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SG11202111982UA - Synthetic self-replicating rna vectors encoding crispr proteins and uses thereof - Google Patents

Synthetic self-replicating rna vectors encoding crispr proteins and uses thereof

Info

Publication number
SG11202111982UA
SG11202111982UA SG11202111982UA SG11202111982UA SG11202111982UA SG 11202111982U A SG11202111982U A SG 11202111982UA SG 11202111982U A SG11202111982U A SG 11202111982UA SG 11202111982U A SG11202111982U A SG 11202111982UA SG 11202111982U A SG11202111982U A SG 11202111982UA
Authority
SG
Singapore
Prior art keywords
vectors encoding
replicating rna
rna vectors
encoding crispr
crispr proteins
Prior art date
Application number
SG11202111982UA
Inventor
Naohisa Yoshioka
Original Assignee
Emd Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emd Millipore Corp filed Critical Emd Millipore Corp
Publication of SG11202111982UA publication Critical patent/SG11202111982UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11202111982UA 2019-05-13 2020-05-13 Synthetic self-replicating rna vectors encoding crispr proteins and uses thereof SG11202111982UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962847032P 2019-05-13 2019-05-13
PCT/US2020/032688 WO2020232132A1 (en) 2019-05-13 2020-05-13 Synthetic self-replicating rna vectors encoding crispr proteins and uses thereof

Publications (1)

Publication Number Publication Date
SG11202111982UA true SG11202111982UA (en) 2021-11-29

Family

ID=70919231

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202111982UA SG11202111982UA (en) 2019-05-13 2020-05-13 Synthetic self-replicating rna vectors encoding crispr proteins and uses thereof

Country Status (6)

Country Link
US (1) US20220186235A1 (en)
EP (1) EP3969574A1 (en)
JP (2) JP2022533589A (en)
CN (2) CN119709815A (en)
SG (1) SG11202111982UA (en)
WO (1) WO2020232132A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112852841A (en) * 2021-02-03 2021-05-28 郑州大学 Cis replicon RNA construct for efficiently expressing target protein
CN114657181B (en) * 2022-04-01 2023-08-25 安徽大学 A sgRNA targeting H1.4 and H1.4 gene editing method
WO2023216037A1 (en) * 2022-05-07 2023-11-16 上海鲸奇生物科技有限公司 Development of dna-targeting gene editing tool
GB202400648D0 (en) * 2024-01-17 2024-02-28 Uea Enterprises Ltd Protein replacement therapy
CN119162152B (en) * 2024-11-18 2025-02-25 南京农业大学三亚研究院 A highly accurate base editor that expands editing range and improves editing efficiency

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS501B1 (en) 1970-05-19 1975-01-06
EP1456374A4 (en) 2001-11-26 2005-08-17 Advanced Cell Tech Inc METHODS FOR THE PRODUCTION AND USE OF REPROGRAMME HUMAN SOMATIC CELL CORES AND AUTOLOGOUS AND ISOGENIC HUMAN STEM CELLS
US20060198854A1 (en) * 2004-12-28 2006-09-07 Peter Pushko Vector platforms derived from the alphavirus vaccines
AU2013343864B2 (en) * 2012-11-09 2019-04-04 BioNTech SE Method for cellular RNA expression
WO2017053851A1 (en) * 2015-09-23 2017-03-30 Massachusetts Institute Of Technology Compositions and methods for modified dendrimer nanoparticle vaccine delivery
US20200399609A1 (en) * 2016-05-10 2020-12-24 Ingeneron, Inc. Self replicating rna for inducing somatic differentiation of unmodified adult stem cells
US11845942B2 (en) * 2017-06-09 2023-12-19 Vilmorin & Cie Compositions and methods for genome editing
CN111183226A (en) * 2017-07-11 2020-05-19 西格马-奥尔德里奇有限责任公司 Use of nucleosome interacting protein domains to enhance targeted genomic modifications
SG11201912024RA (en) * 2017-07-31 2020-02-27 Sigma Aldrich Co Llc Synthetic guide rna for crispr/cas activator systems
US11932858B2 (en) * 2017-12-14 2024-03-19 Donald Danforth Plant Science Center Homologous recombination via transcriptional activation
US20190202856A1 (en) * 2017-12-29 2019-07-04 Sigma-Aldrich Co. Llc Engineered crispr proteins for covalent tagging nucleic acids

Also Published As

Publication number Publication date
CN119709815A (en) 2025-03-28
EP3969574A1 (en) 2022-03-23
US20220186235A1 (en) 2022-06-16
WO2020232132A1 (en) 2020-11-19
CN114174500A (en) 2022-03-11
JP2022533589A (en) 2022-07-25
JP2024032973A (en) 2024-03-12

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