SG10201912516SA - Multiplexed single cell gene expression analysis using template switch and tagmentation - Google Patents
Multiplexed single cell gene expression analysis using template switch and tagmentationInfo
- Publication number
- SG10201912516SA SG10201912516SA SG10201912516SA SG10201912516SA SG10201912516SA SG 10201912516S A SG10201912516S A SG 10201912516SA SG 10201912516S A SG10201912516S A SG 10201912516SA SG 10201912516S A SG10201912516S A SG 10201912516SA SG 10201912516S A SG10201912516S A SG 10201912516SA
- Authority
- SG
- Singapore
- Prior art keywords
- tagmentation
- gene expression
- single cell
- expression analysis
- cell gene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985983P | 2014-04-29 | 2014-04-29 | |
| US201461987433P | 2014-05-01 | 2014-05-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201912516SA true SG10201912516SA (en) | 2020-02-27 |
Family
ID=53055134
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| SG11201609053YA SG11201609053YA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201609053YA SG11201609053YA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US20170044525A1 (en) |
| EP (2) | EP3680333A1 (en) |
| JP (1) | JP6412954B2 (en) |
| CN (1) | CN106459967A (en) |
| AU (4) | AU2015253299B2 (en) |
| CA (2) | CA2947426C (en) |
| SG (2) | SG10201912516SA (en) |
| WO (1) | WO2015168161A2 (en) |
Families Citing this family (117)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| EP2739587B1 (en) | 2011-08-01 | 2020-05-27 | Denovo Sciences | Cell capture system |
| US10466160B2 (en) | 2011-08-01 | 2019-11-05 | Celsee Diagnostics, Inc. | System and method for retrieving and analyzing particles |
| US9404864B2 (en) | 2013-03-13 | 2016-08-02 | Denovo Sciences, Inc. | System for imaging captured cells |
| JP6375230B2 (en) | 2012-02-27 | 2018-08-15 | セルラー リサーチ， インコーポレイテッド | Compositions and kits for molecular counting |
| US9752181B2 (en) | 2013-01-26 | 2017-09-05 | Denovo Sciences, Inc. | System and method for capturing and analyzing cells |
| US9707562B2 (en) | 2013-03-13 | 2017-07-18 | Denovo Sciences, Inc. | System for capturing and analyzing cells |
| US10391490B2 (en) | 2013-05-31 | 2019-08-27 | Celsee Diagnostics, Inc. | System and method for isolating and analyzing cells |
| US9856535B2 (en) | 2013-05-31 | 2018-01-02 | Denovo Sciences, Inc. | System for isolating cells |
| EP3039158B1 (en) | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| US10975371B2 (en) | 2014-04-29 | 2021-04-13 | Illumina, Inc. | Nucleic acid sequence analysis from single cells |
| WO2016090148A1 (en) | 2014-12-03 | 2016-06-09 | IsoPlexis Corporation | Analysis and screening of cell secretion profiles |
| ES2836802T3 (en) | 2015-02-27 | 2021-06-28 | Becton Dickinson Co | Spatially addressable molecular barcodes |
| CN107406888A (en) | 2015-03-30 | 2017-11-28 | 赛卢拉研究公司 | Methods and compositions for combining barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| SG10201912283RA (en) * | 2015-08-28 | 2020-02-27 | Illumina Inc | Nucleic acid sequence analysis from single cells |
| WO2017044574A1 (en) | 2015-09-11 | 2017-03-16 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
| EP3350732B1 (en) * | 2015-09-15 | 2024-07-24 | Takara Bio USA, Inc. | Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same |
| CN105926043B (en) * | 2016-04-19 | 2018-08-28 | 苏州贝康医疗器械有限公司 | A method of improving fetus dissociative DNA accounting in pregnant woman blood plasma dissociative DNA sequencing library |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| WO2017204294A1 (en) * | 2016-05-25 | 2017-11-30 | 株式会社ニコン | Target biological molecule identification method, beads for target biological molecule identification use, set of beads, and target biological molecule identification device |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US11293021B1 (en) | 2016-06-23 | 2022-04-05 | Inscripta, Inc. | Automated cell processing methods, modules, instruments, and systems |
