z dodatkom mešanic MEŠANICA 1, MEŠANICA 2, MEŠANICA 3 in MEŠANICA 4 glede na merjenje oksidativnih sprememb (TBA število, ki meri koncentracijo malonaldehida kot sekundarnega produkta oksidacije) in skupnega števila mikroorganizmov pokazali kot oksidativno in mikrobiološko stabilnejši v primerjavi z vzorcem brez dodatkov. Podobno učinkovitost smo dobili, če smo namesto ekstrakta acerole k ekstraktu rdeče pese dodali askorbinsko kislino, preračunano na enako količino aktivne snovi.At the same time, with the addition of Mixtures 1, Mixture 2, Mixture 3 and Mixture 4, the samples showed oxidative and microbial stability compared to the sample with respect to the measurement of oxidative changes (TBA number, which measures the concentration of malonaldehyde as a secondary oxidation product) and the total number of microorganisms. without accessories. Similar efficacy was obtained by adding ascorbic acid, calculated on the same amount of active substance, to the red beet extract instead of acerola extract.

PRIMER 6EXAMPLE 6

Rožmarinov ekstrakt, ki je vseboval 80 ut. % rožmarinske kisline, smo zmešali z ekstraktom acerole, ki je vseboval 25 ut.% askorbinske kisline in ekstraktom rdeče pese, ki je vseboval 0,3 ut.% betanina. Ekstrakt rdeče pese je bil stabiliziran z dodatkom EDTA, oleorezina paprike in ekstraktom acerole. Sinergistično mešanico (MEŠANICA 5) smo dobro zmešali in zmleli. Končna koncentracija aktivnih komponent v mešanici je bila 1,00 ut. % rožmarinske kisline, 15,50 ut. % askorbinske kisline, 0,36 ut. % EDTA, 0,86 ut % oleorezina paprike, in 0,11 ut. % betanina.Rosemary extract containing 80 wt. of rosemary acid was mixed with acerola extract containing 25% by weight ascorbic acid and red beet extract containing 0.3% by weight betanin. Beetroot extract was stabilized by the addition of EDTA, pepper oleoresin, and acerola extract. The synergistic mixture (MIXTURE 5) was mixed well and ground. The final concentration of active components in the mixture was 1.00 wt. % rosemary acid, 15.50 wt. % ascorbic acid, 0.36 wt. % EDTA, 0.86 wt% pepper oleoresin, and 0.11 wt. % betanine.

Mešanico MEŠANICA 5 smo v koncentraciji 0,3 ut.% vmešali v mleto goveje meso. Hkrati smo pripravili vzorec z dodatkom 0,2 ut. % ekstrakta acerole, vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 1, vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 2, vzorec z dodatkom 0,2 ut. % mešanice MEŠANICA 3, vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 4 in vzorec brez dodatkov. Vsi vzorci so bili pripravljeni v treh paralelkah. Vzorci so bili hranjeni v plastičnih vrečkah v hladilniku na temperaturi 4°C. Vzorce smo fotografirali s fotoaparatom Canon 350 D digital in izmerili TBA število po proizvodnji, ter po 2, 6, 8, 9 in 10 dneh hranjenja. Mikrobiološka ocena vzorcev je bila narejena po 10 dneh hranjenja.The MIXTURE 5 mixture was mixed in minced beef at a concentration of 0.3% by weight. At the same time, a sample was prepared with the addition of 0.2 wt. % of acerola extract, sample with the addition of 0,3 wt. % of the mixture MIXTURE 1, sample with 0.2 wt. % of the mixture MIXTURE 2, sample with 0.2 wt. % of the mixture MIXTURE 3, sample with the addition of 0.3 wt. % of MIXTURE 4 mixture and sample without additives. All samples were prepared in triplicate. The samples were stored in plastic bags in a refrigerator at 4 ° C. Samples were photographed using a Canon 350 D digital camera and TBA counts were measured after production and after 2, 6, 8, 9, and 10 days of storage. Microbiological evaluation of the samples was made after 10 days of feeding.

