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SG11202003863VA - Casz compositions and methods of use - Google Patents

Casz compositions and methods of use

Info

Publication number
SG11202003863VA
SG11202003863VA SG11202003863VA SG11202003863VA SG11202003863VA SG 11202003863V A SG11202003863V A SG 11202003863VA SG 11202003863V A SG11202003863V A SG 11202003863VA SG 11202003863V A SG11202003863V A SG 11202003863VA SG 11202003863V A SG11202003863V A SG 11202003863VA
Authority
SG
Singapore
Prior art keywords
casz
compositions
methods
casz compositions
Prior art date
Application number
SG11202003863VA
Inventor
Jennifer A Doudna
David Burstein
Janice S Chen
Lucas B Harrington
David Paez-Espino
Jillian F Banfield
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of SG11202003863VA publication Critical patent/SG11202003863VA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202003863VA 2017-11-01 2018-10-31 Casz compositions and methods of use SG11202003863VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762580395P 2017-11-01 2017-11-01
PCT/US2018/058545 WO2019089820A1 (en) 2017-11-01 2018-10-31 Casz compositions and methods of use

Publications (1)

Publication Number Publication Date
SG11202003863VA true SG11202003863VA (en) 2020-05-28

Family

ID=66332730

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202003863VA SG11202003863VA (en) 2017-11-01 2018-10-31 Casz compositions and methods of use

Country Status (14)

