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SG10201908167YA - Proximity assays for detecting nucleic acids and proteins in a single cell - Google Patents

Proximity assays for detecting nucleic acids and proteins in a single cell

Info

Publication number
SG10201908167YA
SG10201908167YA SG10201908167YA SG10201908167YA SG 10201908167Y A SG10201908167Y A SG 10201908167YA SG 10201908167Y A SG10201908167Y A SG 10201908167YA SG 10201908167Y A SG10201908167Y A SG 10201908167YA
Authority
SG
Singapore
Prior art keywords
proteins
nucleic acids
single cell
detecting nucleic
proximity assays
Prior art date
Application number
Inventor
Camila Egidio
Ramesh Ramakrishnan
David Ruff
Original Assignee
Fluidigm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fluidigm Corp filed Critical Fluidigm Corp
Publication of SG10201908167YA publication Critical patent/SG10201908167YA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROXIMITY ASSAYS FOR DETECTING NUCLEIC ACIDS AND PROTEINS IN A SINGLE CELL Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays. [Figure 1]
SG10201908167Y 2013-09-04 2014-09-04 Proximity assays for detecting nucleic acids and proteins in a single cell SG10201908167YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361873820P 2013-09-04 2013-09-04
US201461987401P 2014-05-01 2014-05-01

Publications (1)

Publication Number Publication Date
SG10201908167YA true SG10201908167YA (en) 2019-10-30

Family

ID=52628934

Family Applications (1)

Application Number Title Priority Date Filing Date
SG10201908167Y SG10201908167YA (en) 2013-09-04 2014-09-04 Proximity assays for detecting nucleic acids and proteins in a single cell

Country Status (6)

Country Link
US (2) US20150132743A1 (en)
EP (1) EP3041957A4 (en)
CN (1) CN105683397A (en)
CA (1) CA2922926A1 (en)
SG (1) SG10201908167YA (en)
WO (1) WO2015035087A1 (en)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008016644A1 (en) 2006-08-01 2008-02-07 Applera Corporation Detection of analytes and nucleic acids
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11560585B2 (en) * 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
CN103703143B (en) 2011-01-31 2016-12-14 爱普瑞斯生物公司 Method for identifying multiple epitopes in a cell
EP2675819B1 (en) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
JP2016514455A (en) 2013-03-15 2016-05-23 フリューダイム・コーポレイション Simultaneous detection of target protein and target nucleic acid in a single cell
ES2711168T3 (en) 2013-08-28 2019-04-30 Becton Dickinson Co Massive parallel analysis of individual cells
JP2017504307A (en) 2013-10-07 2017-02-09 セルラー リサーチ， インコーポレイテッド Method and system for digitally counting features on an array
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
ES2836802T3 (en) 2015-02-27 2021-06-28 Becton Dickinson Co Spatially addressable molecular barcodes
ES2934982T3 (en) 2015-03-30 2023-02-28 Becton Dickinson Co Methods for encoding with combinatorial barcodes
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
JP7129343B2 (en) 2016-05-02 2022-09-01 ベクトン・ディキンソン・アンド・カンパニー Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
CN109154021A (en) * 2016-05-25 2019-01-04 生物辐射实验室股份有限公司 Digital proximity assay
EP3465502B1 (en) 2016-05-26 2024-04-10 Becton, Dickinson and Company Molecular label counting adjustment methods
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US20180119202A1 (en) * 2016-11-01 2018-05-03 University Of Washington Proximity-enhanced nucleic acid-amplified protein detection
KR102790039B1 (en) 2016-11-08 2025-04-04 벡톤 디킨슨 앤드 컴퍼니 Cell marker classification method
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN110573253B (en) 2017-01-13 2021-11-02 赛卢拉研究公司 Hydrophilic coating for fluid channels
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CN107314965B (en) * 2017-04-26 2020-07-31 马鞍山普梅森医学检验实验室有限公司 Sample pretreatment method based on flow type combined ICP-MS single cell protein detection
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11639933B2 (en) 2017-09-27 2023-05-02 Bio-Rad Laboratories, Inc. Digital affinity linkage assay
WO2019126209A1 (en) 2017-12-19 2019-06-27 Cellular Research, Inc. Particles associated with oligonucleotides
US11827922B2 (en) 2018-03-07 2023-11-28 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
CN108693144B (en) * 2018-04-28 2021-02-09 天津大学 Multiplexing single-cell proteomics monitoring method based on SPRM technology
JP7358388B2 (en) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー Molecular barcoding at opposite transcript ends
ES2945191T3 (en) 2018-05-03 2023-06-29 Becton Dickinson Co High-throughput multi-omics sample analysis
EP3856924A4 (en) * 2018-09-26 2022-06-22 Lamprogen, Inc. DIGITAL AMPLIFICATION FOR PROTEIN DETECTION
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
JP7618548B2 (en) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー Whole-transcriptome analysis of single cells using random priming
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN114051534B (en) 2019-07-22 2025-02-21 贝克顿迪金森公司 Single-cell chromatin immunoprecipitation sequencing assay
CA3147077A1 (en) * 2019-08-05 2021-02-11 David Ruff Method and apparatus for single-cell analysis for determining a cell trajectory
JP7522189B2 (en) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー Use of Random Priming to Obtain Full-Length V(D)J Information for Immune Repertoire Sequencing
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US20210230681A1 (en) * 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
CN115335520A (en) 2020-01-29 2022-11-11 贝克顿迪金森公司 Barcoded wells for spatial mapping of single cells by sequencing
US12416043B2 (en) 2020-02-20 2025-09-16 Standard BioTools Inc. Parallelized sample processing and library prep
WO2021168439A1 (en) 2020-02-20 2021-08-26 Fluidigm Corporation Parallelized sample processing and library prep
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
CN115605614A (en) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116635533A (en) 2020-11-20 2023-08-22 贝克顿迪金森公司 Profile analysis of highly expressed and low expressed proteins
US12467081B2 (en) 2020-11-23 2025-11-11 Pleno, Inc. Encoded endonuclease assays
CN116685850A (en) 2020-12-15 2023-09-01 贝克顿迪金森公司 Single-cell secretome analysis
EP4271837A4 (en) 2020-12-31 2025-10-15 Tempus Ai Inc SYSTEMS AND METHODS FOR THE DETECTION OF MULTI-MOLECULAR BIOMARKERS
CN114277090A (en) * 2021-12-17 2022-04-05 宁波熙宁检测技术有限公司 Detection method and detection kit for AAV8 neutralizing antibody
EP4655590A1 (en) * 2023-01-27 2025-12-03 Biomerieux Modified immuno-molecular assay for faster biomarker detection
WO2024254169A1 (en) * 2023-06-06 2024-12-12 Meso Scale Technologies, Llc. Methods for analyzing a sample
WO2025111369A1 (en) * 2023-11-21 2025-05-30 10X Genomics, Inc. Probe-based analysis of polynucleotides and proteins
WO2025199005A1 (en) 2024-03-20 2025-09-25 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Biomarkers for identifying cancer patients at high risk for chemotherapy-induced cardiotoxicity