-9Ρο 2 dneh hranjenja je meso pri vzorcu brez dodatkov že izgubilo sveže rdečo barvo, pri vzorcu z dodatkom 0,2 ut. % ekstrakta acerole seje barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,3 ut. % mešanice MEŠANICA 1 seje barva mesa ohranila do 6. dneva hranjenja in po 8 dneh hranjenja je meso rahlo izgubilo svežino barve. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 2 se je barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,2 ut. % mešanice MEŠANICA 3 seje barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,1 ut. % mešanice MEŠANICA 4 seje barva mesa ohranila do 8. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo. Pri vzorcu z dodatkom 0,3 ut. % mešanice MEŠANICA 5 se je barva mesa ohranila do 9. dneva hranjenja in po 10 dneh hranjenja je bilo meso delno sivo.-9Ρο 2 days of feeding, the meat has already lost a fresh red color to the sample with no additives, with 0.2 wt. % acerole extract of the session the color of the meat remained until day 6 of feeding and after 8 days of feeding the meat was partially gray. For a sample with the addition of 0.3 wt. % of the mixture MIXTURE 1 session the color of the meat remained until the 6th day of feeding and after 8 days of feeding the meat slightly lost its freshness. In the sample with the addition of 0.2 wt. % of the MIXTURE 2 mixture, the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray. In the sample with the addition of 0.2 wt. % of the mixture MIXTURE 3 sessions the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray. In the sample with the addition of 0,1 wt. % of the mixture MIXTURE 4 sessions the color of the meat remained until day 8 of feeding and after 10 days of feeding the meat was partially gray. For a sample with the addition of 0.3 wt. % of the MIXTURE 5 mixture, the color of the meat remained until day 9 of feeding and after 10 days of feeding the meat was partially gray.

Vzorec z dodatkom 0,3 ut. % mešanice MEŠANICA 5 je ohranil barvo svežega mesa za 13 % v primerjavi z vzorcem z dodatkom 0,1 ut. % mešanice MEŠANICA 4, za 13 % v primerjavi z vzorcem z dodatkom 0,2 ut. % mešanice MEŠANICA 3, za 13 % v primerjavi z vzorcem z dodatkom 0,2 ut. % mešanice MEŠANICA 2, za 29 % v primerjavi vzorcem z dodatkom 0,3 ut. % mešanice MEŠANICA 1, za 1,5 krat v primerjavi z vzorcem z dodatkom 0,2 ut. % ekstrakta acerole in za 5 krat v primerjavi z vzorcem brez dodatkov.Sample with addition of 0,3 wt. % of the mixture MIXTURE 5 retained the color of fresh meat by 13% compared to the sample with the addition of 0.1 wt. % of the mixture MIXTURE 4, by 13% compared to the sample with the addition of 0.2 wt. % of the mixture MIXTURE 3, by 13% compared to the sample with the addition of 0.2% by weight. % of the mixture MIXTURE 2 by 29% compared to the samples with the addition of 0.3 wt. % of the mixture MIXTURE 1, 1.5 times compared to the sample with the addition of 0.2 wt. % acerola extract and 5 times compared to the sample without additives.

Hkrati so se vzorci z dodatkom mešanic MEŠANICA 1, MEŠANICA 2, MEŠANICA 3, MEŠANICA 4 in MEŠANICA 5 glede na merjenje oksidativnih sprememb (TBA število, ki meri koncentracijo malonaldehida kot sekundarnega produkta oksidacije) in skupnega števila mikroorganizmov pokazali kot oksidativno in mikrobiološko stabilnejši v primerjavi z vzorcem brez dodatkov.At the same time, with the addition of Mixtures 1, Mixture 2, Mixture 3, Mixture 3, Mixture 4 and Mixture 5, samples were found to be more oxidatively and microbiologically more stable with respect to the measurement of oxidative changes (TBA number that measures the concentration of malonaldehyde as a secondary oxidation product) and the total number of microorganisms. compared to the sample without additives.

Antimikrobiološko zaščito mesa in mesnih izdelkov smo še izboljšali z dodatkom ekstrakta zelenega čaja in/ali ekstrakta olivnih listov in/ali ekstrakta citrusov in/ali ekstrakta hmelja in/ali ekstrakta korenja k mešanicam MEŠANICA 1, MEŠANICA 2, MEŠANICA 3, MEŠANICA 4 ali MEŠANICA 5.The antimicrobial protection of meat and meat products was further enhanced by the addition of green tea extract and / or olive leaf extract and / or citrus extract and / or hops extract and / or carrot extract to mixtures MIXTURE 1, MIXTURE 2, MIXTURE 3, MIXTURE 4 5.

Navedeni primeri se nanašajo tudi na ekstrakte ostalih rastlin družine ustnatic kot so žajbelj, melisa, origano, šetraj, meta, timijan in drugi.These examples also apply to extracts from other plants of the lipid family such as sage, lemon balm, oregano, walnut, mint, thyme and others.

-10Vir naravnih rdečih barvil je lahko kateri koli rastlinski material, ki vsebuje rdeče barvilo, kot: grozdje, korenje, redkvica, rdeče zelje, goji, acai, rdeča pesa itd.-10The source of natural red dyes can be any plant material that contains red dye, such as: grapes, carrots, radish, red cabbage, goji, acai, red beet, etc.

Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po izumu je izvedena z dodatkom sinergistične mešanice aktivnih učinkovin na osnovi ekstrakta rastlin družine ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil, prednostno ekstrakta rdeče pese ter opcijsko za izboljšanje antimikrobiološke učinkovitosti z dodatkom ekstrakta zelenega čaja in/ali ekstrakta olivnih listov in/ali ekstrakta citrusov in/ali ekstrakta hmelja in/ali ekstrakta korenja. Naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, je opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenega čaja in/ali EDTA. Stabilizirana mešanica barvil se lahko uporabi tudi samostojno za barvanje mesa kot nadomestek karminske kisline in karmina.The color, dyeing and antioxidant and antimicrobial protection of the meat and meat products of the invention is accomplished by the addition of a synergistic active ingredient mixture based on the plant extract of the lipid family and / or acerola extract and / or natural red dyes, preferably red beet extract and optionally antimicrobial enhancement efficacy by the addition of green tea extract and / or olive leaf extract and / or citrus extract and / or hops extract and / or carrot extract. Natural red colorant, preferably red beet extract, is optionally pre-added with pepper oleoresin and / or carrot extract and / or turmeric extract and / or safflower extract, after which the colorant mixture is stabilized by the addition of CITREM citrus / rosemary / emulsifier or CITREM emulsifier and / or ascorbic acid and / or acerola extract and / or pomegranate and / or tocopherol extracts (mixed or single isomers) and / or lemon and / or green tea extract and / or EDTA. A stabilized dye mixture can also be used independently to dye meat as a substitute for carminic acid and carmine.

Za izboljšanje porazdelitve po mesu ali mesnem izdelku je sinergistični mešanici aktivnih učinkovin dodana sol, maltodekstrin, dekstroza ali drugi v prehrani dovoljen nosilec in/ali v prehrani dovoljen emulgator in/ali v prehrani dovoljeno sredstvo proti strjevanju. Sinergistična mešanica aktivnih učinkovin vsebuje 0-50 % kamozolne kisline in/ali 0-50 % rožmarinske kisline in/ali 0-30 % askorbinske kisline in/ali 0-20 % antocianov in/ali 0-5 % betaninov.To improve the distribution by meat or meat product, a salt, maltodextrin, dextrose or other diet-approved carrier and / or diet-approved emulsifier and / or diet-approved anti-caking agent is added to the synergistic mixture of active substances. The synergistic mixture of the active substances contains 0-50% camosolic acid and / or 0-50% rosemary acid and / or 0-30% ascorbic acid and / or 0-20% anthocyanins and / or 0-5% betanins.

Mesu in mesnim izdelkom se doda mešanica v takšnem doziranju, daje koncentracija aktivnih učinkovin (kamozolne kisline in/ali rožmarinske kisline in/ali askorbinske kisline in/ali antocianov in/ali betanina) v mesu ali mesnem izdelku vsaj 1 ppm. Zaščiteno meso in mesni izdelki ohranijo svežo, rožnato barvo, teksturo, okus in svež vonj.To the meat and meat products, a mixture is added at such a dosage that the concentration of the active substances (camosolic acid and / or rosmarinic acid and / or ascorbic acid and / or anthocyanins and / or betanin) in the meat or meat product is at least 1 ppm. Protected meat and meat products retain a fresh, pink color, texture, taste and a fresh aroma.

Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov se lahko uporabi v kombinaciji z vsemi možnimi pakiranji, kot so: normalno pakiranje, pakiranje v dvojno folijo (vacuum skin packaging), aktivno pakiranje, aktivno pakiranje z dodatki za uravnavanje kisline, pakiranje v folijo z dodatkom zaščitnih novi, pakiranje z vsemi oblikami modificirane atmosfere, pakiranje v kontrolirano atmosfero in drugi.Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products can be used in combination with all possible packaging such as: normal packaging, vacuum skin packaging, active packaging, active packaging with acid regulation additives, packaging in foil with the addition of protective new, packaging with all forms of modified atmosphere, packaging in controlled atmosphere and others.

