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SG11201608585RA - Modified transposases for improved insertion sequence bias and increased dna input tolerance - Google Patents

Modified transposases for improved insertion sequence bias and increased dna input tolerance

Info

Publication number
SG11201608585RA
SG11201608585RA SG11201608585RA SG11201608585RA SG11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA SG 11201608585R A SG11201608585R A SG 11201608585RA
Authority
SG
Singapore
Prior art keywords
insertion sequence
increased dna
dna input
improved insertion
sequence bias
Prior art date
Application number
SG11201608585RA
Inventor
Christian Gloeckner
Amirali Kia
Erin Bomati
Molly He
Haiying Li Grunenwald
Scott Kuersten
Trina Faye Osothprarop
Darin Haskins
Joshua Burgess
Anupama Khanna
Daniel Schlingman
Ramesh Vaidyanathan
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of SG11201608585RA publication Critical patent/SG11201608585RA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201608585RA 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance SG11201608585RA (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461979871P 2014-04-15 2014-04-15
US201462062006P 2014-10-09 2014-10-09
US201462080882P 2014-11-17 2014-11-17
PCT/US2015/025889 WO2015160895A2 (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

Publications (1)

Publication Number Publication Date
SG11201608585RA true SG11201608585RA (en) 2016-11-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201608585RA SG11201608585RA (en) 2014-04-15 2015-04-15 Modified transposases for improved insertion sequence bias and increased dna input tolerance

Country Status (9)

Country Link
US (4) US9790476B2 (en)
EP (2) EP3132029A2 (en)
JP (1) JP6490710B2 (en)
CN (3) CN120098956A (en)
AU (3) AU2015247779B2 (en)
CA (1) CA2946046A1 (en)
SG (1) SG11201608585RA (en)
TW (1) TWI682997B (en)
WO (1) WO2015160895A2 (en)

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US20180298356A1 (en) 2018-10-18
AU2015247779B2 (en) 2021-06-24
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