Country Link
US (7) US20200087640A1 (en)
EP (1) EP3704239A4 (en)
JP (2) JP2021503278A (en)
KR (1) KR20200091858A (en)
CN (2) CN111886336A (en)
AU (1) AU2018358051B2 (en)
BR (1) BR112020008654A2 (en)
CA (1) CA3080493A1 (en)
GB (1) GB2582482B (en)
IL (1) IL274276A (en)
MX (1) MX2020004578A (en)
RU (1) RU2020117711A (en)
SG (1) SG11202003863VA (en)
WO (1) WO2019089820A1 (en)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010101628A2 (en) 2009-03-02 2010-09-10 Massachusetts Institute Of Technology Methods and products for in vivo enzyme profiling
AU2012229103A1 (en) 2011-03-15 2013-10-31 Massachusetts Institute Of Technology Multiplexed detection with isotope-coded reporters
ES2828985T3 (en) 2013-06-07 2021-05-28 Massachusetts Inst Technology Affinity-based detection of synthetic ligand-encoded biomarkers
CA2963693A1 (en) 2014-10-10 2016-04-14 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2017177115A1 (en) 2016-04-08 2017-10-12 Massachusetts Institute Of Technology Methods to specifically profile protease activity at lymph nodes
CA3022928A1 (en) 2016-05-05 2017-11-09 Massachusetts Institute Of Technology Methods and uses for remotely triggered protease activity measurements
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
EA201990860A1 (en) 2016-09-30 2019-10-31 RNA-GUIDED MODIFICATING NUCLEIC ACIDS ENZYMES AND METHODS OF APPLICATION
EA201990861A1 (en) 2016-09-30 2019-09-30 Те Риджентс Оф Те Юниверсити Оф Калифорния RNA-GUIDED MODIFICATING NUCLEIC ACIDS ENZYMES AND METHODS OF APPLICATION
EP4095263A1 (en) 2017-01-06 2022-11-30 Editas Medicine, Inc. Methods of assessing nuclease cleavage
CA3059358A1 (en) 2017-04-07 2018-10-11 Massachusetts Institute Of Technology Methods to spatially profile protease activity in tissue and sections
US11499151B2 (en) 2017-04-28 2022-11-15 Editas Medicine, Inc. Methods and systems for analyzing guide RNA molecules
CN110997908A (en) 2017-06-09 2020-04-10 爱迪塔斯医药公司 Engineered CAS9 nucleases
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
SG11202003863VA (en) 2017-11-01 2020-05-28 Univ California Casz compositions and methods of use
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
WO2019089808A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Class 2 crispr/cas compositions and methods of use
KR20200124702A (en) 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 The novel CAS9 ortholog
CN110551800B (en) * 2018-06-03 2022-08-19 上海吐露港生物科技有限公司 Application of high-temperature-resistant Cas protein, and detection method and kit of target nucleic acid molecule
EP3830301B1 (en) 2018-08-01 2024-05-22 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
EP3844303A4 (en) * 2018-08-27 2022-06-01 The Regents of The University of California REPORTER NUCLEIC ACIDS FOR TYPE V CRISPR-MEDIATED DETECTION
WO2020068920A2 (en) 2018-09-25 2020-04-02 Massachusetts Institute Of Technology Lung protease nanosensors and uses thereof
CA3117228A1 (en) * 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US11835522B2 (en) 2019-01-17 2023-12-05 Massachusetts Institute Of Technology Sensors for detecting and imaging of cancer metastasis
AU2020231380A1 (en) 2019-03-07 2021-09-23 The Regents Of The University Of California CRISPR-Cas effector polypeptides and methods of use thereof
JP2022530981A (en) * 2019-05-01 2022-07-05 マンモス バイオサイエンシズ インコーポレイテッド Programmable nuclease and usage
KR102455623B1 (en) 2019-10-29 2022-10-18 주식회사 진코어 An engineered guide RNA for the optimized CRISPR/Cas12f1 system and use thereof
WO2021167687A1 (en) 2020-02-18 2021-08-26 Massachusetts Institute Of Technology Multiplexed in vivo disease sensing with nucleic acid-barcoded reporters
CN111257297B (en) * 2020-03-31 2023-06-16 海南大学 RNA detection method based on Cas14a enzyme
CN115427566A (en) 2020-04-08 2022-12-02 阿斯利康(瑞典)有限公司 Compositions and methods for improved site-specific modification