Claims (8)

1. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov, značilna po tem, daje mesu in mesnim izdelkom dodana sinergistična mešanica aktivnih učinkovin na osnovi ekstrakta rastlin družine ustnatic in/ali ekstrakta acerole in/ali naravnih rdečih barvil, prednostno ekstrakta rdeče pese.1. Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products, characterized in that a synergistic mixture of active ingredients is added to the meat and meat products based on the extract of the plants of the oral family and / or extract of acerola and / or natural red dyes, preferably extract red beets. 2. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, značilna po tem, daje naravnemu rdečemu barvilu, prednostno ekstraktu rdeče pese, opcijsko predhodno dodan oleorezin paprike in/ali ekstrakt korenčka in/ali ekstrakt kurkume in/ali ekstrakt žafranike, nakar je mešanica barvil stabilizirana z dodatkom ekstrakta rožmarina in/ali emulgatorja CITREM in/ali citronske kisline in/ali askorbinske kisline in/ali ekstrakta acerole in/ali ekstrakta šipka in/ali tokoferolov (mešanih ali posameznih izomer) in/ali ekstrakta limone in/ali ekstrakta zelenega čaja in/ali EDTA2. Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that the natural red colorant, preferably red beet extract, optionally pre-added pepper oleoresin and / or carrot extract and / or turmeric extract and / or safflower extract after which the coloring mixture is stabilized by the addition of rosemary extract and / or emulsifier CITREM and / or citric acid and / or ascorbic acid and / or acerola extract and / or extract of rosehip and / or tocopherols (mixed or individual isomers) and / or lemon extract and / or green tea extract and / or EDTA 3. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, značilna po tem, daje navedeni mešanici opcijsko za izboljšanje antimikrobiološke učinkovitosti dodan ekstrakt zelenega čaja in/ali ekstrakt olivnih listov in/ali ekstrakt citrusov in/ali ekstrakt hmelja in/ali ekstrakt korenja.3. Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that said green tea extract and / or olive leaf extract and / or citrus extract and / or extract are added to said mixture optionally to improve antimicrobial efficiency. hops and / or carrot extract. 4. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, značilna po tem, da se za izboljšanje porazdelitve po mesu ali mesnem izdelku sinergistični mešanici aktivnih učinkovin doda sol, maltodekstrin, dekstroza ali drugi v prehrani dovoljen nosilec in/ali v prehrani dovoljen emulgator in/ali v prehrani dovoljeno sredstvo proti strjevanju.4. Color protection, dyeing, and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that salt, maltodextrin, dextrose or other diet-approved carrier are added to improve the distribution of the meat or meat product to the synergistic active ingredient mixture; / or a diet-approved emulsifier and / or a diet-approved anti-caking agent. 5. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, označena s tem, da sinergistična mešanica aktivnih učinkovin vsebuje 0-50 % kamozolne kisline in/ali 0-50 % rožmarinske kisline, 0-30 % askorbinske kisline, 0-20 % antocianov in/ali 0-5 % betaninov.5. Dye protection, dyeing, and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that the synergistic mixture of active ingredients contains 0-50% camosolic acid and / or 0-50% rosemary acid, 0-30% ascorbic acid acids, 0-20% anthocyanins and / or 0-5% betanins. • -f• -f -126. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, značilna po tem, da se mesu in mesnim izdelkom doda mešanica v takšnem doziranju, da je skupna koncentracija aktivnih učinkovin v mesu ali mesnem izdelku vsaj 1 ppm.-126. Dye protection, dyeing, and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that the mixture is added to the meat and meat products in such a dosage that the total active ingredient concentration in the meat or meat product is at least 1 ppm. 7. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, značilna po tem, da zaščiteno meso in mesni izdelki ohranijo svežo, rožnato barvo, teksturo, okus in svež vonj.7. Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that the protected meat and meat products retain a fresh, pink color, texture, taste and a fresh odor. 8. Barvanje mesa in mesnih izdelkov, značilno po tem, da je kot barvilo uporabljena stabilizirana mešanica barvil kot nadomestek karminske kisline in karmina.8. Dyeing of meat and meat products, characterized in that a stabilized dye mixture is used as a dye as a substitute for carminic acid and carmine. 9. Zaščita barve, barvanje ter antioksidativna in antimikrobiološka zaščita mesa in mesnih izdelkov po zahtevku 1, značilna po tem, da se lahko uporabi v kombinaciji z vsemi možnimi pakiranji, kot so: normalno pakiranje, pakiranje v dvojno folijo (vacuum skin packaging), aktivno pakiranje, aktivno pakiranje z dodatki za uravnavanje kisline, pakiranje v folijo z dodatkom zaščitnih novi, pakiranje z vsemi oblikami modificirane atmosfere, pakiranje v kontrolirano atmosfero in drugi.9. Color protection, dyeing and antioxidant and antimicrobial protection of meat and meat products according to claim 1, characterized in that it can be used in combination with all possible packages, such as: normal packaging, double skin packaging (vacuum skin packaging), active packaging, active packaging with acid regulation additives, packaging in foil with the addition of protective new ones, packaging with all forms of modified atmosphere, controlled atmosphere packaging and others.
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