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SG11201900669WA - Engineered imine reductases and methods for the reductive amination of ketone and amine compounds - Google Patents

Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Info

Publication number
SG11201900669WA
SG11201900669WA SG11201900669WA SG11201900669WA SG11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA SG 11201900669W A SG11201900669W A SG 11201900669WA
Authority
SG
Singapore
Prior art keywords
california
redwood city
international
penobscot
drive
Prior art date
Application number
SG11201900669WA
Inventor
Margie Borra-Garske
Oscar Alvizo
Melissa Mayo
Stephan Jenne
Auric Sowell-Kantz
Carmela Molinaro
Original Assignee
Codexis Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis Inc filed Critical Codexis Inc
Publication of SG11201900669WA publication Critical patent/SG11201900669WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 01 March 2018 (01.03.2018) o I iolo 011111 OM REHM IMO 011 (10) International Publication Number WO 2018/038906 Al WIPO I PCT (51) International Patent Classification: CO7H 21/00 (2006.01) C12N 9/06 (2006.01) C07K 14/435 (2006.01) Cl 2N 15/52 (2006.01) C12N 9/00 (2006.01) C12N 15/53 (2006.01) (21) International Application Number: PCT/US2017/045838 (22) International Filing Date: 08 August 2017 (08.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/380,165 26 August 2016 (26.08.2016) US (71) Applicant: CODEXIS, INC. [US/US]; 200 Penobscot Dri- ve, Redwood City, California 94063 (US). (72) Inventors: BORRA-GARSKE, Margie, Tabuga; 200 Penobscot Drive, Redwood City, California 94063 (US). ALVIZO, Oscar; 200 Penobscot Drive, Redwood City, California 94063 (US). MAYO, Melissa, Ann; 200 Penob- scot Drive, Redwood City, California 94063 (US). JENNE, Stephan; 200 Penobscot Drive, Redwood City, California 94063 (US). SOWELL-KANTZ, Auric, Anthony; 200 Penobscot Drive, Redwood City, California 94063 (US). MOLINARO, Carmela; 126 East Lincoln Avenue, Rah- way, New Jersey 07065 (US). TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a)) (74) Agent: MACKNIGHT, Kamrin, T.; Codexis, Inc., 200 Penobscot Drive, Redwood City, California 94063 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 1-1 TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, O GC Cr) O (54) Title: ENGINEERED MINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS 0 (57) : The present application provides engineered polypeptides having imine or oxime reductase activity, polynucleotides N encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engi- C neered polypeptides with a range of ketone amine substrate compounds to prepare secondary and tertiary amine product compounds.
SG11201900669WA 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds SG11201900669WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662380165P 2016-08-26 2016-08-26
PCT/US2017/045838 WO2018038906A1 (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Publications (1)

Publication Number Publication Date
SG11201900669WA true SG11201900669WA (en) 2019-03-28

Family

ID=61245145

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10202111589RA SG10202111589RA (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
SG11201900669WA SG11201900669WA (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10202111589RA SG10202111589RA (en) 2016-08-26 2017-08-08 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Country Status (8)

Country Link
US (3) US11046936B2 (en)
EP (2) EP4223766A3 (en)
JP (2) JP7316662B2 (en)
CN (1) CN109863162B (en)
CA (1) CA3032519A1 (en)
IL (1) IL264686B2 (en)
SG (2) SG10202111589RA (en)
WO (1) WO2018038906A1 (en)

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* Cited by examiner, † Cited by third party
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IL264686B2 (en) * 2016-08-26 2024-07-01 Codexis Inc Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
US12215368B2 (en) 2019-05-01 2025-02-04 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
CN110819601B (en) * 2019-11-04 2021-07-20 江南大学 Reductase, encoding gene, recombinant vector, recombinant cell and application thereof
CN114317472B (en) * 2021-12-31 2022-07-05 长兴制药股份有限公司 High stereoselectivity imine reductase and preparation method and application thereof
CN115838697A (en) * 2022-10-13 2023-03-24 华东理工大学 Imine reductase mutant and application thereof in synthesis of chiral intermediate of erlotinib

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Also Published As

Publication number Publication date
EP3515926B1 (en) 2023-10-25
SG10202111589RA (en) 2021-11-29
CN109863162A (en) 2019-06-07
US11046936B2 (en) 2021-06-29
EP4223766A3 (en) 2023-10-18
US20240254457A1 (en) 2024-08-01
US20210284973A1 (en) 2021-09-16
IL264686B2 (en) 2024-07-01
CA3032519A1 (en) 2018-03-01
US20200208123A1 (en) 2020-07-02
EP3515926A1 (en) 2019-07-31
JP2019526252A (en) 2019-09-19
WO2018038906A1 (en) 2018-03-01
CN109863162B (en) 2023-03-17
EP4223766A2 (en) 2023-08-09
IL264686B1 (en) 2024-03-01
IL264686A (en) 2019-04-30
JP2022065092A (en) 2022-04-26
US11987818B2 (en) 2024-05-21
JP7316662B2 (en) 2023-07-28
EP3515926A4 (en) 2020-04-22

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