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
AU2021357377A1 (en) 2020-10-08 2023-05-25 Genkore Inc. Engineered guide RNA for optimized CRISPR/Cas12f1 (Cas14a1) system and use thereof
US20230416784A1 (en) 2020-10-08 2023-12-28 Genkore Inc. Engineered guide rna for optimized crispr/cas12f1 (cas14a1) system and use thereof
KR102758158B1 (en) * 2021-04-21 2025-01-23 주식회사 진코어 An engineered guide RNA for the optimized CRISPR/Cas12f1 system and use thereof
EP4227408A1 (en) 2020-10-08 2023-08-16 Genkore Inc Engineered guide rna comprising u-rich tail for increasing efficiency of crispr/cas12f1 system, and use thereof
KR102638799B1 (en) * 2020-10-08 2024-02-22 주식회사 진코어 An engineered guide RNA for the optimized CRISPR/Cas12f1(Cas14a1) system and use thereof
CN116568803A (en) 2020-10-08 2023-08-08 基恩科雷有限责任公司 Engineered guide RNAs comprising U-rich tails for increasing efficiency of CRISPR/Cas12f1 systems and uses thereof
CN112176035A (en) * 2020-10-14 2021-01-05 杭州优思达生物技术有限公司 Novel CRISPR nucleic acid detection method and application
WO2022092317A1 (en) * 2020-10-30 2022-05-05 国立大学法人東京大学 ENGINEERED Cas12f PROTEIN
EP4337701A1 (en) 2021-05-10 2024-03-20 Mammoth Biosciences, Inc. Effector proteins and methods of use
WO2022241032A1 (en) * 2021-05-12 2022-11-17 Mammoth Biosciences, Inc. Enhanced guide nucleic acids and methods of use
EP4342986A1 (en) 2021-05-14 2024-03-27 Genkore Inc Composition and method for treatment of lca10 using rna-guided nuclease
JP2024520528A (en) 2021-06-01 2024-05-24 アーバー バイオテクノロジーズ， インコーポレイテッド Gene editing systems containing CRISPR nucleases and uses thereof
EP4368713A1 (en) 2021-07-05 2024-05-15 Genkore Inc. Cleavage-inactive cas12f1, cleavage-inactive cas12f1-based fusion protein, crispr gene-editing system comprising same, and preparation method and use thereof
US12139731B2 (en) 2021-07-08 2024-11-12 National Institutes Of Health (Nih), U.S. Dept. Of Health And Human Services (Dhhs) U.S. Government CRISPR-based programmable RNA editing
US11814689B2 (en) 2021-07-21 2023-11-14 Montana State University Nucleic acid detection using type III CRISPR complex
WO2023086938A2 (en) * 2021-11-12 2023-05-19 Arbor Biotechnologies, Inc. Type v nucleases
GB2631171A (en) 2021-12-03 2024-12-25 Broad Inst Inc Compositions and methods for efficient in vivo delivery
AU2023229364A1 (en) * 2022-03-01 2024-09-19 Epicrispr Biotechnologies, Inc. Engineered nucleases, compositions, and methods of use thereof
KR20230134098A (en) 2022-03-10 2023-09-20 주식회사 진코어 Gene editing system for treating duchenne muscular dystrophy and method of treating the disease using the same
CN114561456A (en) * 2022-03-23 2022-05-31 北京盛因生物科技有限公司 A kind of isothermal nucleic acid detection enzyme composition, kit and use and detection method thereof
EP4502153A1 (en) 2022-03-30 2025-02-05 GenKOre Inc. Gene editing system for treating usher syndrome
WO2023201275A1 (en) * 2022-04-13 2023-10-19 The Board Of Trustees Of The Leland Stanford Junior University Enzyme kinetics analyses of crispr endonucleases
EP4514953A1 (en) * 2022-04-25 2025-03-05 Huidagene Therapeutics (Singapore) Pte. Ltd. Novel crispr-cas12f systems and uses thereof
TW202440938A (en) 2023-02-15 2024-10-16 美商喬木生物技術公司 Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript
WO2025019273A1 (en) * 2023-07-14 2025-01-23 Epicrispr Biotechnologies, Inc. Engineered nucleases and guide rna spacer sequences therefor, and compositions, and methods of use thereof
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
CN117512071B (en) * 2023-09-15 2024-06-04 湖北大学 High temperature-resistant TnpB protein and application thereof in nucleic acid detection
CN119162153B (en) * 2024-11-22 2025-02-07 镁孚泰生物科技(上海)有限公司 Cas12f nuclease mutant, its application and kit