| WO2018031631A1 (en) * | 2016-08-10 | 2018-02-15 | President And Fellows Of Harvard College | Methods of de novo assembly of barcoded genomic dna fragments |
| EP3510398A1 (en) | 2016-09-12 | 2019-07-17 | Isoplexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| EP4300099A3 (en) | 2016-09-26 | 2024-03-27 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| EP3529400B1 (en) | 2016-10-24 | 2021-02-17 | Geneinfosec, Inc. | Concealing information present within nucleic acids |
| WO2018089232A1 (en) * | 2016-11-09 | 2018-05-17 | Qiagen Sciences Llc | Enhancing sequencing performance in sequencing-by-synthesis |
| JP7348066B2 (en) | 2016-11-11 | 2023-09-20 | アイソプレキシス コーポレイション | Compositions and methods for simultaneous analysis of single cell genome, transcriptome and proteome |
| EP3559255A1 (en) | 2016-12-23 | 2019-10-30 | Grail, Inc. | Methods for high efficiency library preparation using double-stranded adapters |
| US10711269B2 (en) * | 2017-01-18 | 2020-07-14 | Agilent Technologies, Inc. | Method for making an asymmetrically-tagged sequencing library |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| EP3583212A4 (en) * | 2017-02-16 | 2021-01-13 | Takara Bio USA, Inc. | METHOD FOR PREPARING NUCLEIC ACID LIBRARIES AND COMPOSITIONS AND KITS FOR CARRYING OUT THEREOF |
| US11274344B2 (en) | 2017-03-30 | 2022-03-15 | Grail, Inc. | Enhanced ligation in sequencing library preparation |
| WO2018183897A1 (en) | 2017-03-31 | 2018-10-04 | Grail, Inc. | Higher target capture efficiency using probe extension |
| EP4345172A3 (en) | 2017-06-05 | 2024-07-03 | Becton, Dickinson and Company | Sample indexing for single cells |
| CN107502607A (en) * | 2017-06-20 | 2017-12-22 | 浙江大学 | A kind of a large amount of tissues, cell sample mRNA molecular barcode mark, library construction, the method for sequencing |
| JP7160349B2 (en) * | 2017-06-23 | 2022-10-25 | 国立研究開発法人理化学研究所 | Methods of sequencing and analyzing nucleic acids |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US20190002874A1 (en) | 2017-06-30 | 2019-01-03 | Inscripta, Inc. | Cell libraries created using rationally designed nucleic acids |
| CN112980667B (en) | 2017-08-29 | 2024-09-17 | 伯乐实验室有限公司 | Systems and methods for isolating and analyzing cells |
| WO2019061196A1 (en) * | 2017-09-28 | 2019-04-04 | 深圳华大生命科学研究院 | Pcr primers for detection and use thereof |
| JP2021509024A (en) * | 2018-01-02 | 2021-03-18 | ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン | Capturing a large number of droplets |
| WO2019191459A1 (en) * | 2018-03-28 | 2019-10-03 | Berkeley Lights, Inc. | Methods for preparation of nucleic acid sequencing libraries |
| US10557216B2 (en) | 2018-04-24 | 2020-02-11 | Inscripta, Inc. | Automated instrumentation for production of T-cell receptor peptide libraries |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US20210079459A1 (en) * | 2018-05-01 | 2021-03-18 | Takara Bio Usa, Inc. | Methods of Amplifying Nucleic Acids and Compositions and Kits for Practicing the Same |
| US11773441B2 (en) | 2018-05-03 | 2023-10-03 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| US11365409B2 (en) | 2018-05-03 | 2022-06-21 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
| CN108611346A (en) * | 2018-05-06 | 2018-10-02 | 湖南大地同年生物科技有限公司 | A kind of construction method in unicellular gene expression amount detection library |
| AU2019292919A1 (en) | 2018-06-30 | 2021-03-11 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| WO2020014303A1 (en) * | 2018-07-11 | 2020-01-16 | X Gen Us Co. | Transposome enabled dna/rna-sequencing (ted rna-seq) |
| KR102893591B1 (en) | 2018-08-15 | 2025-12-02 | 일루미나, 인코포레이티드 | Compositions and methods for improving library enrichment |