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5449602A (en) 1988-01-13 1995-09-12 Amoco Corporation Template-directed photoligation
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2240667A1 (en) 1995-12-18 1997-06-26 Washington University Method for nucleic acid analysis using fluorescence resonance energy transfer
EP2574617B1 (en) 1996-02-09 2016-04-20 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
EP2369007B1 (en) 1996-05-29 2015-07-29 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6312892B1 (en) 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US6027998A (en) 1997-12-17 2000-02-22 Advanced Micro Devices, Inc. Method for fully planarized conductive line for a stack gate
ATE397092T1 (en) 1999-03-10 2008-06-15 Asm Scient Inc METHOD FOR DIRECT SEQUENCING OF NUCLEIC ACIDS
US6506594B1 (en) 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
DK1069131T3 (en) * 1999-07-15 2006-06-26 Qiagen Gmbh Process for separating particulate substrates from a solution with minimal particle loss
CA2410950A1 (en) 2000-05-30 2001-12-06 Hans-Michael Wenz Methods for detecting target nucleic acids using coupled ligation and amplification
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
WO2002023163A1 (en) * 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US7723123B1 (en) * 2001-06-05 2010-05-25 Caliper Life Sciences, Inc. Western blot by incorporating an affinity purification zone
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
ES2310237T3 (en) 2002-02-21 2009-01-01 Asm Scientific, Inc. AMPLIFICATION BY RECOMBINASE POLYMERASE.
AU2005333156B2 (en) * 2004-11-03 2011-05-26 Iris Molecular Diagnostics, Inc. Homogeneous analyte detection
WO2008016644A1 (en) * 2006-08-01 2008-02-07 Applera Corporation Detection of analytes and nucleic acids
US7972828B2 (en) * 2006-12-19 2011-07-05 Sigma-Aldrich Co. Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent
CN101382552A (en) * 2007-09-05 2009-03-11 周国华 Method for detecting protein content difference
EP2427771B1 (en) * 2009-05-07 2014-07-09 Biomerieux, Inc Methods for antimicrobial resistance determination
US9562897B2 (en) * 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
US9353406B2 (en) 2010-10-22 2016-05-31 Fluidigm Corporation Universal probe assay methods
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
US20120288857A1 (en) 2011-02-03 2012-11-15 Fluidigm Corporation Multifunctional probe-primers
EP3859011A1 (en) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Methods for forming mixed droplets
CN103635594B (en) 2011-05-09 2018-02-13 富鲁达公司 Probe-based nucleic acid detection
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
JP2016514455A (en) * 2013-03-15 2016-05-23 フリューダイム・コーポレイション Simultaneous detection of target protein and target nucleic acid in a single cell

Also Published As

Publication number Publication date
EP3041957A1 (en) 2016-07-13
CA2922926A1 (en) 2015-03-12
US20150132743A1 (en) 2015-05-14
CN105683397A (en) 2016-06-15
WO2015035087A1 (en) 2015-03-12
US20190292581A1 (en) 2019-09-26
EP3041957A4 (en) 2017-03-29

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