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004521606A (en) 2000-05-24 2004-07-22 サード・ウェーブ・テクノロジーズ・インク RNA detection method
US6773885B1 (en) 2000-09-29 2004-08-10 Integrated Dna Technologies, Inc. Compositions and methods for visual ribonuclease detection assays
EP1580273A1 (en) * 2004-03-26 2005-09-28 Friedrich-Alexander-Universität Erlangen-Nürnberg Peptide-based method for monitoring gene expression in a host cell
CN101283089A (en) * 2005-10-07 2008-10-08 加利福尼亚大学董事会 Encoding nucleic acid for modifying cytochrome P450 enzyme and its application method
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US8597886B2 (en) 2010-02-15 2013-12-03 Cascade Biosystems, Inc. Methods and materials for detecting viral or microbial infections
JP2013531983A (en) 2010-06-11 2013-08-15 パソジェニカ，インコーポレイテッド Nucleic acids for multiplex biological detection and methods of use and production thereof
US9730967B2 (en) 2011-02-04 2017-08-15 Katherine Rose Kovarik Method and system for treating cancer cachexia
US8815782B2 (en) 2011-11-11 2014-08-26 Agilent Technologies, Inc. Use of DNAzymes for analysis of an RNA sample
LT2800811T (en) 2012-05-25 2017-09-11 The Regents Of The University Of California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
US20140189896A1 (en) 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
EP3620534B1 (en) * 2013-03-14 2021-10-13 Caribou Biosciences, Inc. Crispr-cas compositions of nucleic acid-targeting nucleic acids
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
EP3375877A1 (en) 2013-11-18 2018-09-19 Crispr Therapeutics AG Crispr-cas system materials and methods
US9994831B2 (en) 2013-12-12 2018-06-12 The Regents Of The University Of California Methods and compositions for modifying a single stranded target nucleic acid
US9850525B2 (en) 2014-01-29 2017-12-26 Agilent Technologies, Inc. CAS9-based isothermal method of detection of specific DNA sequence
JP2017514513A (en) 2014-03-20 2017-06-08 ユニベルシテ ラバル CRISPR-based methods and products for increasing frataxin levels and uses thereof
EP3129488B1 (en) * 2014-04-10 2019-06-12 The Regents of The University of California Methods and compositions for using argonaute to modify a single stranded target nucleic acid
WO2015191693A2 (en) 2014-06-10 2015-12-17 Massachusetts Institute Of Technology Method for gene editing
CN107075546B (en) * 2014-08-19 2021-08-31 哈佛学院董事及会员团体 RNA-guided system for detection and mapping of nucleic acids
EP3889260A1 (en) 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
WO2016106236A1 (en) 2014-12-23 2016-06-30 The Broad Institute Inc. Rna-targeting system
US11180792B2 (en) 2015-01-28 2021-11-23 The Regents Of The University Of California Methods and compositions for labeling a single-stranded target nucleic acid
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
KR102575342B1 (en) 2015-06-18 2023-09-05 더 브로드 인스티튜트, 인코퍼레이티드 CRISPR enzyme mutations that reduce off-target effects
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CA3012607A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzymes and systems
CA3012631A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
US9580727B1 (en) * 2015-08-07 2017-02-28 Caribou Biosciences, Inc. Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides
KR102761827B1 (en) 2015-10-22 2025-02-03 더 브로드 인스티튜트, 인코퍼레이티드 Type VI-B CRISPR enzymes and systems
US11193127B2 (en) * 2016-01-08 2021-12-07 University Of Georgia Research Foundation, Inc. Methods for cleaving DNA and RNA molecules
US11441146B2 (en) 2016-01-11 2022-09-13 Christiana Care Health Services, Inc. Compositions and methods for improving homogeneity of DNA generated using a CRISPR/Cas9 cleavage system
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
EP3420083A4 (en) 2016-02-23 2019-08-28 Arc Bio, LLC Methods and compositions for target detection
WO2017176529A1 (en) 2016-04-06 2017-10-12 Temple Univesity-Of The Commonwealth System Of Higher Education Compositions for eradicating flavivirus infections in subjects
WO2017205668A1 (en) 2016-05-25 2017-11-30 Arizona Board Of Regents On Behalf Of Arizona State University Portable, low-cost pathogen detection and strain identification platform
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
WO2017219027A1 (en) 2016-06-17 2017-12-21 The Broad Institute Inc. Type vi crispr orthologs and systems
CN109688820B (en) 2016-06-24 2023-01-10 科罗拉多州立大学董事会（法人团体） Method for generating barcoded combinatorial libraries
EP3500967A1 (en) 2016-08-17 2019-06-26 The Broad Institute, Inc. Methods for identifying class 2 crispr-cas systems
EA201990860A1 (en) 2016-09-30 2019-10-31 RNA-GUIDED MODIFICATING NUCLEIC ACIDS ENZYMES AND METHODS OF APPLICATION
EA201990861A1 (en) 2016-09-30 2019-09-30 Те Риджентс Оф Те Юниверсити Оф Калифорния RNA-GUIDED MODIFICATING NUCLEIC ACIDS ENZYMES AND METHODS OF APPLICATION
CN106701830B (en) * 2016-12-07 2020-01-03 湖南人文科技学院 Pig embryo p66 knock-outshcMethod for gene
ES2927463T3 (en) 2016-12-09 2022-11-07 Broad Inst Inc Diagnostics based on the CRISPR effector system
EP3601576A1 (en) 2017-03-24 2020-02-05 CureVac AG Nucleic acids encoding crispr-associated proteins and uses thereof
US11591589B2 (en) 2017-04-21 2023-02-28 The General Hospital Corporation Variants of Cpf1 (Cas12a) with altered PAM specificity
US10316324B2 (en) * 2017-08-09 2019-06-11 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
WO2019089808A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Class 2 crispr/cas compositions and methods of use
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
EP3704254A4 (en) 2017-11-01 2021-09-01 The Regents of The University of California CAS12C COMPOSITIONS AND METHOD OF USE
SG11202003863VA (en) 2017-11-01 2020-05-28 Univ California Casz compositions and methods of use
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
KR20200111180A (en) 2017-12-22 2020-09-28 더 브로드 인스티튜트, 인코퍼레이티드 Multiple diagnostics based on the CRISPR effector system

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