| CN110835783B (en) * | 2018-08-17 | 2023-06-20 | 深圳华大生命科学研究院 | Construction method, sequencing method and reagent for long-reading long-length high-quality sequencing nucleic acid library |
| EP4249651B1 (en) | 2018-08-20 | 2025-01-29 | Bio-Rad Laboratories, Inc. | Nucleotide sequence generation by barcode bead-colocalization in partitions |
| JP7670607B2 (en) | 2018-10-01 | 2025-04-30 | ベクトン・ディキンソン・アンド・カンパニー | Determination of 5' transcript sequence |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| JP7554121B2 (en) | 2018-11-01 | 2024-09-19 | イルミナ インコーポレイテッド | Methods and Compositions for Somatic Variant Detection |
| EP3877520B1 (en) | 2018-11-08 | 2025-03-19 | Becton, Dickson And Company | Whole transcriptome analysis of single cells using random priming |
| AU2019390388B2 (en) * | 2018-11-30 | 2025-08-07 | Illumina, Inc. | Analysis of multiple analytes using a single assay |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| SG11202102424QA (en) * | 2018-12-17 | 2021-04-29 | Illumina Inc | Methods and means for preparing a library for sequencing |
| MY205579A (en) * | 2018-12-21 | 2024-10-28 | Illumina Inc | Nuclease-based rna depletion |
| EP3902922A1 (en) * | 2018-12-28 | 2021-11-03 | Biobloxx AB | Method and kit for preparing complementary dna |
| WO2020154247A1 (en) | 2019-01-23 | 2020-07-30 | Cellular Research, Inc. | Oligonucleotides associated with antibodies |
| EP3924506A1 (en) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybrid targeted and whole transcriptome amplification |
| US10815467B2 (en) | 2019-03-25 | 2020-10-27 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| CN113795591A (en) * | 2019-04-02 | 2021-12-14 | 使命生物公司 | Methods and systems for characterizing tumors and identifying tumor heterogeneity |
| US10633693B1 (en) | 2019-04-16 | 2020-04-28 | Celsee Diagnostics, Inc. | System and method for leakage control in a particle capture system |
| US11273439B2 (en) | 2019-05-07 | 2022-03-15 | Bio-Rad Laboratories, Inc. | System and method for target material retrieval from microwells |
| WO2020227309A1 (en) | 2019-05-07 | 2020-11-12 | Bio-Rad Laboratories, Inc. | System and method for automated single cell processing |
| EP3953477A4 (en) | 2019-06-06 | 2022-06-22 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
| KR20250084977A (en) | 2019-06-14 | 2025-06-11 | 바이오 래드 래버러토리스 인코오포레이티드 | System and method for automated single cell processing and analyses |
| CN110257479A (en) * | 2019-06-25 | 2019-09-20 | 北京全式金生物技术有限公司 | A kind of method that rapid build RNA 3 ' holds gene expression library |
| CN114051534B (en) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | Single-cell chromatin immunoprecipitation sequencing assay |
| JP7304773B2 (en) * | 2019-08-27 | 2023-07-07 | 株式会社トプコン | Simulated blood flow generator and simulated blood flow generation method |
| AU2020342793A1 (en) * | 2019-09-06 | 2022-04-21 | Cemm - Forschungszentrum Für Molekulare Medizin Gmbh | Method for sequencing RNA oligonucleotides |
| US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
| WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| KR20220118498A (en) | 2019-12-18 | 2022-08-25 | 인스크립타 인코포레이티드 | Cascade/DCAS3 Complementary Assay for In Vivo Detection of Nucleic Acid-Guided Nuclease Edited Cells |
| WO2021133842A1 (en) | 2019-12-23 | 2021-07-01 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
| CN114901831B (en) * | 2020-01-23 | 2025-03-04 | 青岛华大智造科技有限责任公司 | Single-cell sequencing based on droplet microfluidics and its applications |
| US20210230681A1 (en) | 2020-01-24 | 2021-07-29 | 10X Genomics, Inc. | Methods for spatial analysis using proximity ligation |
