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SG11201806002SA - Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process - Google Patents

Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process

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Publication number
SG11201806002SA
SG11201806002SA SG11201806002SA SG11201806002SA SG11201806002SA SG 11201806002S A SG11201806002S A SG 11201806002SA SG 11201806002S A SG11201806002S A SG 11201806002SA SG 11201806002S A SG11201806002S A SG 11201806002SA SG 11201806002S A SG11201806002S A SG 11201806002SA
Authority
SG
Singapore
Prior art keywords
international
california
extending
somatic cells
replicative capacity
Prior art date
Application number
SG11201806002SA
Inventor
Nicholas Genovese
Danielle Nicole Desmet
Eric Schulze
Original Assignee
Memphis Meats Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Memphis Meats Inc filed Critical Memphis Meats Inc
Publication of SG11201806002SA publication Critical patent/SG11201806002SA/en

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
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    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12N9/14—Hydrolases (3)
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    • C12Y207/11—Protein-serine/threonine kinases (2.7.11)
    • C12Y207/11022—Cyclin-dependent kinase (2.7.11.22)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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    • C12N2310/00—Structure or type of the nucleic acid
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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization MD 1111101110101011111 HO 11111 01110111010111110101110110111110111011# International Bureau .. .... ..Yejd ..... .....!;,,, (10) International Publication Number (43) International Publication Date WO 2017/124100 Al 20 July 2017 (20.07.2017) WIPO I PCT (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A23L 1/31 (2006.01) C12N 15/85 (2006.01) kind of national protection available): AE, AG, AL, AM, C12N 5/077 (2010.01) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, (21) International Application Number: DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, PCT/US2017/013782 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, (22) International Filing Date: KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, 17 January 2017 (17.01.2017) MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, (25) Filing Language: English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, (26) Publication Language: English TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/278,869 14 January 2016 (14.01.2016) US (84) Designated States (unless otherwise indicated, for every 62/361,867 13 July 2016 (13.07.2016) US kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, (71) Applicant: MEMPHIS MEATS, INC. [US/US]; 1933 TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Davis Street, Suite 312, San Leandro, California 94577 TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (US). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, (72) Inventors: GENOVESE, Nicholas; 1517 Hayden Street, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Hayward, California 94545 (US). DESMET, Danielle SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, _ Nicole; 1921 Delaware St. #4, Berkeley, California 94709 GW, KM, ML, MR, NE, SN, TD, TG). — (US). SCHULZE, Eric; 821 Leavenworth St., Unit 53, Published: _ = San Francisco, California 94109 (US). — with international search report (Art. 21(3)) (74) Agent: MADHURI, Roy; Cooley LLP, 1299 Pennsylvania with sequence listing of description (Rule 5.2(a)) part Avenue, NW, Suite 700, Washington, DC 20004 (US). = = = = = = = = = = Il © (54) Title: METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO 0 CULTIVATION PROCESS Il 'V (57) : A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amend - \" ments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applica- tions and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first IN exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and O p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental popula - e , ' tions. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase 0 protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application ,..1: is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.
SG11201806002SA 2016-01-14 2017-01-17 Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process SG11201806002SA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662278869P 2016-01-14 2016-01-14
US201662361867P 2016-07-13 2016-07-13
PCT/US2017/013782 WO2017124100A1 (en) 2016-01-14 2017-01-17 Methods for extending the replicative capacity of somatic cells during an ex vivo cultivation process

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SG11201806002SA true SG11201806002SA (en) 2018-08-30

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US (2) US20190024079A1 (en)
EP (1) EP3394246A4 (en)
JP (1) JP2019501657A (en)
KR (1) KR20180134847A (en)
CN (1) CN108779471A (en)
AU (1) AU2017208094A1 (en)
CA (1) CA3011484A1 (en)
MX (1) MX2018008733A (en)
SG (1) SG11201806002SA (en)
WO (1) WO2017124100A1 (en)

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