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SG11201700296YA - Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof - Google Patents

Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof

Info

Publication number
SG11201700296YA
SG11201700296YA SG11201700296YA SG11201700296YA SG11201700296YA SG 11201700296Y A SG11201700296Y A SG 11201700296YA SG 11201700296Y A SG11201700296Y A SG 11201700296YA SG 11201700296Y A SG11201700296Y A SG 11201700296YA SG 11201700296Y A SG11201700296Y A SG 11201700296YA
Authority
SG
Singapore
Prior art keywords
lnnt
lnt
biotechnological production
fucosylated derivatives
fucosylated
Prior art date
Application number
SG11201700296YA
Inventor
Florian Baumgärtner
Georg A Sprenger
Christoph Albermann
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Publication of SG11201700296YA publication Critical patent/SG11201700296YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01022—Lactose synthase (2.4.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01062—Ganglioside galactosyltransferase (2.4.1.62)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01146—Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (2.4.1.146)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01052—Fucokinase (2.7.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0703—Fucose-1-phosphate guanylyltransferase (2.7.7.30)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07064—UTP-monosaccharide-1-phosphate uridylyltransferase (2.7.7.64)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03002—UDP-glucose 4-epimerase (5.1.3.2), i.e. UDP-galactose 4-epimerase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11201700296YA 2014-07-14 2015-04-10 Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof SG11201700296YA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14176958 2014-07-14
EP14198960 2014-12-18
PCT/EP2015/057805 WO2016008602A1 (en) 2014-07-14 2015-04-10 Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof

Publications (1)

Publication Number Publication Date
SG11201700296YA true SG11201700296YA (en) 2017-02-27

Family

ID=52823645

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201808050YA SG10201808050YA (en) 2014-07-14 2015-04-10 BIOTECHNOLOGICAL PRODUCTION OF LNT, LNnT AND THE FUCOSYLATED DERIVATIVES THEREOF
SG11201700296YA SG11201700296YA (en) 2014-07-14 2015-04-10 Biotechnological production of lnt, lnnt and the fucosylated derivatives thereof

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201808050YA SG10201808050YA (en) 2014-07-14 2015-04-10 BIOTECHNOLOGICAL PRODUCTION OF LNT, LNnT AND THE FUCOSYLATED DERIVATIVES THEREOF

Country Status (14)

Country Link
US (1) US20170204443A1 (en)
EP (1) EP3169775B1 (en)
JP (1) JP2017520267A (en)
KR (1) KR20170028438A (en)
CN (1) CN106536726A (en)
AU (1) AU2015291487A1 (en)
BR (1) BR112017000596A2 (en)
CA (1) CA2954456A1 (en)
IL (1) IL249972A0 (en)
MX (1) MX2017000621A (en)
RU (1) RU2017104525A (en)
SG (2) SG10201808050YA (en)
WO (1) WO2016008602A1 (en)
ZA (1) ZA201700935B (en)