| KR20220133257A (en) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | Electroporation modules and instruments |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| US11504719B2 (en) | 2020-03-12 | 2022-11-22 | Bio-Rad Laboratories, Inc. | System and method for receiving and delivering a fluid for sample processing |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| WO2021231779A1 (en) | 2020-05-14 | 2021-11-18 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| WO2021247593A1 (en) | 2020-06-02 | 2021-12-09 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US12265079B1 (en) | 2020-06-02 | 2025-04-01 | 10X Genomics, Inc. | Systems and methods for detecting analytes from captured single biological particles |
| US20210381007A1 (en) * | 2020-06-04 | 2021-12-09 | Inscripta, Inc. | Methods and compositions for crispr editing of cells and correlating the edits to a resulting cellular nucleic acid profile |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022021279A1 (en) * | 2020-07-31 | 2022-02-03 | 北京寻因生物科技有限公司 | Multi-nucleic acid co-labeling support, preparation method therefor, and application thereof |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| BR112023002051A2 (en) | 2020-08-06 | 2023-04-18 | Illumina Inc | PREPARATION OF RNA AND DNA SEQUENCING LIBRARIES USING MICROSPHERE-ATTACHED TRANSPOSOMES |
| EP4214314A4 (en) | 2020-09-15 | 2024-10-16 | Inscripta, Inc. | CRISPR EDITING TO INCORPORATE NUCLEIC ACID DOCKING PLATES INTO LIVING CELL GENOMES |
| WO2022067494A1 (en) * | 2020-09-29 | 2022-04-07 | Singleron (Nanjing) Biotechnologies, Ltd. | Method for detection of whole transcriptome in single cells |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| WO2022132206A1 (en) | 2020-12-15 | 2022-06-23 | Becton, Dickinson And Company | Single cell secretome analysis |
| WO2022146497A1 (en) | 2021-01-04 | 2022-07-07 | Inscripta, Inc. | Mad nucleases |
| US20240376451A1 (en) | 2021-01-07 | 2024-11-14 | Inscripta, Inc. | Mad nucleases |
| CA3203805A1 (en) | 2021-01-08 | 2022-07-14 | Cellanome, Inc. | Devices and methods for analyzing biological samples |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| WO2022188054A1 (en) * | 2021-03-10 | 2022-09-15 | Nanjing University | Methods and reagents for sample multiplexing for high throughput single-cell rna sequencing |
| JP2024511760A (en) | 2021-03-31 | 2024-03-15 | イルミナ インコーポレイテッド | Method for preparing directional tagmentation sequencing libraries using transposon-based technology with unique molecular identifiers for error correction |
| JP2024520804A (en) * | 2021-06-11 | 2024-05-24 | セラノーム， インコーポレイテッド | Systems and methods for analyzing biological samples - Patents.com |
| EP4444910A4 (en) | 2021-12-10 | 2025-11-05 | Bio Rad Laboratories Inc | COMPOSITIONS, METHOD AND SYSTEMS FOR SAMPLE PROCESSING WITH MORPHOLOGICALLY ADAPTABLE FUNCTIONALIZED PARTICLES |
| EP4484574A4 (en) * | 2022-02-24 | 2025-12-03 | Univ Zhejiang | SINGLE-CELL TRANSCRIPTOM SEQUALIZATION METHODS AND USE OF THEM |
| WO2024254003A1 (en) | 2023-06-05 | 2024-12-12 | Illumina, Inc. | Identification and mapping of methylation sites |
| WO2025128786A1 (en) * | 2023-12-12 | 2025-06-19 | Baylor College Of Medicine | Methods for simultaneous characterization of genome and transcriptome of a single cell |
| WO2025145050A2 (en) * | 2023-12-29 | 2025-07-03 | Illumina, Inc. | Reusable surface chemistry for spatial substrates |
Family Cites Families (75)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2044616A1 (en) | 1989-10-26 | 1991-04-27 | Roger Y. Tsien | Dna sequencing |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| WO1997014028A2 (en) | 1995-10-11 | 1997-04-17 | Luminex Corporation | Multiplexed analysis of clinical specimens apparatus and method |
| CA2260991C (en) | 1997-11-18 | 2009-03-24 | Bio-Rad Laboratories, Inc. | Multiplex flow immunoassays with magnetic particles as solid phase |
| US6565727B1 (en) | 1999-01-25 | 2003-05-20 | Nanolytics, Inc. | Actuators for microfluidics without moving parts |
| US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
