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SG11201805846TA - Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound - Google Patents

Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound

Info

Publication number
SG11201805846TA
SG11201805846TA SG11201805846TA SG11201805846TA SG11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA SG 11201805846T A SG11201805846T A SG 11201805846TA
Authority
SG
Singapore
Prior art keywords
cells
hepatic
molecular
low
weight compound
Prior art date
Application number
SG11201805846TA
Inventor
Takahiro Ochiya
Takeshi Katsuda
Original Assignee
Nat Cancer Center Japan
Cynity Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nat Cancer Center Japan, Cynity Co Ltd filed Critical Nat Cancer Center Japan
Publication of SG11201805846TA publication Critical patent/SG11201805846TA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

The present invention provides a method for producing hepatic stem/progenitor cells from mammal hepatocytes, comprising bringing a TGFI-receptor inhibitor, and optionally a GSK3 inhibitor and/or a ROCK inhibitor into contact with the hepatocytes in vitro. 36
SG11201805846TA 2016-01-08 2017-01-06 Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound SG11201805846TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016003088 2016-01-08
PCT/JP2017/000342 WO2017119512A1 (en) 2016-01-08 2017-01-06 Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound

Publications (1)

Publication Number Publication Date
SG11201805846TA true SG11201805846TA (en) 2018-08-30

Family

ID=59273713

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201805846TA SG11201805846TA (en) 2016-01-08 2017-01-06 Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound
SG10202006104YA SG10202006104YA (en) 2016-01-08 2017-01-06 Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG10202006104YA SG10202006104YA (en) 2016-01-08 2017-01-06 Method for producing hepatic stem/precursor cells from mature hepatic cells using low-molecular-weight compound

Country Status (13)

Country Link
US (1) US10961507B2 (en)
EP (1) EP3401392B1 (en)
JP (1) JP7063624B2 (en)
KR (1) KR20180114032A (en)
CN (1) CN108779439B (en)
AU (1) AU2017205801A1 (en)
CA (1) CA3010808A1 (en)
DK (1) DK3401392T3 (en)
ES (1) ES2908707T3 (en)
PL (1) PL3401392T3 (en)
RU (1) RU2018128598A (en)
SG (2) SG11201805846TA (en)
WO (1) WO2017119512A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018079714A1 (en) * 2016-10-28 2018-05-03 国立研究開発法人国立がん研究センター Method for preparing human hepatocyte progenitor cells
US20200010807A1 (en) * 2016-11-22 2020-01-09 Kyoto University Stepwise method for inducing cholangiocyte progenitors from hepatoblasts
WO2020080550A1 (en) 2018-10-15 2020-04-23 Cynity株式会社 Method for producing stem/precursor cells, by using low molecular weight compound, from cells derived from endodermal tissue or organ
JP7284985B2 (en) * 2019-03-29 2023-06-01 国立大学法人 長崎大学 Method for producing cell population containing hepatic progenitor cells
CN110013544B (en) * 2019-04-23 2021-04-30 中国人民解放军第二军医大学 Application of small molecule combination in preparing medicine for treating chronic liver injury
KR20240040102A (en) 2021-07-29 2024-03-27 도쿄 메디컬 유니버시티 Method for producing hyperproliferative cells, hyperproliferative cells, and uses thereof
EP4419649A1 (en) * 2021-10-18 2024-08-28 Evia Life Sciences Inc. Compositions and methods of use thereof for treating liver fibrosis
WO2023067394A2 (en) 2021-10-22 2023-04-27 Evia Life Sciences Inc. Methods for making extracellular vesicles, and compositions and methods of use thereof

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JPH08163996A (en) * 1994-12-13 1996-06-25 Sumitomo Bakelite Co Ltd Animal cytotoxicity test method
JPH08308562A (en) * 1995-05-16 1996-11-26 Sumitomo Bakelite Co Ltd Animal cell incubator and method for measuring drug metabolic activity using the same
JP2000189189A (en) 1998-12-24 2000-07-11 Jms Co Ltd Method and apparatus for evaluation test using cell
JP2004198150A (en) 2002-12-17 2004-07-15 Banyu Pharmaceut Co Ltd Method for predicting hepatic metabolic rate of drug and method for predicting drug-drug interaction
JP2006254896A (en) 2005-01-07 2006-09-28 Effector Cell Institute Inc Human hepatocyte-like cell and uses thereof
EP2281875B1 (en) 2005-12-21 2018-03-21 Université Catholique De Louvain Isolated liver stem cells
JP2009521459A (en) 2005-12-22 2009-06-04 ヴェスタ セラピューティクス，インコーポレーテッド Methods using liver progenitor cells in the treatment of liver dysfunction
CN102027105B (en) * 2008-03-17 2015-03-11 斯克里普斯研究所 Combined chemical and genetic approaches for generation of induced pluripotent stem cells
WO2010033906A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Efficient induction of pluripotent stem cells using small molecule compounds
US20100209404A1 (en) * 2009-02-10 2010-08-19 University Of Dayton Enhanced method for producing stem-like cells from somatic cells
CN113621576A (en) 2009-10-16 2021-11-09 斯克里普斯研究所 Induction of pluripotent cells
CA2794473A1 (en) * 2010-02-03 2011-08-11 National Cancer Center Induced hepatic stem cell and process for production thereof, and applications of the cell
CN106893692B (en) * 2010-12-22 2021-11-26 菲特治疗公司 Cell culture platform for single cell sorting and enhanced IPSC reprogramming
JP6124347B2 (en) * 2011-08-02 2017-05-10 石川　哲也 Induction method and production method of hepatic progenitor cells with TGF-β signaling inhibitor and / or Y-27632, and production method of hepatocytes from the hepatic progenitor cells
WO2014025046A1 (en) 2012-08-09 2014-02-13 独立行政法人国立がん研究センター Promoter of differentiation from hepatic progenitor cell into hepatic cell, and use thereof
JP6113999B2 (en) 2012-10-18 2017-04-12 株式会社クラレ Compound screening method
EP2925859B1 (en) * 2012-11-29 2018-05-02 Takara Bio Europe AB Maturation of hepatocyte-like cells derived from human pluripotent stem cells
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Also Published As

Publication number Publication date
JPWO2017119512A1 (en) 2018-11-15
EP3401392A4 (en) 2019-09-18
JP7063624B2 (en) 2022-05-09
DK3401392T3 (en) 2022-03-07
ES2908707T3 (en) 2022-05-03
RU2018128598A (en) 2020-02-11
SG10202006104YA (en) 2020-07-29
CA3010808A1 (en) 2017-07-13
WO2017119512A1 (en) 2017-07-13
AU2017205801A1 (en) 2018-08-16
EP3401392A1 (en) 2018-11-14
PL3401392T3 (en) 2022-06-27
US20190010464A1 (en) 2019-01-10
EP3401392B1 (en) 2022-02-23
KR20180114032A (en) 2018-10-17
US10961507B2 (en) 2021-03-30
CN108779439A (en) 2018-11-09
CN108779439B (en) 2023-05-30

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