[go: up one dir, main page]

SG11202009372UA - Systems and methods for quantifying and modifying protein viscosity - Google Patents

Systems and methods for quantifying and modifying protein viscosity

Info

Publication number
SG11202009372UA
SG11202009372UA SG11202009372UA SG11202009372UA SG11202009372UA SG 11202009372U A SG11202009372U A SG 11202009372UA SG 11202009372U A SG11202009372U A SG 11202009372UA SG 11202009372U A SG11202009372U A SG 11202009372UA SG 11202009372U A SG11202009372U A SG 11202009372UA
Authority
SG
Singapore
Prior art keywords
quantifying
systems
methods
modifying protein
protein viscosity
Prior art date
Application number
SG11202009372UA
Inventor
Xiaobin Xu
Aming Zhang
Yuan Cao
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of SG11202009372UA publication Critical patent/SG11202009372UA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/36Extraction; Separation; Purification by a combination of two or more processes of different types
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4005Concentrating samples by transferring a selected component through a membrane
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4005Concentrating samples by transferring a selected component through a membrane
    • G01N2001/4016Concentrating samples by transferring a selected component through a membrane being a selective membrane, e.g. dialysis or osmosis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/15Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00Chemical aspects of mass spectrometric analysis of biological material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
SG11202009372UA 2018-05-10 2019-05-09 Systems and methods for quantifying and modifying protein viscosity SG11202009372UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862669440P 2018-05-10 2018-05-10
PCT/US2019/031438 WO2019217626A1 (en) 2018-05-10 2019-05-09 Systems and methods for quantifying and modifying protein viscosity

Publications (1)

Publication Number Publication Date
SG11202009372UA true SG11202009372UA (en) 2020-10-29

Family

ID=66794086

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009372UA SG11202009372UA (en) 2018-05-10 2019-05-09 Systems and methods for quantifying and modifying protein viscosity

Country Status (14)

Country Link
US (1) US20190345196A1 (en)
EP (1) EP3791189A1 (en)
JP (1) JP2021523349A (en)
KR (1) KR20210007958A (en)
CN (1) CN112136049A (en)
AR (1) AR115383A1 (en)
AU (1) AU2019266275A1 (en)
BR (1) BR112020021140A2 (en)
CA (1) CA3095190A1 (en)
EA (1) EA202092694A1 (en)
MX (1) MX2020012016A (en)
SG (1) SG11202009372UA (en)
TW (1) TW202016125A (en)
WO (1) WO2019217626A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022187323A1 (en) * 2021-03-03 2022-09-09 Regeneron Pharmaceuticals, Inc. Systems and methods for quantifying and modifying protein viscosity
WO2024208261A1 (en) * 2023-04-04 2024-10-10 南京金斯瑞生物科技有限公司 Viscosity prediction method for antibody drug development

