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SG11201900442PA - Sorting of t lymphocytes in a microfluidic device - Google Patents

Sorting of t lymphocytes in a microfluidic device

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Publication number
SG11201900442PA
SG11201900442PA SG11201900442PA SG11201900442PA SG11201900442PA SG 11201900442P A SG11201900442P A SG 11201900442PA SG 11201900442P A SG11201900442P A SG 11201900442PA SG 11201900442P A SG11201900442P A SG 11201900442PA SG 11201900442P A SG11201900442P A SG 11201900442PA
Authority
SG
Singapore
Prior art keywords
lymphocytes
suite
emeryville
california
street
Prior art date
Application number
SG11201900442PA
Inventor
Kevin D Loutherback
Yelena Bronevetsky
Peter J Beemiller
Xiaohua Wang
Kevin T Chapman
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11201900442PA publication Critical patent/SG11201900442PA/en

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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
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    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
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    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
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    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

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Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1#111011110111010101111101 1101001111113111011111010111n#011# Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\" WO 2018/018017 Al 25 January 2018 (25.01.2018) WI P0 I P C T (51) International Patent Classification: (72) Inventors; and A61K 35/14 (2015.01) GO1N 1/34 (2006.01) (71) Applicants: LOUTHERBACK, Kevin D. [US/US]; 5858 A61K 35/28 (2015.01) Horton Street, Suite 320, Emeryville, California 94608 (US). BRONEVETSKY, Yelena [US/US]; 5858 Hor- (21) International Application Number: PCT/US2017/043395 ton Street, Suite 320, Emeryville, California 94608 (US). BEEMILLER, Peter J. [US/US]; 5858 Horton Street, (22) International Filing Date: Suite 320, Emeryville, California 94608 (US). WANG, Xi- 21 July 2017 (21.07.2017) aohua [CN/US]; 5858 Horton Street, Suite 320, Emeryville, California 94608 (US). CHAPMAN, Kevin T. [US/US]; (25) Filing Language: English 5858 Horton Street, Suite 320, Emeryville, California 94608 (26) Publication Language: English (US). (30) Priority Data: (74) Agent: HORTON, Kenneth E.; Kirton McConkie, 36 S. 62/365,372 21 July 2016 (21.07.2016) US State Street, Suite 1900, Salt Lake City, Utah 84111 (US). (71) Applicant: BERKELEY LIGHTS, INC. [US/US]; 5858 (81) Designated States (unless otherwise indicated, for every Horton Street, Suite 320, Emeryville, California 94608 kind of national protection available): AE, AG, AL, AM, (US). AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, = (54) Title: SORTING OF T LYMPHOCYTES IN A MICROFLUIDIC DEVICE = 715 ---% 1 = = — = EE = = _ = = = = = - = 1 i 708/710 720 • '' ' . ,, - - - _ 730 ,-, FIG. 7B 702 IN Il (57) : Methods of sorting T lymphocytes in a microfluidic device are provided. The methods can include flowing a fluid 0 of; sample comprising T lymphocytes through a region of a microfluidic device that contains an array of posts. The array of posts can be Il configured to have a critical size (D o ) that separates activated T lymphocytes from nave T lymphocytes. Also provided are microfluidic --... © devices having an array of posts configured to separate activated T lymphocytes from nave T lymphocytes, compositions enriched for Ot T lymphocytes, particularly activated T lymphocytes that are known to be reactive to an antigen of interest, and methods of treating 1-1 c:::: ) subjects suffering from a pathogenic disorder or cancer by administering such compositions. ei C [Continued on next page] WO 2018/018017 Al MIDEDIMOMOIDEIREEMOOMMOHMOIRMERMIS KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3))
SG11201900442PA 2016-07-21 2017-07-21 Sorting of t lymphocytes in a microfluidic device SG11201900442PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662365372P 2016-07-21 2016-07-21
PCT/US2017/043395 WO2018018017A1 (en) 2016-07-21 2017-07-21 Sorting of t lymphocytes in a microfluidic device

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SG11201900442PA true SG11201900442PA (en) 2019-02-27

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US (1) US11666912B2 (en)
EP (1) EP3487510B1 (en)
JP (2) JP7038100B2 (en)
KR (1) KR102456488B1 (en)
CN (1) CN109952106B (en)
AU (1) AU2017298545B2 (en)
CA (1) CA3030748C (en)
IL (1) IL264306B2 (en)
SG (1) SG11201900442PA (en)
WO (1) WO2018018017A1 (en)

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