| US6277607B1 (en) * | 1999-05-24 | 2001-08-21 | Sanjay Tyagi | High specificity primers, amplification methods and kits |
| DE60027576T2 (en) | 1999-08-17 | 2007-05-03 | Luminex Corp., Austin | SECULATION OF FLUORESCENT PARTICLES |
| EP1204869B1 (en) | 1999-08-17 | 2008-10-22 | Luminex Corporation | Method for analyzing a number of samples from a variety of sources for a single analyte |
| DE60131194T2 (en) | 2000-07-07 | 2008-08-07 | Visigen Biotechnologies, Inc., Bellaire | SEQUENCE PROVISION IN REAL TIME |
| WO2002007503A1 (en) | 2000-07-25 | 2002-01-31 | The Regents Of The University Of California | Electrowetting-driven micropumping |
| US6773566B2 (en) | 2000-08-31 | 2004-08-10 | Nanolytics, Inc. | Electrostatic actuators for microfluidics and methods for using same |
| WO2002044425A2 (en) | 2000-12-01 | 2002-06-06 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| WO2003045556A2 (en) | 2001-11-26 | 2003-06-05 | Keck Graduate Institute | Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| FR2841063B1 (en) | 2002-06-18 | 2004-09-17 | Commissariat Energie Atomique | DEVICE FOR DISPLACING SMALL VOLUMES OF LIQUID ALONG A MICRO-CATENARY BY ELECTROSTATIC FORCES |
| EP2607369B1 (en) | 2002-08-23 | 2015-09-23 | Illumina Cambridge Limited | Modified nucleotides for polynucleotide sequencing |
| US6911132B2 (en) | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
| US7547380B2 (en) | 2003-01-13 | 2009-06-16 | North Carolina State University | Droplet transportation devices and methods having a fluid surface |
| CA2528577C (en) * | 2003-06-20 | 2012-08-07 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| US7470397B2 (en) | 2003-10-24 | 2008-12-30 | Adhesives Research, Inc. | Disintegratable films for diagnostic devices |
| ATE434131T1 (en) | 2003-11-17 | 2009-07-15 | Koninkl Philips Electronics Nv | SYSTEM FOR HANDLING A QUANTITY OF FLUID |
| GB0400584D0 (en) | 2004-01-12 | 2004-02-11 | Solexa Ltd | Nucleic acid chacterisation |
| JP2007518991A (en) | 2004-01-14 | 2007-07-12 | ルミネックス・コーポレーション | Method and system for expanding dynamic range |
| FR2866493B1 (en) | 2004-02-16 | 2010-08-20 | Commissariat Energie Atomique | DEVICE FOR CONTROLLING THE DISPLACEMENT OF A DROP BETWEEN TWO OR MORE SOLID SUBSTRATES |
| FR2872715B1 (en) | 2004-07-08 | 2006-11-17 | Commissariat Energie Atomique | MICROREACTOR DROP |
| FR2872809B1 (en) | 2004-07-09 | 2006-09-15 | Commissariat Energie Atomique | METHOD OF ADDRESSING ELECTRODES |
| JP2006058031A (en) | 2004-08-17 | 2006-03-02 | Hitachi High-Technologies Corp | Chemical analyzer |
| JP2008513782A (en) | 2004-09-17 | 2008-05-01 | パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド | Apparatus and method for molecular analysis |
| EP1842211B1 (en) | 2005-01-20 | 2010-08-04 | Luminex Corporation | Microspheres having fluorescent and magnetic properties |
| US7458661B2 (en) | 2005-01-25 | 2008-12-02 | The Regents Of The University Of California | Method and apparatus for promoting the complete transfer of liquid drops from a nozzle |
| DK1859330T3 (en) | 2005-01-28 | 2012-10-15 | Univ Duke | DEVICES AND METHODS OF HANDLING SMALL DROPS ON A PRINTED CIRCUIT CARD |
| US20070023292A1 (en) | 2005-07-26 | 2007-02-01 | The Regents Of The University Of California | Small object moving on printed circuit board |
| WO2007035840A2 (en) | 2005-09-21 | 2007-03-29 | Luminex Corporation | Methods and systems for image data processing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| US20070207513A1 (en) | 2006-03-03 | 2007-09-06 | Luminex Corporation | Methods, Products, and Kits for Identifying an Analyte in a Sample |
| EP3722409A1 (en) | 2006-03-31 | 2020-10-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| CA2680532C (en) | 2006-04-18 | 2017-03-21 | Advanced Liquid Logic, Inc. | Droplet-based pyrosequencing |