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7087411B2 (en) 1999-06-08 2006-08-08 Regeneron Pharmaceuticals, Inc. Fusion protein capable of binding VEGF
KR100659477B1 (en) * 1999-06-08 2006-12-20 리제네론 파라마큐티칼스 인코포레이티드 Modified chimeric polypeptides with improved pharmacokinetic properties
JP4340062B2 (en) * 2000-10-12 2009-10-07 ジェネンテック・インコーポレーテッド Concentrated protein preparation with reduced viscosity
TW574597B (en) 2002-03-08 2004-02-01 Asml Netherlands Bv Mask for use in lithography, method of making a mask, lithographic apparatus, and device manufacturing method
ES2377579T3 (en) 2006-06-02 2012-03-29 Regeneron Pharmaceuticals, Inc. High-affinity antibodies against the human IL-6 receptor
US7608693B2 (en) 2006-10-02 2009-10-27 Regeneron Pharmaceuticals, Inc. High affinity human antibodies to human IL-4 receptor
JP5588865B2 (en) 2007-07-31 2014-09-10 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Human antibodies against human CD20 and methods of use thereof
US8309088B2 (en) 2007-08-10 2012-11-13 Regeneron Pharmaceuticals, Inc. Method of treating osteoarthritis with an antibody to NGF
JO3672B1 (en) 2008-12-15 2020-08-27 Regeneron Pharma High Affinity Human Antibodies to PCSK9
MX342623B (en) 2009-06-26 2016-10-06 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format.
JO3417B1 (en) 2010-01-08 2019-10-20 Regeneron Pharma Stabilized formulations containing anti-interleukin-6 receptor (il-6r) antibodies
JO3340B1 (en) 2010-05-26 2019-03-13 Regeneron Pharma Antibodies to human gdf8
JOP20190250A1 (en) 2010-07-14 2017-06-16 Regeneron Pharma Stable formulas containing anti-NGF antibodies
AR083044A1 (en) 2010-09-27 2013-01-30 Regeneron Pharma ANTI-CD48 ANTIBODIES AND USES OF THE SAME
NZ609557A (en) 2010-10-06 2014-12-24 Regeneron Pharma Stabilized formulations containing anti-interleukin-4 receptor (il-4r) antibodies
JO3756B1 (en) 2010-11-23 2021-01-31 Regeneron Pharma Human antibodies to the glucagon receptor
AR087329A1 (en) 2011-06-17 2014-03-19 Regeneron Pharma HUMAN ANTIBODIES AGAINST PROTEIN 3 OF HUMAN ANGIOPOIETIN TYPE
AU2012339722B2 (en) 2011-11-14 2017-09-14 Regeneron Pharmaceuticals, Inc. Compositions and methods for increasing muscle mass and muscle strength by specifically antagonizing GDF8 and/or Activin A
JO3533B1 (en) 2012-01-23 2020-07-05 Regeneron Pharma Stabilized formulations containing anti-ang2 antibodies
JO3820B1 (en) 2012-05-03 2021-01-31 Regeneron Pharma Human antibodies to FEL D1 and methods for their use
TW201843172A (en) 2012-06-25 2018-12-16 美商再生元醫藥公司 anti-EGFR antibody and use thereof
CN104540852B (en) 2012-08-13 2018-10-02 瑞泽恩制药公司 Anti- PCSK9 antibody with pH- dependence binding characteristics
JOP20200236A1 (en) 2012-09-21 2017-06-16 Regeneron Pharma Anti-cd3 antibodies, bispecific antigen-binding molecules that bind cd3 and cd20, and uses thereof
JO3405B1 (en) 2013-01-09 2019-10-20 Regeneron Pharma ANTI-PDGFR-beta ANTIBODIES AND USES THEREOF
JO3532B1 (en) 2013-03-13 2020-07-05 Regeneron Pharma Anti-il-33 antibodies and uses thereof
TWI659968B (en) 2013-03-14 2019-05-21 再生元醫藥公司 Human antibodies to respiratory syncytial virus f protein and methods of use thereof
CN105007929B (en) 2013-03-15 2019-05-10 瑞泽恩制药公司 IL-33 antagonist and its purposes
TWI641620B (en) 2013-08-21 2018-11-21 再生元醫藥公司 Anti-prlr antibodies and uses thereof
TWI680138B (en) 2014-01-23 2019-12-21 美商再生元醫藥公司 Human antibodies to pd-l1
TWI681969B (en) 2014-01-23 2020-01-11 美商再生元醫藥公司 Human antibodies to pd-1
US9475875B2 (en) 2014-03-11 2016-10-25 Regeneron Pharmaceuticals, Inc. Anti-EGFRvIII antibodies and uses thereof
TWI754319B (en) 2014-03-19 2022-02-01 美商再生元醫藥公司 Methods and antibody compositions for tumor treatment
CA3225091A1 (en) 2014-05-05 2015-11-12 Regeneron Pharmaceuticals, Inc. Humanized c5 and c3 animals
JO3701B1 (en) 2014-05-23 2021-01-31 Regeneron Pharma Human antibodies to middle east respiratory syndrome – coronavirus spike protein
SG11201701711VA (en) 2014-09-16 2017-04-27 Regeneron Pharma Anti-glucagon antibodies and uses thereof
US10894818B2 (en) * 2014-10-03 2021-01-19 Massachusetts Institute Of Technology Antibodies that bind Ebola glycoprotein and uses thereof
JP2017536833A (en) * 2014-12-01 2017-12-14 ザ スクリプス リサーチ インスティテュート Methods and compositions related to functional polypeptides incorporated into heterologous protein scaffolds
TWI710573B (en) 2015-01-26 2020-11-21 美商再生元醫藥公司 Human antibodies to ebola virus glycoprotein

Also Published As

Publication number Publication date
KR20210007958A (en) 2021-01-20
EP3791189A1 (en) 2021-03-17
AR115383A1 (en) 2021-01-13
CA3095190A1 (en) 2019-11-14
AU2019266275A1 (en) 2020-11-05
BR112020021140A2 (en) 2021-02-17
MX2020012016A (en) 2021-01-29
JP2021523349A (en) 2021-09-02
CN112136049A (en) 2020-12-25
US20190345196A1 (en) 2019-11-14
WO2019217626A1 (en) 2019-11-14
EA202092694A1 (en) 2021-03-12
TW202016125A (en) 2020-05-01

Similar Documents

Publication Publication Date Title
SG11202100588SA (en) Systems and methods for metabolome analysis
SG11201811762RA (en) Systems and methods for path determination
ZA202003999B (en) System and methods
EP3669306A4 (en) System and method for identifying vaping and bullying
SG11202008774YA (en) Electrohydrodynamic bioprinter system and method
IL272228B2 (en) System and Method for Non-Invasively Determining Egg Properties
IL290612A (en) Systems and methods for predicting proteins
EP3805726A4 (en) Inspection system and inspection method
GB201804807D0 (en) Interaactive systems and methods
GB201807293D0 (en) Biosensor method and system
EP3829812A4 (en) Minimum quantity lubrication system and method
GB2572956B (en) Calibration system and method
GB2572677B (en) System and method
IL291284A (en) Systems and methods for protein expression
EP3874580A4 (en) System and method for determining q factor
IL276102A (en) System and method for characterizing protein dimerization
EP3815028A4 (en) Method and system for risk determination
IL257059B (en) Multi-beamforming system and method
SG11202009372UA (en) Systems and methods for quantifying and modifying protein viscosity
GB201816668D0 (en) System and method
GB201809582D0 (en) System and method
GB201801922D0 (en) Surrogate neck and method
GB201803940D0 (en) Methods and systems for analysis
IL257582B (en) Conveyors interfacing system and method
GB2576029B (en) Illumination system and method