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EP3183349B1 (en) * 2014-08-19 2020-03-25 Purecircle SDN BHD Method for preparing rebaudioside i
EP3445770A4 (en) 2016-04-19 2020-03-18 Glycom A/S SEPARATION OF OLIGOSACCHARIDES FROM FERMENTATION BREW
EP3474861A4 (en) 2016-06-24 2020-04-15 Glycom A/S COMPOSITIONS WITH HMOS, THEIR PRODUCTION AND USE FOR THE PREVENTION AND / OR TREATMENT OF VIRAL AND / OR BACTERIAL INFECTIONS
US10407516B2 (en) 2016-10-31 2019-09-10 Glycom A/S Crystalline HMO
EP3645145A4 (en) 2017-06-30 2021-04-28 Glycom A/S CLEANING OLIGOSACCHARIDES
EP3425052A1 (en) * 2017-07-07 2019-01-09 Jennewein Biotechnologie GmbH Fucosyltransferases and their use in producing fucosylated oligosaccharides
CN119655445A (en) 2017-07-12 2025-03-21 格礼卡姆股份公司 Amorphous mixture comprising neutral mono-or oligosaccharides and an acidic non-carbohydrate component
CN108410787A (en) * 2018-03-13 2018-08-17 光明乳业股份有限公司 A kind of recombined bacillus subtilis of synthesis new tetroses of lactoyl-N- and its construction method and application
BR112021010116A2 (en) * 2018-12-04 2021-08-31 Glycom A/S SYNTHESIS OF FUCOSYLATED OLIGOSACCHARIDE LNFP-V
CN109735479B (en) * 2019-01-30 2022-04-01 光明乳业股份有限公司 Recombinant bacillus subtilis for synthesizing 2' -fucosyllactose and construction method and application thereof
CN109735480B (en) * 2019-02-27 2021-11-12 光明乳业股份有限公司 Recombinant bacillus subtilis for synthesizing lactyl-N-neotetraose and construction method and application thereof
CN109825465B (en) * 2019-02-27 2021-11-12 光明乳业股份有限公司 Recombinant bacillus subtilis for synthesizing lactyl-N-neotetraose based on balanced UDP-sugar supply and construction method and application thereof
WO2021122687A1 (en) 2019-12-19 2021-06-24 Basf Se Increasing space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility in the production of fine chemicals
WO2021199445A1 (en) * 2020-04-03 2021-10-07 Rensselaer Polytechnic Institute Method for producing sulfated polysaccharide and method for producing paps
CN111979168B (en) * 2020-08-17 2022-07-22 江南大学 Genetic engineering bacterium for improving yield of lactoyl-N-trisaccharide II and production method
US20250163091A1 (en) * 2022-01-19 2025-05-22 The Regents Of The University Of California Functionalized human milk oligosaccharides and methods for producing them
WO2023168441A1 (en) * 2022-03-04 2023-09-07 The Regents Of The University Of California Production of milk oligosaccharides in plants
WO2023219437A1 (en) * 2022-05-11 2023-11-16 (주)에이피테크놀로지 Method for producing lacto-n-tetraose and lacto-n-neotetraose using corynebacterium glutamicum
KR102645729B1 (en) * 2022-05-11 2024-03-12 (주)에이피테크놀로지 Method for producing Lacto-N-tetraose and Lacto-N-neotetraose using Corynebacterium glutamicum
CN115094103A (en) * 2022-07-22 2022-09-23 南宁东恒华道生物科技有限责任公司 Novel process for producing galactose by combined enzyme method by taking sucrose, starch or glycogen as raw materials
CN118056902B (en) * 2022-11-21 2025-02-11 山东恒鲁生物科技有限公司 Synthetic Mature Peptide Sequences of HMOs and Their Applications

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CN100342025C (en) * 1996-09-17 2007-10-10 协和发酵工业株式会社 Processes for producing sugar nucleotides and complex carbohydrates
JPH11221079A (en) * 1998-02-04 1999-08-17 Kyowa Hakko Kogyo Co Ltd Glycosyltransferase and DNA encoding the enzyme
AU5706000A (en) * 1999-06-29 2001-01-31 Kyowa Hakko Kogyo Co. Ltd. Useful polypeptide
FR2796082B1 (en) * 1999-07-07 2003-06-27 Centre Nat Rech Scient PROCESS FOR PRODUCING OLIGOSACCHARIDES
AU2001284418B2 (en) * 2000-09-01 2007-11-08 Kyowa Hakko Kirin Co., Ltd. Novel polypeptide
JP3890556B2 (en) * 2001-11-28 2007-03-07 富士フイルム株式会社 Nucleic acid separation and purification method and nucleic acid separation and purification unit
MY183602A (en) * 2008-12-19 2021-03-02 Chr Hansen Hmo Gmbh Synthesis of fucosylated compounds
EP2454948A1 (en) * 2010-11-23 2012-05-23 Nestec S.A. Oligosaccharide mixture and food product comprising this mixture, especially infant formula
EP2675899A4 (en) * 2011-02-16 2014-09-10 Glycosyn LLC BIOSYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES IN MANIPULATED BACTERIA
ES2671555T3 (en) * 2011-06-07 2018-06-07 Hero Ag Obtaining oligosaccharides through a biotechnological process
EP2859112A4 (en) * 2012-06-08 2015-10-28 Glycom As Method for producing oligosaccharides and oligosaccharide glycosides by fermentation
CA2904091A1 (en) * 2013-03-14 2014-09-25 Glycosyn LLC Microorganisms and methods for producing sialylated and n-acetylglucosamine-containing oligosaccharides

Also Published As

Publication number Publication date
US20170204443A1 (en) 2017-07-20
EP3169775A1 (en) 2017-05-24
RU2017104525A (en) 2018-08-14
RU2017104525A3 (en) 2018-12-12
WO2016008602A1 (en) 2016-01-21
ZA201700935B (en) 2018-08-29
CA2954456A1 (en) 2016-01-21
JP2017520267A (en) 2017-07-27
IL249972A0 (en) 2017-03-30
AU2015291487A1 (en) 2017-02-16
CN106536726A (en) 2017-03-22
MX2017000621A (en) 2017-04-27
SG10201808050YA (en) 2018-10-30
KR20170028438A (en) 2017-03-13
EP3169775B1 (en) 2025-07-30
BR112017000596A2 (en) 2018-01-23

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