| WO2010027894A2 (en) | 2008-08-27 | 2010-03-11 | Advanced Liquid Logic, Inc. | Droplet actuators, modified fluids and methods |
| US7439014B2 (en) | 2006-04-18 | 2008-10-21 | Advanced Liquid Logic, Inc. | Droplet-based surface modification and washing |
| US7901947B2 (en) | 2006-04-18 | 2011-03-08 | Advanced Liquid Logic, Inc. | Droplet-based particle sorting |
| JP4881950B2 (en) | 2006-07-10 | 2012-02-22 | 株式会社日立ハイテクノロジーズ | Liquid transport device |
| US8399188B2 (en) | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| WO2008055256A2 (en) | 2006-11-02 | 2008-05-08 | The Regents Of The University Of California | Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip |
| BRPI0721095B1 (en) | 2006-12-13 | 2015-09-29 | Luminex Corporation | SYSTEMS AND METHODS FOR MULTIPLEX REAL-TIME PCR ANALYSIS |
| WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| WO2008098236A2 (en) | 2007-02-09 | 2008-08-14 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods employing magnetic beads |
| EP2109774B1 (en) | 2007-02-15 | 2018-07-04 | Advanced Liquid Logic, Inc. | Capacitance detection in a droplet actuator |
| US20100048410A1 (en) | 2007-03-22 | 2010-02-25 | Advanced Liquid Logic, Inc. | Bead Sorting on a Droplet Actuator |
| US8093062B2 (en) | 2007-03-22 | 2012-01-10 | Theodore Winger | Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil |
| US20100130369A1 (en) | 2007-04-23 | 2010-05-27 | Advanced Liquid Logic, Inc. | Bead-Based Multiplexed Analytical Methods and Instrumentation |
| US20080283414A1 (en) | 2007-05-17 | 2008-11-20 | Monroe Charles W | Electrowetting devices |
| US8926811B2 (en) | 2007-06-27 | 2015-01-06 | Digital Biosystems | Digital microfluidics based apparatus for heat-exchanging chemical processes |
| US20110303542A1 (en) | 2007-08-08 | 2011-12-15 | Advanced Liquid Logic, Inc. | Use of Additives for Enhancing Droplet Operations |
| US8093064B2 (en) | 2008-05-15 | 2012-01-10 | The Regents Of The University Of California | Method for using magnetic particles in droplet microfluidics |
| US9080211B2 (en) * | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| CN102264914B (en) | 2008-10-24 | 2015-08-12 | 阿霹震中科技公司 | Transposon end compositions and methods for modifying nucleic acids |
| WO2010077859A2 (en) * | 2008-12-15 | 2010-07-08 | Advanced Liquid Logic, Inc. | Nucleic acid amplification and sequencing on a droplet actuator |
| ES2706227T3 (en) | 2009-03-30 | 2019-03-27 | Illumina Inc | Analysis of gene expression in individual cells |
| WO2011002957A2 (en) | 2009-07-01 | 2011-01-06 | Advanced Liquid Logic, Inc. | Droplet actuator devices and methods |
| GB2512213B (en) | 2010-10-08 | 2015-02-11 | Harvard College | High-throughput single cell barcoding |
| US9284602B2 (en) * | 2010-10-27 | 2016-03-15 | President And Fellows Of Harvard College | Compositions of toehold primer duplexes and methods of use |
| HUE068153T2 (en) | 2011-04-15 | 2024-12-28 | Univ Johns Hopkins | Safe sequencing system |
| US9005935B2 (en) | 2011-05-23 | 2015-04-14 | Agilent Technologies, Inc. | Methods and compositions for DNA fragmentation and tagging by transposases |
| US8778848B2 (en) | 2011-06-09 | 2014-07-15 | Illumina, Inc. | Patterned flow-cells useful for nucleic acid analysis |
| WO2013085918A1 (en) | 2011-12-05 | 2013-06-13 | The Regents Of The University Of California | Methods and compostions for generating polynucleic acid fragments |
| WO2013117595A2 (en) | 2012-02-07 | 2013-08-15 | Illumina Cambridge Limited | Targeted enrichment and amplification of nucleic acids on a support |
| NO2694769T3 (en) | 2012-03-06 | 2018-03-03 | ||
| CA2875695C (en) * | 2012-06-15 | 2022-11-15 | The Board Of Regents Of The University Of Texas System | High throughput sequencing of multiple transcripts of a single cell |
| WO2014047561A1 (en) * | 2012-09-21 | 2014-03-27 | The Broad Institute Inc. | Compositions and methods for labeling of agents |
| US9410173B2 (en) * | 2012-10-24 | 2016-08-09 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
| WO2014201273A1 (en) * | 2013-06-12 | 2014-12-18 | The Broad Institute, Inc. | High-throughput rna-seq |
| EP3039158B1 (en) * | 2013-08-28 | 2018-11-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| WO2015103339A1 (en) * | 2013-12-30 | 2015-07-09 | Atreca, Inc. | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
-
2015
- 2015-04-28 SG SG10201912516SA patent/SG10201912516SA/en unknown
- 2015-04-28 CN CN201580033171.7A patent/CN106459967A/en active Pending
- 2015-04-28 SG SG11201609053YA patent/SG11201609053YA/en unknown
- 2015-04-28 WO PCT/US2015/028062 patent/WO2015168161A2/en not_active Ceased
- 2015-04-28 CA CA2947426A patent/CA2947426C/en active Active
- 2015-04-28 EP EP20154378.2A patent/EP3680333A1/en active Pending
- 2015-04-28 CA CA3060708A patent/CA3060708A1/en active Pending
- 2015-04-28 AU AU2015253299A patent/AU2015253299B2/en not_active Ceased
- 2015-04-28 JP JP2016565421A patent/JP6412954B2/en active Active
- 2015-04-28 EP EP15721102.0A patent/EP3137601B1/en active Active
- 2015-04-28 US US15/307,329 patent/US20170044525A1/en not_active Abandoned
-
2018
- 2018-09-07 AU AU2018226493A patent/AU2018226493B2/en not_active Ceased
-
2020
- 2020-10-23 US US17/078,900 patent/US20210079386A1/en active Pending
-
2021
- 2021-05-20 AU AU2021203246A patent/AU2021203246B2/en not_active Ceased
-
2024
- 2024-02-28 AU AU2024201323A patent/AU2024201323A1/en not_active Abandoned
- 2024-08-06 US US18/795,512 patent/US20240392284A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3137601A2 (en) | 2017-03-08 |
| EP3137601B1 (en) | 2020-04-08 |
| AU2018226493A1 (en) | 2018-09-27 |
| CA2947426C (en) | 2020-01-07 |
| JP2017514487A (en) | 2017-06-08 |
| JP6412954B2 (en) | 2018-10-24 |
| SG11201609053YA (en) | 2016-11-29 |
| WO2015168161A2 (en) | 2015-11-05 |
| US20210079386A1 (en) | 2021-03-18 |
| AU2018226493B2 (en) | 2021-02-25 |
| CA3060708A1 (en) | 2015-11-05 |
| AU2024201323A1 (en) | 2024-03-21 |
| AU2021203246A1 (en) | 2021-06-10 |
| EP3680333A1 (en) | 2020-07-15 |
| AU2021203246B2 (en) | 2023-11-30 |
| AU2015253299B2 (en) | 2018-06-14 |
| US20240392284A1 (en) | 2024-11-28 |
| CN106459967A (en) | 2017-02-22 |
| US20170044525A1 (en) | 2017-02-16 |
| CA2947426A1 (en) | 2015-11-05 |
| AU2015253299A1 (en) | 2016-11-17 |
| WO2015168161A3 (en) | 2015-12-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG10201912516SA (en) | Multiplexed single cell gene expression analysis using template switch and tagmentation | |
| GB2556436B (en) | Cell population analysis | |
| IL255157A0 (en) | Microfluidic cell culture | |
| GB2598259B (en) | Cell Population Analysis | |
| GB201611982D0 (en) | Cell culture | |
| SG11201703493SA (en) | Cell culture insert | |
| PL3113883T3 (en) | Cell sorting system using microfabricated components | |
| PL3134724T3 (en) | Electrochemical cell | |
| PL3365107T3 (en) | Cell culture | |
| LT3619319T (en) | Single cell analysis | |
| SG11201702377QA (en) | Blood sample management using open cell foam | |
| SG11201606297UA (en) | Blood sample management using open cell foam | |
| SG11201609055RA (en) | Integrated single cell sequencing | |
| GB201714180D0 (en) | Transduced cell cryoformulation | |
| PL3287521T3 (en) | Cell culture | |
| GB201520210D0 (en) | Electrochemical cell and process | |
| GB201510675D0 (en) | Electrochemical cell and process | |
| GB201410092D0 (en) | Electrolyte membrane | |
| GB201608764D0 (en) | Novel cell culture | |
| GB201604427D0 (en) | Modified cell | |
| TWI563082B (en) | Cell culture device | |
| SG10202010002RA (en) | Cell culture | |
| GB201421342D0 (en) | Cell culture | |
| GB201420709D0 (en) | Electrochemical cells and components thereof | |
| GB201411798D0 (en) | Electrochemical cell |